Pay0000710 (gene) Melon (Payzawat) v1

Overview
NamePay0000710
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptionfatty acid desaturase 4, chloroplastic
Locationchr06: 29955352 .. 29956293 (-)
RNA-Seq ExpressionPay0000710
SyntenyPay0000710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGATCCCATGTTAATCCATGCAGCACTCCGACTCCACGGAGAGTTGTCTACTGCATAGCTAATACCACAGTCAATGGTGTTGGTACCAGTTCGAAGCATGGTGATGGTCAACTAGTCATTGAACGTGAGGTTATATCTCCGAGTCCACTTACATCGGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGGTCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACGGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACGTATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCTGGAATTTATCACTGGGGCATCGATAACTATGGTGGTGCGTCAACCCCAATCTTTGGTACCCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCACTCTACATGGTTTTGTTGGTGTCTGCTCAGGTTGCATAATGTTTAGTCAACAGTTTCATGCATGGGCTCATGGTAAAAAGAGTGAAATTCCGCCGCTCGTGGTGGCAATGCAAGATGCTGGCCTGCTCGTGTCACGAACACAGCATGCTACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATCAGCTTTTGGATGAAACAAAGGTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

mRNA sequence

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGATCCCATGTTAATCCATGCAGCACTCCGACTCCACGGAGAGTTGTCTACTGCATAGCTAATACCACAGTCAATGGTGTTGGTACCAGTTCGAAGCATGGTGATGGTCAACTAGTCATTGAACGTGAGGTTATATCTCCGAGTCCACTTACATCGGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGGTCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACGGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACGTATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCTGGAATTTATCACTGGGGCATCGATAACTATGGTGGTGCGTCAACCCCAATCTTTGGTACCCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCACTCTACATGGTTTTGTTGGTGTCTGCTCAGGTTGCATAATGTTTAGTCAACAGTTTCATGCATGGGCTCATGGTAAAAAGAGTGAAATTCCGCCGCTCGTGGTGGCAATGCAAGATGCTGGCCTGCTCGTGTCACGAACACAGCATGCTACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATCAGCTTTTGGATGAAACAAAGGTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Coding sequence (CDS)

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGATCCCATGTTAATCCATGCAGCACTCCGACTCCACGGAGAGTTGTCTACTGCATAGCTAATACCACAGTCAATGGTGTTGGTACCAGTTCGAAGCATGGTGATGGTCAACTAGTCATTGAACGTGAGGTTATATCTCCGAGTCCACTTACATCGGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGGTCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACGGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACGTATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCTGGAATTTATCACTGGGGCATCGATAACTATGGTGGTGCGTCAACCCCAATCTTTGGTACCCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCACTCTACATGGTTTTGTTGGTGTCTGCTCAGGTTGCATAATGTTTAGTCAACAGTTTCATGCATGGGCTCATGGTAAAAAGAGTGAAATTCCGCCGCTCGTGGTGGCAATGCAAGATGCTGGCCTGCTCGTGTCACGAACACAGCATGCTACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATCAGCTTTTGGATGAAACAAAGGTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Protein sequence

MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEWTEETEILEQTTAH
Homology
BLAST of Pay0000710 vs. ExPASy Swiss-Prot
Match: Q9SZ42 (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 2.9e-107
Identity = 184/292 (63.01%), Postives = 219/292 (75.00%), Query Frame = 0

Query: 23  PTPRRVVYCIANTTVN--------GVGTSSKHGDGQLVIEREVISPSPLTSVNSSTYYSV 82
           P P R    +   TVN           T  +    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALAALVGYILADLGSGI 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   S + LEPALA   GYILADLGSG+
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYG  STP+ GTQIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLLVSRTQHATHHRPPY 262
           +DP  HGFV   + CI+FSQQFHAWAHG KS++PPLVVA+QD GLLVSR QHA HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+KVFEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

BLAST of Pay0000710 vs. ExPASy Swiss-Prot
Match: O04584 (Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L1 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.7e-97
Identity = 170/305 (55.74%), Postives = 217/305 (71.15%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           M++  Q K  L   +++     PT  RV  C   T      T+ +     LV+E+ +++P
Sbjct: 1   MAVSFQTKNPLRPITNIPRSYGPTRVRVT-CSVTT------TNPQLNHENLVVEKRLVNP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
            PL         S  NDP+L+STW+HR WVA G TT+  S AKSI+G   S +WL+PALA
Sbjct: 61  -PL---------SKNNDPTLQSTWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
              GY+ ADLGSG+YHW IDNYGGASTPI G Q+EA QGHHK+PWTITKRQFANN Y +A
Sbjct: 121 CYAGYVFADLGSGVYHWAIDNYGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RA+TF VLP++LA ++P  H FV   + CI+ SQQFHAWAHG KS++PPLV+A+QD GLL
Sbjct: 181 RAITFIVLPLNLAINNPLFHSFVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSR  H  HH+ PYN+NYC+VSG WN++LDE+ +F+ALEM L+F+ G+RP SW EPNS+W
Sbjct: 241 VSRKDHPGHHQAPYNSNYCVVSGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDW 288

Query: 301 TEETE 306
           TEETE
Sbjct: 301 TEETE 288

BLAST of Pay0000710 vs. ExPASy Swiss-Prot
Match: O81006 (Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L2 PE=2 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 7.4e-95
Identity = 163/291 (56.01%), Postives = 209/291 (71.82%), Query Frame = 0

Query: 11  LSSRSHVNPCSTPTPR--RVVYCIANTTVNGVGTSSKHGDGQLVIEREVISPSPLTSVNS 70
           L ++  +NP +   PR  R  +    +T N    S  + + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN----SQPNHEMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  S +WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSG+YHW  DNYG  STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLLVSRTQHAT 250
           P+ LAFDD  +HGFV + + C++F Q FHAWAHG KS++PPLVV +QD GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WN++LDE+ VF+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

BLAST of Pay0000710 vs. ExPASy Swiss-Prot
Match: Q9AE87 (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 9.5e-18
Identity = 63/199 (31.66%), Postives = 88/199 (44.22%), Query Frame = 0

Query: 99  ISLAKSIVGAASSRIWLEPALAALVGYILADLGSGIYHWGIDNYGGASTPIFG-TQIEAF 158
           ++L   + G   +  WL  + A L+GY+ AD  SG  HW  D +G    P+ G   I  F
Sbjct: 42  VALVYRLWGTPYAGTWLLLS-AVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPF 101

Query: 159 QGHHKWPWTITKRQFA-----NNLYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMF 218
           + HH     IT+  F      N L +L  A+    LP+S          F+G     +M 
Sbjct: 102 REHHVDEKAITRHDFVETNGNNCLISLPVAIIALCLPMS-GPGWVFCASFLGAMIFWVMA 161

Query: 219 SQQFHAWAHGKKSEIPPLVVAMQDAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDET 278
           + QFH W+H      P LV  +Q   L++    H  HH  PYN  YCI  G  N+ L   
Sbjct: 162 TNQFHKWSH--MDSPPALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMV 221

Query: 279 KVFEALEMILYFKLGLRPR 292
             F   E ++ +  GL PR
Sbjct: 222 HFFPTAERLITWATGLLPR 236

BLAST of Pay0000710 vs. ExPASy Swiss-Prot
Match: Q5UR78 (Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L630 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-11
Identity = 48/179 (26.82%), Postives = 79/179 (44.13%), Query Frame = 0

Query: 115 LEPALAALVGYILADLGSGIYHWGIDNYGGASTPIFG-TQIEAFQGHHKWPWTITKRQFA 174
           ++  +  ++GY+L+D   GIYHW  D Y    TPI G T I   + HH  P  + +    
Sbjct: 6   IDKFIQIVLGYLLSDFIMGIYHWIKDTYFSPFTPIIGKTFIWGSRLHHVRPRYVLEFTDK 65

Query: 175 NNLYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVA 234
           + +   A+     + P+   F    L  F+      I  +   H + H    E P     
Sbjct: 66  DLIIDSAKWTLSWIGPLFFWFG---LTPFLVTMFIMISLNDVIHKYTHEIDHERPMWATI 125

Query: 235 MQDAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRS 293
           +Q  G   S  +H  HH  P+  NYC V+   N  L++  ++  LE  + +  G++PR+
Sbjct: 126 LQRIGFFQSHDEHHLHHIAPHEINYCPVTPYVNIWLEKINLWRKLESFVEYLTGVKPRA 181

BLAST of Pay0000710 vs. ExPASy TrEMBL
Match: A0A1S3CJN9 (fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.7e-182
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
           SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of Pay0000710 vs. ExPASy TrEMBL
Match: A0A5A7SXQ7 (Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00380 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.1e-181
Identity = 311/313 (99.36%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGV TSSKHGDGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
           SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of Pay0000710 vs. ExPASy TrEMBL
Match: A0A0A0L6B9 (TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G077710 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.0e-173
Identity = 295/313 (94.25%), Postives = 303/313 (96.81%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RAVTFAVLPVSLA DDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of Pay0000710 vs. ExPASy TrEMBL
Match: A0A6J1DD70 (fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019014 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.5e-151
Identity = 260/316 (82.28%), Postives = 279/316 (88.29%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANT----TVNGVGTSSKHGDGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S P PR++VYC+ANT          T  KH DGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQD 240
           YALARAVTF VLP++LAF DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQD
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QH+ HHRPPYNNNYCIVSGIWNQLLDETKVFEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 313
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

BLAST of Pay0000710 vs. ExPASy TrEMBL
Match: A0A6J1IGF1 (fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476701 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.3e-147
Identity = 257/318 (80.82%), Postives = 279/318 (87.74%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSS-----KHGDGQLVIER 60
           MSIL QNKFLLSS++HV+  S   PR+ V CIANTTVN    ++     KHGDGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EVISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWL 120
           E +SP+     NSSTYYSVLNDPSL+STWSHR WV  GCT VLISLAKSI GAASS  WL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQ 240
           LYALARAVTF VLP++LA +DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTE 300
           DAGLLVSR QH+ HHRPPYNNNYCIVSGIWN+LLDETKVFEALEMIL+FKLG+RPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 314
           P+SEWTEETEI+EQTTA+
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

BLAST of Pay0000710 vs. NCBI nr
Match: XP_008463431.1 (PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 648.3 bits (1671), Expect = 3.4e-182
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
           SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of Pay0000710 vs. NCBI nr
Match: KAA0033959.1 (fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desaturase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 645.6 bits (1664), Expect = 2.2e-181
Identity = 311/313 (99.36%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGV TSSKHGDGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
           SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of Pay0000710 vs. NCBI nr
Match: XP_004152938.1 (fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetical protein Csa_011564 [Cucumis sativus])

HSP 1 Score: 616.3 bits (1588), Expect = 1.4e-172
Identity = 295/313 (94.25%), Postives = 303/313 (96.81%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RAVTFAVLPVSLA DDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

BLAST of Pay0000710 vs. NCBI nr
Match: XP_038905900.1 (fatty acid desaturase 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 593.6 bits (1529), Expect = 1.0e-165
Identity = 283/317 (89.27%), Postives = 297/317 (93.69%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVN----GVGTSSKHGDGQLVIERE 60
           MSILAQNKFLLSSRSHVNPCSTP PRRV YCIAN+TVN       T+ KH DGQL+IERE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVFYCIANSTVNVAAGATTTNPKHADGQLLIERE 60

Query: 61  VISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLE 120
           VISP+PLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASS IWLE
Sbjct: 61  VISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           PALAALVGYILADLGSG+YHWGIDNYG  STPIFGTQIEAFQGHHKWPWTITKRQFANN+
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGNVSTPIFGTQIEAFQGHHKWPWTITKRQFANNM 180

Query: 181 YALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQD 240
           YALARAVTFAVLPVSLAFDDP LHGFVGVCSGCIMFSQQFHAWAHGKKSE+PPLVVAMQD
Sbjct: 181 YALARAVTFAVLPVSLAFDDPILHGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQD 240

Query: 241 AGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QH+ HHRPPYNNNYCIVSGIWN++LDETKVFEALEMIL+FKLG+RPRSWTEP
Sbjct: 241 AGLLVSRRQHSAHHRPPYNNNYCIVSGIWNKILDETKVFEALEMILFFKLGVRPRSWTEP 300

Query: 301 NSEWTEETEILEQTTAH 314
           NS WTEETEI+EQTTAH
Sbjct: 301 NSAWTEETEIIEQTTAH 317

BLAST of Pay0000710 vs. NCBI nr
Match: XP_022150986.1 (fatty acid desaturase 4, chloroplastic [Momordica charantia])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 260/316 (82.28%), Postives = 279/316 (88.29%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANT----TVNGVGTSSKHGDGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S P PR++VYC+ANT          T  KH DGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQD 240
           YALARAVTF VLP++LAF DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQD
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QH+ HHRPPYNNNYCIVSGIWNQLLDETKVFEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 313
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

BLAST of Pay0000710 vs. TAIR 10
Match: AT4G27030.1 (fatty acid desaturase A )

HSP 1 Score: 389.8 bits (1000), Expect = 2.1e-108
Identity = 184/292 (63.01%), Postives = 219/292 (75.00%), Query Frame = 0

Query: 23  PTPRRVVYCIANTTVN--------GVGTSSKHGDGQLVIEREVISPSPLTSVNSSTYYSV 82
           P P R    +   TVN           T  +    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALAALVGYILADLGSGI 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   S + LEPALA   GYILADLGSG+
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYG  STP+ GTQIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLLVSRTQHATHHRPPY 262
           +DP  HGFV   + CI+FSQQFHAWAHG KS++PPLVVA+QD GLLVSR QHA HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+KVFEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

BLAST of Pay0000710 vs. TAIR 10
Match: AT1G62190.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 356.7 bits (914), Expect = 1.9e-98
Identity = 170/305 (55.74%), Postives = 217/305 (71.15%), Query Frame = 0

Query: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60
           M++  Q K  L   +++     PT  RV  C   T      T+ +     LV+E+ +++P
Sbjct: 1   MAVSFQTKNPLRPITNIPRSYGPTRVRVT-CSVTT------TNPQLNHENLVVEKRLVNP 60

Query: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120
            PL         S  NDP+L+STW+HR WVA G TT+  S AKSI+G   S +WL+PALA
Sbjct: 61  -PL---------SKNNDPTLQSTWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALA 120

Query: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180
              GY+ ADLGSG+YHW IDNYGGASTPI G Q+EA QGHHK+PWTITKRQFANN Y +A
Sbjct: 121 CYAGYVFADLGSGVYHWAIDNYGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIA 180

Query: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240
           RA+TF VLP++LA ++P  H FV   + CI+ SQQFHAWAHG KS++PPLV+A+QD GLL
Sbjct: 181 RAITFIVLPLNLAINNPLFHSFVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLL 240

Query: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSR  H  HH+ PYN+NYC+VSG WN++LDE+ +F+ALEM L+F+ G+RP SW EPNS+W
Sbjct: 241 VSRKDHPGHHQAPYNSNYCVVSGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDW 288

Query: 301 TEETE 306
           TEETE
Sbjct: 301 TEETE 288

BLAST of Pay0000710 vs. TAIR 10
Match: AT2G22890.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 348.6 bits (893), Expect = 5.2e-96
Identity = 163/291 (56.01%), Postives = 209/291 (71.82%), Query Frame = 0

Query: 11  LSSRSHVNPCSTPTPR--RVVYCIANTTVNGVGTSSKHGDGQLVIEREVISPSPLTSVNS 70
           L ++  +NP +   PR  R  +    +T N    S  + + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN----SQPNHEMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  S +WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSG+YHW  DNYG  STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLLVSRTQHAT 250
           P+ LAFDD  +HGFV + + C++F Q FHAWAHG KS++PPLVV +QD GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WN++LDE+ VF+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ422.9e-10763.01Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 P... [more]
O045842.7e-9755.74Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
O810067.4e-9556.01Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9AE879.5e-1831.66Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1[more]
Q5UR781.7e-1126.82Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=... [more]
Match NameE-valueIdentityDescription
A0A1S3CJN91.7e-182100.00fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 P... [more]
A0A5A7SXQ71.1e-18199.36Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0L6B97.0e-17394.25TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G07... [more]
A0A6J1DD707.5e-15182.28fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1IGF12.3e-14780.82fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114767... [more]
Match NameE-valueIdentityDescription
XP_008463431.13.4e-182100.00PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo][more]
KAA0033959.12.2e-18199.36fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desatu... [more]
XP_004152938.11.4e-17294.25fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetica... [more]
XP_038905900.11.0e-16589.27fatty acid desaturase 4, chloroplastic-like [Benincasa hispida][more]
XP_022150986.11.6e-15082.28fatty acid desaturase 4, chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G27030.12.1e-10863.01fatty acid desaturase A [more]
AT1G62190.11.9e-9855.74Kua-ubiquitin conjugating enzyme hybrid localisation domain [more]
AT2G22890.15.2e-9656.01Kua-ubiquitin conjugating enzyme hybrid localisation domain [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019547B domain of TMEM189, localisation domainPFAMPF10520TMEM189_B_dmaincoord: 125..293
e-value: 7.0E-64
score: 215.1
NoneNo IPR availablePANTHERPTHR48140FAMILY NOT NAMEDcoord: 1..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0000710.1Pay0000710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
cellular_component GO:0016021 integral component of membrane