Homology
BLAST of PI0028598 vs. ExPASy Swiss-Prot
Match:
O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)
HSP 1 Score: 655.6 bits (1690), Expect = 6.8e-187
Identity = 355/744 (47.72%), Postives = 490/744 (65.86%), Query Frame = 0
Query: 21 LTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPS--KLIHTYNHAIS 80
L + YIVH++ + MP F HH W+S+T+ S+ S SS F S KL+++Y++ +
Sbjct: 21 LAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSIKASVPSSVDRFHSAPKLVYSYDNVLH 80
Query: 81 GFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNSNSGLLPISKYGSDVII 140
GF A L+ +L ALK PG++++ D +V TTH+S FL LN +SGL P S G DVI+
Sbjct: 81 GFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPASGLGQDVIV 140
Query: 141 GFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMARSP 200
+D+GIWP+S SF D+ M EIP RWKG C+ T FN S CN+KLIGA +FNKG++A P
Sbjct: 141 AVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYFNKGILANDP 200
Query: 201 NATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEEG 260
I+MNS RDT GHGTH ++ AG++ K S FGY GTARGVAPRAR+A+YK + EG
Sbjct: 201 TVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLAVYKFSFNEG 260
Query: 261 NSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATFAAVERGIFVATSAGNNGP 320
SD+IAA+DQA++DGVD+IS+S G +PLY+D ++IA+F A+ +G+ V+ SAGN GP
Sbjct: 261 TFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLVSASAGNRGP 320
Query: 321 QLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLFPLNTTMSLSPLPIVF--- 380
+ +L+NG+PW+L V +G DR F GT+TL NG+ + G SLFP + S P+++
Sbjct: 321 GIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAFVRDS--PVIYNKT 380
Query: 381 MGGCQN---LKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIFISNISDWDNLIQ 440
+ C + L +++ IV+C+DN +S Q+ + A++ IFIS
Sbjct: 381 LSDCSSEELLSQVENPENTIVICDDNGDFS--DQMRIITRARLKAAIFISEDPGVFRSAT 440
Query: 441 TPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARYSSRGPSQSCPFV 500
P P + +N G + +Y+ S P A + F +T L TKPAP+VA S+RGPS+S +
Sbjct: 441 FPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSARGPSRSYLGI 500
Query: 501 LKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVI-SGTSMSCPHAAGVAALLKGAHP 560
KPDI+APG ILA++P NV A + + + S ++ SGTSM+ PHAAG+AA+LK AHP
Sbjct: 501 SKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGIAAMLKAAHP 560
Query: 561 LWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKAIDPDLIYDVR 620
WSP+AIRSAMMTTAD +DNT+ IKD N NK ATPL MG+GHV+PN+A+DP L+YD
Sbjct: 561 EWSPSAIRSAMMTTADPLDNTRKPIKDSDN-NKAATPLDMGAGHVDPNRALDPGLVYDAT 620
Query: 621 IQDYVNVLCALNYTENQIRIITRSD-SNDCENPSLDLNYPSFIIIVNSSDPKTGKRKILG 680
QDYVN+LC+LN+TE Q + I RS S++C NPS DLNYPSFI + + T +
Sbjct: 621 PQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGNFT---LLEQ 680
Query: 681 EFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFELKI--AGSVTESD 740
+FKRT+T +G+ ATY+AKL+ K + V P+IL FK KN+K S+ L I G +S
Sbjct: 681 KFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIRYIGDEGQSR 740
Query: 741 VVFGYLSWVEVGGGHIVQSPIVVA 753
V G ++WVE G H V+SPIV +
Sbjct: 741 NV-GSITWVEQNGNHSVRSPIVTS 755
BLAST of PI0028598 vs. ExPASy Swiss-Prot
Match:
Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)
HSP 1 Score: 591.7 bits (1524), Expect = 1.2e-167
Identity = 338/765 (44.18%), Postives = 476/765 (62.22%), Query Frame = 0
Query: 11 LIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLI 70
L+ F++ + ET YI+HM+ +A P PF+ H SW+S T++SV+ ++ K+I
Sbjct: 9 LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI-------TNRKPKII 68
Query: 71 HTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNSNSGLLPIS 130
+ Y ++ GF A LT S+L+ LK+ PGY++ D V + TT S F+GLNS SG P+S
Sbjct: 69 YAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVS 128
Query: 131 KYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFN 190
YG+ ++IG +DTGIWPDS SF+D+ + +PS+WKG CE ++ S CNKKLIGA+ FN
Sbjct: 129 NYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNSS---SLCNKKLIGAKVFN 188
Query: 191 KGLMARSPNATIS----MNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRA 250
KGL A +P+ + +S DTIGHGTH + AAG++VK AS+F Y +GTA G+AP A
Sbjct: 189 KGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHA 248
Query: 251 RVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGL--------DGVPLYKDPVAIA 310
+AIYKA WEEG SDVIAAIDQAI DGV VISLS+GL DG L DP+A+A
Sbjct: 249 HLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVA 308
Query: 311 TFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSS 370
+FAA+++G+FV TS GN+GP +L NGAPW++ V AGT+ R F GT+T N VS S
Sbjct: 309 SFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPS 368
Query: 371 LFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALG 430
LFP P+ + G +N K + +IVVC N+ ++ S++ +++ A
Sbjct: 369 LFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGAAAV 428
Query: 431 IFISN-ISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKS-SDPKAEVNFHKTILGTKPAPI 490
+ I++ + + + I+ FP F+ H I+ Y + ++ A++ F KT++GTKPAP
Sbjct: 429 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 488
Query: 491 VARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSC 550
V YSSRGP S P +LKPDI+APG IL++WP + P++S FN+++GTSM+
Sbjct: 489 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 548
Query: 551 PHAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHV 610
PH AGVAAL+K HP WSP+AI+SA+MTTA +DN PLA+G+GHV
Sbjct: 549 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 608
Query: 611 NPNKAIDPDLIYDVRIQDYVNVLC-ALNYTENQIRIITRSDSND-CENPSLDLNYPSFII 670
+ NK ++P LIYD QD++N LC + I IITRS+ +D C+ PS LNYPS I
Sbjct: 609 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 668
Query: 671 IVNS--SDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQ 730
S S PK FKRTLT +GE + +Y ++RG+KG V V+PK L F KN+
Sbjct: 669 YFTSDQSSPKI--------FKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 728
Query: 731 KLSFELKI-AGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTRV 757
KLS+ +++ + + +VV+G +SWV+ S VVA + V
Sbjct: 729 KLSYTVRLESPRGLQENVVYGLVSWVDEDEAEFEVSCSVVATSLV 733
BLAST of PI0028598 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 515.8 bits (1327), Expect = 8.4e-145
Identity = 302/756 (39.95%), Postives = 435/756 (57.54%), Query Frame = 0
Query: 22 TETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLIHTYNHAISGFC 81
T YI+ +N + P+ F +HH WY++ ++S S L++TY + GF
Sbjct: 25 TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------ESSLLYTYTTSFHGFS 84
Query: 82 ASLTPSQLEALKNSPGYLTSVLDSSVH-VDTTHSSHFLGLNSNSGLLPISKYGSDVIIGF 141
A L ++ ++L +S + + + ++ + TT + FLGLNS G+ + + VIIG
Sbjct: 85 AYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGV 144
Query: 142 VDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMARSPNA 201
+DTG+WP+S SF+D M EIPS+WKGECE+ + F+ CNKKLIGAR F+KG S
Sbjct: 145 LDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGG 204
Query: 202 TISMN---STRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEE 261
S S RD GHGTHTSTTAAGS V+ ASF GY GTARG+A RARVA YK W
Sbjct: 205 FSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWST 264
Query: 262 GNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATFAAVERGIFVATSAGNNG 321
G SD++AA+D+AI DGVDV+SLS+G P Y+D +AI F+A+ERG+FV+ SAGN+G
Sbjct: 265 GCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSG 324
Query: 322 PQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLFPLNTTMSLSPLPIVFMG 381
P ++ N APWV+ V AGT+DRDF L NG + G SL+ M PL +V+
Sbjct: 325 PTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKPLELVYNK 384
Query: 382 GCQNLKKLKRIGY--------KIVVCEDNDGYSLTSQVDNVQTAQVALGIFISN-ISDWD 441
G + L G KIVVC+ G + + V LG+ ++N + +
Sbjct: 385 GNSSSSNLCLPGSLDSSIVRGKIVVCD--RGVNARVEKGAVVRDAGGLGMIMANTAASGE 444
Query: 442 NLIQTP--FPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARYSSRGPS 501
L+ P+I + G ++++Y+ S P A + F T+L KP+P+VA +SSRGP+
Sbjct: 445 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPN 504
Query: 502 QSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAAGVAALL 561
P +LKPD++ PG ILA W + ++ ++FN++SGTSMSCPH +G+A LL
Sbjct: 505 TVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLL 564
Query: 562 KGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKAIDPDL 621
K AHP WSP+AI+SA+MTTA V+DNT + D + N + P A GSGHV+P KA+ P L
Sbjct: 565 KAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVDPQKALSPGL 624
Query: 622 IYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLD---LNYPSFIIIVNSSDPKT 681
+YD+ ++Y+ LC+L+YT + I I + S +C D LNYPSF ++
Sbjct: 625 VYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFG------ 684
Query: 682 GKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFK----RKNQKLSFELK 741
GKR + + R +T +G + Y+ + G + VKP L FK +K ++F K
Sbjct: 685 GKRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSK 744
Query: 742 IAGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTR 756
S+T + FG ++W H V+SP+ + R
Sbjct: 745 KGVSMT-NKAEFGSITW--SNPQHEVRSPVAFSWNR 753
BLAST of PI0028598 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 510.4 bits (1313), Expect = 3.5e-143
Identity = 310/760 (40.79%), Postives = 438/760 (57.63%), Query Frame = 0
Query: 27 YIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
YIVH++ A P F +H WY+ SS +S +S P +IHTY+ GF A LT
Sbjct: 28 YIVHVDHEAKPSIFPTHFHWYT--------SSLASLTSSPPSIIHTYDTVFHGFSARLTS 87
Query: 87 SQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNS--NSGLLPISKYGSDVIIGFVDTG 146
L + P ++ + + H+ TT S FLGL S +GLL S +GSD++IG +DTG
Sbjct: 88 QDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTG 147
Query: 147 IWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMARS--PNATI 206
+WP+ SF+D + +P +WKG+C S F S CN+KL+GARFF G A + N T
Sbjct: 148 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 207
Query: 207 SMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVS 266
S RD+ GHGTHT++ +AG YV AS GY G A G+AP+AR+A YK W G S
Sbjct: 208 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDS 267
Query: 267 DVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATFAAVERGIFVATSAGNNGPQLET 326
D++AA D A++DGVDVISLS+G VP Y D +AI F A++RGIFV+ SAGN GP T
Sbjct: 268 DILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALT 327
Query: 327 LHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLFPLNTTMSLSPLPIVF----MGG 386
+ N APW+ V AGT+DRDF V L NG + G S++ P+V+ +GG
Sbjct: 328 VTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGG 387
Query: 387 CQNLKKL--------KRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIFISN-ISDWDN 446
L + KIV+C+ G + + + LG+ I+N + D +
Sbjct: 388 DGYSSSLCLEGSLDPNLVKGKIVLCD--RGINSRATKGEIVRKNGGLGMIIANGVFDGEG 447
Query: 447 LIQT--PFPSIFLNPYHGSIIKDYIHKSS------DPKAEVNFHKTILGTKPAPIVARYS 506
L+ P+ + G I+ YI +SS P A + F T LG +PAP+VA +S
Sbjct: 448 LVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFS 507
Query: 507 SRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAAG 566
+RGP+ P +LKPD++APG ILA+WP + V S ++FN++SGTSM+CPH +G
Sbjct: 508 ARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSG 567
Query: 567 VAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKA 626
+AALLK AHP WSPAAIRSA++TTA VDN+ + D N ++ + GSGHV+P KA
Sbjct: 568 LAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSGHVHPTKA 627
Query: 627 IDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSL-----DLNYPSFIIIV 686
+DP L+YD+ DY+N LC NYT I ITR + DC+ +LNYPSF ++
Sbjct: 628 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNYPSFSVVF 687
Query: 687 NSSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSF 746
+ G+ K+ F RT+T +G++ + YE K+R +G V V+P+ L F+R QKLSF
Sbjct: 688 Q----QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSF 747
Query: 747 ELKIAGSVTE-----SDVVFGYLSWVEVGGGHIVQSPIVV 752
+++ + + ++V G++ W + G V SP+VV
Sbjct: 748 VVRVKTTEVKLSPGATNVETGHIVWSD--GKRNVTSPLVV 769
BLAST of PI0028598 vs. ExPASy Swiss-Prot
Match:
O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)
HSP 1 Score: 500.7 bits (1288), Expect = 2.8e-140
Identity = 299/754 (39.66%), Postives = 427/754 (56.63%), Query Frame = 0
Query: 27 YIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
+I ++ +MP F +H+ WYS + S+++H Y+ GF A +TP
Sbjct: 28 FIFRIDGGSMPSIFPTHYHWYSTEFAE------------ESRIVHVYHTVFHGFSAVVTP 87
Query: 87 SQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNSNSGLLPISKYGSDVIIGFVDTGIW 146
+ + L+N P L D + TT S FLGL + GL S YGSDVIIG DTGIW
Sbjct: 88 DEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGVFDTGIW 147
Query: 147 PDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMAR---SPNATIS 206
P+ SF+D + IP RW+G CE+ F+ CN+K+IGARFF KG A N T+
Sbjct: 148 PERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVE 207
Query: 207 MNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSV-S 266
S RD GHGTHTS+TAAG + +AS GY G A+GVAP+AR+A YK W++ + S
Sbjct: 208 FLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDS 267
Query: 267 DVIAAIDQAISDGVDVISLSI-GLDGV--PLYKDPVAIATFAAVERGIFVATSAGNNGPQ 326
D++AA D A+ DGVDVIS+SI G DG+ P Y DP+AI ++ A +GIFV++SAGN GP
Sbjct: 268 DILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPN 327
Query: 327 LETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLF---PLNTTMSLSPLPIVFM 386
++ N APWV V A T+DR+F L +G + G SL+ PLN M P+V+
Sbjct: 328 GMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM----FPVVYP 387
Query: 387 GG---------CQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIFISNISDW 446
G +N K++ KIV+C+ + + + V + I + S+
Sbjct: 388 GKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGM-ILANGASNG 447
Query: 447 DNLIQTP--FPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARYSSRGP 506
+ L+ P+ + G IK Y +P A ++F TI+G KPAP++A +S RGP
Sbjct: 448 EGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGP 507
Query: 507 SQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAAGVAAL 566
+ P +LKPD++APG ILA+W V + S P ++FN++SGTSM+CPH +G AAL
Sbjct: 508 NGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAAL 567
Query: 567 LKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKAIDPD 626
LK AHP WSPA IRSAMMTT ++VDN+ S+ D + K ATP GSGH+N +A++P
Sbjct: 568 LKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGHLNLGRAMNPG 627
Query: 627 LIYDVRIQDYVNVLCALNYTENQIRIITRSDSN--DCENPSL-DLNYPSFIIIVNSSDPK 686
L+YD+ DY+ LC++ Y I++ITR+ PS +LNYPS + P
Sbjct: 628 LVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVF----PT 687
Query: 687 TGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFELKIAG 746
+ + RT T +G+ A Y A++ +G V VKP L F ++ S+ + +
Sbjct: 688 NRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTV 747
Query: 747 S-----VTESDVVFGYLSWVEVGGGHIVQSPIVV 752
+ + E+ VFG ++W + GG H+V+SPIVV
Sbjct: 748 NTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVV 758
BLAST of PI0028598 vs. ExPASy TrEMBL
Match:
A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)
HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 704/763 (92.27%), Postives = 728/763 (95.41%), Query Frame = 0
Query: 1 MAARLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLH---- 60
MA RLYFWFSL PIFWL PILTETHNYIVHMNSAAMPKPFAS HSWYSAT+SSVLH
Sbjct: 1 MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
Query: 61 ---SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTH 120
SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYL+SVLDSSVHVDTTH
Sbjct: 61 SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120
Query: 121 SSHFLGLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTH 180
SSHFLGL+SN GLLPISKYGSDVIIGFVDTGIWPDSESFND+ MSEIPSRWKGECE+STH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180
Query: 181 FNVSFCNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFG 240
FNVSFCN KLIGARFFNKGL+A+ PNATISMNSTRDTIGHGTHTST AAGSYVKEASFFG
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240
Query: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKD 300
YGRGTARGVAPRARVAIYKAIW+EGNSVSDV+AAIDQAISDGVDVISLSIG+DG+PLY D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300
Query: 301 PVAIATFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVS 360
PV+IATFAAVERGIFVATSAGNNGPQL T+HNGAPWVLNV AGTMDRDFGGT+TLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360
Query: 361 VLGSSLFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420
VLGSSLFPLNTTM LSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420
Query: 421 QVALGIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKP 480
+V LGIFISNI DWDNLIQTPFPSIF+NPYHG+IIKDYIHKSSDPKAEVNFHKTILGTKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480
Query: 481 APIVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTS 540
AP VARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQN+ AMDVNSTPIY+KFNVISGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540
Query: 541 MSCPHAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGS 600
MSCPHAAGVAALLKGAHP WSPAAIRSAMMTTADVVDNTQTSIKDIGN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600
Query: 601 GHVNPNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFI 660
GHVNPNKA+DPDLIYDV IQDYVNVLCALNYTENQIRIITRSDSN+CENPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660
Query: 661 IIVNSSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQK 720
II NSSD KTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGF VRVKP+IL+FKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720
Query: 721 LSFELKIAGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTRV 757
LSFELKIAGS ES+VVFGYLSW EVGG HIVQSPIVVAG RV
Sbjct: 721 LSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRV 763
BLAST of PI0028598 vs. ExPASy TrEMBL
Match:
A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)
HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 704/763 (92.27%), Postives = 728/763 (95.41%), Query Frame = 0
Query: 1 MAARLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLH---- 60
MA RLYFWFSL PIFWL PILTETHNYIVHMNSAAMPKPFAS HSWYSAT+SSVLH
Sbjct: 1 MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
Query: 61 ---SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTH 120
SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYL+SVLDSSVHVDTTH
Sbjct: 61 SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120
Query: 121 SSHFLGLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTH 180
SSHFLGL+SN GLLPISKYGSDVIIGFVDTGIWPDSESFND+ MSEIPSRWKGECE+STH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180
Query: 181 FNVSFCNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFG 240
FNVSFCN KLIGARFFNKGL+A+ PNATISMNSTRDTIGHGTHTST AAGSYVKEASFFG
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240
Query: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKD 300
YGRGTARGVAPRARVAIYKAIW+EGNSVSDV+AAIDQAISDGVDVISLSIG+DG+PLY D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300
Query: 301 PVAIATFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVS 360
PV+IATFAAVERGIFVATSAGNNGPQL T+HNGAPWVLNV AGTMDRDFGGT+TLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360
Query: 361 VLGSSLFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420
VLGSSLFPLNTTM LSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420
Query: 421 QVALGIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKP 480
+V LGIFISNI DWDNLIQTPFPSIF+NPYHG+IIKDYIHKSSDPKAEVNFHKTILGTKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480
Query: 481 APIVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTS 540
AP VARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQN+ AMDVNSTPIY+KFNVISGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540
Query: 541 MSCPHAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGS 600
MSCPHAAGVAALLKGAHP WSPAAIRSAMMTTADVVDNTQTSIKDIGN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600
Query: 601 GHVNPNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFI 660
GHVNPNKA+DPDLIYDV IQDYVNVLCALNYTENQIRIITRSDSN+CENPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660
Query: 661 IIVNSSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQK 720
II NSSD KTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGF VRVKP+IL+FKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720
Query: 721 LSFELKIAGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTRV 757
LSFELKIAGS ES+VVFGYLSW EVGG HIVQSPIVVAG RV
Sbjct: 721 LSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRV 763
BLAST of PI0028598 vs. ExPASy TrEMBL
Match:
A0A0A0LKL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1)
HSP 1 Score: 1373.6 bits (3554), Expect = 0.0e+00
Identity = 687/756 (90.87%), Postives = 717/756 (94.84%), Query Frame = 0
Query: 1 MAARLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSS 60
MAARLYFWFSLIPIFWL PILTET NYIVHMNSAAMPKPFAS HSWYSAT+SS+LHSSSS
Sbjct: 1 MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS 60
Query: 61 SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGL 120
SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYL+SVLDSSVHVDTTHSSHFLGL
Sbjct: 61 SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL 120
Query: 121 NSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCN 180
+SN GLLPISKYGSDVIIGFVDTGIWPDSESF D+ MSEIPS+WKGECE+STHFNVSFCN
Sbjct: 121 SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN 180
Query: 181 KKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTAR 240
KLIGARFFNKGL++ P ATIS+NSTRDTIGHGTHTSTTAAGSY+KEASFFGYGRGTAR
Sbjct: 181 NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR 240
Query: 241 GVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATF 300
GVAPRARVAIYKAIWEEGNSVSDV+AAIDQAISDGVDVISLSIG+DGVPLY DPVAIATF
Sbjct: 241 GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF 300
Query: 301 AAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLF 360
AAVERGIFVATSAGNNGPQLET+HNGAPW+LNV AGTMDRDFGGT+TLSNGVSVLGSSLF
Sbjct: 301 AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF 360
Query: 361 PLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIF 420
PLN T LSPLPIVFMGGCQNLKKL+R GYKIVVCED+DGYSLTSQVDNVQTA VALGIF
Sbjct: 361 PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF 420
Query: 421 ISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARY 480
ISNI DWDNLIQTPFPSIFLNPYHG+IIKDYIHKSSDPKAEV FHKTIL TKPAP+VARY
Sbjct: 421 ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY 480
Query: 481 SSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAA 540
SSRGPSQSCPFVLKPDIMAPGD ILASWPQNV AMDVNSTPIYSKFNVISGTSMSCPHAA
Sbjct: 481 SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA 540
Query: 541 GVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNK 600
GVAALLKGAHP WSPAAIRSAMMTTAD++DNTQT IKD GN NKFATPLAMGSGHVNPNK
Sbjct: 541 GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK 600
Query: 601 AIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFIIIVNSSD 660
AIDPDLIYDV IQDYVNVLCALNYTENQIRIITRSDSN+CENPSLDLNYPSFI+IVNSSD
Sbjct: 601 AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD 660
Query: 661 PKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFELKI 720
KT KRKI GEFKRTLTKIGE+RATYEAKL GMKGF VRVKP L FKRKNQKLSFELKI
Sbjct: 661 SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI 720
Query: 721 AGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTRV 757
AGS ES++VFGYLSW EVGGGHI+QSPIVV+G R+
Sbjct: 721 AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRL 756
BLAST of PI0028598 vs. ExPASy TrEMBL
Match:
A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)
HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 606/763 (79.42%), Postives = 673/763 (88.20%), Query Frame = 0
Query: 4 RLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSV------LHS 63
R++F SL+ I R LTET NYIVHM+ AAMPKPFA+HHSWYSAT+SSV L +
Sbjct: 6 RMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65
Query: 64 SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHF 123
++SSSSS PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY++SV DSSV DTTHSS+F
Sbjct: 66 TTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNF 125
Query: 124 LGLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVS 183
L L NSGLLPIS YGSDVIIGFVDTG+WP+SESFND A+S+IPSRWKGECE+ THFN S
Sbjct: 126 LALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNAS 185
Query: 184 FCNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRG 243
CNKKLIG RFFNKGL+A+ PN TISMNSTRDT GHGTHTSTTAAGSYVKEASFFGYG+G
Sbjct: 186 LCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQG 245
Query: 244 TARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAI 303
TARGVAPRARVAIYKAIW+EGN VSDVIAAIDQAISDGVDVISLS+GLDGVPLY+DPVAI
Sbjct: 246 TARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAI 305
Query: 304 ATFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGS 363
ATFAA+ERGIFVATSAGN GPQ T+H+GAPWVLNV AGTMDRDFGGT+TL+NGVSVLGS
Sbjct: 306 ATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGS 365
Query: 364 SLFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVAL 423
SLF LN+ + LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL QVDNVQ+A++A+
Sbjct: 366 SLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAV 425
Query: 424 GIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIV 483
G+FISNISDWDNLIQT FPSIFLN YHG++IKDYI +SS+PKA VNFHKTI+GTKPAP V
Sbjct: 426 GVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSV 485
Query: 484 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCP 543
ARYSSRGPS+SCP VLKPDIMAPGDAILASWPQ VAA DV S PIYSKFNV+SGTSM+CP
Sbjct: 486 ARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACP 545
Query: 544 HAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVN 603
HAAGVAALLKGAHP WSPAAIRSAMMTTADVVDNTQT IKD+GNKNK ATPLAMGSGHVN
Sbjct: 546 HAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVN 605
Query: 604 PNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFIIIVN 663
PNKAIDP LIYD+ I+DY N+LCALNYT+NQI+ ITRS SNDCE PSLDLNYPSFII VN
Sbjct: 606 PNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVN 665
Query: 664 SSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFE 723
+SD ++G+R+I EFKR +T IGE ATY AK+ MKG VV V+PK+LKFKRKNQ LSF+
Sbjct: 666 ASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFK 725
Query: 724 LKIAGSVT---ESDVVFGYLSWVEVGGGHIVQSPIVVAGTRVH 758
LKI G V+ ESDVVFGYL+WVEVGGGH VQSPIVVAG R H
Sbjct: 726 LKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLRSH 768
BLAST of PI0028598 vs. ExPASy TrEMBL
Match:
A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)
HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 601/767 (78.36%), Postives = 675/767 (88.01%), Query Frame = 0
Query: 4 RLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVL-------H 63
R++FW SL+ IF R LT+T NYIVHM+ AAMPKPFA+HHSWYSAT+SSVL
Sbjct: 6 RMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65
Query: 64 SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSH 123
++SSSSSS PSKLIH Y HAI+GF ASLTPSQL+ALKNSPGY++S+ DSSV DTTHSS+
Sbjct: 66 TTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSN 125
Query: 124 FLGLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNV 183
FL L+ NSGLLPIS YGSDVIIGFVDTG+WP+SESFND+ +S+IPSRWKGECE+ THFN
Sbjct: 126 FLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNA 185
Query: 184 SFCNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGR 243
S CNKKLIG RFFNKGL+++ PN TISMNSTRDT GHGTHTSTTAAGSYVKEASFFGYG+
Sbjct: 186 SLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQ 245
Query: 244 GTARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVA 303
GTARGVAPRARVAIYKAIW+EGN+VSDVIAAIDQAISDGVDVISLS+GLDGVPLY+DPVA
Sbjct: 246 GTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVA 305
Query: 304 IATFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLG 363
IATFAA+ERGIFVATSAGN GPQ T+H+GAPWVLNV AGTMDRDFGGT+TL+NGVSVLG
Sbjct: 306 IATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLG 365
Query: 364 SSLFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVA 423
SSLFPLN+ ++LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL QVDNV++A++A
Sbjct: 366 SSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIA 425
Query: 424 LGIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPI 483
+G+FISNISDWDNLIQT FPSIFLN YHG++IKDYI +SS+PKA VNFHKTI+GTKPAP
Sbjct: 426 VGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPS 485
Query: 484 VARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSC 543
VARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQNVAA DV S PIYSKFNV+SGTSM+C
Sbjct: 486 VARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMAC 545
Query: 544 PHAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHV 603
PHAAGVAALLKGAHP WSPAAIRSAMMTTAD+VDNTQT IKD+GNKNK ATPLAMGSGHV
Sbjct: 546 PHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHV 605
Query: 604 NPNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFIIIV 663
NPNKAIDP LIYD+ I+DY N+LCALNYT+NQI+ ITRS SN CE LDLNYPSFI+ V
Sbjct: 606 NPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTV 665
Query: 664 NSSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSF 723
N+SD +TG+ ++ EFKR +T IGE ATY AK+ MKG VV V+P LKFKRKNQ LSF
Sbjct: 666 NASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSF 725
Query: 724 ELKIAGSVT---ESDVVFGYLSWVEVGGGHIVQSPI---VVAGTRVH 758
+LKI G V+ ESDVVFGYL+WVEVGGGH VQSPI VVAG R H
Sbjct: 726 KLKIRGHVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVAVVAGLRSH 772
BLAST of PI0028598 vs. NCBI nr
Match:
XP_008444560.1 (PREDICTED: subtilisin-like protease SBT1.9 [Cucumis melo] >KAA0060951.1 subtilisin-like protease SBT1.9 [Cucumis melo var. makuwa])
HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 704/763 (92.27%), Postives = 728/763 (95.41%), Query Frame = 0
Query: 1 MAARLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLH---- 60
MA RLYFWFSL PIFWL PILTETHNYIVHMNSAAMPKPFAS HSWYSAT+SSVLH
Sbjct: 1 MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
Query: 61 ---SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTH 120
SSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYL+SVLDSSVHVDTTH
Sbjct: 61 SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120
Query: 121 SSHFLGLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTH 180
SSHFLGL+SN GLLPISKYGSDVIIGFVDTGIWPDSESFND+ MSEIPSRWKGECE+STH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180
Query: 181 FNVSFCNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFG 240
FNVSFCN KLIGARFFNKGL+A+ PNATISMNSTRDTIGHGTHTST AAGSYVKEASFFG
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240
Query: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKD 300
YGRGTARGVAPRARVAIYKAIW+EGNSVSDV+AAIDQAISDGVDVISLSIG+DG+PLY D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300
Query: 301 PVAIATFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVS 360
PV+IATFAAVERGIFVATSAGNNGPQL T+HNGAPWVLNV AGTMDRDFGGT+TLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360
Query: 361 VLGSSLFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420
VLGSSLFPLNTTM LSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420
Query: 421 QVALGIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKP 480
+V LGIFISNI DWDNLIQTPFPSIF+NPYHG+IIKDYIHKSSDPKAEVNFHKTILGTKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480
Query: 481 APIVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTS 540
AP VARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQN+ AMDVNSTPIY+KFNVISGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540
Query: 541 MSCPHAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGS 600
MSCPHAAGVAALLKGAHP WSPAAIRSAMMTTADVVDNTQTSIKDIGN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600
Query: 601 GHVNPNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFI 660
GHVNPNKA+DPDLIYDV IQDYVNVLCALNYTENQIRIITRSDSN+CENPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660
Query: 661 IIVNSSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQK 720
II NSSD KTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGF VRVKP+IL+FKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720
Query: 721 LSFELKIAGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTRV 757
LSFELKIAGS ES+VVFGYLSW EVGG HIVQSPIVVAG RV
Sbjct: 721 LSFELKIAGSAEESNVVFGYLSWAEVGGRHIVQSPIVVAGMRV 763
BLAST of PI0028598 vs. NCBI nr
Match:
XP_004143027.3 (subtilisin-like protease SBT3 [Cucumis sativus] >KGN62450.1 hypothetical protein Csa_018640 [Cucumis sativus])
HSP 1 Score: 1373.6 bits (3554), Expect = 0.0e+00
Identity = 687/756 (90.87%), Postives = 717/756 (94.84%), Query Frame = 0
Query: 1 MAARLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSS 60
MAARLYFWFSLIPIFWL PILTET NYIVHMNSAAMPKPFAS HSWYSAT+SS+LHSSSS
Sbjct: 1 MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHSSSS 60
Query: 61 SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGL 120
SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYL+SVLDSSVHVDTTHSSHFLGL
Sbjct: 61 SSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGL 120
Query: 121 NSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCN 180
+SN GLLPISKYGSDVIIGFVDTGIWPDSESF D+ MSEIPS+WKGECE+STHFNVSFCN
Sbjct: 121 SSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTHFNVSFCN 180
Query: 181 KKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTAR 240
KLIGARFFNKGL++ P ATIS+NSTRDTIGHGTHTSTTAAGSY+KEASFFGYGRGTAR
Sbjct: 181 NKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFGYGRGTAR 240
Query: 241 GVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATF 300
GVAPRARVAIYKAIWEEGNSVSDV+AAIDQAISDGVDVISLSIG+DGVPLY DPVAIATF
Sbjct: 241 GVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDDPVAIATF 300
Query: 301 AAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLF 360
AAVERGIFVATSAGNNGPQLET+HNGAPW+LNV AGTMDRDFGGT+TLSNGVSVLGSSLF
Sbjct: 301 AAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVSVLGSSLF 360
Query: 361 PLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIF 420
PLN T LSPLPIVFMGGCQNLKKL+R GYKIVVCED+DGYSLTSQVDNVQTA VALGIF
Sbjct: 361 PLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTANVALGIF 420
Query: 421 ISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARY 480
ISNI DWDNLIQTPFPSIFLNPYHG+IIKDYIHKSSDPKAEV FHKTIL TKPAP+VARY
Sbjct: 421 ISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKPAPMVARY 480
Query: 481 SSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAA 540
SSRGPSQSCPFVLKPDIMAPGD ILASWPQNV AMDVNSTPIYSKFNVISGTSMSCPHAA
Sbjct: 481 SSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTSMSCPHAA 540
Query: 541 GVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNK 600
GVAALLKGAHP WSPAAIRSAMMTTAD++DNTQT IKD GN NKFATPLAMGSGHVNPNK
Sbjct: 541 GVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGSGHVNPNK 600
Query: 601 AIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFIIIVNSSD 660
AIDPDLIYDV IQDYVNVLCALNYTENQIRIITRSDSN+CENPSLDLNYPSFI+IVNSSD
Sbjct: 601 AIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIMIVNSSD 660
Query: 661 PKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFELKI 720
KT KRKI GEFKRTLTKIGE+RATYEAKL GMKGF VRVKP L FKRKNQKLSFELKI
Sbjct: 661 SKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQKLSFELKI 720
Query: 721 AGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTRV 757
AGS ES++VFGYLSW EVGGGHI+QSPIVV+G R+
Sbjct: 721 AGSARESNIVFGYLSWAEVGGGHIIQSPIVVSGMRL 756
BLAST of PI0028598 vs. NCBI nr
Match:
XP_038885776.1 (subtilisin-like protease SBT3 [Benincasa hispida])
HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 647/761 (85.02%), Postives = 698/761 (91.72%), Query Frame = 0
Query: 1 MAARLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSS 60
MAA LYFWFSLIPI WL PILTET NYIVHMNSAAMPKPFAS HSWYSAT+SSVLHSSSS
Sbjct: 1 MAAGLYFWFSLIPILWLCPILTETDNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60
Query: 61 SSS--SFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHFL 120
SSS S SKL H+YNHAI GF ASL+PS+LEALKNSPGY++SVLDSSV VDTTHSSHFL
Sbjct: 61 SSSSASSSSKLTHSYNHAIDGFSASLSPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFL 120
Query: 121 GLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSF 180
GL+SN GLLPIS YGSDVIIGFVDTGIWP+ ESFND+ MSEIPSRWKGECE THFN S
Sbjct: 121 GLSSNFGLLPISNYGSDVIIGFVDTGIWPEGESFNDDGMSEIPSRWKGECERGTHFNASL 180
Query: 181 CNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGT 240
CNKKLIGARFFNKGL+A+ PN TISMNSTRDTIGHGTHT+T AAGSYVKEASFFGYGRGT
Sbjct: 181 CNKKLIGARFFNKGLLAKFPNITISMNSTRDTIGHGTHTATIAAGSYVKEASFFGYGRGT 240
Query: 241 ARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIA 300
ARGVAPRAR+AIYKAIWEEGN+VSDVIAAIDQAISDGVDVISLS+GLDGVPLY+DPVAIA
Sbjct: 241 ARGVAPRARIAIYKAIWEEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYQDPVAIA 300
Query: 301 TFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSS 360
TFAAVERGIFVAT+ GNNGPQL T+HNGAPWVLNV AGTMDRDFGGT+TLSNGV++LGSS
Sbjct: 301 TFAAVERGIFVATTTGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVTILGSS 360
Query: 361 LFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDG-YSLTSQVDNVQTAQVAL 420
LFPLN+TM +SPLPIVFMGGCQNLKKLKRIGYKI VC+D D YSL+SQVDNVQ+A VAL
Sbjct: 361 LFPLNSTMDVSPLPIVFMGGCQNLKKLKRIGYKIAVCDDKDEYYSLSSQVDNVQSANVAL 420
Query: 421 GIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIV 480
G+FISNISDWDNLIQT FPSIFL+PY+G+IIKDYI KSSDPKAEVNFHKTILGTKPAP V
Sbjct: 421 GVFISNISDWDNLIQTSFPSIFLSPYNGNIIKDYIKKSSDPKAEVNFHKTILGTKPAPSV 480
Query: 481 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCP 540
ARYSSRGPS SCPFVLKPDIMAPGDAILASWPQNVAAM V S PIY+KFN++SGTSMSCP
Sbjct: 481 ARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMYVYSDPIYTKFNIMSGTSMSCP 540
Query: 541 HAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVN 600
HAAGVAALLK H WSPAAIRSAMMTTADVVDN QTSIKDIGNKN+FA+PLAMGSGH+N
Sbjct: 541 HAAGVAALLKVVHHGWSPAAIRSAMMTTADVVDNNQTSIKDIGNKNRFASPLAMGSGHIN 600
Query: 601 PNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFIIIVN 660
PNKAIDP L+YD+ IQDYVN+LCALNYT++QI+ ITR DSNDCENPS+DLNYPSFIIIVN
Sbjct: 601 PNKAIDPGLVYDMGIQDYVNLLCALNYTKSQIQTITRLDSNDCENPSMDLNYPSFIIIVN 660
Query: 661 SSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFE 720
+S+ KT K +ILGEFKRTLTKIGE RATYEAKLRGMKGF V V PK L+FKRKNQKLSF+
Sbjct: 661 TSNSKTSKGEILGEFKRTLTKIGEKRATYEAKLRGMKGFKVGVNPKKLEFKRKNQKLSFK 720
Query: 721 LKIAGSV---TESDVVFGYLSWVEVGGGHIVQSPIVVAGTR 756
LKIAG+ E+D+VFGYLSWVEVGGGHIVQSPIVVAG R
Sbjct: 721 LKIAGTARVKRENDMVFGYLSWVEVGGGHIVQSPIVVAGLR 761
BLAST of PI0028598 vs. NCBI nr
Match:
XP_022996869.1 (subtilisin-like protease SBT1.9 [Cucurbita maxima])
HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 606/763 (79.42%), Postives = 673/763 (88.20%), Query Frame = 0
Query: 4 RLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSV------LHS 63
R++F SL+ I R LTET NYIVHM+ AAMPKPFA+HHSWYSAT+SSV L +
Sbjct: 6 RMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65
Query: 64 SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHF 123
++SSSSS PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY++SV DSSV DTTHSS+F
Sbjct: 66 TTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNF 125
Query: 124 LGLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVS 183
L L NSGLLPIS YGSDVIIGFVDTG+WP+SESFND A+S+IPSRWKGECE+ THFN S
Sbjct: 126 LALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNAS 185
Query: 184 FCNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRG 243
CNKKLIG RFFNKGL+A+ PN TISMNSTRDT GHGTHTSTTAAGSYVKEASFFGYG+G
Sbjct: 186 LCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQG 245
Query: 244 TARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAI 303
TARGVAPRARVAIYKAIW+EGN VSDVIAAIDQAISDGVDVISLS+GLDGVPLY+DPVAI
Sbjct: 246 TARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAI 305
Query: 304 ATFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGS 363
ATFAA+ERGIFVATSAGN GPQ T+H+GAPWVLNV AGTMDRDFGGT+TL+NGVSVLGS
Sbjct: 306 ATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGS 365
Query: 364 SLFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVAL 423
SLF LN+ + LSPLPIVFMG C NLKKLKR+G+KIVVCED+D YSL QVDNVQ+A++A+
Sbjct: 366 SLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAV 425
Query: 424 GIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIV 483
G+FISNISDWDNLIQT FPSIFLN YHG++IKDYI +SS+PKA VNFHKTI+GTKPAP V
Sbjct: 426 GVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSV 485
Query: 484 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCP 543
ARYSSRGPS+SCP VLKPDIMAPGDAILASWPQ VAA DV S PIYSKFNV+SGTSM+CP
Sbjct: 486 ARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACP 545
Query: 544 HAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVN 603
HAAGVAALLKGAHP WSPAAIRSAMMTTADVVDNTQT IKD+GNKNK ATPLAMGSGHVN
Sbjct: 546 HAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVN 605
Query: 604 PNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFIIIVN 663
PNKAIDP LIYD+ I+DY N+LCALNYT+NQI+ ITRS SNDCE PSLDLNYPSFII VN
Sbjct: 606 PNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVN 665
Query: 664 SSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFE 723
+SD ++G+R+I EFKR +T IGE ATY AK+ MKG VV V+PK+LKFKRKNQ LSF+
Sbjct: 666 ASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFK 725
Query: 724 LKIAGSVT---ESDVVFGYLSWVEVGGGHIVQSPIVVAGTRVH 758
LKI G V+ ESDVVFGYL+WVEVGGGH VQSPIVVAG R H
Sbjct: 726 LKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLRSH 768
BLAST of PI0028598 vs. NCBI nr
Match:
XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 598/757 (79.00%), Postives = 673/757 (88.90%), Query Frame = 0
Query: 4 RLYFWFSLIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSV------LHS 63
R++FW SL+ IF R +TET NYIVHM+ AAMPKPFA+HHSWYSAT+SSV L +
Sbjct: 6 RMFFWISLMVIFLARSTVTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65
Query: 64 SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHF 123
++SSSSS PSKLIH Y HAI+GF A+LTPSQL+ALKNSPGY++SV DSSV DTTHSS+F
Sbjct: 66 TTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNF 125
Query: 124 LGLNSNSGLLPISKYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVS 183
L L+ NSGLLPIS YGSDVIIGFVDTG+WP+SESFND+ +S+IPSRWKGECE+ THFN S
Sbjct: 126 LALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNAS 185
Query: 184 FCNKKLIGARFFNKGLMARSPNATISMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRG 243
CNKKLIG RFFNKGL+A+ PN TISMNSTRDT GHGTHTSTTAAGSYVKEASFFGYG+G
Sbjct: 186 LCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQG 245
Query: 244 TARGVAPRARVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAI 303
TARGVAPRARVAIYKAIW+EGN VSDVIAAIDQAISDGVDVI+LS+G+D VPLY+DPVAI
Sbjct: 246 TARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVIALSLGIDRVPLYEDPVAI 305
Query: 304 ATFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGS 363
ATFAA+ERGIFVATSAGN GPQ T+H+GAPWVLNV AGTMDRDFGGT+TL+NGVSVLGS
Sbjct: 306 ATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGS 365
Query: 364 SLFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVAL 423
SLFPLN+ ++LSPLPI FMG C NLKKLKR+G+KIVVCED+D YSL QVDNVQ+A++A+
Sbjct: 366 SLFPLNSAIALSPLPIFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAV 425
Query: 424 GIFISNISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIV 483
G+FISNISDWDNLIQT FPSIFLN YHG+++KDYI++SS+PKA VNFHKTI+GTKPAP V
Sbjct: 426 GVFISNISDWDNLIQTSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHKTIIGTKPAPSV 485
Query: 484 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCP 543
ARYSSRGPS+SCPFVLKPDIMAPGDAILASWPQNVAA DV S PIYSKFNV+SGTSM+CP
Sbjct: 486 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACP 545
Query: 544 HAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVN 603
HAAGVAALLKGAHP WSPAAIRSAMMTTAD+VDNTQT IKD+GNKNK ATPLAMGSGHVN
Sbjct: 546 HAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVN 605
Query: 604 PNKAIDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLDLNYPSFIIIVN 663
PNKAIDP LIYD+ I+DY N++CALNYT+NQI+ ITRS SNDCE PSLDLNYPSFII VN
Sbjct: 606 PNKAIDPGLIYDMGIEDYTNLICALNYTKNQIQTITRSTSNDCEKPSLDLNYPSFIITVN 665
Query: 664 SSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFE 723
+SD +TG+ +I EFKR +T IGE ATY AK+ MKG VV V+PK LKFKRKNQ LSF+
Sbjct: 666 ASDSETGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLKFKRKNQNLSFK 725
Query: 724 LKIAGSVT---ESDVVFGYLSWVEVGGGHIVQSPIVV 752
LKI G V+ ESDVVFGYL+WVEVGGGH VQSPIVV
Sbjct: 726 LKIRGHVSVKRESDVVFGYLNWVEVGGGHRVQSPIVV 762
BLAST of PI0028598 vs. TAIR 10
Match:
AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 591.7 bits (1524), Expect = 8.6e-169
Identity = 338/765 (44.18%), Postives = 476/765 (62.22%), Query Frame = 0
Query: 11 LIPIFWLRPILTETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLI 70
L+ F++ + ET YI+HM+ +A P PF+ H SW+S T++SV+ ++ K+I
Sbjct: 9 LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI-------TNRKPKII 68
Query: 71 HTYNHAISGFCASLTPSQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNSNSGLLPIS 130
+ Y ++ GF A LT S+L+ LK+ PGY++ D V + TT S F+GLNS SG P+S
Sbjct: 69 YAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPVS 128
Query: 131 KYGSDVIIGFVDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFN 190
YG+ ++IG +DTGIWPDS SF+D+ + +PS+WKG CE ++ S CNKKLIGA+ FN
Sbjct: 129 NYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNSS---SLCNKKLIGAKVFN 188
Query: 191 KGLMARSPNATIS----MNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRA 250
KGL A +P+ + +S DTIGHGTH + AAG++VK AS+F Y +GTA G+AP A
Sbjct: 189 KGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPHA 248
Query: 251 RVAIYKAIWEEGNSVSDVIAAIDQAISDGVDVISLSIGL--------DGVPLYKDPVAIA 310
+AIYKA WEEG SDVIAAIDQAI DGV VISLS+GL DG L DP+A+A
Sbjct: 249 HLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAVA 308
Query: 311 TFAAVERGIFVATSAGNNGPQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSS 370
+FAA+++G+FV TS GN+GP +L NGAPW++ V AGT+ R F GT+T N VS S
Sbjct: 309 SFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFPS 368
Query: 371 LFPLNTTMSLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALG 430
LFP P+ + G +N K + +IVVC N+ ++ S++ +++ A
Sbjct: 369 LFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGAAAV 428
Query: 431 IFISN-ISDWDNLIQTPFPSIFLNPYHGSIIKDYIHKS-SDPKAEVNFHKTILGTKPAPI 490
+ I++ + + + I+ FP F+ H I+ Y + ++ A++ F KT++GTKPAP
Sbjct: 429 VLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKPAPE 488
Query: 491 VARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSC 550
V YSSRGP S P +LKPDI+APG IL++WP + P++S FN+++GTSM+
Sbjct: 489 VGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTSMAA 548
Query: 551 PHAAGVAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHV 610
PH AGVAAL+K HP WSP+AI+SA+MTTA +DN PLA+G+GHV
Sbjct: 549 PHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGAGHV 608
Query: 611 NPNKAIDPDLIYDVRIQDYVNVLC-ALNYTENQIRIITRSDSND-CENPSLDLNYPSFII 670
+ NK ++P LIYD QD++N LC + I IITRS+ +D C+ PS LNYPS I
Sbjct: 609 STNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPSIIA 668
Query: 671 IVNS--SDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQ 730
S S PK FKRTLT +GE + +Y ++RG+KG V V+PK L F KN+
Sbjct: 669 YFTSDQSSPKI--------FKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 728
Query: 731 KLSFELKI-AGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTRV 757
KLS+ +++ + + +VV+G +SWV+ S VVA + V
Sbjct: 729 KLSYTVRLESPRGLQENVVYGLVSWVDEDEAEFEVSCSVVATSLV 733
BLAST of PI0028598 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 515.8 bits (1327), Expect = 6.0e-146
Identity = 302/756 (39.95%), Postives = 435/756 (57.54%), Query Frame = 0
Query: 22 TETHNYIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLIHTYNHAISGFC 81
T YI+ +N + P+ F +HH WY++ ++S S L++TY + GF
Sbjct: 25 TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------ESSLLYTYTTSFHGFS 84
Query: 82 ASLTPSQLEALKNSPGYLTSVLDSSVH-VDTTHSSHFLGLNSNSGLLPISKYGSDVIIGF 141
A L ++ ++L +S + + + ++ + TT + FLGLNS G+ + + VIIG
Sbjct: 85 AYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGV 144
Query: 142 VDTGIWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMARSPNA 201
+DTG+WP+S SF+D M EIPS+WKGECE+ + F+ CNKKLIGAR F+KG S
Sbjct: 145 LDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGG 204
Query: 202 TISMN---STRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEE 261
S S RD GHGTHTSTTAAGS V+ ASF GY GTARG+A RARVA YK W
Sbjct: 205 FSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWST 264
Query: 262 GNSVSDVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATFAAVERGIFVATSAGNNG 321
G SD++AA+D+AI DGVDV+SLS+G P Y+D +AI F+A+ERG+FV+ SAGN+G
Sbjct: 265 GCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSG 324
Query: 322 PQLETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLFPLNTTMSLSPLPIVFMG 381
P ++ N APWV+ V AGT+DRDF L NG + G SL+ M PL +V+
Sbjct: 325 PTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKPLELVYNK 384
Query: 382 GCQNLKKLKRIGY--------KIVVCEDNDGYSLTSQVDNVQTAQVALGIFISN-ISDWD 441
G + L G KIVVC+ G + + V LG+ ++N + +
Sbjct: 385 GNSSSSNLCLPGSLDSSIVRGKIVVCD--RGVNARVEKGAVVRDAGGLGMIMANTAASGE 444
Query: 442 NLIQTP--FPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARYSSRGPS 501
L+ P+I + G ++++Y+ S P A + F T+L KP+P+VA +SSRGP+
Sbjct: 445 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPN 504
Query: 502 QSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAAGVAALL 561
P +LKPD++ PG ILA W + ++ ++FN++SGTSMSCPH +G+A LL
Sbjct: 505 TVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLL 564
Query: 562 KGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKAIDPDL 621
K AHP WSP+AI+SA+MTTA V+DNT + D + N + P A GSGHV+P KA+ P L
Sbjct: 565 KAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVDPQKALSPGL 624
Query: 622 IYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSLD---LNYPSFIIIVNSSDPKT 681
+YD+ ++Y+ LC+L+YT + I I + S +C D LNYPSF ++
Sbjct: 625 VYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFG------ 684
Query: 682 GKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFK----RKNQKLSFELK 741
GKR + + R +T +G + Y+ + G + VKP L FK +K ++F K
Sbjct: 685 GKRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSK 744
Query: 742 IAGSVTESDVVFGYLSWVEVGGGHIVQSPIVVAGTR 756
S+T + FG ++W H V+SP+ + R
Sbjct: 745 KGVSMT-NKAEFGSITW--SNPQHEVRSPVAFSWNR 753
BLAST of PI0028598 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 510.4 bits (1313), Expect = 2.5e-144
Identity = 310/760 (40.79%), Postives = 438/760 (57.63%), Query Frame = 0
Query: 27 YIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
YIVH++ A P F +H WY+ SS +S +S P +IHTY+ GF A LT
Sbjct: 28 YIVHVDHEAKPSIFPTHFHWYT--------SSLASLTSSPPSIIHTYDTVFHGFSARLTS 87
Query: 87 SQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNS--NSGLLPISKYGSDVIIGFVDTG 146
L + P ++ + + H+ TT S FLGL S +GLL S +GSD++IG +DTG
Sbjct: 88 QDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTG 147
Query: 147 IWPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMARS--PNATI 206
+WP+ SF+D + +P +WKG+C S F S CN+KL+GARFF G A + N T
Sbjct: 148 VWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETT 207
Query: 207 SMNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVS 266
S RD+ GHGTHT++ +AG YV AS GY G A G+AP+AR+A YK W G S
Sbjct: 208 EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDS 267
Query: 267 DVIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATFAAVERGIFVATSAGNNGPQLET 326
D++AA D A++DGVDVISLS+G VP Y D +AI F A++RGIFV+ SAGN GP T
Sbjct: 268 DILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALT 327
Query: 327 LHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLFPLNTTMSLSPLPIVF----MGG 386
+ N APW+ V AGT+DRDF V L NG + G S++ P+V+ +GG
Sbjct: 328 VTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGG 387
Query: 387 CQNLKKL--------KRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIFISN-ISDWDN 446
L + KIV+C+ G + + + LG+ I+N + D +
Sbjct: 388 DGYSSSLCLEGSLDPNLVKGKIVLCD--RGINSRATKGEIVRKNGGLGMIIANGVFDGEG 447
Query: 447 LIQT--PFPSIFLNPYHGSIIKDYIHKSS------DPKAEVNFHKTILGTKPAPIVARYS 506
L+ P+ + G I+ YI +SS P A + F T LG +PAP+VA +S
Sbjct: 448 LVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFS 507
Query: 507 SRGPSQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAAG 566
+RGP+ P +LKPD++APG ILA+WP + V S ++FN++SGTSM+CPH +G
Sbjct: 508 ARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSG 567
Query: 567 VAALLKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKA 626
+AALLK AHP WSPAAIRSA++TTA VDN+ + D N ++ + GSGHV+P KA
Sbjct: 568 LAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSGHVHPTKA 627
Query: 627 IDPDLIYDVRIQDYVNVLCALNYTENQIRIITRSDSNDCENPSL-----DLNYPSFIIIV 686
+DP L+YD+ DY+N LC NYT I ITR + DC+ +LNYPSF ++
Sbjct: 628 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNYPSFSVVF 687
Query: 687 NSSDPKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSF 746
+ G+ K+ F RT+T +G++ + YE K+R +G V V+P+ L F+R QKLSF
Sbjct: 688 Q----QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSF 747
Query: 747 ELKIAGSVTE-----SDVVFGYLSWVEVGGGHIVQSPIVV 752
+++ + + ++V G++ W + G V SP+VV
Sbjct: 748 VVRVKTTEVKLSPGATNVETGHIVWSD--GKRNVTSPLVV 769
BLAST of PI0028598 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 500.7 bits (1288), Expect = 2.0e-141
Identity = 299/754 (39.66%), Postives = 427/754 (56.63%), Query Frame = 0
Query: 27 YIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
+I ++ +MP F +H+ WYS + S+++H Y+ GF A +TP
Sbjct: 28 FIFRIDGGSMPSIFPTHYHWYSTEFAE------------ESRIVHVYHTVFHGFSAVVTP 87
Query: 87 SQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNSNSGLLPISKYGSDVIIGFVDTGIW 146
+ + L+N P L D + TT S FLGL + GL S YGSDVIIG DTGIW
Sbjct: 88 DEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGVFDTGIW 147
Query: 147 PDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMAR---SPNATIS 206
P+ SF+D + IP RW+G CE+ F+ CN+K+IGARFF KG A N T+
Sbjct: 148 PERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVE 207
Query: 207 MNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSV-S 266
S RD GHGTHTS+TAAG + +AS GY G A+GVAP+AR+A YK W++ + S
Sbjct: 208 FLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDS 267
Query: 267 DVIAAIDQAISDGVDVISLSI-GLDGV--PLYKDPVAIATFAAVERGIFVATSAGNNGPQ 326
D++AA D A+ DGVDVIS+SI G DG+ P Y DP+AI ++ A +GIFV++SAGN GP
Sbjct: 268 DILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPN 327
Query: 327 LETLHNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLF---PLNTTMSLSPLPIVFM 386
++ N APWV V A T+DR+F L +G + G SL+ PLN M P+V+
Sbjct: 328 GMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM----FPVVYP 387
Query: 387 GG---------CQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIFISNISDW 446
G +N K++ KIV+C+ + + + V + I + S+
Sbjct: 388 GKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGM-ILANGASNG 447
Query: 447 DNLIQTP--FPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARYSSRGP 506
+ L+ P+ + G IK Y +P A ++F TI+G KPAP++A +S RGP
Sbjct: 448 EGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGP 507
Query: 507 SQSCPFVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAAGVAAL 566
+ P +LKPD++APG ILA+W V + S P ++FN++SGTSM+CPH +G AAL
Sbjct: 508 NGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAAL 567
Query: 567 LKGAHPLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKAIDPD 626
LK AHP WSPA IRSAMMTT ++VDN+ S+ D + K ATP GSGH+N +A++P
Sbjct: 568 LKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGHLNLGRAMNPG 627
Query: 627 LIYDVRIQDYVNVLCALNYTENQIRIITRSDSN--DCENPSL-DLNYPSFIIIVNSSDPK 686
L+YD+ DY+ LC++ Y I++ITR+ PS +LNYPS + P
Sbjct: 628 LVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVF----PT 687
Query: 687 TGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFVVRVKPKILKFKRKNQKLSFELKIAG 746
+ + RT T +G+ A Y A++ +G V VKP L F ++ S+ + +
Sbjct: 688 NRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTV 747
Query: 747 S-----VTESDVVFGYLSWVEVGGGHIVQSPIVV 752
+ + E+ VFG ++W + GG H+V+SPIVV
Sbjct: 748 NTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVV 758
BLAST of PI0028598 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 500.4 bits (1287), Expect = 2.6e-141
Identity = 302/750 (40.27%), Postives = 431/750 (57.47%), Query Frame = 0
Query: 27 YIVHMNSAAMPKPFASHHSWYSATVSSVLHSSSSSSSSFPSKLIHTYNHAISGFCASLTP 86
YIVHM + MP F H +WY +++ S+ S ++L++TY +AI GF LT
Sbjct: 32 YIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---------AELLYTYENAIHGFSTRLTQ 91
Query: 87 SQLEALKNSPGYLTSVLDSSVHVDTTHSSHFLGLNSNSG-LLPISKYGSDVIIGFVDTGI 146
+ ++L PG ++ + + + TT + FLGL+ ++ L P + SDV++G +DTG+
Sbjct: 92 EEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGV 151
Query: 147 WPDSESFNDNAMSEIPSRWKGECENSTHFNVSFCNKKLIGARFFNKGLMARSP--NATIS 206
WP+S+S++D IPS WKG CE T+F S CN+KLIGARFF +G + + +
Sbjct: 152 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKE 211
Query: 207 MNSTRDTIGHGTHTSTTAAGSYVKEASFFGYGRGTARGVAPRARVAIYKAIWEEGNSVSD 266
S RD GHGTHTS+TAAGS V+ AS GY GTARG+APRARVA+YK W G SD
Sbjct: 212 SRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSD 271
Query: 267 VIAAIDQAISDGVDVISLSIGLDGVPLYKDPVAIATFAAVERGIFVATSAGNNGPQLETL 326
++AAID+AI+D V+V+S+S+G Y+D VAI FAA+ERGI V+ SAGN GP +L
Sbjct: 272 ILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSL 331
Query: 327 HNGAPWVLNVVAGTMDRDFGGTVTLSNGVSVLGSSLFPLNTTMSLSPLPIVFMGGCQNLK 386
N APW+ V AGT+DRDF L NG + G SLF + LP ++ G N
Sbjct: 332 SNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK-GEALPDKLLPFIYAGNASNAT 391
Query: 387 K----------LKRIGYKIVVCEDNDGYSLTSQVDNVQTAQVALGIFISN-ISDWDNLIQ 446
+++ KIV+C+ G + Q +V A +G+ ++N ++ + L+
Sbjct: 392 NGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILANTAANGEELVA 451
Query: 447 TP--FPSIFLNPYHGSIIKDYIHKSSDPKAEVNFHKTILGTKPAPIVARYSSRGPSQSCP 506
P+ + G II+ Y+ +P A ++ T++G KP+P+VA +SSRGP+ P
Sbjct: 452 DAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITP 511
Query: 507 FVLKPDIMAPGDAILASWPQNVAAMDVNSTPIYSKFNVISGTSMSCPHAAGVAALLKGAH 566
+LKPD++APG ILA+W + S +FN+ISGTSMSCPH +G+AALLK H
Sbjct: 512 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 571
Query: 567 PLWSPAAIRSAMMTTADVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKAIDPDLIYDV 626
P WSPAAIRSA+MTTA + DI K +TP G+GHV+P A +P LIYD+
Sbjct: 572 PEWSPAAIRSALMTTAYKTYKDGKPLLDIAT-GKPSTPFDHGAGHVSPTTATNPGLIYDL 631
Query: 627 RIQDYVNVLCALNYTENQIRIITRSDSNDCENPS---LDLNYPSFIIIVNSSDPKTGKRK 686
+DY+ LCALNYT QIR ++R + + S DLNYPSF + V+ G K
Sbjct: 632 TTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDG----VGAYK 691
Query: 687 ILGEFKRTLTKIGENRATYEAKLRG-MKGFVVRVKPKILKFKRKNQKLSFEL--KIAGSV 746
+ RT+T +G TY K+ G + V+P +L FK N+K S+ + + S
Sbjct: 692 ----YTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSK 751
Query: 747 TESDVVFGYLSWVEVGGGHIVQSPIVVAGT 755
FG + W + G H+V SP+ ++ T
Sbjct: 752 PSGSNSFGSIEWSD--GKHVVGSPVAISWT 757
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O82777 | 6.8e-187 | 47.72 | Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1 | [more] |
| Q9FHA4 | 1.2e-167 | 44.18 | Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 S... | [more] |
| Q9ZUF6 | 8.4e-145 | 39.95 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
| Q9LUM3 | 3.5e-143 | 40.79 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
| O49607 | 2.8e-140 | 39.66 | Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5A7V589 | 0.0e+00 | 92.27 | Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3BBC3 | 0.0e+00 | 92.27 | subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=... | [more] |
| A0A0A0LKL6 | 0.0e+00 | 90.87 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1 | [more] |
| A0A6J1K9W2 | 0.0e+00 | 79.42 | subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3... | [more] |
| A0A6J1HH07 | 0.0e+00 | 78.36 | subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_008444560.1 | 0.0e+00 | 92.27 | PREDICTED: subtilisin-like protease SBT1.9 [Cucumis melo] >KAA0060951.1 subtilis... | [more] |
| XP_004143027.3 | 0.0e+00 | 90.87 | subtilisin-like protease SBT3 [Cucumis sativus] >KGN62450.1 hypothetical protein... | [more] |
| XP_038885776.1 | 0.0e+00 | 85.02 | subtilisin-like protease SBT3 [Benincasa hispida] | [more] |
| XP_022996869.1 | 0.0e+00 | 79.42 | subtilisin-like protease SBT1.9 [Cucurbita maxima] | [more] |
| XP_023546329.1 | 0.0e+00 | 79.00 | subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo] | [more] |
