Pay0009178 (gene) Melon (Payzawat) v1

Overview
NamePay0009178
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptiontRNA-intron lyase
Locationchr11: 26668464 .. 26671515 (-)
RNA-Seq ExpressionPay0009178
SyntenyPay0009178
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAAAATTTAGATCACTGCATCATTTTAGATTATTGCCACACATTTGTATCCTTAATTTTTGTTAGAAATTGTAAGTATGTAGTTAGAGGTTGGGAGGTTCAACCACCACTCTCCAATGTTACAAATAATATCTTTATTCTTTTAATTTTATCTAATCTATGATCTCCAACTACTTTCCTTTTTCTTTTAACTTACTCTCCCCGCCGCCGCACTCTTCTTTTTCTCTTTTATCTCGCGTTGTTGCCGTCGATTTCACTGTTTCACCTCCGGCTTCAATGGCGCCGAGATGGAAAGGGAAGGCTTCAGAAGCCAAAGCTCTAGCTGATCCCATCTCCAGTACTGTACTTCACCTTCAATCTTCTTTCATACAGTCCAACGCTCGAGGATTATTATCTGGTTCTACCATACTTTATCTAGCCGACGAAGAACAAACCGATCTTCTTAATCGCACTTGTTTCGGCCGACAAATTCTTACTGCTGAGAAGGACAAACAATGGTTTCAATTTGGCATGGAGGAGGCTTTTTACTTGTCCTATCACTTGAATTGCCTCAAAATTGTCGATGGACAAGACTGTGAAATGAATTTTCAAGAGCTTTGGCGGTATATGAAGACCCAAAAGGCTACATTCCCTGAGTTTTACATTGCTTACCGACATCTTCGATTGAAGAATTGGGTTGTTAGACCTGGATCTCAATATGGGGTTGATTTTGTGGCGTATCGTCATCATCCGGCTCTTGTTCACTCTGAATTTGCTGTTCTGGTGTTGTCTGAAGGAAGAGACAGTAATGGAAATGGGCGATTGAGGGTTTGGTCTGATTTTCTTTGCACAATTCGGCTTTGTGGAAGTGTTGCTAAAACGTTGCTAGTTCTTTCCGTCAATGATAATGGAAGTGAATTTGTTTCTCCTTCGTGTTTGGACAGTTACTCCATTGAAGAACGCACAGTTACGAGATGGAGTCCAGAACAGTGCCGTGAAAATCCTGCAGAAGTGTGAAATAGACCAAGTAAGATATAATTTAGGGTCTTTTATCTGTTCTTCTTTTTTTTTTTTTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGATAATTTCTGATTGTTCTTCATGTACATCGAGAAACAACTATCTAGGGCTATACAACAGATCTAGTTTTTGATGGGTTTTGAAGATGATAGAGTAAGGGATAGTTCCAATTACTTCGAACTTGAATGCTTGGGGGTAAGGGATAGCTCAAAAAGATATTTCTTTCTGGAATAACTACAATTACACAACTAAACAACGTTGAATGTAAAAGATCCTCAAAGTATATACACTGACAATCACATGCTCCATATCAAAGTATGCCAAATCCTAAGAGCTAATATTAGAAAGAGATTCTAGAAGTGACTACCAAAGTATGTTAAATTATTTCTAAACTCAAAAGTTTGCTCTTGGGAGACTGGAGAGTATGATGAATCTCTTATCTGTATTCTTAACATTTGAGTAACTATGTAGCTGGGTTGTGAATTCGCCATCTGCACTTTTCTCTTGGAGAATTCATCATTATTGATTGCTAGGTTTTCCTTGATGCTCCAATTGCAAACAACAAAATGATGAAGACGATGTTATTTGTTAGAGACATCTTCCTCCATTGTTGCCATAACAGAGTCCCTCCTGAAAATTGTATCATTTGGCAGATCGGCTACTGCCAGTTTAAAATGTCTCATTATATAGACATAACATCTCTCACCTCTTGATGGAATCTACTGTCAAAGACAAGGATACATTTAGCAGTAATTTTAAAAAGTAAAAATCTGCTTGATCCCCAATTCTGAAAATAGTTGGGGGGGGGGGGGGGTGAGGGGCTTTGTTAGGGGTTTAGTAGGGTTTTGGGGATTGGTGTGGAGGTAGGGAGTTTTTGGTAGTGAGAGAATTCACCAAAGGTGAGATTTGGATCTCTCGAACTGTCGGGGTTGTGTTTTGTTCTTTGCAATTTGTGGTCTTGTTCTTAGACTTGATTATCGTTGTAACCTTACTGAACTTTGCTGGTATCTTTGATTATATATGTTGTACAAAGGGGATTTGCGTCTGTTTCTCAGATTGTTAGGTCTTTCTGTGTGCATGAATCTTTAAGTTTTTGATATTTAAAATAACTCTGAGACGTGTCTTAAATCAAAATCATTTCGTACAATAAAAGTACAAAATTGATTTCAACAATTTTAAAAATCACTACTTAACATGTCTTAAACAAACCAGATCGAAAATATTAGATAACAGAGCCAAATTCTTTACATCAATCTGGCTATTACTCTAACGTACTTTCAAAAGAATGTGACTTGCAACTGTTACTTTCTCTTTTGCTTAGGAGCATACATTAATTGACTGACGGGAAAGGTATTGTGTAGAAAGGTGGATGTACTTGAAAGAATTGTAGCTTTGCTAGTTGATAATCTTTTGAAATAGGCCTCTCATTATTGCTAATGCTTTTTATGCAGCTTTTAAGGTATTCTCTTATGAAGATGAACTATGTGCGGAAAATGGAAACCAAGAATTTTGAAGATAATGTAAATGATGCAAGAAAAGGAGGCAGAGGTTTTAACATGTCAAACACTGCAACACGAGGAACTACTGAGAAATCATATCCGTTTCAATAGAGGTTACTACCATTATGTTATATTGCCATGGAAGGAAAACCGTGAGAATATGACTAGGAGGATGGTCTCCTCCATTGTTGTGTTGCTACAATCAAGACATTAGTGGTTTCAATCCTCCTAACTCAAAAGGAAGAAGATTGTTGTGTTATTCATGCTAACCAACAATATTTCTTGGCCACTCACGCTTCAATTTATCGGCTTTATTTATTAGGTGAGATGTCATTTTATCTTAAACATGAAAAGAATCTTATGAGTATGTGCATTAAAGATTTTGATAGAAGAGTAGAGAAATATGATTATGGTAGATTATTCACAACAATCAAAGAGCTTTCACATTATTTAACGGAATAATTATTGTAGGGTGATATATATAGTTGAAATCTTTCGAATTCCTTTCAGGATCACTA

mRNA sequence

ACAAAATTTAGATCACTGCATCATTTTAGATTATTGCCACACATTTGTATCCTTAATTTTTGTTAGAAATTGTAAGTATGTAGTTAGAGGTTGGGAGGTTCAACCACCACTCTCCAATGTTACAAATAATATCTTTATTCTTTTAATTTTATCTAATCTATGATCTCCAACTACTTTCCTTTTTCTTTTAACTTACTCTCCCCGCCGCCGCACTCTTCTTTTTCTCTTTTATCTCGCGTTGTTGCCGTCGATTTCACTGTTTCACCTCCGGCTTCAATGGCGCCGAGATGGAAAGGGAAGGCTTCAGAAGCCAAAGCTCTAGCTGATCCCATCTCCAGTACTGTACTTCACCTTCAATCTTCTTTCATACAGTCCAACGCTCGAGGATTATTATCTGGTTCTACCATACTTTATCTAGCCGACGAAGAACAAACCGATCTTCTTAATCGCACTTGTTTCGGCCGACAAATTCTTACTGCTGAGAAGGACAAACAATGGTTTCAATTTGGCATGGAGGAGGCTTTTTACTTGTCCTATCACTTGAATTGCCTCAAAATTGTCGATGGACAAGACTGTGAAATGAATTTTCAAGAGCTTTGGCGGTATATGAAGACCCAAAAGGCTACATTCCCTGAGTTTTACATTGCTTACCGACATCTTCGATTGAAGAATTGGGTTGTTAGACCTGGATCTCAATATGGGGTTGATTTTGTGGCGTATCGTCATCATCCGGCTCTTGTTCACTCTGAATTTGCTGTTCTGGTGTTGTCTGAAGGAAGAGACAGTAATGGAAATGGGCGATTGAGGGTTTGGTCTGATTTTCTTTGCACAATTCGGCTTTGTGGAAGTGTTGCTAAAACGTTGCTAGTTCTTTCCGTCAATGATAATGGAAGTGAATTTGTTTCTCCTTCGTGTTTGGACAGTTACTCCATTGAAGAACGCACAGTTACGAGATGGAGTCCAGAACAGTGCCGTGAAAATCCTGCAGAAGTGTGAAATAGACCAACTTTTAAGGTATTCTCTTATGAAGATGAACTATGTGCGGAAAATGGAAACCAAGAATTTTGAAGATAATGTAAATGATGCAAGAAAAGGAGGCAGAGGTTTTAACATGTCAAACACTGCAACACGAGGAACTACTGAGAAATCATATCCGTTTCAATAGAGGTTACTACCATTATGTTATATTGCCATGGAAGGAAAACCGTGAGAATATGACTAGGAGGATGGTCTCCTCCATTGTTGTGTTGCTACAATCAAGACATTAGTGGTTTCAATCCTCCTAACTCAAAAGGAAGAAGATTGTTGTGTTATTCATGCTAACCAACAATATTTCTTGGCCACTCACGCTTCAATTTATCGGCTTTATTTATTAGGTGAGATGTCATTTTATCTTAAACATGAAAAGAATCTTATGAGTATGTGCATTAAAGATTTTGATAGAAGAGTAGAGAAATATGATTATGGTAGATTATTCACAACAATCAAAGAGCTTTCACATTATTTAACGGAATAATTATTGTAGGGTGATATATATAGTTGAAATCTTTCGAATTCCTTTCAGGATCACTA

Coding sequence (CDS)

ATGATCTCCAACTACTTTCCTTTTTCTTTTAACTTACTCTCCCCGCCGCCGCACTCTTCTTTTTCTCTTTTATCTCGCGTTGTTGCCGTCGATTTCACTGTTTCACCTCCGGCTTCAATGGCGCCGAGATGGAAAGGGAAGGCTTCAGAAGCCAAAGCTCTAGCTGATCCCATCTCCAGTACTGTACTTCACCTTCAATCTTCTTTCATACAGTCCAACGCTCGAGGATTATTATCTGGTTCTACCATACTTTATCTAGCCGACGAAGAACAAACCGATCTTCTTAATCGCACTTGTTTCGGCCGACAAATTCTTACTGCTGAGAAGGACAAACAATGGTTTCAATTTGGCATGGAGGAGGCTTTTTACTTGTCCTATCACTTGAATTGCCTCAAAATTGTCGATGGACAAGACTGTGAAATGAATTTTCAAGAGCTTTGGCGGTATATGAAGACCCAAAAGGCTACATTCCCTGAGTTTTACATTGCTTACCGACATCTTCGATTGAAGAATTGGGTTGTTAGACCTGGATCTCAATATGGGGTTGATTTTGTGGCGTATCGTCATCATCCGGCTCTTGTTCACTCTGAATTTGCTGTTCTGGTGTTGTCTGAAGGAAGAGACAGTAATGGAAATGGGCGATTGAGGGTTTGGTCTGATTTTCTTTGCACAATTCGGCTTTGTGGAAGTGTTGCTAAAACGTTGCTAGTTCTTTCCGTCAATGATAATGGAAGTGAATTTGTTTCTCCTTCGTGTTTGGACAGTTACTCCATTGAAGAACGCACAGTTACGAGATGGAGTCCAGAACAGTGCCGTGAAAATCCTGCAGAAGTGTGA

Protein sequence

MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV
Homology
BLAST of Pay0009178 vs. ExPASy Swiss-Prot
Match: Q9M1E8 (tRNA-splicing endonuclease subunit Sen2-1 OS=Arabidopsis thaliana OX=3702 GN=SEN1 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.5e-67
Identity = 132/238 (55.46%), Postives = 165/238 (69.33%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWK K +EAKALA+P+S TV  L+SS  Q+ A G LS   +L   + E+ +LL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKI-VDGQDCEMNFQELWRYMKTQKATFP 159
           FGR ++ AEKDK+W Q   EEAF+L Y L C+KI + G+  E N  +LWR M + K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 160 EFYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVW 219
             Y AY HLR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S G    GN RL+VW
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIG----GNDRLKVW 180

Query: 220 SDFLCTIRLCGSVAKTLLVLSVND--NGSEFVSPSCLDSYSIEERTVTRWSPEQCREN 275
           SD  C++RL GSVAK+LLVL VN   N  +   P CL+ Y++EE+T+ RWSPE  RE+
Sbjct: 181 SDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSRED 233

BLAST of Pay0009178 vs. ExPASy Swiss-Prot
Match: Q9LSS3 (tRNA-splicing endonuclease subunit Sen2-2 OS=Arabidopsis thaliana OX=3702 GN=SEN2 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.6e-67
Identity = 128/237 (54.01%), Postives = 164/237 (69.20%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWK K +EAKALA+PIS +V  LQ S  ++ + G LS   +L   + EQ +LL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 159
           FGR +L+AEK K+W Q   EEAF+L Y+L C+KI     C  N  + W YMK+++  FP 
Sbjct: 61  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 120

Query: 160 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 219
           F+ AY HLR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV S   D     RLRVWS
Sbjct: 121 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLVQSGDSD-----RLRVWS 180

Query: 220 DFLCTIRLCGSVAKTLLVLSVNDN--GSEFVSPSCLDSYSIEERTVTRWSPEQCREN 275
           D  C +RL GSVAKTLL L VN N  G +     CL+++++EE+T++RWSPE  RE+
Sbjct: 181 DIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 232

BLAST of Pay0009178 vs. ExPASy Swiss-Prot
Match: Q5Z6B1 (Probable tRNA-splicing endonuclease subunit Sen2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0530700 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.2e-61
Identity = 125/245 (51.02%), Postives = 157/245 (64.08%), Query Frame = 0

Query: 42  PRWK----GKASEAKALADPISSTVLHLQSSFIQSNARGLLS---GSTILYLADEEQTDL 101
           PRWK    GK   + A A+P+S+ V  L++SFI S    +LS   GS +L +   EQ  +
Sbjct: 6   PRWKKGKDGKDFASLAAANPMSAIVSELKASFISSKPVAILSGPGGSAVLGVG-PEQAVI 65

Query: 102 LNRTCFGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQK 161
           LNR  FG  I  A   K WFQ   EE FYL + LNC+++    + +M+  ELW Y ++  
Sbjct: 66  LNRAAFGHAIENATAQKHWFQLSPEEVFYLCHALNCIRVDSLDNKQMSEIELWDYFRSGS 125

Query: 162 ATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRD-SNGNG 221
            +FPE Y AY HLRLKNWVVR G QYG DFVAYRHHPALVHSEFAV+V+ EG +  N  G
Sbjct: 126 ESFPEMYKAYAHLRLKNWVVRSGLQYGADFVAYRHHPALVHSEFAVVVVPEGAEFGNRCG 185

Query: 222 RLRVWSDFLCTIRLCGSVAKTLLVLSVNDNGS-EFVSPSCLDSYSIEERTVTRWSPEQCR 278
           RL VWSD LC +R  GSVAKTLLVL+++ +   E  SP CL+   + ERT+TRW  +QCR
Sbjct: 186 RLEVWSDLLCALRASGSVAKTLLVLTISSSSKCELSSPDCLEQLVVHERTITRWILQQCR 245

BLAST of Pay0009178 vs. ExPASy Swiss-Prot
Match: Q6P7W5 (tRNA-splicing endonuclease subunit Sen2 OS=Mus musculus OX=10090 GN=Tsen2 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-17
Identity = 51/174 (29.31%), Postives = 86/174 (49.43%), Query Frame = 0

Query: 112 QWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKN 171
           ++ Q  +EEAF+L+Y L CL I   ++  +   +LW+     + TF   Y+AY + R K 
Sbjct: 290 EYLQLSLEEAFFLAYALGCLSIYYEKE-PLTIVKLWQAFTAVQPTFRTTYMAYHYFRSKG 349

Query: 172 WVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLR---VWSDFLCTIRLC 231
           WV + G +YG D + YR  P   H+ ++V++  E  D N  G LR    W       R+ 
Sbjct: 350 WVPKVGLKYGTDLLLYRKGPPFYHASYSVII--ELLDDNYEGSLRRPFSWKSLAALSRVS 409

Query: 232 GSVAKTL----LVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           G+V+K L    L+        +  +P C+    ++E  ++RW   + R +  E+
Sbjct: 410 GNVSKELMLCYLIKPSTMTAEDMETPECMKRIQVQEVILSRWVSSRERSDQDEL 460

BLAST of Pay0009178 vs. ExPASy Swiss-Prot
Match: Q5M954 (tRNA-splicing endonuclease subunit Sen2 OS=Rattus norvegicus OX=10116 GN=Tsen2 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.9e-17
Identity = 51/174 (29.31%), Postives = 86/174 (49.43%), Query Frame = 0

Query: 112 QWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKN 171
           ++ Q  +EEAF+L+Y L CL I   ++  +   +LW+     + TF   Y+AY + R K 
Sbjct: 293 EYLQLSLEEAFFLAYALGCLSIYYEKE-PLTIVKLWQAFTAVQPTFRTTYMAYHYFRSKG 352

Query: 172 WVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLR---VWSDFLCTIRLC 231
           WV + G +YG D + YR  P   H+ ++V++  E  D N  G LR    W       R+ 
Sbjct: 353 WVPKVGLKYGTDLLLYRKGPPFYHASYSVII--ELVDDNFEGSLRRPFSWKSLAALSRVS 412

Query: 232 GSVAKTL----LVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           G+V+K L    L+        +  +P C+    ++E  ++RW   + R +  E+
Sbjct: 413 GNVSKELMLCYLIKPSTMTNEDMETPECMRRIQVQEVILSRWVSSRERSDQDEL 463

BLAST of Pay0009178 vs. ExPASy TrEMBL
Match: A0A1S3CK86 (tRNA-intron lyase OS=Cucumis melo OX=3656 GN=LOC103501916 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.0e-159
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0

Query: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60
           MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS
Sbjct: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60

Query: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120
           TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE
Sbjct: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120

Query: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180
           AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY
Sbjct: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180

Query: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV 240
           GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV
Sbjct: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV 240

Query: 241 NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV
Sbjct: 241 NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 278

BLAST of Pay0009178 vs. ExPASy TrEMBL
Match: A0A0A0KEN4 (tRNA-intron lyase OS=Cucumis sativus OX=3659 GN=Csa_6G290260 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.0e-138
Identity = 245/271 (90.41%), Postives = 252/271 (92.99%), Query Frame = 0

Query: 8   FSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISSTVLHLQS 67
           F   L  P     F LLS V AVDFTVSPPASMAPRWKGKASEAKALADPIS+T+LHLQS
Sbjct: 11  FFIQLTLPAAAHFFFLLSGVAAVDFTVSPPASMAPRWKGKASEAKALADPISNTLLHLQS 70

Query: 68  SFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEEAFYLSYH 127
           SFI SNA+GLLSGS ILYLADEEQTDLLNRTCFGR ILTAEKDKQWFQFGMEEAFYLSYH
Sbjct: 71  SFIHSNAQGLLSGSIILYLADEEQTDLLNRTCFGRPILTAEKDKQWFQFGMEEAFYLSYH 130

Query: 128 LNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAY 187
           LNCLKIVDGQDCE NFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAY
Sbjct: 131 LNCLKIVDGQDCEKNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAY 190

Query: 188 RHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSVNDNGSEF 247
           RHHP+LVHSEFAVLVL EGRDSNGNGRLRVWSDFLCT+RLCGSVAKTLLVL+VNDNGS F
Sbjct: 191 RHHPSLVHSEFAVLVLCEGRDSNGNGRLRVWSDFLCTVRLCGSVAKTLLVLTVNDNGSGF 250

Query: 248 VSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           VSPSCLDSYSIEERTVTRWSPEQCRENPA+V
Sbjct: 251 VSPSCLDSYSIEERTVTRWSPEQCRENPAKV 281

BLAST of Pay0009178 vs. ExPASy TrEMBL
Match: A0A5A7SVN0 (tRNA-intron lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00650 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 9.4e-137
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC
Sbjct: 1   MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 159
           FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE
Sbjct: 61  FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 120

Query: 160 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 219
           FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS
Sbjct: 121 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 180

Query: 220 DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV
Sbjct: 181 DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 239

BLAST of Pay0009178 vs. ExPASy TrEMBL
Match: A0A1S3CKR5 (tRNA-intron lyase OS=Cucumis melo OX=3656 GN=LOC103501916 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.9e-130
Identity = 240/278 (86.33%), Postives = 240/278 (86.33%), Query Frame = 0

Query: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60
           MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS
Sbjct: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60

Query: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120
           TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE
Sbjct: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120

Query: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180
           AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY
Sbjct: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180

Query: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV 240
           GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRV                       
Sbjct: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRV----------------------- 240

Query: 241 NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
                          YSIEERTVTRWSPEQCRENPAEV
Sbjct: 241 --------------CYSIEERTVTRWSPEQCRENPAEV 241

BLAST of Pay0009178 vs. ExPASy TrEMBL
Match: A0A6J1FWV5 (tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111448962 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.3e-114
Identity = 203/239 (84.94%), Postives = 218/239 (91.21%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           M PRWKGKASEA+ALADP+S  VL LQSS  QS+A+GLLSG +ILY ADEEQTDLLNR C
Sbjct: 44  MGPRWKGKASEARALADPMSKIVLQLQSSLTQSDAQGLLSGCSILYQADEEQTDLLNRAC 103

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 159
           FGR I+TAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQD E N QELW+ MK++KATFPE
Sbjct: 104 FGRTIITAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDREKNVQELWQDMKSRKATFPE 163

Query: 160 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 219
           FY+AY+HLR+KNW+VRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG  SN N RLRVWS
Sbjct: 164 FYMAYQHLRMKNWIVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENVRLRVWS 223

Query: 220 DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           DFLCTIRLCGSVAKTLLVLSVNDNGS  VSPSCLD+YSIEERTVTRWSPEQCRENP +V
Sbjct: 224 DFLCTIRLCGSVAKTLLVLSVNDNGSGSVSPSCLDNYSIEERTVTRWSPEQCRENPEKV 282

BLAST of Pay0009178 vs. NCBI nr
Match: XP_008463899.1 (PREDICTED: tRNA-splicing endonuclease subunit Sen2-1 isoform X1 [Cucumis melo])

HSP 1 Score: 570.9 bits (1470), Expect = 6.2e-159
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0

Query: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60
           MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS
Sbjct: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60

Query: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120
           TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE
Sbjct: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120

Query: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180
           AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY
Sbjct: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180

Query: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV 240
           GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV
Sbjct: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV 240

Query: 241 NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV
Sbjct: 241 NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 278

BLAST of Pay0009178 vs. NCBI nr
Match: XP_004149565.1 (tRNA-splicing endonuclease subunit Sen2-1 [Cucumis sativus] >KGN47294.1 hypothetical protein Csa_023042 [Cucumis sativus])

HSP 1 Score: 502.7 bits (1293), Expect = 2.1e-138
Identity = 245/271 (90.41%), Postives = 252/271 (92.99%), Query Frame = 0

Query: 8   FSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISSTVLHLQS 67
           F   L  P     F LLS V AVDFTVSPPASMAPRWKGKASEAKALADPIS+T+LHLQS
Sbjct: 11  FFIQLTLPAAAHFFFLLSGVAAVDFTVSPPASMAPRWKGKASEAKALADPISNTLLHLQS 70

Query: 68  SFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEEAFYLSYH 127
           SFI SNA+GLLSGS ILYLADEEQTDLLNRTCFGR ILTAEKDKQWFQFGMEEAFYLSYH
Sbjct: 71  SFIHSNAQGLLSGSIILYLADEEQTDLLNRTCFGRPILTAEKDKQWFQFGMEEAFYLSYH 130

Query: 128 LNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAY 187
           LNCLKIVDGQDCE NFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAY
Sbjct: 131 LNCLKIVDGQDCEKNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQYGVDFVAY 190

Query: 188 RHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSVNDNGSEF 247
           RHHP+LVHSEFAVLVL EGRDSNGNGRLRVWSDFLCT+RLCGSVAKTLLVL+VNDNGS F
Sbjct: 191 RHHPSLVHSEFAVLVLCEGRDSNGNGRLRVWSDFLCTVRLCGSVAKTLLVLTVNDNGSGF 250

Query: 248 VSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           VSPSCLDSYSIEERTVTRWSPEQCRENPA+V
Sbjct: 251 VSPSCLDSYSIEERTVTRWSPEQCRENPAKV 281

BLAST of Pay0009178 vs. NCBI nr
Match: KAA0035302.1 (tRNA-splicing endonuclease subunit Sen2-1 isoform X1 [Cucumis melo var. makuwa] >TYK14334.1 tRNA-splicing endonuclease subunit Sen2-1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 496.1 bits (1276), Expect = 1.9e-136
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC
Sbjct: 1   MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 159
           FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE
Sbjct: 61  FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 120

Query: 160 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 219
           FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS
Sbjct: 121 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 180

Query: 220 DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV
Sbjct: 181 DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 239

BLAST of Pay0009178 vs. NCBI nr
Match: XP_008463900.1 (PREDICTED: tRNA-splicing endonuclease subunit Sen2-1 isoform X2 [Cucumis melo])

HSP 1 Score: 474.6 bits (1220), Expect = 6.0e-130
Identity = 240/278 (86.33%), Postives = 240/278 (86.33%), Query Frame = 0

Query: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60
           MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS
Sbjct: 1   MISNYFPFSFNLLSPPPHSSFSLLSRVVAVDFTVSPPASMAPRWKGKASEAKALADPISS 60

Query: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120
           TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE
Sbjct: 61  TVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTCFGRQILTAEKDKQWFQFGMEE 120

Query: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180
           AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY
Sbjct: 121 AFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPEFYIAYRHLRLKNWVVRPGSQY 180

Query: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWSDFLCTIRLCGSVAKTLLVLSV 240
           GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRV                       
Sbjct: 181 GVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRV----------------------- 240

Query: 241 NDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
                          YSIEERTVTRWSPEQCRENPAEV
Sbjct: 241 --------------CYSIEERTVTRWSPEQCRENPAEV 241

BLAST of Pay0009178 vs. NCBI nr
Match: XP_038900547.1 (tRNA-splicing endonuclease subunit Sen2-1 [Benincasa hispida] >XP_038900548.1 tRNA-splicing endonuclease subunit Sen2-1 [Benincasa hispida] >XP_038900549.1 tRNA-splicing endonuclease subunit Sen2-1 [Benincasa hispida] >XP_038900550.1 tRNA-splicing endonuclease subunit Sen2-1 [Benincasa hispida] >XP_038900551.1 tRNA-splicing endonuclease subunit Sen2-1 [Benincasa hispida])

HSP 1 Score: 439.5 bits (1129), Expect = 2.2e-119
Identity = 211/239 (88.28%), Postives = 221/239 (92.47%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWKGK SEAKALADP+S+ VL LQSS  QSNA+GLLSGS +LY ADEEQTDLLNR C
Sbjct: 1   MAPRWKGKGSEAKALADPMSNIVLQLQSSLTQSNAQGLLSGSAVLYQADEEQTDLLNRAC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 159
           FGR ILTAEKDKQWFQFGMEEAFYL YHLNCLKIVDGQDCE N QELW+ MK+QK TFPE
Sbjct: 61  FGRPILTAEKDKQWFQFGMEEAFYLLYHLNCLKIVDGQDCEKNVQELWQDMKSQKPTFPE 120

Query: 160 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 219
           FYIAY+HLRLKNWVVRPGSQYGVDFVAYRHHPALVHSE+AVLVLSEG  SN NGRLRVWS
Sbjct: 121 FYIAYQHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEYAVLVLSEGGGSNENGRLRVWS 180

Query: 220 DFLCTIRLCGSVAKTLLVLSVNDNGSEFVSPSCLDSYSIEERTVTRWSPEQCRENPAEV 279
           DFLCTIRLCGSVAKTLLVL+VNDNGS FVSPSCLDSYSIEERTVTRWSPEQCRENPA+V
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGFVSPSCLDSYSIEERTVTRWSPEQCRENPAKV 239

BLAST of Pay0009178 vs. TAIR 10
Match: AT5G60230.2 (splicing endonuclease 2 )

HSP 1 Score: 258.5 bits (659), Expect = 6.3e-69
Identity = 129/238 (54.20%), Postives = 165/238 (69.33%), Query Frame = 0

Query: 39  SMAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRT 98
           SMAPRWK K +EAKALA+PIS +V  LQ S  ++ + G LS   +L   + EQ +LL+R 
Sbjct: 5   SMAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRC 64

Query: 99  CFGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFP 158
           CFGR +L+AEK K+W Q   EEAF+L Y+L C+KI     C  N  + W YMK+++  FP
Sbjct: 65  CFGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFP 124

Query: 159 EFYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVW 218
            F+ AY HLR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV S   D     RLRVW
Sbjct: 125 MFFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLVQSGDSD-----RLRVW 184

Query: 219 SDFLCTIRLCGSVAKTLLVLSVNDN--GSEFVSPSCLDSYSIEERTVTRWSPEQCREN 275
           SD  C +RL GSVAKTLL L VN N  G +     CL+++++EE+T++RWSPE  RE+
Sbjct: 185 SDIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 237

BLAST of Pay0009178 vs. TAIR 10
Match: AT3G45590.1 (splicing endonuclease 1 )

HSP 1 Score: 257.7 bits (657), Expect = 1.1e-68
Identity = 132/238 (55.46%), Postives = 165/238 (69.33%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWK K +EAKALA+P+S TV  L+SS  Q+ A G LS   +L   + E+ +LL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKI-VDGQDCEMNFQELWRYMKTQKATFP 159
           FGR ++ AEKDK+W Q   EEAF+L Y L C+KI + G+  E N  +LWR M + K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 160 EFYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVW 219
             Y AY HLR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S G    GN RL+VW
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIG----GNDRLKVW 180

Query: 220 SDFLCTIRLCGSVAKTLLVLSVND--NGSEFVSPSCLDSYSIEERTVTRWSPEQCREN 275
           SD  C++RL GSVAK+LLVL VN   N  +   P CL+ Y++EE+T+ RWSPE  RE+
Sbjct: 181 SDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSRED 233

BLAST of Pay0009178 vs. TAIR 10
Match: AT3G45590.2 (splicing endonuclease 1 )

HSP 1 Score: 257.7 bits (657), Expect = 1.1e-68
Identity = 132/238 (55.46%), Postives = 165/238 (69.33%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWK K +EAKALA+P+S TV  L+SS  Q+ A G LS   +L   + E+ +LL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKI-VDGQDCEMNFQELWRYMKTQKATFP 159
           FGR ++ AEKDK+W Q   EEAF+L Y L C+KI + G+  E N  +LWR M + K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 160 EFYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVW 219
             Y AY HLR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S G    GN RL+VW
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIG----GNDRLKVW 180

Query: 220 SDFLCTIRLCGSVAKTLLVLSVND--NGSEFVSPSCLDSYSIEERTVTRWSPEQCREN 275
           SD  C++RL GSVAK+LLVL VN   N  +   P CL+ Y++EE+T+ RWSPE  RE+
Sbjct: 181 SDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSRED 233

BLAST of Pay0009178 vs. TAIR 10
Match: AT5G60230.1 (splicing endonuclease 2 )

HSP 1 Score: 256.9 bits (655), Expect = 1.8e-68
Identity = 128/237 (54.01%), Postives = 164/237 (69.20%), Query Frame = 0

Query: 40  MAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRTC 99
           MAPRWK K +EAKALA+PIS +V  LQ S  ++ + G LS   +L   + EQ +LL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 60

Query: 100 FGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFPE 159
           FGR +L+AEK K+W Q   EEAF+L Y+L C+KI     C  N  + W YMK+++  FP 
Sbjct: 61  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 120

Query: 160 FYIAYRHLRLKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGRDSNGNGRLRVWS 219
           F+ AY HLR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV S   D     RLRVWS
Sbjct: 121 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLVQSGDSD-----RLRVWS 180

Query: 220 DFLCTIRLCGSVAKTLLVLSVNDN--GSEFVSPSCLDSYSIEERTVTRWSPEQCREN 275
           D  C +RL GSVAKTLL L VN N  G +     CL+++++EE+T++RWSPE  RE+
Sbjct: 181 DIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 232

BLAST of Pay0009178 vs. TAIR 10
Match: AT3G45577.1 (tRNA-intron endonucleases )

HSP 1 Score: 84.0 bits (206), Expect = 2.1e-16
Identity = 51/133 (38.35%), Postives = 63/133 (47.37%), Query Frame = 0

Query: 39  SMAPRWKGKASEAKALADPISSTVLHLQSSFIQSNARGLLSGSTILYLADEEQTDLLNRT 98
           SMAPRWK                            A G LS   +      EQ +LL+R 
Sbjct: 16  SMAPRWKW---------------------------ALGFLSSCNVFL---SEQAELLDRY 75

Query: 99  CFGRQILTAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEMNFQELWRYMKTQKATFP 158
           CFGR +  AEKDK+W Q   EEAF+L Y L C+KI       +N  +LWR +++ K  F 
Sbjct: 76  CFGRLVFGAEKDKRWIQLSYEEAFFLFYKLKCIKISLHGRSLVNGVDLWRSIRSFKPNFT 118

Query: 159 EFYIAYRHLRLKN 172
             Y AY HLR KN
Sbjct: 136 ILYKAYSHLRSKN 118

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M1E81.5e-6755.46tRNA-splicing endonuclease subunit Sen2-1 OS=Arabidopsis thaliana OX=3702 GN=SEN... [more]
Q9LSS32.6e-6754.01tRNA-splicing endonuclease subunit Sen2-2 OS=Arabidopsis thaliana OX=3702 GN=SEN... [more]
Q5Z6B16.2e-6151.02Probable tRNA-splicing endonuclease subunit Sen2 OS=Oryza sativa subsp. japonica... [more]
Q6P7W51.1e-1729.31tRNA-splicing endonuclease subunit Sen2 OS=Mus musculus OX=10090 GN=Tsen2 PE=1 S... [more]
Q5M9541.9e-1729.31tRNA-splicing endonuclease subunit Sen2 OS=Rattus norvegicus OX=10116 GN=Tsen2 P... [more]
Match NameE-valueIdentityDescription
A0A1S3CK863.0e-159100.00tRNA-intron lyase OS=Cucumis melo OX=3656 GN=LOC103501916 PE=3 SV=1[more]
A0A0A0KEN41.0e-13890.41tRNA-intron lyase OS=Cucumis sativus OX=3659 GN=Csa_6G290260 PE=3 SV=1[more]
A0A5A7SVN09.4e-137100.00tRNA-intron lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G006... [more]
A0A1S3CKR52.9e-13086.33tRNA-intron lyase OS=Cucumis melo OX=3656 GN=LOC103501916 PE=3 SV=1[more]
A0A6J1FWV51.3e-11484.94tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111448962 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008463899.16.2e-159100.00PREDICTED: tRNA-splicing endonuclease subunit Sen2-1 isoform X1 [Cucumis melo][more]
XP_004149565.12.1e-13890.41tRNA-splicing endonuclease subunit Sen2-1 [Cucumis sativus] >KGN47294.1 hypothet... [more]
KAA0035302.11.9e-136100.00tRNA-splicing endonuclease subunit Sen2-1 isoform X1 [Cucumis melo var. makuwa] ... [more]
XP_008463900.16.0e-13086.33PREDICTED: tRNA-splicing endonuclease subunit Sen2-1 isoform X2 [Cucumis melo][more]
XP_038900547.12.2e-11988.28tRNA-splicing endonuclease subunit Sen2-1 [Benincasa hispida] >XP_038900548.1 tR... [more]
Match NameE-valueIdentityDescription
AT5G60230.26.3e-6954.20splicing endonuclease 2 [more]
AT3G45590.11.1e-6855.46splicing endonuclease 1 [more]
AT3G45590.21.1e-6855.46splicing endonuclease 1 [more]
AT5G60230.11.8e-6854.01splicing endonuclease 2 [more]
AT3G45577.12.1e-1638.35tRNA-intron endonucleases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006678tRNA intron endonuclease, N-terminalPFAMPF02778tRNA_int_endo_Ncoord: 75..147
e-value: 1.1E-11
score: 44.2
IPR006677tRNA intron endonuclease, catalytic domain-likePFAMPF01974tRNA_int_endocoord: 161..244
e-value: 9.7E-21
score: 73.6
IPR011856tRNA endonuclease-like domain superfamilyGENE3D3.40.1350.10coord: 156..253
e-value: 5.4E-24
score: 86.0
NoneNo IPR availablePANTHERPTHR21227:SF2TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2-1-LIKEcoord: 40..275
IPR006676tRNA-splicing endonucleasePANTHERPTHR21227TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2coord: 40..275
IPR036167tRNA intron endonuclease, catalytic domain-like superfamilySUPERFAMILY53032tRNA-intron endonuclease catalytic domain-likecoord: 157..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0009178.1Pay0009178.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0000379 tRNA-type intron splice site recognition and cleavage
biological_process GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation
cellular_component GO:0000214 tRNA-intron endonuclease complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000213 tRNA-intron endonuclease activity
molecular_function GO:0004518 nuclease activity