Pay0005066 (gene) Melon (Payzawat) v1

Overview


Name	Pay0005066
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	Glutamate synthase
Location	chr06: 8370086 .. 8370782 (-)
RNA-Seq Expression	Pay0005066
Synteny	Pay0005066

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGGCGAAACCTGGTTCTCTTCTTAAACTTCCGGCTGCTCCTTATTCATTTGATAACACCTCTGTTAAGCCTCAATTGAATGTTAACCCCAAAGCTCGTTTAGGAGCGAGAGCTGCTCGATGTTCCGCTTCCAAGGGAGGGTCCGGGTTGTTAAATGTGTCGGAGAAGAAGTTTTTTGGAGCCCGCTTGAGGGCTCCGGGATCCGGAAGATTTCAGTTTTGGCATTTGGATGGTCCTGGCCGGTCACCGAAGCTTCGACTTGCTATCCGGTCGGGTTTGTCGGCTGTGCCTGAGAAACCTCTTGGTCTTTATGATCCTTCGTTCGATAAGGACTCCTCGTGGAGTGTGTTTGTTTGCGAATTATCCGCGAACCAGCGCAAACTCCTAGCCATCATGAGATTTTTTTGTAAATTGTTTTTTTTGTTGGTTTTTTTTTTTTTTTTTTTTAATGTATGGAAATATTGTATGCCATATTAGGGTTGGACTCCTCTTTAACGTGGATATGTATTCGGTATATTGATTTTAAAATTCTTTTTGGATTTAATTTGGGTGGTTTTGCTTTTTGCAGATTACAGATGCTTTGGAAATGTTGGTTCGTATGTCACATAGAGGTGCTTGCGGTTGTGAAACAAATACTGGTGATGGAGCTGGAATTCTTCTTGCTCTTCCACACGAGTTCTTTAAACAGGCATGA

mRNA sequence

ATGCTGGCGAAACCTGGTTCTCTTCTTAAACTTCCGGCTGCTCCTTATTCATTTGATAACACCTCTGTTAAGCCTCAATTGAATGTTAACCCCAAAGCTCGTTTAGGAGCGAGAGCTGCTCGATGTTCCGCTTCCAAGGGAGGGTCCGGGTTGTTAAATGTGTCGGAGAAGAAGTTTTTTGGAGCCCGCTTGAGGGCTCCGGGATCCGGAAGATTTCAGTTTTGGCATTTGGATGGTCCTGGCCGGTCACCGAAGCTTCGACTTGCTATCCGGTCGGGTTTGTCGGCTGTGCCTGAGAAACCTCTTGGTCTTTATGATCCTTCGTTCGATAAGGACTCCTCGTGGAGTGTGTTTGTTTGCGAATTATCCGCGAACCAGCGCAAACTCCTAGCCATCATGAGATTTTTTTGTAAATTGTTTTTTTTGTTGATTACAGATGCTTTGGAAATGTTGGTTCGTATGTCACATAGAGGTGCTTGCGGTTGTGAAACAAATACTGGTGATGGAGCTGGAATTCTTCTTGCTCTTCCACACGAGTTCTTTAAACAGGCATGA

Coding sequence (CDS)

ATGCTGGCGAAACCTGGTTCTCTTCTTAAACTTCCGGCTGCTCCTTATTCATTTGATAACACCTCTGTTAAGCCTCAATTGAATGTTAACCCCAAAGCTCGTTTAGGAGCGAGAGCTGCTCGATGTTCCGCTTCCAAGGGAGGGTCCGGGTTGTTAAATGTGTCGGAGAAGAAGTTTTTTGGAGCCCGCTTGAGGGCTCCGGGATCCGGAAGATTTCAGTTTTGGCATTTGGATGGTCCTGGCCGGTCACCGAAGCTTCGACTTGCTATCCGGTCGGGTTTGTCGGCTGTGCCTGAGAAACCTCTTGGTCTTTATGATCCTTCGTTCGATAAGGACTCCTCGTGGAGTGTGTTTGTTTGCGAATTATCCGCGAACCAGCGCAAACTCCTAGCCATCATGAGATTTTTTTGTAAATTGTTTTTTTTGTTGATTACAGATGCTTTGGAAATGTTGGTTCGTATGTCACATAGAGGTGCTTGCGGTTGTGAAACAAATACTGGTGATGGAGCTGGAATTCTTCTTGCTCTTCCACACGAGTTCTTTAAACAGGCATGA

Protein sequence

MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFFGARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFFKQA

Homology

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BLAST of Pay0005066 vs. ExPASy Swiss-Prot
Match: Q9LV03 (Glutamate synthase 1 [NADH], chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLT1 PE=1 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 2.2e-38
Identity = 85/167 (50.90%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 19  DNTSVKPQLNV-NPKARLGARAARCSASKGGSGLLNVSEKKFFGARLRAPGSGRFQFWHL 78
           ++TSV  QL V +  +R  +  ARCS  K       + E  F G R+R  GS   QFW  
Sbjct: 26  NSTSVASQLAVTSGVSRRRSCTARCSVKKP-----VIPESPFLGTRVRRSGSETLQFWRS 85

Query: 79  DGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFC 138
           DGPGRS KLR  ++S  SAVPEKPLGLYDPS+DKDS    FV ELS    +         
Sbjct: 86  DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTR--------- 145

Query: 139 KLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFFKQA 185
                 +TD+LEML+RM+HRGACGCE+NTGDGAGIL+ LPH+F+ +A
Sbjct: 146 ----KTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEA 174

[Close]

BLAST of Pay0005066 vs. ExPASy Swiss-Prot
Match: Q03460 (Glutamate synthase [NADH], amyloplastic OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-36
Identity = 84/164 (51.22%), Postives = 104/164 (63.41%), Query Frame = 0


Query: 25  PQLNV--NPKARLG--ARAARCSASKGGSGLLNVSEKKFFGARLRAPGS-GRFQFWHLDG 84
           PQ+N   NP ARL   AR  RCSA+        V  K++ G +LR+ G   R Q W   G
Sbjct: 15  PQINAISNPNARLRPLARVTRCSAT-------CVERKRWLGTKLRSGGGLERIQLWESGG 74

Query: 85  PGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFCKL 144
            GR PKLR+A++S  SAVP+KP+GLYDP+FDKDS    FV EL+    +           
Sbjct: 75  LGRLPKLRVAVKSSFSAVPDKPMGLYDPAFDKDSCGVGFVAELNGQSSR----------- 134

Query: 145 FFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFFKQ 184
               +TDALEMLVRM+HRGACGCE NTGDGAGIL+ALPH F+++
Sbjct: 135 --KTVTDALEMLVRMTHRGACGCEANTGDGAGILVALPHGFYQE 158

[Close]

BLAST of Pay0005066 vs. ExPASy Swiss-Prot
Match: Q0JKD0 (Glutamate synthase 1 [NADH], chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0681900 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.6e-20
Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 0


Query: 94  LSAVPEKPLGLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFCKLFFLLITDALEMLVR 153
           LS +PE  +GLYDPS ++DS    FV ELS + ++               + DALEML R
Sbjct: 80  LSTLPESSIGLYDPSRERDSCGVGFVAELSGDYKR-------------ATVNDALEMLER 139

Query: 154 MSHRGACGCETNTGDGAGILLALPHEFFKQ 184
           M+HRGACGCE NTGDGAGIL+ALPH FF++
Sbjct: 140 MAHRGACGCEKNTGDGAGILVALPHNFFRE 156

[Close]

BLAST of Pay0005066 vs. ExPASy Swiss-Prot
Match: Q0DG35 (Glutamate synthase 2 [NADH], chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0555600 PE=2 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 2.1e-20
Identity = 71/191 (37.17%), Postives = 95/191 (49.74%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           M A  G  LKL AAP +     V+ +     K R  A A   +A++   G +++ E  F 
Sbjct: 1   MSAAQGLALKLRAAPAA---GGVRGE-----KRRRAASATAAAAARPRHGAMSL-EGGFL 60

Query: 61  GARL--------RAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKD 120
           G  L        RA  S + +     G  R P         +S +PE  +GLYDPS ++D
Sbjct: 61  GGALPAEDRVAPRASASRQAEAGAGAGAARPPP------RSMSKIPESSIGLYDPSMERD 120

Query: 121 SSWSVFVCELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGI 180
           S    F+ ELS    +               + DA+EML RM+HRGACGCE NTGDGAGI
Sbjct: 121 SCGVGFIAELSGEYSR-------------KTVDDAIEMLDRMAHRGACGCEKNTGDGAGI 163

Query: 181 LLALPHEFFKQ 184
           L+ALPH FF++
Sbjct: 181 LVALPHNFFRE 163

[Close]

BLAST of Pay0005066 vs. ExPASy Swiss-Prot
Match: P55037 (Ferredoxin-dependent glutamate synthase 1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=gltB PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.0e-11
Identity = 32/81 (39.51%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 103 GLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGC 162
           GLYDP  + D+    F+ ++               K+   ++   L+MLV + HRGACGC
Sbjct: 32  GLYDPQNEHDACGVGFIVQMKG-------------KVSHDIVEQGLQMLVNLEHRGACGC 91

Query: 163 ETNTGDGAGILLALPHEFFKQ 184
           E NTGDGAGIL+ +PH+F ++
Sbjct: 92  EPNTGDGAGILIQVPHKFIQK 99

[Close]

BLAST of Pay0005066 vs. ExPASy TrEMBL
Match: A0A5A7UAB6 (Glutamate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00950 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.7e-82
Identity = 159/184 (86.41%), Postives = 160/184 (86.96%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF
Sbjct: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDS    FV 
Sbjct: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF
Sbjct: 121 ELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 171

Query: 181 FKQA 185
           FKQA
Sbjct: 181 FKQA 171

[Close]

BLAST of Pay0005066 vs. ExPASy TrEMBL
Match: A0A5D3BWE5 (Glutamate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00380 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.7e-82
Identity = 159/184 (86.41%), Postives = 160/184 (86.96%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF
Sbjct: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDS    FV 
Sbjct: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF
Sbjct: 121 ELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 171

Query: 181 FKQA 185
           FKQA
Sbjct: 181 FKQA 171

[Close]

BLAST of Pay0005066 vs. ExPASy TrEMBL
Match: A0A1S3B4H4 (glutamate synthase [NADH], amyloplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485701 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.7e-82
Identity = 159/184 (86.41%), Postives = 160/184 (86.96%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF
Sbjct: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDS    FV 
Sbjct: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF
Sbjct: 121 ELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 171

Query: 181 FKQA 185
           FKQA
Sbjct: 181 FKQA 171

[Close]

BLAST of Pay0005066 vs. ExPASy TrEMBL
Match: A0A0A0LDU9 (Glutamine amidotransferase type-2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G603580 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.5e-77
Identity = 153/185 (82.70%), Postives = 157/185 (84.86%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSF-DNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKF 60
           MLAKPGSLLKLPAAPY+F DNTSVKPQLNVNPK RLGARAARCSASKG SGLLNVSEKKF
Sbjct: 1   MLAKPGSLLKLPAAPYTFNDNTSVKPQLNVNPKTRLGARAARCSASKGTSGLLNVSEKKF 60

Query: 61  FGARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFV 120
           FGARLRAPGSGR QFWHLDGPGRSPKLRLA+RSGLS+VPEKPLGLYDPSFDKDS    FV
Sbjct: 61  FGARLRAPGSGRVQFWHLDGPGRSPKLRLAVRSGLSSVPEKPLGLYDPSFDKDSCGVGFV 120

Query: 121 CELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHE 180
            ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHE
Sbjct: 121 AELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHE 172

Query: 181 FFKQA 185
           FFKQA
Sbjct: 181 FFKQA 172

[Close]

BLAST of Pay0005066 vs. ExPASy TrEMBL
Match: A0A6J1FZ98 (glutamate synthase [NADH], amyloplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449255 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.6e-71
Identity = 140/184 (76.09%), Postives = 147/184 (79.89%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAK GSLLKLPAAP++FD TSVKPQLNVNPKARL  RAARCSAS G S  LNVSEKKFF
Sbjct: 1   MLAKSGSLLKLPAAPFTFDKTSVKPQLNVNPKARLATRAARCSASNGASRSLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGR QFWHLDGPGRSPK R A+RSGLSAVPEKPLGLYDP FDKDS    FV 
Sbjct: 61  GARLRAPGSGRVQFWHLDGPGRSPKFRFAVRSGLSAVPEKPLGLYDPKFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRM+HRGACGCE NTGDGAG+LLALPHEF
Sbjct: 121 ELSGESSR-------------KTITDALEMLVRMAHRGACGCEANTGDGAGLLLALPHEF 171

Query: 181 FKQA 185
           FK+A
Sbjct: 181 FKEA 171

[Close]

BLAST of Pay0005066 vs. NCBI nr
Match: TYK03162.1 (glutamate synthase [Cucumis melo var. makuwa])

HSP 1 Score: 312.8 bits (800), Expect = 2.0e-81
Identity = 159/184 (86.41%), Postives = 160/184 (86.96%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF
Sbjct: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDS    FV 
Sbjct: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF
Sbjct: 121 ELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 171

Query: 181 FKQA 185
           FKQA
Sbjct: 181 FKQA 171

[Close]

BLAST of Pay0005066 vs. NCBI nr
Match: KAA0050481.1 (glutamate synthase [Cucumis melo var. makuwa])

HSP 1 Score: 312.8 bits (800), Expect = 2.0e-81
Identity = 159/184 (86.41%), Postives = 160/184 (86.96%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF
Sbjct: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDS    FV 
Sbjct: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF
Sbjct: 121 ELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 171

Query: 181 FKQA 185
           FKQA
Sbjct: 181 FKQA 171

[Close]

BLAST of Pay0005066 vs. NCBI nr
Match: XP_008441619.1 (PREDICTED: glutamate synthase [NADH], amyloplastic isoform X1 [Cucumis melo])

HSP 1 Score: 312.8 bits (800), Expect = 2.0e-81
Identity = 159/184 (86.41%), Postives = 160/184 (86.96%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF
Sbjct: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDS    FV 
Sbjct: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF
Sbjct: 121 ELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 171

Query: 181 FKQA 185
           FKQA
Sbjct: 181 FKQA 171

[Close]

BLAST of Pay0005066 vs. NCBI nr
Match: XP_011651598.1 (glutamate synthase [NADH], amyloplastic isoform X1 [Cucumis sativus] >KGN58271.1 hypothetical protein Csa_017601 [Cucumis sativus])

HSP 1 Score: 298.1 bits (762), Expect = 5.1e-77
Identity = 153/185 (82.70%), Postives = 157/185 (84.86%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSF-DNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKF 60
           MLAKPGSLLKLPAAPY+F DNTSVKPQLNVNPK RLGARAARCSASKG SGLLNVSEKKF
Sbjct: 1   MLAKPGSLLKLPAAPYTFNDNTSVKPQLNVNPKTRLGARAARCSASKGTSGLLNVSEKKF 60

Query: 61  FGARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFV 120
           FGARLRAPGSGR QFWHLDGPGRSPKLRLA+RSGLS+VPEKPLGLYDPSFDKDS    FV
Sbjct: 61  FGARLRAPGSGRVQFWHLDGPGRSPKLRLAVRSGLSSVPEKPLGLYDPSFDKDSCGVGFV 120

Query: 121 CELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHE 180
            ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHE
Sbjct: 121 AELSGETSR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHE 172

Query: 181 FFKQA 185
           FFKQA
Sbjct: 181 FFKQA 172

[Close]

BLAST of Pay0005066 vs. NCBI nr
Match: XP_038893053.1 (glutamate synthase [NADH], amyloplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 294.3 bits (752), Expect = 7.4e-76
Identity = 150/184 (81.52%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 1   MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60
           MLAKPGSLLKLPAAP +FDNTSVK QLNVNPKARLGARAARCSASKG S LLNVSEKKFF
Sbjct: 1   MLAKPGSLLKLPAAPSTFDNTSVKTQLNVNPKARLGARAARCSASKGASRLLNVSEKKFF 60

Query: 61  GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVC 120
           GARLRAPGSGR QFWHLDGPGRSPKLRL +RSGLSAVPEKPLGLYDPSFDKDS    FV 
Sbjct: 61  GARLRAPGSGRVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121 ELSANQRKLLAIMRFFCKLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 180
           ELS    +               ITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF
Sbjct: 121 ELSGETNR-------------KTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEF 171

Query: 181 FKQA 185
           FK+A
Sbjct: 181 FKEA 171

[Close]

BLAST of Pay0005066 vs. TAIR 10
Match: AT5G53460.1 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 160.2 bits (404), Expect = 1.6e-39
Identity = 85/167 (50.90%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 19  DNTSVKPQLNV-NPKARLGARAARCSASKGGSGLLNVSEKKFFGARLRAPGSGRFQFWHL 78
           ++TSV  QL V +  +R  +  ARCS  K       + E  F G R+R  GS   QFW  
Sbjct: 26  NSTSVASQLAVTSGVSRRRSCTARCSVKKP-----VIPESPFLGTRVRRSGSETLQFWRS 85

Query: 79  DGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFC 138
           DGPGRS KLR  ++S  SAVPEKPLGLYDPS+DKDS    FV ELS    +         
Sbjct: 86  DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTR--------- 145

Query: 139 KLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFFKQA 185
                 +TD+LEML+RM+HRGACGCE+NTGDGAGIL+ LPH+F+ +A
Sbjct: 146 ----KTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEA 174

[Close]

BLAST of Pay0005066 vs. TAIR 10
Match: AT5G53460.2 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 160.2 bits (404), Expect = 1.6e-39
Identity = 85/167 (50.90%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 19  DNTSVKPQLNV-NPKARLGARAARCSASKGGSGLLNVSEKKFFGARLRAPGSGRFQFWHL 78
           ++TSV  QL V +  +R  +  ARCS  K       + E  F G R+R  GS   QFW  
Sbjct: 26  NSTSVASQLAVTSGVSRRRSCTARCSVKKP-----VIPESPFLGTRVRRSGSETLQFWRS 85

Query: 79  DGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFC 138
           DGPGRS KLR  ++S  SAVPEKPLGLYDPS+DKDS    FV ELS    +         
Sbjct: 86  DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTR--------- 145

Query: 139 KLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFFKQA 185
                 +TD+LEML+RM+HRGACGCE+NTGDGAGIL+ LPH+F+ +A
Sbjct: 146 ----KTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEA 174

[Close]

BLAST of Pay0005066 vs. TAIR 10
Match: AT5G53460.3 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 160.2 bits (404), Expect = 1.6e-39
Identity = 85/167 (50.90%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 19  DNTSVKPQLNV-NPKARLGARAARCSASKGGSGLLNVSEKKFFGARLRAPGSGRFQFWHL 78
           ++TSV  QL V +  +R  +  ARCS  K       + E  F G R+R  GS   QFW  
Sbjct: 26  NSTSVASQLAVTSGVSRRRSCTARCSVKKP-----VIPESPFLGTRVRRSGSETLQFWRS 85

Query: 79  DGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSSWSVFVCELSANQRKLLAIMRFFC 138
           DGPGRS KLR  ++S  SAVPEKPLGLYDPS+DKDS    FV ELS    +         
Sbjct: 86  DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTR--------- 145

Query: 139 KLFFLLITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFFKQA 185
                 +TD+LEML+RM+HRGACGCE+NTGDGAGIL+ LPH+F+ +A
Sbjct: 146 ----KTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEA 174

[Close]

BLAST of Pay0005066 vs. TAIR 10
Match: AT5G04140.1 (glutamate synthase 1 )

HSP 1 Score: 46.6 bits (109), Expect = 2.5e-05
Identity = 17/39 (43.59%), Postives = 28/39 (71.79%), Query Frame = 0


Query: 143 LITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFF 182
           ++ DAL  L  M HRG CG + ++GDG+G++ ++P +FF
Sbjct: 122 VVKDALIALGCMEHRGGCGADNDSGDGSGLMSSIPWDFF 160

[Close]

BLAST of Pay0005066 vs. TAIR 10
Match: AT5G04140.2 (glutamate synthase 1 )

HSP 1 Score: 46.6 bits (109), Expect = 2.5e-05
Identity = 17/39 (43.59%), Postives = 28/39 (71.79%), Query Frame = 0


Query: 143 LITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFF 182
           ++ DAL  L  M HRG CG + ++GDG+G++ ++P +FF
Sbjct: 148 VVKDALIALGCMEHRGGCGADNDSGDGSGLMSSIPWDFF 186

The following BLAST results are available for this feature:

BLAST of Pay0005066 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LV03	2.2e-38	50.90	Glutamate synthase 1 [NADH], chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GL...	[more]
Q03460	1.6e-36	51.22	Glutamate synthase [NADH], amyloplastic OS=Medicago sativa OX=3879 PE=1 SV=1	[more]
Q0JKD0	1.6e-20	53.33	Glutamate synthase 1 [NADH], chloroplastic OS=Oryza sativa subsp. japonica OX=39...	[more]
Q0DG35	2.1e-20	37.17	Glutamate synthase 2 [NADH], chloroplastic OS=Oryza sativa subsp. japonica OX=39...	[more]
P55037	3.0e-11	39.51	Ferredoxin-dependent glutamate synthase 1 OS=Synechocystis sp. (strain PCC 6803 ...	[more]

BLAST of Pay0005066 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UAB6	9.7e-82	86.41	Glutamate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G0...	[more]
A0A5D3BWE5	9.7e-82	86.41	Glutamate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00...	[more]
A0A1S3B4H4	9.7e-82	86.41	glutamate synthase [NADH], amyloplastic isoform X1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LDU9	2.5e-77	82.70	Glutamine amidotransferase type-2 domain-containing protein OS=Cucumis sativus O...	[more]
A0A6J1FZ98	1.6e-71	76.09	glutamate synthase [NADH], amyloplastic-like isoform X2 OS=Cucurbita moschata OX...	[more]

BLAST of Pay0005066 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK03162.1	2.0e-81	86.41	glutamate synthase [Cucumis melo var. makuwa]	[more]
KAA0050481.1	2.0e-81	86.41	glutamate synthase [Cucumis melo var. makuwa]	[more]
XP_008441619.1	2.0e-81	86.41	PREDICTED: glutamate synthase [NADH], amyloplastic isoform X1 [Cucumis melo]	[more]
XP_011651598.1	5.1e-77	82.70	glutamate synthase [NADH], amyloplastic isoform X1 [Cucumis sativus] >KGN58271.1...	[more]
XP_038893053.1	7.4e-76	81.52	glutamate synthase [NADH], amyloplastic isoform X1 [Benincasa hispida]	[more]

BLAST of Pay0005066 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53460.1	1.6e-39	50.90	NADH-dependent glutamate synthase 1	[more]
AT5G53460.2	1.6e-39	50.90	NADH-dependent glutamate synthase 1	[more]
AT5G53460.3	1.6e-39	50.90	NADH-dependent glutamate synthase 1	[more]
AT5G04140.1	2.5e-05	43.59	glutamate synthase 1	[more]
AT5G04140.2	2.5e-05	43.59	glutamate synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017932	Glutamine amidotransferase type 2 domain	PFAM	PF00310	GATase_2	coord: 143..183 e-value: 8.4E-14 score: 51.1
IPR029055	Nucleophile aminohydrolases, N-terminal	GENE3D	3.60.20.10	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	coord: 141..184 e-value: 7.9E-16 score: 59.7
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 139..183
None	No IPR available	PANTHER	PTHR11938	FAD NADPH DEHYDROGENASE/OXIDOREDUCTASE	coord: 89..183
None	No IPR available	PANTHER	PTHR11938:SF139	GLUTAMATE SYNTHASE 1 [NADH], CHLOROPLASTIC	coord: 89..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0005066.1	Pay0005066.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019676	ammonia assimilation cycle
biological_process	GO:0097054	L-glutamate biosynthetic process
molecular_function	GO:0051538	3 iron, 4 sulfur cluster binding
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0016040	glutamate synthase (NADH) activity
molecular_function	GO:0005506	iron ion binding