Pay0008534 (gene) Melon (Payzawat) v1

Overview


Name	Pay0008534
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	(R)-specific enoyl-CoA hydratase
Location	chr11: 26727404 .. 26729106 (-)
RNA-Seq Expression	Pay0008534
Synteny	Pay0008534

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTATCTTCCTCTCCTTTCTCTCAGTTTAGACCCATTTATATTCTTCAAGAGGACAGAATCTCGACATTTTCTCAGAATCGTGTAATTAAAGGTAATCTGCTTTCTCTCACTTCCCAAATCCCTTAATTGTTCCTTTCAATTCTCCATTTTTTAAGCAGCGTCTAAAATTTGCCCTGAAGGTGTTTGATGAAATGCCATATTCGAATTTCAATGACTTAGTGAAGCATTCTTGTAGTAGTTACACCCTACAATTAAAATATGATATTTTTATATAGATTATCCAATCATAGAGGTGTTAATGTCGGCATGACCCACTCTTAAAAACCAATGAAAAACTAACTGTTTGCTTAAATCATAGAGCACATACCCGTTTGGAACTTAATATCACTTTAAAAATGTCTCATCCATTGGAAAATAGTCGTAGCCATAATGATTCATGTCGATTACAATTTTCATTTTTAATTTCTGGTTCACAAATTGGGCTTGTAGATACTCTCCCAAGTCTCACCACAATATTTAAATTGTGCCTGAACAAGCATCGAGTTAGGGCTATAACTATTTCGTGAAGGGTGAGATATCTCTAATGCGATATTGAGTTCTGAATAGTTACGTACTCTATAATGTAAGCAAAATGTCTATTTTCATTGGTCATTTTAATGGTGTTTCACTCTGCTTTAACGTCACTGTGATTGAGTCATTCGTACAAAAATCTCTATGAAACAAAAACTTTTTGAGACGAATCCTTTTGGGTTTTTTTATGTAATATTTTAGTTACTTGTTTGATATGAGCGATTTAATTACAAAAGAATGATGCATCCCAGAATGCACTTAAGTATGACATTTTTAGGTGCTTCCATATTTTTCTTCTCTCTACAGCTTTTAATGTTTGCCCCTTTGTTTTCTTGATACAGCTTTGTAGCAAATATTTAAAAGTCCTTTATTTACAAGAATGCTAAGTAGAGGTTTATTCTCAACACATATTTCTTCTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCTATAACAGAATCTTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCTAATCCTCTGCATTTCGATCCCGAATTAGCTCGATGTGCTGGATTCAACGGTTGTCTTGTTCATGGTTTGCTTGTTGCTTCGATGTTCCCTCATATCATTTCCTCTCATTATGTAAGTCTACCCCGTTTGCATTTTCTTACACTTAGTAGAACTTTGGGAGGGGACTGAGAAATGACACCTGAAACGTTCTTACAACTGATTTAATGCTTCTTATCTTTTAATGTATGGTGATGGTCTAATCTTTGAAGATGCTCTGATTTATAATGTTGAGGATGATTGTAGCCCGGAGCCATCTACGTGTCGCAAAGTTTAAATTTTAAGTTGCCGGTTTATGTCGGAGAGAAGATTGTTGGTCAAGTAGAAGCAATCGAGTTGAGAGAAAATAAGAAGAGATACCTGTAAGCTTGTTCTTGGGCGAATCTCTACCTTTTCAAATTCAAATTTCAAGTTATCATTTCATCGGGTTTGAGAACGCCAAATGTCTTTCAGGGCTAAATTCAAAACAAAATGTTTGAGGAATGGTGACGAGCTTGTTCTCGAAGGCGAGGCAAGGGCCATCTTACCGGCCTATTTTCATTCCACAAACGAGTAG

mRNA sequence

ATGTTATCTTCCTCTCCTTTCTCTCAGTTTAGACCCATTTATATTCTTCAAGAGGACAGAATCTCGACATTTTCTCAGAATCGTCAAATATTTAAAAGTCCTTTATTTACAAGAATGCTAAGTAGAGGTTTATTCTCAACACATATTTCTTCTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCTATAACAGAATCTTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCTAATCCTCTGCATTTCGATCCCGAATTAGCTCGATGTGCTGGATTCAACGGTTGTCTTGTTCATGGTTTGCTTGTTGCTTCGATGTTCCCTCATATCATTTCCTCTCATTATCCCGGAGCCATCTACGTGTCGCAAAGTTTAAATTTTAAGTTGCCGGTTTATGTCGGAGAGAAGATTGTTGGTCAAGTAGAAGCAATCGAGTTGAGAGAAAATAAGAAGAGATACCTGGCTAAATTCAAAACAAAATGTTTGAGGAATGGTGACGAGCTTGTTCTCGAAGGCGAGGCAAGGGCCATCTTACCGGCCTATTTTCATTCCACAAACGAGTAG

Coding sequence (CDS)

ATGTTATCTTCCTCTCCTTTCTCTCAGTTTAGACCCATTTATATTCTTCAAGAGGACAGAATCTCGACATTTTCTCAGAATCGTCAAATATTTAAAAGTCCTTTATTTACAAGAATGCTAAGTAGAGGTTTATTCTCAACACATATTTCTTCTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCTATAACAGAATCTTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCTAATCCTCTGCATTTCGATCCCGAATTAGCTCGATGTGCTGGATTCAACGGTTGTCTTGTTCATGGTTTGCTTGTTGCTTCGATGTTCCCTCATATCATTTCCTCTCATTATCCCGGAGCCATCTACGTGTCGCAAAGTTTAAATTTTAAGTTGCCGGTTTATGTCGGAGAGAAGATTGTTGGTCAAGTAGAAGCAATCGAGTTGAGAGAAAATAAGAAGAGATACCTGGCTAAATTCAAAACAAAATGTTTGAGGAATGGTGACGAGCTTGTTCTCGAAGGCGAGGCAAGGGCCATCTTACCGGCCTATTTTCATTCCACAAACGAGTAG

Protein sequence

MLSSSPFSQFRPIYILQEDRISTFSQNRQIFKSPLFTRMLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE

Homology

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BLAST of Pay0008534 vs. ExPASy Swiss-Prot
Match: O32472 ((R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.5e-19
Identity = 51/131 (38.93%), Postives = 77/131 (58.78%), Query Frame = 0


Query: 64  LKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGLLVASMFPHI 123
           L+VG     ++ F + +V  ++ +S D NPLH DP  A    F   +VHG+L+AS+F  +
Sbjct: 6   LEVGQKARLSKRFGAAEVAAFAALSEDFNPLHLDPAFAATTAFERPIVHGMLLASLFSGL 65

Query: 124 ISSHYP--GAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKCLRNGDEL 183
           +    P  G+IY+ QSL+FKLPV+VG+++  +VE   LRE+K   +A   T+    G  L
Sbjct: 66  LGQQLPGKGSIYLGQSLSFKLPVFVGDEVTAEVEVTALREDKP--IATLTTRIFTQGGAL 125

Query: 184 VLEGEARAILP 193
            + GEA   LP
Sbjct: 126 AVTGEAVVKLP 134

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BLAST of Pay0008534 vs. ExPASy Swiss-Prot
Match: P86397 (Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 GN=HTD2 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-14
Identity = 40/132 (30.30%), Postives = 73/132 (55.30%), Query Frame = 0


Query: 64  LKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGLLVASMFPHI 123
           +KVGD     R FT  DV  +S+++ D NPLH + + A+   F   +VHG+L+  +   +
Sbjct: 36  IKVGDRAELRRAFTQTDVATFSELTGDVNPLHLNEDFAKHTKFGNTIVHGVLINGLISAL 95

Query: 124 ISSHY--PGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKC-LRNGDE 183
           + +    PG +++SQ ++F  P+Y+GE ++   E  +L    KR++A     C +    +
Sbjct: 96  LGTKMPGPGCVFLSQEISFPAPLYIGEVVLASAEVKKL----KRFIAIIAVSCSVIESKK 155

Query: 184 LVLEGEARAILP 193
            V+EG  + ++P
Sbjct: 156 TVMEGWVKVMVP 163

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BLAST of Pay0008534 vs. ExPASy Swiss-Prot
Match: C5AZ74 (3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) OX=272630 GN=croR PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-13
Identity = 44/131 (33.59%), Postives = 73/131 (55.73%), Query Frame = 0


Query: 64  LKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGLLVASMFPHI 123
           L+VG      +  +S DV+ +++++ D NP+H     A    F   + HGL  A +   +
Sbjct: 13  LEVGLTEILKKEISSSDVVGFAEITGDRNPIHLSEHFAARTPFGTRIAHGLYTAGLISAV 72

Query: 124 ISSHY--PGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKCLRNGDEL 183
           + +    PGA+Y+SQ+LNF+ PV +G+ +  +VE  EL   ++R  A+    C   GDE+
Sbjct: 73  LGTRLPGPGAVYISQTLNFRAPVRIGDIVEVKVEVAELIPERRR--ARLACTC-SVGDEV 132

Query: 184 VLEGEARAILP 193
           VL+GEA   +P
Sbjct: 133 VLDGEALVKVP 140

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BLAST of Pay0008534 vs. ExPASy Swiss-Prot
Match: Q2RQ36 ((R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=phaJ PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.7e-13
Identity = 44/132 (33.33%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 64  LKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGLLVASMFPHI 123
           +K G   S  +  +  D+  ++ +S D+NP H + + A+   F   + HG+L A     +
Sbjct: 14  IKEGQSASLAKTISESDIYLFAGLSMDTNPAHVNEDYAQTTVFKTRIAHGMLSAGFISAV 73

Query: 124 ISSHY--PGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKCLRNGDEL 183
           + +    PGAIYV+QSL FK PV +G+ +   V    L   KK     F+T C   G ++
Sbjct: 74  LGTRLPGPGAIYVNQSLKFKAPVRIGDTVTATVTVTGLVPEKK--FVTFRTTCTVAG-KV 133

Query: 184 VLEGEARAILPA 194
           V+EGEA  ++PA
Sbjct: 134 VIEGEATVMVPA 142

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BLAST of Pay0008534 vs. ExPASy Swiss-Prot
Match: Q9VXJ0 (Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=Mfe2 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 5.9e-05
Identity = 32/109 (29.36%), Postives = 52/109 (47.71%), Query Frame = 0


Query: 77  TSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGLLVASMFPHIISSHYPG---AIY 136
           TSED     ++S D NPLH DP++A  AGF   ++HGL         + + +     A++
Sbjct: 483 TSEDQAALYRLSGDKNPLHIDPQMALLAGFKTPILHGLCTLGFSVRAVLAQFADNNPALF 542

Query: 137 VSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKCLRNGDELV 183
            +  + F  PV     I GQ   ++L +   R    F+T  +  G E++
Sbjct: 543 KAVKVRFSGPV-----IPGQTLRVDLWKQGTR--INFRTVVVETGKEVI 584

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BLAST of Pay0008534 vs. ExPASy TrEMBL
Match: A0A1S3CLU3 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.6e-104
Identity = 199/206 (96.60%), Postives = 199/206 (96.60%), Query Frame = 0


Query: 1   MLSSSPFSQFRPIYILQEDRISTFSQNR------QIFKSPLFTRMLSRGLFSTHISSSLK 60
           MLSSSPFSQFRPIYILQEDRISTFSQNR      QIFKSPLFTRMLSRGLFSTHISSSLK
Sbjct: 1   MLSSSPFSQFRPIYILQEDRISTFSQNRVIKALQQIFKSPLFTRMLSRGLFSTHISSSLK 60

Query: 61  CFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGL 120
           CFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELAR AGFNGCLVHGL
Sbjct: 61  CFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARRAGFNGCLVHGL 120

Query: 121 LVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKC 180
           LVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKC
Sbjct: 121 LVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKC 180

Query: 181 LRNGDELVLEGEARAILPAYFHSTNE 201
           LRNGDELVLEGEARAILPAYFHSTNE
Sbjct: 181 LRNGDELVLEGEARAILPAYFHSTNE 206

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BLAST of Pay0008534 vs. ExPASy TrEMBL
Match: A0A5A7T147 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00540 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.7e-84
Identity = 161/162 (99.38%), Postives = 161/162 (99.38%), Query Frame = 0


Query: 39  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 98
           MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP
Sbjct: 1   MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 60

Query: 99  ELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 158
           ELAR AGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE
Sbjct: 61  ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 159 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE 201
           LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE 162

[Close]

BLAST of Pay0008534 vs. ExPASy TrEMBL
Match: A0A0A0KCN3 (MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.2e-79
Identity = 153/162 (94.44%), Postives = 158/162 (97.53%), Query Frame = 0


Query: 39  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 98
           MLS+GLFST+ISSSLKCFSSSTS+VLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 
Sbjct: 1   MLSKGLFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDS 60

Query: 99  ELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 158
           +LA+ AGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE
Sbjct: 61  KLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 159 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE 201
           LRENKKRYLAKFKTKCLRNGDELVLEGEARAILP  FHSTNE
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTNE 162

[Close]

BLAST of Pay0008534 vs. ExPASy TrEMBL
Match: A0A6J1JDU3 (uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.4e-65
Identity = 134/162 (82.72%), Postives = 148/162 (91.36%), Query Frame = 0


Query: 39  MLSR-GLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 98
           MLSR GL S++ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 99  PELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 158
             LAR AGF+  LVHG+LVASMFP +ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 159 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 200
           +LRE KKRYLAKF+TKCLRNGDELVL+GEARAILP+ F ST+
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTD 162

[Close]

BLAST of Pay0008534 vs. ExPASy TrEMBL
Match: A0A6J1FRZ2 (uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC111448279 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 7.6e-64
Identity = 131/156 (83.97%), Postives = 143/156 (91.67%), Query Frame = 0


Query: 39  MLSR-GLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 98
           MLSR GL S+ ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSCISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 99  PELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 158
             LAR AGF+  LVHG+LVASMFPH+ISSH+PGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 159 ELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPA 194
           +LRE KKRYL KF+TKCLRNGDELVL+GEARAILP+
Sbjct: 121 DLREIKKRYLVKFQTKCLRNGDELVLDGEARAILPS 156

[Close]

BLAST of Pay0008534 vs. NCBI nr
Match: XP_008463911.1 (PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo])

HSP 1 Score: 387.9 bits (995), Expect = 5.4e-104
Identity = 199/206 (96.60%), Postives = 199/206 (96.60%), Query Frame = 0


Query: 1   MLSSSPFSQFRPIYILQEDRISTFSQNR------QIFKSPLFTRMLSRGLFSTHISSSLK 60
           MLSSSPFSQFRPIYILQEDRISTFSQNR      QIFKSPLFTRMLSRGLFSTHISSSLK
Sbjct: 1   MLSSSPFSQFRPIYILQEDRISTFSQNRVIKALQQIFKSPLFTRMLSRGLFSTHISSSLK 60

Query: 61  CFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLVHGL 120
           CFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELAR AGFNGCLVHGL
Sbjct: 61  CFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARRAGFNGCLVHGL 120

Query: 121 LVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKC 180
           LVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKC
Sbjct: 121 LVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFKTKC 180

Query: 181 LRNGDELVLEGEARAILPAYFHSTNE 201
           LRNGDELVLEGEARAILPAYFHSTNE
Sbjct: 181 LRNGDELVLEGEARAILPAYFHSTNE 206

[Close]

BLAST of Pay0008534 vs. NCBI nr
Match: KAA0035311.1 ((R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa])

HSP 1 Score: 322.0 bits (824), Expect = 3.6e-84
Identity = 161/162 (99.38%), Postives = 161/162 (99.38%), Query Frame = 0


Query: 39  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 98
           MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP
Sbjct: 1   MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 60

Query: 99  ELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 158
           ELAR AGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE
Sbjct: 61  ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 159 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE 201
           LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE 162

[Close]

BLAST of Pay0008534 vs. NCBI nr
Match: XP_004149553.1 (uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical protein Csa_022963 [Cucumis sativus])

HSP 1 Score: 305.1 bits (780), Expect = 4.6e-79
Identity = 153/162 (94.44%), Postives = 158/162 (97.53%), Query Frame = 0


Query: 39  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 98
           MLS+GLFST+ISSSLKCFSSSTS+VLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFD 
Sbjct: 1   MLSKGLFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDS 60

Query: 99  ELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 158
           +LA+ AGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE
Sbjct: 61  KLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 159 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTNE 201
           LRENKKRYLAKFKTKCLRNGDELVLEGEARAILP  FHSTNE
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTNE 162

[Close]

BLAST of Pay0008534 vs. NCBI nr
Match: XP_038901209.1 ((R)-specific enoyl-CoA hydratase [Benincasa hispida])

HSP 1 Score: 286.2 bits (731), Expect = 2.2e-73
Identity = 143/161 (88.82%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 39  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 98
           MLSRGLFS HIS+SLKCFSSSTSNVLKVGDILSY RIFTS+DVLEYSKVSHDSNPLHFD 
Sbjct: 1   MLSRGLFSMHISTSLKCFSSSTSNVLKVGDILSYTRIFTSKDVLEYSKVSHDSNPLHFDS 60

Query: 99  ELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 158
           ELAR AGFNG LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGE IVGQVEAI+
Sbjct: 61  ELARRAGFNGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGENIVGQVEAID 120

Query: 159 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 200
           LRENKKRYLAKF TKC+RNG E+VLEGEARAI+P+ FHSTN
Sbjct: 121 LRENKKRYLAKFITKCMRNGHEVVLEGEARAIIPSDFHSTN 161

[Close]

BLAST of Pay0008534 vs. NCBI nr
Match: KAG7010611.1 ((R)-specific enoyl-CoA hydratase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.1 bits (671), Expect = 2.0e-66
Identity = 136/165 (82.42%), Postives = 151/165 (91.52%), Query Frame = 0


Query: 36  FTRMLSR-GLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPL 95
           FT MLSR GL S++ISSSLKCFSSSTSNVLKVGDILS+ RIFTSEDVLEYSKVSHDSNPL
Sbjct: 14  FTSMLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPL 73

Query: 96  HFDPELARCAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQV 155
           HFD  LAR AGF+  LVHG+LVASMFPH+ISSH+PGAIYVSQSLNFKLPVYVGE I+ +V
Sbjct: 74  HFDSALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRV 133

Query: 156 EAIELRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 200
           EAI+LRE KKR+LAKF+TKCLRNGDELVL+GEARAILP+ F ST+
Sbjct: 134 EAIDLREIKKRHLAKFQTKCLRNGDELVLDGEARAILPSGFCSTD 178

[Close]

BLAST of Pay0008534 vs. TAIR 10
Match: AT5G60335.1 (Thioesterase superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 2.7e-45
Identity = 87/142 (61.27%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 53  LKCFSS-STSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDPELARCAGFNGCLV 112
           L+ FSS ++  +LKVGD+L   R+F+SED+  Y++VSHD NPLHFDPE AR AGF   LV
Sbjct: 14  LRSFSSVASKTLLKVGDVLRETRVFSSEDIKAYAEVSHDWNPLHFDPESARKAGFENRLV 73

Query: 113 HGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIELRENKKRYLAKFK 172
           HG+LV+SMFP IIS+H+PGA+YVSQSL+F+ PVY+G++I+G V+AI LRE K +Y+ KF 
Sbjct: 74  HGMLVSSMFPRIISAHFPGAVYVSQSLHFRSPVYIGDEILGLVQAIALRETKNKYIVKFS 133

Query: 173 TKCLRNGDEL-VLEGEARAILP 193
           TKC +N +EL V++GEA AILP
Sbjct: 134 TKCFKNHNELVVIDGEATAILP 155

The following BLAST results are available for this feature:

BLAST of Pay0008534 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O32472	2.5e-19	38.93	(R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1	[more]
P86397	2.4e-14	30.30	Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 ...	[more]
C5AZ74	1.2e-13	33.59	3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 ...	[more]
Q2RQ36	2.7e-13	33.33	(R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / A...	[more]
Q9VXJ0	5.9e-05	29.36	Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=...	[more]

BLAST of Pay0008534 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CLU3	2.6e-104	96.60	(R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV...	[more]
A0A5A7T147	1.7e-84	99.38	(R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0KCN3	2.2e-79	94.44	MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 P...	[more]
A0A6J1JDU3	2.4e-65	82.72	uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634...	[more]
A0A6J1FRZ2	7.6e-64	83.97	uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC1114482...	[more]

BLAST of Pay0008534 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008463911.1	5.4e-104	96.60	PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo]	[more]
KAA0035311.1	3.6e-84	99.38	(R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-spec...	[more]
XP_004149553.1	4.6e-79	94.44	uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical ...	[more]
XP_038901209.1	2.2e-73	88.82	(R)-specific enoyl-CoA hydratase [Benincasa hispida]	[more]
KAG7010611.1	2.0e-66	82.42	(R)-specific enoyl-CoA hydratase, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of Pay0008534 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60335.1	2.7e-45	61.27	Thioesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 53..194 e-value: 1.8E-34 score: 120.2
None	No IPR available	PANTHER	PTHR43437	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 45..192
None	No IPR available	PANTHER	PTHR43437:SF3	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 45..192
None	No IPR available	CDD	cd03449	R_hydratase	coord: 64..191 e-value: 1.49628E-46 score: 147.692
IPR002539	MaoC-like dehydratase domain	PFAM	PF01575	MaoC_dehydratas	coord: 76..163 e-value: 8.8E-17 score: 60.8
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 64..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0008534.1	Pay0008534.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0045145	single-stranded DNA 5'-3' exodeoxyribonuclease activity