Pay0005359 (gene) Melon (Payzawat) v1

Overview
NamePay0005359
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionEncodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: (RGF1), (RGF2), (RGF3), (RGF4), (RGF5), (RGF6), (RGF7), (RGF8) and (RGF9).
Locationchr06: 33761089 .. 33762278 (-)
RNA-Seq ExpressionPay0005359
SyntenyPay0005359
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACCTTCTTCTCTTTCACTTTCTCTCTTTCTCCTTTCTCATGGCTATGCCTTGTTTCTTTCTCACTCTTTTATTCTTCTTATCTACGTTGCATGCGTGTGACGCTAGTCGTCTTCGATTCTTCCAGAGCTCGAATTTTGTTTCCAGGATTGAACCGCATTCGATCTCTCAAGGGATTTGGACAGATCATGATCAGGAGAGAAGTTTTTACAAAAACAACAGTGACAAGGTTCTTCCTCATAAGGAGATTATTGTTGGGATTGAAATGTTAAAGGTATACTACATATATATATATATATATATATATATATATATATATATATACTTCATCTCCCATCTTGTGCTTTCTAAAGATGAGCTTGTAATTTAGTTCATAATTACTAGCTAGCTATATGAGAGGGGGAGGGGGAGTTTTCAGATATGTAGACATTTTCATTAACATGTCTACATCTTGATAAAACTCAATATCAAAATATATATGAAGTGATAAAAATATACTTTCCCTCTAAATAATGCAATAAACATGTCAACCAAAGTATATATATATATATGCATACATTATCTTATTTAAAAATTCAATCAAATGGTTTTTGGTCGAAATATAATGTTTATCGATATCAAATATATTTGTTTTCAAAGAAACCAACATATAATATCTTTTTATCATTATTTCTATTTACATTTGTACAAACTTGGAAATCTCATAATTTTTATCGACATCAACATTTAGTTCTCGAGTCAAGACAATAATTATGGTGGATATAGACACCATTTAATTTCTTTGGTACAAAATAATAATATCATTAATATAGACAAAGGCATATGAATGGTAAATTGCATGAAAAAAGGGAATGGATTATTTGGAAATATAGAGAGTGTGTGATTAATGAATTAACTTTGGTATTTTAATTTCTCAGGGAATGAAGAAGGATGGAAGCAGATTGGTAATGGAACTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCATAGAAAGCCTCCTATTCACAATGAAAGACCCTAGATATATATATACATATATGTAATGTAATAGTTCTCAAATTTGCCATCAATATCTAAATATGTTTCAAAAATTAAAGTCTTTTTTTTATATATATATTTTGGAATAAAGAAAAAAGAAT

mRNA sequence

CAAAACCTTCTTCTCTTTCACTTTCTCTCTTTCTCCTTTCTCATGGCTATGCCTTGTTTCTTTCTCACTCTTTTATTCTTCTTATCTACGTTGCATGCGTGTGACGCTAGTCGTCTTCGATTCTTCCAGAGCTCGAATTTTGTTTCCAGGATTGAACCGCATTCGATCTCTCAAGGGATTTGGACAGATCATGATCAGGAGAGAAGTTTTTACAAAAACAACAGTGACAAGGTTCTTCCTCATAAGGAGATTATTGTTGGGATTGAAATGTTAAAGGGAATGAAGAAGGATGGAAGCAGATTGGTAATGGAACTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCATAGAAAGCCTCCTATTCACAATGAAAGACCCTAGATATATATATACATATATGTAATGTAATAGTTCTCAAATTTGCCATCAATATCTAAATATGTTTCAAAAATTAAAGTCTTTTTTTTATATATATATTTTGGAATAAAGAAAAAAGAAT

Coding sequence (CDS)

ATGGCTATGCCTTGTTTCTTTCTCACTCTTTTATTCTTCTTATCTACGTTGCATGCGTGTGACGCTAGTCGTCTTCGATTCTTCCAGAGCTCGAATTTTGTTTCCAGGATTGAACCGCATTCGATCTCTCAAGGGATTTGGACAGATCATGATCAGGAGAGAAGTTTTTACAAAAACAACAGTGACAAGGTTCTTCCTCATAAGGAGATTATTGTTGGGATTGAAATGTTAAAGGGAATGAAGAAGGATGGAAGCAGATTGGTAATGGAACTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCATAGAAAGCCTCCTATTCACAATGAAAGACCCTAG

Protein sequence

MAMPCFFLTLLFFLSTLHACDASRLRFFQSSNFVSRIEPHSISQGIWTDHDQERSFYKNNSDKVLPHKEIIVGIEMLKGMKKDGSRLVMELPMKEKVMNSKENEVGEDMVVMDYAQPHRKPPIHNERP
Homology
BLAST of Pay0005359 vs. ExPASy Swiss-Prot
Match: Q93VK8 (Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-04
Identity = 19/24 (79.17%), Postives = 22/24 (91.67%), Query Frame = 0

Query: 104 EVGEDMVVMDYAQPHRKPPIHNER 128
           E GE++VVMDY QPHRKPPIHNE+
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

BLAST of Pay0005359 vs. ExPASy TrEMBL
Match: A0A0A0L298 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.1e-50
Identity = 109/130 (83.85%), Postives = 118/130 (90.77%), Query Frame = 0

Query: 1   MAMPCFFLTLLFFLSTLHACDASRLRFFQSSNFVSRI-EPHSISQGIWT-DHDQERSFYK 60
           MAMP FFL LLFF STLHACDASRLRFFQSSNFVSRI E HS+SQGIW+ +HDQE SF K
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  NNSDKVLPHKEIIVGIEMLKGMKKDGSRLVMELPMKEKVMNSKENEVGEDMVVMDYAQPH 120
           NN+D +L +KE I+GIEMLKGMK+DGSRLVME PMKEKVMNSKENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNERP 129
           RKPPIHNE+P
Sbjct: 121 RKPPIHNEKP 130

BLAST of Pay0005359 vs. ExPASy TrEMBL
Match: A0A6J1DKL1 (uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021396 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.3e-19
Identity = 77/132 (58.33%), Postives = 87/132 (65.91%), Query Frame = 0

Query: 1   MAMPCFFLTLLFFLSTLHACDA-SRLRFFQSSNFVSRIEPHS-ISQGIWTDHDQERSFYK 60
           MA P     LL  LSTLHACDA  RL+ F+ SN VSR+   + I Q   TD   ERS  K
Sbjct: 1   MATPSLLFILL--LSTLHACDACHRLQLFEYSNPVSRLGLLAPIPQEFRTD-QPERSLNK 60

Query: 61  NNSDKVLPHKEIIVGIEMLKGMKKDGSRLVMELPM--KEKVMNSKENEVGEDMVVMDYAQ 120
           N   KV    EI  GI +LKGMKK   RL ME  M  KEK+M+ +ENEVGEDMVVMDYAQ
Sbjct: 61  NIIHKVSSSVEI--GIGILKGMKKH-KRLAMEFSMKEKEKLMSYEENEVGEDMVVMDYAQ 120

Query: 121 PHRKPPIHNERP 129
           PHRKPPIHN++P
Sbjct: 121 PHRKPPIHNKKP 126

BLAST of Pay0005359 vs. ExPASy TrEMBL
Match: A0A6J1KZM9 (uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 4.8e-19
Identity = 73/139 (52.52%), Postives = 88/139 (63.31%), Query Frame = 0

Query: 1   MAMP-CFFLTLLFFLSTLHACDASRLRFFQSSNFVSRIEPHSISQGIWTDHDQERSFYKN 60
           M MP   FL LLF  + LHACDA  L+   S    SR+E   IS G   D   +RS  K+
Sbjct: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHS----SRLESCMISPGFRMD-QLKRSLDKS 60

Query: 61  NSDKVLPHKEIIVGIEMLKGMKKDGSRLVMELPMK----------EKVMNSKENEVGEDM 120
            S++V   +   VGIE+LKGMKK   RL MELP++          +KV+NS+ENEVGEDM
Sbjct: 61  KSNEVSSVEN--VGIEILKGMKK-LRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDM 120

Query: 121 VVMDYAQPHRKPPIHNERP 129
           VVMDYAQPHRKPPIHN +P
Sbjct: 121 VVMDYAQPHRKPPIHNRKP 131

BLAST of Pay0005359 vs. ExPASy TrEMBL
Match: A0A6S7NRQ9 (Uncharacterized protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS30448 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.6e-06
Identity = 47/131 (35.88%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 16  TLHACDASRLRFFQSSNFVSRIEPHSISQGIWTDHDQERSFYKNNSDK----VLPHKEII 75
           ++HACD+  L  F            S +  I   H+       N  DK      P +E+ 
Sbjct: 15  SMHACDSRHLGVFHKEPGKELHLITSKAPVIRVGHEGRNCKETNMEDKESKLEFPSEELK 74

Query: 76  V--GIEMLKGMKKDG---------SRLVMELPM---KEKVMNSKENEVGEDMVVMDYAQP 129
           +  G ++   + +DG         +RL ME      KEK+ NSK+N V ED+ VMDYAQP
Sbjct: 75  MRKGNDIELNLSQDGTKPQGWKNRARLTMESKQKDSKEKINNSKDNSVTEDVGVMDYAQP 134

BLAST of Pay0005359 vs. ExPASy TrEMBL
Match: A0A6P6TWI6 (uncharacterized protein LOC113705039 OS=Coffea arabica OX=13443 GN=LOC113705039 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 7.9e-06
Identity = 29/46 (63.04%), Postives = 36/46 (78.26%), Query Frame = 0

Query: 85  SRLVMELP--MKEKVMNSKENEVGEDMVVMDYAQPHRKPPIHNERP 129
           +R +ME P    E+ ++S EN+ GED+VVMDYAQPHRKPPIHN RP
Sbjct: 148 ARSIMESPTAKAEETVDSMENDKGEDIVVMDYAQPHRKPPIHNRRP 193

BLAST of Pay0005359 vs. NCBI nr
Match: XP_011650407.1 (uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical protein Csa_010787 [Cucumis sativus])

HSP 1 Score: 209.1 bits (531), Expect = 2.2e-50
Identity = 109/130 (83.85%), Postives = 118/130 (90.77%), Query Frame = 0

Query: 1   MAMPCFFLTLLFFLSTLHACDASRLRFFQSSNFVSRI-EPHSISQGIWT-DHDQERSFYK 60
           MAMP FFL LLFF STLHACDASRLRFFQSSNFVSRI E HS+SQGIW+ +HDQE SF K
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  NNSDKVLPHKEIIVGIEMLKGMKKDGSRLVMELPMKEKVMNSKENEVGEDMVVMDYAQPH 120
           NN+D +L +KE I+GIEMLKGMK+DGSRLVME PMKEKVMNSKENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNERP 129
           RKPPIHNE+P
Sbjct: 121 RKPPIHNEKP 130

BLAST of Pay0005359 vs. NCBI nr
Match: XP_038905997.1 (uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida])

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-45
Identity = 102/126 (80.95%), Postives = 110/126 (87.30%), Query Frame = 0

Query: 3   MPCFFLTLLFFLSTLHACDASRLRFFQSSNFVSRIEPHSISQGIWTDHDQERSFYKNNSD 62
           MP  FL LL F STLHAC+A  LRFF+SS+FVSRIEPHSISQGIW D   ERSF ++NSD
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRIEPHSISQGIWAD-QPERSFEESNSD 60

Query: 63  KVLPHKEIIVGIEMLKGMKKDGSRLVMELPMKEKVMNSKENEVGEDMVVMDYAQPHRKPP 122
           K L +KEIIVGIEMLKGMKK GSRLVME PMKEKV+NSKENEVGEDMVVMDYAQPHRKPP
Sbjct: 61  K-LSYKEIIVGIEMLKGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPHRKPP 120

Query: 123 IHNERP 129
           IHNE+P
Sbjct: 121 IHNEKP 124

BLAST of Pay0005359 vs. NCBI nr
Match: XP_038905996.1 (uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida])

HSP 1 Score: 188.7 bits (478), Expect = 3.1e-44
Identity = 102/127 (80.31%), Postives = 110/127 (86.61%), Query Frame = 0

Query: 3   MPCFFLTLLFFLSTLHACDASRLRFFQSSNFVSRIEPHSISQGIWTDHDQERSFYKNNSD 62
           MP  FL LL F STLHAC+A  LRFF+SS+FVSRIEPHSISQGIW D   ERSF ++NSD
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRIEPHSISQGIWAD-QPERSFEESNSD 60

Query: 63  KVLPHKEIIVGIEMLK-GMKKDGSRLVMELPMKEKVMNSKENEVGEDMVVMDYAQPHRKP 122
           K L +KEIIVGIEMLK GMKK GSRLVME PMKEKV+NSKENEVGEDMVVMDYAQPHRKP
Sbjct: 61  K-LSYKEIIVGIEMLKQGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPHRKP 120

Query: 123 PIHNERP 129
           PIHNE+P
Sbjct: 121 PIHNEKP 125

BLAST of Pay0005359 vs. NCBI nr
Match: KAG7028383.1 (Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 107.8 bits (268), Expect = 6.8e-20
Identity = 73/137 (53.28%), Postives = 91/137 (66.42%), Query Frame = 0

Query: 1   MAMP-CFFLTLLFFLSTLHACDASRLRFFQSSNFVSRIEPHSISQGIWTDHDQERSFYKN 60
           M MP   FL+LLF  + LHACDA  L+   S    SR+EP  IS G  T +  E+S  ++
Sbjct: 1   MPMPTSLFLSLLFLSTFLHACDARLLKLCHS----SRLEPCMISPGFRT-NQLEKSLDES 60

Query: 61  NSDKVLPHKEIIVGIEMLKGMKKDGSRLVMELPMK--------EKVMNSKENEVGEDMVV 120
            S+ V   +   VGIE+LKGMKK   RL MELP++        +KV+NS+ENEVGEDMVV
Sbjct: 61  RSNDVSSMEN--VGIEILKGMKK-LRRLAMELPVRDIDRDKDIDKVVNSEENEVGEDMVV 120

Query: 121 MDYAQPHRKPPIHNERP 129
           MDYAQPHRKPPIHN++P
Sbjct: 121 MDYAQPHRKPPIHNQKP 129

BLAST of Pay0005359 vs. NCBI nr
Match: XP_023540680.1 (uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 107.5 bits (267), Expect = 8.9e-20
Identity = 74/137 (54.01%), Postives = 90/137 (65.69%), Query Frame = 0

Query: 1   MAMP-CFFLTLLFFLSTLHACDASRLRFFQSSNFVSRIEPHSISQGIWTDHDQERSFYKN 60
           M MP  FFL LLF  + LHACDA  L+   S    SR+E   IS G  T +  ERS  ++
Sbjct: 1   MPMPTSFFLALLFLSTFLHACDARLLKLCHS----SRLESCLISPGFRT-NQLERSLDES 60

Query: 61  NSDKVLPHKEIIVGIEMLKGMKKDGSRLVMELPMK--------EKVMNSKENEVGEDMVV 120
            S+ V   +   VGIE+LKGMKK   RL MELP++        +KV+NS+ENEVGEDMVV
Sbjct: 61  KSNDVSSMEN--VGIEILKGMKK-LRRLAMELPVRDRDRDKDIDKVVNSEENEVGEDMVV 120

Query: 121 MDYAQPHRKPPIHNERP 129
           MDYAQPHRKPPIHN++P
Sbjct: 121 MDYAQPHRKPPIHNQKP 129

BLAST of Pay0005359 vs. TAIR 10
Match: AT4G16515.1 (Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: At5g60810 (RGF1), At1g13620 (RGF2), At2g04025 (RGF3), At3g30350 (RGF4), At5g51451 (RGF5), At4g16515 (RGF6), At3g02240 (RGF7), At2g03830 (RGF8) and At5g64770 (RGF9). )

HSP 1 Score: 47.4 bits (111), Expect = 1.0e-05
Identity = 19/24 (79.17%), Postives = 22/24 (91.67%), Query Frame = 0

Query: 104 EVGEDMVVMDYAQPHRKPPIHNER 128
           E GE++VVMDY QPHRKPPIHNE+
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VK81.4e-0479.17Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2981.1e-5083.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1[more]
A0A6J1DKL11.3e-1958.33uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A6J1KZM94.8e-1952.52uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521... [more]
A0A6S7NRQ94.6e-0635.88Uncharacterized protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS30448 PE=4 SV=1[more]
A0A6P6TWI67.9e-0663.04uncharacterized protein LOC113705039 OS=Coffea arabica OX=13443 GN=LOC113705039 ... [more]
Match NameE-valueIdentityDescription
XP_011650407.12.2e-5083.85uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical ... [more]
XP_038905997.11.2e-4580.95uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida][more]
XP_038905996.13.1e-4480.31uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida][more]
KAG7028383.16.8e-2053.28Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023540680.18.9e-2054.01uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G16515.11.0e-0579.17Encodes a root meristem growth factor (RGF). Belongs to a family of functionall... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34961TRANSMEMBRANE PROTEINcoord: 70..128
NoneNo IPR availablePANTHERPTHR34961:SF7SUBFAMILY NOT NAMEDcoord: 70..128

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0005359.1Pay0005359.1mRNA