Pay0005287 (gene) Melon (Payzawat) v1

Overview
NamePay0005287
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr11: 29609919 .. 29612302 (+)
RNA-Seq ExpressionPay0005287
SyntenyPay0005287
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTCAATGGGGTTCTCATTTTAATGATTTGGGTTATAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAGATGGAAGATACCATTTTCATAAGGGTAAAAAAGATGAAGATTCCCCTGTTTCTTCACCACCAGAGAAGGCAGTTCCTTCAACTCCAGCTCAGTCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGTGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCTACTCTTTTAGTTCCTTCTAATCTTTGCTTTAAAATCACTTCAACCATCTTCTCAGGTCCTTGCAAACCTGGACTTGTGTTTAAGGTTAGTAATTCTATTTAAGTTTTGAATTTCTCAAGTAATACTTCAGTGCAAGTGCAGCCTGGACTACATTATATCCATCTCCACTCCACGCATCCCTTCAGTTAACTTTTTCATACGTGTTAACTTGGAATTAGACACTTAAGTGGAACGTAAGAGATGGGTGTAGCTCAAGATGTACTTAGGTACTTTGTAAGGAGCGAAAATACATTACAAGCTGAAGTTCCTCTCTGTTCGGAATGAGTGGGGTCATTCCACAACAATTTTTATATGGAACTTGGGACTTATGAGTGAAAATGAAACCAAACAGGTGGATGGGACTTTGACGCCACCGGATGGACCAGAGTCGTGGCCAAAAGCGGATAGTCCAAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACCGGAAGTGGAACCATTGAAGGGAATGGGCAGAAATGGTGGGAATTGCCATGCAAACCACATAGGGTACCTACATTTGCATTTATTTTCACTTCATCATTTCCTTTGTTATTGAATGTCTCTCCAATTTTCAATCCAAGGCTTAAACTTTCCTTTTTCAGGGTCCAAATGGGTCAACTCTCCCAGGACCATGTGACAGCCCTGCAGTAAGAAAAAACGCATCTTTGCTCTTTAATATTTCCCTCTTCATTCACTATTTATATAAATCAGAAATGCTGACTGAATTTTGGTTTTCTGGTTTTTTTCAGCTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTGAATAGTTTGAGAATCCAAAACAGCCCAATGTTCCATATGAAATTTGATGGGTGTGAGGGAGTTCTGATAGAAAGGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACCAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTATGAAACAATCGTTTAGTCTTGCTTGGTGATTTTCAAAGATACTACAGAGATTGATATAATGATTTACATATTTTGCTAACCACGTATTGTCCCAAATAGAGAGAAATATCATATTACAAATTTATGAAACATCAATAGAGTTAGAGAGTTATATGGTACATCCCTCTAAACCATAGGGAAAAATAAAAAAATAACATAAATATAAATACAATTTAGGTTTAAAACTGATCGACGCATCCCAGCACTAATTATCATTTCTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTAATTTGTGATTTGCAAATACAGGTGATGACTGCATTTCAATTGGACCTGGTTGTGCCAATGTGGCCATTGAAGGAGTGACTTGTGGGCCTAGTCACGGAATTAGGTACCAAAATGCCAATTCAATTTCTCTTGAATGTGAAAACTTCAATCTACAATTATTTGTTTATCACCACTCGAACAGAGAGTAACCCGTTGTTCTATGTTTGATTTTGGGAAAGAGCAGCATTGGGAGCTTAGGAGTCCACAATTCTCAAGCATGTGTTTCCAACATAACAGTAAGAAATGCAGTGATTAGAGATTCAGACAATGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTATCAAAGGATTGTCTAAACCAAACATCAGCTGTGTTTGTAAATCAGGTATTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGCGACTCAGTGGCTTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGGTTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTGA

mRNA sequence

ATGGAATTCAATGGGGTTCTCATTTTAATGATTTGGGTTATAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAGATGGAAGATACCATTTTCATAAGGGTAAAAAAGATGAAGATTCCCCTGTTTCTTCACCACCAGAGAAGGCAGTTCCTTCAACTCCAGCTCAGTCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGTGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCTACTCTTTTAGTTCCTTCTAATCTTTGCTTTAAAATCACTTCAACCATCTTCTCAGGTCCTTGCAAACCTGGACTTGTGTTTAAGGTGGATGGGACTTTGACGCCACCGGATGGACCAGAGTCGTGGCCAAAAGCGGATAGTCCAAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACCGGAAGTGGAACCATTGAAGGGAATGGGCAGAAATGGTGGGAATTGCCATGCAAACCACATAGGGGTCCAAATGGGTCAACTCTCCCAGGACCATGTGACAGCCCTGCACTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTGAATAGTTTGAGAATCCAAAACAGCCCAATGTTCCATATGAAATTTGATGGGTGTGAGGGAGTTCTGATAGAAAGGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACCAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGACCTGGTTGTGCCAATGTGGCCATTGAAGGAGTGACTTGTGGGCCTAGTCACGGAATTAGCATTGGGAGCTTAGGAGTCCACAATTCTCAAGCATGTGTTTCCAACATAACAGTAAGAAATGCAGTGATTAGAGATTCAGACAATGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTATCAAAGGATTGTCTAAACCAAACATCAGCTGTGTTTGTAAATCAGGTATTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGCGACTCAGTGGCTTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGGTTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTGA

Coding sequence (CDS)

ATGGAATTCAATGGGGTTCTCATTTTAATGATTTGGGTTATAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAGATGGAAGATACCATTTTCATAAGGGTAAAAAAGATGAAGATTCCCCTGTTTCTTCACCACCAGAGAAGGCAGTTCCTTCAACTCCAGCTCAGTCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGTGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCTGTTGAATCTGCTACTCTTTTAGTTCCTTCTAATCTTTGCTTTAAAATCACTTCAACCATCTTCTCAGGTCCTTGCAAACCTGGACTTGTGTTTAAGGTGGATGGGACTTTGACGCCACCGGATGGACCAGAGTCGTGGCCAAAAGCGGATAGTCCAAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACCGGAAGTGGAACCATTGAAGGGAATGGGCAGAAATGGTGGGAATTGCCATGCAAACCACATAGGGGTCCAAATGGGTCAACTCTCCCAGGACCATGTGACAGCCCTGCACTGATAAGATTCTTCATGAGCAGTAATTTGGCAGTGAATAGTTTGAGAATCCAAAACAGCCCAATGTTCCATATGAAATTTGATGGGTGTGAGGGAGTTCTGATAGAAAGGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACCAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGACCTGGTTGTGCCAATGTGGCCATTGAAGGAGTGACTTGTGGGCCTAGTCACGGAATTAGCATTGGGAGCTTAGGAGTCCACAATTCTCAAGCATGTGTTTCCAACATAACAGTAAGAAATGCAGTGATTAGAGATTCAGACAATGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATACTACTGCTTATCAAAGGATTGTCTAAACCAAACATCAGCTGTGTTTGTAAATCAGGTATTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGCGACTCAGTGGCTTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGGTTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTGA

Protein sequence

MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC
Homology
BLAST of Pay0005287 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 3.7e-199
Identity = 336/471 (71.34%), Postives = 382/471 (81.10%), Query Frame = 0

Query: 8   ILMIWVIAILFVENSSNV-DGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPSVPSDPY 67
           IL+I++I+ +   + +NV   RYH+HKG+     P+  PP    P   A     VPSDPY
Sbjct: 7   ILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPP--PLETANPPDQVPSDPY 66

Query: 68  PN-DPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKI 127
           P+ DP  G+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P    FKI
Sbjct: 67  PSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKI 126

Query: 128 TSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNG 187
           TSTIFSGPCKPGLVF++DG L PPDGPE WP+ D+  QWLVFYRLD  T +G GT+EGNG
Sbjct: 127 TSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNG 186

Query: 188 QKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGV 247
           QKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V  LRIQNSP FHMKFDGC+GV
Sbjct: 187 QKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGV 246

Query: 248 LIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPS 307
           LI  + ISSPKLSPNTDGIH+ NT+ VGIYNS++SNGDDCISIG GC++V I+GVTCGPS
Sbjct: 247 LINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPS 306

Query: 308 HGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVR 367
           HGISIGSLGVHNSQACVSNITVRN VIRDSDNGLR+KTWQGG+GSVS++LFENIQMENV 
Sbjct: 307 HGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVL 366

Query: 368 NCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSE 427
           NCIIVDQYYC SKDC N+TSAV V  V Y+NIKGTYDVR+ PIHFACSD+VACTNITMSE
Sbjct: 367 NCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSE 426

Query: 428 VELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEP-QNVAETSEYSC 476
           VELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 427 VELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Pay0005287 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 5.6e-86
Identity = 167/388 (43.04%), Postives = 229/388 (59.02%), Query Frame = 0

Query: 77  SDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESAT-LLVPSNLCFKITSTIFSGPCK 136
           S+ +  V +FGA G+G TDDT AF  AWK AC+ +  T +LVP N    +     SGPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 137 PGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKP 196
             L  ++ GT+  P+ P+ W +  + R+WL F+ L ++T+ G GT+ G GQ+WWE  CK 
Sbjct: 109 ARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK- 168

Query: 197 HRGPNGSTLPGPC-DSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIERLSISS 256
           H   N      PC  +P  + F    N+ V +L + +S   H+    C  V I  L + +
Sbjct: 169 HNHSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIA 228

Query: 257 PKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLG 316
           P  SPNTDGIHI  ++G+ I N+ +S GDDCISI      ++I  + CGP HGISIGSLG
Sbjct: 229 PATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLG 288

Query: 317 VHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYY 376
              S   V +ITV  A+I D+ NG+RIKTWQGGSG VS I+F NI+M NV N II+DQYY
Sbjct: 289 KSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYY 348

Query: 377 CLS-KDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEG 436
           C S K C NQTSA+ +  + + +++GT       I  +CSDS  C NI + +++L P  G
Sbjct: 349 CDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNG 408

Query: 437 ELVEDPFCWNAFGVQETLTIPPIDCLQE 462
           +   + FCW A+G       PP  CL +
Sbjct: 409 DGFTESFCWEAYGSSSGQVYPP-PCLSD 426

BLAST of Pay0005287 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.2e-85
Identity = 165/357 (46.22%), Postives = 218/357 (61.06%), Query Frame = 0

Query: 80  VFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLV 139
           +F+V  +GAVGDG  D T AF  AW+AAC   SA LLVP N  F + +  F+GPC+P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 140 FKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGP 199
           FKVDG +     P SW    + R WL F +L   TL G G I+G G++WW   CK     
Sbjct: 118 FKVDGIIAAYQNPASW---KNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK---WV 177

Query: 200 NGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIERLSISSPKLSP 259
           NG  +    D P  I+F  S+ L +  L++ NSP FH+ F  CEGV I  +SI++P+ SP
Sbjct: 178 NGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSP 237

Query: 260 NTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQ 319
           NTDGI I  +K   +  + I  GDDC++IG G +N+ IE + CGP HGISIGSLG  NS+
Sbjct: 238 NTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSR 297

Query: 320 ACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKD 379
           A VS + V  A   D+ NGLRIKTWQGGSG  S I++EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSAS 357

Query: 380 -CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGEL 436
            C NQ SAV +  V YKNI+GT       I   CSDS+ C +I +S++ L    G++
Sbjct: 358 ACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of Pay0005287 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 8.9e-84
Identity = 167/350 (47.71%), Postives = 211/350 (60.29%), Query Frame = 0

Query: 80  VFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLV 139
           VF+V  +GAVGDG  D T AF   W AAC   SA LLVP+N  F + + +F GPC+P L 
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117

Query: 140 FKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGP 199
           FKVDGT+     P  W  +   + WL F +L    L G+G I+G GQ+WW   CK     
Sbjct: 118 FKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV---V 177

Query: 200 NGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIERLSISSPKLSP 259
           NG T+    + P  I+   S ++ V  L + NSP FH+ F  CEGV I+ L I +P+ SP
Sbjct: 178 NGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP 237

Query: 260 NTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQ 319
           NTDGI I  +K   I   +I  GDDCI+IG G +N+ I+ + CGP HGISIGSLG  NS+
Sbjct: 238 NTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSR 297

Query: 320 ACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKD 379
           A VS++ V  A   D+ NGLRIKTWQGGSG  S I +EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSAS 357

Query: 380 -CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVEL 429
            C NQ SAV +  V YKNI GT       I   CSDSV CT I +S V L
Sbjct: 358 ACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSL 400

BLAST of Pay0005287 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.2e-81
Identity = 166/383 (43.34%), Postives = 226/383 (59.01%), Query Frame = 0

Query: 82  DVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGPCKPGLVFK 141
           +V DFGA GDG  DDT AF+ AWKAAC+  S+ +L+     + +    FSGPCK GL  +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQ 150

Query: 142 VDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNG 201
           + GT+   D    + K    R WLVF  +  + + G GTI GNG+ WW+  CK ++    
Sbjct: 151 IYGTIEASDDRSDYRK--DGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL-- 210

Query: 202 STLPGPC-DSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIERLSISSPKLSPN 261
                PC D+P  + F+ S ++ V +L+I+N+   H+ FD C  V    L +++P+ SPN
Sbjct: 211 -----PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPN 270

Query: 262 TDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQA 321
           TDGIH+  T+ + I + +I  GDDCISI  G   V +  +TCGP HGISIGSLG  NS+A
Sbjct: 271 TDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEA 330

Query: 322 CVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYC-LSKD 381
            VS++ V  A +  + NG+RIKTWQGGSGS S+I F+N++M NV N II+DQ YC   K 
Sbjct: 331 HVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKP 390

Query: 382 CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGELVEDP 441
           C  Q+SAV V  V+Y+NIKGT    N  I F CS    C  I + +V+ L  EG      
Sbjct: 391 CQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAKA 450

Query: 442 FCWNAFGVQETLTIPPIDCLQEG 463
            C N   + ET  + P  C +EG
Sbjct: 451 LC-NNVELSETGVVSP-HCQEEG 459

BLAST of Pay0005287 vs. ExPASy TrEMBL
Match: A0A5D3BVF7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00640 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.5e-283
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60
           MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Pay0005287 vs. ExPASy TrEMBL
Match: A0A1S3C7J5 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.5e-283
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60
           MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Pay0005287 vs. ExPASy TrEMBL
Match: A0A0A0KB54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 7.7e-264
Identity = 446/475 (93.89%), Postives = 452/475 (95.16%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60
           ME NGV I+MIWV+AILFVENSSNV+GRYHFHKGKKDEDS VSSPPE AVPSTPAQ +PS
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSTPAQPLPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISN                 GV
Sbjct: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISN-----------------GV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 458

BLAST of Pay0005287 vs. ExPASy TrEMBL
Match: A0A6J1EWI8 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 427/484 (88.22%), Postives = 448/484 (92.56%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDED--------SPV-SSPPEKAVP 60
           MEFN V  +M+W+ AIL  EN SNV GRYHFHKG+K ++        SPV S PPE +VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAQSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA   PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADPAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETS 476
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

BLAST of Pay0005287 vs. ExPASy TrEMBL
Match: A0A6J1KH63 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.1e-251
Identity = 424/484 (87.60%), Postives = 447/484 (92.36%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDED--------SPV-SSPPEKAVP 60
           MEFN V  +M+W+ AIL  EN SNV GRYHFHKG+K ++        SPV S PPE + P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPAQSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA   PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADQAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLI++LSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQ+LYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETS 476
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

BLAST of Pay0005287 vs. NCBI nr
Match: XP_008458305.1 (PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 984.6 bits (2544), Expect = 3.1e-283
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60
           MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Pay0005287 vs. NCBI nr
Match: XP_004138524.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein Csa_005406 [Cucumis sativus])

HSP 1 Score: 965.3 bits (2494), Expect = 1.9e-277
Identity = 463/475 (97.47%), Postives = 469/475 (98.74%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60
           ME NGV I+MIWV+AILFVENSSNV+GRYHFHKGKKDEDS VSSPPE AVPSTPAQ +PS
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSTPAQPLPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 475

BLAST of Pay0005287 vs. NCBI nr
Match: XP_038906648.1 (polygalacturonase At1g48100 [Benincasa hispida])

HSP 1 Score: 929.1 bits (2400), Expect = 1.5e-266
Identity = 448/480 (93.33%), Postives = 465/480 (96.88%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKD----EDSPV-SSPPEKAVPSTPA 60
           MEFNGV I+MIW++A L VENSSNV+GRYHFHKGKK+    EDSPV S PPEKAVPS+PA
Sbjct: 1   MEFNGVFIVMIWIVATLLVENSSNVNGRYHFHKGKKEEKPNEDSPVFSPPPEKAVPSSPA 60

Query: 61  QSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATL 120
           + VPSVPSDPYPN+P  GNS+SDCVFDVTDFGAVGDGC DDTAAFKAAWKAACAVESATL
Sbjct: 61  EPVPSVPSDPYPNEP--GNSSSDCVFDVTDFGAVGDGCADDTAAFKAAWKAACAVESATL 120

Query: 121 LVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTL 180
           LVPS LCFKITSTIFSGPCKPGLVFKVDGTL PP+GPESWPKADSPRQWLVFYRLDQMTL
Sbjct: 121 LVPSGLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPESWPKADSPRQWLVFYRLDQMTL 180

Query: 181 TGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 240
           TG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF
Sbjct: 181 TGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 240

Query: 241 HMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 300
           HMKFDGC+GVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV
Sbjct: 241 HMKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 300

Query: 301 AIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 360
           AI+GVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNG+RIKTWQGGSGSVSDIL
Sbjct: 301 AIDGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQGGSGSVSDIL 360

Query: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 420
           FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSD+
Sbjct: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDT 420

Query: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 476
           VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 478

BLAST of Pay0005287 vs. NCBI nr
Match: XP_022930280.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 882.1 bits (2278), Expect = 2.1e-252
Identity = 427/484 (88.22%), Postives = 448/484 (92.56%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDED--------SPV-SSPPEKAVP 60
           MEFN V  +M+W+ AIL  EN SNV GRYHFHKG+K ++        SPV S PPE +VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAQSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA   PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADPAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETS 476
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

BLAST of Pay0005287 vs. NCBI nr
Match: XP_023000015.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 878.6 bits (2269), Expect = 2.4e-251
Identity = 424/484 (87.60%), Postives = 447/484 (92.36%), Query Frame = 0

Query: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDED--------SPV-SSPPEKAVP 60
           MEFN V  +M+W+ AIL  EN SNV GRYHFHKG+K ++        SPV S PPE + P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPAQSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA   PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADQAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLI++LSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQ+LYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETS 476
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

BLAST of Pay0005287 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 695.7 bits (1794), Expect = 2.7e-200
Identity = 336/471 (71.34%), Postives = 382/471 (81.10%), Query Frame = 0

Query: 8   ILMIWVIAILFVENSSNV-DGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPSVPSDPY 67
           IL+I++I+ +   + +NV   RYH+HKG+     P+  PP    P   A     VPSDPY
Sbjct: 7   ILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPP--PLETANPPDQVPSDPY 66

Query: 68  PN-DPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSNLCFKI 127
           P+ DP  G+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P    FKI
Sbjct: 67  PSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKI 126

Query: 128 TSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNG 187
           TSTIFSGPCKPGLVF++DG L PPDGPE WP+ D+  QWLVFYRLD  T +G GT+EGNG
Sbjct: 127 TSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNG 186

Query: 188 QKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGV 247
           QKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V  LRIQNSP FHMKFDGC+GV
Sbjct: 187 QKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGV 246

Query: 248 LIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPS 307
           LI  + ISSPKLSPNTDGIH+ NT+ VGIYNS++SNGDDCISIG GC++V I+GVTCGPS
Sbjct: 247 LINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPS 306

Query: 308 HGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVR 367
           HGISIGSLGVHNSQACVSNITVRN VIRDSDNGLR+KTWQGG+GSVS++LFENIQMENV 
Sbjct: 307 HGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVL 366

Query: 368 NCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSE 427
           NCIIVDQYYC SKDC N+TSAV V  V Y+NIKGTYDVR+ PIHFACSD+VACTNITMSE
Sbjct: 367 NCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSE 426

Query: 428 VELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEP-QNVAETSEYSC 476
           VELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 427 VELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Pay0005287 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.4e-153
Identity = 271/484 (55.99%), Postives = 342/484 (70.66%), Query Frame = 0

Query: 4   NGVLILMIWVIAILFVENSSNVDGRYHFHKGK-------------KDEDSPVSSP--PEK 63
           N VL  M  ++ ++ +  + N     H HK K             K   S +S P  P  
Sbjct: 9   NQVLGFMTLILTLMSLSEARN-----HHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPP 68

Query: 64  AVPSTPAQSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAAC 123
             P +PA S+P  PSD    DP   N+    +++V  +GAVGDG TDDT AFK AW ++C
Sbjct: 69  GPPDSPAPSLPPSPSD----DPADDNNG---IYNVRKYGAVGDGETDDTEAFKTAWDSSC 128

Query: 124 AVESAT---LLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWL 183
             E+ T   LLVP    F I STIF+GPC+    F+VDGT+  PDGPESWP   S RQWL
Sbjct: 129 NNENNTDSVLLVPYGYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWL 188

Query: 184 VFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTL-PGPCDSPALIRFFMSSNLAV 243
           VFYR++ M L G G I+G GQKWW+LPCKPHR  N S +  GPCDSP  +RFFMSSNL V
Sbjct: 189 VFYRVNGMALKGEGVIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRV 248

Query: 244 NSLRIQNSPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDD 303
             L+I+NSP FH +FDGC+GV +E L I++P LSPNTDGIHIEN+  V IYNS+ISNGDD
Sbjct: 249 EGLQIKNSPQFHFRFDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDD 308

Query: 304 CISIGPGCANVAIEGVTCGP-SHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKT 363
           C+SIG G  +V I  +TCGP  HGISIGSLG HNS+ACVSNITVR++VI+ SDNG+RIKT
Sbjct: 309 CVSIGSGSYDVDIRNLTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKT 368

Query: 364 WQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDV 423
           WQGGSGSVS + F NI +++VRN II+DQYYC++KDC N+TSAVFV+ + Y+ IKGTYD+
Sbjct: 369 WQGGSGSVSGVTFNNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDI 428

Query: 424 RNTPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGE 468
           R+ P+HF CSD+V CTN+T+S++ELLP +GE+V DPFCWNA+G+ E L+IPP+ CL    
Sbjct: 429 RSPPMHFGCSDAVPCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDP 480

BLAST of Pay0005287 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 513.1 bits (1320), Expect = 2.4e-145
Identity = 246/414 (59.42%), Postives = 306/414 (73.91%), Query Frame = 0

Query: 55  AQSVPSVPSDPYPNDPGTGN-STSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAAC---AV 114
           AQS+   PS P PN     N + +  VFDVT FGA+GD  TDDT+AFK AW AAC     
Sbjct: 22  AQSIYLSPS-PAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGP 81

Query: 115 ESATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRL 174
           +SA LLVP   CF +  T F+GPC+  LV ++DG +  PDGP SWP ++  RQW++FYR+
Sbjct: 82  KSALLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWP-SNYQRQWMMFYRV 141

Query: 175 DQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQ 234
           + +++ GSG I G GQKWW LPCKPH+G NG+T  GPCDSP  IR F SS + +  +   
Sbjct: 142 NGLSIQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFM 201

Query: 235 NSPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGP 294
           NS  FH++FD C  V+++ + I +P  SPNTDGIHIENT  V I NSMISNGDDCISIG 
Sbjct: 202 NSAQFHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGA 261

Query: 295 GCANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGS 354
           GC NV I+ VTCGPSHGISIGSLGVHNSQA VSNITV N+ I +SDNG+RIKTWQGGSGS
Sbjct: 262 GCFNVDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGS 321

Query: 355 VSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHF 414
           VS I+F NI M NVRN I++DQYYC + +C NQTSAV ++ VLY NIKGTYD+R+ PIHF
Sbjct: 322 VSRIVFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHF 381

Query: 415 ACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEP 465
            CSDSV CTN+T++EV+L P +G+ +E+PFCWNA+G  + +T+PP+ CL +  P
Sbjct: 382 GCSDSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of Pay0005287 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 492.7 bits (1267), Expect = 3.4e-139
Identity = 234/415 (56.39%), Postives = 302/415 (72.77%), Query Frame = 0

Query: 57  SVPSVPSDPYPNDPGTGNSTS-----DCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 116
           S P  PS P PNDP    S S     + V+DV  +GAVG+G  DDT +FK AW +AC+  
Sbjct: 37  SQPPSPS-PNPNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACSNN 96

Query: 117 ----SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVF 176
               ++ L VP    F I STIF+GPC+    F+VDGT+ P DGP+SWP + + RQWL F
Sbjct: 97  KNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAF 156

Query: 177 YRLDQMTLTGSGTIEGNGQKWWELPCKPH-RGPNGSTLPGPCDSPALIRFFMSSNLAVNS 236
           YR++ M L G+G I+G GQ WW+LPCKPH +  N + L GPC+SPA +RFFMSSN+ V  
Sbjct: 157 YRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIVKG 216

Query: 237 LRIQNSPMFHMKFDGCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCI 296
           L I+NSP  H+K DGC  V I  L I SP  SPNTDGIHIEN+  V IYNS+ISNGDDC+
Sbjct: 217 LSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDDCV 276

Query: 297 SIGPGCANVAIEGVTCGP-SHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQ 356
           SIGPG  ++ I  +TCGP  HGISIGSLG  NS ACVSN+TVR++ I+ S+NG+RIKTWQ
Sbjct: 277 SIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQ 336

Query: 357 GGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRN 416
           GGSGSVS + F+NI ++ VRN II+DQYYC +K C N+TSAVFVN ++Y++IKGTYD+R+
Sbjct: 337 GGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRS 396

Query: 417 TPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQ 461
            P+HF CS++V CTN+T+S +ELLP + ++V  PFCWNA+G+ +  ++P I CL+
Sbjct: 397 PPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLK 450

BLAST of Pay0005287 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 370.2 bits (949), Expect = 2.6e-102
Identity = 198/457 (43.33%), Postives = 273/457 (59.74%), Query Frame = 0

Query: 35  KKDEDSPVSSPPEKAVPSTPAQSVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCT 94
           K ++ SPV S P +     P    P  P  P P  P   +      F+V DFGA GDG +
Sbjct: 83  KSNDGSPVVSQPPQVQQPPPPHVQP--PPTPLPLQPVEDSQQ----FNVLDFGAKGDGMS 142

Query: 95  DDTAAFKAAWKAACAVESATLLVPSNLCFKITSTIFSGP-CKPGLVFKVDGTLTPPDGPE 154
           DDT AF+AAW +AC VE++T+++P +  F +    FSGP C+  +VF+++G +  P   E
Sbjct: 143 DDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDTE 202

Query: 155 SWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWW------------------------ 214
           SW        W+ F +L  +T+ G+G I+G G  WW                        
Sbjct: 203 SW--GGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNSV 262

Query: 215 -ELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIE 274
            E P  P R      +P     P  +RF+ S ++ V  + IQNSP  H+KFD C  VL+ 
Sbjct: 263 QERPPMPIRSELNWRMPS--IKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVH 322

Query: 275 RLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGI 334
            +++SSP  SPNTDGIH++NT+ V I+ + ++ GDDCISI  GC+NV +  V CGP HGI
Sbjct: 323 DVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGI 382

Query: 335 SIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCI 394
           SIGSLG  +++ACVSNITVR+ V+ ++  G+RIKTWQGG GSV  ILF NIQ+  V+  I
Sbjct: 383 SIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPI 442

Query: 395 IVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVEL 454
           ++DQ+YC    C+N TSAV V  V Y+ I+GTY V+  P+HFACSDS  C ++ +S +EL
Sbjct: 443 VIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVK--PVHFACSDSFPCIDVQLSGIEL 502

Query: 455 LPHEGEL-VEDPFCWNAFGVQETLTIPPIDCLQEGEP 465
            P + +  + DPFCW  FG   + T+PPIDCLQ G+P
Sbjct: 503 KPVQLQYHMYDPFCWKTFGELNSATVPPIDCLQIGKP 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z13.7e-19971.34Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ55.6e-8643.04Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432121.2e-8546.22Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E78.9e-8447.71Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P353363.2e-8143.34Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BVF71.5e-283100.00Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G006... [more]
A0A1S3C7J51.5e-283100.00polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1[more]
A0A0A0KB547.7e-26493.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1[more]
A0A6J1EWI81.0e-25288.22polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 P... [more]
A0A6J1KH631.1e-25187.60polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=... [more]
Match NameE-valueIdentityDescription
XP_008458305.13.1e-283100.00PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturon... [more]
XP_004138524.11.9e-27797.47polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein C... [more]
XP_038906648.11.5e-26693.33polygalacturonase At1g48100 [Benincasa hispida][more]
XP_022930280.12.1e-25288.22polygalacturonase At1g48100-like [Cucurbita moschata][more]
XP_023000015.12.4e-25187.60polygalacturonase At1g48100-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G48100.12.7e-20071.34Pectin lyase-like superfamily protein [more]
AT1G02460.11.4e-15355.99Pectin lyase-like superfamily protein [more]
AT1G56710.12.4e-14559.42Pectin lyase-like superfamily protein [more]
AT4G01890.13.4e-13956.39Pectin lyase-like superfamily protein [more]
AT1G60590.12.6e-10243.33Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 269..290
e-value: 2800.0
score: 4.1
coord: 292..312
e-value: 5300.0
score: 2.1
coord: 322..343
e-value: 6.7
score: 15.7
coord: 242..268
e-value: 440.0
score: 9.6
coord: 351..372
e-value: 390.0
score: 9.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 112..428
e-value: 3.3E-85
score: 286.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 299..312
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 71..473
e-value: 4.9E-119
score: 400.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..74
NoneNo IPR availablePANTHERPTHR31375:SF142POLYGALACTURONASE PLANT-LIKE PROTEINcoord: 6..466
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 6..466
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 79..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0005287.1Pay0005287.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity