Pay0004080 (gene) Melon (Payzawat) v1

Overview
NamePay0004080
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr12: 25686039 .. 25687637 (+)
RNA-Seq ExpressionPay0004080
SyntenyPay0004080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAACACAATTTGTATTATTTGTGAAAAGACATTAATAAGAAGAATGGCAACAAATATGAGTGATGAACTCCCAGTTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAACGAAGGAATGGGTTTTCACAATGGAGAAAGTTCGAGAGGCTCTTGAAGAATATGGTTGTTTTGTAGCATTATACGACAGCGTTTCGGCAGAAGTAAGTTCGAAAATGTTTGATTCTTTAAAAGAATTGTTTGATGATGTTCCATTGGAGAGGAAGCTTCAGAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAATCCTCTAATGCCAATCCATGAAAGTATGGGTATCGAACACCCCATTCTCCCCACCAATATCAATTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACGACTCTTTCCGGTAATTATTCTTAATTTTGAAACGAAACAAAAACTCCATATTCATGATCTAATAATATGAGGATTATCCTAAACATTTTTGTTATTGTTGCAGTGAAAGTGTGACGACGTATGCAAAATTGGTATCGGAATTGGACGAAAGGGTGAAAAGGATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAAAAGTCATATGGAATCAACAAAATATCTTATGAGAATGATAAAATATAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCTTTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAATGAAGTCAATGGTTTGCAAATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTTATTGTCATGGCTGGAGATGCTTTCTTTGTAAGTTCATCTCATTTTATTAATTAAATTCTTCATCATCAAATTAAGATTAACGTTAAGATTAACATTAAGTTAATTAAGCCAAACTAATCATTAATCAATCGTTAATTGTTGGCAGGCATGGAGCAATGGGAGGATTTACTCACCACCACATAGAGTGATAATGAGTGGAAATAGAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATCGTTCAAATTCCTAAAGAATTGGTTGATGACAAACATCCCCAATTGTTTAACCCCTTTGATCATCAAGACTTTCTTCGTTTCTATAGCACCGAGAGAGGTCAAAATTCTCCTTGTGCCATCAAAGCTTATTGTGGAGTTGGAAATAGCCTCATTGATATGGATACTCGAAGTCCTCTTCTCTTGCATTGAGTAATAAATCCATCGTATCCATATCAATTTACTATTACGGATCATATCGAATGACTTTTTAAGTGTTTAAAATGTTGTATTTAAAACAAACACTTACAAAGTTATTCCAAATGAATAGATAACCTTAGTTTTATAATTTTAGAACCCACCTTGAATTTTGAGGCTATAGTCAGTTGGCTTGAAATGCCCAATTCATAGATGCTTACTTTTGTTTCTCTTTTGGTTTTTTTATGTCTATGATGTTGTAAAAATGTATAGCTCAAAGTAGAATCATTTCACAAGAAATGGTGTAATTTGATCTATTATTGAATTTGAGTCCACCTTTGAAGAATCTCAC

mRNA sequence

CAAAAACACAATTTGTATTATTTGTGAAAAGACATTAATAAGAAGAATGGCAACAAATATGAGTGATGAACTCCCAGTTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAACGAAGGAATGGGTTTTCACAATGGAGAAAGTTCGAGAGGCTCTTGAAGAATATGGTTGTTTTGTAGCATTATACGACAGCGTTTCGGCAGAAGTAAGTTCGAAAATGTTTGATTCTTTAAAAGAATTGTTTGATGATGTTCCATTGGAGAGGAAGCTTCAGAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAATCCTCTAATGCCAATCCATGAAAGTATGGGTATCGAACACCCCATTCTCCCCACCAATATCAATTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACGACTCTTTCCGTGAAAGTGTGACGACGTATGCAAAATTGGTATCGGAATTGGACGAAAGGGTGAAAAGGATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAAAAGTCATATGGAATCAACAAAATATCTTATGAGAATGATAAAATATAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCTTTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAATGAAGTCAATGGTTTGCAAATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTTATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGGAGGATTTACTCACCACCACATAGAGTGATAATGAGTGGAAATAGAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATCGTTCAAATTCCTAAAGAATTGGTTGATGACAAACATCCCCAATTGTTTAACCCCTTTGATCATCAAGACTTTCTTCGTTTCTATAGCACCGAGAGAGGTCAAAATTCTCCTTGTGCCATCAAAGCTTATTGTGGAGTTGGAAATAGCCTCATTGATATGGATACTCGAAGTCCTCTTCTCTTGCATTGAGTAATAAATCCATCGTATCCATATCAATTTACTATTACGGATCATATCGAATGACTTTTTAAGTGTTTAAAATGTTGTATTTAAAACAAACACTTACAAAGTTATTCCAAATGAATAGATAACCTTAGTTTTATAATTTTAGAACCCACCTTGAATTTTGAGGCTATAGTCAGTTGGCTTGAAATGCCCAATTCATAGATGCTTACTTTTGTTTCTCTTTTGGTTTTTTTATGTCTATGATGTTGTAAAAATGTATAGCTCAAAGTAGAATCATTTCACAAGAAATGGTGTAATTTGATCTATTATTGAATTTGAGTCCACCTTTGAAGAATCTCAC

Coding sequence (CDS)

ATGGCAACAAATATGAGTGATGAACTCCCAGTTGTGAAGTTTTGTTCGGAGAATTTGAAGAGTGGAACGAAGGAATGGGTTTTCACAATGGAGAAAGTTCGAGAGGCTCTTGAAGAATATGGTTGTTTTGTAGCATTATACGACAGCGTTTCGGCAGAAGTAAGTTCGAAAATGTTTGATTCTTTAAAAGAATTGTTTGATGATGTTCCATTGGAGAGGAAGCTTCAGAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAATCCTCTAATGCCAATCCATGAAAGTATGGGTATCGAACACCCCATTCTCCCCACCAATATCAATTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACGACTCTTTCCGTGAAAGTGTGACGACGTATGCAAAATTGGTATCGGAATTGGACGAAAGGGTGAAAAGGATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAAAAGTCATATGGAATCAACAAAATATCTTATGAGAATGATAAAATATAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCTTTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAATGAAGTCAATGGTTTGCAAATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTTATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGGAGGATTTACTCACCACCACATAGAGTGATAATGAGTGGAAATAGAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATCGTTCAAATTCCTAAAGAATTGGTTGATGACAAACATCCCCAATTGTTTAACCCCTTTGATCATCAAGACTTTCTTCGTTTCTATAGCACCGAGAGAGGTCAAAATTCTCCTTGTGCCATCAAAGCTTATTGTGGAGTTGGAAATAGCCTCATTGATATGGATACTCGAAGTCCTCTTCTCTTGCATTGA

Protein sequence

MATNMSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYSTERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH
Homology
BLAST of Pay0004080 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.9e-74
Identity = 135/319 (42.32%), Postives = 209/319 (65.52%), Query Frame = 0

Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++  +F++++
Sbjct: 9   SVSFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAME 68

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS KP+HGY   N    ++ES+GI+   +   +N FT  +WP  GN
Sbjct: 69  DLF-ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGN 128

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP-----K 183
            S  E++  +++ + ELD  V+RM+ ES+G+ N +  H+ ST YL R++KY  P      
Sbjct: 129 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 188

Query: 184 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 243
           ++E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  
Sbjct: 189 DEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLC 248

Query: 244 AWSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFNPFDHQDF 303
           A  NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++F PF++ DF
Sbjct: 249 ALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDF 308

Query: 304 LRFYSTERGQNSPCAIKAY 316
           L F+ TE G+ +  A+ A+
Sbjct: 309 LHFFQTEAGRIAQSALHAF 318

BLAST of Pay0004080 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.2e-73
Identity = 135/320 (42.19%), Postives = 208/320 (65.00%), Query Frame = 0

Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++  +F++++
Sbjct: 9   SVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAME 68

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN
Sbjct: 69  DLF-ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGN 128

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP------ 183
            S  E++  +++ + ELD  V+RM+ ES+G+ N +  H+ ST YL R++KY  P      
Sbjct: 129 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 188

Query: 184 KEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAF 243
            ++E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+ 
Sbjct: 189 DDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSL 248

Query: 244 FAWSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFNPFDHQD 303
            A  NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++F PF++ D
Sbjct: 249 CALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTD 308

Query: 304 FLRFYSTERGQNSPCAIKAY 316
           FL F+ TE G+ +  A+ A+
Sbjct: 309 FLHFFQTEAGRIAQSALHAF 319

BLAST of Pay0004080 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.0e-70
Identity = 131/309 (42.39%), Postives = 201/309 (65.05%), Query Frame = 0

Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++  +F++++
Sbjct: 2   SVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAME 61

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS K +HGY   N    ++ES+GI    +   +N FT  +WP  GN
Sbjct: 62  DLF-ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGN 121

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP----KE 183
            S  E++  +++ + ELD  V+RM+ ES+G+   +  H+ ST YL R++KY  P     +
Sbjct: 122 KSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDD 181

Query: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243
           +E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A
Sbjct: 182 EETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCA 241

Query: 244 WSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFNPFDHQDFL 303
             NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++F PF++ DFL
Sbjct: 242 LLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFL 301

Query: 304 RFYSTERGQ 307
            F+ TE G+
Sbjct: 302 HFFQTEAGR 301

BLAST of Pay0004080 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 8.8e-62
Identity = 125/307 (40.72%), Postives = 182/307 (59.28%), Query Frame = 0

Query: 9   LPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELFDD 68
           LPV+    +NLK GT  W     +VREALEEYGCF A+ D+VS E+   + +   EL  +
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELL-N 68

Query: 69  VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFRE 128
           +PLE K+ N ++  Y G+     +  ++E MG+        +  FTNLMWP  GN  F E
Sbjct: 69  LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCE 128

Query: 129 SVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAFP 188
           +V +Y K ++E+D  +K MVFES+G+     S  EST YL+  ++Y+ P +   +     
Sbjct: 129 TVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSL 188

Query: 189 HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSP 248
           H DKS LTI++QN+V GL+ +T+D +WI        +  + IV+ GD F AWSNGR++SP
Sbjct: 189 HKDKSILTIVNQNDVKGLEFETKDGEWI------LPTADNHIVLLGDCFMAWSNGRLHSP 248

Query: 249 PHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYSTERGQNS 308
            HRV +  N+ R S   FSF   I++ P ELVD++HP LFNPF+  + L +  T+ G  +
Sbjct: 249 LHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKA 304

Query: 309 PCAIKAY 316
            C +K Y
Sbjct: 309 VCDLKQY 304

BLAST of Pay0004080 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.0e-54
Identity = 129/434 (29.72%), Postives = 201/434 (46.31%), Query Frame = 0

Query: 8   ELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELFD 67
           +LP++    + L+ G+ +W      VR+ALE++GCF A YD VS E+   +  +++ELF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELF- 66

Query: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP-SRGNDSF 127
            +P+E K +NV  KPY GY   N L    ES+GI +  +  N+N FT  +WP   GN++ 
Sbjct: 67  ALPVETKQRNVCPKPYVGYLNHNNL---SESLGISNANILENVNEFTQQLWPDGDGNENI 126

Query: 128 RESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE------- 187
            +++  +A+ + E+D  V+RMV ES+G+   +  H++ST+Y MR++KY  P E       
Sbjct: 127 SKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 188 ------------------------------------------------------------ 247
                                                                       
Sbjct: 187 DDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDAE 246

Query: 248 -------------------------------------------------KEMNLGAFPHT 307
                                                            +E  LG   HT
Sbjct: 247 ANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCHT 306

Query: 308 DKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPH 322
           DK+  T+L Q+E+ GL++KT+D KWI+  P    S ++FIV+AGD+  A  NGRI +P H
Sbjct: 307 DKNLFTVLFQHEIEGLEVKTKDEKWIRVKP----SPNTFIVIAGDSLCALMNGRIRAPYH 366

BLAST of Pay0004080 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 7.7e-194
Identity = 330/334 (98.80%), Postives = 333/334 (99.70%), Query Frame = 0

Query: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFD 60
           MATNMSDELPVVKFCSENLKSGTKEWVFTM+KVREALE+YGCFVALYDSVSAEVSS MFD
Sbjct: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD 60

Query: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120
           SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS
Sbjct: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120

Query: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180
           RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK
Sbjct: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180

Query: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240
           EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW
Sbjct: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240

Query: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFY 300
           SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIP+ELVDDKHPQLFNPFDHQDFLRFY
Sbjct: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFY 300

Query: 301 STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 335
           STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH
Sbjct: 301 STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

BLAST of Pay0004080 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.7e-148
Identity = 260/328 (79.27%), Postives = 289/328 (88.11%), Query Frame = 0

Query: 8   ELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELFD 67
           +LP+VKF  ENL+S    WV T EKVREALEEYGCFVALYDSVS E+SS MFDSLKELF 
Sbjct: 5   QLPLVKFSEENLRS----WVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELF- 64

Query: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 127
           +VPLERKL+NVSEKPYHGYFGQNPLMPIHESMGIE P L +   SFTNLMWPS GN SF 
Sbjct: 65  EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFC 124

Query: 128 ESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAF 187
           E+V +YA+ ++ELDE VK+MVFESYGV   L+SHM+ST+YL+RMIKYRVPK+ EMN+GAF
Sbjct: 125 ENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAF 184

Query: 188 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHP-FSSSSTSSFIVMAGDAFFAWSNGRIY 247
           PHTDKSFLTILHQN+VNGLQIKT+ NKW+QYHP  SSSSTSSFIVMAGDAFFAWSNGRIY
Sbjct: 185 PHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGRIY 244

Query: 248 SPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYSTERGQ 307
           SPPH+VIMSG RERYSVGLFSFN+GIVQIPKELVD+ HPQ F PFDHQD+LRFYSTERGQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 308 NSPCAIKAYCGVGNSLIDMDTRSPLLLH 335
           NSPCAIKAYCG+GN L+  DTR  +LLH
Sbjct: 305 NSPCAIKAYCGIGNGLV--DTRGCILLH 325

BLAST of Pay0004080 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.4e-139
Identity = 244/329 (74.16%), Postives = 282/329 (85.71%), Query Frame = 0

Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSS-KMFDSLK 64
           M+ E+PVV+F S NL+SG ++W  T EKVREALEEYGCFVALYDSVS E SS KM +++K
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           E+F +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 61  EVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
             F E++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 121 PLFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSN
Sbjct: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSN 240

Query: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYST 304
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIPKELVDDKHPQ F  FDHQD+LRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPL 332
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 328

BLAST of Pay0004080 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 6.0e-138
Identity = 242/329 (73.56%), Postives = 281/329 (85.41%), Query Frame = 0

Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSS-KMFDSLK 64
           M+ ELPVV+F S NL+SG ++W  T EKVREALEEYGCFVAL DSVS E SS KM ++++
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           E+F +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 61  EVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
             F E++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 121 PLFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDAFFAWSN
Sbjct: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSN 240

Query: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYST 304
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIPKELVDDKHPQ F  FDHQD+LRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPL 332
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 328

BLAST of Pay0004080 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.1e-136
Identity = 240/329 (72.95%), Postives = 280/329 (85.11%), Query Frame = 0

Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSS-KMFDSLK 64
           M+ E+PVV+F S N++SG ++W  T EKVREALEEYGCFVAL DSVS E SS KM +++K
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           E+F +VPLERK+QNVS+KP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 61  EVF-EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
             F  ++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 121 PLFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSN
Sbjct: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSN 240

Query: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYST 304
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIPKELVDDKHPQ F  FDHQD+LRFYST
Sbjct: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300

Query: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPL 332
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 328

BLAST of Pay0004080 vs. NCBI nr
Match: TYJ99087.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 686.0 bits (1769), Expect = 1.6e-193
Identity = 330/334 (98.80%), Postives = 333/334 (99.70%), Query Frame = 0

Query: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFD 60
           MATNMSDELPVVKFCSENLKSGTKEWVFTM+KVREALE+YGCFVALYDSVSAEVSS MFD
Sbjct: 1   MATNMSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFD 60

Query: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120
           SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS
Sbjct: 61  SLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPS 120

Query: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180
           RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK
Sbjct: 121 RGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEK 180

Query: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240
           EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW
Sbjct: 181 EMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAW 240

Query: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFY 300
           SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIP+ELVDDKHPQLFNPFDHQDFLRFY
Sbjct: 241 SNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFY 300

Query: 301 STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 335
           STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH
Sbjct: 301 STERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

BLAST of Pay0004080 vs. NCBI nr
Match: XP_004151475.3 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570.1 hypothetical protein Csa_013560 [Cucumis sativus])

HSP 1 Score: 653.7 bits (1685), Expect = 8.7e-184
Identity = 315/335 (94.03%), Postives = 325/335 (97.01%), Query Frame = 0

Query: 1   MATNMSDE-LPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMF 60
           MATNMSDE LPVVKFCS+NLKSGT+EWV T +KVREALEEYGCFVALYD+VSAEVSS MF
Sbjct: 4   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 63

Query: 61  DSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 120
           DSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP
Sbjct: 64  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 123

Query: 121 SRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE 180
           SRGN SFRE+VT YA LVSELD+RVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPKE
Sbjct: 124 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 183

Query: 181 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 240
           KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA
Sbjct: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243

Query: 241 WSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRF 300
           WSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIPKELVDDKHPQLF PFDHQD+LRF
Sbjct: 244 WSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 303

Query: 301 YSTERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 335
           YSTERGQNSPCAIKAYCGVGNSLID DTRSPLLLH
Sbjct: 304 YSTERGQNSPCAIKAYCGVGNSLIDTDTRSPLLLH 338

BLAST of Pay0004080 vs. NCBI nr
Match: XP_031738134.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571.1 hypothetical protein Csa_014276 [Cucumis sativus])

HSP 1 Score: 653.3 bits (1684), Expect = 1.1e-183
Identity = 314/335 (93.73%), Postives = 325/335 (97.01%), Query Frame = 0

Query: 1   MATNMSDE-LPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMF 60
           MATNMSDE LPVVKFCS+NLKSGT+EWV T +KVREALEEYGCFVALYD+VSAEVSS MF
Sbjct: 4   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 63

Query: 61  DSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 120
           DSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP
Sbjct: 64  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 123

Query: 121 SRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE 180
           SRGN SFRE+VT YA LVSELD+RVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPKE
Sbjct: 124 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 183

Query: 181 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 240
           KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA
Sbjct: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243

Query: 241 WSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRF 300
           WSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIPKELVDDKHPQLF PFDHQD+LRF
Sbjct: 244 WSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 303

Query: 301 YSTERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 335
           YSTERGQNSPCAIKAYCGVGNSL+D DTRSPLLLH
Sbjct: 304 YSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338

BLAST of Pay0004080 vs. NCBI nr
Match: XP_038895877.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 607.4 bits (1565), Expect = 7.2e-170
Identity = 289/330 (87.58%), Postives = 308/330 (93.33%), Query Frame = 0

Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKE 64
           M  E+P+VKFCSENLKSG KEWV T EKVREALEEYGCFVALYD+VS +VSS MF SLK+
Sbjct: 1   MGGEIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQ 60

Query: 65  LFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGND 124
           LF DVPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPS GN 
Sbjct: 61  LF-DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNR 120

Query: 125 SFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNL 184
            F E+VT YAKLVSELDERVK MVFESYGVG  LK+HMESTKYLMRMIKYRVPKEKEMNL
Sbjct: 121 PFCENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNL 180

Query: 185 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGR 244
           GAFPHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGR
Sbjct: 181 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGR 240

Query: 245 IYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYSTER 304
           IYSPPHRV++S N+ERYSVGLFSFN+GI+QIPKELVDDKHPQ FNPFDHQD+LRFY+TER
Sbjct: 241 IYSPPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTER 300

Query: 305 GQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 335
           GQNSPCAIKAYCGVGNSL+D D+RS LLLH
Sbjct: 301 GQNSPCAIKAYCGVGNSLVDTDSRSTLLLH 329

BLAST of Pay0004080 vs. NCBI nr
Match: XP_038893580.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 604.4 bits (1557), Expect = 6.1e-169
Identity = 287/327 (87.77%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 8   ELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELFD 67
           ++P+VKFCSENLKSG KEWV T EKVREALEEYGCFVALYD+VS +VSS MF SLK+LF 
Sbjct: 5   QIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLF- 64

Query: 68  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 127
           DVPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPS GN  F 
Sbjct: 65  DVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFC 124

Query: 128 ESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAF 187
           E+VT YAKLVSELDERVK MVFESYGVG  LK+HMESTKYLMRMIKYRVPKEKEMNLGAF
Sbjct: 125 ENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAF 184

Query: 188 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 247
           PHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGRIYS 244

Query: 248 PPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYSTERGQN 307
           PPHRV++S N+ERYSVGLFSFN+GI+QIPKELVDDKHPQ FNPFDHQD+LRFY+TERGQN
Sbjct: 245 PPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQN 304

Query: 308 SPCAIKAYCGVGNSLIDMDTRSPLLLH 335
           SPCAIKAYCGVGNSL+D D+RS LLLH
Sbjct: 305 SPCAIKAYCGVGNSLVDTDSRSTLLLH 330

BLAST of Pay0004080 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 3.6e-87
Identity = 153/314 (48.73%), Postives = 216/314 (68.79%), Query Frame = 0

Query: 5   MSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVA-LYDSVSAEVSSKMFDSLK 64
           MS ++P + F  E+LK GTK W  T E +R+ALEEYGCF+  L D    ++  ++F SL 
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLV 60

Query: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124
           +LF D+P + K++N  +KP +GY GQ P +P+HES+GI++        SFT LMWP +GN
Sbjct: 61  DLF-DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGN 120

Query: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184
           + F E +  YA+  +ELD+ V RMVF+SY V      ++EST YL+R++K R P  +   
Sbjct: 121 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 180

Query: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNG 244
           LG   HTDKSF TILHQ++VNGL+++TR+ + I     + SS S F+V+AGDA  AWSN 
Sbjct: 181 LGFVTHTDKSFTTILHQDQVNGLEMETREGERIN---INLSSPSLFMVVAGDALMAWSND 240

Query: 245 RIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYSTE 304
           R++SP H+V++SG  +RYS+G+F+FNNG +Q+P+EL+D +HP ++ PFDH   LRFY T+
Sbjct: 241 RVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTD 300

Query: 305 RGQNSPCAIKAYCG 318
            G  S C IKAYCG
Sbjct: 301 TGYKSECPIKAYCG 309

BLAST of Pay0004080 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 4.4e-85
Identity = 146/312 (46.79%), Postives = 217/312 (69.55%), Query Frame = 0

Query: 9   LPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELFDD 68
           LPV+ F ++NLK G  EW  T   V++AL++YG F A +D +  E+   +F +L+ELF D
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELF-D 71

Query: 69  VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFRE 128
           +PL+ KL+NVS+KP+HGY GQ P++P++ESMGI+   +   +++FT  +WP +GN SF  
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 131

Query: 129 SVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGAFP 188
           ++ +++K +SELD  ++RM+ ES+G+   +  H+ ST YL+R++KY+ P  +E  +G   
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 189 HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSP 248
           HTDK+ +TIL+QN V GL+++T+D  WI+  P    +  SF VM GD+ +A  NGR++SP
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP----TQDSFTVMIGDSLYALLNGRLHSP 251

Query: 249 PHRVIMSGNRERYSVGLFSFNNG--IVQIPKELVDDKHPQLFNPFDHQDFLRFYSTERGQ 308
            HRV+M+G   RYS+GLFS      IV  P ELVD++HP+LF PFDH +FL+FY TE GQ
Sbjct: 252 YHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQ 311

Query: 309 NSPCAIKAYCGV 319
            S  A+K YCG+
Sbjct: 312 RSQSALKTYCGI 317

BLAST of Pay0004080 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.7e-81
Identity = 145/313 (46.33%), Postives = 211/313 (67.41%), Query Frame = 0

Query: 8   ELPVVKFCS-ENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELF 67
           ++P++   S + LK  T  W     +  EALEEYGCF+A+YD V+ ++   +F + +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  DDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSF 127
            D+P E K +NV+EKPYHGY GQ P++P+HE +G+++         FT+LMWP +GND F
Sbjct: 69  -DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF 128

Query: 128 RESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNLGA 187
             +V T++  V+ELD  V RM+FE+YGV    +SH+ S  YL++ +KY  P E  +++ A
Sbjct: 129 CNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPE-SISMPA 188

Query: 188 FP-HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRI 247
           FP HTDK+FL+ILHQN+VNGL++K++D +WI           S++VMAGD    WSN RI
Sbjct: 189 FPQHTDKTFLSILHQNDVNGLEVKSKDGEWISL----QLPPKSYVVMAGDISMGWSNDRI 248

Query: 248 YSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYSTERG 307
            S  HRV M G++ RY++GLFSF   +V IP+ELVDDKHP ++ PFD+   + FY+T+ G
Sbjct: 249 RSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEG 308

Query: 308 QNSPCAIKAYCGV 319
           + +   +KAYCG+
Sbjct: 309 REANSTLKAYCGI 314

BLAST of Pay0004080 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 277.3 bits (708), Expect = 1.6e-74
Identity = 135/320 (42.19%), Postives = 208/320 (65.00%), Query Frame = 0

Query: 4   NMSDELPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLK 63
           ++S +LPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++  +F++++
Sbjct: 9   SVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAME 68

Query: 64  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 123
           +LF ++P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN
Sbjct: 69  DLF-ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGN 128

Query: 124 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVP------ 183
            S  E++  +++ + ELD  V+RM+ ES+G+ N +  H+ ST YL R++KY  P      
Sbjct: 129 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDD 188

Query: 184 KEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAF 243
            ++E  LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+ 
Sbjct: 189 DDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSL 248

Query: 244 FAWSNGRIYSPPHRVIMSGNRERYSVGLFSF--NNGIVQIPKELVDDKHPQLFNPFDHQD 303
            A  NGR++SP HRVIM+G + RYS GLFS      I+  P+ELVD +HP++F PF++ D
Sbjct: 249 CALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTD 308

Query: 304 FLRFYSTERGQNSPCAIKAY 316
           FL F+ TE G+ +  A+ A+
Sbjct: 309 FLHFFQTEAGRIAQSALHAF 319

BLAST of Pay0004080 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 2.5e-67
Identity = 130/316 (41.14%), Postives = 195/316 (61.71%), Query Frame = 0

Query: 9   LPVVKFCSENLKSGTKEWVFTMEKVREALEEYGCFVALYDSVSAEVSSKMFDSLKELFDD 68
           LP++ F S+ L  GT  W+ T + VR A+E  G FVA +  VS+++   +   +KE++  
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMY-Y 68

Query: 69  VPLERKLQNVSEKPYHGYFGQ-NPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNDSFR 128
           +P + K++N + K  HGY         IHES+GI++         F+ L+WP +GND F 
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFC 128

Query: 129 ESVTTYAKLVSELDERVKRMVFESYGVGNALK--SHMESTKYLMRMIKYRVPKEKEMNLG 188
           ++   YA  ++ELD+ V RM++ESYG+       SH EST+YL+RM+ YR  +  E N G
Sbjct: 129 QTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTG 188

Query: 189 AFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRI 248
              HTDKSF++ILHQN V GLQ+KT   +W+ ++P    S + F+V++G    AWSN RI
Sbjct: 189 FVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP----SPTRFVVLSGMGLTAWSNDRI 248

Query: 249 YSPPHRVIMSGNRERYSVGLFSFNNGIVQIPKELVDDKHPQLFNPFDHQDFLRFYST--- 308
            +  H+V+MS +  RYS+G FSF+ G ++ P+ELVDD+HP  +NPF+H   LRFY +   
Sbjct: 249 KACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYIN 308

Query: 309 ERGQNSPCAIKAYCGV 319
              ++S   ++ YCGV
Sbjct: 309 SLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q945B65.9e-7442.32Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA32.2e-7342.19Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q944Z51.0e-7042.39Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ368.8e-6240.72Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q945B53.0e-5429.722-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
A0A5D3BIZ77.7e-19498.80Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O... [more]
A0A6J1C7123.7e-14879.27probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara... [more]
A0A6J1EAT72.4e-13974.16probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1E7C86.0e-13873.56probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1KFF71.1e-13672.95probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
TYJ99087.11.6e-19398.80putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa][more]
XP_004151475.38.7e-18494.03probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570... [more]
XP_031738134.11.1e-18393.73probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571... [more]
XP_038895877.17.2e-17087.58probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
XP_038893580.16.1e-16987.77probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G52790.13.6e-8748.732-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.14.4e-8546.792-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.11.7e-8146.332-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G03070.11.6e-7442.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.12.5e-6741.142-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 174..268
e-value: 5.3E-18
score: 65.4
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..299
e-value: 1.6E-55
score: 191.0
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 25..88
e-value: 1.7E-7
score: 32.0
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 6..317
NoneNo IPR availablePANTHERPTHR47990:SF172-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKEcoord: 6..317
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 7..305
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 165..271
score: 10.976438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0004080.1Pay0004080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding