Pay0002129 (gene) Melon (Payzawat) v1

Overview
NamePay0002129
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionL-ascorbate oxidase
Locationchr02: 3816516 .. 3821048 (+)
RNA-Seq ExpressionPay0002129
SyntenyPay0002129
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAAGTTGCAAATCAGCCATTGTTTCCTTTGCCTTTTCTAGTCTTCTTTGTTTTGTCAATCATATTTGGTTTTGGAATAACTCTCTCTAAGGCTGTTTCCCAAACCAGATACTACAAATTCGAAGTTGACTACATGTTTTGGTCCCCGGATTGTGTTGAAAACATCGTCTTGGGAATCAATGGTACATTTCCCGGTCCACCGATTAGAGCCAATGTTAATGACACCGTGGTTGTGGAGGTGATCAACAATCTCTCCACCGAAGGTGTCGTTATTCACTGGCATGGAATTCTGCAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCTCAGTGTCCCATTAATCCAGGAGAAAACTTCACTTACGAGTTCAAAGTGGATAAGGTGAGAAGGATTTAAAATGTCAATATCGATGGAAGTGTGTCATAGTTATATTTATGATAAAATCTATTCATTTGTTGATTTTTTTTCTTTTTGTAGCCTGGGACATATTTCTATCATGGTCATTTTGGGATGCAGAGAGCGGCTGGGTTGTATGGATCCTTAATAGTGGATTTAGAGGAAGGAAAGAAAGAGCCCTTCCAATATGACGAAGAGATCAACTTATTACTTAGTGATTGGTGGCATACAAGCATTCAGGAGCAAGAAGTTGGCCTCAAGTCAAATCCAATGCGCTGGATTGGAGAGCTCCAGGTAGTAAATTATGTTCATATTGACAACTAATATAGGTTTCTTAGTTAAATTAAAAAGACAAAATTATTTCTCATTTTTTTTCAAAATTTGACATATATTTGAAACATTAGTAGAAAGTAGATAATAAACTAGAGAAACATAACGTGAAAAAAAAAGTATTTTTAAGCTTAACAATTATACAAAAAAAAATTGTATATCTTTTGGGAACTATTTGATCTTTTTGTTTTTGTTTTGTCAAAGTAGGTTGCTTTCGTGATCCTCATTTAACAACATACGAAGAAAAGTGGTTTTTTGAAAACTATTACTTTTGAGTTTCCAAAATTTAACTTGGTTTATGAAAACTTATGACAGAAAATGTATAACAAAGCAAGAATTTAGAAGTAGAAGAGTTATTTATAGGCTTAACTTTCAAAGATCAAACATTTAATTCATTCTTTTCTTTTTTAGAAAAATTGAAAACTAAGTTTATTTGATTCCTAAAGTTTCAAAACGAAAAATTTAATATACCTGAATGTTTGAGAAACAATGACTATTTAGTCAGCTGGTTGATTAATGGTAAAATGAGTTTAATTATGCTTCATTTACTTCTTTTCACTTTGACATTTTTTATTTACAAACCCCATCTCCTTCCTTCTTCTCCATCGCCTTCTTCAACCTTACCTCCTCCTCAAGGTCTCTTTCAATAATCCTCAAACCATTGCTCAGATTTTCACAAAATCTATTTGAACTTTAATTTCGGCTTCATATCTTTACTTATGTTCCGATTCATTAGACGACGCTAGCACAAACTTAAAAAAGAGTTATTGTATCCTCTTACCAACACTTCATAAACATAGAAGAAGAGAGCAAAAAAGAGAAAAGCCAACATCATTTAGAATCAAAACATTCCTTTTAATACTGAAGTAAAGGAGATTGCCTAATCAGCTAGCAAACTCATTTTTTAACATTTTTCACACCATTTTTTCCTTAGTTAACATTTAAAATCATTTCTTAAACTTTATAGAATAAAGTTTTCATTTCAAAATTTCGAGACCAAACATACGTCAAGTTTGTCATTTTAATTTTTAGTCAATTTTTGAATTGAGAAAATATCAACTAAAATTTTAGCTCAAATTCAAAGGACAATAATTTTATCTCAAACTAGAAATATCATCGCAGAATTTGATAAAATATTCGAACTTTGTAATAAAATTGATTTTTGTCATGAAGTATAACTATTTTGTTTTTTTTTTATTTATTTATTATATTATTCTTAAAAAAACCCATAAATAATAGGAAAACCTAGAGTTTAATAATCCAATTATTAATAATCCAATCATGTTAGGCCTACCCATTTCTTAAGGATGGGCAGTGTCTCCGAATTTTGAATTTTATAATAATTTGTAGGGAAATTCCATGCAACATACTTTTCAAAATTTATATAAATCACATAATTTAAATCTAATTGAAAAAACATTAGAGATAACGGGTGGGCCACCAAAATTACTCGCATTCGGAAATAGATAATGATATTTTGTTATATATTTGTATATATTTCAAATTTTGGGATTTAAAATAATTATCCTTTAAACTAACTCATCTTATATATAATTAGTTGTCTCATTCATATTTATCGATAACTATAATTGATAGAACTTAAAATTTTAATTTTTTTTACAATTTTGCTCTAATAAACAAAGCTTAGATTATATTTGACCGTATGAGAAAGTAGATAATAATTAAAAATTGGTTGATATTTTTTTTCTTGAAATTTGTCATTAACGTTTGTTTGATTTAGGTAAAATACTTGAGATGTTTAAGTATAATGTTTTTTTTTCCTTTTTGTCTCTTGTTCTTGAAGGTCATACTCATAAATGGGACAGGGCAATTTAATTGTACAAGTTTAGATAAATACAACACGAGTTCGGAGCATTGTGAGTGGAAAGATCAATGTGCACCATTTGTCCTCAAAGTCGAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTATCTGCTCTCAATTTTGCCATTGCGGTAACACAACCTCTCACTTAGTTCTTTTTACAACAATTATAGAAATAAAAAATTGAATCGAATATTCTACCTCAAGAATGAAAAGTTATATCTGTTAGCTCTATGTTTATCCTTGACTTTTGAGATGTATGTCTATTTAAGTAAATATTCGAATTGTTCATTTATCACACGTAACAAAAAATGAGACTTAAAAATATAATAAATTGTGAATTTAGATTTTCAAGTGATTGGACTTAGATTTAGAAGGAAAAAAAATATTATTTTCAAATATAACAAAATAAACCAAAATAACTGTATGTTTATTAGTTTATGGTATTTATAAATATTTAAAAAAAATTGTCATTTAAAATAATTTTTCGAAAAAGAATAACCGACATGCATATATAATTTTTTTGAAAAATAAATCATACCCCAAATTATATATTCGACCATAATCTCAAAATTTTATGAAATAAATTTTGGGTTCTTTTTTTTTTTTTTTTTCTAACCATCTTAAAATAAAAAGTTTTAATACTTTTTCTTTTCTTTTCCAGAATCACACAATGTTGGTGGTAGAAGCCGACGGTAACTACGTTGACCCATTTGAAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCTGTTCTCGTCACCACTAACCAAGCCACAACCCAAAACTACTGGGCTTCCATAGGCGTCCGTGGTCGGAAGCCTAAAACATCGCCGGCACTCCTCCTCTTCAACTACCTTCCCAACTCCGTCGCCAAATTACCCATTTCTCCACCGCCAGAAACCCCCAAATGGGATGATTACGATCGAAGTAAAAGCTTCACAAACAGAGTCTTAGCCTCTGCCATGAACACAACAAAACCACCACCAATCTTCACTCGCCGGATCTCCCTCCTCAACACCCAAAATCGTATTAACGGCAAATTCACAAAATGGGCAATCAACAACATATCATTAACCCTCCCCTCAACCCCGTACCTCGGCGCCATTAAATTCGGTCTCAACTCCACCTTCAACCAGACTCCGCCACCAGAAACATTCCCAAAGAACTACAACATCAAAATCCCACCGCCGAACCCGAACACGAATACCGGAAACGGCGTTTATCGGTTCCGATCGGGGGAAATAGTGGACGTGATTCTACAAAACGCCAATGCGTTAACAAACAATACAAGTGAAATTCACCCTTGGCATTTACACGGCCATGATTTTTGGGTTTTGGGGTTTGGAGAGGGGAAATTTTCCGCCGCAGATGAGACGAAATTGAATCTGAAAAACCCACCGTTGAAGAACACGGTAGTGATTTTCCCATTTGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCCGGCGTTTGGATATTCCATTGCCACATTGAACCTCATTTGGATATGGGAATGGGGGTTATCTTCGCCGAAGCAGTTGAAAGGGTCAGGGAAATTCCGCCGTATGCTTTGGCTTGCGGCCGCACTGCCACACATAACGTTGTTCCAATAATCCCACAACCCAAACCACCCAGAGGACCCGGACCAGTTATCCCCTACTTCATGCTTTGGGTAATTGGGTTAGTTATAATTGTGTGTGGGATTGGACTGTTTGGTGTCTGTCTTTATCGGATTGGATTGCGTCGGGGTTATACTTTTAATAATTTGAGCTGGAGACCTTATTTGGGCCTTTCCGATAGTCAATCAAACCAAATTGAATTTTCAGATTTGTCTCGGTCCAACCTAACTCAAGAAGAGTCCAACCCAACCCAAGAAGAGTCCAACCCAACTCAAGAAGAGTCCAACCCTATTCTAGAAGAGCCCTAA

mRNA sequence

ATGGCAAAAGTTGCAAATCAGCCATTGTTTCCTTTGCCTTTTCTAGTCTTCTTTGTTTTGTCAATCATATTTGGTTTTGGAATAACTCTCTCTAAGGCTGTTTCCCAAACCAGATACTACAAATTCGAAGTTGACTACATGTTTTGGTCCCCGGATTGTGTTGAAAACATCGTCTTGGGAATCAATGGTACATTTCCCGGTCCACCGATTAGAGCCAATGTTAATGACACCGTGGTTGTGGAGGTGATCAACAATCTCTCCACCGAAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCTCAGTGTCCCATTAATCCAGGAGAAAACTTCACTTACGAGTTCAAAGTGGATAAGCCTGGGACATATTTCTATCATGGTCATTTTGGGATGCAGAGAGCGGCTGGGTTGTATGGATCCTTAATAGTGGATTTAGAGGAAGGAAAGAAAGAGCCCTTCCAATATGACGAAGAGATCAACTTATTACTTAGTGATTGGTGGCATACAAGCATTCAGGAGCAAGAAGTTGGCCTCAAGTCAAATCCAATGCGCTGGATTGGAGAGCTCCAGGTCATACTCATAAATGGGACAGGGCAATTTAATTGTACAAGTTTAGATAAATACAACACGAGTTCGGAGCATTGTGAGTGGAAAGATCAATGTGCACCATTTGTCCTCAAAGTCGAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTATCTGCTCTCAATTTTGCCATTGCGAATCACACAATGTTGGTGGTAGAAGCCGACGGTAACTACGTTGACCCATTTGAAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCTGTTCTCGTCACCACTAACCAAGCCACAACCCAAAACTACTGGGCTTCCATAGGCGTCCGTGGTCGGAAGCCTAAAACATCGCCGGCACTCCTCCTCTTCAACTACCTTCCCAACTCCGTCGCCAAATTACCCATTTCTCCACCGCCAGAAACCCCCAAATGGGATGATTACGATCGAAGTAAAAGCTTCACAAACAGAGTCTTAGCCTCTGCCATGAACACAACAAAACCACCACCAATCTTCACTCGCCGGATCTCCCTCCTCAACACCCAAAATCGTATTAACGGCAAATTCACAAAATGGGCAATCAACAACATATCATTAACCCTCCCCTCAACCCCGTACCTCGGCGCCATTAAATTCGGTCTCAACTCCACCTTCAACCAGACTCCGCCACCAGAAACATTCCCAAAGAACTACAACATCAAAATCCCACCGCCGAACCCGAACACGAATACCGGAAACGGCGTTTATCGGTTCCGATCGGGGGAAATAGTGGACGTGATTCTACAAAACGCCAATGCGTTAACAAACAATACAAGTGAAATTCACCCTTGGCATTTACACGGCCATGATTTTTGGGTTTTGGGGTTTGGAGAGGGGAAATTTTCCGCCGCAGATGAGACGAAATTGAATCTGAAAAACCCACCGTTGAAGAACACGGTAGTGATTTTCCCATTTGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCCGGCGTTTGGATATTCCATTGCCACATTGAACCTCATTTGGATATGGGAATGGGGGTTATCTTCGCCGAAGCAGTTGAAAGGGTCAGGGAAATTCCGCCGTATGCTTTGGCTTGCGGCCGCACTGCCACACATAACGTTGTTCCAATAATCCCACAACCCAAACCACCCAGAGGACCCGGACCAGTTATCCCCTACTTCATGCTTTGGGTAATTGGGTTAGTTATAATTGTGTGTGGGATTGGACTGTTTGGTGTCTGTCTTTATCGGATTGGATTGCGTCGGGGTTATACTTTTAATAATTTGAGCTGGAGACCTTATTTGGGCCTTTCCGATAGTCAATCAAACCAAATTGAATTTTCAGATTTGTCTCGGTCCAACCTAACTCAAGAAGAGTCCAACCCAACCCAAGAAGAGTCCAACCCAACTCAAGAAGAGTCCAACCCTATTCTAGAAGAGCCCTAA

Coding sequence (CDS)

ATGGCAAAAGTTGCAAATCAGCCATTGTTTCCTTTGCCTTTTCTAGTCTTCTTTGTTTTGTCAATCATATTTGGTTTTGGAATAACTCTCTCTAAGGCTGTTTCCCAAACCAGATACTACAAATTCGAAGTTGACTACATGTTTTGGTCCCCGGATTGTGTTGAAAACATCGTCTTGGGAATCAATGGTACATTTCCCGGTCCACCGATTAGAGCCAATGTTAATGACACCGTGGTTGTGGAGGTGATCAACAATCTCTCCACCGAAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCTCAGTGTCCCATTAATCCAGGAGAAAACTTCACTTACGAGTTCAAAGTGGATAAGCCTGGGACATATTTCTATCATGGTCATTTTGGGATGCAGAGAGCGGCTGGGTTGTATGGATCCTTAATAGTGGATTTAGAGGAAGGAAAGAAAGAGCCCTTCCAATATGACGAAGAGATCAACTTATTACTTAGTGATTGGTGGCATACAAGCATTCAGGAGCAAGAAGTTGGCCTCAAGTCAAATCCAATGCGCTGGATTGGAGAGCTCCAGGTCATACTCATAAATGGGACAGGGCAATTTAATTGTACAAGTTTAGATAAATACAACACGAGTTCGGAGCATTGTGAGTGGAAAGATCAATGTGCACCATTTGTCCTCAAAGTCGAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTATCTGCTCTCAATTTTGCCATTGCGAATCACACAATGTTGGTGGTAGAAGCCGACGGTAACTACGTTGACCCATTTGAAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCTGTTCTCGTCACCACTAACCAAGCCACAACCCAAAACTACTGGGCTTCCATAGGCGTCCGTGGTCGGAAGCCTAAAACATCGCCGGCACTCCTCCTCTTCAACTACCTTCCCAACTCCGTCGCCAAATTACCCATTTCTCCACCGCCAGAAACCCCCAAATGGGATGATTACGATCGAAGTAAAAGCTTCACAAACAGAGTCTTAGCCTCTGCCATGAACACAACAAAACCACCACCAATCTTCACTCGCCGGATCTCCCTCCTCAACACCCAAAATCGTATTAACGGCAAATTCACAAAATGGGCAATCAACAACATATCATTAACCCTCCCCTCAACCCCGTACCTCGGCGCCATTAAATTCGGTCTCAACTCCACCTTCAACCAGACTCCGCCACCAGAAACATTCCCAAAGAACTACAACATCAAAATCCCACCGCCGAACCCGAACACGAATACCGGAAACGGCGTTTATCGGTTCCGATCGGGGGAAATAGTGGACGTGATTCTACAAAACGCCAATGCGTTAACAAACAATACAAGTGAAATTCACCCTTGGCATTTACACGGCCATGATTTTTGGGTTTTGGGGTTTGGAGAGGGGAAATTTTCCGCCGCAGATGAGACGAAATTGAATCTGAAAAACCCACCGTTGAAGAACACGGTAGTGATTTTCCCATTTGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCCGGCGTTTGGATATTCCATTGCCACATTGAACCTCATTTGGATATGGGAATGGGGGTTATCTTCGCCGAAGCAGTTGAAAGGGTCAGGGAAATTCCGCCGTATGCTTTGGCTTGCGGCCGCACTGCCACACATAACGTTGTTCCAATAATCCCACAACCCAAACCACCCAGAGGACCCGGACCAGTTATCCCCTACTTCATGCTTTGGGTAATTGGGTTAGTTATAATTGTGTGTGGGATTGGACTGTTTGGTGTCTGTCTTTATCGGATTGGATTGCGTCGGGGTTATACTTTTAATAATTTGAGCTGGAGACCTTATTTGGGCCTTTCCGATAGTCAATCAAACCAAATTGAATTTTCAGATTTGTCTCGGTCCAACCTAACTCAAGAAGAGTCCAACCCAACCCAAGAAGAGTCCAACCCAACTCAAGAAGAGTCCAACCCTATTCTAGAAGAGCCCTAA

Protein sequence

MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEVINNLSTEGTPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSNLTQEESNPTQEESNPTQEESNPILEEP
Homology
BLAST of Pay0002129 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 1.9e-249
Identity = 420/589 (71.31%), Postives = 467/589 (79.29%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKA-VSQTRYYKFEVDYMFWSPDCVENIVL 60
           MAKVA++P FP PFL F VLSIIFGFGITLS+A   + ++YK++V+YMFWSPDCVENIV+
Sbjct: 1   MAKVADKPFFPKPFLSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVM 60

Query: 61  GINGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINP 120
           GING FPGP IRAN  D VVVE+ N L TE            GTPWADGTASISQC INP
Sbjct: 61  GINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINP 120

Query: 121 GENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWW 180
           GE FTY F VDK GTYFYHGH GMQR+AGLYGSLIVD  EG+ EPF YDEEINLLLSDWW
Sbjct: 121 GETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWW 180

Query: 181 HTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCE--WKDQCAPFV 240
           H S+ +QEVGL S PMRWIGE Q ILING GQF+C+   KYN   + CE   K++CAPF+
Sbjct: 181 HQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFI 240

Query: 241 LKVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVL 300
           L V+P KTYRIRIASTT+L++LNFAI NH +LVVEADGNYV PF TSDIDIYSGESYSVL
Sbjct: 241 LHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVL 300

Query: 301 VTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKS 360
           +TT+Q   +NYW SIGVR R PKT P L L NYLPNS +KLPISPPPETP W+D+DRSK+
Sbjct: 301 ITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKN 360

Query: 361 FTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFG 420
           FT R+ A AM + KPP  + RR+ LLNTQNRING F KWAINN+SL LP TPYL A+K  
Sbjct: 361 FTFRIFA-AMGSPKPPVRYNRRLFLLNTQNRING-FMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSE 480
           LN+ FNQ PPPETFP NY+I  PPPNP T TGNGVY+F  GE VDVILQNAN L  N SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGFGEGKFSA-ADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLG+GEGKF A  DE KLNLKNPPL+NTVVIFP+GWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIP 574
           W FHCHIEPHL MGMGV+FAE V  V  IPP ALACG TA     P +P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALVKNYPRLP 587

BLAST of Pay0002129 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 809.7 bits (2090), Expect = 2.5e-233
Identity = 388/565 (68.67%), Postives = 444/565 (78.58%), Query Frame = 0

Query: 14  FLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRAN 73
           FL+ F   +I  FGI  S   SQ R+YK+EV+YMFW+PDC ENIV+GING FPGP IRAN
Sbjct: 12  FLILFFFGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRAN 71

Query: 74  VNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPGENFTYEFKVDKPG 133
             DTVVVE+IN L TE            GTPWADGTASISQC INPGE F Y F VD PG
Sbjct: 72  AGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPG 131

Query: 134 TYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSN 193
           T+FYHGH GMQR+AGLYGSLIVD  +GKKEPF YD EINLLLSDWWH SI +QEVGL S 
Sbjct: 132 TFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSK 191

Query: 194 PMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIA 253
           P+RWIGE Q IL+NG GQF+C+   KY+++ E C+ K  + CAP++  V P KTYRIRIA
Sbjct: 192 PIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIA 251

Query: 254 STTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWAS 313
           STT+L+ALNFAI NH +LVVEADGNYV PF TSDIDIYSGESYSVL+TT+Q  ++NYW S
Sbjct: 252 STTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVS 311

Query: 314 IGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTK 373
           +G RGR P T P L L NYLPNSV+KLP SPPPETP WDD+DRSK+FT R+ A AM + K
Sbjct: 312 VGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITA-AMGSPK 371

Query: 374 PPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETF 433
           PP    RRI LLNTQN ING + KWAIN++SL LP TPYLGA+KF L   F+Q PPPE F
Sbjct: 372 PPVKSNRRIFLLNTQNVING-YVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVF 431

Query: 434 PKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVL 493
           P++Y+I  PP N  T  GNGVY+F+ GEIVDVILQNAN +  N SEIHPWHLHGHDFWVL
Sbjct: 432 PEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVL 491

Query: 494 GFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGM 553
           G+G+GKF+A +E+ LNLKNPPL+NTVVIFP+GWTAIRFVADNPGVW FHCHIEPHL MGM
Sbjct: 492 GYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGM 551

Query: 554 GVIFAEAVERVREIPPYALACGRTA 565
           GV+FAE VE+V  IP  ALACG TA
Sbjct: 552 GVVFAEGVEKVGRIPTKALACGGTA 572

BLAST of Pay0002129 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 6.4e-229
Identity = 378/558 (67.74%), Postives = 437/558 (78.32%), Query Frame = 0

Query: 35  SQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEVINNLSTE----- 94
           SQ R+YK+EV+YMFW+P+C ENIV+GING FPGP IRAN  D+VVVE+ N L TE     
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 95  -------GTPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLI 154
                  GTPWADGTASISQC INPGE F Y F VD PGT+FYHGH GMQR+AGLYGSLI
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 155 VDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNC 214
           VD  +GKKEPF YD EINLLLSDWWH SI +QEVGL S P+RWIGE Q IL+NG GQF+C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180

Query: 215 TSLDKYNTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLSALNFAIANHTMLVVE 274
           +   KY+++ E C+ K  + CAP++  V P KTYRIRIASTT+L+ALNFAI NH +LVVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240

Query: 275 ADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLP 334
           ADGNYV PF TSDIDIYSGESYSVL+TT+Q  ++NYW S+G R R P T P L L NYLP
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300

Query: 335 NSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGK 394
           NSV+KLP SPPP+TP WDD+DRSK+FT R+ A AM + KPP  F RRI LLNTQN ING 
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITA-AMGSPKPPVKFNRRIFLLNTQNVING- 360

Query: 395 FTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGV 454
           + KWAIN++SL LP TPYLGA+K+ L   F+Q PPPE FP++Y+I  PP N  T  GNGV
Sbjct: 361 YVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGV 420

Query: 455 YRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPP 514
           Y+F+ GE+VDVILQNAN +  N SE HPWHLHGHDFWVLG+G+GKFSA +E+ LNLKNPP
Sbjct: 421 YQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNPP 480

Query: 515 LKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALAC 574
           L+NTVVIFP+GWTAIRFVADNPGVW FHCHIEPHL MGMGV+FAE VE+V  IP  ALAC
Sbjct: 481 LRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALAC 540

Query: 575 GRTATHNVVPIIPQPKPP 579
           G TA      +I  PK P
Sbjct: 541 GGTAK----SLINNPKNP 552

BLAST of Pay0002129 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 1.3e-205
Identity = 345/569 (60.63%), Postives = 424/569 (74.52%), Query Frame = 0

Query: 12  LPFLVFFVLSIIFGFGITLSKAV--SQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPP 71
           L FL FF+L +I    ++ S++V  ++TR++K++V+Y+ WSPD  E++V+GING FPGP 
Sbjct: 4   LGFLFFFLLPLIL-LELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPT 63

Query: 72  IRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPGENFTYEFKV 131
           IRA   DTV V + N L TE            GTPWADGTA+ISQC INPGE F Y FKV
Sbjct: 64  IRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKV 123

Query: 132 DKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVG 191
           DK GTYFYHGH+GMQR+AGLYGSLIV++ EG+KEPF YD E NLLLSDWWH    EQEV 
Sbjct: 124 DKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVD 183

Query: 192 LKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSS-EHCEWK--DQCAPFVLKVEPNKTY 251
           L SNP+RWIGE Q +L+NG GQ+NC+   +++      C+ +  +Q AP +L+V PNK Y
Sbjct: 184 LSSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIY 243

Query: 252 RIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQ 311
           R+R+ASTT+L +L+ AI  H M+VVEADGNYV PF   D+DIYSGESYSVL  T+Q  T+
Sbjct: 244 RLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTK 303

Query: 312 NYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASA 371
           NYW SI VRGR+PKT   L L NYLPNS +K P  PPP  P W+DY+ SKSF+N++ A  
Sbjct: 304 NYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFA-L 363

Query: 372 MNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTP 431
           M + KPPP   RRI LLNTQN+I+G +TKWAINN+SL LP+  YLG+I++G+N+ F+  P
Sbjct: 364 MGSPKPPPQNHRRIILLNTQNKIDG-YTKWAINNVSLVLPTQLYLGSIRYGINA-FDTKP 423

Query: 432 PPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGH 491
           PP+ FPK+Y++    PN N+  GNGVY  +    +D+ILQNANAL  + SEIHPWHLHGH
Sbjct: 424 PPDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGH 483

Query: 492 DFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPH 551
           DFWVLG+GEGKFS  D  K NLKNPPL+NT VIFPFGWTA+RFV DNPGVW FHCHIEPH
Sbjct: 484 DFWVLGYGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPH 543

Query: 552 LDMGMGVIFAEAVERVREIPPYALACGRT 564
           L MGMGVIFAE V  V++IP  ALACG T
Sbjct: 544 LHMGMGVIFAEGVHLVKKIPKEALACGLT 568

BLAST of Pay0002129 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 2.4e-199
Identity = 328/557 (58.89%), Postives = 406/557 (72.89%), Query Frame = 0

Query: 23  IFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEV 82
           I    I    A +  R Y +EV+Y F  PDC E +V+ +NG FPGP I A   DT+VV +
Sbjct: 6   IVAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHL 65

Query: 83  INNLSTE------------GTPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFG 142
            N L+TE            G+PWADG A ++QC I+PGE FTY F VDKPGT+FYHGH+G
Sbjct: 66  TNKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYG 125

Query: 143 MQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGELQ 202
           MQR+AGLYGSLI+D+ +GKKEP +YD E NLLLSDWWH  +  QE+GL S PMRWIGE Q
Sbjct: 126 MQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQ 185

Query: 203 VILINGTGQFNCTSLDKY-NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLSAL 262
            ILING GQFNC+   ++ +TS   C +K  DQCAP  L VEPNKTYRIR+AS+T+L++L
Sbjct: 186 SILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASL 245

Query: 263 NFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVRGRKP 322
           NFA+  H ++VVEADGNY+ PF T DIDIYSGE+YSVL+TT+Q  +QNY+ + GVRGRKP
Sbjct: 246 NFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKP 305

Query: 323 KTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRR 382
            T PAL + NY+    ++LP SPPPETP+W+D+DRSK+F+ ++ A AM +  PP  F  R
Sbjct: 306 NTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFA-AMGSPSPPETFDER 365

Query: 383 ISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNYNIKI 442
           + LLNTQN I G FTKWAINN+SL +P TPYLG++K+ L + FN++ PP+ +P +Y+I  
Sbjct: 366 LILLNTQNLIEG-FTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMT 425

Query: 443 PPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGEGKFS 502
           PP N N   GN    F     VDVILQNAN L  N SEIHPWHLHGHDFWVLG+GEGKF 
Sbjct: 426 PPRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFK 485

Query: 503 -AADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEA 562
              DE   NLKNPPL+NTV ++P+GWTA+RFV DNPGVW FHCHIEPHL MGMGV+FAE 
Sbjct: 486 PGVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEG 545

Query: 563 VERVREIPPYALACGRT 564
           + R+ ++P  AL CG T
Sbjct: 546 LNRIGKVPDEALGCGLT 560

BLAST of Pay0002129 vs. ExPASy TrEMBL
Match: A0A1S3BQ56 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492477 PE=3 SV=1)

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 675/687 (98.25%), Postives = 675/687 (98.25%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTE            GTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660

Query: 661 LTQEESNPTQEESNPTQEESNPILEEP 676
           LTQEESNPTQEESNPTQEESNPILEEP
Sbjct: 661 LTQEESNPTQEESNPTQEESNPILEEP 687

BLAST of Pay0002129 vs. ExPASy TrEMBL
Match: Q9ZR59 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=ao3 PE=3 SV=1)

HSP 1 Score: 1383.2 bits (3579), Expect = 0.0e+00
Identity = 673/687 (97.96%), Postives = 674/687 (98.11%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTE            GTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFG+ KFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660

Query: 661 LTQEESNPTQEESNPTQEESNPILEEP 676
           LTQEESNPTQEESNPTQEESNPILEEP
Sbjct: 661 LTQEESNPTQEESNPTQEESNPILEEP 687

BLAST of Pay0002129 vs. ExPASy TrEMBL
Match: A0A0A0M2J6 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G602530 PE=3 SV=1)

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 587/685 (85.69%), Postives = 613/685 (89.49%), Query Frame = 0

Query: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTE            G+PWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLSALNFAIA-----------NHTMLVVEADGNYVDPFETSDID 300
           KVEPNKTYRIRIASTTSL+ALNFAI+           NHTMLVVEADGNYV  F TSDID
Sbjct: 241 KVEPNKTYRIRIASTTSLAALNFAISKKFHHFSFLFQNHTMLVVEADGNYVQQFVTSDID 300

Query: 301 IYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETP 360
           IYSGESYSVLVTTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE P
Sbjct: 301 IYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIP 360

Query: 361 KWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPS 420
           KW+DYDRSK+FT RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPS
Sbjct: 361 KWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPS 420

Query: 421 TPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQN 480
           TPYLGAIKFGLNSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQN
Sbjct: 421 TPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQN 480

Query: 481 ANALTNNTSEIHPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAI 540
           ANAL+NNTSEIHPWHLHGHDFWVLG+GEGKFS  DETKLNLKNPPLKNTVVIFPFGWTAI
Sbjct: 481 ANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAI 540

Query: 541 RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQP 600
           RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P
Sbjct: 541 RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRP 600

Query: 601 KPPRGPGPVIPYFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQ 660
            P   PG   PY+++W IGLVI +C IGL  VCLYRI       FNNLSWRPYLG+S+SQ
Sbjct: 601 NP---PGLDNPYYIIW-IGLVIFLCVIGLCYVCLYRI------RFNNLSWRPYLGISESQ 660

BLAST of Pay0002129 vs. ExPASy TrEMBL
Match: Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.4e-258
Identity = 433/578 (74.91%), Postives = 488/578 (84.43%), Query Frame = 0

Query: 3   KVANQPLFPLPF-LVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGI 62
           KVANQPLFP+PF  +FF+ SI FGFGITLS+A SQTR++K+EV+YMF SPDC EN+V+GI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPGE 122
           N  FPGP IRANV DTVVVE+IN LSTE            GTPWADGTASISQC INPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 NFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHT 182
           +FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH 
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCE--WKDQCAPFVLK 242
           S+ +QEVGL S+PMRWIGE Q ILING GQ NC+   KY  + E C+    ++CAPF+LK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVT 302
           V+PNKTYRIRI STTSLSALNFAI NH +LVVEADGNYV PF TSDIDIYSGESYSVL+ 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFT 362
           T+Q  +QNYW S+GVRGR+PKT P L L NYLPNS +KLPISPPPETPKW+DYDRSK+FT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 NRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLN 422
            R+LA AM + KPP   TRRI LLNTQN++NG +TKWAINN+SLTLPSTPYLGAIK G+N
Sbjct: 364 YRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIN 423

Query: 423 STFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIH 482
            TF+Q PPPET+P+NY+I  PPPN NT TGNGVYRF+ GEIVDV+LQNAN L +  SEIH
Sbjct: 424 DTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIH 483

Query: 483 PWHLHGHDFWVLGFGEGKFSAA-DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 542
           PWHLHGHDFWVLG+GEGKFSAA DETKLNLK+PPL+NTVVIFPFGWT +RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWA 543

Query: 543 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTA 565
           FHCHIEPHL MGMGV+FAE V++V  IP +A+ACG TA
Sbjct: 544 FHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of Pay0002129 vs. ExPASy TrEMBL
Match: A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 3.4e-257
Identity = 431/578 (74.57%), Postives = 486/578 (84.08%), Query Frame = 0

Query: 3   KVANQPLFPLPF-LVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGI 62
           K ANQPLFP+PF  +FF+ SI FGFGITLS+A SQTR++K+EV+YMF SPDC EN+V+GI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPGE 122
           N  FPGP IRANV DT+VVE+IN LSTE            GTPWADGTASISQC INPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 NFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHT 182
           +FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH 
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCE--WKDQCAPFVLK 242
           S+ +QEVGL S+PMRWIGE Q ILING GQ NC+   KY  + E C+    + CAPF+LK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVT 302
           V+PNKTYRIRI STTSLSALNFAI NH +LVVEADGNYV PF TSDIDIYSGESYSVL+ 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFT 362
           T+Q  +QNYW S+GVRGR+PKT P L L NYLPNS +KLPISPPPETPKW+DYDRSK+FT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 NRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLN 422
            R+LA AM + KPP   TRRI LLNTQN++NG +TKWAINN+SLTLPSTPYLGAIK G+N
Sbjct: 364 YRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIN 423

Query: 423 STFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIH 482
            TF+Q PPPET+P+NY+I  PPPN NT TGNGVYRF+ GEIVDV+LQNAN L +  SEIH
Sbjct: 424 DTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIH 483

Query: 483 PWHLHGHDFWVLGFGEGKFSAA-DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 542
           PWHLHGHDFWVLG+GEGKFSAA DETKLNLK+PPL+NTVVIFPFGWT +RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWA 543

Query: 543 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTA 565
           FHCHIEPHL MGMGV+FAE V++V  IP +A+ACG TA
Sbjct: 544 FHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of Pay0002129 vs. NCBI nr
Match: XP_008451098.1 (PREDICTED: L-ascorbate oxidase-like [Cucumis melo])

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 675/687 (98.25%), Postives = 675/687 (98.25%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTE            GTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660

Query: 661 LTQEESNPTQEESNPTQEESNPILEEP 676
           LTQEESNPTQEESNPTQEESNPILEEP
Sbjct: 661 LTQEESNPTQEESNPTQEESNPILEEP 687

BLAST of Pay0002129 vs. NCBI nr
Match: CAA71275.1 (L-ascorbate oxidase [Cucumis melo])

HSP 1 Score: 1383.2 bits (3579), Expect = 0.0e+00
Identity = 673/687 (97.96%), Postives = 674/687 (98.11%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTE            GTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFG+ KFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSRSN 660

Query: 661 LTQEESNPTQEESNPTQEESNPILEEP 676
           LTQEESNPTQEESNPTQEESNPILEEP
Sbjct: 661 LTQEESNPTQEESNPTQEESNPILEEP 687

BLAST of Pay0002129 vs. NCBI nr
Match: XP_011660094.1 (L-ascorbate oxidase-like isoform X1 [Cucumis sativus] >KAE8653486.1 hypothetical protein Csa_007106 [Cucumis sativus])

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 587/674 (87.09%), Postives = 613/674 (90.95%), Query Frame = 0

Query: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTE            G+PWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300
           KVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300

Query: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360

Query: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480

Query: 481 HPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+GEGKFS  DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P P   PG   P
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNP---PGLDNP 600

Query: 601 YFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSR 660
           Y+++W IGLVI +C IGL  VCLYRI       FNNLSWRPYLG+S+SQSNQIEFSDLS+
Sbjct: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI------RFNNLSWRPYLGISESQSNQIEFSDLSQ 660

BLAST of Pay0002129 vs. NCBI nr
Match: XP_031736736.1 (L-ascorbate oxidase-like isoform X2 [Cucumis sativus])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.9e-300
Identity = 529/674 (78.49%), Postives = 552/674 (81.90%), Query Frame = 0

Query: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTE            G+PWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GE Q                                    
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQ------------------------------------ 240

Query: 241 KVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300
                                     NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 --------------------------NHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300

Query: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360

Query: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480

Query: 481 HPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+GEGKFS  DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P P   PG   P
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNP---PGLDNP 599

Query: 601 YFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDSQSNQIEFSDLSR 660
           Y+++W IGLVI +C IGL  VCLYRI       FNNLSWRPYLG+S+SQSNQIEFSDLS+
Sbjct: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI------RFNNLSWRPYLGISESQSNQIEFSDLSQ 599

BLAST of Pay0002129 vs. NCBI nr
Match: NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])

HSP 1 Score: 902.1 bits (2330), Expect = 2.8e-258
Identity = 433/578 (74.91%), Postives = 488/578 (84.43%), Query Frame = 0

Query: 3   KVANQPLFPLPF-LVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGI 62
           KVANQPLFP+PF  +FF+ SI FGFGITLS+A SQTR++K+EV+YMF SPDC EN+V+GI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGTFPGPPIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPGE 122
           N  FPGP IRANV DTVVVE+IN LSTE            GTPWADGTASISQC INPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 NFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHT 182
           +FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH 
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCE--WKDQCAPFVLK 242
           S+ +QEVGL S+PMRWIGE Q ILING GQ NC+   KY  + E C+    ++CAPF+LK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVT 302
           V+PNKTYRIRI STTSLSALNFAI NH +LVVEADGNYV PF TSDIDIYSGESYSVL+ 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFT 362
           T+Q  +QNYW S+GVRGR+PKT P L L NYLPNS +KLPISPPPETPKW+DYDRSK+FT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 NRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLN 422
            R+LA AM + KPP   TRRI LLNTQN++NG +TKWAINN+SLTLPSTPYLGAIK G+N
Sbjct: 364 YRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIN 423

Query: 423 STFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIH 482
            TF+Q PPPET+P+NY+I  PPPN NT TGNGVYRF+ GEIVDV+LQNAN L +  SEIH
Sbjct: 424 DTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIH 483

Query: 483 PWHLHGHDFWVLGFGEGKFSAA-DETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 542
           PWHLHGHDFWVLG+GEGKFSAA DETKLNLK+PPL+NTVVIFPFGWT +RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWA 543

Query: 543 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTA 565
           FHCHIEPHL MGMGV+FAE V++V  IP +A+ACG TA
Sbjct: 544 FHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of Pay0002129 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 704.1 bits (1816), Expect = 1.1e-202
Identity = 331/565 (58.58%), Postives = 418/565 (73.98%), Query Frame = 0

Query: 16  VFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVN 75
           V++++ ++     T S AV   R Y +EV+Y +WSPDC E  V+ +NG FPGP I+A   
Sbjct: 19  VWWIVLVVAVLTHTASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAG 78

Query: 76  DTVVVEVINNLSTE------------GTPWADGTASISQCPINPGENFTYEFKVDKPGTY 135
           DT+VV + N L+TE            G+PWADG A ++QC INPGE FTY F V+KPGT+
Sbjct: 79  DTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTH 138

Query: 136 FYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPM 195
           FYHGH+GMQR+AGLYGSLIVD+ +GK E  +YD E NLLLSDWWH +I  QE+GL S PM
Sbjct: 139 FYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPM 198

Query: 196 RWIGELQVILINGTGQFNCTSLDKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIA 255
           RWIGE Q ILING GQFNC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++
Sbjct: 199 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 258

Query: 256 STTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWAS 315
           STT+L++LN A+  H ++VVEADGNY+ PF T DIDIYSGESYSVL+TT+Q  +QNY+ S
Sbjct: 259 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 318

Query: 316 IGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTK 375
           +GVRGRKP T+ AL + NY+    +KLP SPPP TP+WDD++RSK+F+ ++  SAM +  
Sbjct: 319 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPS 378

Query: 376 PPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETF 435
           PP  + +R+ LLNTQN I+G +TKWAINN+SL  P+TPYLG++K+ L   FN+  PP ++
Sbjct: 379 PPKKYRKRLILLNTQNLIDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSY 438

Query: 436 PKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVL 495
             +Y+I  PPP PNT TGNG+Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVL
Sbjct: 439 RMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVL 498

Query: 496 GFGEGKFS-AADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMG 555
           G+G+GKF    DE   NLKNPPL+NT +++P+GWTAIRFV DNPGVW FHCHIEPHL MG
Sbjct: 499 GYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMG 558

Query: 556 MGVIFAEAVERVREIPPYALACGRT 564
           MGV+FAE + R+ ++P  AL CG T
Sbjct: 559 MGVVFAEGLNRIGKVPDEALGCGLT 578

BLAST of Pay0002129 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 656.0 bits (1691), Expect = 3.3e-188
Identity = 322/569 (56.59%), Postives = 404/569 (71.00%), Query Frame = 0

Query: 15  LVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANV 74
           +V+++L+++     + S AV ++ +   EV+Y +W PDC E IV+ ING FPGP I A  
Sbjct: 4   IVWWLLTVVVVAFHSASAAVVESTW---EVEYKYWWPDCKEGIVMAINGQFPGPTIDAVA 63

Query: 75  NDTVVVEVINNLSTE------------GTPWADGTASISQCPINPGENFTYEFKVDKPGT 134
            DTV++ V+N LSTE            GTPWADG A ++QCPINPGE FTY+F VDK GT
Sbjct: 64  GDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGT 123

Query: 135 YFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNP 194
           +FYHGH+GMQR++GLYG LIV      KE   YD E NLLLSDWWH SI  QE+ L S P
Sbjct: 124 HFYHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSRP 183

Query: 195 MRWIGELQVILINGTGQFNCTSLDKYNTSSEH--CEWK--DQCAPFVLKVEPNKTYRIRI 254
           MRWIGE Q +LING GQFNC+    +N   E   C +K  DQCAP  L+VEPN+ YR+RI
Sbjct: 184 MRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRI 243

Query: 255 ASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWA 314
           ASTT+L++LN A+  H ++VVEADGNYV PF  +DID+YSGE+YSVL+ TN   ++ YW 
Sbjct: 244 ASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWI 303

Query: 315 SIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTT 374
           S+GVRGR+PKT  AL + NY+ ++    P  PPP TP W+D DRSKSF+ ++ A A    
Sbjct: 304 SVGVRGREPKTPQALTVINYV-DATESRPSHPPPVTPIWNDTDRSKSFSKKIFA-AKGYP 363

Query: 375 KPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFN-QTPPPE 434
           KPP     ++ LLNTQN +   +TKW+INN+SL++P TPYLG+I++GL S ++ ++P  +
Sbjct: 364 KPPEKSHDQLILLNTQN-LYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKK 423

Query: 435 TFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFW 494
               NY+I  PPPNPNT  G+G+Y F  G +VDVILQNAN L    SEIHPWH+HGHDFW
Sbjct: 424 LIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFW 483

Query: 495 VLGFGEGKFS-AADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLD 554
           VLG+GEGKF    DE   NLKNPPL+NTVV++PFGWTAIRFV DNPGVW FHCHIEPHL 
Sbjct: 484 VLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLH 543

Query: 555 MGMGVIFAEAVERV--REIPPYALACGRT 564
           MGMGV+F E V+R+   EIP  AL CG T
Sbjct: 544 MGMGVVFVEGVDRIGKMEIPDEALGCGLT 563

BLAST of Pay0002129 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 642.1 bits (1655), Expect = 5.0e-184
Identity = 305/525 (58.10%), Postives = 387/525 (73.71%), Query Frame = 0

Query: 16  VFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVN 75
           V++++ ++     T S AV   R Y +EV+Y +WSPDC E  V+ +NG FPGP I+A   
Sbjct: 19  VWWIVLVVAVLTHTASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAG 78

Query: 76  DTVVVEVINNLSTE------------GTPWADGTASISQCPINPGENFTYEFKVDKPGTY 135
           DT+VV + N L+TE            G+PWADG A ++QC INPGE FTY F V+KPGT+
Sbjct: 79  DTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTH 138

Query: 136 FYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPM 195
           FYHGH+GMQR+AGLYGSLIVD+ +GK E  +YD E NLLLSDWWH +I  QE+GL S PM
Sbjct: 139 FYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPM 198

Query: 196 RWIGELQVILINGTGQFNCTSLDKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIA 255
           RWIGE Q ILING GQFNC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++
Sbjct: 199 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 258

Query: 256 STTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWAS 315
           STT+L++LN A+  H ++VVEADGNY+ PF T DIDIYSGESYSVL+TT+Q  +QNY+ S
Sbjct: 259 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 318

Query: 316 IGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTK 375
           +GVRGRKP T+ AL + NY+    +KLP SPPP TP+WDD++RSK+F+ ++  SAM +  
Sbjct: 319 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPS 378

Query: 376 PPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETF 435
           PP  + +R+ LLNTQN I+G +TKWAINN+SL  P+TPYLG++K+ L   FN+  PP ++
Sbjct: 379 PPKKYRKRLILLNTQNLIDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSY 438

Query: 436 PKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVL 495
             +Y+I  PPP PNT TGNG+Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVL
Sbjct: 439 RMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVL 498

Query: 496 GFGEGKFS-AADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPG 524
           G+G+GKF    DE   NLKNPPL+NT +++P+GWTAIRFV DNPG
Sbjct: 499 GYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

BLAST of Pay0002129 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 503.8 bits (1296), Expect = 2.1e-142
Identity = 259/577 (44.89%), Postives = 349/577 (60.49%), Query Frame = 0

Query: 9   LFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGP 68
           L  L F+  F  S++            + R +K+EV Y F SPDC E +V+ ING FPGP
Sbjct: 16  LMVLCFIALFFSSVL---------CQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGP 75

Query: 69  PIRANVNDTVVVEVINNLSTE------------GTPWADGTASISQCPINPGENFTYEFK 128
            I+A   DT+VVE+ N+  TE            GTPW DG   ++QCPI PGE F Y+F 
Sbjct: 76  TIKAQQGDTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFV 135

Query: 129 VDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEV 188
           VD+PGTY YH H+GMQR +GL G + V     + EPF YD + N LL+DW+H S+ E+  
Sbjct: 136 VDRPGTYMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKAT 195

Query: 189 GLKSNPMRWIGELQVILINGTGQFNCTS--LDKYNTSSEHCEWKD-QCAPFVLKVEPNKT 248
           GL S P +W+GE Q ++I G G+FNC++      +  S  C   +  C+ F+L V P KT
Sbjct: 196 GLASIPFKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKT 255

Query: 249 YRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATT 308
           YR+RI S T+LSAL+F I  H + VVEADG+YV+PF   ++ +YSGE+YSVL+  +Q   
Sbjct: 256 YRLRIGSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPR 315

Query: 309 QNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPET----PKWDDYDRSKSFTNR 368
           +NYW +  +  R   T PA  + NY PN   + P  P  E+    P+W+D     + +  
Sbjct: 316 RNYWITSSIVSRPATTPPATAVLNYYPNHPRRRP--PTSESSNIVPEWNDTRSRLAQSLA 375

Query: 369 VLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNST 428
           + A        P    + I LLNTQN +NG + +W++NN+S   P TPYL A+K  L + 
Sbjct: 376 IKARRGFIHALPENSDKVIVLLNTQNEVNG-YRRWSVNNVSYHHPKTPYLIALKQNLTNA 435

Query: 429 FNQ--TPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIH 488
           F+   T P     +NY+I   P N N  T +G+YR R    VDVILQNAN +  N SE H
Sbjct: 436 FDWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETH 495

Query: 489 PWHLHGHDFWVLGFGEGKFSAADETK-LNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 548
           PWHLHGHDFWVLG+GEGKF+ +++ K  N  +P  KNTV + PFGWTA+RF ADNPGVW 
Sbjct: 496 PWHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWS 555

Query: 549 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRT 564
           FHCHIE H  MGMG++F   +++V  +P   + CG+T
Sbjct: 556 FHCHIESHFFMGMGIVFESGIDKVSSLPSSIMGCGQT 580

BLAST of Pay0002129 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 488.4 bits (1256), Expect = 9.1e-138
Identity = 228/381 (59.84%), Postives = 290/381 (76.12%), Query Frame = 0

Query: 188 ELQVILINGTGQFNCTSLDKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTS 247
           +L+ ILING GQFNC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++STT+
Sbjct: 9   KLKSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTA 68

Query: 248 LSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVR 307
           L++LN A+  H ++VVEADGNY+ PF T DIDIYSGESYSVL+TT+Q  +QNY+ S+GVR
Sbjct: 69  LASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVR 128

Query: 308 GRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPI 367
           GRKP T+ AL + NY+    +KLP SPPP TP+WDD++RSK+F+ ++  SAM +  PP  
Sbjct: 129 GRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPSPPKK 188

Query: 368 FTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNY 427
           + +R+ LLNTQN I+G +TKWAINN+SL  P+TPYLG++K+ L   FN+  PP ++  +Y
Sbjct: 189 YRKRLILLNTQNLIDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDY 248

Query: 428 NIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGE 487
           +I  PPP PNT TGNG+Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVLG+G+
Sbjct: 249 DIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGD 308

Query: 488 GKFS-AADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVI 547
           GKF    DE   NLKNPPL+NT +++P+GWTAIRFV DNPGVW FHCHIEPHL MGMGV+
Sbjct: 309 GKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVV 368

Query: 548 FAEAVERVREIPPYALACGRT 564
           FAE + R+ ++P  AL CG T
Sbjct: 369 FAEGLNRIGKVPDEALGCGLT 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P141331.9e-24971.31L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P247922.5e-23368.67L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2[more]
P370646.4e-22967.74L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1[more]
Q405881.3e-20560.63L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1[more]
M4DUF22.4e-19958.89L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BQ560.0e+0098.25L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492477 PE=3 SV=1[more]
Q9ZR590.0e+0097.96L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=ao3 PE=3 SV=1[more]
A0A0A0M2J60.0e+0085.69L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G602530 PE=3 SV=1[more]
Q9M5B51.4e-25874.91L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1[more]
A0A5D3CF113.4e-25774.57L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G0... [more]
Match NameE-valueIdentityDescription
XP_008451098.10.0e+0098.25PREDICTED: L-ascorbate oxidase-like [Cucumis melo][more]
CAA71275.10.0e+0097.96L-ascorbate oxidase [Cucumis melo][more]
XP_011660094.10.0e+0087.09L-ascorbate oxidase-like isoform X1 [Cucumis sativus] >KAE8653486.1 hypothetical... [more]
XP_031736736.13.9e-30078.49L-ascorbate oxidase-like isoform X2 [Cucumis sativus][more]
NP_001284440.12.8e-25874.91L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucum... [more]
Match NameE-valueIdentityDescription
AT5G21105.11.1e-20258.58Plant L-ascorbate oxidase [more]
AT5G21100.13.3e-18856.59Plant L-ascorbate oxidase [more]
AT5G21105.35.0e-18458.10Plant L-ascorbate oxidase [more]
AT4G39830.12.1e-14244.89Cupredoxin superfamily protein [more]
AT5G21105.29.1e-13859.84Plant L-ascorbate oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 44..145
e-value: 1.4E-27
score: 96.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 153..356
e-value: 8.5E-61
score: 207.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 358..569
e-value: 1.2E-74
score: 252.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 28..144
e-value: 8.2E-36
score: 125.2
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 32..169
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 152..353
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 363..566
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 431..546
e-value: 1.7E-36
score: 125.0
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..319
e-value: 2.3E-47
score: 161.0
IPR017760L-ascorbate oxidase, plantsTIGRFAMTIGR03388TIGR03388coord: 38..563
e-value: 1.2E-263
score: 873.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 642..675
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 642..669
NoneNo IPR availablePANTHERPTHR11709:SF394PLANT L-ASCORBATE OXIDASEcoord: 31..564
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 31..564
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 523..543
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 528..539
IPR034267Ascorbate oxidase, third cupredoxin domainCDDcd13893CuRO_3_AAOcoord: 364..554
e-value: 4.71816E-78
score: 243.866

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0002129.1Pay0002129.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity