Pay0000233 (gene) Melon (Payzawat) v1

Overview
NamePay0000233
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionPectate lyase
Locationchr03: 23397533 .. 23399332 (+)
RNA-Seq ExpressionPay0000233
SyntenyPay0000233
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGAAAGAAAGAAAGAAAGAAAAACAACATACAATGGCTGGCTTTAGTACCTTGTTTGTGTGGTCGTTGTTGTTTTTTGTGGTAGCAAACGCCAATGTAGATGAAGATGAATACTGGAATACTCGTGCCACCGAAGCTAAGAAAGTTGCAAAATCTGCCTTCAATCCTAACCCTGAAATCGTTACCGATAACTTGAACGCCGCCGTTAGTCGGTGAGTTTTTTTTTTTTTTTTCTTTTAATGAAAATGAAATGTTTGGCTCTGATGTATCATGTATATCATGGTTGGTTTGTTTTGGTAGAGCGATGGTGGGTCGCAACAGTACGAGAAGGAGCCTGAAGAAGTATAGAGGGCCATGCTTGGCGACGAACCCCATTGATAGATGTTGGAGATGCGATCGAAATTGGGCTAAAAACCGAAAGAAGCTAGCGGAATGTCCATTGGGGTTCGGCCACCAAACCATTGGTGGAAAGAGAGGACCAATCTACGTGGTGACAGACGCGTCTGACGATGACTTAGTTAACCCTAAACCTGGAACCCTACGATATGGTGTGATCCAAAAAGGACCACTTTGGATCATCTTCGGGAGAAGCATGATCATTAGGCTGAGTCAAGAGCTAATGATCTCAAGCGACAAGACCATCGACGCTCGTGGCGCTAACGTCCACATTGCCTTTGGCGCTGGTCTCACCATTCAATTCGTCAACAATGTAATCATTCATAACCTTCACATCCATGACATTATATCCAAATCGGGTGGAATGATTAGAGATTCAGTGGATCATTATGGGTTTAGAACACAAAGCGATGGCGATGGCATCTCCATCTTTGGTTCTTCTCATGTTTGGATCGACCACGTTTCCATGTCCAATTGTGAAGATGGTCTTATTGACGCCATCATGGGTTCCACAGCAATCACTATCTCCAATTGTCATTTCACTCACCACAACGAGGTAATTAACAACAACAAACACTTTTTTTTTTTCATGATCATATATTATATAGACACGTATGTCCACACACATGTATGCATGCATACAGGTGATGTTATTTGGAGCGAGCGATAGCTACGCAGAAGACGAAATTATGCAAGTAACCCTAGCATTCAACCACTTTGGGAGAGGATTGGTGCAAAGGATGCCAAGGTGTAGATGGGGTTTCTTCCATGTTGTTAACAATGACTACACTCATTGGCTTATGTACGCGATCGGCGGTAGCCAACATCCTACCATTGTTAGCCAAGGCAATCGTTTCATCGCTCCTCCAAACCCTAACGCCAAACAGGTAATATAATTACAACCATAAAACGGAAAGCCCAAAAAACACTACTATAAGTATTAGCAATATGACGGATTTGAGTTTGTATATATTGGTAGGTAACAAAGAGAGAATATGCAATGGAAGACGAATGGAAGCATTGGTCATGGAGATCAGAAGGAGATGAATTGATGAATGGAGCAGTGTTTATTCCATCAGGTGACCAAACAAAAGGGAAGAAGAAAGGCTTATCAAGATATGATATGATTTCCTTCAAGCCAGGAACCTACGTCAGGAGACTTGCACGCTTTTCTGGCACACTTGAATGCACCCCAGGCAAACCATGCTAGCAAATTAAAATTTCTTTTTTTCTTTCCTTTTTTTTTTTTTTTTTGTTCTTTTCTAAATGTTGTTATATTTTCCTTTAGATATAAATAATTTCGGAATTTTGAAAAGGGTATTAGTCTTCTCCATTATCGTCGCTCAAATCTGTGCAGGGAAGAAGGAAGAAGAGACCAAAAAAAAAAAAAAGAAAAGAAAAA

mRNA sequence

AAAAGAAAGAAAGAAAGAAAGAAAAACAACATACAATGGCTGGCTTTAGTACCTTGTTTGTGTGGTCGTTGTTGTTTTTTGTGGTAGCAAACGCCAATGTAGATGAAGATGAATACTGGAATACTCGTGCCACCGAAGCTAAGAAAGTTGCAAAATCTGCCTTCAATCCTAACCCTGAAATCGTTACCGATAACTTGAACGCCGCCGTTAGTCGAGCGATGGTGGGTCGCAACAGTACGAGAAGGAGCCTGAAGAAGTATAGAGGGCCATGCTTGGCGACGAACCCCATTGATAGATGTTGGAGATGCGATCGAAATTGGGCTAAAAACCGAAAGAAGCTAGCGGAATGTCCATTGGGGTTCGGCCACCAAACCATTGGTGGAAAGAGAGGACCAATCTACGTGGTGACAGACGCGTCTGACGATGACTTAGTTAACCCTAAACCTGGAACCCTACGATATGGTGTGATCCAAAAAGGACCACTTTGGATCATCTTCGGGAGAAGCATGATCATTAGGCTGAGTCAAGAGCTAATGATCTCAAGCGACAAGACCATCGACGCTCGTGGCGCTAACGTCCACATTGCCTTTGGCGCTGGTCTCACCATTCAATTCGTCAACAATGTAATCATTCATAACCTTCACATCCATGACATTATATCCAAATCGGGTGGAATGATTAGAGATTCAGTGGATCATTATGGGTTTAGAACACAAAGCGATGGCGATGGCATCTCCATCTTTGGTTCTTCTCATGTTTGGATCGACCACGTTTCCATGTCCAATTGTGAAGATGGTCTTATTGACGCCATCATGGGTTCCACAGCAATCACTATCTCCAATTGTCATTTCACTCACCACAACGAGGTGATGTTATTTGGAGCGAGCGATAGCTACGCAGAAGACGAAATTATGCAAGTAACCCTAGCATTCAACCACTTTGGGAGAGGATTGGTGCAAAGGATGCCAAGGTGTAGATGGGGTTTCTTCCATGTTGTTAACAATGACTACACTCATTGGCTTATGTACGCGATCGGCGGTAGCCAACATCCTACCATTGTTAGCCAAGGCAATCGTTTCATCGCTCCTCCAAACCCTAACGCCAAACAGGTAACAAAGAGAGAATATGCAATGGAAGACGAATGGAAGCATTGGTCATGGAGATCAGAAGGAGATGAATTGATGAATGGAGCAGTGTTTATTCCATCAGGTGACCAAACAAAAGGGAAGAAGAAAGGCTTATCAAGATATGATATGATTTCCTTCAAGCCAGGAACCTACGTCAGGAGACTTGCACGCTTTTCTGGCACACTTGAATGCACCCCAGGCAAACCATGCTAGCAAATTAAAATTTCTTTTTTTCTTTCCTTTTTTTTTTTTTTTTTGTTCTTTTCTAAATGTTGTTATATTTTCCTTTAGATATAAATAATTTCGGAATTTTGAAAAGGGTATTAGTCTTCTCCATTATCGTCGCTCAAATCTGTGCAGGGAAGAAGGAAGAAGAGACCAAAAAAAAAAAAAAGAAAAGAAAAA

Coding sequence (CDS)

ATGGCTGGCTTTAGTACCTTGTTTGTGTGGTCGTTGTTGTTTTTTGTGGTAGCAAACGCCAATGTAGATGAAGATGAATACTGGAATACTCGTGCCACCGAAGCTAAGAAAGTTGCAAAATCTGCCTTCAATCCTAACCCTGAAATCGTTACCGATAACTTGAACGCCGCCGTTAGTCGAGCGATGGTGGGTCGCAACAGTACGAGAAGGAGCCTGAAGAAGTATAGAGGGCCATGCTTGGCGACGAACCCCATTGATAGATGTTGGAGATGCGATCGAAATTGGGCTAAAAACCGAAAGAAGCTAGCGGAATGTCCATTGGGGTTCGGCCACCAAACCATTGGTGGAAAGAGAGGACCAATCTACGTGGTGACAGACGCGTCTGACGATGACTTAGTTAACCCTAAACCTGGAACCCTACGATATGGTGTGATCCAAAAAGGACCACTTTGGATCATCTTCGGGAGAAGCATGATCATTAGGCTGAGTCAAGAGCTAATGATCTCAAGCGACAAGACCATCGACGCTCGTGGCGCTAACGTCCACATTGCCTTTGGCGCTGGTCTCACCATTCAATTCGTCAACAATGTAATCATTCATAACCTTCACATCCATGACATTATATCCAAATCGGGTGGAATGATTAGAGATTCAGTGGATCATTATGGGTTTAGAACACAAAGCGATGGCGATGGCATCTCCATCTTTGGTTCTTCTCATGTTTGGATCGACCACGTTTCCATGTCCAATTGTGAAGATGGTCTTATTGACGCCATCATGGGTTCCACAGCAATCACTATCTCCAATTGTCATTTCACTCACCACAACGAGGTGATGTTATTTGGAGCGAGCGATAGCTACGCAGAAGACGAAATTATGCAAGTAACCCTAGCATTCAACCACTTTGGGAGAGGATTGGTGCAAAGGATGCCAAGGTGTAGATGGGGTTTCTTCCATGTTGTTAACAATGACTACACTCATTGGCTTATGTACGCGATCGGCGGTAGCCAACATCCTACCATTGTTAGCCAAGGCAATCGTTTCATCGCTCCTCCAAACCCTAACGCCAAACAGGTAACAAAGAGAGAATATGCAATGGAAGACGAATGGAAGCATTGGTCATGGAGATCAGAAGGAGATGAATTGATGAATGGAGCAGTGTTTATTCCATCAGGTGACCAAACAAAAGGGAAGAAGAAAGGCTTATCAAGATATGATATGATTTCCTTCAAGCCAGGAACCTACGTCAGGAGACTTGCACGCTTTTCTGGCACACTTGAATGCACCCCAGGCAAACCATGCTAG

Protein sequence

MAGFSTLFVWSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLARFSGTLECTPGKPC
Homology
BLAST of Pay0000233 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.8e-160
Identity = 273/432 (63.19%), Postives = 334/432 (77.31%), Query Frame = 0

Query: 8   FVWSLLFF---VVANANVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSRAMV 67
           F++SLLFF    +++AN+ E DEYW  ++  A+  AK A+ P+PE VT++ N AV  +  
Sbjct: 7   FLYSLLFFASAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFE 66

Query: 68  GRNSTRRSLKKYR-GPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPIY 127
           G NSTRR+L+  + G CLATNPIDRCWRC +NW+ NRK L +C  GFG +T GG  G IY
Sbjct: 67  G-NSTRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIY 126

Query: 128 VVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANVH 187
           VVTD SDD L +PK GTLR+GVIQ  PLWIIFG+SM+IRL QEL+I++DKTID RGANV 
Sbjct: 127 VVTDPSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQ 186

Query: 188 IAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVW 247
           IA GA LT+QFV+NVIIH +HIHDI    GG+IRDS  H G RT+SDGDGISI GSS++W
Sbjct: 187 IAGGAQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIW 246

Query: 248 IDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNHF 307
           IDHVS++ C DGLID I+GSTAITISNCH T H++VML GASD+Y +DEIMQVT+AFNHF
Sbjct: 247 IDHVSLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHF 306

Query: 308 GRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTKR 367
           GRGLVQRMPRCR+GF HVVNNDYTHW+MYA+GGSQHPTI+SQGNR+IAP    AK+VTKR
Sbjct: 307 GRGLVQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKR 366

Query: 368 EYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLARF 427
           +YA   EW  W+W+S+GD  ++GA F+ SG   + K    S+ D+I  KPGT+V+RL RF
Sbjct: 367 DYAEPAEWSKWTWKSQGDLFVSGAFFVESGGPFENK---YSKKDLIKAKPGTFVQRLTRF 426

Query: 428 SGTLECTPGKPC 435
           SG L C     C
Sbjct: 427 SGALNCKENMEC 434

BLAST of Pay0000233 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 4.2e-157
Identity = 270/421 (64.13%), Postives = 313/421 (74.35%), Query Frame = 0

Query: 25  DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSRAM--------VGRNSTRRSL--KK 84
           DE W  RA EAK+ A++ + P+PE VT   N  +   M           NSTRR L  KK
Sbjct: 32  DEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKELKKVKGTHNNSTRRGLGTKK 91

Query: 85  YRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDDDLVN 144
           Y GPC+ TNPID+CWRCD NWA NRKKLA+C +GFG + IGGK G  YVVTD S DD  +
Sbjct: 92  YTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIGGKDGEFYVVTDNS-DDYND 151

Query: 145 PKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANVHIAFGAGLTIQFV 204
           PKPGTLR+ VIQK PLWIIF R M IRL QE+++ SDKTIDARG NVHI  GAG+T+Q++
Sbjct: 152 PKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTIDARGVNVHITKGAGITLQYI 211

Query: 205 NNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCEDG 264
            NVIIH LHIHDI+  +GGM+RD+VDH G RT+SDGDGISIFG+S++WIDHVSM  C DG
Sbjct: 212 KNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISIFGASYIWIDHVSMQRCYDG 271

Query: 265 LIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNHFGRGLVQRMPRCR 324
           LIDA+ GST ITISN HFT HNEVMLFGASDS + D++MQ+TLAFNHFG+ L+QRMPRCR
Sbjct: 272 LIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQITLAFNHFGKRLIQRMPRCR 331

Query: 325 WGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTKREYAMEDEWKHWS 384
           WG+ HVVNNDYTHW MYAIGGS HPTI+ QGNRFIAPP+   KQVTKREY  E  W  W+
Sbjct: 332 WGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIFKKQVTKREYNPESVWMQWT 391

Query: 385 WRSEGDELMNGAVFIPSGD-QTKGKKKGLSRYDMISFKPGTYVRRLARFSGTLECTPGKP 435
           WRSEG+  MNGA F  SGD +   K K L  YD IS  P   V  + RF+G L C PGKP
Sbjct: 392 WRSEGNLFMNGAYFTESGDPEWSSKHKDL--YDGISAAPAEDVTWMTRFAGVLGCKPGKP 449

BLAST of Pay0000233 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 1.7e-150
Identity = 260/450 (57.78%), Postives = 321/450 (71.33%), Query Frame = 0

Query: 21  NVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAA---------------------- 80
           N+ E DEYW+ RA EA++    A++ +P  + D+ +                        
Sbjct: 25  NISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDGDAKPEEE 84

Query: 81  ----VSRAMVGRNSTRRSLK--------KYRGPCLATNPIDRCWRCDRNWAKNRKKLAEC 140
               +       NSTRRSL+        K +GPC A+NPID+CWRC  +WAK RKKL  C
Sbjct: 85  EKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKRRKKLTRC 144

Query: 141 PLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQE 200
             GFGH+T GGKRG IYVVT   D+D+VNPKPGTLR+ VIQK PLWIIF   M IRL+QE
Sbjct: 145 VRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDMSIRLNQE 204

Query: 201 LMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFR 260
           L+I+S KTIDARGANVH+A GAG+T+QFV NVIIH LHIH I   SGGMIRDSVDH+G R
Sbjct: 205 LLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHISESSGGMIRDSVDHFGMR 264

Query: 261 TQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASD 320
           T++DGDG+SI+GSS++W+DH+SMS C+DGLIDAI+GST ITISN HFTHHN+VML GA +
Sbjct: 265 TRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDVMLLGAQN 324

Query: 321 SYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQG 380
           +   D+ MQVT+A+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ PTI+S G
Sbjct: 325 TNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQGPTILSHG 384

Query: 381 NRFIAPPN-PNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSR 435
           NRFIAPP+ P+ ++VTKR+YA EDEWKHW+WRS+ D  MNGA F  SG+     K   +R
Sbjct: 385 NRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQ--YKCAHTR 444

BLAST of Pay0000233 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.1e-149
Identity = 265/452 (58.63%), Postives = 322/452 (71.24%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVV--ANANVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAA 60
           + G+  +F  S L  V      NV   D YW  R ++A K    +++P+P  VT++ N  
Sbjct: 8   LGGYVFVFFSSFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYH 67

Query: 61  VSRAM---VGRNSTRRSLKKYR---------GPCLATNPIDRCWRCDRNWAKNRKKLAEC 120
           V+ A+     RN TRR L + R         G CLA NPID CWRCDRNWA NRKKLA+C
Sbjct: 68  VNIAVDASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADC 127

Query: 121 PLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQE 180
            LGFG +T GGK GPIYVV DASD+DL+NPKPGTLR+ V + GPLWIIF RSMII+L QE
Sbjct: 128 VLGFGRRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQE 187

Query: 181 LMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFR 240
           LMI+SDKTID RGA V+I  GAGLT+QFVNNVIIHN+++  I+  +GG+IRDS  H G R
Sbjct: 188 LMITSDKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLR 247

Query: 241 TQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASD 300
           T+SDGDGIS+FG++++WIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D
Sbjct: 248 TKSDGDGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARD 307

Query: 301 SYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQG 360
            +  D+ MQ+T+AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTI+SQG
Sbjct: 308 EHVIDKKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQG 367

Query: 361 NRFIAPPNPNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQ---TKGKKKGL 420
           NRFIAPPN  AKQ+TKREY    EWK W+W+SEGD  +NGA F+ SG     +   K  L
Sbjct: 368 NRFIAPPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPL 427

Query: 421 SRYDMISFKPGTYVRRLARFSGTLECTPGKPC 435
                I  KPGT VR+L   +G L C  G+ C
Sbjct: 428 PNKFTIRPKPGTMVRKLTMDAGVLGCKLGEAC 459

BLAST of Pay0000233 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.5e-149
Identity = 258/461 (55.97%), Postives = 325/461 (70.50%), Query Frame = 0

Query: 11  SLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNA-------------- 70
           SL+  + AN   + DEYW  +A EA+K    A++P+P  + D+ +               
Sbjct: 17  SLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVEGTEE 76

Query: 71  ----------AVSRAMVGRNSTRRSL------------KKYRGPCLATNPIDRCWRCDRN 130
                      +       NSTRRSL             K  GPC A+NPID+CWRC  +
Sbjct: 77  EKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPD 136

Query: 131 WAKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIF 190
           WA+ RKKL  C  GFG++T GGKRG IYVVT   DDD+VNP+PGTLR+ VIQK PLWI+F
Sbjct: 137 WARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVF 196

Query: 191 GRSMIIRLSQELMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGM 250
              M IRLSQELMI+SDKTIDARGANVHIA+GAG+T+Q+V+N+IIH LH+H I+  SGG+
Sbjct: 197 KHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGL 256

Query: 251 IRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTH 310
           IRDS++H+G R ++DGDGISIFG++++W+DH+SMS C+DGLIDAIMGSTAITISN HFTH
Sbjct: 257 IRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTH 316

Query: 311 HNEVMLFGASDSYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIG 370
           HN+VML GA ++  +D+ MQVT+A+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIG
Sbjct: 317 HNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIG 376

Query: 371 GSQHPTIVSQGNRFIAPPN-PNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGD 430
           GSQ PTI+S GNRFIAPP+  + ++VTKR+YA E EWK+W+WRSE D  MN A F  SG+
Sbjct: 377 GSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGN 436

Query: 431 QTKGKKKGLSRYDMISFKPGTYVRRLARFSGTLECTPGKPC 435
                K   SR  MI  K G  V +L +++G L+C  GK C
Sbjct: 437 --PHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Pay0000233 vs. ExPASy TrEMBL
Match: A0A1S3C3Z5 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496402 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 4.7e-260
Identity = 433/434 (99.77%), Postives = 433/434 (99.77%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR 60
           MAGFSTLFV SLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR
Sbjct: 1   MAGFSTLFVCSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR 60

Query: 61  AMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGP 120
           AMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGP
Sbjct: 61  AMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGP 120

Query: 121 IYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN 180
           IYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN
Sbjct: 121 IYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN 180

Query: 181 VHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH 240
           VHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH
Sbjct: 181 VHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH 240

Query: 241 VWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFN 300
           VWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFN
Sbjct: 241 VWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFN 300

Query: 301 HFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVT 360
           HFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVT
Sbjct: 301 HFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVT 360

Query: 361 KREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLA 420
           KREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLA
Sbjct: 361 KREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLA 420

Query: 421 RFSGTLECTPGKPC 435
           RFSGTLECTPGKPC
Sbjct: 421 RFSGTLECTPGKPC 434

BLAST of Pay0000233 vs. ExPASy TrEMBL
Match: A0A5A7UQS9 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00930 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.6e-226
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 62  MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI 121
           MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI
Sbjct: 1   MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI 60

Query: 122 YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV 181
           YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV
Sbjct: 61  YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV 120

Query: 182 HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV 241
           HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV
Sbjct: 121 HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV 180

Query: 242 WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH 301
           WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH
Sbjct: 181 WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH 240

Query: 302 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK 361
           FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK
Sbjct: 241 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK 300

Query: 362 REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR 421
           REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR
Sbjct: 301 REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR 360

Query: 422 FSGTLECTPGKPC 435
           FSGTLECTPGKPC
Sbjct: 361 FSGTLECTPGKPC 373

BLAST of Pay0000233 vs. ExPASy TrEMBL
Match: A0A0A0KG16 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G404140 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.4e-224
Identity = 380/435 (87.36%), Postives = 392/435 (90.11%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR 60
           MA FS+LFV SLLFFVVANANVDEDEYW TRATEAKKVA+ AFN NPEIVTD LNAAVSR
Sbjct: 1   MAVFSSLFVCSLLFFVVANANVDEDEYWQTRATEAKKVAQGAFNANPEIVTDTLNAAVSR 60

Query: 61  AMVGRNSTRRSL-KKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRG 120
            M+G NSTRRSL KKYRGPCLATNPIDRCWRCDRNWAKNR+KLAECPLGFGHQTIGGKRG
Sbjct: 61  TMLGHNSTRRSLRKKYRGPCLATNPIDRCWRCDRNWAKNRRKLAECPLGFGHQTIGGKRG 120

Query: 121 PIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGA 180
           PIYVVTDASDDDLVNPKPGTLR+GVIQKGPLWIIFGRSM+IRLSQELMISS KTIDARGA
Sbjct: 121 PIYVVTDASDDDLVNPKPGTLRHGVIQKGPLWIIFGRSMVIRLSQELMISSHKTIDARGA 180

Query: 181 NVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSS 240
           NVHIAFGAGLTIQFVNNVIIHNLHIHDI+SKSGGMIRDSVDHYGFRTQSDGDGISIFGSS
Sbjct: 181 NVHIAFGAGLTIQFVNNVIIHNLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSS 240

Query: 241 HVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAF 300
           HVWIDHVSMSNC+DGLIDAIMGSTAITISNCHFTHHNE  +                   
Sbjct: 241 HVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNERAIATQKTKLC----------- 300

Query: 301 NHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQV 360
                    +MPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQ+
Sbjct: 301 ---------KMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQI 360

Query: 361 TKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRL 420
           TKREYAMEDEWKHWSWRSEGDELMNGAVFI SGDQTKGKKKGLSRYDMISFKPGTYVRRL
Sbjct: 361 TKREYAMEDEWKHWSWRSEGDELMNGAVFIASGDQTKGKKKGLSRYDMISFKPGTYVRRL 415

Query: 421 ARFSGTLECTPGKPC 435
            R SGT+ECTPGKPC
Sbjct: 421 VRLSGTIECTPGKPC 415

BLAST of Pay0000233 vs. ExPASy TrEMBL
Match: A0A6J1G495 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450600 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.5e-218
Identity = 368/439 (83.83%), Postives = 395/439 (89.98%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVVA--NANV---DEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLN 60
           MAGF  LFV+SLL+FV A  +ANV   D DEYW TRA EAKKVA+ A+NP PE VT++LN
Sbjct: 1   MAGFRILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHLN 60

Query: 61  AAVSRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIG 120
            AV  AMVG NSTRRSL KY+GPCL TNPIDRCWRCD+NWA NRKKLAECPLGFGHQTIG
Sbjct: 61  VAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIG 120

Query: 121 GKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTID 180
           GKRG IYVVTDASDD+LV+PKPGTLR+GVIQKGPLWIIFGRSM+IRL+QELMISSDKTID
Sbjct: 121 GKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTID 180

Query: 181 ARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISI 240
           ARG NVHIA+GAGLTIQF +NVIIH LHIH+IISKSGG IRDSVDHYGFRTQSDGDGISI
Sbjct: 181 ARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISI 240

Query: 241 FGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQV 300
           FGSSH+WIDHVSM  C+DGLIDAIMGST ITISN HFTHHNE MLFGASDSY EDEIMQ+
Sbjct: 241 FGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQI 300

Query: 301 TLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPN 360
           TLAFNHFGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N N
Sbjct: 301 TLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNIN 360

Query: 361 AKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTY 420
           AK +TKREYA E EWK WSWRSEGD L+NGAVF+ SGDQTKGKKK LSRYDMISFKPG+Y
Sbjct: 361 AKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGSY 420

Query: 421 VRRLARFSGTLECTPGKPC 435
           V+RL RF+GTLEC  GKPC
Sbjct: 421 VKRLVRFAGTLECRVGKPC 439

BLAST of Pay0000233 vs. ExPASy TrEMBL
Match: A0A6J1KG97 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493968 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 3.8e-217
Identity = 367/436 (84.17%), Postives = 393/436 (90.14%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVV-ANANVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAV 60
           MAGF  LFV+SL FF   A+ +VD+ DEYW TRA EAK+VAK A+NPNPE VT++LN AV
Sbjct: 1   MAGFRILFVFSLYFFAAYASVDVDDHDEYWLTRANEAKEVAKDAYNPNPESVTEHLNVAV 60

Query: 61  SRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKR 120
             AMVG NSTRRSL KY+GPCL TNPIDRCWRCD+NWA NRKKLAECPLGFGHQTIGGKR
Sbjct: 61  RLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIGGKR 120

Query: 121 GPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARG 180
           G IYVVTDASDD+LV+PK GTLRYGVIQKGPLWIIFGRSM+IRL+QELMISSDKTIDARG
Sbjct: 121 GRIYVVTDASDDNLVDPKLGTLRYGVIQKGPLWIIFGRSMVIRLAQELMISSDKTIDARG 180

Query: 181 ANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240
            NVHIA+GAGLTIQF +NVIIH LHIH+IISKSGG IRDSVDHYGFRTQSDGDGISIFGS
Sbjct: 181 FNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISIFGS 240

Query: 241 SHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLA 300
           SHVWIDHVSM  C+DGLIDAIMGST ITISN HFTHHNE MLFGASDSY EDEIMQ+TLA
Sbjct: 241 SHVWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQITLA 300

Query: 301 FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQ 360
           FNHFGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N NAK 
Sbjct: 301 FNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNINAKT 360

Query: 361 VTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRR 420
           +TKREYA E EWK WSWRSEGD LMNGAVF+ SGDQTKGKKK LSRYDMIS+KPG+YV+R
Sbjct: 361 ITKREYADESEWKKWSWRSEGDLLMNGAVFVGSGDQTKGKKKDLSRYDMISYKPGSYVKR 420

Query: 421 LARFSGTLECTPGKPC 435
           L RF+GTLEC  GKPC
Sbjct: 421 LVRFAGTLECRVGKPC 436

BLAST of Pay0000233 vs. NCBI nr
Match: XP_008456459.1 (PREDICTED: pectate lyase-like [Cucumis melo])

HSP 1 Score: 906.4 bits (2341), Expect = 9.7e-260
Identity = 433/434 (99.77%), Postives = 433/434 (99.77%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR 60
           MAGFSTLFV SLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR
Sbjct: 1   MAGFSTLFVCSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR 60

Query: 61  AMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGP 120
           AMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGP
Sbjct: 61  AMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGP 120

Query: 121 IYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN 180
           IYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN
Sbjct: 121 IYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN 180

Query: 181 VHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH 240
           VHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH
Sbjct: 181 VHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH 240

Query: 241 VWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFN 300
           VWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFN
Sbjct: 241 VWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFN 300

Query: 301 HFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVT 360
           HFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVT
Sbjct: 301 HFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVT 360

Query: 361 KREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLA 420
           KREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLA
Sbjct: 361 KREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLA 420

Query: 421 RFSGTLECTPGKPC 435
           RFSGTLECTPGKPC
Sbjct: 421 RFSGTLECTPGKPC 434

BLAST of Pay0000233 vs. NCBI nr
Match: XP_004146792.2 (pectate lyase [Cucumis sativus] >KAE8647292.1 hypothetical protein Csa_003655 [Cucumis sativus])

HSP 1 Score: 865.5 bits (2235), Expect = 1.9e-247
Identity = 412/435 (94.71%), Postives = 422/435 (97.01%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR 60
           MA FS+LFV SLLFFVVANANVDEDEYW TRATEAKKVA+ AFN NPEIVTD LNAAVSR
Sbjct: 1   MAVFSSLFVCSLLFFVVANANVDEDEYWQTRATEAKKVAQGAFNANPEIVTDTLNAAVSR 60

Query: 61  AMVGRNSTRRSL-KKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRG 120
            M+G NSTRRSL KKYRGPCLATNPIDRCWRCDRNWAKNR+KLAECPLGFGHQTIGGKRG
Sbjct: 61  TMLGHNSTRRSLRKKYRGPCLATNPIDRCWRCDRNWAKNRRKLAECPLGFGHQTIGGKRG 120

Query: 121 PIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGA 180
           PIYVVTDASDDDLVNPKPGTLR+GVIQKGPLWIIFGRSM+IRLSQELMISS KTIDARGA
Sbjct: 121 PIYVVTDASDDDLVNPKPGTLRHGVIQKGPLWIIFGRSMVIRLSQELMISSHKTIDARGA 180

Query: 181 NVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSS 240
           NVHIAFGAGLTIQFVNNVIIHNLHIHDI+SKSGGMIRDSVDHYGFRTQSDGDGISIFGSS
Sbjct: 181 NVHIAFGAGLTIQFVNNVIIHNLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSS 240

Query: 241 HVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAF 300
           HVWIDHVSMSNC+DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAF
Sbjct: 241 HVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAF 300

Query: 301 NHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQV 360
           NHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQ+
Sbjct: 301 NHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQI 360

Query: 361 TKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRL 420
           TKREYAMEDEWKHWSWRSEGDELMNGAVFI SGDQTKGKKKGLSRYDMISFKPGTYVRRL
Sbjct: 361 TKREYAMEDEWKHWSWRSEGDELMNGAVFIASGDQTKGKKKGLSRYDMISFKPGTYVRRL 420

Query: 421 ARFSGTLECTPGKPC 435
            R SGT+ECTPGKPC
Sbjct: 421 VRLSGTIECTPGKPC 435

BLAST of Pay0000233 vs. NCBI nr
Match: XP_038886315.1 (pectate lyase-like [Benincasa hispida])

HSP 1 Score: 840.1 bits (2169), Expect = 8.6e-240
Identity = 396/434 (91.24%), Postives = 415/434 (95.62%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAVSR 60
           M GF TLFV  LLFFVVANA+VDEDEYW TRATEA+KVA+ A+NP+PEIVTDNLNAAVSR
Sbjct: 1   MVGFRTLFVCLLLFFVVANADVDEDEYWRTRATEAQKVAQDAYNPDPEIVTDNLNAAVSR 60

Query: 61  AMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGP 120
            M+GRNSTRRSL  Y+GPCLATNPIDRCWRC+RNWA+NRKKLAECPLGFGH+TIGGKRGP
Sbjct: 61  VMIGRNSTRRSL-NYKGPCLATNPIDRCWRCNRNWARNRKKLAECPLGFGHKTIGGKRGP 120

Query: 121 IYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN 180
           IYVVTDASDDDL+NPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN
Sbjct: 121 IYVVTDASDDDLINPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGAN 180

Query: 181 VHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH 240
           VHIA+GAGLTIQFVNNVIIHNLHIHDI+SKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH
Sbjct: 181 VHIAYGAGLTIQFVNNVIIHNLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSH 240

Query: 241 VWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFN 300
           VWIDH+SMSNC+DGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAED IMQ+TLAFN
Sbjct: 241 VWIDHISMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDAIMQITLAFN 300

Query: 301 HFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVT 360
           HFGRGLVQRMPRCRWGFFHVVNN YTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK VT
Sbjct: 301 HFGRGLVQRMPRCRWGFFHVVNNHYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKLVT 360

Query: 361 KREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLA 420
           KREYAME EWK WSWRSEGD L+NGAVFIPSGDQTKGK+KGLSRYDMISFKPGTYVRRL 
Sbjct: 361 KREYAMESEWKKWSWRSEGDALVNGAVFIPSGDQTKGKRKGLSRYDMISFKPGTYVRRLV 420

Query: 421 RFSGTLECTPGKPC 435
           RF+GTL C P KPC
Sbjct: 421 RFAGTLACAPRKPC 433

BLAST of Pay0000233 vs. NCBI nr
Match: KAA0055829.1 (pectate lyase-like [Cucumis melo var. makuwa] >TYK00045.1 pectate lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 792.7 bits (2046), Expect = 1.6e-225
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 62  MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI 121
           MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI
Sbjct: 1   MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI 60

Query: 122 YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV 181
           YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV
Sbjct: 61  YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV 120

Query: 182 HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV 241
           HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV
Sbjct: 121 HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV 180

Query: 242 WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH 301
           WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH
Sbjct: 181 WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH 240

Query: 302 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK 361
           FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK
Sbjct: 241 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK 300

Query: 362 REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR 421
           REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR
Sbjct: 301 REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR 360

Query: 422 FSGTLECTPGKPC 435
           FSGTLECTPGKPC
Sbjct: 361 FSGTLECTPGKPC 373

BLAST of Pay0000233 vs. NCBI nr
Match: XP_023523501.1 (pectate lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.1 bits (1995), Expect = 1.3e-219
Identity = 370/437 (84.67%), Postives = 398/437 (91.08%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVVA--NANVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAA 60
           MAGF TLFV+SLL+FV A  +ANVD+ DEYW TRA EAKKVA+ A+NPNPE VT++LN A
Sbjct: 1   MAGFRTLFVFSLLYFVAACTDANVDDHDEYWLTRANEAKKVAQDAYNPNPESVTEHLNVA 60

Query: 61  VSRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGK 120
           V  A+VG NSTRRSL KY+GPCL TNPIDRCWRCD+NWAKNRKKLAECPLGFGHQTIGGK
Sbjct: 61  VHLAIVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWAKNRKKLAECPLGFGHQTIGGK 120

Query: 121 RGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDAR 180
           RG IYVVTDASDD+LV+PKPGTLRYGVIQKGPLWIIFGRSM+IRL+QELMISSDKTIDAR
Sbjct: 121 RGRIYVVTDASDDNLVDPKPGTLRYGVIQKGPLWIIFGRSMVIRLAQELMISSDKTIDAR 180

Query: 181 GANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFG 240
           G NVHIA+GAGLTIQF +NVIIH LHIH+IISKSGG IRDSVDHYGFRTQSDGDGISIFG
Sbjct: 181 GFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISIFG 240

Query: 241 SSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTL 300
           SSHVWIDHVSM  C+DGLIDAIMGST ITISN HFTHHNE MLFGASDSY EDEIMQ+TL
Sbjct: 241 SSHVWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQITL 300

Query: 301 AFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK 360
           AFNHFGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N NAK
Sbjct: 301 AFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNINAK 360

Query: 361 QVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVR 420
            +TKREYA E EWK WSWRSEGD  +NGAVF+ SGDQTKGKKK L+RYDMISFKPG+YV+
Sbjct: 361 TITKREYADESEWKKWSWRSEGDLFLNGAVFVGSGDQTKGKKKDLTRYDMISFKPGSYVK 420

Query: 421 RLARFSGTLECTPGKPC 435
           RL RF+GTLEC  GKPC
Sbjct: 421 RLVRFAGTLECRVGKPC 437

BLAST of Pay0000233 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 533.5 bits (1373), Expect = 1.6e-151
Identity = 259/450 (57.56%), Postives = 321/450 (71.33%), Query Frame = 0

Query: 21  NVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAA---------------------- 80
           N+ E DEYW+ RA EA++    A++ +P  + D+ +                        
Sbjct: 25  NISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDGDAKPEEE 84

Query: 81  ----VSRAMVGRNSTRRSLK--------KYRGPCLATNPIDRCWRCDRNWAKNRKKLAEC 140
               +       NSTRRSL+        K +GPC A+NPID+CWRC  +WAK RKKL  C
Sbjct: 85  EKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKRRKKLTRC 144

Query: 141 PLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQE 200
             GFGH+T GGKRG IYVVT   D+D+VNPKPGTLR+ VIQK PLWIIF   M IRL+QE
Sbjct: 145 VRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDMSIRLNQE 204

Query: 201 LMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFR 260
           L+I+S KTIDARGANVH+A GAG+T+QFV NV+IH LHIH I   SGGMIRDSVDH+G R
Sbjct: 205 LLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISESSGGMIRDSVDHFGMR 264

Query: 261 TQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASD 320
           T++DGDG+SI+GSS++W+DH+SMS C+DGLIDAI+GST ITISN HFTHHN+VML GA +
Sbjct: 265 TRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDVMLLGAQN 324

Query: 321 SYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQG 380
           +   D+ MQVT+A+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ PTI+S G
Sbjct: 325 TNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQGPTILSHG 384

Query: 381 NRFIAPPN-PNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSR 435
           NRFIAPP+ P+ ++VTKR+YA EDEWKHW+WRS+ D  MNGA F  SG+     K   +R
Sbjct: 385 NRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQ--YKCAHTR 444

BLAST of Pay0000233 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 531.2 bits (1367), Expect = 7.9e-151
Identity = 265/452 (58.63%), Postives = 322/452 (71.24%), Query Frame = 0

Query: 1   MAGFSTLFVWSLLFFVV--ANANVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAA 60
           + G+  +F  S L  V      NV   D YW  R ++A K    +++P+P  VT++ N  
Sbjct: 8   LGGYVFVFFSSFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYH 67

Query: 61  VSRAM---VGRNSTRRSLKKYR---------GPCLATNPIDRCWRCDRNWAKNRKKLAEC 120
           V+ A+     RN TRR L + R         G CLA NPID CWRCDRNWA NRKKLA+C
Sbjct: 68  VNIAVDASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADC 127

Query: 121 PLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQE 180
            LGFG +T GGK GPIYVV DASD+DL+NPKPGTLR+ V + GPLWIIF RSMII+L QE
Sbjct: 128 VLGFGRRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQE 187

Query: 181 LMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFR 240
           LMI+SDKTID RGA V+I  GAGLT+QFVNNVIIHN+++  I+  +GG+IRDS  H G R
Sbjct: 188 LMITSDKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLR 247

Query: 241 TQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASD 300
           T+SDGDGIS+FG++++WIDHVSM+ C DG+IDAI GSTA+TISN HFT H EVMLFGA D
Sbjct: 248 TKSDGDGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARD 307

Query: 301 SYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQG 360
            +  D+ MQ+T+AFNHFG+ L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTI+SQG
Sbjct: 308 EHVIDKKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQG 367

Query: 361 NRFIAPPNPNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQ---TKGKKKGL 420
           NRFIAPPN  AKQ+TKREY    EWK W+W+SEGD  +NGA F+ SG     +   K  L
Sbjct: 368 NRFIAPPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPL 427

Query: 421 SRYDMISFKPGTYVRRLARFSGTLECTPGKPC 435
                I  KPGT VR+L   +G L C  G+ C
Sbjct: 428 PNKFTIRPKPGTMVRKLTMDAGVLGCKLGEAC 459

BLAST of Pay0000233 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 530.8 bits (1366), Expect = 1.0e-150
Identity = 258/461 (55.97%), Postives = 325/461 (70.50%), Query Frame = 0

Query: 11  SLLFFVVANANVDEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLNA-------------- 70
           SL+  + AN   + DEYW  +A EA+K    A++P+P  + D+ +               
Sbjct: 17  SLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVEGTEE 76

Query: 71  ----------AVSRAMVGRNSTRRSL------------KKYRGPCLATNPIDRCWRCDRN 130
                      +       NSTRRSL             K  GPC A+NPID+CWRC  +
Sbjct: 77  EKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPD 136

Query: 131 WAKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIF 190
           WA+ RKKL  C  GFG++T GGKRG IYVVT   DDD+VNP+PGTLR+ VIQK PLWI+F
Sbjct: 137 WARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVF 196

Query: 191 GRSMIIRLSQELMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGM 250
              M IRLSQELMI+SDKTIDARGANVHIA+GAG+T+Q+V+N+IIH LH+H I+  SGG+
Sbjct: 197 KHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGL 256

Query: 251 IRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTH 310
           IRDS++H+G R ++DGDGISIFG++++W+DH+SMS C+DGLIDAIMGSTAITISN HFTH
Sbjct: 257 IRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTH 316

Query: 311 HNEVMLFGASDSYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIG 370
           HN+VML GA ++  +D+ MQVT+A+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIG
Sbjct: 317 HNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIG 376

Query: 371 GSQHPTIVSQGNRFIAPPN-PNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGD 430
           GSQ PTI+S GNRFIAPP+  + ++VTKR+YA E EWK+W+WRSE D  MN A F  SG+
Sbjct: 377 GSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGN 436

Query: 431 QTKGKKKGLSRYDMISFKPGTYVRRLARFSGTLECTPGKPC 435
                K   SR  MI  K G  V +L +++G L+C  GK C
Sbjct: 437 --PHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Pay0000233 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 506.1 bits (1302), Expect = 2.7e-143
Identity = 256/450 (56.89%), Postives = 319/450 (70.89%), Query Frame = 0

Query: 5   STLFVWSLLFFVVANANV-----DEDEYWNTRATEAKKVAKSAFNPNPEIVTDNLN--AA 64
           S +FV+  L   V   +V     + DEYW  R T A +    +++PNPE VTD+ N  AA
Sbjct: 6   SYVFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAA 65

Query: 65  VSRAMVG-RNSTRRSLK---------KYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPL 124
           ++    G  N TRR L+         +  G   + N ID+CWR D+NW KNRKKLA+C L
Sbjct: 66  LAMETTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVL 125

Query: 125 GFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELM 184
           GFG +T GGK GPIYVVTD SD+DL+ PKPGT+R+ V +  PLWIIF RSMII+L QEL+
Sbjct: 126 GFGRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELI 185

Query: 185 ISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQ 244
           I++DKTID RGA ++I  GAGLT+QFV NVIIHN+HI  I   +GG+I DS  H+G RT 
Sbjct: 186 ITNDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTV 245

Query: 245 SDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSY 304
           SDGDGI+IFG+++VWIDHVSM++C DG+IDAIMGSTAITISN HFT H+EVMLFG ++  
Sbjct: 246 SDGDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKD 305

Query: 305 AEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNR 364
             D+ MQ+T+AFNHFG+ L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTI+SQGNR
Sbjct: 306 VIDKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNR 365

Query: 365 FIAPPNPNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQ---TKGKKKGLSR 424
           FIAPP  ++KQVTKREY    EWK W+W+SE D  +NGA F+ SG     +   K  + R
Sbjct: 366 FIAPPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPR 425

Query: 425 YDMISFKPGTYVRRLARFSGTLECTPGKPC 435
              I  +PGT VRRL + +GTL C PGK C
Sbjct: 426 KFAIRPQPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of Pay0000233 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.6e-138
Identity = 234/405 (57.78%), Postives = 289/405 (71.36%), Query Frame = 0

Query: 30  TRATEAKKVAKSAFNPNPEIVTDNLNAAVSRAMVGRNSTRRSLKKYRGPCLATNPIDRCW 89
           TR     +  + A   +P++V D +   V  +   R + RR L  +   C   NPID CW
Sbjct: 72  TRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNR-TARRKLGFF--SCGTGNPIDDCW 131

Query: 90  RCDRNWAKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDDDLVNPKPGTLRYGVIQKGP 149
           RCDRNW KNRK+LA+C +GFG   IGG+ G  Y+VTD +D+D+VNPKPGTLR+ VIQ+ P
Sbjct: 132 RCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEP 191

Query: 150 LWIIFGRSMIIRLSQELMISSDKTIDARGANVHIAFGAGLTIQFVNNVIIHNLHIHDIIS 209
           LWI+F R M+I L QEL+++S KTIDARG+NVHIA GA +TIQF+ NVIIH LHIHD   
Sbjct: 192 LWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKP 251

Query: 210 KSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCEDGLIDAIMGSTAITISN 269
               M+R S  H+G+RT +DGD +SIFGSSH+WIDH S+S+C DGL+DA+MGSTAIT+SN
Sbjct: 252 TGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSN 311

Query: 270 CHFTHHNEVMLFGASDSYAEDEIMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWL 329
            HFTHHNEVML G SDSY +D++MQVT+A+NHFG GLVQRMPRCR G+FHVVNNDYTHW 
Sbjct: 312 NHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 371

Query: 330 MYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTKREYAMEDEWKHWSWRSEGDELMNGAVFI 389
           MYAIGGS  PTI SQGNR+ AP +  AK+VTKR      EWK W+WRSEGD L+NGA F 
Sbjct: 372 MYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFR 431

Query: 390 PSGDQTKGKKKGLSRYDMISFKPGTYVRRLARFSGTLECTPGKPC 435
           PSG    G      R   ++ KP + V  +   +G L C  G+PC
Sbjct: 432 PSG---AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P409731.8e-16063.19Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1[more]
P157224.2e-15764.13Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1[more]
Q9LFP51.7e-15057.78Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1[more]
Q9M9S21.1e-14958.63Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1[more]
Q9SRH41.5e-14955.97Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C3Z54.7e-26099.77Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496402 PE=3 SV=1[more]
A0A5A7UQS97.6e-226100.00Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00930 ... [more]
A0A0A0KG162.4e-22487.36Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_6G404140 PE=3 SV=1[more]
A0A6J1G4954.5e-21883.83Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450600 PE=3 SV=1[more]
A0A6J1KG973.8e-21784.17Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493968 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008456459.19.7e-26099.77PREDICTED: pectate lyase-like [Cucumis melo][more]
XP_004146792.21.9e-24794.71pectate lyase [Cucumis sativus] >KAE8647292.1 hypothetical protein Csa_003655 [C... [more]
XP_038886315.18.6e-24091.24pectate lyase-like [Benincasa hispida][more]
KAA0055829.11.6e-225100.00pectate lyase-like [Cucumis melo var. makuwa] >TYK00045.1 pectate lyase-like [Cu... [more]
XP_023523501.11.3e-21984.67pectate lyase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G15110.11.6e-15157.56Pectate lyase family protein [more]
AT1G14420.17.9e-15158.63Pectate lyase family protein [more]
AT3G01270.11.0e-15055.97Pectate lyase family protein [more]
AT2G02720.12.7e-14356.89Pectate lyase family protein [more]
AT4G13710.12.6e-13857.78Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 105..122
score: 57.94
coord: 331..350
score: 77.86
coord: 373..397
score: 48.95
coord: 129..154
score: 71.06
coord: 229..250
score: 64.94
coord: 309..328
score: 78.1
coord: 165..181
score: 67.23
coord: 405..428
score: 35.71
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 157..354
e-value: 5.4E-103
score: 358.1
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 167..348
e-value: 1.9E-21
score: 76.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 82..427
e-value: 2.6E-115
score: 387.0
IPR007524Pectate lyase, N-terminalPFAMPF04431Pec_lyase_Ncoord: 25..72
e-value: 2.3E-16
score: 59.8
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 11..434
NoneNo IPR availablePANTHERPTHR31683:SF132PECTATE LYASEcoord: 11..434
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 72..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0000233.1Pay0000233.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030570 pectate lyase activity