Pay0004422 (gene) Melon (Payzawat) v1

BLAST of Pay0004422 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 9.7e-161
Identity = 308/518 (59.46%), Postives = 394/518 (76.06%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLM---AILSSYGSCS---FHTKLSIEEATLIDLQHAFYQNKLTSR 60
           M    F  + S+L+ L+   A++ S GS S     +  SI+EAT+ D++ AF + +LTS+
Sbjct: 1   MVALRFFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSK 60

Query: 61  QLVEFYLEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNI 120
           QLVE YLE + + NPIL  +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I
Sbjct: 61  QLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 120

Query: 121 ATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSA 180
           +TKDKLNTTAGSFALLGS+V RDAGVV +LR +GA+I GKASLSEW++FRS  +P GWSA
Sbjct: 121 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 180

Query: 181 RGGQGKNPYIL-GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVG 240
           RG QGKNPY+L   P GSSSGSAISV AN+V VSLGTETDGSIL P++ NSVVGIKP+VG
Sbjct: 181 RGLQGKNPYVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVG 240

Query: 241 LTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFL 300
           LTSRAGV+PIS RQDS+GPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL
Sbjct: 241 LTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFL 300

Query: 301 RAGGLKGKRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGS 360
              GLKGKR+GIV +          +    +  +KTL++ GAI+++N TI N+++I  G+
Sbjct: 301 TTSGLKGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGT 360

Query: 361 -SGEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEA 420
            SGE+IALLAEFK+SLNAYLKELV SP+RSL+D I +N++ ++ E + E+GQE+FL AEA
Sbjct: 361 DSGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEA 420

Query: 421 TNGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGY 480
           T+G+G+ EK AL ++ +LS++G E+L+ +NKLDAI   GS +SSV AIGG+PG +VPAGY
Sbjct: 421 TSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGY 480

Query: 481 DPQGVPYGITFGGLKGFEPRLIEIAYGFEHLTKSRKPP 510
           D  GVPYGI+FGGL+  EP+LIEIA+ FE  T  RKPP
Sbjct: 481 DSGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of Pay0004422 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.4e-79
Identity = 204/512 (39.84%), Postives = 288/512 (56.25%), Query Frame = 0

Query: 32  LSIEEATLIDLQHAFYQNKLTSRQLVEFYLEQVHRFNPILKGIIEVNPDALDQASRADIE 91
           +++E+AT+  LQ+      LTS  +V  YL++  + NP + GI+++NPD L  AS  D E
Sbjct: 64  ITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDE 123

Query: 92  RKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAII 151
           R     R   PLHGIP +VKDN ATKDK++TTAGS+ALLGSIVPRDA VV +LR AGA++
Sbjct: 124 RANGIIR--GPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVL 183

Query: 152 FGKASLSEWSYFRSNELPSGWSARGGQGKNPYILG-EPCGSSSGSAISVAANMVTVSLGT 211
           FG A+LSEW+  RSN+   G+SARGGQ + P+ L   P GSSSGSAISVA+NM+  +LGT
Sbjct: 184 FGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGT 243

Query: 212 ETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGA 271
           ETDGSI+ P+  N VVG+KPTVGLTSR GVIP S  QD+ GPI RTV DA YV  ++ G 
Sbjct: 244 ETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGI 303

Query: 272 DRYDNSTIEASKYIPK-GGYGQFL-RAGGLKGKRIGI--VREFYDFGHDETFYPQAYEKV 331
           D  D  T+  +   P+ G Y +FL     L+G R G+   R + +   DE        +V
Sbjct: 304 DENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEI---DRLLEV 363

Query: 332 LKTLKKGGAILVDNRTIDNLQLIF-DG--------SSGEQIALLAEFKISLNAYLKELVA 391
           +K +++ GAI+ +N    NL +I  DG        +  E   +  +F  ++ +YL E+  
Sbjct: 364 VKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKN 423

Query: 392 SPIRSLSDAIEFNKKNSKLEN--------LEYGQELFLKAEATNGI-GDAEKAALARLAK 451
           + I SL D +E+N K    E            GQ+ FL +    G+  +    A+  + +
Sbjct: 424 TEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRR 483

Query: 452 LSKD-GFERLM---IKNKLDAIAAPGSLISSVFAI-------GGFPGFSVPAGYDPQGVP 510
            S+D G +  +        D+    G L+ S  +I        G+P  ++P G    G P
Sbjct: 484 TSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRP 543

BLAST of Pay0004422 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.4e-68
Identity = 180/490 (36.73%), Postives = 256/490 (52.24%), Query Frame = 0

Query: 40  IDLQHAFYQNKLTSRQLVEFYLEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRS 99
           I LQ  + Q  +    +V+ Y+ ++   N  ++ + E+NPDAL  A + D ERK    R 
Sbjct: 92  ITLQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLR- 151

Query: 100 LSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSE 159
             PLHG+P+++K+NI T DK+++TAGS+A+ G+    DA V TKLR AG +I GK+  S+
Sbjct: 152 -GPLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQ 211

Query: 160 WSYFRSNELPSGWSARGGQGKNPYILG-EPCGSSSGSAISVAANMVTVSLGTETDGSILC 219
           W+ FRS    +GWSA GGQ    YI   +P GSSSGS ++    +   +LGTET GSI+ 
Sbjct: 212 WANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVS 271

Query: 220 PSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTI 279
           P+  +++VG+KPTVGLTSR  V+PIS RQD+VGP+ R+V DA Y+L  I G D  DN T 
Sbjct: 272 PADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT- 331

Query: 280 EASKYIPKGGYGQFLRAGGLKGKRIGIVREFYD-FGHDETFYPQAYEKVLKTLKKGGAIL 339
            A  +     Y +      LKGKRIG+ R     FG  +T   Q + + L  +KK GAI+
Sbjct: 332 SAIPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQ-FNQALAVMKKAGAII 391

Query: 340 VDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASP--IRSLSDAIEFNKKNSK 399
           V+N    +          + I L A+   +L A+ K+L  +P  I  L     F  ++ +
Sbjct: 392 VENTDFTSFAEFAQSPIPDDI-LYADSLTNLPAFFKQLKVNPHNITDLESLRRFT-QHHR 451

Query: 400 LENLEYGQELFLKAEATNGIGDAEKAALARLAKLSKDGFERLMI----KNKLDAIAAPGS 459
           LE                GI + +        K  K G E  ++    ++KLDA   P  
Sbjct: 452 LEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTD 511

Query: 460 LISSVFAIGGFPGFSVPAGYDPQ---------------GVPYGITFGGLKGFEPRLIEIA 507
           L   + A+ G P  +VP G  P                G+P GI F G    E +LI +A
Sbjct: 512 LSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLA 571

BLAST of Pay0004422 vs. ExPASy Swiss-Prot
Match: Q8RC40 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=gatA PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.7e-43
Identity = 149/508 (29.33%), Postives = 251/508 (49.41%), Query Frame = 0

Query: 32  LSIEEATLIDLQHAFYQNKLTSRQLVEFYLEQVHRFNPILKGIIEVNPD-ALDQASRADI 91
           + +   T+ +L+   ++ +++++++ + YLE++    P +  ++ +  + AL++A  AD 
Sbjct: 1   MELYSLTVHELKELLHKREVSAQEVTKSYLERIKEVEPKIDALVTITEEFALERAKVADE 60

Query: 92  ERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAI 151
             K    + L+   GIPV++KDNI+T + + TT  S  L   I P +A VV +L   G +
Sbjct: 61  MIKNGEAKDLT---GIPVIIKDNIST-EGIRTTCSSKMLENYIPPYNATVVERLLNEGVV 120

Query: 152 IFGKASLSEWSYFRSNELPSGWSARGGQGKNPYILGE-PCGSSSGSAISVAANMVTVSLG 211
           I GK++L E++   S E     ++     KNP+ L   P GSS GSA +VAA+    +LG
Sbjct: 121 ILGKSNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAVAADEAAFALG 180

Query: 212 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVG 271
           ++T GSI  P++L  VVG+KPT GL SR G++  +S  D +GP  + V+D   VL+AI G
Sbjct: 181 SDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNAIAG 240

Query: 272 ADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGHDETFYPQAYEKVLKT 331
            D  D+++++    I K  Y  +L+   +KG RIG+ +EF+  G +E    +  EK +K 
Sbjct: 241 HDPMDSTSVK----IEKPDYTSYLKE-DIKGLRIGVAKEFFGAGIEEG-VKETVEKAIKV 300

Query: 332 LKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIE- 391
            +  GA ++D     ++  +        I   AE   +L  Y         ++  D ++ 
Sbjct: 301 FEDLGAEIID----ISVPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAKNYEDLVDM 360

Query: 392 FNKKNSKLENLEYGQELFLKAEA-TNGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIA 451
           +    S+    E  + + L   A ++G  DA      ++  L K+ FER     K D I 
Sbjct: 361 YMTSRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFERAF--EKCDVIV 420

Query: 452 APGS-----------------LISSVFA----IGGFPGFSVPAGYDPQGVPYGITFGGLK 511
            P S                  ++ ++     I G P  S+P G   +G+P G+   G  
Sbjct: 421 GPTSPTVAFKIGERTNDPLAMYLADIYTVSVNIAGLPAISIPCGLS-EGLPVGLQIIGKH 480

Query: 512 GFEPRLIEIAYGFEHLTK-SRKPPSING 514
             E R++ +AY FE   K   KP +I G
Sbjct: 481 FDEGRILNVAYAFEKAYKFDAKPQAIGG 486

BLAST of Pay0004422 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.7e-43
Identity = 151/508 (29.72%), Postives = 250/508 (49.21%), Query Frame = 0

Query: 32  LSIEEATLIDLQHAFYQNKLTSRQLVEFYLEQVHRFNPILKGIIEVNPD-ALDQASRADI 91
           + +   T+ +L+    + ++++ ++ + YLE++    P +  ++ +  D AL +A  AD 
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADE 60

Query: 92  ERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAI 151
           + KK    +L+   GIPV++KDNI+T + + TT  S  L   I P +A VV KL   G I
Sbjct: 61  KIKKGEDTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVI 120

Query: 152 IFGKASLSEWSYFRSNELPSGWSARGGQGKNPYILGE-PCGSSSGSAISVAANMVTVSLG 211
           I GK++L E++   S E     ++     KNP+ L   P GSS GSA ++AA+    +LG
Sbjct: 121 ILGKSNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALG 180

Query: 212 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVG 271
           ++T GSI  P++L  VVG+KPT GL SR G++  +S  D +GP  + V+D   VL+ I+G
Sbjct: 181 SDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIG 240

Query: 272 ADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGHDETFYPQAYEKVLKT 331
            D  D+++++    I K  Y  +L+   +KG RIG+ +EF+  G +E    +  ++ +K 
Sbjct: 241 HDPKDSTSLK----IDKPDYTSYLKE-DIKGLRIGVAKEFFGEGIEEG-VKETVQESIKV 300

Query: 332 LKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSDAIE- 391
           L+  GA ++D     ++  +        I   AE   +L  Y             D I+ 
Sbjct: 301 LQDLGAEIID----ISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDM 360

Query: 392 FNKKNSKLENLEYGQELFLKAEA-TNGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIA 451
           +    S+    E  + + L   A ++G  DA      ++  L K+ FE+     K D I 
Sbjct: 361 YMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVII 420

Query: 452 APGS-----------------LISSVFA----IGGFPGFSVPAGYDPQGVPYGITFGGLK 511
            P S                  ++ ++     I G PG S+P G    G+P G+   G  
Sbjct: 421 GPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVGLQIIGRH 480

Query: 512 GFEPRLIEIAYGFEHLTK-SRKPPSING 514
             E +++ +AY FE   K S KP +I G
Sbjct: 481 FDEGKILNVAYAFEQANKFSAKPQAIGG 486

BLAST of Pay0004422 vs. ExPASy TrEMBL
Match: A0A5D3BKT1 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001390 PE=4 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.3e-285
Identity = 512/514 (99.61%), Postives = 512/514 (99.61%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV RFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYGI 480
           AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPG SVPAGYDPQGVPYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 515
           TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 514

BLAST of Pay0004422 vs. ExPASy TrEMBL
Match: E5GC09 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.3e-285
Identity = 512/514 (99.61%), Postives = 512/514 (99.61%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV RFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYGI 480
           AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPG SVPAGYDPQGVPYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 515
           TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 514

BLAST of Pay0004422 vs. ExPASy TrEMBL
Match: A0A1S3C5K8 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.3e-285
Identity = 512/514 (99.61%), Postives = 512/514 (99.61%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV RFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYGI 480
           AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPG SVPAGYDPQGVPYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 515
           TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 514

BLAST of Pay0004422 vs. ExPASy TrEMBL
Match: A0A0A0LM97 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=4 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.5e-265
Identity = 478/511 (93.54%), Postives = 491/511 (96.09%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MAGQSFLL +SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV R NPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAILVDN  IDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQE FLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYGI 480
           AALARLAKLSKDGFERLMIKNKLDA+AAPGSLIS VFAIGGFPG SVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

Query: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 512
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 511

BLAST of Pay0004422 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 6.0e-238
Identity = 431/512 (84.18%), Postives = 463/512 (90.43%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MA QSF +Y+SMLLGL+AILSSYGSCSF T  SIEEATL DLQ AFYQNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV RFNPIL GIIEVNPDAL+QAS+AD+ERK+SSPRSLSPLHGIPV VKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVFVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLSEWS FRS E P+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PIS RQD+VGPICRTV+DA YVLDAI G DRYDNSTIEASKY+PKGGYGQFL+  GLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVR+ YDFGHD+ FY  A+EKV KTLK+GGAILVDN TI++  +I   SSGE  A+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAE 420
           LAEFKIS+N YLK+LVASPIRSLSDAIEFN+KNSKLE L EYGQELFL+AEAT GIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYG 480
           KAALARLAKLSK+GFERLMIKNKLDAIAAPG LIS   AIGGFPG SVPAGY+PQG+P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 ITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 512
           I FGGLKGFEPRLIEIAYGFEHLT  RK PS+
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSL 512

BLAST of Pay0004422 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 991.5 bits (2562), Expect = 2.7e-285
Identity = 512/514 (99.61%), Postives = 512/514 (99.61%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV RFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYGI 480
           AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPG SVPAGYDPQGVPYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

Query: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 515
           TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL
Sbjct: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 514

BLAST of Pay0004422 vs. NCBI nr
Match: XP_004147023.1 (probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Csa_006678 [Cucumis sativus])

HSP 1 Score: 924.9 bits (2389), Expect = 3.1e-265
Identity = 478/511 (93.54%), Postives = 491/511 (96.09%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MAGQSFLL +SMLLGLMAILSS GSCSFHT+LS+EEATL DLQ AFYQNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV R NPILKGIIEVNPDALDQASRAD+ERKKSSPRSLSPLHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSN LPSGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PISSRQD+VGPICRTVSDA YVL+AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVREFYDFG D+TFY QAYEKV+KTLKKGGAILVDN  IDNL+ IFDGSSGEQIAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420
           LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQE FLKAEATNGIGDAEK
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYGI 480
           AALARLAKLSKDGFERLMIKNKLDA+AAPGSLIS VFAIGGFPG SVPAGYDPQG PYGI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

Query: 481 TFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 512
            FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 CFGGLKGFEPRLIEIAYGFERLTKSRKPPSI 511

BLAST of Pay0004422 vs. NCBI nr
Match: XP_038900912.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 839.7 bits (2168), Expect = 1.3e-239
Identity = 433/512 (84.57%), Postives = 468/512 (91.41%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MA QSF LY+SMLL L+AILSSYGSCSF T LSIEEAT+ DLQ AFYQNKL SRQLV+FY
Sbjct: 6   MAAQSFWLYISMLLSLLAILSSYGSCSFDTHLSIEEATVKDLQLAFYQNKLISRQLVKFY 65

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           L  V RFNPILKGIIEVNPDALDQAS+AD+ERK++SP SLSPLHGIPVLVKDNIAT+DKL
Sbjct: 66  LNXVRRFNPILKGIIEVNPDALDQASQADLERKRNSPSSLSPLHGIPVLVKDNIATRDKL 125

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLS+WSYFRS  +PSGWSARGGQGK
Sbjct: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSKWSYFRSYXIPSGWSARGGQGK 185

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSS GSAIS+AANMV VSLGTETDGSILCPS LNSVVGIKPTVGLTSRAGV
Sbjct: 186 NPYTMGEPCGSSGGSAISIAANMVAVSLGTETDGSILCPSILNSVVGIKPTVGLTSRAGV 245

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           IPIS RQD+VGPICRTVSD  YVL AIVGAD+ DNSTIEASKYIP+GGY QFLRA GLKG
Sbjct: 246 IPISLRQDTVGPICRTVSDDAYVLGAIVGADKNDNSTIEASKYIPRGGYAQFLRADGLKG 305

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVR+F+DFGHD+ FYP+AYEKV KTL++GGA+ VDN TID+LQ+I   SSGE+ AL
Sbjct: 306 KRIGIVRKFFDFGHDDAFYPRAYEKVFKTLRQGGAVFVDNLTIDSLQVIMGSSSGEETAL 365

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAE 420
           LAEFKISLNAYLK+LVASPIRSLSDAIEFN+KNSKLE L EYGQ LF++AEAT GIGDAE
Sbjct: 366 LAEFKISLNAYLKQLVASPIRSLSDAIEFNEKNSKLEKLREYGQNLFIEAEATKGIGDAE 425

Query: 421 KAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYG 480
           KAALARLAKLSKDGFER+M+KNKLDAIAAPG  ISSV AIGGFPG SVPAGY+ QGVP+G
Sbjct: 426 KAALARLAKLSKDGFERVMLKNKLDAIAAPGWEISSVLAIGGFPGISVPAGYNSQGVPFG 485

Query: 481 ITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 512
           I+FGGLKGFEPRLIEIAYGFEHLTKSRK PSI
Sbjct: 486 ISFGGLKGFEPRLIEIAYGFEHLTKSRKSPSI 517

BLAST of Pay0004422 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 834.7 bits (2155), Expect = 4.3e-238
Identity = 432/512 (84.38%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60
           MA QSF +Y+SMLLGL+AILSSYGSCSF T  SIEEATL DLQ AFYQNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120
           LEQV RFNPIL GIIEVNPDAL+QAS+AD+ERK+SSPRSLSPLHGIPVLVKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLSEWS FRS E P+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300
           +PIS RQD+VGPICRTV+DA YVLDAI G DRYDNSTIEASKY+PKGGYGQFL+  GLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360
           KRIGIVR+ YDFGHD+ FY  A+EKV KTLK+GGAILVDN TI++  +I   SSGE  A+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAE 420
           LAEFKIS+N YLK+LVASPIRSLSDAIEFN+KNSKLE L EYGQELFL+AEAT GIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYG 480
           KAALARLAKLSK+GFERLMIKNKLDAIAAPG LIS   AIGGFPG SVPAGY+PQG+P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 ITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 512
           I FGGLKGFEPRLIEIAYGFEHLT  RK PS+
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSL 512

BLAST of Pay0004422 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 829.7 bits (2142), Expect = 1.4e-236
Identity = 436/514 (84.82%), Postives = 464/514 (90.27%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLMAILSSY--GSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVE 60
           MA QSF +Y+SMLLGL+AILS Y  GSCSF T  SIEEATL D Q AFYQNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKD 120
           FYLEQV R NPILKGIIEVNPDAL+QAS+AD++RK+SS RSLSPLHGIPVLVKDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLSEWS FRS E P+GWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPY +GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGL 300
           GV+PIS RQD+VGPICRTV+DA YVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQI 360
           KGKRIGIVR+ YDFGHD+ FY  A+EKV KTLK+GGAILVDN TI+   +I   SSGE  
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLE L EYGQELFL+AEAT GIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVP 480
           AEKAALARLAKLSK+GFERLMIKNKLDAIAAPG LIS   AIGGFPG SVPAGY+PQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 YGITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 512
           +GI FGGLKGF+PRLIEIAYGFEHLT  RK PS+
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSL 514

BLAST of Pay0004422 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 486.1 bits (1250), Expect = 3.5e-137
Identity = 274/517 (53.00%), Postives = 356/517 (68.86%), Query Frame = 0

Query: 1   MAGQSFLLYVSMLLGLM---AILSSYGSCS---FHTKLSIEEATLIDLQHAFYQNKLTSR 60
           M    F  + S+L+ L+   A++ S GS S     +  SI+EAT+ D++ AF + +LTS+
Sbjct: 1   MVALRFFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSK 60

Query: 61  QLVEFYLEQVHRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNI 120
           QLVE YLE + + NPIL  +IE NPDAL QA  AD ER   +   L  LHG+PVL+KD+I
Sbjct: 61  QLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 120

Query: 121 ATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSA 180
           +TKDKLNTTAGSFALLGS+V RDAGVV +LR +GA+I GKASLSEW++FRS  +P GWSA
Sbjct: 121 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 180

Query: 181 RGGQGKNPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240
                                                       PS  NSVVGIKP+VGL
Sbjct: 181 --------------------------------------------PSQ-NSVVGIKPSVGL 240

Query: 241 TSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLR 300
           TSRAGV+PIS RQDS+GPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL 
Sbjct: 241 TSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLT 300

Query: 301 AGGLKGKRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGS- 360
             GLKGKR+GIV +          +    +  +KTL++ GAI+++N TI N+++I  G+ 
Sbjct: 301 TSGLKGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTD 360

Query: 361 SGEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEAT 420
           SGE+IALLAEFK+SLNAYLKELV SP+RSL+D I +N++ ++ E + E+GQE+FL AEAT
Sbjct: 361 SGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEAT 420

Query: 421 NGIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYD 480
           +G+G+ EK AL ++ +LS++G E+L+ +NKLDAI   GS +SSV AIGG+PG +VPAGYD
Sbjct: 421 SGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYD 462

Query: 481 PQGVPYGITFGGLKGFEPRLIEIAYGFEHLTKSRKPP 510
             GVPYGI+FGGL+  EP+LIEIA+ FE  T  RKPP
Sbjct: 481 SGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of Pay0004422 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 129.8 bits (325), Expect = 6.3e-30
Identity = 141/520 (27.12%), Postives = 237/520 (45.58%), Query Frame = 0

Query: 19  ILSSYGSCSFHTK-LSIEEATLIDLQHAFYQNKLTSRQLVEFYLEQVHRFNPILKGIIEV 78
           I+S+  S +  T  +S  ++ ++  + +    + T+ ++ + YL ++    P LK  + V
Sbjct: 25  IVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHV 84

Query: 79  NPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRD 138
           + + L  A   +I+++ +    L PL G+ + VKDNI T+  + +TA S  L     P D
Sbjct: 85  SENVLKDAQ--EIDQRIAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFD 144

Query: 139 AGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGKNPYILGE-PCGSSSGSA 198
           A  V K++  G I+ GK ++ E+    + E  S +        NP+ L   P GSS GSA
Sbjct: 145 ATAVKKIKELGGIVVGKTNMDEFGMGSTTE-ASAFQVTA----NPWDLSRVPGGSSGGSA 204

Query: 199 ISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRT 258
            +VAA    VSLG++T GS+  P++   VVG+KPT G  SR G++  +S  D +G    T
Sbjct: 205 AAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGST 264

Query: 259 VSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFL-----RAGGLKGKRIGIVREFYD 318
           V+DA  +L AI G DR+D++   +SK        QFL      +  L G ++GI+RE  +
Sbjct: 265 VADAGMLLHAISGYDRFDST---SSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRETLE 324

Query: 319 FGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAY 378
            G D +    A ++    L+  G IL    T  +L     G     +   +E   +L+ Y
Sbjct: 325 DGVD-SGVRSATQEAASHLEALGCIL----TEVSLPSFSLGLPAYYVIASSESSSNLSRY 384

Query: 379 LKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKA-----EATNGIGDAEKAALARL 438
                 + +     A E NK         +G E+ ++        + G  DA      ++
Sbjct: 385 DGVRYGNQVM----AEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYDAYYKRAQQV 444

Query: 439 AKLSKDGFERLMIKNK-LDAIAAP------------------GSLISSVFAIGGFPGFSV 498
             L +  F+  + +N  L + AAP                  G +++    + G P   +
Sbjct: 445 RTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVL 504

Query: 499 PAGY---DPQGVPYGITFGGLKGFEPRLIEIAYGFEHLTK 505
           P G     P G+P G+   G    E +L+++ + FE   K
Sbjct: 505 PCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLK 524

BLAST of Pay0004422 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-27
Identity = 83/244 (34.02%), Postives = 129/244 (52.87%), Query Frame = 0

Query: 38  TLIDLQHAFYQNKLTSRQLVEFYLEQVHRFNPILKGIIEVNPD-ALDQASRADIERKKSS 97
           ++++L       ++TS++LV  YL+Q+ R+N +L+ ++    + A  QA  AD     S 
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQ 263

Query: 98  PRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKAS 157
              L PLHGIP  +KD +A      TT GS +     +  +A V  +L+ +GA++  K  
Sbjct: 264 GTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLV 323

Query: 158 LSEWSYFRSNELPSGWSARGGQGKNPYILGE-PCGSSSGSAISVAANMVTVSLGTETDGS 217
               +Y         W   GG+ +NP+ + E   GSS+G A S +A MV  ++G+ET GS
Sbjct: 324 TGSMAY------DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGS 383

Query: 218 ILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDN 277
           +  P+    +  ++PT G   R GV+ IS   D +GP CRT +D   +LDAI G D  D 
Sbjct: 384 MTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDL 436

Query: 278 STIE 280
           S+ E
Sbjct: 444 SSRE 436

BLAST of Pay0004422 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 112.1 bits (279), Expect = 1.4e-24
Identity = 81/244 (33.20%), Postives = 125/244 (51.23%), Query Frame = 0

Query: 38  TLIDLQHAFYQNKLTSRQLVEFYLEQVHRFNPILKGIIEVNPD-ALDQASRADIERKKSS 97
           ++++L       ++TS++LV  YL+Q+ R+N +L+ ++    + A  QA  AD     S 
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQ 263

Query: 98  PRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKAS 157
              L PLHGIP  +KD +A      TT GS +     +  +A V  +L+ +GA++  K  
Sbjct: 264 GTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLV 323

Query: 158 LSEWSYFRSNELPSGWSARGGQGKNPYILGE-PCGSSSGSAISVAANMVTVSLGTETDGS 217
               +Y         W   GG+ +NP+ + E   GSS+G A S +A       G+ET GS
Sbjct: 324 TGSMAY------DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGS 383

Query: 218 ILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDN 277
           +  P+    +  ++PT G   R GV+ IS   D +GP CRT +D   +LDAI G D  D 
Sbjct: 384 MTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDL 429

Query: 278 STIE 280
           S+ E
Sbjct: 444 SSRE 429

BLAST of Pay0004422 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 97.4 bits (241), Expect = 3.5e-20
Identity = 117/494 (23.68%), Postives = 202/494 (40.89%), Query Frame = 0

Query: 41  DLQHAFYQNKLTSRQLVEFYLEQVHRF---NPILKGIIEVNPDALDQASRADIERKKSSP 100
           D  +A+     T  Q+ +  +  +  F    P    +I  + + + + + A   R +   
Sbjct: 133 DYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQG- 192

Query: 101 RSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASL 160
             +S L GI V +KD+I           ++      V +D+ VV+KLR  GAI+ GKA++
Sbjct: 193 NPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANM 252

Query: 161 SEWSYFRSNELPSGWSARGGQGKNPYILGEP----CGSSSGSAISVAANMVTVSLGTETD 220
            E          +G ++  G  +NP+   +P     GSSSGSA  VAA + + +LGT+  
Sbjct: 253 HELGMG-----TTGNNSNYGTTRNPH---DPKRYTGGSSSGSAAIVAAGLCSAALGTDGG 312

Query: 221 GSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGADRY 280
           GS+  PS L  + G+K T G T   G +      + +GP+  ++ DA  V  AI+G+   
Sbjct: 313 GSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSA 372

Query: 281 DNSTIEAS-----KYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGHDETFYPQAYEKVLK 340
           D   ++ S     K +   G      +  +   R+G   ++++         +  E +LK
Sbjct: 373 DRYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSDISDKC-EDILK 432

Query: 341 TLKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASPI-------- 400
            L       V    +  L+   +  +   I++ +    SL  Y +    S +        
Sbjct: 433 LLSNNHGCKVVEIVVPELE---EMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSF 492

Query: 401 ---RSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEKAALARLAKLSKDGFERL 460
              RS S +     +  +   +EY   +F   +          A +     L K+G   +
Sbjct: 493 AIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDAL-KNGETNI 552

Query: 461 MIKNKLDAIAAPGSLISSVFAIGGFPGFSVPAGYDPQGVPYGITFGGLKGFEPRLIEIAY 512
            +   L       +L+       GFP  SVP GYD +G+P G+   G    E  ++ +A 
Sbjct: 553 QVTTDLMRFVLAANLL-------GFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAA 599