PI0028918 (gene) Melon (PI 482460) v1

Overview
NamePI0028918
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptioneukaryotic translation initiation factor 5-like
Locationchr03: 5139948 .. 5143309 (+)
RNA-Seq ExpressionPI0028918
SyntenyPI0028918
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGTTTGCCCTAATTGGTGACGTGTAGGGTTTTCTTTCTACCATTCCTTTGAGCGTGTATATAACCCCTTTCCTTCTCCCCATCAAAATCCCATCGGCTTCTTCGTCACCGCGAAAACAAGAGCCAAAGGCTTTGAGGATCAGTTTTTTTTTTTCTCCCATCACCACCATTCATCACTGTAAGGCAAGAGTTTTTCTTTGTTTTTCTTGTTTAATCTAACTTCATCACCGTTTCTTGTTGATTTATCGTCCTTGATTTGTTGTTTTTGTTATTGTTCGAATCGATTATTAGTTATTGATGATTGTTTTGTCTCTAATGTCTTTGGTTTAATGAAATCTTTAGGATCGGTACTCCGATTATTGATTTTTGATGGAATTTTGTGAGTTTCTTGCCGTGTTGTTCAGATCTGTAGATTTACTTATTTGTTTGGTATGGTTTTCTTAATTGCATCCTTGATCGGAATGGAAATTATTATCGGCGTTTTAGATTGGTTTTTGTTTATGTAAAGCTGATGAGGACGATGATTGAGTCTTATCTGTGAATTCGATTTTTATTATTCTTTCTATCTAGGATTTTATTTAGGTTTGGGATATTCGTTGTATCCGTTTGTGTTTAATTGTGTATTGCGTTTCGTATTTGCTTATCACTTCGAATTTTTCGATTATCTATTTTCTAGTTGTAAATGATATAGTCTGCGTTTATCTAGTTAGATGTGTGTTAAATGGTGAATGAAGCTTCAATCTAGATTGTTTTTCATTTTGTGTCTGATTGTAAGATTTGGAATGCCTTCCATTTTGTGTATGACTGTAAGATCATTTTCCTCTTCTAGAACTTGCTCGATTTAATTTGAACTGCCGGTCAATGGTTTCACTGGCCTTAGCTGAATTTAAAATTAGTCCATTGAGGTTACGAATAACATATTTGTGCGCCACAATTTGCACTTGAATGATGGTATTTTATGTTGACTGTGAACGTGTTTAAGTTCTGATTATTGATTCTGAAATAATGTTTGTTCTAGATCTTTATTTCCCAATGGTATTTTTCTATTTTCTTGTTATATGTTTCTGTTCAATCATGGTCATAAGGTAATCTTGCTTATTATGTGTTTTTAATCCTCAGGAAGTTGGCCATTTGGTCTGCTGTTCTGTAGTTCAAAGTGGAGGGCGTTACGTTTGAAACTCCTTTTATGTCTGAACGGGCCTCTCTCTTTTTGTCATCGATTGGATTGCTTGGAGAGTATTGTCCTACGTCTAGGAAAAAGATCAGTTGTGCTGAGACTATTGGCTGCATGCATCTTGAGTTGCTTGGAGTTCATAGAAAAGCACGTGGTCTTGGTTTCCATTTTATGCGGCCATTCCCAGCTGCTCAAAGAGCATCAGGACTTTTTGAAGATACTGCCACTTCTACCTATCTTTTCTAACTAGAACTTGCAACAACATGGCGTTGCAGAATATTGGTGCCTCGAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAAGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTTGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACATGAGACAGCAAAGCTGGCTGGTCTTCTCGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCCGAGACTGAGATAATCATCACTAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACATTTATTCTCAAGAATCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATTCGCTGAAAAAGAAAAGCACTACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGATGAAGATGATGACGATGTGCAGTGGCAAACAGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCGACTACCGAAGAGAACAAGTCATCAAAGAAGTCATCCGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGGGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTTCCTCCACAACCGATCTCAAATCATTCTTGGAATCACTCTCCGGAACTCGCCAAGAGATTGTGAACGCCTTAATGGAGGCTCTATTTGATGGCATTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAAAAGAAGAAGTTCCTGGCTGCAGTTGCTGCTGAGGAAGGATCACAGATACTTCTTTTACGTGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCGTTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTACAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCGTTCATCGAATGGCTCCAAAATGCCGAGTCGGAGACGGAAGAAGAGTAAGAATATTACTGCACACTTTTTTGTTATGGTATCTCACTGCCTTTGCGTTGGGTTTTTAATTTTGCTGTCATAGTTTTCTGTTTAGCATTTCTGGAATAAGGGATTATCGTTTGTTCCATCATGTTGTGTTCTCATGTGTCGCGTGTTCTATTTGGGTGTGTTTGGGTTATAATGAACCTTGTCTTTGAACAATGGTATTGTGTTTGTTGTTTCTTCAACTAATAGCGTGTCCCTTATAAACACATGTTTAGAACTGCTGTTGAGTTTTTATTGAACAATGTTATTGCTTTTTCTTGTTTCTTTAGATCTGCTGTTTAGTTTCATTGATCTTTCAGTTTAGTCCATTTTCAATAAGCTTATTTTGCTTCATAAACTAACTTTTAGCATTTTGTTAATTTGACTTTTCATATCCATAATAATTTATCTATGTAATCTTACCGTATAGAAATGAAATTTATGTTTATTGTTTCATTTAATTTTTAAAACTTGGCTTTGTTAAGAGGATGTTTAGAAAATGCATTGGTAAGTCTTGACATAAAATGCATTGGTAAGTTTTGACATTAAAGAATAAACAACGGCTCGTTA

mRNA sequence

AAAAGTTTGCCCTAATTGGTGACGTGTAGGGTTTTCTTTCTACCATTCCTTTGAGCGTGTATATAACCCCTTTCCTTCTCCCCATCAAAATCCCATCGGCTTCTTCGTCACCGCGAAAACAAGAGCCAAAGGCTTTGAGGATCAGTTTTTTTTTTTCTCCCATCACCACCATTCATCACTGAAGTTGGCCATTTGGTCTGCTGTTCTGTAGTTCAAAGTGGAGGGCGTTACGTTTGAAACTCCTTTTATGTCTGAACGGGCCTCTCTCTTTTTGTCATCGATTGGATTGCTTGGAGAGTATTGTCCTACGTCTAGGAAAAAGATCAGTTGTGCTGAGACTATTGGCTGCATGCATCTTGAGTTGCTTGGAGTTCATAGAAAAGCACGTGGTCTTGGTTTCCATTTTATGCGGCCATTCCCAGCTGCTCAAAGAGCATCAGGACTTTTTGAAGATACTGCCACTTCTACCTATCTTTTCTAACTAGAACTTGCAACAACATGGCGTTGCAGAATATTGGTGCCTCGAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAAGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTTGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACATGAGACAGCAAAGCTGGCTGGTCTTCTCGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCCGAGACTGAGATAATCATCACTAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACATTTATTCTCAAGAATCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATTCGCTGAAAAAGAAAAGCACTACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGATGAAGATGATGACGATGTGCAGTGGCAAACAGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCGACTACCGAAGAGAACAAGTCATCAAAGAAGTCATCCGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGGGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTTCCTCCACAACCGATCTCAAATCATTCTTGGAATCACTCTCCGGAACTCGCCAAGAGATTGTGAACGCCTTAATGGAGGCTCTATTTGATGGCATTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAAAAGAAGAAGTTCCTGGCTGCAGTTGCTGCTGAGGAAGGATCACAGATACTTCTTTTACGTGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCGTTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTACAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCGTTCATCGAATGGCTCCAAAATGCCGAGTCGGAGACGGAAGAAGAGTAAGAATATTACTGCACACTTTTTTGTTATGGTATCTCACTGCCTTTGCGTTGGGTTTTTAATTTTGCTGTCATAGTTTTCTGTTTAGCATTTCTGGAATAAGGGATTATCGTTTGTTCCATCATGTTGTGTTCTCATGTGTCGCGTGTTCTATTTGGGTGTGTTTGGGTTATAATGAACCTTGTCTTTGAACAATGGTATTGTGTTTGTTGTTTCTTCAACTAATAGCGTGTCCCTTATAAACACATGTTTAGAACTGCTGTTGAGTTTTTATTGAACAATGTTATTGCTTTTTCTTGTTTCTTTAGATCTGCTGTTTAGTTTCATTGATCTTTCAGTTTAGTCCATTTTCAATAAGCTTATTTTGCTTCATAAACTAACTTTTAGCATTTTGTTAATTTGACTTTTCATATCCATAATAATTTATCTATGTAATCTTACCGTATAGAAATGAAATTTATGTTTATTGTTTCATTTAATTTTTAAAACTTGGCTTTGTTAAGAGGATGTTTAGAAAATGCATTGGTAAGTCTTGACATAAAATGCATTGGTAAGTTTTGACATTAAAGAATAAACAACGGCTCGTTA

Coding sequence (CDS)

ATGGCGTTGCAGAATATTGGTGCCTCGAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAAGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTTGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACATGAGACAGCAAAGCTGGCTGGTCTTCTCGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCCGAGACTGAGATAATCATCACTAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACATTTATTCTCAAGAATCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATTCGCTGAAAAAGAAAAGCACTACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGATGAAGATGATGACGATGTGCAGTGGCAAACAGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCGACTACCGAAGAGAACAAGTCATCAAAGAAGTCATCCGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGGGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTTCCTCCACAACCGATCTCAAATCATTCTTGGAATCACTCTCCGGAACTCGCCAAGAGATTGTGAACGCCTTAATGGAGGCTCTATTTGATGGCATTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAAAAGAAGAAGTTCCTGGCTGCAGTTGCTGCTGAGGAAGGATCACAGATACTTCTTTTACGTGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCGTTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTACAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCGTTCATCGAATGGCTCCAAAATGCCGAGTCGGAGACGGAAGAAGAGTAA

Protein sequence

MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAVAAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEEE
Homology
BLAST of PI0028918 vs. ExPASy Swiss-Prot
Match: P48724 (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 4.6e-159
Identity = 307/449 (68.37%), Postives = 359/449 (79.96%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGA N DDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAK L RPASYTTKYFG
Sbjct: 1   MALQNIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTS VNGAHETAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI 
Sbjct: 61  CELGAQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKV 180

Query: 181 KKDSLKKKSTTTTKDAVTK-VPSKKKNNGSDED-HSPTRSHADDNDRAADDEDDDD--VQ 240
           KK+ +KKK +++ KD   K   SKKK +GSDED  SPT    ++ + A D++DDDD  VQ
Sbjct: 181 KKE-VKKKGSSSAKDGTAKSTISKKKGSGSDEDRRSPTHKQIEEKEEAKDEDDDDDDGVQ 240

Query: 241 WQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTH 300
           W TDTSL+A++QRI+EQLSAVTADMVML+T E  K  K +S     + NGN        +
Sbjct: 241 WLTDTSLDASRQRIKEQLSAVTADMVMLTTDEPEKKKKAASNQNGGSQNGNS-----KNY 300

Query: 301 GALIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKF 360
           G ++ E+K  LKKG   ++L S L +L    QE ++AL+EALF+G  KGF +E +KKK +
Sbjct: 301 GTVVAEVKANLKKGFGASELLSHLAALPVPAQEKMSALVEALFEGTEKGFGRETLKKKNY 360

Query: 361 LAAVAAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVA 420
            AA  AEEGSQILLL AIE FC   +  A KEVALVLK+LYD DV+EEE ++ WYQ+G+ 
Sbjct: 361 FAAAVAEEGSQILLLHAIEEFCCKPNSNALKEVALVLKTLYDADVLEEEAIVLWYQKGLK 420

Query: 421 GANKSSQIWKYVKPFIEWLQNAESETEEE 446
           G NK+S+IWK  +PFI+WLQNAESE++EE
Sbjct: 421 GDNKNSKIWKNAQPFIDWLQNAESESDEE 443

BLAST of PI0028918 vs. ExPASy Swiss-Prot
Match: Q9C8F1 (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX=3702 GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.1e-156
Identity = 305/453 (67.33%), Postives = 360/453 (79.47%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KK++  KK   TT  +  KV  KK       DHSP RS +D+ND+A  +EDDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNG---VSTHG 300
           TS EAA++R++EQLSAVTA+MVMLST EE    K  +E +K+    ++NGN     + H 
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEE---KKPVAEVKKAPEQVHENGNSKIPENAHE 300

Query: 301 ALIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFL 360
            L+ EIK+ L  GSS T LK+ L S S   QE ++AL  ALF G GKGF+KEV+KKKK+L
Sbjct: 301 KLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKKKYL 360

Query: 361 AAV-----AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQ 420
            A+      A   +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ +++WY 
Sbjct: 361 LALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVEWYN 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEEE 446
           +GV    KSS + K V PFIEWLQNAESE+EEE
Sbjct: 421 KGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

BLAST of PI0028918 vs. ExPASy Swiss-Prot
Match: P55876 (Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 9.0e-155
Identity = 308/455 (67.69%), Postives = 359/455 (78.90%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMV+IAKAL RPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG SLVNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI+
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMIS 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKKG KDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKN--NGSDEDH--SPTRSHADDNDRAADDEDDDDVQ 240
           KKD+ KK S    KD+  K   KK     GSDEDH  SPTRSH  D D+AA D+DDDDVQ
Sbjct: 181 KKDAKKKGS----KDSTAKGLKKKATTATGSDEDHSSSPTRSH--DGDKAAADDDDDDVQ 240

Query: 241 WQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNG------ 300
           WQTDTS+EAAKQR+QEQLSA TA+MVMLS TEE +   K   H+  + NG+         
Sbjct: 241 WQTDTSIEAAKQRMQEQLSAATAEMVMLS-TEETEKKMKQPTHKDGSTNGSAKEIPNDKP 300

Query: 301 --NGVSTHGALIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSK 360
                S +  LI +IK  L    + + LK+ L S +   Q+++NA +EALF G+GKGF+K
Sbjct: 301 AVTKPSPYEELIGDIKASLGSAPTPSQLKAVLASSTLPPQDVMNAPLEALFGGVGKGFTK 360

Query: 361 EVVKKKKFLAAVAAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVL 420
           EVVK KK+LA    +EG+Q LL++AIE+F    +PEA KEV +VLK+LYDGD+++EE ++
Sbjct: 361 EVVKNKKYLAVAVPDEGAQTLLVQAIEAFGGKCNPEALKEVPVVLKALYDGDILDEETIV 420

Query: 421 QWYQQGVAGANKSSQIWKYVKPFIEWLQNAESETE 444
            WY   VA A K SQ+ K  KPF+EWLQ+AES+ E
Sbjct: 421 DWYNDAVA-AGKDSQVVKNAKPFVEWLQSAESDEE 447

BLAST of PI0028918 vs. ExPASy Swiss-Prot
Match: Q9S825 (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX=3702 GN=At1g77840 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.7e-153
Identity = 308/450 (68.44%), Postives = 360/450 (80.00%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDD-VQWQT 240
           K    KKK+ +  KD+ T      KN+ SDED SP   H ++     +DEDDDD V+WQT
Sbjct: 181 K---AKKKALSNGKDSKT-----SKNHSSDEDISP--KHDENALEVDEDEDDDDGVEWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTE-ENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           DTS EAA++R+ EQLSA TA+MVMLS  E E K + KS    KS  N  K  N       
Sbjct: 241 DTSREAAEKRMMEQLSAKTAEMVMLSAMEVEEKKAPKS----KSNGNVVKTENPPPQEKN 300

Query: 301 LIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLA 360
           L+Q++K+YLKKGS  + LKSF+ SLS   Q+I++AL  ALFDG+GKGF+KEV KKK +LA
Sbjct: 301 LVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLA 360

Query: 361 AVAA--EEGSQILLLRAIESFC-LNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGV 420
           A A   E+GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL WY++G+
Sbjct: 361 AAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGL 420

Query: 421 AGANKSSQIWKYVKPFIEWLQNAESETEEE 446
            GA+KSS +WK VKPF+EWLQ+AESE+EEE
Sbjct: 421 TGADKSSPVWKNVKPFVEWLQSAESESEEE 436

BLAST of PI0028918 vs. ExPASy Swiss-Prot
Match: Q07205 (Eukaryotic translation initiation factor 5 OS=Rattus norvegicus OX=10116 GN=Eif5 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.1e-46
Identity = 147/448 (32.81%), Postives = 221/448 (49.33%), Query Frame = 0

Query: 13  DAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEK 72
           D FYRYKMP+++ K+EG+GNGIKT +VNMV++AKAL RP +Y TKYFGCELGAQ++FD K
Sbjct: 11  DQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK 70

Query: 73  TGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMITMKCAACGFISD 132
               +VNG+HE  KL  +L+ FIKK+V C  C NPET++ +  K Q I   C ACG+   
Sbjct: 71  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGM 130

Query: 133 VDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDSLKKKSTT 192
           +D   KL TFILKNPPE       KK                 E++KK +K   K+  + 
Sbjct: 131 LDTHHKLCTFILKNPPENSDIGTGKK-----------------EKEKKNRKGKDKENGSV 190

Query: 193 TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDTSLEAAKQRIQ 252
           +T +     P          + SP          A ++E+DDD  W  DT+ EA ++R+ 
Sbjct: 191 STSETPPPPP--------PNEISP--------PHAVEEEEDDD--WGEDTTEEAQRRRMD 250

Query: 253 EQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQEIKDYLKK-- 312
           E              ++  K    S + E++                 +  + D++KK  
Sbjct: 251 E-------------ISDHAKGLTLSDDLERTVEE-------------RVNILFDFVKKKK 310

Query: 313 -----GSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVK-KKKFLAAVAAE 372
                 SS  D+ +  E L       +  L E LFD   +   +++ K ++ FL      
Sbjct: 311 EEGIIDSSDKDIVAEAERLDVKAMGPL-VLTEVLFD---EKIREQIKKYRRHFLRFCHNN 370

Query: 373 EGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQG---VAGANK 432
           + +Q  LL  +E        +   ++  +LK +YD D++EEE ++ W ++          
Sbjct: 371 KKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKEL 393

Query: 433 SSQIWKYVKPFIEWLQNAESET---EEE 446
           + +I    +PFI+WL+ AE E+   EEE
Sbjct: 431 AKEIRVKAEPFIKWLKEAEEESSGGEEE 393

BLAST of PI0028918 vs. ExPASy TrEMBL
Match: A0A0A0LMB4 (W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.0e-235
Identity = 435/445 (97.75%), Postives = 441/445 (99.10%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKS TTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD
Sbjct: 181 KKDVLKKKS-TTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300

Query: 301 QEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAV 360
           QEIKDYLKKG+STTDLKSFLESL+GTRQEIVNAL+EALFDG+GKGFSKEVVKKKKFLA V
Sbjct: 301 QEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360

Query: 361 AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANK 420
           AA EGSQI+LLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVL+WYQQGVAGANK
Sbjct: 361 AAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 446
           SSQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of PI0028918 vs. ExPASy TrEMBL
Match: A0A1S3BCI3 (eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC103488416 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.5e-232
Identity = 430/445 (96.63%), Postives = 439/445 (98.65%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKS TTTKDAVTKVPSKKKNNGSDEDHSPTRSHA DND+AADDEDDDDVQWQTD
Sbjct: 181 KKDVLKKKS-TTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTH +LI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVSLI 300

Query: 301 QEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAV 360
           QEIKD+LKKGSSTTDLKSFLESLSGTRQEIVNAL+EALFDG+GKGFSKEVVKKKKFLA V
Sbjct: 301 QEIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360

Query: 361 AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANK 420
           AAEEGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVL+WY+QGVAGANK
Sbjct: 361 AAEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 446
           SSQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of PI0028918 vs. ExPASy TrEMBL
Match: A0A5A7VCS9 (Eukaryotic translation initiation factor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001250 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.6e-231
Identity = 429/445 (96.40%), Postives = 438/445 (98.43%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKS TTTKDAVTKVPSKKKNNGSDEDHSP RSHA DND AADDEDDDDVQWQTD
Sbjct: 181 KKDVLKKKS-TTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDLAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG+LI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGSLI 300

Query: 301 QEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAV 360
           QEIKD+LKKGSSTTDLKSFLESLSGTRQEIVNAL+EALFDG+GKGFSKEVVKKKKFLA V
Sbjct: 301 QEIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360

Query: 361 AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANK 420
           A+EEGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVL+WY+QGVAGANK
Sbjct: 361 ASEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 446
           SSQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of PI0028918 vs. ExPASy TrEMBL
Match: A0A6J1BQ30 (eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 GN=LOC111004721 PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 3.7e-220
Identity = 409/448 (91.29%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTT-TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAA-DDEDDDDVQWQ 240
           KKDS+KKKST   TKDAVTKVPSKKKNNGSDEDHSPTRS A DND AA DDED+DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS+E+EKS  NGNKNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLA 360
           L+ EIKDYLKKGSS  +LKSFLESLSGTRQE+VNAL+E LFDG+GKGFSKEV+KKK FLA
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFLA 360

Query: 361 AVAAEEG-SQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAG 420
           A AA+EG SQI+LLRAIES CLNT+PEAGKEVALVLKSLYDGDV+EEEFVL+WYQQG+AG
Sbjct: 361 AAAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 446
            NKSSQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ENKSSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of PI0028918 vs. ExPASy TrEMBL
Match: A0A6J1HCN3 (probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3662 GN=LOC111462865 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 400/448 (89.29%), Postives = 422/448 (94.20%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG +LVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTT-TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQT 240
           KKDSLKKKST+   K+AVTKVPSKKK+NGSDEDHSPTRSHA +ND+AADD+DDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS EHEKS  NG+KNGNGVSTHGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300

Query: 301 IQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAA 360
           + EIKDYLKK  S  DLKSFLESL+GT QE  NAL+EALF+G+GKGFSKEVVKKKKFL A
Sbjct: 301 VHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAA--EEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAG 420
           VAA  EEGSQI+LLR+IESFCLNT PE  KE+ALVLKSLYDGDVIEEEFVL+WYQQG+AG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 446
           ANK SQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of PI0028918 vs. NCBI nr
Match: XP_004150695.1 (eukaryotic translation initiation factor 5 [Cucumis sativus] >XP_011649412.1 eukaryotic translation initiation factor 5 [Cucumis sativus] >KAE8652938.1 hypothetical protein Csa_017777 [Cucumis sativus])

HSP 1 Score: 822.8 bits (2124), Expect = 1.4e-234
Identity = 435/445 (97.75%), Postives = 441/445 (99.10%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKS TTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD
Sbjct: 181 KKDVLKKKS-TTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300

Query: 301 QEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAV 360
           QEIKDYLKKG+STTDLKSFLESL+GTRQEIVNAL+EALFDG+GKGFSKEVVKKKKFLA V
Sbjct: 301 QEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360

Query: 361 AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANK 420
           AA EGSQI+LLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVL+WYQQGVAGANK
Sbjct: 361 AAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 446
           SSQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of PI0028918 vs. NCBI nr
Match: XP_008445359.1 (PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo] >XP_008445360.1 PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo])

HSP 1 Score: 812.4 bits (2097), Expect = 2.0e-231
Identity = 430/445 (96.63%), Postives = 439/445 (98.65%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKS TTTKDAVTKVPSKKKNNGSDEDHSPTRSHA DND+AADDEDDDDVQWQTD
Sbjct: 181 KKDVLKKKS-TTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTH +LI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVSLI 300

Query: 301 QEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAV 360
           QEIKD+LKKGSSTTDLKSFLESLSGTRQEIVNAL+EALFDG+GKGFSKEVVKKKKFLA V
Sbjct: 301 QEIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360

Query: 361 AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANK 420
           AAEEGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVL+WY+QGVAGANK
Sbjct: 361 AAEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 446
           SSQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of PI0028918 vs. NCBI nr
Match: KAA0064817.1 (eukaryotic translation initiation factor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 811.6 bits (2095), Expect = 3.3e-231
Identity = 429/445 (96.40%), Postives = 438/445 (98.43%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKS TTTKDAVTKVPSKKKNNGSDEDHSP RSHA DND AADDEDDDDVQWQTD
Sbjct: 181 KKDVLKKKS-TTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDLAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG+LI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGSLI 300

Query: 301 QEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAV 360
           QEIKD+LKKGSSTTDLKSFLESLSGTRQEIVNAL+EALFDG+GKGFSKEVVKKKKFLA V
Sbjct: 301 QEIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360

Query: 361 AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANK 420
           A+EEGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVL+WY+QGVAGANK
Sbjct: 361 ASEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 446
           SSQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of PI0028918 vs. NCBI nr
Match: XP_038884689.1 (eukaryotic translation initiation factor 5-like [Benincasa hispida])

HSP 1 Score: 807.0 bits (2083), Expect = 8.2e-230
Identity = 423/445 (95.06%), Postives = 434/445 (97.53%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKA+RRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKALRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKDSLKKKST TTKDAVTKVPSKKKNNGSDEDHSP RSHA DND+AADD+DDDDVQWQTD
Sbjct: 181 KKDSLKKKSTGTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDQAADDDDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENK SKKS EHEKSA NGNKNGNGVSTHGALI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKLSKKSPEHEKSAVNGNKNGNGVSTHGALI 300

Query: 301 QEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLAAV 360
           QEIKDYLKKG S  DLKSFLESLSGTRQEIVNAL+EALFDG+GKGFSKEVVKKKKFLA+ 
Sbjct: 301 QEIKDYLKKGPSAADLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLASA 360

Query: 361 AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAGANK 420
           AAEEGSQI+LLRAIESFC+NTSPEAGKEVALVLKSLYDGDVI+EEFVL+WYQQGVAGANK
Sbjct: 361 AAEEGSQIILLRAIESFCINTSPEAGKEVALVLKSLYDGDVIDEEFVLEWYQQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 446
           SS IWK+VKPFIEWLQNAESETEEE
Sbjct: 421 SSHIWKHVKPFIEWLQNAESETEEE 445

BLAST of PI0028918 vs. NCBI nr
Match: XP_022131574.1 (eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131575.1 eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131576.1 eukaryotic translation initiation factor 5-like [Momordica charantia])

HSP 1 Score: 773.9 bits (1997), Expect = 7.7e-220
Identity = 409/448 (91.29%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTT-TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAA-DDEDDDDVQWQ 240
           KKDS+KKKST   TKDAVTKVPSKKKNNGSDEDHSPTRS A DND AA DDED+DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS+E+EKS  NGNKNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLA 360
           L+ EIKDYLKKGSS  +LKSFLESLSGTRQE+VNAL+E LFDG+GKGFSKEV+KKK FLA
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFLA 360

Query: 361 AVAAEEG-SQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGVAG 420
           A AA+EG SQI+LLRAIES CLNT+PEAGKEVALVLKSLYDGDV+EEEFVL+WYQQG+AG
Sbjct: 361 AAAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 446
            NKSSQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ENKSSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of PI0028918 vs. TAIR 10
Match: AT1G36730.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 553.5 bits (1425), Expect = 1.5e-157
Identity = 305/453 (67.33%), Postives = 360/453 (79.47%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KK++  KK   TT  +  KV  KK       DHSP RS +D+ND+A  +EDDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNG---VSTHG 300
           TS EAA++R++EQLSAVTA+MVMLST EE    K  +E +K+    ++NGN     + H 
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEE---KKPVAEVKKAPEQVHENGNSKIPENAHE 300

Query: 301 ALIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFL 360
            L+ EIK+ L  GSS T LK+ L S S   QE ++AL  ALF G GKGF+KEV+KKKK+L
Sbjct: 301 KLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKKKYL 360

Query: 361 AAV-----AAEEGSQILLLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQ 420
            A+      A   +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ +++WY 
Sbjct: 361 LALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVEWYN 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEEE 446
           +GV    KSS + K V PFIEWLQNAESE+EEE
Sbjct: 421 KGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

BLAST of PI0028918 vs. TAIR 10
Match: AT1G77840.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 543.9 bits (1400), Expect = 1.2e-154
Identity = 308/450 (68.44%), Postives = 360/450 (80.00%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDD-VQWQT 240
           K    KKK+ +  KD+ T      KN+ SDED SP   H ++     +DEDDDD V+WQT
Sbjct: 181 K---AKKKALSNGKDSKT-----SKNHSSDEDISP--KHDENALEVDEDEDDDDGVEWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTE-ENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           DTS EAA++R+ EQLSA TA+MVMLS  E E K + KS    KS  N  K  N       
Sbjct: 241 DTSREAAEKRMMEQLSAKTAEMVMLSAMEVEEKKAPKS----KSNGNVVKTENPPPQEKN 300

Query: 301 LIQEIKDYLKKGSSTTDLKSFLESLSGTRQEIVNALMEALFDGIGKGFSKEVVKKKKFLA 360
           L+Q++K+YLKKGS  + LKSF+ SLS   Q+I++AL  ALFDG+GKGF+KEV KKK +LA
Sbjct: 301 LVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLA 360

Query: 361 AVAA--EEGSQILLLRAIESFC-LNTSPEAGKEVALVLKSLYDGDVIEEEFVLQWYQQGV 420
           A A   E+GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL WY++G+
Sbjct: 361 AAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGL 420

Query: 421 AGANKSSQIWKYVKPFIEWLQNAESETEEE 446
            GA+KSS +WK VKPF+EWLQ+AESE+EEE
Sbjct: 421 TGADKSSPVWKNVKPFVEWLQSAESESEEE 436

BLAST of PI0028918 vs. TAIR 10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0

Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

BLAST of PI0028918 vs. TAIR 10
Match: AT5G20920.2 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0

Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 156 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 215

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 216 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 246

BLAST of PI0028918 vs. TAIR 10
Match: AT5G20920.3 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0

Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P487244.6e-15968.37Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5... [more]
Q9C8F12.1e-15667.33Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX... [more]
P558769.0e-15567.69Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1[more]
Q9S8251.7e-15368.44Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX... [more]
Q072053.1e-4632.81Eukaryotic translation initiation factor 5 OS=Rattus norvegicus OX=10116 GN=Eif5... [more]
Match NameE-valueIdentityDescription
A0A0A0LMB47.0e-23597.75W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=... [more]
A0A1S3BCI39.5e-23296.63eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7VCS91.6e-23196.40Eukaryotic translation initiation factor 5-like OS=Cucumis melo var. makuwa OX=1... [more]
A0A6J1BQ303.7e-22091.29eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 G... [more]
A0A6J1HCN31.2e-21589.29probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
XP_004150695.11.4e-23497.75eukaryotic translation initiation factor 5 [Cucumis sativus] >XP_011649412.1 euk... [more]
XP_008445359.12.0e-23196.63PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo] >XP_00... [more]
KAA0064817.13.3e-23196.40eukaryotic translation initiation factor 5-like [Cucumis melo var. makuwa][more]
XP_038884689.18.2e-23095.06eukaryotic translation initiation factor 5-like [Benincasa hispida][more]
XP_022131574.17.7e-22091.29eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131... [more]
Match NameE-valueIdentityDescription
AT1G36730.11.5e-15767.33Translation initiation factor IF2/IF5 [more]
AT1G77840.11.2e-15468.44Translation initiation factor IF2/IF5 [more]
AT5G20920.13.8e-0729.03eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.23.8e-0729.03eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.33.8e-0729.03eukaryotic translation initiation factor 2 beta subunit [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 159..179
NoneNo IPR availableGENE3D2.20.25.350coord: 105..145
e-value: 1.6E-19
score: 71.2
NoneNo IPR availableGENE3D3.30.30.170coord: 2..104
e-value: 9.0E-43
score: 147.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..224
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..294
NoneNo IPR availablePANTHERPTHR23001EUKARYOTIC TRANSLATION INITIATION FACTORcoord: 1..445
NoneNo IPR availablePANTHERPTHR23001:SF28EUKARYOTIC TRANSLATION INITIATION FACTOR 5-1-RELATEDcoord: 1..445
NoneNo IPR availableCDDcd11561W2_eIF5coord: 294..443
e-value: 2.61163E-37
score: 131.584
IPR003307W2 domainSMARTSM00515542_3coord: 354..441
e-value: 2.6E-31
score: 120.0
IPR003307W2 domainPFAMPF02020W2coord: 367..445
e-value: 6.6E-20
score: 71.1
IPR003307W2 domainPROSITEPS51363W2coord: 289..445
score: 24.667263
IPR002735Translation initiation factor IF2/IF5SMARTSM00653eIF2Bneu4coord: 15..129
e-value: 9.2E-63
score: 224.5
IPR002735Translation initiation factor IF2/IF5PFAMPF01873eIF-5_eIF-2Bcoord: 11..127
e-value: 6.1E-37
score: 126.1
IPR016021MIF4G-like domain superfamilyGENE3D1.25.40.180coord: 293..436
e-value: 9.8E-29
score: 102.4
IPR016190Translation initiation factor IF2/IF5, zinc-bindingSUPERFAMILY75689Zinc-binding domain of translation initiation factor 2 betacoord: 98..133
IPR016189Translation initiation factor IF2/IF5, N-terminalSUPERFAMILY100966Translation initiation factor 2 beta, aIF2beta, N-terminal domaincoord: 10..101
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 298..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028918.1PI0028918.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001732 formation of cytoplasmic translation initiation complex
biological_process GO:0001731 formation of translation preinitiation complex
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0006413 translational initiation
cellular_component GO:0005829 cytosol
molecular_function GO:0071074 eukaryotic initiation factor eIF2 binding
molecular_function GO:0005092 GDP-dissociation inhibitor activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0005515 protein binding