PI0028787 (gene) Melon (PI 482460) v1

Overview
NamePI0028787
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr01: 25568986 .. 25570752 (-)
RNA-Seq ExpressionPI0028787
SyntenyPI0028787
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAGTTTTATGTTGTTTTCTGCAATTTGGTTATCTTTTGTTTCTTGTCTTCTTTCTTCTTCTCAAGCTAGATGGCATCATCACACTAAGCAAAAACACTCTCATCACCATAGACATAGCTACATTTCACACCCGCCTACTCTTTCTCCTTCTCCTTCGGATTCACCGGACGATGGAGATTATATCAATGATACGAATATTTTCGACGTGAGAGCTTTTGGTGCTGTTGGGGATGGCATTGTAGACGATACGGAGGCATTCAAGATGGCTTGGGACACGGCATGTCAGAGTGAGGATGACTCTGCAGTCATGCTTGTTCCCTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGCCATGGTTACCTCATGTTACGAGTTGATGGCACGTTGATGCCACCGGACGGACCCGATGCTTGGCCGAAGAATGCTAGTCGTCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATGTCCTTAGAAGGAGGGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACATAAGGTATATTCAATCTTTTTCCATAAATTTCATTACTAATATATCCTGACACGAGATATTTTGTAAAACTTAGGGGCCAAATGGAACTACATTACCAGGACCGTGTGACAGCCCAATTGTAAGTTCATATATACACTCAAAATTATACTGTACTTTTTTCTGCTATAAGAAATATGAAAAAATTACTTAGCATCACATTCTAATGATTTTAGGCAATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCAAGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGATTGTTCATATCAACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAATACAGACGGAATCCATATCGAAAACACAAATGGCGTCGAGATATATAATTCAGTCATTAACAATGGTTAGTAAGTTGAACAAAAATAAGAATTAGCTTGATAATCACTTTGTTAAAGGAAATGAATTTTAAATCATGCCTATTTATCATTATTTGTAGGGGATGATTGTGTATCAATTGGATCTGGTTCATATGACTTAGATATCAGAAACATCACTTGTGGACCAAGTCATGGAATCAGGTATTGTGTTAATTAGTCGTATTAGTTATGTTGCCAGTTTGAATATATACCAGCTCAACTAGACATTAGTAATTAGTTCTTAAACGTTTTCCTTTCTCTTTCTAATCCAGTATCGGAAGTCTCGGTGTTCGCAACTCGAGAGCTTGTGTTACGAACATCACAGTTAGGGACTCGGTAATCAAAGACTCAGACAATGGAGTTAGGATCAAGACATGGCAAGGTGGATATGGAGCAGTAAGAGAAATAACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGATCAATTCTATTGCTTAACAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGATATTCAATACACAAACATCAAAGGAACATATGACATTCGAAGCCCGCCTATGCACTTTGCTTGCAGTGACACCGTCCCGTGTACCAATCTAACGTTGTCGGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACCCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTTAACAATGACAATGAGTATTGTTGA

mRNA sequence

ATGTCTAGTTTTATGTTGTTTTCTGCAATTTGGTTATCTTTTGTTTCTTGTCTTCTTTCTTCTTCTCAAGCTAGATGGCATCATCACACTAAGCAAAAACACTCTCATCACCATAGACATAGCTACATTTCACACCCGCCTACTCTTTCTCCTTCTCCTTCGGATTCACCGGACGATGGAGATTATATCAATGATACGAATATTTTCGACGTGAGAGCTTTTGGTGCTGTTGGGGATGGCATTGTAGACGATACGGAGGCATTCAAGATGGCTTGGGACACGGCATGTCAGAGTGAGGATGACTCTGCAGTCATGCTTGTTCCCTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGCCATGGTTACCTCATGTTACGAGTTGATGGCACGTTGATGCCACCGGACGGACCCGATGCTTGGCCGAAGAATGCTAGTCGTCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACATAAGGGGCCAAATGGAACTACATTACCAGGACCGTGTGACAGCCCAATTGCAATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCAAGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGATTGTTCATATCAACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAATACAGACGGAATCCATATCGAAAACACAAATGGCGTCGAGATATATAATTCAGTCATTAACAATGGGGATGATTGTGTATCAATTGGATCTGGTTCATATGACTTAGATATCAGAAACATCACTTGTGGACCAAGTCATGGAATCAGTATCGGAAGTCTCGGTGTTCGCAACTCGAGAGCTTGTGTTACGAACATCACAGTTAGGGACTCGGTAATCAAAGACTCAGACAATGGAGTTAGGATCAAGACATGGCAAGGTGGATATGGAGCAGTAAGAGAAATAACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGATCAATTCTATTGCTTAACAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGATATTCAATACACAAACATCAAAGGAACATATGACATTCGAAGCCCGCCTATGCACTTTGCTTGCAGTGACACCGTCCCGTGTACCAATCTAACGTTGTCGGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACCCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTTAACAATGACAATGAGTATTGTTGA

Coding sequence (CDS)

ATGTCTAGTTTTATGTTGTTTTCTGCAATTTGGTTATCTTTTGTTTCTTGTCTTCTTTCTTCTTCTCAAGCTAGATGGCATCATCACACTAAGCAAAAACACTCTCATCACCATAGACATAGCTACATTTCACACCCGCCTACTCTTTCTCCTTCTCCTTCGGATTCACCGGACGATGGAGATTATATCAATGATACGAATATTTTCGACGTGAGAGCTTTTGGTGCTGTTGGGGATGGCATTGTAGACGATACGGAGGCATTCAAGATGGCTTGGGACACGGCATGTCAGAGTGAGGATGACTCTGCAGTCATGCTTGTTCCCTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGCCATGGTTACCTCATGTTACGAGTTGATGGCACGTTGATGCCACCGGACGGACCCGATGCTTGGCCGAAGAATGCTAGTCGTCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACATAAGGGGCCAAATGGAACTACATTACCAGGACCGTGTGACAGCCCAATTGCAATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCAAGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGATTGTTCATATCAACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAATACAGACGGAATCCATATCGAAAACACAAATGGCGTCGAGATATATAATTCAGTCATTAACAATGGGGATGATTGTGTATCAATTGGATCTGGTTCATATGACTTAGATATCAGAAACATCACTTGTGGACCAAGTCATGGAATCAGTATCGGAAGTCTCGGTGTTCGCAACTCGAGAGCTTGTGTTACGAACATCACAGTTAGGGACTCGGTAATCAAAGACTCAGACAATGGAGTTAGGATCAAGACATGGCAAGGTGGATATGGAGCAGTAAGAGAAATAACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGATCAATTCTATTGCTTAACAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGATATTCAATACACAAACATCAAAGGAACATATGACATTCGAAGCCCGCCTATGCACTTTGCTTGCAGTGACACCGTCCCGTGTACCAATCTAACGTTGTCGGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACCCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTTAACAATGACAATGAGTATTGTTGA

Protein sequence

MSSFMLFSAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDDGDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGIGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC
Homology
BLAST of PI0028787 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.1e-155
Identity = 262/442 (59.28%), Postives = 329/442 (74.43%), Query Frame = 0

Query: 22  SQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSD--------SPDDGDYINDTN-IFDVR 81
           ++AR+H+H K +H   H       PP  + +P D        SPD     +D+  +FDV 
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVT 84

Query: 82  AFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIFTGPCHGYLMLRV 141
           +FGAVGDG  DDT AF+ AW  AC  E  S V+L P G  F I STIF+GPC   L+ ++
Sbjct: 85  SFGAVGDGSCDDTAAFQDAWKAACAVE--SGVVLAPEGGVFKITSTIFSGPCKPGLVFQL 144

Query: 142 DGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWWDLPCKPHKGPNGT 201
           DG LMPPDGP+ WP+  ++ QWLVFYR+DG            GQKWWDLPCKPH+GP+G+
Sbjct: 145 DGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGS 204

Query: 202 TLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPGLSPNTD 261
           +  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V IN I I SP LSPNTD
Sbjct: 205 SSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTD 264

Query: 262 GIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRNSRACV 321
           GIH+ NT  V IYNSV++NGDDC+SIG+G  D+DI+ +TCGPSHGISIGSLGV NS+ACV
Sbjct: 265 GIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACV 324

Query: 322 TNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYCLTKDCTN 381
           +NITVR++VI+DSDNG+R+KTWQGG G+V  + F NI M+NV N II+DQ+YC +KDC N
Sbjct: 325 SNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRN 384

Query: 382 QTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQGDIVLDPFCW 441
           +TSAV V D++Y NIKGTYD+RSPP+HFACSDTV CTN+T+SE+ELLP +G++V DPFCW
Sbjct: 385 ETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCW 444

Query: 442 NAYGDLQTLTIPPVSCLQEGLP 444
           NAYG  +TLTIPP+ CL +G P
Sbjct: 445 NAYGKQETLTIPPIDCLLDGSP 463

BLAST of PI0028787 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.7e-87
Identity = 163/386 (42.23%), Postives = 231/386 (59.84%), Query Frame = 0

Query: 68  IFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIFTGPCHGY 127
           +  V  FGA G+G+ DDT+AF  AW TAC S+  + + LVP  ++ +++    +GPC   
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRI-LVPENYTCLLRPIDLSGPCKAR 111

Query: 128 LMLRVDGTLMPPDGPDAWPKNASRRQWLVFY-----------RIDGIGQKWWDLPCKPHK 187
           L L++ GT++ P+ PD W +  +RR+WL F+            ++G+GQ+WW+  CK H 
Sbjct: 112 LTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HN 171

Query: 188 GPNGTTLPGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPG 247
             N      PC  +P A+ F    N+ V+ L + +S Q H     C+ V I+ + + +P 
Sbjct: 172 HSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPA 231

Query: 248 LSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVR 307
            SPNTDGIHI  + G+ I N+ ++ GDDC+SI   S  + I NI CGP HGISIGSLG  
Sbjct: 232 TSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKS 291

Query: 308 NSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYC- 367
            S   V +ITV  ++I D+ NGVRIKTWQGG G V +I F NI M+NV NPIIIDQ+YC 
Sbjct: 292 KSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCD 351

Query: 368 LTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQGDI 427
             K C NQTSA+S+ +I + +++GT       +  +CSD+ PC N+ L +I+L P+ GD 
Sbjct: 352 SRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDG 411

Query: 428 VLDPFCWNAYGDLQTLTIPPVSCLQE 441
             + FCW AYG       PP  CL +
Sbjct: 412 FTESFCWEAYGSSSGQVYPP-PCLSD 426

BLAST of PI0028787 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 7.0e-78
Identity = 156/360 (43.33%), Postives = 208/360 (57.78%), Query Frame = 0

Query: 67  NIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIFTGPCHG 126
           NIF+V  +GAVGDG  D TEAF  AW  AC+    SA++LVP    F++ +  F GPC  
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKK--PSAMLLVPGNKKFVVNNLFFNGPCQP 116

Query: 127 YLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYR-----------IDGIGQKWWDLPCKPH 186
           +   +VDG +     P +W  N   R WL F +           IDG G++WW   C   
Sbjct: 117 HFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC--- 176

Query: 187 KGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPG 246
           K  NG  +    D P AI+F  S+ L +QGLK+ NSP+FH  F  C+ V I  I I +P 
Sbjct: 177 KWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPR 236

Query: 247 LSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVR 306
            SPNTDGI I  +    +  + I  GDDCV+IG+GS ++ I ++ CGP HGISIGSLG  
Sbjct: 237 DSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRE 296

Query: 307 NSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYCL 366
           NSRA V+ + V  +   D+ NG+RIKTWQGG G    I + N+ M N  NPI+I+QFYC 
Sbjct: 297 NSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCT 356

Query: 367 TKD-CTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQGDI 415
           +   C NQ SAV + D+ Y NI+GT    +  +   CSD++PC ++ LS+I L    G I
Sbjct: 357 SASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of PI0028787 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.7e-76
Identity = 155/357 (43.42%), Postives = 210/357 (58.82%), Query Frame = 0

Query: 68  IFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIFTGPCHGY 127
           +F+V  +GAVGDG  D TEAF   W+ AC+    SAV+LVP    F + + +F GPC  +
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKA--SAVLLVPANKKFFVNNLVFRGPCQPH 117

Query: 128 LMLRVDGTLMPPDGPDAWPKNASRRQWLVFYR-----------IDGIGQKWWDLPCKPHK 187
           L  +VDGT++    P  W KN+  + WL F +           IDG GQ+WW   CK   
Sbjct: 118 LSFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV-- 177

Query: 188 GPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPGL 247
             NG T+    + P AI+   S ++TV+ L + NSP+FH  F  C+ V I  + IK+P  
Sbjct: 178 -VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRD 237

Query: 248 SPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRN 307
           SPNTDGI I  +    I   VI  GDDC++IG+GS ++ I+++ CGP HGISIGSLG  N
Sbjct: 238 SPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDN 297

Query: 308 SRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYCLT 367
           SRA V+++ V  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC +
Sbjct: 298 SRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTS 357

Query: 368 KD-CTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQG 413
              C NQ SAV +  + Y NI GT    +  +   CSD+VPCT + LS + L    G
Sbjct: 358 ASACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

BLAST of PI0028787 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.8e-73
Identity = 160/411 (38.93%), Postives = 223/411 (54.26%), Query Frame = 0

Query: 18  LLSSSQARWHHHTK--QKHSHHHRHSYISHPPTLSPSPSDSPDDGDYINDTNIFDVRAFG 77
           +L S   ++H  ++  +K  HH RH                           +F+V  +G
Sbjct: 30  MLDSDTKQYHRSSRNLRKRVHHARHDV-----------------------AIVFNVEHYG 89

Query: 78  AVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIFTGPCHGYLMLRVDGT 137
           AVGDG  D T+AF+  W+ AC     SAV LVP    F++ + +F GPC  +   +VDGT
Sbjct: 90  AVGDGKHDSTDAFEKTWNAACNKL--SAVFLVPANKKFVVNNLVFYGPCQPHFSFKVDGT 149

Query: 138 LMPPDGPDAWPKNASRRQWLVFYR-----------IDGIGQKWWDLPCKPHKGPNGTTLP 197
           +     P  W KN+  + W+ F R           IDG G +WW   CK     NG T+ 
Sbjct: 150 IAAYPDPAKW-KNS--KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKT---INGRTVC 209

Query: 198 GPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPGLSPNTDGIH 257
                P AI+   S ++TV+ L +TNSP+FH  F  C  V I  I IK+P  SPNTDGI 
Sbjct: 210 NDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSPNTDGID 269

Query: 258 IENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRNSRACVTNI 317
           I  +   EI    I  GDDCV++G+GS ++ I+++TCGP HG+SIGSLG  NSR+ V+ +
Sbjct: 270 IFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSRSEVSFV 329

Query: 318 TVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYCLT-KDCTNQT 377
            +  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC +   C NQ 
Sbjct: 330 HLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSAAACKNQR 389

Query: 378 SAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQGDI 415
           SAV + D+ + NI GT    +  +   CSD+VPC+N+ LS + L    G +
Sbjct: 390 SAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

BLAST of PI0028787 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 2.4e-267
Identity = 444/467 (95.07%), Postives = 451/467 (96.57%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           GDYINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of PI0028787 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 7.1e-267
Identity = 443/467 (94.86%), Postives = 450/467 (96.36%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRH YISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           GDYINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of PI0028787 vs. ExPASy TrEMBL
Match: A0A0A0KBA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 7.4e-264
Identity = 439/467 (94.00%), Postives = 447/467 (95.72%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSH HRHSYISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           G YINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDS VMLVPYGFSFMIQSTI
Sbjct: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGV+IYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+NFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of PI0028787 vs. ExPASy TrEMBL
Match: A0A5A7URQ1 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00080 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.3e-254
Identity = 429/467 (91.86%), Postives = 436/467 (93.36%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           GDYINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHK               AIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHK---------------AIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 452

BLAST of PI0028787 vs. ExPASy TrEMBL
Match: A0A6J1JNI5 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.0e-244
Identity = 407/466 (87.34%), Postives = 427/466 (91.63%), Query Frame = 0

Query: 1   MSSFMLFSAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDDG 60
           MS+F+L  + W++F+ CLL SSQARWHHH+KQKHSHHH+ SYISHPPT    PS SP DG
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPT----PSPSPGDG 60

Query: 61  DYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIF 120
           DYIN+TNIFDVR FGAVGDG+ DDTEAFKMAWDTACQS DD+AV+LVPYG+SFMIQSTIF
Sbjct: 61  DYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQS-DDAAVVLVPYGYSFMIQSTIF 120

Query: 121 TGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWWD 180
           TGPCHG+LML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDG+           GQKWWD
Sbjct: 121 TGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWD 180

Query: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSI 240
           LPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCK VHI SI
Sbjct: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESI 240

Query: 241 HIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISI 300
           HIKSPGLSPNTDGIHIENTN V IYNS+I NGDDCVSIGSGSYD+DIRNITCGPSHGISI
Sbjct: 241 HIKSPGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300

Query: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIII 360
           GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPIII
Sbjct: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 360

Query: 361 DQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLP 420
           DQFYCLTKDCTNQTSAVS+SDI+YTNIKGTYDIRS PMH ACSD VPCTNLTLSEIELLP
Sbjct: 361 DQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLP 420

Query: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI  NNDNEYC
Sbjct: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of PI0028787 vs. NCBI nr
Match: TYJ96898.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 930.6 bits (2404), Expect = 5.0e-267
Identity = 444/467 (95.07%), Postives = 451/467 (96.57%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           GDYINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of PI0028787 vs. NCBI nr
Match: XP_008461394.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo])

HSP 1 Score: 929.1 bits (2400), Expect = 1.5e-266
Identity = 443/467 (94.86%), Postives = 450/467 (96.36%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRH YISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           GDYINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of PI0028787 vs. NCBI nr
Match: XP_004135975.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein Csa_016395 [Cucumis sativus])

HSP 1 Score: 919.1 bits (2374), Expect = 1.5e-263
Identity = 439/467 (94.00%), Postives = 447/467 (95.72%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSH HRHSYISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           G YINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDS VMLVPYGFSFMIQSTI
Sbjct: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGV+IYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+NFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of PI0028787 vs. NCBI nr
Match: XP_038899064.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 429/470 (91.28%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MSSFMLFSAIWLSFVSCLL-SSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDS--- 60
           MSSF+L  AIW SF+SCLL SSSQARWHHHTKQKHSHHH+HSYISHPP+LSPSPS S   
Sbjct: 1   MSSFVLSFAIWFSFISCLLSSSSQARWHHHTKQKHSHHHKHSYISHPPSLSPSPSPSDSP 60

Query: 61  PDDGDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQ 120
           PDDGDYIN+TNIFDVRAFGA+G+G+VDDTEAFKMAWDTACQS DDSA+MLVPYG+SFMIQ
Sbjct: 61  PDDGDYINETNIFDVRAFGAIGNGVVDDTEAFKMAWDTACQS-DDSAIMLVPYGYSFMIQ 120

Query: 121 STIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQ 180
           STIFTGPCHG+LMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDG+           GQ
Sbjct: 121 STIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQ 180

Query: 181 KWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVH 240
           KWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK VH
Sbjct: 181 KWWDLPCKPHKGRNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVH 240

Query: 241 INSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSH 300
           I+SIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYDLDIRNITCGPSH
Sbjct: 241 IDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDLDIRNITCGPSH 300

Query: 301 GISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRN 360
           GISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRN
Sbjct: 301 GISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRN 360

Query: 361 PIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEI 420
           PIIIDQFYCLTKDC NQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEI
Sbjct: 361 PIIIDQFYCLTKDCINQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEI 420

Query: 421 ELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           ELLP+QGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NNDNEYC
Sbjct: 421 ELLPSQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 469

BLAST of PI0028787 vs. NCBI nr
Match: KAA0058542.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-253
Identity = 429/467 (91.86%), Postives = 436/467 (93.36%), Query Frame = 0

Query: 1   MSSFMLFS-AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSDSPDD 60
           MSSF+L S AIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPP+LSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120
           GDYINDTNIFDVRAFGA+GDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWW 180
           FTGPCHGYLMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDG+           GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINS 240
           DLPCKPHK               AIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHI+S
Sbjct: 181 DLPCKPHK---------------AIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVR ITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSD+VPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 452

BLAST of PI0028787 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 628.2 bits (1619), Expect = 5.0e-180
Identity = 306/467 (65.52%), Postives = 365/467 (78.16%), Query Frame = 0

Query: 11  WLSFVSCLLSSSQARWHHH-TKQKHSHHHRH--------SYISHPPTLSPSPSDSP---- 70
           +++ +  L+S S+AR HHH  K KH++H+ H        S IS PPT  P P DSP    
Sbjct: 14  FMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSL 73

Query: 71  ---DDGDYINDTN-IFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDS-AVMLVPYGFS 130
                 D  +D N I++VR +GAVGDG  DDTEAFK AWD++C +E+++ +V+LVPYG++
Sbjct: 74  PPSPSDDPADDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYT 133

Query: 131 FMIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYR-----------ID 190
           FMIQSTIFTGPC  Y   +VDGT++ PDGP++WP N S+RQWLVFYR           ID
Sbjct: 134 FMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVID 193

Query: 191 GIGQKWWDLPCKPHKGPNGTTL-PGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 250
           G GQKWWDLPCKPH+  N + +  GPCDSPIA+RFFMSSNL V+GL+I NSPQFHFRFDG
Sbjct: 194 GRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDG 253

Query: 251 CKIVHINSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNIT 310
           C+ VH+ S+HI +P LSPNTDGIHIEN+N V IYNS+I+NGDDCVSIGSGSYD+DIRN+T
Sbjct: 254 CQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLT 313

Query: 311 CGP-SHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIH 370
           CGP  HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V  +TF+NIH
Sbjct: 314 CGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIH 373

Query: 371 MDNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTN 430
           +D+VRNPIIIDQ+YC+TKDC N+TSAV VSDI Y  IKGTYDIRSPPMHF CSD VPCTN
Sbjct: 374 VDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTN 433

Query: 431 LTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI 447
           LTLS+IELLPA+G+IVLDPFCWNAYG  + L+IPPV CL    P  +
Sbjct: 434 LTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

BLAST of PI0028787 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 562.0 bits (1447), Expect = 4.4e-160
Identity = 270/449 (60.13%), Postives = 331/449 (73.72%), Query Frame = 0

Query: 16  SCLLSSSQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSD---SPDDGDYINDTNIFDVR 75
           S L   ++AR HH   Q             PP+ SP+P+D   SP     ++   ++DVR
Sbjct: 22  SLLSYGTEARLHHQASQ-------------PPSPSPNPNDPSKSPSRSQDLDHEVVYDVR 81

Query: 76  AFGAVGDGIVDDTEAFKMAWDTACQS--EDDSAVMLVPYGFSFMIQSTIFTGPCHGYLML 135
            +GAVG+G+ DDT +FK AWD+AC +   + ++V+ VPYGF+FMI+STIFTGPC  Y   
Sbjct: 82  KYGAVGNGVADDTVSFKTAWDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYF 141

Query: 136 RVDGTLMPPDGPDAWPKNASRRQWLVFYR-----------IDGIGQKWWDLPCKPH-KGP 195
           +VDGT++P DGP +WP + ++RQWL FYR           IDG GQ WWDLPCKPH +  
Sbjct: 142 QVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNV 201

Query: 196 NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPGLSP 255
           N T L GPC+SP A+RFFMSSN+ V+GL I NSPQ H + DGC +VHINS+ I SP  SP
Sbjct: 202 NKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASP 261

Query: 256 NTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGP-SHGISIGSLGVRNS 315
           NTDGIHIEN+N VEIYNSVI+NGDDCVSIG G+YD+DIRNITCGP  HGISIGSLG +NS
Sbjct: 262 NTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNS 321

Query: 316 RACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYCLTK 375
            ACV+N+TVRDS IK S+NGVRIKTWQGG G+V  +TF NIH+D VRNPIIIDQ+YC TK
Sbjct: 322 HACVSNVTVRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTK 381

Query: 376 DCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQGDIVLD 435
            C N+TSAV V+DI Y +IKGTYDIRSPPMHF CS+ VPCTNLTLS IELLP++ DIV+ 
Sbjct: 382 SCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVG 441

Query: 436 PFCWNAYGDLQTLTIPPVSCLQEGLPSSI 447
           PFCWNAYG     ++P +SCL+    +S+
Sbjct: 442 PFCWNAYGITDEFSVPLISCLKSNPSTSL 457

BLAST of PI0028787 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 7.7e-157
Identity = 262/442 (59.28%), Postives = 329/442 (74.43%), Query Frame = 0

Query: 22  SQARWHHHTKQKHSHHHRHSYISHPPTLSPSPSD--------SPDDGDYINDTN-IFDVR 81
           ++AR+H+H K +H   H       PP  + +P D        SPD     +D+  +FDV 
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVT 84

Query: 82  AFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTIFTGPCHGYLMLRV 141
           +FGAVGDG  DDT AF+ AW  AC  E  S V+L P G  F I STIF+GPC   L+ ++
Sbjct: 85  SFGAVGDGSCDDTAAFQDAWKAACAVE--SGVVLAPEGGVFKITSTIFSGPCKPGLVFQL 144

Query: 142 DGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-----------GQKWWDLPCKPHKGPNGT 201
           DG LMPPDGP+ WP+  ++ QWLVFYR+DG            GQKWWDLPCKPH+GP+G+
Sbjct: 145 DGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGS 204

Query: 202 TLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHINSIHIKSPGLSPNTD 261
           +  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V IN I I SP LSPNTD
Sbjct: 205 SSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTD 264

Query: 262 GIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRNSRACV 321
           GIH+ NT  V IYNSV++NGDDC+SIG+G  D+DI+ +TCGPSHGISIGSLGV NS+ACV
Sbjct: 265 GIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACV 324

Query: 322 TNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNPIIIDQFYCLTKDCTN 381
           +NITVR++VI+DSDNG+R+KTWQGG G+V  + F NI M+NV N II+DQ+YC +KDC N
Sbjct: 325 SNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRN 384

Query: 382 QTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIELLPAQGDIVLDPFCW 441
           +TSAV V D++Y NIKGTYD+RSPP+HFACSDTV CTN+T+SE+ELLP +G++V DPFCW
Sbjct: 385 ETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCW 444

Query: 442 NAYGDLQTLTIPPVSCLQEGLP 444
           NAYG  +TLTIPP+ CL +G P
Sbjct: 445 NAYGKQETLTIPPIDCLLDGSP 463

BLAST of PI0028787 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.8e-150
Identity = 247/409 (60.39%), Postives = 315/409 (77.02%), Query Frame = 0

Query: 49  LSPSPSDSP--DDGDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSE-DDSAVM 108
           LSPSP+ +P  +D D I  T +FDV +FGA+GD   DDT AFKMAWD AC S    SA++
Sbjct: 27  LSPSPAPNPAYNDNDNIAPT-VFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALL 86

Query: 109 LVPYGFSFMIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGI-- 168
           LVPY F F+++ T F GPC   L+L++DG ++ PDGP +WP N  +RQW++FYR++G+  
Sbjct: 87  LVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYRVNGLSI 146

Query: 169 ---------GQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQF 228
                    GQKWW+LPCKPHKG NGTT  GPCDSP+AIR F SS + +QG+   NS QF
Sbjct: 147 QGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQF 206

Query: 229 HFRFDGCKIVHINSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDL 288
           H RFD C  V ++S+ IK+P  SPNTDGIHIENT+ V+I NS+I+NGDDC+SIG+G +++
Sbjct: 207 HVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNV 266

Query: 289 DIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREIT 348
           DI+N+TCGPSHGISIGSLGV NS+A V+NITV +S I +SDNGVRIKTWQGG G+V  I 
Sbjct: 267 DIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIV 326

Query: 349 FSNIHMDNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDT 408
           FSNI M NVRNPI+IDQ+YC T +C NQTSAV +SD+ Y NIKGTYD+RSPP+HF CSD+
Sbjct: 327 FSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDS 386

Query: 409 VPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLP 444
           VPCTNLTL+E++L P++G  + +PFCWNAYG ++ +T+PPV CL +  P
Sbjct: 387 VPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of PI0028787 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 350.1 bits (897), Expect = 2.6e-96
Identity = 179/410 (43.66%), Postives = 251/410 (61.22%), Query Frame = 0

Query: 59  DGDYINDTNIFDVRAFGAVGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQST 118
           +G      N+ D    GA GDG  DDT+AF+ AW  AC+    ++ +LVP G +F++   
Sbjct: 44  EGGSSGTINVLD---HGAKGDGTSDDTKAFEDAWQVACKVA--ASTLLVPSGSTFLVGPV 103

Query: 119 IFTG-PCHGYLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYR-----------IDGIGQK 178
            F G  C   ++ +++G ++ P    AW   +   QW+ F             IDG G  
Sbjct: 104 SFLGKECKEKIVFQLEGKIIAPTSASAW--GSGLLQWIEFKALQGITIKGKGIIDGRGSV 163

Query: 179 WWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKIVHI 238
           WW+          GT +P     P A+RF+ S+ +TV G+ I NSPQ H +FD C  + +
Sbjct: 164 WWN-------DMMGTKMPR--TKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQV 223

Query: 239 NSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHG 298
           +     SPG SPNTDGIH++N+    IY S +  GDDC+SI +G  +++I ++ CGP HG
Sbjct: 224 SDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHG 283

Query: 299 ISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVREITFSNIHMDNVRNP 358
           ISIG LG  N++ACV+NITVRD  + ++ NGVRIK+WQGG G+V+++ FSNI + NV NP
Sbjct: 284 ISIGGLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANP 343

Query: 359 IIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDTVPCTNLTLSEIE 418
           IIIDQ+YC    C N+TSAV+VS+I Y NIKGTY     P+ FACSD++PCT ++LS IE
Sbjct: 344 IIIDQYYCDGGGCHNETSAVAVSNINYINIKGTY--TKEPVRFACSDSLPCTGISLSTIE 403

Query: 419 LLPAQGDI-VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 456
           L PA G    LDPFCW A+G+L+T T+PP+ CL+          ++N+ C
Sbjct: 404 LKPATGKASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z11.1e-15559.28Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ53.7e-8742.23Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432127.0e-7843.33Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E71.7e-7643.42Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY191.8e-7338.93Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BFC72.4e-26795.07Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00... [more]
A0A1S3CFX87.1e-26794.86polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV... [more]
A0A0A0KBA77.4e-26494.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1[more]
A0A5A7URQ15.3e-25491.86Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00... [more]
A0A6J1JNI51.0e-24487.34polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
TYJ96898.15.0e-26795.07polygalacturonase [Cucumis melo var. makuwa][more]
XP_008461394.11.5e-26694.86PREDICTED: polygalacturonase At1g48100-like [Cucumis melo][more]
XP_004135975.11.5e-26394.00polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein C... [more]
XP_038899064.11.5e-25591.28polygalacturonase At1g48100-like [Benincasa hispida][more]
KAA0058542.11.1e-25391.86polygalacturonase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G02460.15.0e-18065.52Pectin lyase-like superfamily protein [more]
AT4G01890.14.4e-16060.13Pectin lyase-like superfamily protein [more]
AT1G48100.17.7e-15759.28Pectin lyase-like superfamily protein [more]
AT1G56710.11.8e-15060.39Pectin lyase-like superfamily protein [more]
AT5G14650.12.6e-9643.66Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 198..247
e-value: 1100.0
score: 7.0
coord: 248..269
e-value: 540.0
score: 9.1
coord: 330..351
e-value: 1500.0
score: 6.0
coord: 301..322
e-value: 1.1
score: 18.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 159..407
e-value: 8.3E-80
score: 268.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 278..291
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 63..445
e-value: 5.5E-113
score: 380.0
NoneNo IPR availablePANTHERPTHR31375:SF119POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEINcoord: 20..444
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 20..444
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 66..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028787.1PI0028787.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity