Pay0009298 (gene) Melon (Payzawat) v1

Overview
NamePay0009298
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr04: 15281675 .. 15284706 (+)
RNA-Seq ExpressionPay0009298
SyntenyPay0009298
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATTGAATGTTTAGCAAAATTAGAAGTTCCTATTATAAAATTGCCCAATATTGATAAAAAGGATGATGATATTGATCCTGAAAGCAGCAAATTATGGTTAGAAAGAAGCAAGCAAGTGAGAGATGCTCTTGAAGAATATGGGTGTTTTTTATGTGAGTATGATGATGATGAGAGTAGTCGTTGTTGTAATTCATCTTCATCATCAAAGCTTGATAGTAATTATGAAGAAATTTTCAAGGGATTGAAAGAGCTTTTTGATTTTCCATTAGAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGGTTTTTGGACAGTAGAGCTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGTATTGAAAATGCTTCATCTTATGAAGCTGTTCAGTCTTTTGTCAACCTCTTATGGCCTTCTGGCAATCATCATTTCTGGTAATTATTAATTATGGTATTTGATATACTAAATATGTTTTTTGATTTTTTTTTCTTAGTGTTCTTCATCTCATTATATATATCTAAGTTCAATTGTTTTTATTAACAACTTTTATATATATATATATATATATAGATATATGAAATTTTTACTTTTGGTTTGCTGATCAAAATATTATTTTTTAGCCGAGTGTAAATTATGTTGTTCTTATTTATATATCGTAGAATCTTTTACTCTTTTACTCTTTTTCCACCAAATGTTAGAGTATATTTATATCTTTAATAGTTTTTATACTAAATATATTATACATTGATGATAGGATAATTAGGATCATGTTTAATAAATTATTTAAGTTGCTATTAGTTTTTTTTTTCCTTCAAAATTTATTCTTGATTTCTTACAAATTCTTTACCCATGTGATTTTCACTTGATACTAAAATAAATATTTAAATTCTTAATCAAAATTTAAAAACAAAAATTAGTTTTAAACTCAAAGTTTGTAATTTTTGATAGTGTTGCCAGTAGAAAGTAGACTAGAAAGTGGGGTTTTAGAGTTGAATTTTCAAACCCCAAAAAAATATAAAACAAAACGTTGGTTTTTGATGAGAGAAAACTATTTCGAATAACAAAACAATCGAAAATATTTACAAATATAACAAAATTTTAGAATCTATCAATAACAAATACGAATAAACGTTAATTGATGTCTATCAATATCATCGATATATACAAAAGTCTATCAATATTTATGAGTGATATAATATGAAATTTTGTTATATTTATAAATATTTTAGTTCATTTTGATATAATTTGAGAAGGATTCTCTGAACTTTTTTATTGAAGTTTTGAATGGAAAGATAAAAATAGAAAAATAAAAGGTAAATATACATTTGTAAAGAATTTCTCATATGCCATTTTTGCAGTATTTTGTTTTATTCCTTTGTGGGTTTATTTTTTAAATAAGATAAAATAAAATTGAATTGGACAGTGAAAATTTATTTGCATACTCAAAGAAGATAGCAGAGATGGAGCAATTAGTAAAGAGGATGGTATTTGAAAGCTATGGAGTGGGAAAATACACTGATTCTCATATTGCTTCAACCACTTATCTCCTAAGAACAATGAAATATAGAATTCCAAAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCACACTGACAAAAGTTTCTTCACTATTCTTCATCAATTTGATGGAGTCAATGGCTTGCAAATAAAACCAAAAAATTATGATCAATGGCTTGGAGTTCATTTCTCTCCAAATTACTTCTTGGTCATGGCTGGAGATGCTTGCTTGGTAATACCACCTTCTTTTTTTATTTTTCTTTCTTTTTATTTTACTTTTTGTTTTAATAATATTTTTTCCTTCCGCTTGCATTTTTTTCTTATCGTTGAAAGATCGATATAATATTAAATTTTCCTTTGCCCATAACTTAAACTTTTAAGTCAATCAACGATTTAAAATGATATCAAAGCAAGTGGTCCAAGGGAGGAGGTCATGTATTTAAGCCTCGACAATGTTATTTCCTCTCTAACTAAAATTGATTTTCATTTATTGGGGTATTCTTCTTATATTGATTGTACCACAAGTAAGAATAATGTTGAGTAATATAATATTAAATTTATCTTTTAGATCAAGAATTATTGTCCTAAGAAATCATCTAAATACTTTGCTTCGGATGTTCTTGAGAAATGTGAAGATTCTTCCTTTTGACTTGGTTTTAATGAAACATAAAACATAAGATGAAAAAATAATTACCAAAATAAATCTATCATTTGTTTACAAAAAAAAAAAAAAACAGAAATATGATCACTTTTGTATGTTCTATATAGATACGTATCAATATTTAGGTTTGTTTCAATTGACTTTAATTTCTAAAGAGAGGTTGGGGTCGGACTCCAACTTAACTGAGTGGAATAGGTTATTATAGTCCACTCAAACATTGAATCTCTCATTATAATTATTGAATTATCAATAATCCTAAAATTATTTGAAAATCATCATTGTTCGTAGTATATAATCCTGTTCTCTTTTTAATCAATGATAATCTAGATTATAATAAAATATGTATCTCAAACATTAAATTAAATAATTTATAACTATAACTAGAACCTATACAAATTTTATTGACTAGATTAACCAAATTACCTAACAACTCCAAGAAGTTCTTTAAACATAAAACCATATAAAAAGTCAATAAAGACAAGGTGAAAGTCTATAATCATGAAAAATGTTTCGAAATTCAATTCATGTTTTCTTTATGGTAAGAATTAATAAAAGTTGAATCTCATCAACAATATATATATTTATTATTTTATGGATTAGGCATGGAGCAATGGGAGAATTCACTCAGCCACTCATAGAGTTATAATTGAGGGAAACAAAGAAAGATATTCAACTGCCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGACAAACATCCCTTGAGATTTAAACCTTTTAACCATTATGGATTACTTGGTTATTTTTCCACTGATGATTGTGACAAAATGCCTTCAACTGCCAATGCTTATTGTGGAGTTTGA

mRNA sequence

ATGGGAATTGAATGTTTAGCAAAATTAGAAGTTCCTATTATAAAATTGCCCAATATTGATAAAAAGGATGATGATATTGATCCTGAAAGCAGCAAATTATGGTTAGAAAGAAGCAAGCAAGTGAGAGATGCTCTTGAAGAATATGGGTGTTTTTTATGTGAGTATGATGATGATGAGAGTAGTCGTTGTTGTAATTCATCTTCATCATCAAAGCTTGATAGTAATTATGAAGAAATTTTCAAGGGATTGAAAGAGCTTTTTGATTTTCCATTAGAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGGTTTTTGGACAGTAGAGCTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGTATTGAAAATGCTTCATCTTATGAAGCTGTTCAGTCTTTTGTCAACCTCTTATGGCCTTCTGGCAATCATCATTTCTGTGAAAATTTATTTGCATACTCAAAGAAGATAGCAGAGATGGAGCAATTAGTAAAGAGGATGGTATTTGAAAGCTATGGAGTGGGAAAATACACTGATTCTCATATTGCTTCAACCACTTATCTCCTAAGAACAATGAAATATAGAATTCCAAAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCACACTGACAAAAGTTTCTTCACTATTCTTCATCAATTTGATGGAGTCAATGGCTTGCAAATAAAACCAAAAAATTATGATCAATGGCTTGGAGTTCATTTCTCTCCAAATTACTTCTTGGTCATGGCTGGAGATGCTTGCTTGGCATGGAGCAATGGGAGAATTCACTCAGCCACTCATAGAGTTATAATTGAGGGAAACAAAGAAAGATATTCAACTGCCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGACAAACATCCCTTGAGATTTAAACCTTTTAACCATTATGGATTACTTGGTTATTTTTCCACTGATGATTGTGACAAAATGCCTTCAACTGCCAATGCTTATTGTGGAGTTTGA

Coding sequence (CDS)

ATGGGAATTGAATGTTTAGCAAAATTAGAAGTTCCTATTATAAAATTGCCCAATATTGATAAAAAGGATGATGATATTGATCCTGAAAGCAGCAAATTATGGTTAGAAAGAAGCAAGCAAGTGAGAGATGCTCTTGAAGAATATGGGTGTTTTTTATGTGAGTATGATGATGATGAGAGTAGTCGTTGTTGTAATTCATCTTCATCATCAAAGCTTGATAGTAATTATGAAGAAATTTTCAAGGGATTGAAAGAGCTTTTTGATTTTCCATTAGAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGGTTTTTGGACAGTAGAGCTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGTATTGAAAATGCTTCATCTTATGAAGCTGTTCAGTCTTTTGTCAACCTCTTATGGCCTTCTGGCAATCATCATTTCTGTGAAAATTTATTTGCATACTCAAAGAAGATAGCAGAGATGGAGCAATTAGTAAAGAGGATGGTATTTGAAAGCTATGGAGTGGGAAAATACACTGATTCTCATATTGCTTCAACCACTTATCTCCTAAGAACAATGAAATATAGAATTCCAAAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCACACTGACAAAAGTTTCTTCACTATTCTTCATCAATTTGATGGAGTCAATGGCTTGCAAATAAAACCAAAAAATTATGATCAATGGCTTGGAGTTCATTTCTCTCCAAATTACTTCTTGGTCATGGCTGGAGATGCTTGCTTGGCATGGAGCAATGGGAGAATTCACTCAGCCACTCATAGAGTTATAATTGAGGGAAACAAAGAAAGATATTCAACTGCCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGACAAACATCCCTTGAGATTTAAACCTTTTAACCATTATGGATTACTTGGTTATTTTTCCACTGATGATTGTGACAAAATGCCTTCAACTGCCAATGCTTATTGTGGAGTTTGA

Protein sequence

MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV
Homology
BLAST of Pay0009298 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.4e-61
Identity = 131/317 (41.32%), Postives = 189/317 (59.62%), Query Frame = 0

Query: 15  KLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLDS 74
           +LP ID  D ++ P SSK W E +  V  ALE+YGCF   +D            S +L+ 
Sbjct: 13  QLPVIDFSDQNLKPGSSK-WDEVTADVLKALEDYGCFEASFD----------KLSVELN- 72

Query: 75  NYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQS 134
               +F+ +++LF+ P+ TK +NVS KP+HG+L         L++SLGI +A+  E V  
Sbjct: 73  --RSVFEAMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVND 132

Query: 135 FVNLLWPS-GNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTM 194
           F   LWP  GN    E +  +S+++ E++ +V+RM+ ES+G+  Y D H+ ST YL R M
Sbjct: 133 FTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLM 192

Query: 195 KYRIPKMGEEN------IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNY 254
           KY  P   +++      +G  +HTDK+  TILHQ+  V+GL++K K+ D+W+ V  S + 
Sbjct: 193 KYTSPPDDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDS 252

Query: 255 FLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKG--IIEIPQELVDDKHPL 314
            LVM GD+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P+ELVD +HP 
Sbjct: 253 VLVMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPR 307

Query: 315 RFKPFNHYGLLGYFSTD 323
            FKPF +   L +F T+
Sbjct: 313 IFKPFEYTDFLHFFQTE 307

BLAST of Pay0009298 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.4e-61
Identity = 131/316 (41.46%), Postives = 189/316 (59.81%), Query Frame = 0

Query: 15  KLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLDS 74
           +LP ID  D ++ P SSK W E    V  ALE+YGCF   +D            S +L+ 
Sbjct: 13  QLPVIDFSDQNLKPGSSK-WDEVKADVLKALEDYGCFEAFFD----------KLSVELN- 72

Query: 75  NYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQS 134
               +F+ +++LF+ P+ TK +NVS KP+HG+L         L++SLGI++A+  E V  
Sbjct: 73  --RSVFEAMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVND 132

Query: 135 FVNLLWPS-GNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTM 194
           F   LWP  GN    E +  +S+++ E++ +V+RM+ ES+G+  Y D H+ ST YL R M
Sbjct: 133 FTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLM 192

Query: 195 KYRIPKMGEEN-----IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYF 254
           KY  P   +++     +G  +HTDK+  TILHQ+  V+GL++K K+ D+W+ V  S +  
Sbjct: 193 KYTSPPDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDSV 252

Query: 255 LVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKG--IIEIPQELVDDKHPLR 314
           LVM GD+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P+ELVD +HP  
Sbjct: 253 LVMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRI 306

Query: 315 FKPFNHYGLLGYFSTD 323
           FKPF +   L +F T+
Sbjct: 313 FKPFEYTDFLHFFQTE 306

BLAST of Pay0009298 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.5e-61
Identity = 131/315 (41.59%), Postives = 189/315 (60.00%), Query Frame = 0

Query: 15  KLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLDS 74
           +LP ID  D ++ P SSK W E +  V  ALE+YGCF   +D            S +L+ 
Sbjct: 6   QLPVIDFSDQNLKPGSSK-WDEVTADVLKALEDYGCFEASFD----------KLSVELN- 65

Query: 75  NYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQS 134
               +F+ +++LF+ P+ TK +NVS K +HG+L         L++SLGI +A+  E V  
Sbjct: 66  --RSVFEAMEDLFELPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVND 125

Query: 135 FVNLLWPS-GNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTM 194
           F   LWP  GN    E +  +S+++ E++ +V+RM+ ES+G+ KY D H+ ST YL R M
Sbjct: 126 FTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLM 185

Query: 195 KYRIPKMGEEN----IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFL 254
           KY  P   +++    +G  +HTDK+  TILHQ+  V+GL++K K+ D+W+ V  S +  L
Sbjct: 186 KYTSPPDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDSVL 245

Query: 255 VMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKG--IIEIPQELVDDKHPLRF 314
           VM GD+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P+ELVD +HP  F
Sbjct: 246 VMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIF 298

Query: 315 KPFNHYGLLGYFSTD 323
           KPF +   L +F T+
Sbjct: 306 KPFEYTDFLHFFQTE 298

BLAST of Pay0009298 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 123/313 (39.30%), Postives = 177/313 (56.55%), Query Frame = 0

Query: 14  IKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYD---DDESSRCCNSSSSS 73
           ++LP ID    ++ P ++  W     QVR+ALEEYGCF    D    +     CN     
Sbjct: 7   LRLPVIDLSMKNLKPGTTS-WNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCN----- 66

Query: 74  KLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYE 133
                     KG  EL + PLETKM N +   Y GF       +  L++ +G+   +  E
Sbjct: 67  ----------KG-HELLNLPLETKMLNGNKPEYDGFTS-----IPNLNEGMGVGRITDLE 126

Query: 134 AVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLL 193
            V+ F NL+WP GN  FCE +++Y K++AE++ ++K MVFES+G+ K+ DS   ST YLL
Sbjct: 127 KVERFTNLMWPEGNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLL 186

Query: 194 RTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLV 253
             M+Y+ P     +     H DKS  TI++Q D V GL+ + K+  +W+ +  + N+ +V
Sbjct: 187 HFMRYQQPGKDGRSPALSLHKDKSILTIVNQND-VKGLEFETKD-GEWI-LPTADNH-IV 246

Query: 254 MAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPF 313
           + GD  +AWSNGR+HS  HRV +  N+ R ST+ FS+ K IIE P ELVD++HPL F PF
Sbjct: 247 LLGDCFMAWSNGRLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPF 293

Query: 314 NHYGLLGYFSTDD 324
               LL Y  T +
Sbjct: 307 EITELLAYCFTKE 293

BLAST of Pay0009298 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 5.1e-49
Identity = 134/445 (30.11%), Postives = 197/445 (44.27%), Query Frame = 0

Query: 14  IKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLD 73
           ++LP I+  D  ++P SSK W E    VR ALE++GCF   YD            S +L 
Sbjct: 6   LQLPLINLADKTLEPGSSK-WAEVRSDVRKALEDFGCFEASYD----------KVSLELQ 65

Query: 74  SNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQ 133
              E I K ++ELF  P+ETK +NV  KPY G+L+        L +SLGI NA+  E V 
Sbjct: 66  ---ESIMKTMEELFALPVETKQRNVCPKPYVGYLNHN-----NLSESLGISNANILENVN 125

Query: 134 SFVNLLWP--SGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLR 193
            F   LWP   GN +  + +  +++K+ E++ +V+RMV ES+G+ KY D H+ ST Y +R
Sbjct: 126 EFTQQLWPDGDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMR 185

Query: 194 TMKYRIPKMGEEN----------------------------------------------- 253
            MKY  P  G+ N                                               
Sbjct: 186 LMKYIAPPEGDANTTVDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGAN 245

Query: 254 ------------------------------------------------------------ 313
                                                                       
Sbjct: 246 VNADTGVNDNLNVDAEANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVE 305

Query: 314 ---------IGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDA 338
                    +G   HTDK+ FT+L Q + + GL++K K+ ++W+ V  SPN F+V+AGD+
Sbjct: 306 ANDDNEEKKLGLPCHTDKNLFTVLFQHE-IEGLEVKTKD-EKWIRVKPSPNTFIVIAGDS 365

BLAST of Pay0009298 vs. ExPASy TrEMBL
Match: A0A1S4DWR1 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.5e-144
Identity = 242/243 (99.59%), Postives = 242/243 (99.59%), Query Frame = 0

Query: 95  MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 154
           MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 155 SKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 214
           SKKI EMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 215 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 274
           FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 275 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 334
           GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

Query: 335 CGV 338
           CGV
Sbjct: 241 CGV 243

BLAST of Pay0009298 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 9.0e-134
Identity = 234/337 (69.44%), Postives = 274/337 (81.31%), Query Frame = 0

Query: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60
           MG     K EVP+I         +++ P+S+K W+E    VR ALEEYGCFL EY++D  
Sbjct: 1   MGFRSSTKPEVPVINF-----SIENLGPKSTK-WVETCTHVRAALEEYGCFLAEYENDVE 60

Query: 61  SRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQS 120
                     +LD   + IF GL++LFD PL+TKMKNVSDKPYHGFLD RAPFVLPLH+S
Sbjct: 61  QEV-------ELD---DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHES 120

Query: 121 LGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTD 180
            GIENA+S+E V+SFV L+WPSGNHHFC++LFAY+K ++EME+LVKRMVFESYGV KY D
Sbjct: 121 FGIENAASFEGVESFVKLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYD 180

Query: 181 SHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLG 240
           SHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKSFFTILHQ +GVNGL+IK K  D W+G
Sbjct: 181 SHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWIG 240

Query: 241 VHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVD 300
           + F+ N FLVMAGDACLAWSNGRIHSATHRVIIEG+KERYSTALFSYHKG+IEIP ELVD
Sbjct: 241 LQFNSNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVD 300

Query: 301 DKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
           ++ PL+F PFNHYGLLGYFS ++C+K PSTA AYCGV
Sbjct: 301 EQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV 320

BLAST of Pay0009298 vs. ExPASy TrEMBL
Match: A0A0A0LBU1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G555690 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.5e-133
Identity = 237/258 (91.86%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60
           MGI+CLAKLEVPIIKL N+DKKDDD DPES KLWLERSKQVRDALEEYGCFL EYDDDES
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  SRCCNSSSSSKLDS-NYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQ 120
           S  CNSSSS KL+S NYEEIFKGLKELFD PLETK+KNVSDKPYHGFLD R PFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYT 180
           SLGIENASSYEAVQ FVNLLWPSGNHHFCE LFAYSKKI+EMEQLVKRMVFESYGVGKY 
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240
           DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL
Sbjct: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240

Query: 241 GVHFSPNYFLVMAGDACL 258
           GV FSPN FLVMAGDACL
Sbjct: 241 GVQFSPNSFLVMAGDACL 258

BLAST of Pay0009298 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.4e-133
Identity = 239/323 (73.99%), Postives = 268/323 (82.97%), Query Frame = 0

Query: 15  KLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLDS 74
           KLP I+   +++ PES+  WLE    +R ALEEYGCFL E+           S++ +LD 
Sbjct: 11  KLPVINLSIENVSPESTN-WLEICSHLRAALEEYGCFLGEF----------GSATPELD- 70

Query: 75  NYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQS 134
             +EIFKGL+ELFD PLETKMKNVSDKPYHGFLD R PFVLPLHQS GIEN+ S+EAVQS
Sbjct: 71  --DEIFKGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQS 130

Query: 135 FVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMK 194
           FVNL+WPSGN  FCEN+F+Y+K ++EME+LVK MVFESYGV KY DSHIASTTYLLRTMK
Sbjct: 131 FVNLMWPSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMK 190

Query: 195 YRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGD 254
           YRIP+MGEENIGA+AHTDKSFFTILHQ + VNGL+IK K+   WLGV F+PN FLVMAGD
Sbjct: 191 YRIPEMGEENIGADAHTDKSFFTILHQ-NEVNGLEIKTKD-GHWLGVEFTPNSFLVMAGD 250

Query: 255 ACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYG 314
           ACLAWSNGRIHSATHRV+IEG KERYSTALFSYHKGIIEIP ELVD+KHPLRF PFNHYG
Sbjct: 251 ACLAWSNGRIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYG 310

Query: 315 LLGYFSTDDCDKMPSTANAYCGV 338
           LL YFST D  KMPSTA AYCGV
Sbjct: 311 LLAYFSTHDSHKMPSTAKAYCGV 317

BLAST of Pay0009298 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 7.6e-133
Identity = 234/337 (69.44%), Postives = 273/337 (81.01%), Query Frame = 0

Query: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60
           MG     K EVP I         +++ P+S+K W+E    VR ALEEYGCFL E +    
Sbjct: 1   MGFRSSKKPEVPAINF-----SIENLRPKSTK-WVETCSHVRAALEEYGCFLAECE---- 60

Query: 61  SRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQS 120
                  ++ +LD   + IF GL++LFD PLETKMKNVSDKPYHGFLD RAPFVLPLH+S
Sbjct: 61  -------NNVELD---DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHES 120

Query: 121 LGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTD 180
            GIENA+S+E V+SFV L+WPSGNHHFCE+LFAY+K ++EME+LVKRMVFESYGV KY D
Sbjct: 121 FGIENAASFEGVESFVKLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYD 180

Query: 181 SHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLG 240
           SHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKSFFTILHQ +GVNGL+IK K  D W+G
Sbjct: 181 SHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWIG 240

Query: 241 VHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVD 300
           + F+PN FLVMAGDACLAWSNGRIHSATHRV+IEG+KERYSTALFSYHKG+IEIP ELVD
Sbjct: 241 LQFNPNSFLVMAGDACLAWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVD 300

Query: 301 DKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
           ++ PL+F PFNHYGLLGYFS ++C+K PSTA AYCGV
Sbjct: 301 EQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV 316

BLAST of Pay0009298 vs. NCBI nr
Match: XP_011651543.2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8650761.1 hypothetical protein Csa_017427 [Cucumis sativus])

HSP 1 Score: 653.7 bits (1685), Expect = 8.8e-184
Identity = 313/338 (92.60%), Postives = 320/338 (94.67%), Query Frame = 0

Query: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60
           MGI+CLAKLEVPIIKL N+DKKDDD DPES KLWLERSKQVRDALEEYGCFL EYDDDES
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  SRCCNSSSSSKLDS-NYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQ 120
           S  CNSSSS KL+S NYEEIFKGLKELFD PLETK+KNVSDKPYHGFLD R PFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYT 180
           SLGIENASSYEAVQ FVNLLWPSGNHHFCE LFAYSKKI+EMEQLVKRMVFESYGVGKY 
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240
           DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL
Sbjct: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240

Query: 241 GVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELV 300
           GV FSPN FLVMAGDACLAWSNGRIHSATHRVIIEGNKERYST LFSYHKGIIEIPQELV
Sbjct: 241 GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV 300

Query: 301 DDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
           D+KHPLRF PFNHYGLLGYFSTDDCDK+PSTANAYCGV
Sbjct: 301 DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338

BLAST of Pay0009298 vs. NCBI nr
Match: XP_038878200.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida])

HSP 1 Score: 552.4 bits (1422), Expect = 2.8e-153
Identity = 267/337 (79.23%), Postives = 295/337 (87.54%), Query Frame = 0

Query: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60
           MGI+  AK EVP+IK     + D+D+ P+S+K WLE   QVR ALEEYGCFL EY+    
Sbjct: 1   MGIQSSAKSEVPVIKFS--IENDEDLHPQSTK-WLETCSQVRAALEEYGCFLAEYE---- 60

Query: 61  SRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQS 120
                 S+SSKL   Y+EIFKGLKELFD PLETKMKNVSDKPYHGFLD  APFVLPLHQS
Sbjct: 61  ------SASSKL---YDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQS 120

Query: 121 LGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTD 180
           LGIENA+S++AV+SF+ LLWPSGNHHFCENLF+YS+ I+EMEQLVK+MVFESYGVGKY D
Sbjct: 121 LGIENATSFQAVESFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYD 180

Query: 181 SHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLG 240
           SHIASTTYLLRTMKYRIP+MGEENIGAEAHTDKSFFTILHQ +GVNGL+IKPKN D WL 
Sbjct: 181 SHIASTTYLLRTMKYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLR 240

Query: 241 VHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVD 300
           + FSPN FLVMAGDACLAWSNGRIHSATHRVIIEG+KERYSTALFSYHKGIIEIPQELVD
Sbjct: 241 LQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVD 300

Query: 301 DKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
           ++HPLRF PFNHYGLLGYFSTD+C+KMPSTANAYCGV
Sbjct: 301 EQHPLRFNPFNHYGLLGYFSTDECEKMPSTANAYCGV 321

BLAST of Pay0009298 vs. NCBI nr
Match: XP_016900413.1 (PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo])

HSP 1 Score: 521.5 bits (1342), Expect = 5.2e-144
Identity = 242/243 (99.59%), Postives = 242/243 (99.59%), Query Frame = 0

Query: 95  MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 154
           MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 155 SKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 214
           SKKI EMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 215 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 274
           FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 275 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 334
           GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

Query: 335 CGV 338
           CGV
Sbjct: 241 CGV 243

BLAST of Pay0009298 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491.5 bits (1264), Expect = 5.8e-135
Identity = 237/337 (70.33%), Postives = 274/337 (81.31%), Query Frame = 0

Query: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60
           MG       EVP+I         +++ P+S+K W+E    VR ALEEYGCFL EY++D  
Sbjct: 1   MGFRSSTNPEVPVISF-----SIENLRPKSTK-WVETCSHVRAALEEYGCFLAEYENDVE 60

Query: 61  SRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQS 120
                     +LD   + IF GL++LFD PLETKMKNVSDKPYHGFLD RAPFVLPLH+S
Sbjct: 61  QEV-------ELD---DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHES 120

Query: 121 LGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTD 180
            GIENA+S+E V+SFV L+WPSGNHHFCE+LFAY+K ++EME+LVKRMVFESYGV KY D
Sbjct: 121 FGIENAASFEGVESFVKLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYD 180

Query: 181 SHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLG 240
           SHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKSFFTILHQ +GVNGL+IK K  D W+G
Sbjct: 181 SHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWIG 240

Query: 241 VHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVD 300
           + F+PN FLVMAGDACLAWSNGRIHSATHRVIIEG+KERYSTALFSYHKG+IEIP ELVD
Sbjct: 241 LQFNPNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELVD 300

Query: 301 DKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
           ++ PL+F PFNHYGLLGYFS +DC+K PSTA AYCGV
Sbjct: 301 EQFPLKFNPFNHYGLLGYFSKEDCEKTPSTAGAYCGV 320

BLAST of Pay0009298 vs. NCBI nr
Match: KAG6606775.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 489.6 bits (1259), Expect = 2.2e-134
Identity = 235/337 (69.73%), Postives = 276/337 (81.90%), Query Frame = 0

Query: 1   MGIECLAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDES 60
           MG     K EVP+I         +++ P+S+K W+E    VR ALEEYGCFL EY++D  
Sbjct: 1   MGFRSSTKPEVPVINF-----SIENLRPKSTK-WVETCTHVRAALEEYGCFLAEYENDVE 60

Query: 61  SRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQS 120
                     +LD   + IF GL++LFD PL+TKMKNVSDKPYHGFLD RAPFVLPLH+S
Sbjct: 61  QEV-------ELD---DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHES 120

Query: 121 LGIENASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTD 180
            GIENA+S+E V+SFV L+WPSGNHHFC++LFAY+K ++EME+LVKRMVFESYGVGKY D
Sbjct: 121 FGIENAASFEGVESFVKLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYD 180

Query: 181 SHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLG 240
           SHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKSFFTILHQ +GVNGL+IK K  D+W+ 
Sbjct: 181 SHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDRWIR 240

Query: 241 VHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVD 300
           + F+PN FLVMAGDACLAWSNGRIHSATHRVIIEG+KERYSTALFSYHKG+IEIP ELVD
Sbjct: 241 LQFNPNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVD 300

Query: 301 DKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
           ++ PL+F PFNHYGLLGYFS ++C+K PSTA AYCGV
Sbjct: 301 EQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV 320

BLAST of Pay0009298 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 2.8e-79
Identity = 145/322 (45.03%), Postives = 207/322 (64.29%), Query Frame = 0

Query: 15  KLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLDS 74
           K+P +D   +D+ P  +K W    + +R ALEEYGCF+ +  D            + LD 
Sbjct: 4   KIPTLDFSREDLKP-GTKYWESTRENIRQALEEYGCFIIDLKD-----------KTPLDL 63

Query: 75  NYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQS 134
             + +F  L +LFD P +TKMKN  DKP +G++  + P  LPLH+SLGI+NA+S EA +S
Sbjct: 64  -LDRVFGSLVDLFDLPTQTKMKNKYDKPLNGYV-GQIP-ALPLHESLGIDNATSLEATRS 123

Query: 135 FVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMK 194
           F  L+WP GN HF E L+ Y++  AE++Q+V RMVF+SY V KY D +I STTYLLR +K
Sbjct: 124 FTGLMWPQGNEHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLK 183

Query: 195 YRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGD 254
            R P      +G   HTDKSF TILHQ D VNGL+++ +  ++      SP+ F+V+AGD
Sbjct: 184 NRAPNNENPTLGFVTHTDKSFTTILHQ-DQVNGLEMETREGERININLSSPSLFMVVAGD 243

Query: 255 ACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYG 314
           A +AWSN R+ S  H+V++ G  +RYS  +F+++ G +++P+EL+D +HPL +KPF+H G
Sbjct: 244 ALMAWSNDRVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLG 303

Query: 315 LLGYFSTDDCDKMPSTANAYCG 337
           LL ++ TD   K      AYCG
Sbjct: 304 LLRFYRTDTGYKSECPIKAYCG 309

BLAST of Pay0009298 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 5.3e-78
Identity = 141/332 (42.47%), Postives = 207/332 (62.35%), Query Frame = 0

Query: 6   LAKLEVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCN 65
           L   +VPI+ L +      ++ P +S  W   S++  +ALEEYGCFL  YD         
Sbjct: 5   LKTTKVPILDLTS----QQELKPNTS-TWRSVSREACEALEEYGCFLAVYD--------- 64

Query: 66  SSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIEN 125
              + +LD   + IF   +ELFD P ETK KNV++KPYHG++      V+PLH+ LG++ 
Sbjct: 65  -GVTQQLD---DSIFAAAEELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDY 124

Query: 126 ASSYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIAS 185
            ++ E  Q F +L+WP GN  FC  +  +S  +AE+++LV RM+FE+YGV K+ +SH+ S
Sbjct: 125 VTNKEIAQRFTHLMWPQGNDRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGS 184

Query: 186 TTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSP 245
            TYLL+ +KY  P           HTDK+F +ILHQ D VNGL++K K+  +W+ +   P
Sbjct: 185 KTYLLKFLKYLAPPESISMPAFPQHTDKTFLSILHQND-VNGLEVKSKD-GEWISLQLPP 244

Query: 246 NYFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVDDKHPL 305
             ++VMAGD  + WSN RI S  HRV +EG+K RY+  LFS+   ++ IP+ELVDDKHPL
Sbjct: 245 KSYVVMAGDISMGWSNDRIRSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPL 304

Query: 306 RFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
            +KPF++  L+ +++T +  +  ST  AYCG+
Sbjct: 305 MYKPFDNIALINFYTTKEGREANSTLKAYCGI 314

BLAST of Pay0009298 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 8.8e-65
Identity = 131/333 (39.34%), Postives = 192/333 (57.66%), Query Frame = 0

Query: 10  EVPIIKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSS 69
           E+PII L + + K      ++++LW      VR+A+E +G F+ EY++  +         
Sbjct: 8   ELPIINLSDKNLK------QNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGL------- 67

Query: 70  SKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSY 129
                 ++ I +  KEL D P+E K+KN + K  HG++   +    P+H+ LGI+  +  
Sbjct: 68  ------HQSILEAAKELLDLPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDV 127

Query: 130 EAVQSFVNLLWP---SGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIAST 189
           +  + F  L+WP     N  FCE + AY+K  AE+EQLV RM+FESY V KYT+ +I  T
Sbjct: 128 QQCRGFSRLMWPDDHDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGT 187

Query: 190 TYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPN 249
            YLLR +KYR    GE N    +HTDKSF +ILHQ + + GL +K +  D W     SP 
Sbjct: 188 RYLLRLLKYRRLPNGEPNRKFISHTDKSFISILHQ-NHITGLMLKSEKEDVWYHFTPSPT 247

Query: 250 YFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVDDKHPLR 309
            F+V+AGDA +AWSN RI +  H+V +E  + RYS   FS+ +G+I  P+E+VD  HPL 
Sbjct: 248 RFVVIAGDAIMAWSNDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLA 307

Query: 310 FKPFNHYGLLGYFSTDDCDKMP--STANAYCGV 338
           + PF+H GLL Y+ T +       +   AYCG+
Sbjct: 308 YNPFHHDGLLEYYETLEVHLKAHRTMTKAYCGI 319

BLAST of Pay0009298 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-64
Identity = 130/332 (39.16%), Postives = 198/332 (59.64%), Query Frame = 0

Query: 10  EVPI--IKLPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSS 69
           E P+  ++LP ID  + ++ P   +  L R+  V+ AL++YG F   +D           
Sbjct: 4   ETPLLPLRLPVIDFSNKNLKPGEPEWDLTRA-DVQKALQDYGYFEASFDRIPFEL----- 63

Query: 70  SSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENAS 129
                    + +F  L+ELFD PL+TK++NVS KP+HG++  + P V PL++S+GI+++ 
Sbjct: 64  --------RKSVFGALEELFDLPLQTKLRNVSKKPFHGYV-GQYPMV-PLYESMGIDDSD 123

Query: 130 SYEAVQSFVNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVGKYTDSHIASTT 189
             E V +F   LWP GN  F   + ++SKK++E++  ++RM+ ES+G+ KY D H+ ST 
Sbjct: 124 IAEKVDAFTEKLWPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTN 183

Query: 190 YLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNY 249
           YLLR MKY+ P   E  +G  AHTDK+  TIL+Q + V GL+++ K+   W+ V  + + 
Sbjct: 184 YLLRVMKYKGPDTEETKVGLNAHTDKNIVTILYQ-NHVEGLEVQTKD-KNWIKVKPTQDS 243

Query: 250 FLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKG--IIEIPQELVDDKHPL 309
           F VM GD+  A  NGR+HS  HRV++ G + RYS  LFS  K   I+  P ELVD++HP 
Sbjct: 244 FTVMIGDSLYALLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPR 303

Query: 310 RFKPFNHYGLLGYFSTDDCDKMPSTANAYCGV 338
            FKPF+H   L ++ T+   +  S    YCG+
Sbjct: 304 LFKPFDHVEFLQFYYTEAGQRSQSALKTYCGI 317

BLAST of Pay0009298 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 236.9 bits (603), Expect = 2.4e-62
Identity = 132/327 (40.37%), Postives = 190/327 (58.10%), Query Frame = 0

Query: 16  LPNIDKKDDDIDPESSKLWLERSKQVRDALEEYGCFLCEYDDDESSRCCNSSSSSKLDSN 75
           LP +D   D +   +S  W+     VR A+E  G F+ E+          S  SS L  N
Sbjct: 9   LPILDFSSDKLVRGTSH-WITTRDDVRRAMEGQGWFVAEF----------SGVSSDLRDN 68

Query: 76  YEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSF 135
              +  G+KE++  P + K+KN + K  HG++ S       +H+SLGI+ A+  +A + F
Sbjct: 69  ---LLAGMKEMYYLPDQIKIKNENHKASHGYM-SMVVDDYRIHESLGIDYATELQACKDF 128

Query: 136 VNLLWPSGNHHFCENLFAYSKKIAEMEQLVKRMVFESYGVG--KYTDSHIASTTYLLRTM 195
             LLWP GN  FC+    Y+  +AE++Q V RM++ESYG+   K++ SH  ST YLLR +
Sbjct: 129 SKLLWPQGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRML 188

Query: 196 KYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAG 255
            YR  + GE N G  +HTDKSF +ILHQ + V GLQ+K     QW+G + SP  F+V++G
Sbjct: 189 SYRRQQNGEANTGFVSHTDKSFMSILHQ-NHVGGLQLKTMT-GQWVGFNPSPTRFVVLSG 248

Query: 256 DACLAWSNGRIHSATHRVIIEGNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHY 315
               AWSN RI +  H+V++  ++ RYS   FS+HKG I  P+ELVDD+HPLR+ PF H 
Sbjct: 249 MGLTAWSNDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHD 308

Query: 316 GLLGYFSTDDCDKMPSTAN---AYCGV 338
           GLL ++ +       S+ +    YCGV
Sbjct: 309 GLLRFYESYINSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZTA33.4e-6141.32Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q945B64.4e-6141.46Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q944Z57.5e-6141.59Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ361.8e-5439.30Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q945B55.1e-4930.112-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
A0A1S4DWR12.5e-14499.592-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1GA179.0e-13469.44probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A0A0LBU11.5e-13391.86Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6J1DH703.4e-13373.992-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1K6A37.6e-13369.442-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_011651543.28.8e-18492.60probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8650761... [more]
XP_038878200.12.8e-15379.232-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida][more]
XP_016900413.15.2e-14499.59PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo][more]
XP_023525673.15.8e-13570.332-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo][more]
KAG6606775.12.2e-13469.73putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
AT1G52790.12.8e-7945.032-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.15.3e-7842.472-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G15540.18.8e-6539.342-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.11.5e-6439.162-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.12.4e-6240.372-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 203..285
e-value: 8.9E-16
score: 58.2
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 4..319
e-value: 5.0E-48
score: 166.0
NoneNo IPR availablePANTHERPTHR47990:SF172-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKEcoord: 12..336
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 12..336
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 10..318
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 185..288
score: 9.088635

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0009298.1Pay0009298.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding