PI0028848 (gene) Melon (PI 482460) v1

Overview
NamePI0028848
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionenoyl-CoA hydratase/isomerase A
Locationchr11: 3712451 .. 3715562 (-)
RNA-Seq ExpressionPI0028848
SyntenyPI0028848
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGTAAGCAGATAGAATTGAAAGTGTTTTTCAGTCTTTATCTAACATGAAAGATAAGATTTCAACGTTTCCCATGATTTGCTCGTTTTTCAATACGCAATTATCCACAAACTTCCCCATACCTTTCTCAATTTCAATGCGGCAACTCTTTTTAATTCAATCTTCATTACCCAATTTCATCATTTCTTCCCCGGAATGGATCGTTCTTCGCCGGAAAATCTCATCCTCGTCAAAAGAGAATCCAATGGGATCGCATTCATTACAATCAACCGCCCGAAGTCGTTGAATTCATTAACGAGACCGATGATGACAAATTTGGCTCAGGCGTTTAAGAGCTTAGACCGCGACGATTCCGTCCGGGTCATCATCCTCTCTGGATCAGGAAGAGCCTTCTGCTCCGGTGTAGATCTAACTGCGGCGGAGGATGTTTTCAAGGGAGATGTGAAGGATGTTGAATCCGATCCGGTTGCGCAAATGGAACTCTGCCGGAAGCCGATCATCGGAGCGATTGCAGGTTTTGCTATCACAGCTGGATTTGAGATCGCTTTGGCTTGTGACATACTCATCGCTGCCAAAGGAGCCAAGTTCATCGACACTCATGCTAGGTTTTTTCCACTTTCTTTCAATTAATTGATTAATTTGCTAGGATTAGGGGAAACAAAGTAAATTTCTGAAATCTTTTGGCGATGCGATGCTGTTCTTTTATTGAAATTTCTGGAATCAAATACTGTTATGACAGATTTGGGATATTTCCTTCATGGGGATTGTCTCAGAAATTAGGAAGGATAATTGGAGTCAACAAAGCTCGTGAAGTATCGTTAACGGCGATGCCTCTAACGGCGGAGGAAGCAGAGAAGAGGGGATTGGTGAATCAGGTGGTTGAAGGAAGTGAATTGTTGAAGAAAGCTCGAGCGGTTGCGGAGGCAATCTTGAAGAACAATCAGGATTTGGTAGTCAGATACAAGTCAGTCATTAACGATGGCTTGAAGCTTGATCTTGGCCAAGCTCTTGCGCTGGAAAAGGTGATTCATAACTATTTCGAATCTTTATTCATCATTGAATTCTGATTTCTTGTTCATGAATTTTGATTCAAATTTGTCTTGAATGCAAGGATGTGGACTGAAAAATTGATCGAAATGGATTAATTTCAACTAAAATTTGTTTGGAATTACACACTCAGAACTCCCAAAAATTTATACAGAACTGTTAAAATTGGACTCTCCTAGGAATTAGACCCTCAATGATGAACATTGAATTCTCTAGAATTGTTAACCTTAAGCCTTCAAACTTGTAAGCTAGTAGAAATTTTTGTCTATTGAACAATTAATTTTATAGGACTGTTATTATGTTCTTTTAGATTCATATATTTGGGCAATGGGATGGGATGGTCCTTTCAGTACTCATTTTAAGTTTTTTCAATTTTTGAAGTAGATGTTAGTAAGGGTCGAATTTTGTTACATAACTAGATAGTAATATTAAGTATATTAGTATTAAGGAATACCATTAAATGGAAAGTTAGTCACGGGATGTTGTATAAAATAGCACTCAAGGGAGAGGGTTCTTAGTACCATAAACTTGGATTTATACTGTAGTTCTTCTTTAATTCTCAATATATTTCAGATATTGATTCTTGTTAGTTAGTATCCTAATAAACTTCTTTCACAAATTTTATCTATTTTATCATTTAGGAGACGGTTGAACCATTGAATTGGTTATTGACTTGTTACCGTTGCTAGTTACAATAGTCTATAGGTTATAATAAACTATGTTTAGGATGCAAACCATTTTAGGTTGGGGTAGAAAATAATGTAATGAAGAAAGAAAAAGATAACAAATAGTAAATAATAATGTAACAAATAGTTTAGGCTGTAGTTAAAGAGGGATTTGAAGAAAGAAAAAGATAACGAATAGCAAATAGTAAATAATAACCTAACAAATAGTATAGGTTGTAGTTAAAGAAGGATTTGAATAGTAATATTGTAGTTAATGGTAAGTTATAATAGTTAGAAACAACAATTTGAAGTTCATGACTCAAACACCTCCGAAATGTTTGAGATTCAATCTCTACGATTATGTAAGTTTAAATGAGCAACTTAAACATTTGATAAGAGCTCAATTTTTATCGTTAGAGAGACATTTCGGACAAAGGAGTTGATTATTATAATCTTAGGTTATTGTTGTTGAGTTTAGTTGTGTTTGGGACATAAATTATTTTAGTTTGAATTATAATTATATGTGTCTGAAGTGTAAACTATTTTGACTCGAGAAATAAATAATAAACTTTGTAACAAAGAATAAACATGTAATAAACACTGTAGCAAATAGAAGTTTTGAAATAGTATTGATGTAGCTCATTGGAAAGTACAAAATAGTATGGTAGTGTAGCAAGAGGTGGCTATTATAGTCTTAGGATAACCCTTGTTCCAAACGATCCCTAAACGTTTGAGTGTATAATTGTAGTTAACATCACACTTCATAGTATTTTTTGCAAAGAAAAAAAAAATAATCAAATAGTTATCGAAGTAATAGTCGTTAAATGTGGCATATTAAACTCAAGTTTATGTCCAAAAGTTGGTACTTAGTAATTGAGTGTTTGTGTTTTGTGAATACAGGAGAGAGCTCATGCGTATTACAATGGAATGACGAAAGAACAATTTCAGAAAATGCAAGAATTCATAGCTGGTCGAAGCTCCAAGAGACCTTCCAAGTTATAAGTCTAACCCCTTTCTCTTTTTCAATAATAACAAAAACAAACCCTTCATCTGTGTTTTTTAGCTGTTGCTATATTCAAAACAAGAATTTCATCTTCATACCCTCGTACCACTTCATAAACAATCTCATCTTAGTAGTTCTAAAATACATAGTTCCATATATAAAGTCCAAGAAGAATTCATTCTAATTCTAACCTTTTAGCTTTACTTATCAAACTCTAACTTTTTCTAGATCAAATTTATAAATTATTAGTTTAAAATACTATTTTGGTTTTTAGATCCTTGTTTAATTTTAGAAAATTTCAATAAATTTTAAATTTAGTTCATTTAGTTTTGTTGATTTTTCAAAATTGTTCAATTAACCCTTGAGTTATTTAGGAATGGATTGTAGAGTTTTGTG

mRNA sequence

ATTTGTAAGCAGATAGAATTGAAAGTGTTTTTCAGTCTTTATCTAACATGAAAGATAAGATTTCAACGTTTCCCATGATTTGCTCGTTTTTCAATACGCAATTATCCACAAACTTCCCCATACCTTTCTCAATTTCAATGCGGCAACTCTTTTTAATTCAATCTTCATTACCCAATTTCATCATTTCTTCCCCGGAATGGATCGTTCTTCGCCGGAAAATCTCATCCTCGTCAAAAGAGAATCCAATGGGATCGCATTCATTACAATCAACCGCCCGAAGTCGTTGAATTCATTAACGAGACCGATGATGACAAATTTGGCTCAGGCGTTTAAGAGCTTAGACCGCGACGATTCCGTCCGGGTCATCATCCTCTCTGGATCAGGAAGAGCCTTCTGCTCCGGTGTAGATCTAACTGCGGCGGAGGATGTTTTCAAGGGAGATGTGAAGGATGTTGAATCCGATCCGGTTGCGCAAATGGAACTCTGCCGGAAGCCGATCATCGGAGCGATTGCAGGTTTTGCTATCACAGCTGGATTTGAGATCGCTTTGGCTTGTGACATACTCATCGCTGCCAAAGGAGCCAAGTTCATCGACACTCATGCTAGATTTGGGATATTTCCTTCATGGGGATTGTCTCAGAAATTAGGAAGGATAATTGGAGTCAACAAAGCTCGTGAAGTATCGTTAACGGCGATGCCTCTAACGGCGGAGGAAGCAGAGAAGAGGGGATTGGTGAATCAGGTGGTTGAAGGAAGTGAATTGTTGAAGAAAGCTCGAGCGGTTGCGGAGGCAATCTTGAAGAACAATCAGGATTTGGTAGTCAGATACAAGTCAGTCATTAACGATGGCTTGAAGCTTGATCTTGGCCAAGCTCTTGCGCTGGAAAAGGAGAGAGCTCATGCGTATTACAATGGAATGACGAAAGAACAATTTCAGAAAATGCAAGAATTCATAGCTGGTCGAAGCTCCAAGAGACCTTCCAAGTTATAAGTCTAACCCCTTTCTCTTTTTCAATAATAACAAAAACAAACCCTTCATCTGTGTTTTTTAGCTGTTGCTATATTCAAAACAAGAATTTCATCTTCATACCCTCGTACCACTTCATAAACAATCTCATCTTAGTAGTTCTAAAATACATAGTTCCATATATAAAGTCCAAGAAGAATTCATTCTAATTCTAACCTTTTAGCTTTACTTATCAAACTCTAACTTTTTCTAGATCAAATTTATAAATTATTAGTTTAAAATACTATTTTGGTTTTTAGATCCTTGTTTAATTTTAGAAAATTTCAATAAATTTTAAATTTAGTTCATTTAGTTTTGTTGATTTTTCAAAATTGTTCAATTAACCCTTGAGTTATTTAGGAATGGATTGTAGAGTTTTGTG

Coding sequence (CDS)

ATGGATCGTTCTTCGCCGGAAAATCTCATCCTCGTCAAAAGAGAATCCAATGGGATCGCATTCATTACAATCAACCGCCCGAAGTCGTTGAATTCATTAACGAGACCGATGATGACAAATTTGGCTCAGGCGTTTAAGAGCTTAGACCGCGACGATTCCGTCCGGGTCATCATCCTCTCTGGATCAGGAAGAGCCTTCTGCTCCGGTGTAGATCTAACTGCGGCGGAGGATGTTTTCAAGGGAGATGTGAAGGATGTTGAATCCGATCCGGTTGCGCAAATGGAACTCTGCCGGAAGCCGATCATCGGAGCGATTGCAGGTTTTGCTATCACAGCTGGATTTGAGATCGCTTTGGCTTGTGACATACTCATCGCTGCCAAAGGAGCCAAGTTCATCGACACTCATGCTAGATTTGGGATATTTCCTTCATGGGGATTGTCTCAGAAATTAGGAAGGATAATTGGAGTCAACAAAGCTCGTGAAGTATCGTTAACGGCGATGCCTCTAACGGCGGAGGAAGCAGAGAAGAGGGGATTGGTGAATCAGGTGGTTGAAGGAAGTGAATTGTTGAAGAAAGCTCGAGCGGTTGCGGAGGCAATCTTGAAGAACAATCAGGATTTGGTAGTCAGATACAAGTCAGTCATTAACGATGGCTTGAAGCTTGATCTTGGCCAAGCTCTTGCGCTGGAAAAGGAGAGAGCTCATGCGTATTACAATGGAATGACGAAAGAACAATTTCAGAAAATGCAAGAATTCATAGCTGGTCGAAGCTCCAAGAGACCTTCCAAGTTATAA

Protein sequence

MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILSGSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNGMTKEQFQKMQEFIAGRSSKRPSKL
Homology
BLAST of PI0028848 vs. ExPASy Swiss-Prot
Match: Q6NL24 (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECHIA PE=1 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-109
Identity = 203/262 (77.48%), Postives = 229/262 (87.40%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MD++  ENLI VK+ES GIA ITINRPKSLNSLTR MM +LA+AFK +D D+SV+V+I +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E+DPV QME  RKPIIGAI GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DIL+A++GAKF+DTHARFGIFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A K G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N VVE  E LKKAR +AEAI+KN Q +V+R KSVINDGLKLDLG AL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPS 263
           MTKEQF+KMQEFIAGR SK+PS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of PI0028848 vs. ExPASy Swiss-Prot
Match: P52046 (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=crt PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.4e-33
Identity = 88/253 (34.78%), Postives = 148/253 (58.50%), Query Frame = 0

Query: 8   NLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILSGSG-RAF 67
           N +++++E   +A +TINRPK+LN+L    +  +      ++ D  V  +IL+G+G ++F
Sbjct: 4   NNVILEKEGK-VAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSF 63

Query: 68  CSGVDLTAAEDV--FKGDVKDVESDPV-AQMELCRKPIIGAIAGFAITAGFEIALACDIL 127
            +G D++  +++   +G    +  + V  ++EL  KP+I A+ GFA+  G EIA++CDI 
Sbjct: 64  VAGADISEMKEMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIR 123

Query: 128 IAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQV 187
           IA+  A+F       GI P +G +Q+L R++G+  A+++  TA  + A+EA + GLVN+V
Sbjct: 124 IASSNARFGQPEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKV 183

Query: 188 VEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNGMTK 247
           VE SEL+  A+ +A  I+ N    V   K  IN G++ D+  ALA E E     ++  T+
Sbjct: 184 VEPSELMNTAKEIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS--TE 243

Query: 248 EQFQKMQEFIAGR 257
           +Q   M  FI  R
Sbjct: 244 DQKDAMTAFIEKR 253

BLAST of PI0028848 vs. ExPASy Swiss-Prot
Match: P64017 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA8 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 6.8e-33
Identity = 87/249 (34.94%), Postives = 135/249 (54.22%), Query Frame = 0

Query: 10  ILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILSGSGRAFCSG 69
           ILV+R+   +  IT+NRP++LN+L   +M  +  A   LD D  +  II++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVESDPV-AQMELCRKPIIGAIAGFAITAGFEIALACDILIAAKG 129
            D+    D+   D    +      ++   R P I A+AG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSE 189
           AKF     + G+ P  G SQ+L R IG  KA ++ LT   + A EAE+ GLV++VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNGMTKEQFQK 249
           LL +ARA A  I + +       K  +N   +  L + L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

BLAST of PI0028848 vs. ExPASy Swiss-Prot
Match: P9WNN8 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=echA8 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 6.8e-33
Identity = 87/249 (34.94%), Postives = 135/249 (54.22%), Query Frame = 0

Query: 10  ILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILSGSGRAFCSG 69
           ILV+R+   +  IT+NRP++LN+L   +M  +  A   LD D  +  II++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVESDPV-AQMELCRKPIIGAIAGFAITAGFEIALACDILIAAKG 129
            D+    D+   D    +      ++   R P I A+AG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSE 189
           AKF     + G+ P  G SQ+L R IG  KA ++ LT   + A EAE+ GLV++VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNGMTKEQFQK 249
           LL +ARA A  I + +       K  +N   +  L + L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

BLAST of PI0028848 vs. ExPASy Swiss-Prot
Match: P9WNN9 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=echA8 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 6.8e-33
Identity = 87/249 (34.94%), Postives = 135/249 (54.22%), Query Frame = 0

Query: 10  ILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILSGSGRAFCSG 69
           ILV+R+   +  IT+NRP++LN+L   +M  +  A   LD D  +  II++GS +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVESDPV-AQMELCRKPIIGAIAGFAITAGFEIALACDILIAAKG 129
            D+    D+   D    +      ++   R P I A+AG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSE 189
           AKF     + G+ P  G SQ+L R IG  KA ++ LT   + A EAE+ GLV++VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNGMTKEQFQK 249
           LL +ARA A  I + +       K  +N   +  L + L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

BLAST of PI0028848 vs. ExPASy TrEMBL
Match: A0A1S3C8X4 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497788 PE=4 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.4e-136
Identity = 258/264 (97.73%), Postives = 261/264 (98.86%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSSPENLILV RESNGIAFITINRPKSLNSLTRPMM +LA+AFKSLDRDDSVRVIILS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           NQVVEGSELLKKAR VAEAILKNNQDLV+RYKSVINDGLKLDLGQALALEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQFQKMQEFIAGRSSKRPSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of PI0028848 vs. ExPASy TrEMBL
Match: A0A0A0KCM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330970 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 9.2e-134
Identity = 255/264 (96.59%), Postives = 257/264 (97.35%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSS ENLILV RESNGIAFITINRPKSLNSLT+ MM +LA AFKSLDRDDSVRVIILS
Sbjct: 1   MDRSSSENLILVNRESNGIAFITINRPKSLNSLTKHMMADLAHAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           NQVVEGSELLKKAR VAEAILKNNQDLVVRYKSVINDGLKLDLGQAL LEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVVRYKSVINDGLKLDLGQALTLEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQFQKMQEFIAGRSSKRPSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of PI0028848 vs. ExPASy TrEMBL
Match: A0A6J1H7Z0 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111460389 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 8.4e-127
Identity = 236/264 (89.39%), Postives = 251/264 (95.08%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSSPE LILV RE NGIAF+TINRPKSLNSLTR MM +LAQAFK+LDRD+SVRVI+LS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N VV+G ELLKK R +AEAILKNNQDLV+RYKSVINDGLKLDLG ALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of PI0028848 vs. ExPASy TrEMBL
Match: A0A6J1L4H0 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.4e-126
Identity = 236/264 (89.39%), Postives = 250/264 (94.70%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSS E LILV RE NGIAF+TINRPKSLNSLTR MM +LAQAFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N VV+G ELLKK R +AEAILKNNQDLV+RYKSVINDGLKLDLG ALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of PI0028848 vs. ExPASy TrEMBL
Match: A0A6J1L1R6 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.6e-125
Identity = 235/264 (89.02%), Postives = 249/264 (94.32%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSS E LILV RE NGIAF+TINRPKSLNSLTR MM +LAQAFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N VV+G ELLKK R +AEAILKNNQDLV+RYKSVINDGLKLDLG ALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of PI0028848 vs. NCBI nr
Match: XP_008458352.1 (PREDICTED: probable enoyl-CoA hydratase 1, peroxisomal [Cucumis melo])

HSP 1 Score: 494.2 bits (1271), Expect = 7.0e-136
Identity = 258/264 (97.73%), Postives = 261/264 (98.86%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSSPENLILV RESNGIAFITINRPKSLNSLTRPMM +LA+AFKSLDRDDSVRVIILS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           NQVVEGSELLKKAR VAEAILKNNQDLV+RYKSVINDGLKLDLGQALALEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQFQKMQEFIAGRSSKRPSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of PI0028848 vs. NCBI nr
Match: XP_004148801.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucumis sativus] >KGN47460.1 hypothetical protein Csa_021779 [Cucumis sativus])

HSP 1 Score: 486.1 bits (1250), Expect = 1.9e-133
Identity = 255/264 (96.59%), Postives = 257/264 (97.35%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSS ENLILV RESNGIAFITINRPKSLNSLT+ MM +LA AFKSLDRDDSVRVIILS
Sbjct: 1   MDRSSSENLILVNRESNGIAFITINRPKSLNSLTKHMMADLAHAFKSLDRDDSVRVIILS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           NQVVEGSELLKKAR VAEAILKNNQDLVVRYKSVINDGLKLDLGQAL LEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVVRYKSVINDGLKLDLGQALTLEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQFQKMQEFIAGRSSKRPSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of PI0028848 vs. NCBI nr
Match: XP_038876166.1 (probable enoyl-CoA hydratase 1, peroxisomal [Benincasa hispida])

HSP 1 Score: 482.3 bits (1240), Expect = 2.8e-132
Identity = 249/264 (94.32%), Postives = 257/264 (97.35%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSSPENLILV RESNGIAF+TINRPKSLNSLTR MMT+LAQAFKSLDRDDSVRVI+LS
Sbjct: 1   MDRSSPENLILVTRESNGIAFVTINRPKSLNSLTRSMMTDLAQAFKSLDRDDSVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVESDPV QME CRKPIIGAIAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVVQMERCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGA FIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKR LV
Sbjct: 121 DILIAAKGAMFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRNLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N+VVEG ELLKKARA+AEAILKNNQD+V+RYKSVINDGLKLDLGQALALEKERAHAYYNG
Sbjct: 181 NEVVEGGELLKKARAIAEAILKNNQDMVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQFQKMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKKPSKL 264

BLAST of PI0028848 vs. NCBI nr
Match: XP_022959404.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 463.0 bits (1190), Expect = 1.7e-126
Identity = 236/264 (89.39%), Postives = 251/264 (95.08%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSSPE LILV RE NGIAF+TINRPKSLNSLTR MM +LAQAFK+LDRD+SVRVI+LS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N VV+G ELLKK R +AEAILKNNQDLV+RYKSVINDGLKLDLG ALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of PI0028848 vs. NCBI nr
Match: KAG6575117.1 (putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461.1 bits (1185), Expect = 6.6e-126
Identity = 236/264 (89.39%), Postives = 250/264 (94.70%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MDRSSPE LILV RE NGIAF+TINRPKSLNSLTR MM +LAQAFK+LDRD+SVRVI+LS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DILIAAKGAKFIDTHARFGIFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N VV+G ELLKK R +AEAILKNNQDLV+RYKSVINDGLKLDLG ALALEKERAHAYY G
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYTG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of PI0028848 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 397.1 bits (1019), Expect = 1.1e-110
Identity = 203/262 (77.48%), Postives = 229/262 (87.40%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILS 60
           MD++  ENLI VK+ES GIA ITINRPKSLNSLTR MM +LA+AFK +D D+SV+V+I +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120
           GSGR+FCSGVDLTAAE VFKGDVKD E+DPV QME  RKPIIGAI GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180
           DIL+A++GAKF+DTHARFGIFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A K G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NQVVEGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLDLGQALALEKERAHAYYNG 240
           N VVE  E LKKAR +AEAI+KN Q +V+R KSVINDGLKLDLG AL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFQKMQEFIAGRSSKRPS 263
           MTKEQF+KMQEFIAGR SK+PS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of PI0028848 vs. TAIR 10
Match: AT1G60550.1 (enoyl-CoA hydratase/isomerase D )

HSP 1 Score: 110.5 bits (275), Expect = 2.0e-24
Identity = 77/214 (35.98%), Postives = 107/214 (50.00%), Query Frame = 0

Query: 8   NLILVKRESNGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILSGSG-RAF 67
           ++I  K    GIA ITINRP+  N+     +  L +AF     D SV VIIL+G G +AF
Sbjct: 76  DIIYEKALDEGIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAF 135

Query: 68  CSGVDLTAAEDVFKGDVKDVES----DPVAQMELCRKPIIGAIAGFAITAGFEIALACDI 127
           CSG D          D  DV      D   Q+    KP+I  +AG+A+  G  + + CD+
Sbjct: 136 CSGGDQALRTQDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDL 195

Query: 128 LIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQ 187
            IAA  A F  T  + G F +   S  + R++G  KARE+       TA EAEK GL+N 
Sbjct: 196 TIAADNAIFGQTGPKVGSFDAGYGSSIMSRLVGPKKAREMWFMTRFYTASEAEKMGLINT 255

Query: 188 VVEGSELLKKARAVAEAILKNNQDLVVRYKSVIN 217
           VV   +L K+       IL+N+   +   K+ +N
Sbjct: 256 VVPLEDLEKETVKWCREILRNSPTAIRVLKAALN 289

BLAST of PI0028848 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 105.9 bits (263), Expect = 5.0e-23
Identity = 75/245 (30.61%), Postives = 120/245 (48.98%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAF-ITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIIL 60
           M   S + L ++++ ++   F + INRP  LN+L+        +A  SLD++  V VIIL
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGSGRAFCSGVDLTAAEDV----------------FKGDVKDVESDPVAQMELCRKPIIG 120
           SG+G+ FCSG+DL +   +                 +  +K +++  +  +E CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AIAGFAITAGFEIALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVS 180
           AI G  I  G ++  ACDI   ++ A F        I    G  Q+L  I+G   A E++
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELA 180

Query: 181 LTAMPLTAEEAEKRGLVNQVV-EGSELLKKARAVAEAILKNNQDLVVRYKSVINDGLKLD 228
           LTA   +  EA+  GLV++V    SEL      +AE I   +   V   K+V+    ++ 
Sbjct: 181 LTARRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVS 240

BLAST of PI0028848 vs. TAIR 10
Match: AT3G06860.1 (multifunctional protein 2 )

HSP 1 Score: 104.8 bits (260), Expect = 1.1e-22
Identity = 70/220 (31.82%), Postives = 121/220 (55.00%), Query Frame = 0

Query: 17  NGIAFITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIILSGSGRAFCSGVDLTAAE 76
           +G+A IT+  P  +NSL+  ++ NL   ++     + V+ I+++G+   F  G D++   
Sbjct: 16  DGVAVITLINP-PVNSLSFDVLYNLKSNYEEALSRNDVKAIVITGAKGRFSGGFDISGFG 75

Query: 77  DVFKGDVKDVESDPVA------QMELCRKPIIGAIAGFAITAGFEIALACDILIAAKGAK 136
           ++ KG+VK+ ++  ++       +E  RKP + AI G A+  G E+A+AC   I+A  A+
Sbjct: 76  EMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLELAMACHARISAPAAQ 135

Query: 137 FIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLVNQVVEGSELL 196
                 + G+ P +G +Q+L R++G+ KA E+ LT+ P+ AEE    GL++ VV  +EL+
Sbjct: 136 LGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHSLGLIDAVVPPAELV 195

Query: 197 KKARAVAEAILKNNQDLVVRYKSVINDGLKLD----LGQA 227
             AR  A        D+V R K  ++   K D    LG+A
Sbjct: 196 TTARRWA-------LDIVGRRKPWVSSVSKTDKLPPLGEA 227

BLAST of PI0028848 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 103.6 bits (257), Expect = 2.5e-22
Identity = 69/216 (31.94%), Postives = 108/216 (50.00%), Query Frame = 0

Query: 1   MDRSSPENLILVKRESNGIAF-ITINRPKSLNSLTRPMMTNLAQAFKSLDRDDSVRVIIL 60
           M   S + L ++++ ++   F + INRP  LN+L+        +A  SLD++  V VIIL
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGSGRAFCSGVDLTAAEDV----------------FKGDVKDVESDPVAQMELCRKPIIG 120
           SG+G+ FCSG+DL +   +                 +  +K +++  +  +E CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AIAGFAITAGFEIALACDILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVS 180
           AI G  I  G ++  ACDI   ++ A F        I    G  Q+L  I+G   A E++
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELA 180

Query: 181 LTAMPLTAEEAEKRGLVNQVV-EGSELLKKARAVAE 199
           LTA   +  EA+  GLV++V    SEL      +AE
Sbjct: 181 LTARRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAE 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NL241.5e-10977.48Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=E... [more]
P520461.4e-3334.78Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /... [more]
P640176.8e-3334.94Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /... [more]
P9WNN86.8e-3334.94Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain CDC 155... [more]
P9WNN96.8e-3334.94Probable enoyl-CoA hydratase echA8 OS=Mycobacterium tuberculosis (strain ATCC 25... [more]
Match NameE-valueIdentityDescription
A0A1S3C8X43.4e-13697.73probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497... [more]
A0A0A0KCM09.2e-13496.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330970 PE=4 SV=1[more]
A0A6J1H7Z08.4e-12789.39probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1L4H05.4e-12689.39probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1L1R64.6e-12589.02probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_008458352.17.0e-13697.73PREDICTED: probable enoyl-CoA hydratase 1, peroxisomal [Cucumis melo][more]
XP_004148801.11.9e-13396.59probable enoyl-CoA hydratase 1, peroxisomal [Cucumis sativus] >KGN47460.1 hypoth... [more]
XP_038876166.12.8e-13294.32probable enoyl-CoA hydratase 1, peroxisomal [Benincasa hispida][more]
XP_022959404.11.7e-12689.39probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata][more]
KAG6575117.16.6e-12689.39putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT4G16210.11.1e-11077.48enoyl-CoA hydratase/isomerase A [more]
AT1G60550.12.0e-2435.98enoyl-CoA hydratase/isomerase D [more]
AT5G43280.15.0e-2330.61delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT3G06860.11.1e-2231.82multifunctional protein 2 [more]
AT5G43280.22.5e-2231.94delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.226.10coord: 3..239
e-value: 1.6E-67
score: 229.2
NoneNo IPR availablePANTHERPTHR43802ENOYL-COA HYDRATASEcoord: 5..260
NoneNo IPR availableCDDcd06558crotonase-likecoord: 10..200
e-value: 2.7558E-67
score: 205.102
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 15..240
e-value: 1.7E-54
score: 184.8
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 8..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028848.1PI0028848.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009536 plastid
molecular_function GO:0003824 catalytic activity