Pay0003489 (gene) Melon (Payzawat) v1

Overview
NamePay0003489
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionPectinesterase
Locationchr06: 8686322 .. 8687277 (+)
RNA-Seq ExpressionPay0003489
SyntenyPay0003489
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCACGACGACCGGGGCTCCGATCTGTCCCCGTCGCATCTGCCTTCAACTCTCCGCCCACCACCACTCAATCGGAGCACCACACGCCTTCTCCTTATTTCCTTCCTCTCTCTCCTTCTCATCGTCGCCGCCGCCGTCTCAGCTACCTTCCTCATCGGACTACGAACCGCCTCCCCTGCTCGATCCGACCCAAATCTCCCTCGTAAACCCACTCAGGCAATTTCCAAAGCTTGTAGCCGAACTCGCTTCCCCACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGGCTCTCTCAACGCCAACGAACAAGATCTCGTTCACATTTCCTTCAATATCACTCTCCAACATCTCAACAAAGCCCTCTATTCATCCTCTGAGATTTCCTCTCTTCAAATGGATCTCCGTGTTCGTTCCGCTTACGATGCTTGTCTCGAACTCCTCGACGACTCCATCGACGCTGTTTCTCGTTCCTTGCAATCCGTCGCACCGTCTTCCTCATCCTCCACTCCGCAGCGGCTCGGCTCATCTGAAGATATCATTACCTGGCTTAGCGCTGCCCTAACTAATCAAGACACATGTACGGATGGATTCTCTGAACTCTCCGGTACTGTGAAAAACCAAATGGCAGATAAACTTCACAATCTGTCTGAACTTGTTAGTAATTGCTTGGCGCTGTTTTCCGGAAGCGAAACGAGCGATTTCGCCGGAGTGCCGATTCAGAACAAACGACGGTTGATGAAGGAGGAAGGAGAGAATGAGGATAGTTCCGGTAACTTCCCGAGATGGATGAGGGGGAGAGAGAGGAGGTTACTGACGTTGCCGGTGGGAGTGTTACAAGCGGATATTGTGGTTTCTCAGAACGGTAACGGAACGGTGAAGACGATCGCGGAAGCGATAAAAAAGGCGCCGCAGTACAGTAATAGAAGGACGATCATTTACGTGATGGCAGGAAGGTAA

mRNA sequence

CTCACGACGACCGGGGCTCCGATCTGTCCCCGTCGCATCTGCCTTCAACTCTCCGCCCACCACCACTCAATCGGAGCACCACACGCCTTCTCCTTATTTCCTTCCTCTCTCTCCTTCTCATCGTCGCCGCCGCCGTCTCAGCTACCTTCCTCATCGGACTACGAACCGCCTCCCCTGCTCGATCCGACCCAAATCTCCCTCGTAAACCCACTCAGGCAATTTCCAAAGCTTGTAGCCGAACTCGCTTCCCCACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGGCTCTCTCAACGCCAACGAACAAGATCTCGTTCACATTTCCTTCAATATCACTCTCCAACATCTCAACAAAGCCCTCTATTCATCCTCTGAGATTTCCTCTCTTCAAATGGATCTCCGTGTTCGTTCCGCTTACGATGCTTGTCTCGAACTCCTCGACGACTCCATCGACGCTGTTTCTCGTTCCTTGCAATCCGTCGCACCGTCTTCCTCATCCTCCACTCCGCAGCGGCTCGGCTCATCTGAAGATATCATTACCTGGCTTAGCGCTGCCCTAACTAATCAAGACACATGTACGGATGGATTCTCTGAACTCTCCGGTACTGTGAAAAACCAAATGGCAGATAAACTTCACAATCTGTCTGAACTTGTTAGTAATTGCTTGGCGCTGTTTTCCGGAAGCGAAACGAGCGATTTCGCCGGAGTGCCGATTCAGAACAAACGACGGTTGATGAAGGAGGAAGGAGAGAATGAGGATAGTTCCGGTAACTTCCCGAGATGGATGAGGGGGAGAGAGAGGAGGTTACTGACGTTGCCGGTGGGAGTGTTACAAGCGGATATTGTGGTTTCTCAGAACGGTAACGGAACGGTGAAGACGATCGCGGAAGCGATAAAAAAGGCGCCGCAGTACAGTAATAGAAGGACGATCATTTACGTGATGGCAGGAAGGTAA

Coding sequence (CDS)

CTCACGACGACCGGGGCTCCGATCTGTCCCCGTCGCATCTGCCTTCAACTCTCCGCCCACCACCACTCAATCGGAGCACCACACGCCTTCTCCTTATTTCCTTCCTCTCTCTCCTTCTCATCGTCGCCGCCGCCGTCTCAGCTACCTTCCTCATCGGACTACGAACCGCCTCCCCTGCTCGATCCGACCCAAATCTCCCTCGTAAACCCACTCAGGCAATTTCCAAAGCTTGTAGCCGAACTCGCTTCCCCACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGGCTCTCTCAACGCCAACGAACAAGATCTCGTTCACATTTCCTTCAATATCACTCTCCAACATCTCAACAAAGCCCTCTATTCATCCTCTGAGATTTCCTCTCTTCAAATGGATCTCCGTGTTCGTTCCGCTTACGATGCTTGTCTCGAACTCCTCGACGACTCCATCGACGCTGTTTCTCGTTCCTTGCAATCCGTCGCACCGTCTTCCTCATCCTCCACTCCGCAGCGGCTCGGCTCATCTGAAGATATCATTACCTGGCTTAGCGCTGCCCTAACTAATCAAGACACATGTACGGATGGATTCTCTGAACTCTCCGGTACTGTGAAAAACCAAATGGCAGATAAACTTCACAATCTGTCTGAACTTGTTAGTAATTGCTTGGCGCTGTTTTCCGGAAGCGAAACGAGCGATTTCGCCGGAGTGCCGATTCAGAACAAACGACGGTTGATGAAGGAGGAAGGAGAGAATGAGGATAGTTCCGGTAACTTCCCGAGATGGATGAGGGGGAGAGAGAGGAGGTTACTGACGTTGCCGGTGGGAGTGTTACAAGCGGATATTGTGGTTTCTCAGAACGGTAACGGAACGGTGAAGACGATCGCGGAAGCGATAAAAAAGGCGCCGCAGTACAGTAATAGAAGGACGATCATTTACGTGATGGCAGGAAGGTAA

Protein sequence

HDDRGSDLSPSHLPSTLRPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR
Homology
BLAST of Pay0003489 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 7.0e-77
Identity = 169/304 (55.59%), Postives = 218/304 (71.71%), Query Frame = 0

Query: 18  RPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPA-RSDPNLPRKPTQAISKA 77
           +P   NR + + L++S  S++L ++  ++A    G+R+     +S P L RKP+QAISKA
Sbjct: 33  QPENNNRRSKKKLVVS--SIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKA 92

Query: 78  CSRTRFPTLCVNSLLDFPGSLNA-NEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRV 137
           C  TRFP LCV+SL+DFPGSL A + +DL+H++ N+TL H + ALYSS+ +S + M  R 
Sbjct: 93  CELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRA 152

Query: 138 RSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDG 197
           RSAYD+C+ELLDDS+DA+SR+L SV   SSS+ PQ      D+ TWLSAALTN DTCT+G
Sbjct: 153 RSAYDSCVELLDDSVDALSRALSSVV--SSSAKPQ------DVTTWLSAALTNHDTCTEG 212

Query: 198 FSEL-SGTVKNQMADKLHNLSELVSNCLALFSGS-ETSDFAGVPIQNKRRLMKEEGENED 257
           F  +  G VK+ M   L NLSELVSNCLA+FS S +  DFAGVPIQN+R L  EE E + 
Sbjct: 213 FDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEK- 272

Query: 258 SSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYV 317
               FPRWMR +ER +L +PV  +QADI+VS++GNGT KTI+EAIKKAPQ S RR IIYV
Sbjct: 273 ----FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYV 321

BLAST of Pay0003489 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-73
Identity = 152/307 (49.51%), Postives = 210/307 (68.40%), Query Frame = 0

Query: 14  PSTLRPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAI 73
           P+T  P    ++ T+L+L    +L ++V   V      G+R     +++P L RKPTQAI
Sbjct: 19  PATSLPELQKKTKTKLIL---FTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAI 78

Query: 74  SKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDL 133
           S+ CS++ +P LC+++LLDFPGSL A+E +L+HISFN TLQ  +KALY+SS I+  QM  
Sbjct: 79  SRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPP 138

Query: 134 RVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCT 193
           RVRSAYD+CLELLDDS+DA++R+L SV   S         S  D++TWLS+A+TN DTCT
Sbjct: 139 RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE------SHSDVMTWLSSAMTNHDTCT 198

Query: 194 DGFSEL---SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGE 253
           DGF E+    G VK+Q+   + +LSE+VSNCLA+F+G +  D +GVP+ N R+L+  E  
Sbjct: 199 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAG-KVKDLSGVPVVNNRKLLGTEET 258

Query: 254 NEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTI 313
            E      P W++  +R LL  P   +QADI VS++G+GT KTIAEAIKKAP++S+RR +
Sbjct: 259 EE-----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFV 310

Query: 314 IYVMAGR 318
           IYV AGR
Sbjct: 319 IYVKAGR 310

BLAST of Pay0003489 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 86/294 (29.25%), Postives = 142/294 (48.30%), Query Frame = 0

Query: 28  RLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCV 87
           R  ++  +S +L+++  V+ T  + L                 +A+   C+ T +   C 
Sbjct: 13  RRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCE 72

Query: 88  NSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLD 147
           ++L+   G    +  +LV  +FN+T++ +  A   S  I  LQ D R R A D C EL+D
Sbjct: 73  DTLIK-NGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELMD 132

Query: 148 DSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQM 207
            ++D +S S + +         + L    ++  WLSAA+++++TC +GF    G     M
Sbjct: 133 YALDELSNSFEELGKFEFHLLDEAL---INLRIWLSAAISHEETCLEGFQGTQGNAGETM 192

Query: 208 ADKLHNLSELVSNCLALFSGSETSDFAG---VPIQNKRRLMKEEGENEDSSGNFPRWMRG 267
              L    EL  N LA+   SE S+F G   +P  N RRL+ E          FP W+  
Sbjct: 193 KKALKTAIELTHNGLAII--SEMSNFVGQMQIPGLNSRRLLAE---------GFPSWVDQ 252

Query: 268 RERRLLTLPVGV--LQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAG 317
           R R+LL        ++ DIVV+Q+G+G  KTI EA++  P+  N   ++++ AG
Sbjct: 253 RGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAG 291

BLAST of Pay0003489 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.2e-23
Identity = 86/292 (29.45%), Postives = 143/292 (48.97%), Query Frame = 0

Query: 28  RLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCV 87
           R ++IS  S+LLI +  V+ T  + +  +  A  D  +     +AI   C+ T +   C 
Sbjct: 15  RYVIISISSVLLI-SMVVAVTIGVSVNKSDNA-GDEEITTS-VKAIKDVCAPTDYKETCE 74

Query: 88  NSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLD 147
           ++L        ++  +LV  +FN T++ ++     S  +  LQ D R + A D C EL+D
Sbjct: 75  DTLRK-DAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELMD 134

Query: 148 DSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQM 207
            +I  +S+S + +         + L     +  WLSA ++++ TC DGF    G     +
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEAL---VKLRIWLSATISHEQTCLDGFQGTQGNAGETI 194

Query: 208 ADKLHNLSELVSNCLALFSGSETSDFAG---VPIQNKRRLMKEEGENEDSSGNFPRWMRG 267
              L    +L  N LA+   +E S++ G   +P  N RRL+ +E         FP WM  
Sbjct: 195 KKALKTAVQLTHNGLAMV--TEMSNYLGQMQIPEMNSRRLLSQE---------FPSWMDA 254

Query: 268 RERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAG 317
           R RRLL  P+  ++ DIVV+Q+G+G  KTI EA+   P+  N   ++++  G
Sbjct: 255 RARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEG 288

BLAST of Pay0003489 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 3.4e-23
Identity = 85/312 (27.24%), Postives = 155/312 (49.68%), Query Frame = 0

Query: 24  RSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFP 83
           +   +L+L+S    LL VAA    +       AS A     L       +  +CS TR+P
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGIS-----AGASKANEKRTLSPSSHAVLRSSCSSTRYP 74

Query: 84  TLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDL--RVRSAYDA 143
            LC+++++   G    +++D++  S N+T+  +    ++  ++   +  L  R ++A   
Sbjct: 75  ELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHD 134

Query: 144 CLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFS--EL 203
           CLE +D+++D +  +++ +    +  T +    + D+ T +S+A+TNQ+TC DGFS  + 
Sbjct: 135 CLETIDETLDELHETVEDLHLYPTKKTLRE--HAGDLKTLISSAITNQETCLDGFSHDDA 194

Query: 204 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAG----VPIQNKRRLMKEE------- 263
              V+  +     ++  + SN LA+      +D A       I +  R +KEE       
Sbjct: 195 DKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVA 254

Query: 264 ----GENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQY 317
               G  E  S  +P W+   +RRL  L    ++AD  V+ +G+GT KT+A A+  AP+ 
Sbjct: 255 VDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFKTVAAAVAAAPEN 314

BLAST of Pay0003489 vs. ExPASy TrEMBL
Match: A0A5A7U3N7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001450 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.8e-155
Identity = 296/298 (99.33%), Postives = 296/298 (99.33%), Query Frame = 0

Query: 20  PPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 79
           P  NRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR
Sbjct: 31  PTPNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 90

Query: 80  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 139
           TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY
Sbjct: 91  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 150

Query: 140 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 199
           DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL
Sbjct: 151 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 210

Query: 200 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 259
           SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP
Sbjct: 211 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 270

Query: 260 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 318
           RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR
Sbjct: 271 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 328

BLAST of Pay0003489 vs. ExPASy TrEMBL
Match: A0A1S3CP11 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503123 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.8e-155
Identity = 296/298 (99.33%), Postives = 296/298 (99.33%), Query Frame = 0

Query: 20  PPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 79
           P  NRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR
Sbjct: 31  PTPNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 90

Query: 80  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 139
           TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY
Sbjct: 91  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 150

Query: 140 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 199
           DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL
Sbjct: 151 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 210

Query: 200 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 259
           SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP
Sbjct: 211 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 270

Query: 260 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 318
           RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR
Sbjct: 271 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 328

BLAST of Pay0003489 vs. ExPASy TrEMBL
Match: A0A5D3E0Z2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G003700 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.5e-154
Identity = 295/298 (98.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 20  PPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 79
           P  NRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR
Sbjct: 31  PTPNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 90

Query: 80  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 139
           TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY
Sbjct: 91  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 150

Query: 140 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 199
           DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL
Sbjct: 151 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 210

Query: 200 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 259
           SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP
Sbjct: 211 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 270

Query: 260 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 318
           RWMR RERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR
Sbjct: 271 RWMRERERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 328

BLAST of Pay0003489 vs. ExPASy TrEMBL
Match: A0A0A0L6S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.1e-152
Identity = 294/312 (94.23%), Postives = 298/312 (95.51%), Query Frame = 0

Query: 6   SDLSPSHLPSTLRPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNL 65
           S  SP    S+  P P NR TTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNL
Sbjct: 18  SPASPPFQLSSHHPTP-NRRTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNL 77

Query: 66  PRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSE 125
           PRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN+TLQHLNKALYSSSE
Sbjct: 78  PRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNVTLQHLNKALYSSSE 137

Query: 126 ISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAA 185
           ISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAA
Sbjct: 138 ISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAA 197

Query: 186 LTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLM 245
           LTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLM
Sbjct: 198 LTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLM 257

Query: 246 KEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYS 305
           +EEGENEDSSG FPRWM  RERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYS
Sbjct: 258 EEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYS 317

Query: 306 NRRTIIYVMAGR 318
           +RRTIIYVMAGR
Sbjct: 318 SRRTIIYVMAGR 328

BLAST of Pay0003489 vs. ExPASy TrEMBL
Match: A0A6J1E487 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.8e-137
Identity = 267/309 (86.41%), Postives = 283/309 (91.59%), Query Frame = 0

Query: 9   SPSHLPSTLRPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRK 68
           +PSHL      P   RS  RLLLIS LSL+LIVAAAVSATFL+GLRTAS  RSDPNLPRK
Sbjct: 18  APSHLSHHAASPNPRRS--RLLLISLLSLILIVAAAVSATFLVGLRTASSVRSDPNLPRK 77

Query: 69  PTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISS 128
           PTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN+TLQHLNKALY SS ISS
Sbjct: 78  PTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTLQHLNKALYFSSGISS 137

Query: 129 LQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTN 188
           LQMDLRVRSAYDACLELLDDS+DA++RSLQSVAPSSS+  PQRLGSS+D+ITWLSAALTN
Sbjct: 138 LQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLGSSDDVITWLSAALTN 197

Query: 189 QDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEE 248
           QDTCTDGFS+LSG+VK+QMADKLHNLSELVSNCLALFSGS TSDF+GVPIQNKRRLM   
Sbjct: 198 QDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDFSGVPIQNKRRLM-NA 257

Query: 249 GENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRR 308
           GENED+SGNFP WM  RERRLLTLPVG LQADIVVSQNG+GTVKTIAEAIKKAPQYSNRR
Sbjct: 258 GENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTIAEAIKKAPQYSNRR 317

Query: 309 TIIYVMAGR 318
           TIIYVMAGR
Sbjct: 318 TIIYVMAGR 323

BLAST of Pay0003489 vs. NCBI nr
Match: KAA0050503.1 (pectin methylesterase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 558.5 bits (1438), Expect = 3.6e-155
Identity = 296/298 (99.33%), Postives = 296/298 (99.33%), Query Frame = 0

Query: 20  PPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 79
           P  NRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR
Sbjct: 31  PTPNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 90

Query: 80  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 139
           TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY
Sbjct: 91  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 150

Query: 140 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 199
           DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL
Sbjct: 151 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 210

Query: 200 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 259
           SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP
Sbjct: 211 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 270

Query: 260 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 318
           RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR
Sbjct: 271 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 328

BLAST of Pay0003489 vs. NCBI nr
Match: XP_008465502.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 34 [Cucumis melo])

HSP 1 Score: 558.5 bits (1438), Expect = 3.6e-155
Identity = 296/298 (99.33%), Postives = 296/298 (99.33%), Query Frame = 0

Query: 20  PPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 79
           P  NRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR
Sbjct: 31  PTPNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 90

Query: 80  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 139
           TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY
Sbjct: 91  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 150

Query: 140 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 199
           DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL
Sbjct: 151 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 210

Query: 200 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 259
           SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP
Sbjct: 211 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 270

Query: 260 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 318
           RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR
Sbjct: 271 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 328

BLAST of Pay0003489 vs. NCBI nr
Match: TYK29180.1 (putative pectinesterase/pectinesterase inhibitor 34 [Cucumis melo var. makuwa])

HSP 1 Score: 555.4 bits (1430), Expect = 3.1e-154
Identity = 295/298 (98.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 20  PPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 79
           P  NRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR
Sbjct: 31  PTPNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 90

Query: 80  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 139
           TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY
Sbjct: 91  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 150

Query: 140 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 199
           DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL
Sbjct: 151 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 210

Query: 200 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 259
           SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP
Sbjct: 211 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 270

Query: 260 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 318
           RWMR RERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR
Sbjct: 271 RWMRERERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 328

BLAST of Pay0003489 vs. NCBI nr
Match: XP_004146219.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucumis sativus] >KGN57675.1 hypothetical protein Csa_009694 [Cucumis sativus])

HSP 1 Score: 547.4 bits (1409), Expect = 8.4e-152
Identity = 294/312 (94.23%), Postives = 298/312 (95.51%), Query Frame = 0

Query: 6   SDLSPSHLPSTLRPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNL 65
           S  SP    S+  P P NR TTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNL
Sbjct: 18  SPASPPFQLSSHHPTP-NRRTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNL 77

Query: 66  PRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSE 125
           PRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN+TLQHLNKALYSSSE
Sbjct: 78  PRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNVTLQHLNKALYSSSE 137

Query: 126 ISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAA 185
           ISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAA
Sbjct: 138 ISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAA 197

Query: 186 LTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLM 245
           LTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLM
Sbjct: 198 LTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLM 257

Query: 246 KEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYS 305
           +EEGENEDSSG FPRWM  RERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYS
Sbjct: 258 EEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYS 317

Query: 306 NRRTIIYVMAGR 318
           +RRTIIYVMAGR
Sbjct: 318 SRRTIIYVMAGR 328

BLAST of Pay0003489 vs. NCBI nr
Match: XP_038881267.1 (probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida])

HSP 1 Score: 508.8 bits (1309), Expect = 3.3e-140
Identity = 270/298 (90.60%), Postives = 282/298 (94.63%), Query Frame = 0

Query: 20  PPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSR 79
           P  +RS  +L LIS LSLLLIVAAAVSATFLIG+RTASPARSD NLPRKPTQAISKACSR
Sbjct: 29  PTPSRSRAQLFLISLLSLLLIVAAAVSATFLIGIRTASPARSDRNLPRKPTQAISKACSR 88

Query: 80  TRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAY 139
           TRFP+LCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALY SSEISSLQMDLRVRSAY
Sbjct: 89  TRFPSLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYLSSEISSLQMDLRVRSAY 148

Query: 140 DACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSEL 199
           DACLELLDDS+DA++RSLQSVAPSSSS TPQRLGSSED+ITWLSAALTNQDTCTDGFSEL
Sbjct: 149 DACLELLDDSVDALTRSLQSVAPSSSSGTPQRLGSSEDVITWLSAALTNQDTCTDGFSEL 208

Query: 200 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGENEDSSGNFP 259
           SGTVKNQMADKLHNLSELVSNCLALFSGSETSDF+GVPIQNKRRLM   G+NED+SGNFP
Sbjct: 209 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFSGVPIQNKRRLM-NAGDNEDTSGNFP 268

Query: 260 RWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAGR 318
            WM  RERRLLTLPVG LQADIVVSQNGNGTVKTIAEAIKKAPQYS+RRTIIYVMAGR
Sbjct: 269 GWMSRRERRLLTLPVGALQADIVVSQNGNGTVKTIAEAIKKAPQYSSRRTIIYVMAGR 325

BLAST of Pay0003489 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 288.9 bits (738), Expect = 5.0e-78
Identity = 169/304 (55.59%), Postives = 218/304 (71.71%), Query Frame = 0

Query: 18  RPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPA-RSDPNLPRKPTQAISKA 77
           +P   NR + + L++S  S++L ++  ++A    G+R+     +S P L RKP+QAISKA
Sbjct: 33  QPENNNRRSKKKLVVS--SIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKA 92

Query: 78  CSRTRFPTLCVNSLLDFPGSLNA-NEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRV 137
           C  TRFP LCV+SL+DFPGSL A + +DL+H++ N+TL H + ALYSS+ +S + M  R 
Sbjct: 93  CELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRA 152

Query: 138 RSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDG 197
           RSAYD+C+ELLDDS+DA+SR+L SV   SSS+ PQ      D+ TWLSAALTN DTCT+G
Sbjct: 153 RSAYDSCVELLDDSVDALSRALSSVV--SSSAKPQ------DVTTWLSAALTNHDTCTEG 212

Query: 198 FSEL-SGTVKNQMADKLHNLSELVSNCLALFSGS-ETSDFAGVPIQNKRRLMKEEGENED 257
           F  +  G VK+ M   L NLSELVSNCLA+FS S +  DFAGVPIQN+R L  EE E + 
Sbjct: 213 FDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEK- 272

Query: 258 SSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYV 317
               FPRWMR +ER +L +PV  +QADI+VS++GNGT KTI+EAIKKAPQ S RR IIYV
Sbjct: 273 ----FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYV 321

BLAST of Pay0003489 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 278.1 bits (710), Expect = 8.8e-75
Identity = 152/307 (49.51%), Postives = 210/307 (68.40%), Query Frame = 0

Query: 14  PSTLRPPPLNRSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAI 73
           P+T  P    ++ T+L+L    +L ++V   V      G+R     +++P L RKPTQAI
Sbjct: 19  PATSLPELQKKTKTKLIL---FTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAI 78

Query: 74  SKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDL 133
           S+ CS++ +P LC+++LLDFPGSL A+E +L+HISFN TLQ  +KALY+SS I+  QM  
Sbjct: 79  SRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPP 138

Query: 134 RVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCT 193
           RVRSAYD+CLELLDDS+DA++R+L SV   S         S  D++TWLS+A+TN DTCT
Sbjct: 139 RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE------SHSDVMTWLSSAMTNHDTCT 198

Query: 194 DGFSEL---SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMKEEGE 253
           DGF E+    G VK+Q+   + +LSE+VSNCLA+F+G +  D +GVP+ N R+L+  E  
Sbjct: 199 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAG-KVKDLSGVPVVNNRKLLGTEET 258

Query: 254 NEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTI 313
            E      P W++  +R LL  P   +QADI VS++G+GT KTIAEAIKKAP++S+RR +
Sbjct: 259 EE-----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFV 310

Query: 314 IYVMAGR 318
           IYV AGR
Sbjct: 319 IYVKAGR 310

BLAST of Pay0003489 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 115.2 bits (287), Expect = 9.9e-26
Identity = 86/294 (29.25%), Postives = 142/294 (48.30%), Query Frame = 0

Query: 28  RLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCV 87
           R  ++  +S +L+++  V+ T  + L                 +A+   C+ T +   C 
Sbjct: 13  RRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCE 72

Query: 88  NSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLD 147
           ++L+   G    +  +LV  +FN+T++ +  A   S  I  LQ D R R A D C EL+D
Sbjct: 73  DTLIK-NGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELMD 132

Query: 148 DSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQM 207
            ++D +S S + +         + L    ++  WLSAA+++++TC +GF    G     M
Sbjct: 133 YALDELSNSFEELGKFEFHLLDEAL---INLRIWLSAAISHEETCLEGFQGTQGNAGETM 192

Query: 208 ADKLHNLSELVSNCLALFSGSETSDFAG---VPIQNKRRLMKEEGENEDSSGNFPRWMRG 267
              L    EL  N LA+   SE S+F G   +P  N RRL+ E          FP W+  
Sbjct: 193 KKALKTAIELTHNGLAII--SEMSNFVGQMQIPGLNSRRLLAE---------GFPSWVDQ 252

Query: 268 RERRLLTLPVGV--LQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAG 317
           R R+LL        ++ DIVV+Q+G+G  KTI EA++  P+  N   ++++ AG
Sbjct: 253 RGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAG 291

BLAST of Pay0003489 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 112.1 bits (279), Expect = 8.4e-25
Identity = 86/292 (29.45%), Postives = 143/292 (48.97%), Query Frame = 0

Query: 28  RLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCV 87
           R ++IS  S+LLI +  V+ T  + +  +  A  D  +     +AI   C+ T +   C 
Sbjct: 15  RYVIISISSVLLI-SMVVAVTIGVSVNKSDNA-GDEEITTS-VKAIKDVCAPTDYKETCE 74

Query: 88  NSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLD 147
           ++L        ++  +LV  +FN T++ ++     S  +  LQ D R + A D C EL+D
Sbjct: 75  DTLRK-DAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELMD 134

Query: 148 DSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQM 207
            +I  +S+S + +         + L     +  WLSA ++++ TC DGF    G     +
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEAL---VKLRIWLSATISHEQTCLDGFQGTQGNAGETI 194

Query: 208 ADKLHNLSELVSNCLALFSGSETSDFAG---VPIQNKRRLMKEEGENEDSSGNFPRWMRG 267
              L    +L  N LA+   +E S++ G   +P  N RRL+ +E         FP WM  
Sbjct: 195 KKALKTAVQLTHNGLAMV--TEMSNYLGQMQIPEMNSRRLLSQE---------FPSWMDA 254

Query: 268 RERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSNRRTIIYVMAG 317
           R RRLL  P+  ++ DIVV+Q+G+G  KTI EA+   P+  N   ++++  G
Sbjct: 255 RARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEG 288

BLAST of Pay0003489 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 110.5 bits (275), Expect = 2.4e-24
Identity = 85/312 (27.24%), Postives = 155/312 (49.68%), Query Frame = 0

Query: 24  RSTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFP 83
           +   +L+L+S    LL VAA    +       AS A     L       +  +CS TR+P
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGIS-----AGASKANEKRTLSPSSHAVLRSSCSSTRYP 74

Query: 84  TLCVNSLLDFPGSLNANEQDLVHISFNITLQHLNKALYSSSEISSLQMDL--RVRSAYDA 143
            LC+++++   G    +++D++  S N+T+  +    ++  ++   +  L  R ++A   
Sbjct: 75  ELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHD 134

Query: 144 CLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSSEDIITWLSAALTNQDTCTDGFS--EL 203
           CLE +D+++D +  +++ +    +  T +    + D+ T +S+A+TNQ+TC DGFS  + 
Sbjct: 135 CLETIDETLDELHETVEDLHLYPTKKTLRE--HAGDLKTLISSAITNQETCLDGFSHDDA 194

Query: 204 SGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAG----VPIQNKRRLMKEE------- 263
              V+  +     ++  + SN LA+      +D A       I +  R +KEE       
Sbjct: 195 DKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVA 254

Query: 264 ----GENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQY 317
               G  E  S  +P W+   +RRL  L    ++AD  V+ +G+GT KT+A A+  AP+ 
Sbjct: 255 VDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFKTVAAAVAAAPEN 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3B07.0e-7755.59Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
Q9FK051.2e-7349.51Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q8GX861.4e-2429.25Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z81.2e-2329.45Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
O490063.4e-2327.24Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Match NameE-valueIdentityDescription
A0A5A7U3N71.8e-15599.33Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00145... [more]
A0A1S3CP111.8e-15599.33Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503123 PE=3 SV=1[more]
A0A5D3E0Z21.5e-15498.99Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G00370... [more]
A0A0A0L6S04.1e-15294.23Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1[more]
A0A6J1E4874.8e-13786.41Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0050503.13.6e-15599.33pectin methylesterase family protein [Cucumis melo var. makuwa][more]
XP_008465502.13.6e-15599.33PREDICTED: probable pectinesterase/pectinesterase inhibitor 34 [Cucumis melo][more]
TYK29180.13.1e-15498.99putative pectinesterase/pectinesterase inhibitor 34 [Cucumis melo var. makuwa][more]
XP_004146219.18.4e-15294.23probable pectinesterase/pectinesterase inhibitor 34 [Cucumis sativus] >KGN57675.... [more]
XP_038881267.13.3e-14090.60probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G49220.15.0e-7855.59Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.18.8e-7549.51pectin methylesterase PCR fragment F [more]
AT3G05610.19.9e-2629.25Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.18.4e-2529.45Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14310.12.4e-2427.24pectin methylesterase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 68..224
e-value: 3.4E-43
score: 159.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 46..225
e-value: 1.3E-37
score: 126.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 72..224
e-value: 7.4E-32
score: 110.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 280..316
e-value: 1.5E-6
score: 27.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 70..227
e-value: 2.9E-36
score: 126.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 71..226
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 232..317
e-value: 4.7E-7
score: 31.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 20..317
NoneNo IPR availablePANTHERPTHR31707:SF250PECTINESTERASEcoord: 20..317
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 76..226
e-value: 9.56463E-44
score: 145.274
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 277..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0003489.1Pay0003489.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity