Pay0000070 (gene) Melon (Payzawat) v1

Overview


Name	Pay0000070
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	tRNA pseudouridine synthase
Location	chr11: 29735414 .. 29737874 (+)
RNA-Seq Expression	Pay0000070
Synteny	Pay0000070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCAATTTCCCTTCCCTTTTCACCAATCATTCCATCTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGGTAACGACAATTATTTTTCTGATCTCTCAGTTCCTCTTTTCATCTTGGTGCTTTAATTTTGACGATATTCATTTGATCGTGCCTTGAAAAGAATGCGGAGGATTCCTCTTATATCACAGAGCCCCCTTTCCCCAGCTTCAAATGGCGGTTGCTCATCGCTTATGACGGTACTCGATATGCAGGTCCAAGTTTCTTTTTCTTCTCTCTAATTGTCTATTCAGGCGAATGAATTGAACGATTTTGTGTTCGTGTGCTAGCTTTATCTCTTTGGGTTAACAGTAATTGTAAATAGCCTAAGGTTTTGAGGTTAGTTGTTGTTGCCTGTTGGATGTCTTTCATCACCAGCATCAATTTCACTTCAGGATCACCCTAAAGGGAGACTCGTTTAAAAGTACTTAGCCTGCTTTTATTACGAGTTAATTCGGAGAAGTTTGTGCTCCCTGCCCAAAATTTGAGGCTTATTTGTTACATGATATAATATTAAACTTATATTTATCCATAAGTTTATGTTTTTGGATCAATCGGTCCAAGGTTTGTTGTGTTTAAACCCTTGCAGTTTATTTTTCCTCCCATTAATATTGACTCACAAGTGAGAGAGTGTTAGATATTGTAATATTAAATTTATCTTCATTCATAAGCTTGAGCCGTTGGGTCAGTCGGTAATTGAAGACTTGGTACATAGGTTTGATAAAATAGTTTTGAATATGTTGATATTAAACTAGGCTGGCAATATCAGAGGTCACCACCCACCATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTCGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTCTGATGTGATTTTATGGAGTTGGGTTAAAACACATCATTGGAATACAATGCTTGCTGTTGCTTACGACAAGTTGAGGTTTCAGGTGGCTCACTTTGTTACACCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGCACCTGATTTTCATGCTCGGTTTTCTACCTTAAGCAAGGTTTATCACTACAAGATATATAATGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAACAATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGTAAAGTTTTAAGGTTCTTTAGCCAGTTATTTGTTTGGTTAGAATTACCAATTTAGTCTATAAACTTTCTGTCTATTTGGTCATCTTTCAAAAGTGTTCAATAGGTCATTGCACTTCAATCTTGTACCATATAGGACGTCGAACTTTAAAATGTTTTGAAAGGTTCTTAAACTTTCAAATTCATGTTTATTAAATCTCTTATTTTCGGTTTTGTGTTTACATTGACATAATATTCAAATTTATATATTGTAGGTCAATTAGTTAAAAAAAATGAATTTGCCAAGGACTTATTAGGCACAAAATTGATAGTTTAAGGGTCTATCAAACATTTTTAACTGATCGAGATCCATTTGATACATTTGCAAGTTCAAGACCAATAGATTCAAACATTAGTCTATGGACTAAATTTACAGTCTAATCAGCTTCTTTTGTAATTTTTTTTATCCCTATGATGTGTCTGCTTCTCAGGTCTAAAGTTGCTAATTCTGTATGCCCTTCTTTTGATCATCTGTGTGAGTGTGAATCATTCCGGTTTCAGCTTTTTACAAAATCTATGCAACTAAATGCTTTCTTTTGTTCAGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTAATTTTTGTTATATCTATCTTTTCAGTCAAAAGTCCCTTCTCTGATTGTTTTATTTTTACTTATGCTGGTTGCTAAAATGGCTTTATAGTCCTGGAAGCATAGATTTAAGTTTGTAAAATCGGTTTCATGCAAGGATTTTCTCTTGTGTGTTTTCAGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAAAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCTCAGGGACTGTGTCTTGTGAGTATAAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTCTTGGTAGGAGTCGTAGTGTGGGCAAATGTAAAGTCCCTTTTTATTGA

mRNA sequence

ATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCAATTTCCCTTCCCTTTTCACCAATCATTCCATCTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGAATGCGGAGGATTCCTCTTATATCACAGAGCCCCCTTTCCCCAGCTTCAAATGGCGGTTGCTCATCGCTTATGACGGTACTCGATATGCAGGCTGGCAATATCAGAGGTCACCACCCACCATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTCGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTGGCTCACTTTGTTACACCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGCACCTGATTTTCATGCTCGGTTTTCTACCTTAAGCAAGGTTTATCACTACAAGATATATAATGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAACAATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAAAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCTCAGGGACTGTGTCTTGTGAGTATAAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTCTTGGTAGGAGTCGTAGTGTGGGCAAATGTAAAGTCCCTTTTTATTGA

Coding sequence (CDS)

ATGAGGTGCTGGGCGGTGGGTTCGCCTGTTCTTCCTCCAATTTCCCTTCCCTTTTCACCAATCATTCCATCTCCAAATGGCGCTTCATCTTCCTCCAGTCGTGGATTCCCGAATGCGGAGGATTCCTCTTATATCACAGAGCCCCCTTTCCCCAGCTTCAAATGGCGGTTGCTCATCGCTTATGACGGTACTCGATATGCAGGCTGGCAATATCAGAGGTCACCACCCACCATCCAGTGCCTCATTGAAGAAGCCTTGATTGGAGCAACAAAGCTTGAAAGGAAGGATCTTCATTTAGTCGGTGCAAGCAGAACAGATAAAGGAGTACATGCCTGGGGTCAGGTGGCTCACTTTGTTACACCATTTAACTACGATAGCTTGGAAAGCGTTCATGCTGCACTGAATGGCCTTCTTCCTGTGGATATAAGAGTTCGAGAGATGAGTCCTGCTGCACCTGATTTTCATGCTCGGTTTTCTACCTTAAGCAAGGTTTATCACTACAAGATATATAATGGCACAATCATGGATCCATTTCAGCGTTGCTACACTTACCACTGTGTTTATAAACTCAACTATGCAGCCATGAAAGAAGCTGCAAATTATTTCGTTGGAAAGCATGATTTTTCTGCTTTTACAAATGCAGCTCATAACAATCGAGTGGTAAATCCAGTGAAGGTCATTTTCCGTTTTGATGTGATAGAAATGGGAGCTCTTGTGCAGCTAGAAGTTGAGGGTTCTGGGTTCTTATATAGACAAGTTCGGAATATGGTTGGCTTGTTGGTTCAAATTGGAAAGGAAGCACTTCCTCCTGAGGTTGTTCCTAAGATTTTGGCATCTCGAGATCGCAAAGAGCTTGCCAAGTATGCTTCGGGCGTTCCTCCTCAGGGACTGTGTCTTGTGAGTATAAATTATAAAGAGGACCATTTAAAGCTTCCATCTGATTGCCCTGGGACTAGTCTTGGTAGGAGTCGTAGTGTGGGCAAATGTAAAGTCCCTTTTTATTGA

Protein sequence

MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY

Homology

[Close]

BLAST of Pay0000070 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 107/255 (41.96%), Postives = 164/255 (64.31%), Query Frame = 0


Query: 55  WRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQ 114
           ++L IAYDGT Y+GWQ Q +  +IQ  I+EAL     L+++ + LVG+ RTD GVHA GQ
Sbjct: 4   YKLTIAYDGTNYSGWQIQPNASSIQQKIQEAL--CILLKKEKVVLVGSGRTDAGVHAKGQ 63

Query: 115 VAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTI 174
           VAHF    +Y  L  +H +LNGLLP DIR++ + P +P FH+++S + K YHY ++   +
Sbjct: 64  VAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV 123

Query: 175 MDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVV-NPVKVIFRFDVI 234
           MDPFQR Y++H   K++   +K+AA  F G HDF++F N AH      NPV+ ++R D+ 
Sbjct: 124 MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK 183

Query: 235 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 294
                ++LE EG GFLY+ VRN+VG L+ +       E + +I A+++R++ +  A   P
Sbjct: 184 PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---P 243

Query: 295 PQGLCLVSINYKEDH 309
           P+GL L+ + Y+ ++
Sbjct: 244 PEGLFLIQVFYENEN 252

[Close]

BLAST of Pay0000070 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.7e-47
Identity = 110/251 (43.82%), Postives = 142/251 (56.57%), Query Frame = 0


Query: 56  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 115
           RL +AYDGT Y G+Q Q     PTIQ  +E  L    +L    L + GASRTD GVHA G
Sbjct: 5   RLTLAYDGTAYHGFQKQSGSGLPTIQETLERCL---AELSGAALKVTGASRTDAGVHARG 64

Query: 116 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 175
           QV +FVT       E + AALNG+LP DI   +      DFHAR+S ++K Y Y +YN  
Sbjct: 65  QVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHP 124

Query: 176 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 235
           +  PFQR Y+ H    L+  AM+ AA + +G+HDFS F   A    V + V+ + R D+ 
Sbjct: 125 VRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTF--QAAGRPVQSAVRTLTRADIE 184

Query: 236 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 295
               LV+L     GFLY  VR M G L+++G E L PE +P I+AS DR   A+    VP
Sbjct: 185 AQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVP 244

Query: 296 PQGLCLVSINY 305
           P GLCL  + Y
Sbjct: 245 PHGLCLEEVEY 247

[Close]

BLAST of Pay0000070 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.1e-47
Identity = 111/248 (44.76%), Postives = 146/248 (58.87%), Query Frame = 0


Query: 57  LLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVA 116
           L+I YDGT Y GWQYQ++  T+Q ++E+A+    K+  + ++L+G+SRTD GVHA  QVA
Sbjct: 2   LIIEYDGTNYHGWQYQKNAITVQEVLEKAI---EKVTGEKVNLIGSSRTDTGVHALYQVA 61

Query: 117 HFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMD 176
           +F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I N     
Sbjct: 62  NFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPM 121

Query: 177 PFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMG 236
           P  R Y +H  Y L+  AMK+AA +F G HDFSAF   A  N+ VNPVK IF   V + G
Sbjct: 122 PTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTVEKEG 181

Query: 237 ALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQG 296
            L+++EVE  GFLY  VR +VG LV +G   +    +P I+ S+DR    K     PPQG
Sbjct: 182 DLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITAPPQG 240

Query: 297 LCLVSINY 305
           L LV I Y
Sbjct: 242 LYLVRIVY 240

[Close]

BLAST of Pay0000070 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 103/251 (41.04%), Postives = 146/251 (58.17%), Query Frame = 0


Query: 56  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 115
           +L +AYDGT Y G+Q QR     TIQ  +E+AL   + + +  + ++GA RTD GVHA G
Sbjct: 5   KLTLAYDGTNYHGFQEQRGTGLATIQEALEKAL---STIAQTPIQVIGAGRTDAGVHAQG 64

Query: 116 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGT 175
           QV +F +       E    ALN LLP DI+V +      DFHARFS ++K Y Y IY+  
Sbjct: 65  QVVNFRSEKWPVPAEKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHR 124

Query: 176 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVI 235
           +M PF R Y YH   +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+I
Sbjct: 125 VMSPFHRYYCYHEPRRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADII 184

Query: 236 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVP 295
           E   ++ L + G+GFLY  VR + G L+ IG   + PE + KI+ S++R      A   P
Sbjct: 185 EDAPVINLYLRGNGFLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---P 244

Query: 296 PQGLCLVSINY 305
           PQGLCL+ + Y
Sbjct: 245 PQGLCLMEVEY 247

[Close]

BLAST of Pay0000070 vs. ExPASy Swiss-Prot
Match: A3DJK8 (tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=truA PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.8e-44
Identity = 100/249 (40.16%), Postives = 144/249 (57.83%), Query Frame = 0


Query: 56  RLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQV 115
           +L+I YDGT Y GWQ Q++  T+Q  IE A+ G T    + + L+GASRTD GVHA GQV
Sbjct: 5   KLIIEYDGTNYHGWQKQKNAKTVQDTIERAIKGLT---GEKVDLIGASRTDFGVHALGQV 64

Query: 116 AHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIM 175
           A+F+T       +    ALN +LP DI ++E      DFHAR+    K Y Y IYN    
Sbjct: 65  ANFITNSGIPG-DRFSYALNRMLPDDIVIKESQEVDMDFHARYKAKGKRYRYLIYNSQFP 124

Query: 176 DPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEM 235
               R  TYH  +KL++ +M+ AA+YF+G HDFSAF ++  + +    V+ I    +   
Sbjct: 125 SALLRHRTYHVSHKLDFESMQRAASYFLGTHDFSAFRSSGSSAK--TSVRTITDVSLERE 184

Query: 236 GALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQ 295
             +V+ E+ G GFLY  VR +VG L+++G   +  + +P+I+ SR RK   + A   P +
Sbjct: 185 DKIVKFEIAGDGFLYNMVRIIVGTLMEVGIGKIRADEIPQIIESRKRKRAGRTA---PAE 244

Query: 296 GLCLVSINY 305
           GL LV + Y
Sbjct: 245 GLYLVEVYY 244

[Close]

BLAST of Pay0000070 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.1e-195
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
           SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of Pay0000070 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.1e-187
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLG 322
           SINYKEDHLKLPSDCPGTSLG
Sbjct: 301 SINYKEDHLKLPSDCPGTSLG 321

[Close]

BLAST of Pay0000070 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 9.7e-181
Identity = 312/328 (95.12%), Postives = 318/328 (96.95%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180
           TPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

Query: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVG 328
           VSINYKEDHLKLPSDCPGTS G   ++G
Sbjct: 301 VSINYKEDHLKLPSDCPGTSFGCPNNLG 328

[Close]

BLAST of Pay0000070 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.3e-168
Identity = 284/334 (85.03%), Postives = 303/334 (90.72%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           M CWAVGS VLP +  P SP IPS  G  SS+ RGFPN+EDSS + EP FPSFKWRL+IA
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIPS-RGTLSSTGRGFPNSEDSSCVPEPDFPSFKWRLVIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSL+ +HAALNGLLP DIRVRE+SPA PDFHARFSTLSKVYHYKIYN  I+DPFQR
Sbjct: 121 PFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHN+RVVNPVKVIFRFDVIEMGAL+Q
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMV LL+QIGKEA+PP+VVPKILASRDRK LAKY + +PPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
            INYKEDHLKLPSDCPGTS GRS SV KCK+PFY
Sbjct: 301 RINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 333

[Close]

BLAST of Pay0000070 vs. ExPASy TrEMBL
Match: A0A6J1H621 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 6.1e-167
Identity = 281/334 (84.13%), Postives = 302/334 (90.42%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           M  WAVGS VLP +    SP IPS NG SS + RGFP +EDSS +TEP FPSFKWRL+IA
Sbjct: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSCVTEPDFPSFKWRLVIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCL+EEAL GAT+LERKDLHLVGASRTD GVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFIT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSL+ +HAALNGLLP DIRVREMSPA PDFHARFSTLSKVYHYKIYN  I+DPFQR
Sbjct: 121 PFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHN+RVVNPVKVIFRFDVIEMGAL+Q
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMV LL+QIGKEA+PP++VPKILA+RDRKELAKY + +PPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
            INYKEDHLKLPSDCPGTS GRS SV KCK+PFY
Sbjct: 301 RINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 334

[Close]

BLAST of Pay0000070 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 692.2 bits (1785), Expect = 2.2e-195
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
           SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of Pay0000070 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 665.2 bits (1715), Expect = 2.9e-187
Identity = 322/335 (96.12%), Postives = 327/335 (97.61%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140

Query: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180
           TPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200

Query: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320

Query: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
           VSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

[Close]

BLAST of Pay0000070 vs. NCBI nr
Match: TYK02989.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa])

HSP 1 Score: 664.1 bits (1712), Expect = 6.5e-187
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLG 322
           SINYKEDHLKLPSDCPGTSLG
Sbjct: 301 SINYKEDHLKLPSDCPGTSLG 321

[Close]

BLAST of Pay0000070 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 654.1 bits (1686), Expect = 6.7e-184
Identity = 312/334 (93.41%), Postives = 324/334 (97.01%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRG PNAEDSS I  PPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVI+MG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIDMGPLVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
           SINYKEDHLKLPSDCPGTS+GR+RSV KCK+PFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

[Close]

BLAST of Pay0000070 vs. NCBI nr
Match: XP_038875517.1 (tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 628.6 bits (1620), Expect = 3.0e-176
Identity = 303/334 (90.72%), Postives = 314/334 (94.01%), Query Frame = 0


Query: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRG PNAEDSS I  PPFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240
           CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVK          G LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVK----------GPLVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLV 300

Query: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
           SINYKEDHLKLPSDCPGTS+GR+RSV KCK+PFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 324

[Close]

BLAST of Pay0000070 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.6e-119
Identity = 211/328 (64.33%), Postives = 249/328 (75.91%), Query Frame = 0


Query: 8   SPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIAYDGTRYA 67
           +P LPP SL  +  + SP   S ++        DS         ++KWRL+IAYDGTR+A
Sbjct: 5   TPALPP-SLFTNGYLSSPQNISMTNLNSSVKVSDSG--------AYKWRLVIAYDGTRFA 64

Query: 68  GWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVTPFNYDSL 127
           GWQYQ SPPTIQ ++E+ALI  T+L RK+L L+GA RTD GVHAWGQVAHFVTPFNY SL
Sbjct: 65  GWQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSL 124

Query: 128 ESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQRCYTYHCV 187
           +S HAALNGLLP DIRVRE+S A P+FHARFS  SKVY Y+IYN T MDPFQR + YHC 
Sbjct: 125 DSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCA 184

Query: 188 YKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQLEVEGSG 247
           YKLN + M+EAAN FVGKHDFSAF NA   + V +P+K I RFDVI+MG+L+QLEVEGSG
Sbjct: 185 YKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSG 244

Query: 248 FLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYAS-GVPPQGLCLVSINYKE 307
           FLYRQVRNMV LL+QIGKEAL  ++VP IL ++DR+ LAKY S    P GLCLVS+ YKE
Sbjct: 245 FLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKE 304

Query: 308 DHLKLPSDCPGTSLGRSRSVGKCKVPFY 335
           DHLKLP DCP TS GR  ++ KCK+PFY
Sbjct: 305 DHLKLPLDCPVTSSGRHHTITKCKLPFY 323

[Close]

BLAST of Pay0000070 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 81.6 bits (200), Expect = 1.3e-15
Identity = 81/266 (30.45%), Postives = 115/266 (43.23%), Query Frame = 0


Query: 57  LLIAYDGTRYAGWQYQ-RSPPTIQCLIEEALIGATKL--ERKDLHLVGASRTDKGVHAWG 116
           L I Y G R+ G+  + +  P+I+  + +AL     L  ++KD       RTDKGV + G
Sbjct: 94  LKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTG 153

Query: 117 QV------AHFVTPFNYDSLESVHA------------ALNGLLPVDIRVREMSPAAPDFH 176
           QV      +   +P   DS   V+              LN  LP DIRV   SPA  DFH
Sbjct: 154 QVIALFLRSRLKSPPG-DSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFH 213

Query: 177 ARFSTLSKVYHYKIYNGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAA 236
           ARFS  ++ Y Y  +                   LN +AM  A   F+G+HDF  F    
Sbjct: 214 ARFSCYAREYKYFFWR----------------QNLNLSAMDFAGKKFIGEHDFRNFCKMD 273

Query: 237 HNNRVVNPVKVIFRFDVI------EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALP 294
             N      +V F F+V       E   L    + GS FL+ Q+R MV +L  IG+    
Sbjct: 274 VANVHCYTRRVTF-FEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMIGQGVES 333

The following BLAST results are available for this feature:

BLAST of Pay0000070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6MEE7	2.3e-54	41.96	tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=...	[more]
B1I1B5	2.7e-47	43.82	tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477...	[more]
Q8R7Y7	6.1e-47	44.76	tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongen...	[more]
A4J151	1.3e-44	41.04	tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491...	[more]
A3DJK8	2.8e-44	40.16	tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 2...	[more]

BLAST of Pay0000070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7L6	1.1e-195	100.00	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A5D3BY40	3.1e-187	100.00	Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0KB67	9.7e-181	95.12	tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1	[more]
A0A6J1L0L1	3.3e-168	85.03	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]
A0A6J1H621	6.1e-167	84.13	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]

BLAST of Pay0000070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458279.1	2.2e-195	100.00	PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo]	[more]
XP_004138518.2	2.9e-187	96.12	uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical ...	[more]
TYK02989.1	6.5e-187	100.00	protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa]	[more]
XP_038875516.1	6.7e-184	93.41	tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida]	[more]
XP_038875517.1	3.0e-176	90.72	tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida]	[more]

BLAST of Pay0000070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06950.1	3.6e-119	64.33	Pseudouridine synthase family protein	[more]
AT1G34150.1	1.3e-15	30.45	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001406	Pseudouridine synthase I, TruA	TIGRFAM	TIGR00071	TIGR00071	coord: 57..289 e-value: 2.0E-57 score: 192.5
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 48..314
IPR001406	Pseudouridine synthase I, TruA	HAMAP	MF_00171	TruA	coord: 53..304 score: 32.253128
IPR001406	Pseudouridine synthase I, TruA	CDD	cd02570	PseudoU_synth_EcTruA	coord: 57..304 e-value: 1.3133E-109 score: 317.108
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 161..303 e-value: 1.3E-39 score: 137.1
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 60..158 e-value: 9.7E-6 score: 26.0 coord: 198..304 e-value: 3.6E-25 score: 88.5
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 52..160 e-value: 3.5E-29 score: 103.2
None	No IPR available	PIRSR	PIRSR001430-2	PIRSR001430-2	coord: 94..299 e-value: 1.4E-63 score: 212.1
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 48..314
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 53..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0000070.1	Pay0000070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034196	acylglycerol transport
biological_process	GO:1990052	ER to chloroplast lipid transport
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0008033	tRNA processing
biological_process	GO:0009451	RNA modification
molecular_function	GO:0070300	phosphatidic acid binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity