Pay0000315 (gene) Melon (Payzawat) v1

Overview
NamePay0000315
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptionsubtilisin-like protease SBT1.9
Locationchr03: 29181663 .. 29184991 (-)
RNA-Seq ExpressionPay0000315
SyntenyPay0000315
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACCATCACTCCTCGAAAACAAGTGGAAGAAAGAAAAGAAGAAGAAAACTCTATTTCTAAATCCGAGAAAGACCCCAAAGATTGTTAAAAACTTAAATTATGACAACTTTATTCTACTCTCGAGGGATCTCACAAAAATGAAAGATATTTACCAAAACAATGAGACTAGGAGTATCTTTCACAAAAAAGATATAAAATTTATAAAACTACAGAAGATTCAACAATCCGACCACTTTAAAGAATCAGAATGACAACTTTAATATAACTTGTTGCATATAGGTTTAACCATAGGAAACTAATTTGAGTTAAATATGGTGGGCCCTACTCCAAAGTCCAAAGAAACAAACACTATATATATATATATATATATATATATCTATACCTATATACATACACCTTATCTTGCACAAGGGTTTCCATATTGATCATCAATGGCAGTTCGCCTATACTTTTGGTTTTCTTTGACACCCATTTTCTGGTTGTGCCCCATATTGACAGAGACTCATAATTACATTGTGCACATGAACTCAGCCGCCATGCCTAAGCCTTTTGCTAGCCGCCATAGCTGGTACTCAGCCACCATTTCCTCTGTTCTACATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCTTCCTCCTCTTTCCCATCCAAACTGATCCATACTTACAACCATGCCATTAGTGGTTTCTGCGCAAGTCTCACCCCATCCCAGCTTGAGGCTTTGAAAAATTCCCCTGGCTATCTCTCCTCTGTTCTTGATTCATCAGTTCATGTTGACACAACTCATTCCTCTCACTTCCTTGGTTTAAGCTCCAACCATGGTCTCTTGCCCATCTCGAAATATGGTAGTGATGTTATAATTGGGTTTGTGGATACTGGAATTTGGCCTGACAGTGAGAGCTTTAATGATGATGGGATGTCTGAGATTCCATCTAGATGGAAAGGAGAATGTGAGAGTAGTACTCATTTCAATGTCTCGTTCTGCAACAATAAGCTGATTGGAGCTAGGTTCTTTAACAAAGGACTAATTGCTCAGTTTCCGAATGCAACGATATCTATGAACTCTACACGTGACACGATCGGTCATGGAACTCATACGTCAACCACAGCAGCAGGTACGATTAAAACACTAATCAACTTAGAAACTTAAATTAAGTATATATGTTACAAAATCGATAATAAAGAAGTAATAAAATGAAAACAACGGAGATTTAAATGATTCACTAGTAGTATATGGCTATGTGATATGAAGAGATCAATTTATTATTGGAATGAATATTAGATAAATTATATAGGTTTCTCTAGCTTCAAAGGGTCTGTATATAACACATTCCTTTCAATTTGGTGACGAATTTGTAAATAACGTCAATTTATATACAACATAGTCATTGAGAGCGTTAATATAACATATTCAAGACATTTCAACGAAATACTTTAACACTCTTAAAAATGAAATAAATGCTAAACGTTGCTTTAATACTGTTGAACAAGAAACAAACTTTAAAATTTAAGCTACACTTTTATATGTTTAAATTGTCATTAGACTCAAAAAGTTAAATTAATTAACAGGGAGCTACGTTAAAGAGGCATCATTTTTCGGTTATGGTCGAGGAACTGCAAGAGGTGTGGCTCCAAGAGCACGAGTGGCAATATACAAGGCCATATGGGATGAAGGTAATTCTGTATCAGATGTAGTAGCTGCCATCGATCAAGCAATTTCAGACGGTGTAGATGTGATATCCTTGTCGATCGGCATTGACGGTGTTCCATTGTACAACGATCCAGTTTCTATAGCCACATTCGCTGCTGTCGAGAGAGGTATTTTTGTGGCGACATCCGCCGGAAACAATGGACCTCAACTTGGAACAGTACACAATGGAGCACCTTGGGTTCTGAATGTTGCAGCAGGCACAATGGACCGTGACTTTGGAGGTACAATTACACTTAGCAATGGAGTTTCAGTTTTGGGTTCATCTTTATTTCCTTTAAACACAACCATGGGTTTGTCCCCACTCCCCATTGTTTTCATGGGTGGATGTCAAAACTTGAAGAAACTCAAAAGAATTGGATACAAGATTGTGGTATGTGAAGACAACGATTGGTATTCCTTAACTTCACAAGTTGATAATGTTCAAACTGCAAAAGTTACCTTAGGAATTTTCATTTCCAATATCTTTGATTGGGATAACTTAATCCAAACACCATTCCCTTCTATTTTCGTCAACCCATATCATGGAAACATCATAAAAGATTACATTCATAAAAGCTCTGACCCAAAAGCAGAGGTGAATTTCCACAAGACAATACTTGGGACAAAGCCAGCGCCAACTGTGGCTCGTTACAGCTCAAGAGGGCCATCACAAAGCTGCCCATTTGTATTAAAGCCCGATATTATGGCGCCCGGTGATGCCATTTTAGCTTCATGGCCTCAAAATATACCAGCCATGGATGTAAACTCAACCCCAATTTACACTAAGTTTAATGTAATTTCAGGAACTTCCATGTCTTGCCCACATGCCGCTGGGGTTGCGGCCCTTCTCAAGGGCGCACACCCTCAGTGGAGTCCCGCGGCGATTCGGTCAGCGATGATGACAACGGCCGACGTTGTAGACAACACTCAAACTTCTATCAAAGATATTGGCAACAATAACAAATTTGCTACTCCTTTAGCCATGGGGTCTGGCCATGTTAATCCCAACAAAGCCGTTGATCCGGATTTGATTTACGACGTTGGAATCCAAGACTATGTAAATGTTTTATGTGCATTAAACTATACAGAAAATCAAATCCGAATAATCACTCGATCGGACTCAAACAATTGTGAATACCCGTCGTTGGATTTGAACTACCCTTCTTTTATCATAATTGCGAACTCTAGTGATTCGAAGACAGGAAAAAGAAAAATCTTGAGAGAATTCAAGAGGACATTGACCAAGATTGGAGAAAATAGAGCAACATATGAAGCAAAGTTGAGAGGAATGAAGGGATTCAAAGTGAGAGTGAAGCCAAGGATATTGGAATTCAAAAGAAAGAATCAAAAGTTGAGTTTTGAGCTAAAAATTGCAGGCAGTGCAGAAGAAAGCAACGTCGTTTTTGGTTATCTGAGTTGGGCGGAGGTCGGAGGTAGGCATATCGTTCAAAGTCCAATAGTTGTTGCCGGAATGAGGGTGCAGTGAAACTAACATATATCTGCATGACATGTGATTATTTTCATATAATTCATATTGGGAAGACATTGAATTATGGAATTACTTGTGGAATTAAAAAATTAAACAGAGGGTAAGACAAAGATAAGAT

mRNA sequence

AAACCATCACTCCTCGAAAACAAGTGGAAGAAAGAAAAGAAGAAGAAAACTCTATTTCTAAATCCGAGAAAGACCCCAAAGATTGTTAAAAACTTAAATTATGACAACTTTATTCTACTCTCGAGGGATCTCACAAAAATGAAAGATATTTACCAAAACAATGAGACTAGGAGTATCTTTCACAAAAAAGATATAAAATTTATAAAACTACAGAAGATTCAACAATCCGACCACTTTAAAGAATCAGAATGACAACTTTAATATAACTTGTTGCATATAGGTTTAACCATAGGAAACTAATTTGAGTTAAATATGGTGGGCCCTACTCCAAAGTCCAAAGAAACAAACACTATATATATATATATATATATATATATCTATACCTATATACATACACCTTATCTTGCACAAGGGTTTCCATATTGATCATCAATGGCAGTTCGCCTATACTTTTGGTTTTCTTTGACACCCATTTTCTGGTTGTGCCCCATATTGACAGAGACTCATAATTACATTGTGCACATGAACTCAGCCGCCATGCCTAAGCCTTTTGCTAGCCGCCATAGCTGGTACTCAGCCACCATTTCCTCTGTTCTACATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCTTCCTCCTCTTTCCCATCCAAACTGATCCATACTTACAACCATGCCATTAGTGGTTTCTGCGCAAGTCTCACCCCATCCCAGCTTGAGGCTTTGAAAAATTCCCCTGGCTATCTCTCCTCTGTTCTTGATTCATCAGTTCATGTTGACACAACTCATTCCTCTCACTTCCTTGGTTTAAGCTCCAACCATGGTCTCTTGCCCATCTCGAAATATGGTAGTGATGTTATAATTGGGTTTGTGGATACTGGAATTTGGCCTGACAGTGAGAGCTTTAATGATGATGGGATGTCTGAGATTCCATCTAGATGGAAAGGAGAATGTGAGAGTAGTACTCATTTCAATGTCTCGTTCTGCAACAATAAGCTGATTGGAGCTAGGTTCTTTAACAAAGGACTAATTGCTCAGTTTCCGAATGCAACGATATCTATGAACTCTACACGTGACACGATCGGTCATGGAACTCATACGTCAACCACAGCAGCAGGGAGCTACGTTAAAGAGGCATCATTTTTCGGTTATGGTCGAGGAACTGCAAGAGGTGTGGCTCCAAGAGCACGAGTGGCAATATACAAGGCCATATGGGATGAAGGTAATTCTGTATCAGATGTAGTAGCTGCCATCGATCAAGCAATTTCAGACGGTGTAGATGTGATATCCTTGTCGATCGGCATTGACGGTGTTCCATTGTACAACGATCCAGTTTCTATAGCCACATTCGCTGCTGTCGAGAGAGGTATTTTTGTGGCGACATCCGCCGGAAACAATGGACCTCAACTTGGAACAGTACACAATGGAGCACCTTGGGTTCTGAATGTTGCAGCAGGCACAATGGACCGTGACTTTGGAGGTACAATTACACTTAGCAATGGAGTTTCAGTTTTGGGTTCATCTTTATTTCCTTTAAACACAACCATGGGTTTGTCCCCACTCCCCATTGTTTTCATGGGTGGATGTCAAAACTTGAAGAAACTCAAAAGAATTGGATACAAGATTGTGGTATGTGAAGACAACGATTGGTATTCCTTAACTTCACAAGTTGATAATGTTCAAACTGCAAAAGTTACCTTAGGAATTTTCATTTCCAATATCTTTGATTGGGATAACTTAATCCAAACACCATTCCCTTCTATTTTCGTCAACCCATATCATGGAAACATCATAAAAGATTACATTCATAAAAGCTCTGACCCAAAAGCAGAGGTGAATTTCCACAAGACAATACTTGGGACAAAGCCAGCGCCAACTGTGGCTCGTTACAGCTCAAGAGGGCCATCACAAAGCTGCCCATTTGTATTAAAGCCCGATATTATGGCGCCCGGTGATGCCATTTTAGCTTCATGGCCTCAAAATATACCAGCCATGGATGTAAACTCAACCCCAATTTACACTAAGTTTAATGTAATTTCAGGAACTTCCATGTCTTGCCCACATGCCGCTGGGGTTGCGGCCCTTCTCAAGGGCGCACACCCTCAGTGGAGTCCCGCGGCGATTCGGTCAGCGATGATGACAACGGCCGACGTTGTAGACAACACTCAAACTTCTATCAAAGATATTGGCAACAATAACAAATTTGCTACTCCTTTAGCCATGGGGTCTGGCCATGTTAATCCCAACAAAGCCGTTGATCCGGATTTGATTTACGACGTTGGAATCCAAGACTATGTAAATGTTTTATGTGCATTAAACTATACAGAAAATCAAATCCGAATAATCACTCGATCGGACTCAAACAATTGTGAATACCCGTCGTTGGATTTGAACTACCCTTCTTTTATCATAATTGCGAACTCTAGTGATTCGAAGACAGGAAAAAGAAAAATCTTGAGAGAATTCAAGAGGACATTGACCAAGATTGGAGAAAATAGAGCAACATATGAAGCAAAGTTGAGAGGAATGAAGGGATTCAAAGTGAGAGTGAAGCCAAGGATATTGGAATTCAAAAGAAAGAATCAAAAGTTGAGTTTTGAGCTAAAAATTGCAGGCAGTGCAGAAGAAAGCAACGTCGTTTTTGGTTATCTGAGTTGGGCGGAGGTCGGAGGTAGGCATATCGTTCAAAGTCCAATAGTTGTTGCCGGAATGAGGGTGCAGTGAAACTAACATATATCTGCATGACATGTGATTATTTTCATATAATTCATATTGGGAAGACATTGAATTATGGAATTACTTGTGGAATTAAAAAATTAAACAGAGGGTAAGACAAAGATAAGAT

Coding sequence (CDS)

ATGGCAGTTCGCCTATACTTTTGGTTTTCTTTGACACCCATTTTCTGGTTGTGCCCCATATTGACAGAGACTCATAATTACATTGTGCACATGAACTCAGCCGCCATGCCTAAGCCTTTTGCTAGCCGCCATAGCTGGTACTCAGCCACCATTTCCTCTGTTCTACATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCTTCCTCCTCTTTCCCATCCAAACTGATCCATACTTACAACCATGCCATTAGTGGTTTCTGCGCAAGTCTCACCCCATCCCAGCTTGAGGCTTTGAAAAATTCCCCTGGCTATCTCTCCTCTGTTCTTGATTCATCAGTTCATGTTGACACAACTCATTCCTCTCACTTCCTTGGTTTAAGCTCCAACCATGGTCTCTTGCCCATCTCGAAATATGGTAGTGATGTTATAATTGGGTTTGTGGATACTGGAATTTGGCCTGACAGTGAGAGCTTTAATGATGATGGGATGTCTGAGATTCCATCTAGATGGAAAGGAGAATGTGAGAGTAGTACTCATTTCAATGTCTCGTTCTGCAACAATAAGCTGATTGGAGCTAGGTTCTTTAACAAAGGACTAATTGCTCAGTTTCCGAATGCAACGATATCTATGAACTCTACACGTGACACGATCGGTCATGGAACTCATACGTCAACCACAGCAGCAGGGAGCTACGTTAAAGAGGCATCATTTTTCGGTTATGGTCGAGGAACTGCAAGAGGTGTGGCTCCAAGAGCACGAGTGGCAATATACAAGGCCATATGGGATGAAGGTAATTCTGTATCAGATGTAGTAGCTGCCATCGATCAAGCAATTTCAGACGGTGTAGATGTGATATCCTTGTCGATCGGCATTGACGGTGTTCCATTGTACAACGATCCAGTTTCTATAGCCACATTCGCTGCTGTCGAGAGAGGTATTTTTGTGGCGACATCCGCCGGAAACAATGGACCTCAACTTGGAACAGTACACAATGGAGCACCTTGGGTTCTGAATGTTGCAGCAGGCACAATGGACCGTGACTTTGGAGGTACAATTACACTTAGCAATGGAGTTTCAGTTTTGGGTTCATCTTTATTTCCTTTAAACACAACCATGGGTTTGTCCCCACTCCCCATTGTTTTCATGGGTGGATGTCAAAACTTGAAGAAACTCAAAAGAATTGGATACAAGATTGTGGTATGTGAAGACAACGATTGGTATTCCTTAACTTCACAAGTTGATAATGTTCAAACTGCAAAAGTTACCTTAGGAATTTTCATTTCCAATATCTTTGATTGGGATAACTTAATCCAAACACCATTCCCTTCTATTTTCGTCAACCCATATCATGGAAACATCATAAAAGATTACATTCATAAAAGCTCTGACCCAAAAGCAGAGGTGAATTTCCACAAGACAATACTTGGGACAAAGCCAGCGCCAACTGTGGCTCGTTACAGCTCAAGAGGGCCATCACAAAGCTGCCCATTTGTATTAAAGCCCGATATTATGGCGCCCGGTGATGCCATTTTAGCTTCATGGCCTCAAAATATACCAGCCATGGATGTAAACTCAACCCCAATTTACACTAAGTTTAATGTAATTTCAGGAACTTCCATGTCTTGCCCACATGCCGCTGGGGTTGCGGCCCTTCTCAAGGGCGCACACCCTCAGTGGAGTCCCGCGGCGATTCGGTCAGCGATGATGACAACGGCCGACGTTGTAGACAACACTCAAACTTCTATCAAAGATATTGGCAACAATAACAAATTTGCTACTCCTTTAGCCATGGGGTCTGGCCATGTTAATCCCAACAAAGCCGTTGATCCGGATTTGATTTACGACGTTGGAATCCAAGACTATGTAAATGTTTTATGTGCATTAAACTATACAGAAAATCAAATCCGAATAATCACTCGATCGGACTCAAACAATTGTGAATACCCGTCGTTGGATTTGAACTACCCTTCTTTTATCATAATTGCGAACTCTAGTGATTCGAAGACAGGAAAAAGAAAAATCTTGAGAGAATTCAAGAGGACATTGACCAAGATTGGAGAAAATAGAGCAACATATGAAGCAAAGTTGAGAGGAATGAAGGGATTCAAAGTGAGAGTGAAGCCAAGGATATTGGAATTCAAAAGAAAGAATCAAAAGTTGAGTTTTGAGCTAAAAATTGCAGGCAGTGCAGAAGAAAGCAACGTCGTTTTTGGTTATCTGAGTTGGGCGGAGGTCGGAGGTAGGCATATCGTTCAAAGTCCAATAGTTGTTGCCGGAATGAGGGTGCAGTGA

Protein sequence

MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLTSQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPSLDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ
Homology
BLAST of Pay0000315 vs. ExPASy Swiss-Prot
Match: O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 4.4e-181
Identity = 359/774 (46.38%), Postives = 499/774 (64.47%), Query Frame = 0

Query: 5   LYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSS 64
           L F ++L+   +L   L +   YIVH++ + MP  F   H W+S+TI S+  S  SS   
Sbjct: 7   LLFSWALSAHLFLA--LAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSIKASVPSSVDR 66

Query: 65  SSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVH 124
             S+               KL+++Y++ + GF A L+  +L ALK  PG++S+  D +V 
Sbjct: 67  FHSA--------------PKLVYSYDNVLHGFSAVLSKDELAALKKLPGFISAYKDRTVE 126

Query: 125 VDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGEC 184
             TTH+S FL L+ + GL P S  G DVI+  +D+GIWP+S SF DDGM EIP RWKG C
Sbjct: 127 PHTTHTSDFLKLNPSSGLWPASGLGQDVIVAVLDSGIWPESASFQDDGMPEIPKRWKGIC 186

Query: 185 ESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGSYVKE 244
           +  T FN S CN KLIGA +FNKG++A  P   I+MNS RDT GHGTH ++  AG++ K 
Sbjct: 187 KPGTQFNASMCNRKLIGANYFNKGILANDPTVNITMNSARDTDGHGTHCASITAGNFAKG 246

Query: 245 ASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGV 304
            S FGY  GTARGVAPRAR+A+YK  ++EG   SD++AA+DQA++DGVD+IS+S G   +
Sbjct: 247 VSHFGYAPGTARGVAPRARLAVYKFSFNEGTFTSDLIAAMDQAVADGVDMISISYGYRFI 306

Query: 305 PLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITL 364
           PLY D +SIA+F A+ +G+ V+ SAGN GP +G+++NG+PW+L VA+G  DR F GT+TL
Sbjct: 307 PLYEDAISIASFGAMMKGVLVSASAGNRGPGIGSLNNGSPWILCVASGHTDRTFAGTLTL 366

Query: 365 SNGVSVLGSSLFPLNTTMGLSPLPIVF---MGGCQN---LKKLKRIGYKIVVCEDNDWYS 424
            NG+ + G SLFP    +  S  P+++   +  C +   L +++     IV+C+DN  +S
Sbjct: 367 GNGLKIRGWSLFPARAFVRDS--PVIYNKTLSDCSSEELLSQVENPENTIVICDDNGDFS 426

Query: 425 LTSQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEV 484
              Q+  +  A++   IFIS           P P + VN   G  + +Y+  S  P A +
Sbjct: 427 --DQMRIITRARLKAAIFISEDPGVFRSATFPNPGVVVNKKEGKQVINYVKNSVTPTATI 486

Query: 485 NFHKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPI 544
            F +T L TKPAP VA  S+RGPS+S   + KPDI+APG  ILA++P N+ A  + +  +
Sbjct: 487 TFQETYLDTKPAPVVAASSARGPSRSYLGISKPDILAPGVLILAAYPPNVFATSIGTNIL 546

Query: 545 Y-TKFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGN 604
             T + + SGTSM+ PHAAG+AA+LK AHP+WSP+AIRSAMMTTAD +DNT+  IKD  +
Sbjct: 547 LSTDYILESGTSMAAPHAAGIAAMLKAAHPEWSPSAIRSAMMTTADPLDNTRKPIKD-SD 606

Query: 605 NNKFATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSD-SNNC 664
           NNK ATPL MG+GHV+PN+A+DP L+YD   QDYVN+LC+LN+TE Q + I RS  S+NC
Sbjct: 607 NNKAATPLDMGAGHVDPNRALDPGLVYDATPQDYVNLLCSLNFTEEQFKTIARSSASHNC 666

Query: 665 EYPSLDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRV 724
             PS DLNYPSFI + +   + T    + ++FKRT+T +G+  ATY+AKL+  K   + V
Sbjct: 667 SNPSADLNYPSFIALYSIEGNFT---LLEQKFKRTVTNVGKGAATYKAKLKAPKNSTISV 726

Query: 725 KPRILEFKRKNQKLSFELKI--AGSAEESNVVFGYLSWAEVGGRHIVQSPIVVA 769
            P+IL FK KN+K S+ L I   G   +S  V G ++W E  G H V+SPIV +
Sbjct: 727 SPQILVFKNKNEKQSYTLTIRYIGDEGQSRNV-GSITWVEQNGNHSVRSPIVTS 755

BLAST of Pay0000315 vs. ExPASy Swiss-Prot
Match: Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 2.4e-163
Identity = 328/776 (42.27%), Postives = 469/776 (60.44%), Query Frame = 0

Query: 15  FWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSSSS 74
           F++  +  ET  YI+HM+ +A P PF+   SW+S T++SV+                   
Sbjct: 13  FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI------------------- 72

Query: 75  SSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFL 134
               ++   K+I+ Y  ++ GF A LT S+L+ LK+ PGY+S   D  V + TT S  F+
Sbjct: 73  ----TNRKPKIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFI 132

Query: 135 GLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSF 194
           GL+S  G  P+S YG+ ++IG +DTGIWPDS SF+DDG+  +PS+WKG CE ++    S 
Sbjct: 133 GLNSTSGTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNSS---SL 192

Query: 195 CNNKLIGARFFNKGLIAQFPNATIS----MNSTRDTIGHGTHTSTTAAGSYVKEASFFGY 254
           CN KLIGA+ FNKGL A  P+   +     +S  DTIGHGTH +  AAG++VK AS+F Y
Sbjct: 193 CNKKLIGAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSY 252

Query: 255 GRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGI--------D 314
            +GTA G+AP A +AIYKA W+EG   SDV+AAIDQAI DGV VISLS+G+        D
Sbjct: 253 AQGTASGIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDND 312

Query: 315 GVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI 374
           G  L NDP+++A+FAA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+
Sbjct: 313 GFGLENDPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTL 372

Query: 375 TLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLTSQ 434
           T  N VS    SLFP        P+  +  G  +N    K +  +IVVC +N   ++ S+
Sbjct: 373 TFGNRVSFSFPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVCNEN--INIGSK 432

Query: 435 VDNVQTAKVTLGIFISN-IFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKS-SDPKAEVNF 494
           +  +++      + I++ + +  + I+  FP  F+   H   I+ Y   + ++  A++ F
Sbjct: 433 LHQIRSTGAAAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEF 492

Query: 495 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 554
            KT++GTKPAP V  YSSRGP  S P +LKPDI+APG  IL++WP         + P+++
Sbjct: 493 RKTVIGTKPAPEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFS 552

Query: 555 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 614
            FN+++GTSM+ PH AGVAAL+K  HP WSP+AI+SA+MTTA  +DN             
Sbjct: 553 GFNLLTGTSMAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN------------- 612

Query: 615 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLC-ALNYTENQIRIITRSD-SNNCEY 674
              PLA+G+GHV+ NK ++P LIYD   QD++N LC     +   I IITRS+ S+ C+ 
Sbjct: 613 ---PLAVGAGHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKK 672

Query: 675 PSLDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKP 734
           PS  LNYPS I    S  S        + FKRTLT +GE + +Y  ++RG+KG  V V+P
Sbjct: 673 PSPYLNYPSIIAYFTSDQSSP------KIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEP 732

Query: 735 RILEFKRKNQKLSFELKI-AGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 774
           + L F  KN+KLS+ +++ +    + NVV+G +SW +        S  VVA   VQ
Sbjct: 733 KKLMFSEKNEKLSYTVRLESPRGLQENVVYGLVSWVDEDEAEFEVSCSVVATSLVQ 734

BLAST of Pay0000315 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.2e-141
Identity = 323/808 (39.98%), Postives = 453/808 (56.06%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETH-NYIVHMNSAAMPKPFASRHSWYSATISSVLHSSS 60
           MA   YF+F LT         +     YIVH++  A P  F +   WY+++++S+     
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----- 60

Query: 61  SSSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVL 120
                              +S P  +IHTY+    GF A LT      L + P  +S + 
Sbjct: 61  -------------------TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIP 120

Query: 121 DSSVHVDTTHSSHFLGLSSNH--GLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIP 180
           +   H+ TT S  FLGL S    GLL  S +GSD++IG +DTG+WP+  SF+D G+  +P
Sbjct: 121 EQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVP 180

Query: 181 SRWKGECESSTHFNVSFCNNKLIGARFFNKGLIAQ--FPNATISMNSTRDTIGHGTHTST 240
            +WKG+C +S  F  S CN KL+GARFF  G  A     N T    S RD+ GHGTHT++
Sbjct: 181 IKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTAS 240

Query: 241 TAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVI 300
            +AG YV  AS  GY  G A G+AP+AR+A YK  W+ G   SD++AA D A++DGVDVI
Sbjct: 241 ISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVI 300

Query: 301 SLSIGIDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMD 360
           SLS+G   VP Y D ++I  F A++RGIFV+ SAGN GP   TV N APW+  V AGT+D
Sbjct: 301 SLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTID 360

Query: 361 RDFGGTITLSNGVSVLGSSLFPLNTTMGLSP---LPIVF----MGGCQNLKKL------- 420
           RDF   + L NG  + G S++      GL P    P+V+    +GG      L       
Sbjct: 361 RDFPANVKLGNGKMISGVSVY---GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLD 420

Query: 421 -KRIGYKIVVCEDNDWYSLTSQVDNVQTAKVT--LGIFISN-IFDWDNLIQT--PFPSIF 480
              +  KIV+C+      + S+    +  +    LG+ I+N +FD + L+      P+  
Sbjct: 421 PNLVKGKIVLCD----RGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATS 480

Query: 481 VNPYHGNIIKDYIHKSS------DPKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFVL 540
           V    G+ I+ YI +SS       P A + F  T LG +PAP VA +S+RGP+   P +L
Sbjct: 481 VGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEIL 540

Query: 541 KPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQW 600
           KPD++APG  ILA+WP  I    V S    T+FN++SGTSM+CPH +G+AALLK AHP W
Sbjct: 541 KPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDW 600

Query: 601 SPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGIQ 660
           SPAAIRSA++TTA  VDN+   + D    N  ++ +  GSGHV+P KA+DP L+YD+   
Sbjct: 601 SPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSGHVHPTKAMDPGLVYDITSY 660

Query: 661 DYVNVLCALNYTENQIRIITRSDSNNCEYPSL-----DLNYPSFIIIANSSDSKTGKRKI 720
           DY+N LC  NYT   I  ITR  + +C+         +LNYPSF ++      + G+ K+
Sbjct: 661 DYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNYPSFSVVF----QQYGESKM 720

Query: 721 LREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAG-----S 768
              F RT+T +G++ + YE K+R  +G  V V+P  L F+R  QKLSF +++       S
Sbjct: 721 STHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLS 769

BLAST of Pay0000315 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 3.4e-141
Identity = 302/784 (38.52%), Postives = 438/784 (55.87%), Query Frame = 0

Query: 11  LTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSS 70
           +T   +L    T    YI+ +N +  P+ F + H WY++ ++S                 
Sbjct: 14  ITTFLFLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS----------------- 73

Query: 71  SSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVH-VDTTH 130
                       S L++TY  +  GF A L  ++ ++L +S   +  + +  ++ + TT 
Sbjct: 74  -----------ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTR 133

Query: 131 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 190
           +  FLGL+S  G+  +    + VIIG +DTG+WP+S SF+D  M EIPS+WKGECES + 
Sbjct: 134 TPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSD 193

Query: 191 FNVSFCNNKLIGARFFNKGLIAQFPNATISMN---STRDTIGHGTHTSTTAAGSYVKEAS 250
           F+   CN KLIGAR F+KG          S     S RD  GHGTHTSTTAAGS V+ AS
Sbjct: 194 FDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNAS 253

Query: 251 FFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPL 310
           F GY  GTARG+A RARVA YK  W  G   SD++AA+D+AI DGVDV+SLS+G    P 
Sbjct: 254 FLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPY 313

Query: 311 YNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSN 370
           Y D ++I  F+A+ERG+FV+ SAGN+GP   +V N APWV+ V AGT+DRDF     L N
Sbjct: 314 YRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGN 373

Query: 371 GVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGY--------KIVVCEDNDWYS 430
           G  + G SL+     MG  PL +V+  G  +   L   G         KIVVC+      
Sbjct: 374 GKRLTGVSLYS-GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCD----RG 433

Query: 431 LTSQVDNVQTAKVT--LGIFISN-IFDWDNLIQTP--FPSIFVNPYHGNIIKDYIHKSSD 490
           + ++V+     +    LG+ ++N     + L+      P+I V    G+++++Y+   S 
Sbjct: 434 VNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSK 493

Query: 491 PKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDV 550
           P A + F  T+L  KP+P VA +SSRGP+   P +LKPD++ PG  ILA W   I    +
Sbjct: 494 PTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGL 553

Query: 551 NSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIK 610
           +     T+FN++SGTSMSCPH +G+A LLK AHP+WSP+AI+SA+MTTA V+DNT   + 
Sbjct: 554 DKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLH 613

Query: 611 DIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDS 670
           D  +N+  + P A GSGHV+P KA+ P L+YD+  ++Y+  LC+L+YT + I  I +  S
Sbjct: 614 DAADNS-LSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPS 673

Query: 671 NNCEYPSLD---LNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMK 730
            NC     D   LNYPSF ++        GKR +   + R +T +G   + Y+  + G  
Sbjct: 674 VNCSKKFSDPGQLNYPSFSVLFG------GKRVV--RYTREVTNVGAASSVYKVTVNGAP 733

Query: 731 GFKVRVKPRILEFKRKNQKLSFELKIA---GSAEESNVVFGYLSWAEVGGRHIVQSPIVV 772
              + VKP  L FK   +K  + +      G +  +   FG ++W+    +H V+SP+  
Sbjct: 734 SVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWS--NPQHEVRSPVAF 753

BLAST of Pay0000315 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.0e-137
Identity = 298/764 (39.01%), Postives = 430/764 (56.28%), Query Frame = 0

Query: 27  YIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSSSSSSSSSSFPSKLI 86
           YIVHM  + MP  F    +WY +++ S+  S                         ++L+
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWYDSSLRSISDS-------------------------AELL 91

Query: 87  HTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHG-LLPI 146
           +TY +AI GF   LT  + ++L   PG +S + +    + TT +  FLGL  +   L P 
Sbjct: 92  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPE 151

Query: 147 SKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGARFF 206
           +   SDV++G +DTG+WP+S+S++D+G   IPS WKG CE+ T+F  S CN KLIGARFF
Sbjct: 152 AGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFF 211

Query: 207 NKGLIAQFP--NATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRAR 266
            +G  +     + +    S RD  GHGTHTS+TAAGS V+ AS  GY  GTARG+APRAR
Sbjct: 212 ARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRAR 271

Query: 267 VAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYNDPVSIATFAAVERGI 326
           VA+YK  W  G   SD++AAID+AI+D V+V+S+S+G      Y D V+I  FAA+ERGI
Sbjct: 272 VAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 331

Query: 327 FVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTMG 386
            V+ SAGN GP   ++ N APW+  V AGT+DRDF     L NG +  G SLF     + 
Sbjct: 332 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK-GEALP 391

Query: 387 LSPLPIVFMGGCQNLKK----------LKRIGYKIVVCEDNDWYSLTSQVDNVQTAKVTL 446
              LP ++ G   N              +++  KIV+C+     +   Q  +V  A   +
Sbjct: 392 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRG--INARVQKGDVVKAAGGV 451

Query: 447 GIFISN-IFDWDNLIQTP--FPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPA 506
           G+ ++N   + + L+      P+  V    G+II+ Y+    +P A ++   T++G KP+
Sbjct: 452 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 511

Query: 507 PTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSM 566
           P VA +SSRGP+   P +LKPD++APG  ILA+W        + S     +FN+ISGTSM
Sbjct: 512 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 571

Query: 567 SCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSG 626
           SCPH +G+AALLK  HP+WSPAAIRSA+MTTA         + DI    K +TP   G+G
Sbjct: 572 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAG 631

Query: 627 HVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSD---SNNCEYPSLDLNYPS 686
           HV+P  A +P LIYD+  +DY+  LCALNYT  QIR ++R +     +  Y   DLNYPS
Sbjct: 632 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPS 691

Query: 687 FIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRG-MKGFKVRVKPRILEFKRK 746
           F +  +   +         ++ RT+T +G    TY  K+     G K+ V+P +L FK  
Sbjct: 692 FAVNVDGVGA--------YKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEA 751

Query: 747 NQKLSFEL--KIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVA 769
           N+K S+ +   +  S    +  FG + W++  G+H+V SP+ ++
Sbjct: 752 NEKKSYTVTFTVDSSKPSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of Pay0000315 vs. ExPASy TrEMBL
Match: A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)

HSP 1 Score: 1484.9 bits (3843), Expect = 0.0e+00
Identity = 759/773 (98.19%), Postives = 760/773 (98.32%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
           MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLH    
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLH---- 60

Query: 61  SSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120
                SSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD
Sbjct: 61  -----SSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120

Query: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180
           SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW
Sbjct: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180

Query: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGS 240
           KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTST AAGS
Sbjct: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGS 240

Query: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300
           YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG
Sbjct: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300

Query: 301 IDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360
           IDG+PLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG
Sbjct: 301 IDGIPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360

Query: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLT 420
           TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDND YSLT
Sbjct: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLT 420

Query: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480
           SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF
Sbjct: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480

Query: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540
           HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT
Sbjct: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540

Query: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600
           KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK
Sbjct: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600

Query: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPS 660
           FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCE PS
Sbjct: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPS 660

Query: 661 LDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720
           LDLNYPSFIIIANSSDSKTGKRKIL EFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI
Sbjct: 661 LDLNYPSFIIIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720

Query: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 774
           LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ
Sbjct: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 764

BLAST of Pay0000315 vs. ExPASy TrEMBL
Match: A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)

HSP 1 Score: 1484.9 bits (3843), Expect = 0.0e+00
Identity = 759/773 (98.19%), Postives = 760/773 (98.32%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
           MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLH    
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLH---- 60

Query: 61  SSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120
                SSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD
Sbjct: 61  -----SSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120

Query: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180
           SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW
Sbjct: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180

Query: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGS 240
           KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTST AAGS
Sbjct: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGS 240

Query: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300
           YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG
Sbjct: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300

Query: 301 IDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360
           IDG+PLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG
Sbjct: 301 IDGIPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360

Query: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLT 420
           TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDND YSLT
Sbjct: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLT 420

Query: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480
           SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF
Sbjct: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480

Query: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540
           HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT
Sbjct: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540

Query: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600
           KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK
Sbjct: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600

Query: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPS 660
           FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCE PS
Sbjct: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPS 660

Query: 661 LDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720
           LDLNYPSFIIIANSSDSKTGKRKIL EFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI
Sbjct: 661 LDLNYPSFIIIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720

Query: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 774
           LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ
Sbjct: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 764

BLAST of Pay0000315 vs. ExPASy TrEMBL
Match: A0A0A0LKL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1)

HSP 1 Score: 1390.9 bits (3599), Expect = 0.0e+00
Identity = 703/773 (90.94%), Postives = 726/773 (93.92%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
           MA RLYFWFSL PIFWLCPILTET NYIVHMNSAAMPKPFASRHSWYSATISS+LH    
Sbjct: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLH---- 60

Query: 61  SSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120
                       SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD
Sbjct: 61  ------------SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120

Query: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180
           SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESF DDGMSEIPS+W
Sbjct: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKW 180

Query: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGS 240
           KGECESSTHFNVSFCNNKLIGARFFNKGLI+  P ATIS+NSTRDTIGHGTHTSTTAAGS
Sbjct: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGS 240

Query: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300
           Y+KEASFFGYGRGTARGVAPRARVAIYKAIW+EGNSVSDVVAAIDQAISDGVDVISLSIG
Sbjct: 241 YIKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIG 300

Query: 301 IDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360
           IDGVPLY+DPV+IATFAAVERGIFVATSAGNNGPQL TVHNGAPW+LNVAAGTMDRDFGG
Sbjct: 301 IDGVPLYDDPVAIATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGG 360

Query: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLT 420
           TITLSNGVSVLGSSLFPLN T GLSPLPIVFMGGCQNLKKL+R GYKIVVCED+D YSLT
Sbjct: 361 TITLSNGVSVLGSSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLT 420

Query: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480
           SQVDNVQTA V LGIFISNIFDWDNLIQTPFPSIF+NPYHGNIIKDYIHKSSDPKAEV F
Sbjct: 421 SQVDNVQTANVALGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTF 480

Query: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540
           HKTIL TKPAP VARYSSRGPSQSCPFVLKPDIMAPGD ILASWPQN+PAMDVNSTPIY+
Sbjct: 481 HKTILRTKPAPMVARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYS 540

Query: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600
           KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTAD++DNTQT IKD GNNNK
Sbjct: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNK 600

Query: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPS 660
           FATPLAMGSGHVNPNKA+DPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCE PS
Sbjct: 601 FATPLAMGSGHVNPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPS 660

Query: 661 LDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720
           LDLNYPSFI+I NSSDSKT KRKI  EFKRTLTKIGE+RATYEAKL GMKGFKVRVKP  
Sbjct: 661 LDLNYPSFIMIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNK 720

Query: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 774
           L FKRKNQKLSFELKIAGSA ESN+VFGYLSWAEVGG HI+QSPIVV+GMR+Q
Sbjct: 721 LNFKRKNQKLSFELKIAGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 757

BLAST of Pay0000315 vs. ExPASy TrEMBL
Match: A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 593/769 (77.11%), Postives = 667/769 (86.74%), Query Frame = 0

Query: 3   VRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSS 62
           +R++FW SL  IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SSVL       
Sbjct: 5   MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVL------- 64

Query: 63  SSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSS 122
               +S   +++SSSSSS PSKLIH Y HAI+GF ASLTPSQL+ALKNSPGY+SS+ DSS
Sbjct: 65  --LDTSPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSS 124

Query: 123 VHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKG 182
           V  DTTHSS+FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESFNDDG+S+IPSRWKG
Sbjct: 125 VRADTTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKG 184

Query: 183 ECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGSYV 242
           ECES THFN S CN KLIG RFFNKGLI++FPN TISMNSTRDT GHGTHTSTTAAGSYV
Sbjct: 185 ECESGTHFNASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYV 244

Query: 243 KEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGID 302
           KEASFFGYG+GTARGVAPRARVAIYKAIW EGN+VSDV+AAIDQAISDGVDVISLS+G+D
Sbjct: 245 KEASFFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLD 304

Query: 303 GVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI 362
           GVPLY DPV+IATFAA+ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTI
Sbjct: 305 GVPLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTI 364

Query: 363 TLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLTSQ 422
           TL+NGVSVLGSSLFPLN+ + LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL  Q
Sbjct: 365 TLTNGVSVLGSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQ 424

Query: 423 VDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHK 482
           VDNV++AK+ +G+FISNI DWDNLIQT FPSIF+N YHGN+IKDYI +SS+PKA VNFHK
Sbjct: 425 VDNVESAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHK 484

Query: 483 TILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKF 542
           TI+GTKPAP+VARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQN+ A DV S PIY+KF
Sbjct: 485 TIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKF 544

Query: 543 NVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFA 602
           NV+SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD+VDNTQT IKD+GN NK A
Sbjct: 545 NVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVA 604

Query: 603 TPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPSLD 662
           TPLAMGSGHVNPNKA+DP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE   LD
Sbjct: 605 TPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLD 664

Query: 663 LNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILE 722
           LNYPSFI+  N+SDS+TG+ ++ REFKR +T IGE  ATY AK+  MKG  V V+P  L+
Sbjct: 665 LNYPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLK 724

Query: 723 FKRKNQKLSFELKIAGSA---EESNVVFGYLSWAEVGGRHIVQSPIVVA 769
           FKRKNQ LSF+LKI G      ES+VVFGYL+W EVGG H VQSPIVVA
Sbjct: 725 FKRKNQNLSFKLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA 764

BLAST of Pay0000315 vs. ExPASy TrEMBL
Match: A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 594/772 (76.94%), Postives = 667/772 (86.40%), Query Frame = 0

Query: 3   VRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSS 62
           +R++F  SL  I      LTET NYIVHM+ AAMPKPFA+ HSWYSAT+SSVL       
Sbjct: 5   MRMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVL------- 64

Query: 63  SSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSS 122
                +S   +++SSSSS PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY+SSV DSS
Sbjct: 65  ---LDTSPLRTTTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSS 124

Query: 123 VHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKG 182
           V  DTTHSS+FL L+ N GLLPIS YGSDVIIGFVDTG+WP+SESFND+ +S+IPSRWKG
Sbjct: 125 VRADTTHSSNFLALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKG 184

Query: 183 ECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGSYV 242
           ECES THFN S CN KLIG RFFNKGLIA+FPN TISMNSTRDT GHGTHTSTTAAGSYV
Sbjct: 185 ECESGTHFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYV 244

Query: 243 KEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGID 302
           KEASFFGYG+GTARGVAPRARVAIYKAIW EGN VSDV+AAIDQAISDGVDVISLS+G+D
Sbjct: 245 KEASFFGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLD 304

Query: 303 GVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI 362
           GVPLY DPV+IATFAA+ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTI
Sbjct: 305 GVPLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTI 364

Query: 363 TLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLTSQ 422
           TL+NGVSVLGSSLF LN+ + LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL  Q
Sbjct: 365 TLNNGVSVLGSSLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQ 424

Query: 423 VDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHK 482
           VDNVQ+AK+ +G+FISNI DWDNLIQT FPSIF+N YHGN+IKDYI +SS+PKA VNFHK
Sbjct: 425 VDNVQSAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHK 484

Query: 483 TILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKF 542
           TI+GTKPAP+VARYSSRGPS+SCP VLKPDIMAPGDAILASWPQ + A DV S PIY+KF
Sbjct: 485 TIIGTKPAPSVARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKF 544

Query: 543 NVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFA 602
           NV+SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTADVVDNTQT IKD+GN NK A
Sbjct: 545 NVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVA 604

Query: 603 TPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPSLD 662
           TPLAMGSGHVNPNKA+DP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE PSLD
Sbjct: 605 TPLAMGSGHVNPNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLD 664

Query: 663 LNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILE 722
           LNYPSFII  N+SDS++G+R+I REFKR +T IGE  ATY AK+  MKG  V V+P++L+
Sbjct: 665 LNYPSFIITVNASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLK 724

Query: 723 FKRKNQKLSFELKIAGSA---EESNVVFGYLSWAEVGGRHIVQSPIVVAGMR 772
           FKRKNQ LSF+LKI G      ES+VVFGYL+W EVGG H VQSPIVVAG+R
Sbjct: 725 FKRKNQNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLR 766

BLAST of Pay0000315 vs. NCBI nr
Match: XP_008444560.1 (PREDICTED: subtilisin-like protease SBT1.9 [Cucumis melo] >KAA0060951.1 subtilisin-like protease SBT1.9 [Cucumis melo var. makuwa])

HSP 1 Score: 1484.9 bits (3843), Expect = 0.0e+00
Identity = 759/773 (98.19%), Postives = 760/773 (98.32%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
           MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLH    
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLH---- 60

Query: 61  SSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120
                SSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD
Sbjct: 61  -----SSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120

Query: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180
           SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW
Sbjct: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180

Query: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGS 240
           KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTST AAGS
Sbjct: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGS 240

Query: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300
           YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG
Sbjct: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300

Query: 301 IDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360
           IDG+PLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG
Sbjct: 301 IDGIPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360

Query: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLT 420
           TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDND YSLT
Sbjct: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLT 420

Query: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480
           SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF
Sbjct: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480

Query: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540
           HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT
Sbjct: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540

Query: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600
           KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK
Sbjct: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600

Query: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPS 660
           FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCE PS
Sbjct: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPS 660

Query: 661 LDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720
           LDLNYPSFIIIANSSDSKTGKRKIL EFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI
Sbjct: 661 LDLNYPSFIIIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720

Query: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 774
           LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ
Sbjct: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 764

BLAST of Pay0000315 vs. NCBI nr
Match: XP_004143027.3 (subtilisin-like protease SBT3 [Cucumis sativus] >KGN62450.1 hypothetical protein Csa_018640 [Cucumis sativus])

HSP 1 Score: 1390.9 bits (3599), Expect = 0.0e+00
Identity = 703/773 (90.94%), Postives = 726/773 (93.92%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
           MA RLYFWFSL PIFWLCPILTET NYIVHMNSAAMPKPFASRHSWYSATISS+LH    
Sbjct: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLH---- 60

Query: 61  SSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120
                       SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD
Sbjct: 61  ------------SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120

Query: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180
           SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESF DDGMSEIPS+W
Sbjct: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKW 180

Query: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGS 240
           KGECESSTHFNVSFCNNKLIGARFFNKGLI+  P ATIS+NSTRDTIGHGTHTSTTAAGS
Sbjct: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGS 240

Query: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300
           Y+KEASFFGYGRGTARGVAPRARVAIYKAIW+EGNSVSDVVAAIDQAISDGVDVISLSIG
Sbjct: 241 YIKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIG 300

Query: 301 IDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360
           IDGVPLY+DPV+IATFAAVERGIFVATSAGNNGPQL TVHNGAPW+LNVAAGTMDRDFGG
Sbjct: 301 IDGVPLYDDPVAIATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGG 360

Query: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLT 420
           TITLSNGVSVLGSSLFPLN T GLSPLPIVFMGGCQNLKKL+R GYKIVVCED+D YSLT
Sbjct: 361 TITLSNGVSVLGSSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLT 420

Query: 421 SQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNF 480
           SQVDNVQTA V LGIFISNIFDWDNLIQTPFPSIF+NPYHGNIIKDYIHKSSDPKAEV F
Sbjct: 421 SQVDNVQTANVALGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTF 480

Query: 481 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 540
           HKTIL TKPAP VARYSSRGPSQSCPFVLKPDIMAPGD ILASWPQN+PAMDVNSTPIY+
Sbjct: 481 HKTILRTKPAPMVARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYS 540

Query: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 600
           KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTAD++DNTQT IKD GNNNK
Sbjct: 541 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNK 600

Query: 601 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPS 660
           FATPLAMGSGHVNPNKA+DPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCE PS
Sbjct: 601 FATPLAMGSGHVNPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPS 660

Query: 661 LDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRI 720
           LDLNYPSFI+I NSSDSKT KRKI  EFKRTLTKIGE+RATYEAKL GMKGFKVRVKP  
Sbjct: 661 LDLNYPSFIMIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNK 720

Query: 721 LEFKRKNQKLSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 774
           L FKRKNQKLSFELKIAGSA ESN+VFGYLSWAEVGG HI+QSPIVV+GMR+Q
Sbjct: 721 LNFKRKNQKLSFELKIAGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRLQ 757

BLAST of Pay0000315 vs. NCBI nr
Match: XP_038885776.1 (subtilisin-like protease SBT3 [Benincasa hispida])

HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 647/775 (83.48%), Postives = 702/775 (90.58%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
           MA  LYFWFSL PI WLCPILTET NYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS
Sbjct: 1   MAAGLYFWFSLIPILWLCPILTETDNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 61  SSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLD 120
           SSSS+SSS              SKL H+YNHAI GF ASL+PS+LEALKNSPGY+SSVLD
Sbjct: 61  SSSSASSS--------------SKLTHSYNHAIDGFSASLSPSELEALKNSPGYVSSVLD 120

Query: 121 SSVHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRW 180
           SSV VDTTHSSHFLGLSSN GLLPIS YGSDVIIGFVDTGIWP+ ESFNDDGMSEIPSRW
Sbjct: 121 SSVRVDTTHSSHFLGLSSNFGLLPISNYGSDVIIGFVDTGIWPEGESFNDDGMSEIPSRW 180

Query: 181 KGECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGS 240
           KGECE  THFN S CN KLIGARFFNKGL+A+FPN TISMNSTRDTIGHGTHT+T AAGS
Sbjct: 181 KGECERGTHFNASLCNKKLIGARFFNKGLLAKFPNITISMNSTRDTIGHGTHTATIAAGS 240

Query: 241 YVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIG 300
           YVKEASFFGYGRGTARGVAPRAR+AIYKAIW+EGN+VSDV+AAIDQAISDGVDVISLS+G
Sbjct: 241 YVKEASFFGYGRGTARGVAPRARIAIYKAIWEEGNAVSDVIAAIDQAISDGVDVISLSLG 300

Query: 301 IDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360
           +DGVPLY DPV+IATFAAVERGIFVAT+ GNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG
Sbjct: 301 LDGVPLYQDPVAIATFAAVERGIFVATTTGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGG 360

Query: 361 TITLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDND-WYSL 420
           TITLSNGV++LGSSLFPLN+TM +SPLPIVFMGGCQNLKKLKRIGYKI VC+D D +YSL
Sbjct: 361 TITLSNGVTILGSSLFPLNSTMDVSPLPIVFMGGCQNLKKLKRIGYKIAVCDDKDEYYSL 420

Query: 421 TSQVDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVN 480
           +SQVDNVQ+A V LG+FISNI DWDNLIQT FPSIF++PY+GNIIKDYI KSSDPKAEVN
Sbjct: 421 SSQVDNVQSANVALGVFISNISDWDNLIQTSFPSIFLSPYNGNIIKDYIKKSSDPKAEVN 480

Query: 481 FHKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIY 540
           FHKTILGTKPAP+VARYSSRGPS SCPFVLKPDIMAPGDAILASWPQN+ AM V S PIY
Sbjct: 481 FHKTILGTKPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMYVYSDPIY 540

Query: 541 TKFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNN 600
           TKFN++SGTSMSCPHAAGVAALLK  H  WSPAAIRSAMMTTADVVDN QTSIKDIGN N
Sbjct: 541 TKFNIMSGTSMSCPHAAGVAALLKVVHHGWSPAAIRSAMMTTADVVDNNQTSIKDIGNKN 600

Query: 601 KFATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYP 660
           +FA+PLAMGSGH+NPNKA+DP L+YD+GIQDYVN+LCALNYT++QI+ ITR DSN+CE P
Sbjct: 601 RFASPLAMGSGHINPNKAIDPGLVYDMGIQDYVNLLCALNYTKSQIQTITRLDSNDCENP 660

Query: 661 SLDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPR 720
           S+DLNYPSFIII N+S+SKT K +IL EFKRTLTKIGE RATYEAKLRGMKGFKV V P+
Sbjct: 661 SMDLNYPSFIIIVNTSNSKTSKGEILGEFKRTLTKIGEKRATYEAKLRGMKGFKVGVNPK 720

Query: 721 ILEFKRKNQKLSFELKIAGSA---EESNVVFGYLSWAEVGGRHIVQSPIVVAGMR 772
            LEFKRKNQKLSF+LKIAG+A    E+++VFGYLSW EVGG HIVQSPIVVAG+R
Sbjct: 721 KLEFKRKNQKLSFKLKIAGTARVKRENDMVFGYLSWVEVGGGHIVQSPIVVAGLR 761

BLAST of Pay0000315 vs. NCBI nr
Match: XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1172.9 bits (3033), Expect = 0.0e+00
Identity = 594/771 (77.04%), Postives = 667/771 (86.51%), Query Frame = 0

Query: 3   VRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSS 62
           +R++FW SL  IF     +TET NYIVHM+ AAMPKPFA+ HSWYSAT+SSVL       
Sbjct: 5   MRMFFWISLMVIFLARSTVTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVL------- 64

Query: 63  SSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSS 122
                +S   +++SSSSS PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY+SSV DSS
Sbjct: 65  ---LDTSPLRTTTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSS 124

Query: 123 VHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKG 182
           V  DTTHSS+FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESFNDDG+S+IPSRWKG
Sbjct: 125 VRADTTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKG 184

Query: 183 ECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGSYV 242
           ECES THFN S CN KLIG RFFNKGLIA+FPN TISMNSTRDT GHGTHTSTTAAGSYV
Sbjct: 185 ECESGTHFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYV 244

Query: 243 KEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGID 302
           KEASFFGYG+GTARGVAPRARVAIYKAIW EGN VSDV+AAIDQAISDGVDVI+LS+GID
Sbjct: 245 KEASFFGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVIALSLGID 304

Query: 303 GVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI 362
            VPLY DPV+IATFAA+ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTI
Sbjct: 305 RVPLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTI 364

Query: 363 TLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLTSQ 422
           TL+NGVSVLGSSLFPLN+ + LSPLPI FMG C NLKKLKR+G+KIVVCED+D YSL  Q
Sbjct: 365 TLTNGVSVLGSSLFPLNSAIALSPLPIFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQ 424

Query: 423 VDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHK 482
           VDNVQ+AK+ +G+FISNI DWDNLIQT FPSIF+N YHGN++KDYI++SS+PKA VNFHK
Sbjct: 425 VDNVQSAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHK 484

Query: 483 TILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKF 542
           TI+GTKPAP+VARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQN+ A DV S PIY+KF
Sbjct: 485 TIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKF 544

Query: 543 NVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFA 602
           NV+SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD+VDNTQT IKD+GN NK A
Sbjct: 545 NVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVA 604

Query: 603 TPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPSLD 662
           TPLAMGSGHVNPNKA+DP LIYD+GI+DY N++CALNYT+NQI+ ITRS SN+CE PSLD
Sbjct: 605 TPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLICALNYTKNQIQTITRSTSNDCEKPSLD 664

Query: 663 LNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILE 722
           LNYPSFII  N+SDS+TG+ +I REFKR +T IGE  ATY AK+  MKG  V V+P+ L+
Sbjct: 665 LNYPSFIITVNASDSETGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLK 724

Query: 723 FKRKNQKLSFELKIAGSA---EESNVVFGYLSWAEVGGRHIVQSPIVVAGM 771
           FKRKNQ LSF+LKI G      ES+VVFGYL+W EVGG H VQSPIVV  M
Sbjct: 725 FKRKNQNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVTNM 765

BLAST of Pay0000315 vs. NCBI nr
Match: KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 595/769 (77.37%), Postives = 666/769 (86.61%), Query Frame = 0

Query: 3   VRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSS 62
           +R++FW SL  IF     LT+T NYIVHM+ AAMPKPFA+ HSWYSAT+SSVL  +S   
Sbjct: 5   MRMFFWVSLIVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLR 64

Query: 63  SSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSS 122
           +++SSSSSS          PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY+SSV DSS
Sbjct: 65  TTTSSSSSS----------PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSS 124

Query: 123 VHVDTTHSSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKG 182
           V  DTTHSS+FL LS N GLLPIS YGSDVIIGFVDTG+WP+SESFNDDG+ +IPSRWKG
Sbjct: 125 VRADTTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKG 184

Query: 183 ECESSTHFNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTTAAGSYV 242
           ECES THFN S CN KLIG RFFNKGLIA+FPN TISMNSTRDT GHGTHTSTTAAGSYV
Sbjct: 185 ECESGTHFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYV 244

Query: 243 KEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGID 302
           KEASFFGYG+GTARGVAPRARVAIYKAIW EGN+VSDV+AAIDQAISDGVDVISLS+G+D
Sbjct: 245 KEASFFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLD 304

Query: 303 GVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI 362
           GVPLY DPV+IATFAA+ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTI
Sbjct: 305 GVPLYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTI 364

Query: 363 TLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLTSQ 422
           TL+NGVSVLGSSLFPLN  + LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL  Q
Sbjct: 365 TLTNGVSVLGSSLFPLNLAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQ 424

Query: 423 VDNVQTAKVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHK 482
           VDNV++AK+ +G+FISNI DWDNLIQT FPSIF+N YHGN+IKDYI +SS+PKA VNFHK
Sbjct: 425 VDNVESAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHK 484

Query: 483 TILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKF 542
           TI+GTKPAP+VARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQN+ A DV S PIY+K+
Sbjct: 485 TIIGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKY 544

Query: 543 NVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFA 602
           NV+SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD+VDNTQT IKD+GN NK A
Sbjct: 545 NVLSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVA 604

Query: 603 TPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCEYPSLD 662
           TPLAMGSGHVNPNKA+DP LIYD+GI+DY N+LCALNYT+NQI+ ITRS SN+CE P LD
Sbjct: 605 TPLAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLD 664

Query: 663 LNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILE 722
           LNYPSFII  N+SDS+TG+ +I REFKR +T IGE  ATY AK+  MKG  V V+P  L+
Sbjct: 665 LNYPSFIITVNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLK 724

Query: 723 FKRKNQKLSFELKIAGSA---EESNVVFGYLSWAEVGGRHIVQSPIVVA 769
           FKRKNQ LSF+LKI G      ES+VVFGYL+W EVGG H VQSPIVVA
Sbjct: 725 FKRKNQNLSFKLKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVA 763

BLAST of Pay0000315 vs. TAIR 10
Match: AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 577.4 bits (1487), Expect = 1.7e-164
Identity = 328/776 (42.27%), Postives = 469/776 (60.44%), Query Frame = 0

Query: 15  FWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSSSS 74
           F++  +  ET  YI+HM+ +A P PF+   SW+S T++SV+                   
Sbjct: 13  FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI------------------- 72

Query: 75  SSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFL 134
               ++   K+I+ Y  ++ GF A LT S+L+ LK+ PGY+S   D  V + TT S  F+
Sbjct: 73  ----TNRKPKIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFI 132

Query: 135 GLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSF 194
           GL+S  G  P+S YG+ ++IG +DTGIWPDS SF+DDG+  +PS+WKG CE ++    S 
Sbjct: 133 GLNSTSGTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNSS---SL 192

Query: 195 CNNKLIGARFFNKGLIAQFPNATIS----MNSTRDTIGHGTHTSTTAAGSYVKEASFFGY 254
           CN KLIGA+ FNKGL A  P+   +     +S  DTIGHGTH +  AAG++VK AS+F Y
Sbjct: 193 CNKKLIGAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSY 252

Query: 255 GRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGI--------D 314
            +GTA G+AP A +AIYKA W+EG   SDV+AAIDQAI DGV VISLS+G+        D
Sbjct: 253 AQGTASGIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDND 312

Query: 315 GVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI 374
           G  L NDP+++A+FAA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+
Sbjct: 313 GFGLENDPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTL 372

Query: 375 TLSNGVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDWYSLTSQ 434
           T  N VS    SLFP        P+  +  G  +N    K +  +IVVC +N   ++ S+
Sbjct: 373 TFGNRVSFSFPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVCNEN--INIGSK 432

Query: 435 VDNVQTAKVTLGIFISN-IFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKS-SDPKAEVNF 494
           +  +++      + I++ + +  + I+  FP  F+   H   I+ Y   + ++  A++ F
Sbjct: 433 LHQIRSTGAAAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEF 492

Query: 495 HKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYT 554
            KT++GTKPAP V  YSSRGP  S P +LKPDI+APG  IL++WP         + P+++
Sbjct: 493 RKTVIGTKPAPEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFS 552

Query: 555 KFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNK 614
            FN+++GTSM+ PH AGVAAL+K  HP WSP+AI+SA+MTTA  +DN             
Sbjct: 553 GFNLLTGTSMAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN------------- 612

Query: 615 FATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLC-ALNYTENQIRIITRSD-SNNCEY 674
              PLA+G+GHV+ NK ++P LIYD   QD++N LC     +   I IITRS+ S+ C+ 
Sbjct: 613 ---PLAVGAGHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKK 672

Query: 675 PSLDLNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKP 734
           PS  LNYPS I    S  S        + FKRTLT +GE + +Y  ++RG+KG  V V+P
Sbjct: 673 PSPYLNYPSIIAYFTSDQSSP------KIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEP 732

Query: 735 RILEFKRKNQKLSFELKI-AGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRVQ 774
           + L F  KN+KLS+ +++ +    + NVV+G +SW +        S  VVA   VQ
Sbjct: 733 KKLMFSEKNEKLSYTVRLESPRGLQENVVYGLVSWVDEDEAEFEVSCSVVATSLVQ 734

BLAST of Pay0000315 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 8.3e-143
Identity = 323/808 (39.98%), Postives = 453/808 (56.06%), Query Frame = 0

Query: 1   MAVRLYFWFSLTPIFWLCPILTETH-NYIVHMNSAAMPKPFASRHSWYSATISSVLHSSS 60
           MA   YF+F LT         +     YIVH++  A P  F +   WY+++++S+     
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----- 60

Query: 61  SSSSSSSSSSSSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVL 120
                              +S P  +IHTY+    GF A LT      L + P  +S + 
Sbjct: 61  -------------------TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIP 120

Query: 121 DSSVHVDTTHSSHFLGLSSNH--GLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIP 180
           +   H+ TT S  FLGL S    GLL  S +GSD++IG +DTG+WP+  SF+D G+  +P
Sbjct: 121 EQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVP 180

Query: 181 SRWKGECESSTHFNVSFCNNKLIGARFFNKGLIAQ--FPNATISMNSTRDTIGHGTHTST 240
            +WKG+C +S  F  S CN KL+GARFF  G  A     N T    S RD+ GHGTHT++
Sbjct: 181 IKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTAS 240

Query: 241 TAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVI 300
            +AG YV  AS  GY  G A G+AP+AR+A YK  W+ G   SD++AA D A++DGVDVI
Sbjct: 241 ISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVI 300

Query: 301 SLSIGIDGVPLYNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMD 360
           SLS+G   VP Y D ++I  F A++RGIFV+ SAGN GP   TV N APW+  V AGT+D
Sbjct: 301 SLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTID 360

Query: 361 RDFGGTITLSNGVSVLGSSLFPLNTTMGLSP---LPIVF----MGGCQNLKKL------- 420
           RDF   + L NG  + G S++      GL P    P+V+    +GG      L       
Sbjct: 361 RDFPANVKLGNGKMISGVSVY---GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLD 420

Query: 421 -KRIGYKIVVCEDNDWYSLTSQVDNVQTAKVT--LGIFISN-IFDWDNLIQT--PFPSIF 480
              +  KIV+C+      + S+    +  +    LG+ I+N +FD + L+      P+  
Sbjct: 421 PNLVKGKIVLCD----RGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATS 480

Query: 481 VNPYHGNIIKDYIHKSS------DPKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFVL 540
           V    G+ I+ YI +SS       P A + F  T LG +PAP VA +S+RGP+   P +L
Sbjct: 481 VGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEIL 540

Query: 541 KPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQW 600
           KPD++APG  ILA+WP  I    V S    T+FN++SGTSM+CPH +G+AALLK AHP W
Sbjct: 541 KPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDW 600

Query: 601 SPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGIQ 660
           SPAAIRSA++TTA  VDN+   + D    N  ++ +  GSGHV+P KA+DP L+YD+   
Sbjct: 601 SPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSGHVHPTKAMDPGLVYDITSY 660

Query: 661 DYVNVLCALNYTENQIRIITRSDSNNCEYPSL-----DLNYPSFIIIANSSDSKTGKRKI 720
           DY+N LC  NYT   I  ITR  + +C+         +LNYPSF ++      + G+ K+
Sbjct: 661 DYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNYPSFSVVF----QQYGESKM 720

Query: 721 LREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAG-----S 768
              F RT+T +G++ + YE K+R  +G  V V+P  L F+R  QKLSF +++       S
Sbjct: 721 STHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLS 769

BLAST of Pay0000315 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 2.4e-142
Identity = 302/784 (38.52%), Postives = 438/784 (55.87%), Query Frame = 0

Query: 11  LTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSS 70
           +T   +L    T    YI+ +N +  P+ F + H WY++ ++S                 
Sbjct: 14  ITTFLFLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS----------------- 73

Query: 71  SSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVH-VDTTH 130
                       S L++TY  +  GF A L  ++ ++L +S   +  + +  ++ + TT 
Sbjct: 74  -----------ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTR 133

Query: 131 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 190
           +  FLGL+S  G+  +    + VIIG +DTG+WP+S SF+D  M EIPS+WKGECES + 
Sbjct: 134 TPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSD 193

Query: 191 FNVSFCNNKLIGARFFNKGLIAQFPNATISMN---STRDTIGHGTHTSTTAAGSYVKEAS 250
           F+   CN KLIGAR F+KG          S     S RD  GHGTHTSTTAAGS V+ AS
Sbjct: 194 FDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNAS 253

Query: 251 FFGYGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPL 310
           F GY  GTARG+A RARVA YK  W  G   SD++AA+D+AI DGVDV+SLS+G    P 
Sbjct: 254 FLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPY 313

Query: 311 YNDPVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSN 370
           Y D ++I  F+A+ERG+FV+ SAGN+GP   +V N APWV+ V AGT+DRDF     L N
Sbjct: 314 YRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGN 373

Query: 371 GVSVLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGY--------KIVVCEDNDWYS 430
           G  + G SL+     MG  PL +V+  G  +   L   G         KIVVC+      
Sbjct: 374 GKRLTGVSLYS-GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCD----RG 433

Query: 431 LTSQVDNVQTAKVT--LGIFISN-IFDWDNLIQTP--FPSIFVNPYHGNIIKDYIHKSSD 490
           + ++V+     +    LG+ ++N     + L+      P+I V    G+++++Y+   S 
Sbjct: 434 VNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSK 493

Query: 491 PKAEVNFHKTILGTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDV 550
           P A + F  T+L  KP+P VA +SSRGP+   P +LKPD++ PG  ILA W   I    +
Sbjct: 494 PTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGL 553

Query: 551 NSTPIYTKFNVISGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIK 610
           +     T+FN++SGTSMSCPH +G+A LLK AHP+WSP+AI+SA+MTTA V+DNT   + 
Sbjct: 554 DKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLH 613

Query: 611 DIGNNNKFATPLAMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDS 670
           D  +N+  + P A GSGHV+P KA+ P L+YD+  ++Y+  LC+L+YT + I  I +  S
Sbjct: 614 DAADNS-LSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPS 673

Query: 671 NNCEYPSLD---LNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMK 730
            NC     D   LNYPSF ++        GKR +   + R +T +G   + Y+  + G  
Sbjct: 674 VNCSKKFSDPGQLNYPSFSVLFG------GKRVV--RYTREVTNVGAASSVYKVTVNGAP 733

Query: 731 GFKVRVKPRILEFKRKNQKLSFELKIA---GSAEESNVVFGYLSWAEVGGRHIVQSPIVV 772
              + VKP  L FK   +K  + +      G +  +   FG ++W+    +H V+SP+  
Sbjct: 734 SVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWS--NPQHEVRSPVAF 753

BLAST of Pay0000315 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 492.3 bits (1266), Expect = 7.3e-139
Identity = 298/764 (39.01%), Postives = 430/764 (56.28%), Query Frame = 0

Query: 27  YIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSSSSSSSSSSFPSKLI 86
           YIVHM  + MP  F    +WY +++ S+  S                         ++L+
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWYDSSLRSISDS-------------------------AELL 91

Query: 87  HTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHG-LLPI 146
           +TY +AI GF   LT  + ++L   PG +S + +    + TT +  FLGL  +   L P 
Sbjct: 92  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPE 151

Query: 147 SKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGARFF 206
           +   SDV++G +DTG+WP+S+S++D+G   IPS WKG CE+ T+F  S CN KLIGARFF
Sbjct: 152 AGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFF 211

Query: 207 NKGLIAQFP--NATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRAR 266
            +G  +     + +    S RD  GHGTHTS+TAAGS V+ AS  GY  GTARG+APRAR
Sbjct: 212 ARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRAR 271

Query: 267 VAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYNDPVSIATFAAVERGI 326
           VA+YK  W  G   SD++AAID+AI+D V+V+S+S+G      Y D V+I  FAA+ERGI
Sbjct: 272 VAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 331

Query: 327 FVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTMG 386
            V+ SAGN GP   ++ N APW+  V AGT+DRDF     L NG +  G SLF     + 
Sbjct: 332 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK-GEALP 391

Query: 387 LSPLPIVFMGGCQNLKK----------LKRIGYKIVVCEDNDWYSLTSQVDNVQTAKVTL 446
              LP ++ G   N              +++  KIV+C+     +   Q  +V  A   +
Sbjct: 392 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRG--INARVQKGDVVKAAGGV 451

Query: 447 GIFISN-IFDWDNLIQTP--FPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPA 506
           G+ ++N   + + L+      P+  V    G+II+ Y+    +P A ++   T++G KP+
Sbjct: 452 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 511

Query: 507 PTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSM 566
           P VA +SSRGP+   P +LKPD++APG  ILA+W        + S     +FN+ISGTSM
Sbjct: 512 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 571

Query: 567 SCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSG 626
           SCPH +G+AALLK  HP+WSPAAIRSA+MTTA         + DI    K +TP   G+G
Sbjct: 572 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAG 631

Query: 627 HVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSD---SNNCEYPSLDLNYPS 686
           HV+P  A +P LIYD+  +DY+  LCALNYT  QIR ++R +     +  Y   DLNYPS
Sbjct: 632 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPS 691

Query: 687 FIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRG-MKGFKVRVKPRILEFKRK 746
           F +  +   +         ++ RT+T +G    TY  K+     G K+ V+P +L FK  
Sbjct: 692 FAVNVDGVGA--------YKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEA 751

Query: 747 NQKLSFEL--KIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVA 769
           N+K S+ +   +  S    +  FG + W++  G+H+V SP+ ++
Sbjct: 752 NEKKSYTVTFTVDSSKPSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of Pay0000315 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 488.8 bits (1257), Expect = 8.0e-138
Identity = 301/773 (38.94%), Postives = 427/773 (55.24%), Query Frame = 0

Query: 27  YIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSSSSSSSSSSFPSKLI 86
           +I  ++  +MP  F + + WYS   +                              S+++
Sbjct: 28  FIFRIDGGSMPSIFPTHYHWYSTEFAE----------------------------ESRIV 87

Query: 87  HTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGLLPIS 146
           H Y+    GF A +TP + + L+N P  L+   D    + TT S  FLGL +  GL   S
Sbjct: 88  HVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSES 147

Query: 147 KYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGARFFN 206
            YGSDVIIG  DTGIWP+  SF+D  +  IP RW+G CES   F+   CN K+IGARFF 
Sbjct: 148 DYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFA 207

Query: 207 KGLIAQF---PNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRAR 266
           KG  A      N T+   S RD  GHGTHTS+TAAG +  +AS  GY  G A+GVAP+AR
Sbjct: 208 KGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKAR 267

Query: 267 VAIYKAIW-DEGNSVSDVVAAIDQAISDGVDVISLSI-GIDGV--PLYNDPVSIATFAAV 326
           +A YK  W D G   SD++AA D A+ DGVDVIS+SI G DG+  P Y DP++I ++ A 
Sbjct: 268 IAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAA 327

Query: 327 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLF--- 386
            +GIFV++SAGN GP   +V N APWV  V A T+DR+F     L +G  + G SL+   
Sbjct: 328 SKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGV 387

Query: 387 PLNTTMGLSPLPIVFMGG---------CQNLKKLKRIGYKIVVCEDNDWYSLTSQVDNVQ 446
           PLN  M     P+V+ G           +N    K++  KIV+C+      +   +   +
Sbjct: 388 PLNGRM----FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKK 447

Query: 447 TAKVTLGIFISNIFDWDNLIQTP--FPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTIL 506
              V + I  +   + + L+      P+  V    G+ IK Y     +P A ++F  TI+
Sbjct: 448 AGGVGM-ILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 507

Query: 507 GTKPAPTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVI 566
           G KPAP +A +S RGP+   P +LKPD++APG  ILA+W   +    + S P  T+FN++
Sbjct: 508 GIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 567

Query: 567 SGTSMSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPL 626
           SGTSM+CPH +G AALLK AHP WSPA IRSAMMTT ++VDN+  S+ D  +  K ATP 
Sbjct: 568 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPY 627

Query: 627 AMGSGHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSN--NCEYPSL-D 686
             GSGH+N  +A++P L+YD+   DY+  LC++ Y    I++ITR+         PS  +
Sbjct: 628 DYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGN 687

Query: 687 LNYPSFIIIANSSDSKTGKRKILREFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILE 746
           LNYPS   +  ++      + ++    RT T +G+  A Y A++   +G  V VKP  L 
Sbjct: 688 LNYPSITAVFPTNRRGLVSKTVI----RTATNVGQAEAVYRARIESPRGVTVTVKPPRLV 747

Query: 747 FKRKNQKLSFELKIAGSAE-----ESNVVFGYLSWAEVGGRHIVQSPIVVAGM 771
           F    ++ S+ + +  +       E+  VFG ++W + GG+H+V+SPIVV  M
Sbjct: 748 FTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O827774.4e-18146.38Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1[more]
Q9FHA42.4e-16342.27Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 S... [more]
Q9LUM31.2e-14139.98Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Q9ZUF63.4e-14138.52Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
O653511.0e-13739.01Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7V5890.0e+0098.19Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BBC30.0e+0098.19subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=... [more]
A0A0A0LKL60.0e+0090.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1[more]
A0A6J1HH070.0e+0077.11subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE... [more]
A0A6J1K9W20.0e+0076.94subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3... [more]
Match NameE-valueIdentityDescription
XP_008444560.10.0e+0098.19PREDICTED: subtilisin-like protease SBT1.9 [Cucumis melo] >KAA0060951.1 subtilis... [more]
XP_004143027.30.0e+0090.94subtilisin-like protease SBT3 [Cucumis sativus] >KGN62450.1 hypothetical protein... [more]
XP_038885776.10.0e+0083.48subtilisin-like protease SBT3 [Benincasa hispida][more]
XP_023546329.10.0e+0077.04subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo][more]
KAG6598336.10.0e+0077.37Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G67090.11.7e-16442.27Subtilisin-like serine endopeptidase family protein [more]
AT3G14240.18.3e-14339.98Subtilase family protein [more]
AT2G05920.12.4e-14238.52Subtilase family protein [more]
AT5G67360.17.3e-13939.01Subtilase family protein [more]
AT4G34980.18.0e-13838.94subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 149..168
score: 31.11
coord: 225..238
score: 42.67
coord: 546..562
score: 62.08
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 662..767
e-value: 6.9E-25
score: 87.1
NoneNo IPR availableGENE3D2.60.40.2310coord: 635..770
e-value: 1.1E-35
score: 124.2
NoneNo IPR availableGENE3D3.50.30.30coord: 355..485
e-value: 2.2E-161
score: 539.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..79
NoneNo IPR availablePANTHERPTHR10795:SF669PUTATIVE-RELATEDcoord: 17..770
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 130..618
score: 27.464661
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 361..476
e-value: 2.82279E-10
score: 56.6531
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 27..125
e-value: 4.3E-12
score: 46.5
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 21..125
e-value: 1.7E-14
score: 56.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 151..631
e-value: 2.2E-161
score: 539.7
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 126..620
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 149..588
e-value: 5.0E-45
score: 153.9
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 17..770
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 547..557
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 123..583
e-value: 4.96372E-135
score: 400.05

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0000315.1Pay0000315.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity