Pay0005240 (gene) Melon (Payzawat) v1

Overview
NamePay0005240
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptionmitotic checkpoint serine/threonine-protein kinase BUB1
Locationchr01: 2297365 .. 2301623 (-)
RNA-Seq ExpressionPay0005240
SyntenyPay0005240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGAATTGCAAAAAAGGCGCTCTTCTTGGAGCTTGGAGCCAATGTACAGTCCGAGTTGGCATCTTCCATTTCCCTTCGTCCATCGACGACCAGAAAGCTACCAGCAATGACGATTTTCTTCGCCGATTCTCAAATCTCCTCCACCACAGCATCCGCTCAAGACCCTTTGTTGCCTTGGCTCTGGTATTCCTTCATTTCTGTTGTGTGATAGAGCTTATTTACAGCCATTCTTACATTCAACCTCAGGAAAATTGGTCGTGCTATTTGGTTCCATTTGTAGGTCTATCAAGGGTGCTCTTGAAAATTCAACTTCAGGCAATGTTTCTGGGTCAGAACTCGCCAAGCTTCTCTCCGATTGCATTGGAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTGTGCGTAATTATCTTTCTTTAACTGTTATATTTTGTGGGTTTAACTTATCGTCTTTGCTTCATACGTGTGGTTGATTAGATAACTTGTATGTGAGCATGTGCTATGTTTTGGATGAAGTATCTTTTGAAATTTGCTTATGCTATCTATTTCCCTACACTTCTCTTTCCTTTTCCTATGATGCTCACTTTAGATTATTTGTATAATTTTGTTTTATGTGTAAATGCTTGGATCTCTTTGAAAAGTTAGGCATTTCCGACAATTTTTAATCCGGTTGTTGATTTTGAATAAAGATTAGCATTATGTTATTCGTGCAAATAGATTGGTATGAGGGAGGATTTTGAAACTGGGTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTTCTCGAATCCAAGGGGAGGTTGAACGACGCTAACATAGTTTATGAGCTTGGGTTGATGAGGTATTCCTATCTCTCTCTCTCATTGACTGCTGTTAAATACGTGTAGATTATGCCTGTGAAAATTTTTGTTGATTAGGATTTTTTTGTTTGGTTGTGTTCTGTGTTGTTTAGTTCCTTACATTTTAAAGTGATAAACTGATAATAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAAGATTGTTTATTGATAGGATATCTGAGTTGGTCAATACTCATTCAATGCAAGTGGTACGTGATCTTAAAGCATAAAACCATTGAAGTTCACATTCCTTCAATCGTATAATTTAAGAAATTTGACACGGTTCGTTTTCATTTTTGTAGAATGATGTCAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAACAAGATTAATCCAAAGATTATGAAGTATGATGTAAGAATTCGCAGTTTTCTTTCGCCAAATGATGAAAGCAAGCTGGTTATATTGTTCAAATAAATTACTGTCAAGCTAACCTTAATATGAATTTAATGTGTGTTTCAGGGTTACCATTCAAGTACCAAAGCTTACTCTAAGAAGATGAATCTAAAGTCATTGCGCAATTCATCAAGAAATAAGATTATTGAGATTGGTAATTTTTTATGTTACATTATAGTTAGATTTGTCACCTAAACGGATAGAACTACCTTCAGTTTATCCTTCAAACGAACAAGCTTTCTTGAAATATTGTATAAGAAATGGAGGAAATAGTTTTCTTATGCCACGGATTCATTTTTCATTATAATGATATCCTTACCAACATTTACAGGTGGAAAGAAGTATGAGATTAAAGGTTGTGCCGGTACAGGTGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCGGATGATGTAGTGGCACTGAAGGTAAAATCTCTTATTGATTGATATGCAATATGCATATGTTCTTTATTTCCAGCATTCCTTATATGTCATACCTGAATGTGAACTAGTTGGATATCATGATTCCCTTCAAATGCTTCCCAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTTGATCTACGTGTCGCTGCTAAGGAAGTATGTACTAGGCTACTAGCCCCTTATTTTCATCAAGCATTTATTACCTCTAAATGTCAGAAACTCGATTTATACTCTTACTCTATCTCTCATAATGTCAGAGGTCAAGTTTTGGTTGTGCTCATGCCATGTATCTCTATTCTGACTACAGCATACTTATATGTGATTACTTGGCTAATGGAACATTGCAGGTTAGTATACAGATCACTGAACCTTCTATACTGTATGACATTTTAAAATATTCACTGTAAGTAACTATCTTGTCAAAACCTCGTGCAGGATGCCATAAATTCGTATGTAGTTCTTGGAAAGACAATGGAAGAAGTTTTATGCATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTGCACAGCATTGGCATTATCCATGGGGATTTTAAACCGGACAATCTTCTTGTTCGTTATGCGGGGTAAATATATTCTGGTTCTAGATCTTTATTGATGACATCCATCTTTCTGAGAGTTGATTTCCTTATATAATTGTTATATACTCGGGAAAAAAAGAATACTCGGGAAAAAAAGAAAAGAACCATAGTAAATTAAAAGATGAGACCTCTAGTTGTTATATACAACTTCAGCCTACCAACAGTGATTAGCATCCGACTGGAGAGTGTACTCCAATTCAATAGTTCTTTACCAATTTATTTTATTTAACCGTTATGCAGTGAAAACCTAACAGAAGATGATTTTCAGGATCGTCGTGGTCCTTGGCAAGATCAGGTATGTAGTATCATCCCCTTTTTTGTTCCCAAGTCATTGCATGTCTACTTGGAATGGCCTTTTAAGTGCTGAAAAAAGTGTTTTTAAATGAAAAAAATTGCGTTTTAAGCACTTTTAAAGGCATTCCAAGCCGACCCTATTAACCGTGAGCAAACAAGATTATTTGTAGTTTTTATATTATAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCAACTCTTTCCCGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCCGGCTTTCGTTGTCCAGAAATGCTAGAGAATAGACCTTGGAAATATCAGGTCAGTTTCTTCTGCAATGTGGTTGTTTATAACAGCTCTAGAGAAAACCCACTGTAAATAAGAAAACAGAAGCACTACCAACAGCATCTTGTCGTTTGTACAGGTAGACATCTATGGCTTATGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAGGCATCATCCGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTAGTTACAATTTCTGACTTTTTAGTTTCTATTTAGTATTCCTCTTGTTTTTTGTTTTAAAAACTAAAGCTTATTTTCTCTCGATTTCTTACCATACTTTTCATCTTTCTTAAAAACAAAAGTTTGATTTAGTCAAATTCCAAAAACAAAAACAAAATTTTAAGAACTACTTTCTTTAGTTTCCAAATTATGGCTTGGTTTTGAAAACATAGCTAAAAAAGTAGAAAACAAATCAATAAATTTAGATGTCGAAGAAGAGTTGTTAAACCCGATTTTGAAAAATAAACCCGATTTTGAAAAATAAAATACAAAATATCAAATAGTTAACGGAGAGTGGTAATTGTCTGTTTTCTGCTTTTTCCTGATAAATATGTTGTGCATGAATTTTAACTCTTCTAGATTCTTTGTAGAGTTTTAGATGGACAACTTTGTTTTATAGAGAGCAACTGCCCTCTTCACCTCCTCCCCCTGACTTGTTCTCTCTGTTCCTCTATAGGTATTGGAACATTGAATTATGGAAGAATCTGTTCACGAAGTTGCTTAATATCACACCAGGTCAATACAACAAAGAGTTGTTGCAGAGTTTAAGGAAATCTTTCCAGGATTACATGTGCTCCAATCCTCAACTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCTTCCTTGTGTACTTCTTAATCTAGAACCTCCTCTTGAATATATGTAAATTCCCAAAAAAAATGTAGGTGGTTCATGTCTGCCTCTAGCAAACTTAGATTAGCTCATTGCTCTCATGATGAAATGTTTGTATTCTTTTTTCTTGTCTGTATTATTAAAAACAAAAAAAGTTAGAAGAGTTGTAACTAAGTGGTTTCTTTGAAATACTGTGTCTTGTGGGGTTCATTGTTTATCCTAAAGGTAAGCTGTAATGTAGTAGGTTGCAGATATTCATTTAGATTATAT

mRNA sequence

ATTTGAATTGCAAAAAAGGCGCTCTTCTTGGAGCTTGGAGCCAATGTACAGTCCGAGTTGGCATCTTCCATTTCCCTTCGTCCATCGACGACCAGAAAGCTACCAGCAATGACGATTTTCTTCGCCGATTCTCAAATCTCCTCCACCACAGCATCCGCTCAAGACCCTTTGTTGCCTTGGCTCTGGTCTATCAAGGGTGCTCTTGAAAATTCAACTTCAGGCAATGTTTCTGGGTCAGAACTCGCCAAGCTTCTCTCCGATTGCATTGGAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGGTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTTCTCGAATCCAAGGGGAGGTTGAACGACGCTAACATAGTTTATGAGCTTGGGTTGATGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAAGATTGTTTATTGATAGGATATCTGAGTTGGTCAATACTCATTCAATGCAAGTGAATGATGTCAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAACAAGATTAATCCAAAGATTATGAAGTATGATGGTTACCATTCAAGTACCAAAGCTTACTCTAAGAAGATGAATCTAAAGTCATTGCGCAATTCATCAAGAAATAAGATTATTGAGATTGGTGGAAAGAAGTATGAGATTAAAGGTTGTGCCGGTACAGGTGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCGGATGATGTAGTGGCACTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTTGATCTACGTGTCGCTGCTAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGCCATGTATCTCTATTCTGACTACAGCATACTTATATGTGATTACTTGGCTAATGGAACATTGCAGGATGCCATAAATTCGTATGTAGTTCTTGGAAAGACAATGGAAGAAGTTTTATGCATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTGCACAGCATTGGCATTATCCATGGGGATTTTAAACCGGACAATCTTCTTGTTCGTTATGCGGGTGAAAACCTAACAGAAGATGATTTTCAGGATCGTCGTGGTCCTTGGCAAGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCAACTCTTTCCCGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCCGGCTTTCGTTGTCCAGAAATGCTAGAGAATAGACCTTGGAAATATCAGGTAGACATCTATGGCTTATGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAGGCATCATCCGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAATTATGGAAGAATCTGTTCACGAAGTTGCTTAATATCACACCAGGTCAATACAACAAAGAGTTGTTGCAGAGTTTAAGGAAATCTTTCCAGGATTACATGTGCTCCAATCCTCAACTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCTTCCTTGTGTACTTCTTAATCTAGAACCTCCTCTTGAATATATGTAAATTCCCAAAAAAAATGTAGGTGGTTCATGTCTGCCTCTAGCAAACTTAGATTAGCTCATTGCTCTCATGATGAAATGTTTGTATTCTTTTTTCTTGTCTGTATTATTAAAAACAAAAAAAGTTAGAAGAGTTGTAACTAAGTGGTTTCTTTGAAATACTGTGTCTTGTGGGGTTCATTGTTTATCCTAAAGGTAAGCTGTAATGTAGTAGGTTGCAGATATTCATTTAGATTATAT

Coding sequence (CDS)

ATGACGATTTTCTTCGCCGATTCTCAAATCTCCTCCACCACAGCATCCGCTCAAGACCCTTTGTTGCCTTGGCTCTGGTCTATCAAGGGTGCTCTTGAAAATTCAACTTCAGGCAATGTTTCTGGGTCAGAACTCGCCAAGCTTCTCTCCGATTGCATTGGAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGGTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTTCTCGAATCCAAGGGGAGGTTGAACGACGCTAACATAGTTTATGAGCTTGGGTTGATGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAAGATTGTTTATTGATAGGATATCTGAGTTGGTCAATACTCATTCAATGCAAGTGAATGATGTCAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAACAAGATTAATCCAAAGATTATGAAGTATGATGGTTACCATTCAAGTACCAAAGCTTACTCTAAGAAGATGAATCTAAAGTCATTGCGCAATTCATCAAGAAATAAGATTATTGAGATTGGTGGAAAGAAGTATGAGATTAAAGGTTGTGCCGGTACAGGTGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCGGATGATGTAGTGGCACTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTTGATCTACGTGTCGCTGCTAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGCCATGTATCTCTATTCTGACTACAGCATACTTATATGTGATTACTTGGCTAATGGAACATTGCAGGATGCCATAAATTCGTATGTAGTTCTTGGAAAGACAATGGAAGAAGTTTTATGCATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTGCACAGCATTGGCATTATCCATGGGGATTTTAAACCGGACAATCTTCTTGTTCGTTATGCGGGTGAAAACCTAACAGAAGATGATTTTCAGGATCGTCGTGGTCCTTGGCAAGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCAACTCTTTCCCGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCCGGCTTTCGTTGTCCAGAAATGCTAGAGAATAGACCTTGGAAATATCAGGTAGACATCTATGGCTTATGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAGGCATCATCCGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAATTATGGAAGAATCTGTTCACGAAGTTGCTTAATATCACACCAGGTCAATACAACAAAGAGTTGTTGCAGAGTTTAAGGAAATCTTTCCAGGATTACATGTGCTCCAATCCTCAACTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCTTCCTTGTGTACTTCTTAA

Protein sequence

MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Homology
BLAST of Pay0005240 vs. ExPASy Swiss-Prot
Match: F4IVI0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana OX=3702 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 2.8e-195
Identity = 316/518 (61.00%), Postives = 403/518 (77.80%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK ++E+  +G  SG +L KLL DCI  +  + +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY W A FLE KG    AN VY+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKRLFIDRISELVNTHSMQ-VNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKY 195
           LK+A  LF+ RIS+     S++ V D +++T     ++NPW +S +N L++KI P+++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 DGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           DGYH S K +  K NL SL+N SRNKIIEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQD 315
           VVALK+Q PPFPWEF+MYRQLD R+   +RSSFG A  +++YSDYSIL+CDYL++GTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDF 375
            INSYVV+GK+MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLL+RY  ENLTE  F
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 QDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYG 435
            ++ G W  +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E++PWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 LCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGQYNK 495
           LCV+VH MLH  YME+EKK S DG ++ +P++SFKRYW ++LWK LFTKLLN    + + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           E L++LRKS ++Y+CS+P+L++KL ELL KQR SLC+S
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

BLAST of Pay0005240 vs. ExPASy Swiss-Prot
Match: P41695 (Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BUB1 PE=1 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 6.1e-49
Identity = 112/343 (32.65%), Postives = 187/343 (54.52%), Query Frame = 0

Query: 173 NPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSL----RNSSRNKIIEI--GG 232
           NP S+++    L++I+P + +Y+ +++  +       LK +    RN ++N I++    G
Sbjct: 643 NPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKKIHRVSRNENKNPIVDFKKTG 702

Query: 233 KKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKE-RS 292
             Y I+G  G GG+A VY +  +S+   + ALK++ P   WE+Y+  Q++ R+       
Sbjct: 703 DLYCIRGELGEGGYATVYLA--ESSQGHLRALKVEKPASVWEYYIMSQVEFRLRKSTILK 762

Query: 293 SFGCAHAMYLYSDYSILICDYLANGTLQDAIN----SYVVLGKTMEEVLCIYYTIEMLYM 352
           S   A A++L+ D S L+ +Y + GT+ D IN      +     M+E LC++ T+E++ +
Sbjct: 763 SIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITVELMKV 822

Query: 353 LETLHSIGIIHGDFKPDNLLVRY--AGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQL 412
           LE +H +GIIHGD KPDN ++R    GE L     ++    W+++G+ L+D+GR  D+ L
Sbjct: 823 LEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRSFDMTL 882

Query: 413 FPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGD 472
            P   +FK + +     C EM   +PW Y+ D YGL  V+H+ML G ++E  +  +    
Sbjct: 883 LPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQLQNGRCK 942

Query: 473 FVYLPKSSFKRYWNIELWKNLFTKLLNITPGQYNKELLQSLRK 503
                K+ FKRYW  E+W  +F  LLN   GQ + + L    K
Sbjct: 943 L----KNPFKRYWKKEIWGVIFDLLLN--SGQASNQALPMTEK 977

BLAST of Pay0005240 vs. ExPASy Swiss-Prot
Match: O08901 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090 GN=Bub1 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.0e-47
Identity = 114/354 (32.20%), Postives = 188/354 (53.11%), Query Frame = 0

Query: 173  NPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIK 232
            NPW   ++  LL+ ++  +  Y    S+T  +  K  L +++  +     ++G     + 
Sbjct: 714  NPWDDELILKLLSGLSKPVTSY----SNTFEWQSK--LPAIKTKTE---YQLGSLLVYVN 773

Query: 233  GCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFG 292
               G G FAQV+++      ++  +    LK+Q P   WEFY+  QL  R+  +    F 
Sbjct: 774  HLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMFI 833

Query: 293  CAHAMYLYSDYSILICDYLANGTLQDAINSYV-VLGKTMEEVLCIYYTIEMLYMLETLHS 352
              ++ +L+ + SIL+ +  + GTL + IN Y     K M + L + + I MLYM+E +HS
Sbjct: 834  KFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHS 893

Query: 353  IGIIHGDFKPDNLLVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFK 412
              IIHGD KPDN ++ +      ++D           GL L+D G+ ID++LFP+   F 
Sbjct: 894  CEIIHGDIKPDNFILGHRFLEQADEDLA--------TGLALIDLGQSIDMKLFPKGTVFT 953

Query: 413  GDCRTSGFRCPEMLENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 472
            G C TSGF+CPEML N+PW YQ+D +G+   ++ ML GSYM+V+ +       V+ P+  
Sbjct: 954  GKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKVKNEGG-----VWKPEGL 1013

Query: 473  FKRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKEL 522
            F+R  ++++W+  F  +LNI P  +N   L  LR++ +  +    Q   K+K L
Sbjct: 1014 FRRLPHLDMWEEFFHIMLNI-PDCHNLPSLDFLRQNMKKLL--EQQYSNKIKTL 1042


HSP 2 Score: 57.4 bits (137), Expect = 5.8e-07
Identity = 40/133 (30.08%), Postives = 58/133 (43.61%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           +  DPL  W   IK   EN          L  LL   +  F     Y ND RF+      
Sbjct: 19  TGNDPLGEWESFIKWVEENFPDNK---EYLMTLLEHLMKEFLHKKNYHNDSRFINYCLKF 78

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
                D    F+ L  Q I   +S +Y+  A  LE++G L  A+ +++ G+   AEP E 
Sbjct: 79  AEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKEL 138

Query: 136 LKKAKRLFIDRIS 149
           L++  RLF  R++
Sbjct: 139 LQQQYRLFQARLT 148

BLAST of Pay0005240 vs. ExPASy Swiss-Prot
Match: O43683 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.7e-46
Identity = 115/359 (32.03%), Postives = 183/359 (50.97%), Query Frame = 0

Query: 173  NPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIK 232
            NPW   ++  LL+ ++  +  Y         +  +  L +++  +     ++G K   + 
Sbjct: 740  NPWDDKLIFKLLSGLSKPVSSY------PNTFEWQCKLPAIKPKTE---FQLGSKLVYVH 799

Query: 233  GCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFG 292
               G G FAQVY++      D+       LK+Q P  PWEFY+  QL  R+    +  F 
Sbjct: 800  HLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFM 859

Query: 293  CAHAMYLYSDYSILICDYLANGTLQDAINSYV-VLGKTMEEVLCIYYTIEMLYMLETLHS 352
              ++ +L+ + S+L+ +  + GTL +AIN Y     K M + L I + + MLYM+E +H 
Sbjct: 860  KFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHD 919

Query: 353  IGIIHGDFKPDNLLVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFK 412
              IIHGD KPDN ++   G    E D +D        GL L+D G+ ID++LFP+   F 
Sbjct: 920  CEIIHGDIKPDNFIL---GNGFLEQDDEDD----LSAGLALIDLGQSIDMKLFPKGTIFT 979

Query: 413  GDCRTSGFRCPEMLENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 472
              C TSGF+C EML N+PW YQ+D +G+   V+ ML G+YM+V+ +          P+  
Sbjct: 980  AKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGE-----CKPEGL 1039

Query: 473  FKRYWNIELWKNLFTKLLNITPGQYNKE---LLQSLRKSFQDYMCSNPQLIRKLKELLV 524
            F+R  ++++W   F  +LNI    +      L Q L+K FQ +  +    IR L+  L+
Sbjct: 1040 FRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNK---IRALRNRLI 1074

BLAST of Pay0005240 vs. ExPASy Swiss-Prot
Match: O94751 (Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bub1 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.0e-44
Identity = 119/378 (31.48%), Postives = 185/378 (48.94%), Query Frame = 0

Query: 155 SMQVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSL- 214
           +M   +   S     + INP   S+ + L   + P +++   YH    +++   +++S  
Sbjct: 623 TMPYEEAMASLSNLPTLINPLDQSLRDLLFQVLRPSLLRDKDYHEHETSFALVEHIESFV 682

Query: 215 -------------RNSSRNK-------IIEIGGKKYEIKGCAGTGGFAQVY--KSYLDSN 274
                        R+S+R+        +         +    G G FA VY  KS +++ 
Sbjct: 683 SKIKPKAGGPGRRRSSNRHSLDGPEFHLFYPPNTNLSVISKLGQGAFAPVYLVKSKIETE 742

Query: 275 PDDV------------VALKIQTPPFPWEFYMYRQLDLRV-AAKERSSFGCAHAMYLYSD 334
             DV             ALKI+TPP  +EFY+ RQ   R+   +E +S    H ++++ D
Sbjct: 743 NGDVSQGGAENNESKLFALKIETPPSCFEFYLTRQAMTRLKGLRETNSILPVHQLHMFHD 802

Query: 335 YSILICDYLANGTLQDAINSY---VVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDF 394
            S L+ DY   G++ D +NS          M+E+L ++++IE L ++E LH+  IIHGD 
Sbjct: 803 TSHLLMDYRPQGSILDLVNSMHNSTFSSSGMDEILVVFFSIEFLRIIEALHTHKIIHGDL 862

Query: 395 KPDNLLVRYAGENLTEDDFQDRRGP-----WQDQGLCLVDWGRGIDLQLFPENVEFKGDC 454
           K DN L+R   E + + ++     P     W  +G+ L+D+GRGIDL LF E V+F  D 
Sbjct: 863 KADNALLRL--ETVADSEWSPIYSPEGLYGWSFKGIYLIDFGRGIDLSLFEEKVKFIADW 922

Query: 455 RTSGFRCPEMLENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKR 489
            T    C EM E RPW YQ+D +GL  +++TML G Y+E   +   +G    +     KR
Sbjct: 923 DTDLQDCIEMREGRPWTYQIDYHGLAAIIYTMLFGQYIETRIEV-INGQRRQVLTQRMKR 982

BLAST of Pay0005240 vs. ExPASy TrEMBL
Match: A0A5D3C9K3 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003120 PE=3 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 529/532 (99.44%), Postives = 530/532 (99.62%), Query Frame = 0

Query: 1    MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 816  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 875

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 876  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 935

Query: 121  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 936  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 995

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 996  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1055

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 1056 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1115

Query: 301  ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1116 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1175

Query: 361  LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            LVRYAGENLTEDDFQ+RRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1176 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1235

Query: 421  LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1236 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1295

Query: 481  FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 1296 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 1347

BLAST of Pay0005240 vs. ExPASy TrEMBL
Match: A0A1S3C1A0 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 GN=LOC103495479 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 529/532 (99.44%), Postives = 530/532 (99.62%), Query Frame = 0

Query: 1   MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           MTI FADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 1   MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           LVRYAGENLTEDDFQ+RRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Pay0005240 vs. ExPASy TrEMBL
Match: A0A5A7SMW7 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003080 PE=3 SV=1)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 528/532 (99.25%), Postives = 530/532 (99.62%), Query Frame = 0

Query: 1    MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 843  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 902

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 903  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 962

Query: 121  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 963  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 1022

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 1023 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1082

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 1083 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1142

Query: 301  ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1143 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1202

Query: 361  LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            LVRYAGENLTEDDFQ+RRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1203 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1262

Query: 421  LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1263 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1322

Query: 481  FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKL+ELLVKQRASLCTS
Sbjct: 1323 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLQELLVKQRASLCTS 1374

BLAST of Pay0005240 vs. ExPASy TrEMBL
Match: A0A0A0K5T0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1)

HSP 1 Score: 1060.1 bits (2740), Expect = 3.1e-306
Identity = 514/532 (96.62%), Postives = 522/532 (98.12%), Query Frame = 0

Query: 1   MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M IFFADSQIS+TTAS QDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI NSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           +DLLNKI+PKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAGE++TED FQ+R GPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Pay0005240 vs. ExPASy TrEMBL
Match: A0A6J1EXJ7 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX=3662 GN=LOC111437097 PE=4 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.7e-276
Identity = 458/532 (86.09%), Postives = 487/532 (91.54%), Query Frame = 0

Query: 1   MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M I   DSQI++TT SAQDPLLPWLWSIK ALENSTSGNVSG  LA L+SDCI  F G V
Sbjct: 58  MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 117

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIW A FLESKGRLNDAN+
Sbjct: 118 KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 177

Query: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
           +YELGL+RNAEPLEWLKKAK LFIDR+SELVNTHSM++ND  ESTKF ESY+NPWSSS+M
Sbjct: 178 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 237

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            DLL+KIN KI+KY+GYHSS K Y KKM L SLRNSSRNK+IEIGGKKYEIKGCAGTGGF
Sbjct: 238 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 297

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFY+YRQLD RV  KERSSFGCAH MYLYSDYS
Sbjct: 298 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 357

Query: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLA+GTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLH+IGIIHGDFKPDNL
Sbjct: 358 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 417

Query: 361 LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           L+RYA   LTED F++R GPW DQGLCLVDWGRGIDLQLFPENVEF+GDCRTSGFRC EM
Sbjct: 418 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 477

Query: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYG CVVVHTMLHGSYM+VEKK SSDG FVYLPK+SFKRYWN+ELWKNL
Sbjct: 478 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 537

Query: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNI  G YNKELLQS+R+SFQDYMCSNPQLIRKLKELL KQRASLCT+
Sbjct: 538 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 589

BLAST of Pay0005240 vs. NCBI nr
Match: TYK06996.1 (protein TPX2 [Cucumis melo var. makuwa])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 529/532 (99.44%), Postives = 530/532 (99.62%), Query Frame = 0

Query: 1    MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 816  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 875

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 876  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 935

Query: 121  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 936  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 995

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 996  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1055

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 1056 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1115

Query: 301  ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1116 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1175

Query: 361  LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            LVRYAGENLTEDDFQ+RRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1176 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1235

Query: 421  LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1236 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1295

Query: 481  FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 1296 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 1347

BLAST of Pay0005240 vs. NCBI nr
Match: XP_008455277.1 (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 529/532 (99.44%), Postives = 530/532 (99.62%), Query Frame = 0

Query: 1   MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           MTI FADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 1   MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           LVRYAGENLTEDDFQ+RRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Pay0005240 vs. NCBI nr
Match: KAA0031543.1 (protein TPX2 [Cucumis melo var. makuwa])

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 528/532 (99.25%), Postives = 530/532 (99.62%), Query Frame = 0

Query: 1    MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 843  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 902

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 903  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 962

Query: 121  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 963  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 1022

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 1023 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1082

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 1083 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1142

Query: 301  ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1143 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1202

Query: 361  LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            LVRYAGENLTEDDFQ+RRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1203 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1262

Query: 421  LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1263 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1322

Query: 481  FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKL+ELLVKQRASLCTS
Sbjct: 1323 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLQELLVKQRASLCTS 1374

BLAST of Pay0005240 vs. NCBI nr
Match: XP_004136844.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus] >KGN43647.1 hypothetical protein Csa_017323 [Cucumis sativus])

HSP 1 Score: 1060.1 bits (2740), Expect = 6.4e-306
Identity = 514/532 (96.62%), Postives = 522/532 (98.12%), Query Frame = 0

Query: 1   MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M IFFADSQIS+TTAS QDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI NSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           +DLLNKI+PKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAGE++TED FQ+R GPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

BLAST of Pay0005240 vs. NCBI nr
Match: XP_038888854.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hispida] >XP_038888855.1 mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.2e-297
Identity = 495/532 (93.05%), Postives = 514/532 (96.62%), Query Frame = 0

Query: 1   MTIFFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M IF+ DSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSG ELAKLLSDCI NFSG+V
Sbjct: 1   MAIFYPDSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELAKLLSDCIENFSGDV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIG+SLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGHSLLYIWLAVFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSS M
Sbjct: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSTM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240
           NDLL+KI+PKIMKYDGYHSSTKAYSKKM LKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLSKISPKIMKYDGYHSSTKAYSKKMTLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300
           AQVY+SYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV  KERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYRSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVNGKERSSFGCAHAMYLYSDYS 300

Query: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           ILICD+LA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDFLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAG+NLTED+FQ+R GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGDNLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWK+QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPK+SFKRYWNIELWKNL
Sbjct: 421 LENKPWKFQVDIYGFCVVVHTMLHGSYMEVEKKPSSDGDFVYLPKTSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPG YNKEL+QS+RKSFQDYMCSNPQLIRKLKELL KQRASLC +
Sbjct: 481 FTKLLNITPGDYNKELIQSIRKSFQDYMCSNPQLIRKLKELLAKQRASLCNA 532

BLAST of Pay0005240 vs. TAIR 10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases )

HSP 1 Score: 682.9 bits (1761), Expect = 2.0e-196
Identity = 316/518 (61.00%), Postives = 403/518 (77.80%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK ++E+  +G  SG +L KLL DCI  +  + +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY W A FLE KG    AN VY+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKRLFIDRISELVNTHSMQ-VNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKY 195
           LK+A  LF+ RIS+     S++ V D +++T     ++NPW +S +N L++KI P+++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 DGYHSSTKAYSKKMNLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           DGYH S K +  K NL SL+N SRNKIIEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQD 315
           VVALK+Q PPFPWEF+MYRQLD R+   +RSSFG A  +++YSDYSIL+CDYL++GTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDF 375
            INSYVV+GK+MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLL+RY  ENLTE  F
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 QDRRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYG 435
            ++ G W  +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E++PWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 LCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGQYNK 495
           LCV+VH MLH  YME+EKK S DG ++ +P++SFKRYW ++LWK LFTKLLN    + + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           E L++LRKS ++Y+CS+P+L++KL ELL KQR SLC+S
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

BLAST of Pay0005240 vs. TAIR 10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 74.3 bits (181), Expect = 3.2e-13
Identity = 55/190 (28.95%), Postives = 88/190 (46.32%), Query Frame = 0

Query: 19  DPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G    S L  +   C+  F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGG-ECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI  A  +E K ++  AN ++ LG+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKRLFIDRISELVNTHSMQ---VNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYD 198
           A + F+ R     NT   Q    N+   S  F  + ++   ++     L   NP+  K  
Sbjct: 200 AYKKFMVRTMRRSNTADEQEPKENNDLPSRSFG-TLLSRGDNNARRQALGSSNPQAKKLK 259

Query: 199 GYHSSTKAYS 206
              SS   ++
Sbjct: 260 PNQSSKTPFA 267

BLAST of Pay0005240 vs. TAIR 10
Match: AT2G33560.1 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 73.9 bits (180), Expect = 4.2e-13
Identity = 51/188 (27.13%), Postives = 89/188 (47.34%), Query Frame = 0

Query: 19  DPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G    S L  +   C+  F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGG-ECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI  A  +E K ++  AN ++ LG+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKRLFIDRISELVNTHSMQVNDVSE-STKFAESYINPWSSSIMNDLLNKINPKIMKYDGY 198
           A + F+ R     NT   +  + ++  ++   + ++   ++     L   NP+  K    
Sbjct: 200 AYKKFMVRTMRRSNTADEEPKENNDLPSRSFGTLLSRGDNNARRQALGSSNPQAKKLKPN 259

Query: 199 HSSTKAYS 206
            SS   ++
Sbjct: 260 QSSKTPFA 266

BLAST of Pay0005240 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 73.2 bits (178), Expect = 7.2e-13
Identity = 38/132 (28.79%), Postives = 66/132 (50.00%), Query Frame = 0

Query: 16  SAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           S +D LLPW+  +K  ++ S    +   +L + L  C  +F  + +YKND R++++W   
Sbjct: 23  SGKDHLLPWIRGVK-KMKESLPSQILNEKLPRFLQKCAESFESDKRYKNDSRYIRVWLQL 82

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +   +D     + +  + I    SL Y   A   E   R  DA  +Y LG+   AEP++ 
Sbjct: 83  MDFVDDPRALLRTMEAKSIGTKRSLFYQAYALHYEKMKRFEDAEKMYRLGVQNLAEPMDE 142

Query: 136 LKKAKRLFIDRI 148
           L+K+   F+ R+
Sbjct: 143 LQKSYLQFVTRM 153

BLAST of Pay0005240 vs. TAIR 10
Match: AT2G25090.1 (CBL-interacting protein kinase 16 )

HSP 1 Score: 63.2 bits (152), Expect = 7.5e-10
Identity = 62/268 (23.13%), Postives = 112/268 (41.79%), Query Frame = 0

Query: 228 KYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAK---ER 287
           KY I    GTG FA+VY     S  DDV    I+         M  Q++  +A       
Sbjct: 14  KYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMRLLRH 73

Query: 288 SSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLET 347
            +      +         + +Y+  G L + I+     GK  E++   Y+  +++  ++ 
Sbjct: 74  PNVVELREVMATKKKIFFVMEYVNGGELFEMIDRD---GKLPEDLARKYFQ-QLISAVDF 133

Query: 348 LHSIGIIHGDFKPDNLLVRYAGENLTEDDFQDRRGPWQDQGLCLVDWGRGIDLQLFPENV 407
            HS G+ H D KP+NLL+   G+ L   DF          GL  +    G+  +    + 
Sbjct: 134 CHSRGVFHRDIKPENLLLDGEGD-LKVTDF----------GLSALMMPEGLGGRRGSSDD 193

Query: 408 EFKGDCRTSGFRCPEMLENRPWK-YQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYL 467
                C T  +  PE+L N+ +     DI+   +V++ +L G    +++   +    ++ 
Sbjct: 194 LLHTRCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFK 253

Query: 468 PKSSFKRYWNIELWKNLFTKLLNITPGQ 492
            +  F  ++++E  K L ++LL   P Q
Sbjct: 254 AECEFPPWFSLES-KELLSRLLVPDPEQ 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IVI02.8e-19561.00Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana ... [more]
P416956.1e-4932.65Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (str... [more]
O089012.0e-4732.20Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090... [more]
O436831.7e-4632.03Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 ... [more]
O947512.0e-4431.48Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (st... [more]
Match NameE-valueIdentityDescription
A0A5D3C9K30.0e+0099.44Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003120 P... [more]
A0A1S3C1A00.0e+0099.44mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 ... [more]
A0A5A7SMW70.0e+0099.25Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003080 ... [more]
A0A0A0K5T03.1e-30696.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1[more]
A0A6J1EXJ75.7e-27686.09mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
TYK06996.10.0e+0099.44protein TPX2 [Cucumis melo var. makuwa][more]
XP_008455277.10.0e+0099.44PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo... [more]
KAA0031543.10.0e+0099.25protein TPX2 [Cucumis melo var. makuwa][more]
XP_004136844.16.4e-30696.62mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus] >KGN43... [more]
XP_038888854.13.2e-29793.05mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hi... [more]
Match NameE-valueIdentityDescription
AT2G20635.12.0e-19661.00ATP binding;protein kinases;protein serine/threonine kinases [more]
AT2G33560.23.2e-1328.95BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT2G33560.14.2e-1327.13BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT5G05510.17.2e-1328.79Mad3/BUB1 homology region 1 [more]
AT2G25090.17.5e-1023.13CBL-interacting protein kinase 16 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013212Mad3/Bub1 homology region 1SMARTSM00777mad3_bub1_icoord: 8..126
e-value: 8.2E-11
score: 51.3
IPR013212Mad3/Bub1 homology region 1PFAMPF08311Mad3_BUB1_Icoord: 9..126
e-value: 8.3E-22
score: 77.5
IPR013212Mad3/Bub1 homology region 1PROSITEPS51489BUB1_Ncoord: 6..164
score: 33.369034
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 229..522
e-value: 7.8E-10
score: 5.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 231..448
e-value: 2.4E-17
score: 63.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 229..529
score: 19.128262
NoneNo IPR availableGENE3D1.25.40.430coord: 6..164
e-value: 5.4E-34
score: 119.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 226..529
e-value: 1.4E-99
score: 334.5
NoneNo IPR availablePANTHERPTHR14030:SF4BUB1 KINASE, ISOFORM A-RELATEDcoord: 157..531
coord: 16..149
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 157..531
coord: 16..149
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 350..362
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 226..503

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0005240.1Pay0005240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0051754 meiotic sister chromatid cohesion, centromeric
biological_process GO:0007094 mitotic spindle assembly checkpoint signaling
biological_process GO:0060236 regulation of mitotic spindle organization
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000776 kinetochore
cellular_component GO:0005874 microtubule
cellular_component GO:0005634 nucleus
cellular_component GO:0005819 spindle
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity