PI0028460 (gene) Melon (PI 482460) v1

Overview
NamePI0028460
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionSacchrp_dh_NADP domain-containing protein
Locationchr04: 8320360 .. 8323917 (+)
RNA-Seq ExpressionPI0028460
SyntenyPI0028460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAACCTTCCTACAATCTCATAATCTTAGGAGCCACTGGTTTCACAGGTAAATATGTAGTTCGAGAAGCTCTCAAATTCCTCGGCTCTTCCCCTCTCAAATCCCTTGCTCTTGCCGGTCGAAACCTGACCAAACTGACCGAATCCCTCAAATGGGCAGCTCATCCCCTGCCTCCGCCGCCAATTCCACTCCTACTCGCCGACGTCACCGACCCACATTCCATCCGTCGGCTCTGCAGCTACACCAAGCTGATTCTGAACTGCGTTGGACCGTTTCAGCGGTACGGGAAGGTGGTGGTTGCGGCGTGTGTGGAAACTGGGTGCGATTACTTGGACATATGTGGGGAGCCAGAGTTCATGGAGAGAATGGAGGCAGAGTATGATAAGATGGCGGTTGAGAAAGGGAGTTTGGTGGTTTCGGGATGTGGGTTTGATTCGGTTCCGGCGGAATTGGGTTTGATATTCAATTCGAGGCAGTGGGTTGGGAAATCGGCGCCGAGTTGGGTTGAAGCGTATATGAATGTGGAGTCGAAGAAAGGAATAATTTGTAACTTTGGGACATTTGAATCAGCTGTTTTGGGTGTTGCTAATGCCGAACAGTTTCGCCAATTCAGGAGATCAAGGCCCCTAAGACGCAGCCCATTGGTGAGTAACAAATTTCTATTTCTTTTTTCTTTTTCCAATAGCATGTATATACATTTAAAATGGTGAAAATTATTTGTTTTATTTATAAATAGTTTTTTTCATTTTCTTATATTTGAAATTAAACCCAAAAATTAATTTAATATTAAATTTTATACTTTTAGATACAATTTAGTTTATCAAGTTCAGTTTTGAAGCTAAAAACCACACACAAAAAAAAAAAAACATCTTTTCATGGTATTTTTTTAGTACAAACTGAAGCGAGGATAAAGTTAGAACCTCTTGGTTCTCGCTTCTTAGTATATATTAGTGTTAGTTGAGCTAAACTTTTGTTACCTCGTGATGGTTTAGAAAATGAAAAATGTAATTTTAATTATTTTTAGAATATATTTTTATTTGGTCTCTAAGCTTGGGGTTTAGTAAGTTTTTTAACTTTAAATTTTGTATCTAACATGTTCTTAAAATTTTGTCTATAATATATTTTAAAACTTTCAATTCTATATCGAACTAGGCTCTAGTATATAAATTTTATAAAAGAGTGAATTGAAAATATCTATTTGCTATATAAAATTAAATTTTATGTATAATAAAACTCTTAAGTTTAAATTTTATCTTTCATAGATGTGTTAATTTTATTTGTTAAAAAAAGTTGTTGAATAGTTGAATGATCTATTAGATACATCATTACAAAAATTAAGGATCTATTCAGTAAAAATTTTAAAATTAAAAGATTTATTAGACACTTTTTAAATTATAAAAACTTAGTTGACATAAAATTGAAATTTGGTCTTTTTAAAGTTCAAATACCTATTTGCCGAGAAATTGAAAGTTAAAAAATTAAATAGACACAAAATTAAGTTCAATTATTAAACTTATAATTTAATTTTTCTCATTTGTGAAGTTTGAATTTTGTTGATTTTACACAAACTAATACTTTCTTGTTCCTTCGATTGCACTTTTTTTTTTCCTTTTCTTTTCGGTCCACACCAATAAAAAGAAATTTACTCAATTAAAGGTAAGAAAATAGTCAATGTAGTAATAAATAAAAATTAGTATTTTTTTTCAAAGTATCAATGTTTAATATAAATGAAATAAGGTAGGAATTTGAATGGTAAACTCATCAATTTTTTTAACACACTTATACGTCAGTTAAGTTATATTTACTTTGACAATGTTTAATATATTTTTATATTAGTTAACAATTATATCACTTAAATAAAAGTAAATAAAAGTAATTTAATATTTATATATTAAAATATTGATATAACTAATATAAAGTAATTAATATTTAATAACAAAATTGTATATTAAATATATATAGTACTAACGTAGAATATTAATTGTATTAGTTGAAATTTATTAAATCTAATTAATATTTTTGTATGTAATAATTTAAAATAAAGAGATAGTTTTAAATATAGAAAAGTGAATCAAATACTTACGAAATATAGCAAAATACTATAGTCTATCTAAAATGTATCATGATGGTGATAATTTTCTATATTTGTAAATATTTTTAGAAGTTTTGTTGTTTAAAATAATTTTCTTAAGATAAATGGTAATGATTAATTGATAATAATCTATTAATCAATCATACTAGGAGTGAGCATGGGCACCAAAAAACAGATCTGAACAACTGAACTGACTGTCGAAAGTCAGCTTTTTTATAGATCAGTTCGGGTTGGTTTTTCAAACAAAATGTGAAAAATCGACCAGCACTTACGAATTAGACTGAACTCGACCGTGCTGACATATAATTAGAGTGAAAAATCACTTAAAATTTAATAGTGACAGAAAATTAGAGCAAATATAATTATAATTAATTATATTAACTCTTTTAAATTAATTAATAACATTAACAGTAAACACTACAGGTGAATATTTAGTAAGAAAATCAAAAACCAAAGTTAAAAAGAGTAAATCTCAAAACAAAACTCAAATTTTAATAATAAAAATGTAATCTTATATGAAATTTAAGAACTAAATGAAAATTACACTCAAAATTTAGCTTTTAAATTTTAGATACTAAAAATATATTTTTAGTCTACTATGTGGGCAAAAAAGTTTTTTTCCGGTAGAAAACATTAAAATACTATTGTTTCAGAAGAAGATGCATGACGTAGACTGCATATAATCACCGTTACGCTTTTAATGATTAACCTTCATCCACAATACTTTAAACTTTAAGGATTTAAGAGAAGTGTTTGATATTATTATGTTTTGTAGATTCTTAGTTGTTCAACTCCAAAAAGACCATTATTAGAGTACAAAAAAGAGTTTGGAGTGTGGAGTGTAAGAATTCCTTCAGCTGATTCAAGTGTAATTCGAAGAACACTCTTCACTCTAGCCGAAAACCCCCAAGGTCTTCCTGGTTCAAATGAGACTCCAGATGACATCGAACAAAGGAAGAAATTCTGGTCATCAGTGAAGCCAGCTCGTTTCGGAGTAAAAATCAGCATGAAATCCTTCCTCGGAATCCTACGATTCATTGTAGTCGGGATTTTTATAGGAATGCTTGCTAAAAGAGCATTCGGAAGATGGTTGCTCTTAACATTCCCTTCCCTTTTTAGTCTTGGATTATTGTGCAGTTCCAAGAACAAAGGTCCTTCAGAAGATGAAGTGAATAATGGGTCATTCAAAATGTGGTTTGTGGGACATGGGTTTGATGATAATGGGAATAATAAGGAAATAGTGACGAGAATAATGGGGCCAGAGCCTGGATATATAACCACTTCAATAATACTTATTCAATGTGCTTTTATTCTTCTAACTAAACGTCAGGTTATGCCTAAAGGAGGAGTTTTAACTCCTGGTATTGTGTTCGGACCAACTCATCTTCAACAAAGACTTCAAGATAATGGTGTATCTTTTGATGTTATTTCAAATAATAATGATGCTAAGTTGGAGAAATGA

mRNA sequence

ATGGAAGAACCTTCCTACAATCTCATAATCTTAGGAGCCACTGGTTTCACAGGTAAATATGTAGTTCGAGAAGCTCTCAAATTCCTCGGCTCTTCCCCTCTCAAATCCCTTGCTCTTGCCGGTCGAAACCTGACCAAACTGACCGAATCCCTCAAATGGGCAGCTCATCCCCTGCCTCCGCCGCCAATTCCACTCCTACTCGCCGACGTCACCGACCCACATTCCATCCGTCGGCTCTGCAGCTACACCAAGCTGATTCTGAACTGCGTTGGACCGTTTCAGCGGTACGGGAAGGTGGTGGTTGCGGCGTGTGTGGAAACTGGGTGCGATTACTTGGACATATGTGGGGAGCCAGAGTTCATGGAGAGAATGGAGGCAGAGTATGATAAGATGGCGGTTGAGAAAGGGAGTTTGGTGGTTTCGGGATGTGGTTGGGTTGAAGCGTATATGAATGTGGAGTCGAAGAAAGGAATAATTTGTAACTTTGGGACATTTGAATCAGCTGTTTTGGGTGTTGCTAATGCCGAACAGTTTCGCCAATTCAGGAGATCAAGGCCCCTAAGACGCAGCCCATTGATTCTTAGTTGTTCAACTCCAAAAAGACCATTATTAGAGTACAAAAAAGAGTTTGGAGTGTGGAGTGTAAGAATTCCTTCAGCTGATTCAAGTGTAATTCGAAGAACACTCTTCACTCTAGCCGAAAACCCCCAAGGTCTTCCTGGTTCAAATGAGACTCCAGATGACATCGAACAAAGGAAGAAATTCTGGTCATCAGTGAAGCCAGCTCGTTTCGGAGTAAAAATCAGCATGAAATCCTTCCTCGGAATCCTACGATTCATTGTAGTCGGGATTTTTATAGGAATGCTTGCTAAAAGAGCATTCGGAAGATGGTTGCTCTTAACATTCCCTTCCCTTTTTAGTCTTGGATTATTGTGCAGTTCCAAGAACAAAGGTCCTTCAGAAGATGAAGTGAATAATGGGTCATTCAAAATGTGGTTTGTGGGACATGGGTTTGATGATAATGGGAATAATAAGGAAATAGTGACGAGAATAATGGGGCCAGAGCCTGGATATATAACCACTTCAATAATACTTATTCAATGTGCTTTTATTCTTCTAACTAAACGTCAGGTTATGCCTAAAGGAGGAGTTTTAACTCCTGGTATTGTGTTCGGACCAACTCATCTTCAACAAAGACTTCAAGATAATGGTGTATCTTTTGATGTTATTTCAAATAATAATGATGCTAAGTTGGAGAAATGA

Coding sequence (CDS)

ATGGAAGAACCTTCCTACAATCTCATAATCTTAGGAGCCACTGGTTTCACAGGTAAATATGTAGTTCGAGAAGCTCTCAAATTCCTCGGCTCTTCCCCTCTCAAATCCCTTGCTCTTGCCGGTCGAAACCTGACCAAACTGACCGAATCCCTCAAATGGGCAGCTCATCCCCTGCCTCCGCCGCCAATTCCACTCCTACTCGCCGACGTCACCGACCCACATTCCATCCGTCGGCTCTGCAGCTACACCAAGCTGATTCTGAACTGCGTTGGACCGTTTCAGCGGTACGGGAAGGTGGTGGTTGCGGCGTGTGTGGAAACTGGGTGCGATTACTTGGACATATGTGGGGAGCCAGAGTTCATGGAGAGAATGGAGGCAGAGTATGATAAGATGGCGGTTGAGAAAGGGAGTTTGGTGGTTTCGGGATGTGGTTGGGTTGAAGCGTATATGAATGTGGAGTCGAAGAAAGGAATAATTTGTAACTTTGGGACATTTGAATCAGCTGTTTTGGGTGTTGCTAATGCCGAACAGTTTCGCCAATTCAGGAGATCAAGGCCCCTAAGACGCAGCCCATTGATTCTTAGTTGTTCAACTCCAAAAAGACCATTATTAGAGTACAAAAAAGAGTTTGGAGTGTGGAGTGTAAGAATTCCTTCAGCTGATTCAAGTGTAATTCGAAGAACACTCTTCACTCTAGCCGAAAACCCCCAAGGTCTTCCTGGTTCAAATGAGACTCCAGATGACATCGAACAAAGGAAGAAATTCTGGTCATCAGTGAAGCCAGCTCGTTTCGGAGTAAAAATCAGCATGAAATCCTTCCTCGGAATCCTACGATTCATTGTAGTCGGGATTTTTATAGGAATGCTTGCTAAAAGAGCATTCGGAAGATGGTTGCTCTTAACATTCCCTTCCCTTTTTAGTCTTGGATTATTGTGCAGTTCCAAGAACAAAGGTCCTTCAGAAGATGAAGTGAATAATGGGTCATTCAAAATGTGGTTTGTGGGACATGGGTTTGATGATAATGGGAATAATAAGGAAATAGTGACGAGAATAATGGGGCCAGAGCCTGGATATATAACCACTTCAATAATACTTATTCAATGTGCTTTTATTCTTCTAACTAAACGTCAGGTTATGCCTAAAGGAGGAGTTTTAACTCCTGGTATTGTGTTCGGACCAACTCATCTTCAACAAAGACTTCAAGATAATGGTGTATCTTTTGATGTTATTTCAAATAATAATGATGCTAAGTTGGAGAAATGA

Protein sequence

MEEPSYNLIILGATGFTGKYVVREALKFLGSSPLKSLALAGRNLTKLTESLKWAAHPLPPPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPEFMERMEAEYDKMAVEKGSLVVSGCGWVEAYMNVESKKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVGHGFDDNGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGIVFGPTHLQQRLQDNGVSFDVISNNNDAKLEK
Homology
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BLAST of PI0028460 vs. ExPASy Swiss-Prot
Match: Q8LGI2 (Probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Arabidopsis thaliana OX=3702 GN=At5g39410 PE=1 SV=2)

HSP 1 Score: 498.8 bits (1283), Expect = 5.9e-140
Identity = 256/446 (57.40%), Postives = 325/446 (72.87%), Query Frame = 0 

Query: 3   EPSYNLIILGATGFTGKYVVREALKFL---GSSPLKSLALAGRNLTKLTESLKWAAHPLP 62
           EP Y+++ILGA+GFTGKYVVREALKFL    SSPLKSLALAGRN T+LT+SL+WAA P P
Sbjct: 8   EPVYDMVILGASGFTGKYVVREALKFLQTPSSSPLKSLALAGRNPTRLTQSLEWAARPNP 67

Query: 63  PP-PIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEP 122
           PP  + +L AD +DP S+RRLC+ TKLILNCVGPF+ +G  VV+AC ++GCDYLDI GEP
Sbjct: 68  PPSSVAILTADTSDPDSLRRLCTQTKLILNCVGPFRIHGDPVVSACADSGCDYLDISGEP 127

Query: 123 EFMERMEAEYDKMAVEKGSLVVSGCGW------------------------VEAYMNVES 182
           EFMERMEA Y   A E GSL+VS CG+                        +EAY+++ES
Sbjct: 128 EFMERMEANYHDRAEETGSLIVSACGFDSIPAELGLLFNAKQWVSPSVPNQIEAYLSLES 187

Query: 183 KKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTP-KRPLLEYKKEFGVW 242
            K I  NFGT+ESAVLGVANAE+ ++ RRSRP R  P I  C  P K P LE +K  G+W
Sbjct: 188 DKKIAGNFGTYESAVLGVANAEKLKELRRSRPRRPRPTI--CGPPAKGPTLENQKTIGLW 247

Query: 243 SVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSF 302
           ++++PSAD+ V+RRTL TL E P GLPG NE+P+ I++R+ FWSS+KPA FGVKI+ KS 
Sbjct: 248 ALKLPSADAVVVRRTLTTLTEKPHGLPGINESPEQIQKREAFWSSIKPAHFGVKITSKSL 307

Query: 303 LGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWF 362
            GI R++ +G+ +G+L+K +FGRWLLL FPS+FSLG     + KGPSE+EV + +FKMWF
Sbjct: 308 FGIFRYVTLGVSLGLLSKFSFGRWLLLKFPSVFSLGWF---QKKGPSEEEVESATFKMWF 367

Query: 363 VGHGFDD-----NGNNK---EIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVL 412
           +G G+ +      G  K   EI+TRI GPE GYITT I L+QC  I+L +R+ + KGGV 
Sbjct: 368 IGRGYSEESLASQGETKPDLEIITRISGPEIGYITTPITLVQCGLIVLGQRESLVKGGVY 427

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BLAST of PI0028460 vs. ExPASy Swiss-Prot
Match: Q8R127 (Saccharopine dehydrogenase-like oxidoreductase OS=Mus musculus OX=10090 GN=Sccpdh PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.4e-56
Identity = 137/449 (30.51%), Postives = 228/449 (50.78%), Query Frame = 0 

Query: 2   EEPSYNLIILGATGFTGKYVVREALKFLGSSPLKS---LALAGRNLTKLTESLKWAAHPL 61
           E+  ++L++ GA+GFTG++V  E  +   +S   S    A+AGR+  KL + L+ AA  L
Sbjct: 4   EQRPFHLVVFGASGFTGQFVTEEVAREQIASEQSSRLPWAVAGRSKEKLQQVLEKAAQKL 63

Query: 62  PPP----PIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDI 121
             P     + +++ D+++P S+  +    KL+LNCVGP++ YG+ VV AC+E G   +DI
Sbjct: 64  GRPSLSSEVGVIICDISNPASLDEMAKQAKLVLNCVGPYRFYGEPVVKACIENGTSCIDI 123

Query: 122 CGEPEFMERMEAEYDKMAVEKGSLVVSGCGW----------------------VEAYMNV 181
           CGEP+F+E M A+Y + A EKG  ++   G+                      VE+++ +
Sbjct: 124 CGEPQFLELMHAKYHEKAAEKGVYIIGSSGFDSIPADLGVLYTRNQMNGTLTAVESFLTI 183

Query: 182 ES-KKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFG 241
            +  +G+  + GT++SA+ G  +    R+ R    L+  P I+     +R  + Y +E  
Sbjct: 184 NTGPEGLCIHDGTWKSAIYGFGDKGSLRKLRSVSCLKPVP-IVGTKLKRRWPVSYCRELN 243

Query: 242 VWSVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMK 301
            +S+    +D SV++RT   L EN +                       P ++   +++ 
Sbjct: 244 SYSIPFLGSDISVVKRTQRYLHENLED---------------------SPVQYAAYVTVG 303

Query: 302 SFLGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKM 361
               +++ +  G+F     K + GR LL+ FP LFS G       +GP++ +++  SF M
Sbjct: 304 GITSVIKLMFAGLFFLFFVKFSIGRQLLIKFPWLFSFGYF---SKQGPTQKQMDETSFTM 363

Query: 362 WFVGHGF------DDNGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPK-GGV 414
            F G G+      + N  N  I T++ GPE GY+ T I ++Q A   L+    +PK GGV
Sbjct: 364 TFFGQGYSHGTCVEKNKPNIRICTQVKGPEAGYVATPIAMVQAAMTFLSDASDLPKGGGV 423

[Close]
BLAST of PI0028460 vs. ExPASy Swiss-Prot
Match: Q3T067 (Saccharopine dehydrogenase-like oxidoreductase OS=Bos taurus OX=9913 GN=SCCPDH PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.4e-55
Identity = 138/448 (30.80%), Postives = 225/448 (50.22%), Query Frame = 0 

Query: 2   EEPSYNLIILGATGFTGKYVVREALKFLGSSPLKS---LALAGRNLTKLTESLKWAAHPL 61
           ++  ++L++ GA+GFTG++V  E  +   S    S    A+AGR+  KL   L+ AA  L
Sbjct: 4   QQRPFHLVVFGASGFTGQFVTEEVAREQVSPERTSHLPWAVAGRSREKLLRVLERAAMKL 63

Query: 62  PPP----PIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDI 121
             P     + +++ D+T+P S+  +     ++LNCVGP++ YG+ V+ AC+E G   +DI
Sbjct: 64  GRPTLSSEVGIIICDITNPASLDEMAKQATVVLNCVGPYRFYGEPVIKACIENGTSCIDI 123

Query: 122 CGEPEFMERMEAEYDKMAVEKGSLVVSGCGW----------------------VEAYMNV 181
            GEP+F+E M  +Y + A EKG  ++   G+                      VE+++ +
Sbjct: 124 SGEPQFLELMYWKYHEKAAEKGVYIIGSSGFDSIPADLGVIYTRNKMNGTLTAVESFLTI 183

Query: 182 ES-KKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFG 241
            S  +G+  + GT++SAV G  +    ++ R    ++  P I+     +R  + Y +E  
Sbjct: 184 SSGPEGLCVHDGTWKSAVYGFGDKSNLKKLRNESDMKPVP-IVGPKLKRRWPISYCRELN 243

Query: 242 VWSVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMK 301
            +S+    AD SV++RT   L EN             +EQ         P ++   I++ 
Sbjct: 244 SYSIPFLGADVSVVKRTQRYLHEN-------------LEQ--------SPVQYAAYINVG 303

Query: 302 SFLGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKM 361
               +++ +  G+F     +   GR LL+ F  LFS G       +GP++ +++  SF M
Sbjct: 304 GITSVIKLMFAGLFFLFFVRFGIGRQLLIKFTWLFSFGYF---SKQGPTQKQIDASSFTM 363

Query: 362 WFVGHGFDD------NGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPK-GGV 413
            F G GF        N  N  I T++ GPE GY++TSI ++Q A ILL     +PK GGV
Sbjct: 364 TFFGQGFSQGVSPVKNKPNIRICTQVKGPEAGYVSTSIAMVQAAMILLNDASDLPKAGGV 423

[Close]
BLAST of PI0028460 vs. ExPASy Swiss-Prot
Match: Q6AY30 (Saccharopine dehydrogenase-like oxidoreductase OS=Rattus norvegicus OX=10116 GN=Sccpdh PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.5e-55
Identity = 136/449 (30.29%), Postives = 224/449 (49.89%), Query Frame = 0 

Query: 2   EEPSYNLIILGATGFTGKYVVREALKFLGSSPLKS---LALAGRNLTKLTESLKWAAHPL 61
           E+  + L++ GA+GFTG++V  E  +   +S   S    A+AGR+  KL + L+ AA  L
Sbjct: 4   EQRPFQLVVFGASGFTGQFVTEEVAREQMASEQSSRLPWAVAGRSKEKLQQVLEKAAQKL 63

Query: 62  P----PPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDI 121
                   + +++ D+++P S+  +     L+LNCVGP++ YG+ VV AC+E G   +DI
Sbjct: 64  GRATLSSEVGIIICDISNPASLDEMAKKATLVLNCVGPYRFYGEPVVKACIENGTSCIDI 123

Query: 122 CGEPEFMERMEAEYDKMAVEKGSLVVSGCGW----------------------VEAYMNV 181
           CGEP+F+E M  +Y + A EKG  ++   G+                      VE+++ +
Sbjct: 124 CGEPQFLELMHVKYHEKAAEKGVYIIGSSGFDSIPADLGVLYTRNQMNGTLTAVESFLTI 183

Query: 182 ES-KKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFG 241
            S  +G+  + GT++SA+ G  +    R+ R    L+  P+I S    +R  + Y +E  
Sbjct: 184 NSGPEGLCIHDGTWKSAIYGFGDKGSLRKLRSVSNLKPVPVIGS-KLKRRWPVSYCRELN 243

Query: 242 VWSVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMK 301
            +++    +D SV++RT   L EN +                       P ++   I++ 
Sbjct: 244 SYAIPFLGSDMSVVKRTQRYLHENLED---------------------SPVQYAAYITVG 303

Query: 302 SFLGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKM 361
               +++ +  G+F     K + GR LL+ FP LFS G       +GP++ +++  SF M
Sbjct: 304 GITSVIKLMFAGLFFLFFVKFSIGRQLLVKFPWLFSFGYF---SKRGPTQKQMDESSFTM 363

Query: 362 WFVGHGF------DDNGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPK-GGV 414
            F G G+      + N  N  I T++ GPE GY+ T I ++Q A   L     +PK GGV
Sbjct: 364 TFFGQGYSHGVSAEKNKPNIRICTQVKGPEAGYVATPIAMVQAAVTFLNDASDLPKGGGV 423

[Close]
BLAST of PI0028460 vs. ExPASy Swiss-Prot
Match: Q8NBX0 (Saccharopine dehydrogenase-like oxidoreductase OS=Homo sapiens OX=9606 GN=SCCPDH PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.1e-53
Identity = 133/449 (29.62%), Postives = 224/449 (49.89%), Query Frame = 0 

Query: 2   EEPSYNLIILGATGFTGKYVVREALKFLGSSPLKS---LALAGRNLTKLTESLKWAAHPL 61
           E+  ++L++ GA+GFTG++V  E  +        S    A+AGR+  KL   L+ AA  L
Sbjct: 4   EQRPFHLVVFGASGFTGQFVTEEVAREQVDPERSSRLPWAVAGRSREKLQRVLEKAALKL 63

Query: 62  PPP----PIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDI 121
             P     + +++ D+ +P S+  +     ++LNCVGP++ YG+ V+ AC+E G   +DI
Sbjct: 64  GRPTLSSEVGIIICDIANPASLDEMAKQATVVLNCVGPYRFYGEPVIKACIENGASCIDI 123

Query: 122 CGEPEFMERMEAEYDKMAVEKGSLVVSGCGW----------------------VEAYMNV 181
            GEP+F+E M+ +Y + A +KG  ++   G+                      VE+++ +
Sbjct: 124 SGEPQFLELMQLKYHEKAADKGVYIIGSSGFDSIPADLGVIYTRNKMNGTLTAVESFLTI 183

Query: 182 ES-KKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFG 241
            S  +G+  + GT++SA+ G  +    R+ R    L+  PLI      +R  + Y +E  
Sbjct: 184 HSGPEGLSIHDGTWKSAIYGFGDQSNLRKLRNVSNLKPVPLI-GPKLKRRWPISYCRELK 243

Query: 242 VWSVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMK 301
            +S+    +D SV+RRT   L EN             +E+         P ++   +++ 
Sbjct: 244 GYSIPFMGSDVSVVRRTQRYLYEN-------------LEE--------SPVQYAAYVTVG 303

Query: 302 SFLGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKM 361
               +++ +  G+F     +   GR LL+ FP  FS G       +GP++ +++  SF +
Sbjct: 304 GITSVIKLMFAGLFFLFFVRFGIGRQLLIKFPWFFSFGYF---SKQGPTQKQIDAASFTL 363

Query: 362 WFVGHGF------DDNGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPK-GGV 414
            F G G+      D N  N +I T++ GPE GY+ T I ++Q A  LL+    +PK GGV
Sbjct: 364 TFFGQGYSQGTGTDKNKPNIKICTQVKGPEAGYVATPIAMVQAAMTLLSDASHLPKAGGV 423

[Close]
BLAST of PI0028460 vs. ExPASy TrEMBL
Match: A0A0A0LCQ2 (Sacchrp_dh_NADP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824730 PE=4 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 7.4e-210
Identity = 369/413 (89.35%), Postives = 383/413 (92.74%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFLGSSPLKSLALAGRNLTKLTESLKWAAHPLPP 60
           MEEP Y+L+ILGATGFTGKYVVREALKFL  SPLKSLALAGRNLTKL ESLKWAAHPLPP
Sbjct: 1   MEEPPYDLVILGATGFTGKYVVREALKFLNISPLKSLALAGRNLTKLNESLKWAAHPLPP 60

Query: 61  PPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPEF 120
           PPIPLLLADVTDPHSIRRLCSYTKLILNCVGPF+RYGK VV ACVETGCDYLDICGEPEF
Sbjct: 61  PPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFRRYGKAVVEACVETGCDYLDICGEPEF 120

Query: 121 MERMEAEYDKMAVEKGSLVVSGCGWVEAYMNVESKKGIICNFGTFESAVLGVANAEQFRQ 180
           MERMEAEYDKMAVEKGSLVVSGCG      NVESK GI+CNFG+FESAVLGVANA + RQ
Sbjct: 121 MERMEAEYDKMAVEKGSLVVSGCG----VQNVESKNGIVCNFGSFESAVLGVANAGELRQ 180

Query: 181 FRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSVRIPSADSSVIRRTLFTLAENPQGLP 240
           FRRSRP RRSPLI+  STPK PLLEYKK+FGVWSVRIPSADSSVIRRTLFTLAENPQGL 
Sbjct: 181 FRRSRPPRRSPLIVGRSTPKGPLLEYKKQFGVWSVRIPSADSSVIRRTLFTLAENPQGLL 240

Query: 241 GSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLGILRFIVVGIFIGMLAKRAFGRWLLL 300
           GSNET DDIEQRKKFWSSVKPARFGVKI MKSFLGILRFIV GIFIGMLAK+AFGRWLLL
Sbjct: 241 GSNETLDDIEQRKKFWSSVKPARFGVKIGMKSFLGILRFIVFGIFIGMLAKKAFGRWLLL 300

Query: 301 TFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVGHGFDDNGNNKEIVTRIMGPEPGYIT 360
           TFPS+FSLGLL     K PSEDEVNN SFKMWFVGHGFDDNGN+KEIVTRIMGPEPGYIT
Sbjct: 301 TFPSIFSLGLL---NKKSPSEDEVNNVSFKMWFVGHGFDDNGNDKEIVTRIMGPEPGYIT 360

Query: 361 TSIILIQCAFILLTKRQVMPKGGVLTPGIVFGPTHLQQRLQDNGVSFDVISNN 414
           TSIILIQCAFILLTKRQV+PKGGVLTPGIVFGPTHLQQ+LQDNG+SFDVISNN
Sbjct: 361 TSIILIQCAFILLTKRQVLPKGGVLTPGIVFGPTHLQQKLQDNGISFDVISNN 406

[Close]
BLAST of PI0028460 vs. ExPASy TrEMBL
Match: A0A5D3DPV4 (Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007630 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.8e-174
Identity = 317/447 (70.92%), Postives = 351/447 (78.52%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFL---GSSPLKSLALAGRNLTKLTESLKWAAHP 60
           +  P Y+LIILGATGFTGKY+VREALKFL    SSPLKSLALAGR+  KLT +LKWAAHP
Sbjct: 10  LSTPPYDLIILGATGFTGKYIVREALKFLNPSSSSPLKSLALAGRDHAKLTNALKWAAHP 69

Query: 61  LPPPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGE 120
           LP PPIPLL++D+T P SIR LC+ TKLILNCVGPF+RYG+ VVAACVETGCDYLDICGE
Sbjct: 70  LPSPPIPLLISDITKPQSIRHLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGE 129

Query: 121 PEFMERMEAEYDKMAVEKGSLVVSGCG------------------------WVEAYMNVE 180
           PEFMERMEA+YD  AVE GSLVVSGCG                        WVEAY+NVE
Sbjct: 130 PEFMERMEAKYDGRAVETGSLVVSGCGFDSIPAELGLIFNLRHWVGKSTPSWVEAYVNVE 189

Query: 181 SKKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVW 240
             +G+  NFGT+ESAVLGVANA+   Q RRSRP RR P     + PK PL+E+KKE G+W
Sbjct: 190 CSEGMTYNFGTYESAVLGVANADALVQLRRSRPPRRRPKFTGGAPPKGPLIEHKKEIGLW 249

Query: 241 SVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSF 300
           +VRIPSADSSV+RRTL TLA+NPQ           I+    FWSSVKPARF VKI  KSF
Sbjct: 250 AVRIPSADSSVVRRTLSTLAQNPQ----------QIKHMNTFWSSVKPARFIVKIGTKSF 309

Query: 301 LGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWF 360
           L ILR +  G+FIGM AK  FGRWLLLTFPS+F+LGL      KGPSEDEVNNGSFKMWF
Sbjct: 310 LEILRCVATGMFIGMFAKNPFGRWLLLTFPSIFTLGLF---NKKGPSEDEVNNGSFKMWF 369

Query: 361 VGHGFDDNGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGIVFGP 420
           VGHGFDDNGN+KEIVTRIMGPEPGYITTSIILIQCAF+LL KR VMPKGGVLTPGIVFGP
Sbjct: 370 VGHGFDDNGNDKEIVTRIMGPEPGYITTSIILIQCAFVLLAKRHVMPKGGVLTPGIVFGP 429

[Close]
BLAST of PI0028460 vs. ExPASy TrEMBL
Match: A0A1S3B7Z4 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Cucumis melo OX=3656 GN=LOC103487170 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.8e-174
Identity = 317/447 (70.92%), Postives = 351/447 (78.52%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFL---GSSPLKSLALAGRNLTKLTESLKWAAHP 60
           +  P Y+LIILGATGFTGKY+VREALKFL    SSPLKSLALAGR+  KLT +LKWAAHP
Sbjct: 10  LSTPPYDLIILGATGFTGKYIVREALKFLNPSSSSPLKSLALAGRDHAKLTNALKWAAHP 69

Query: 61  LPPPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGE 120
           LP PPIPLL++D+T P SIR LC+ TKLILNCVGPF+RYG+ VVAACVETGCDYLDICGE
Sbjct: 70  LPSPPIPLLISDITKPQSIRHLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGE 129

Query: 121 PEFMERMEAEYDKMAVEKGSLVVSGCG------------------------WVEAYMNVE 180
           PEFMERMEA+YD  AVE GSLVVSGCG                        WVEAY+NVE
Sbjct: 130 PEFMERMEAKYDGRAVETGSLVVSGCGFDSIPAELGLIFNLRHWVGKSTPSWVEAYVNVE 189

Query: 181 SKKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVW 240
             +G+  NFGT+ESAVLGVANA+   Q RRSRP RR P     + PK PL+E+KKE G+W
Sbjct: 190 CSEGMTYNFGTYESAVLGVANADALVQLRRSRPPRRRPKFTGGAPPKGPLIEHKKEIGLW 249

Query: 241 SVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSF 300
           +VRIPSADSSV+RRTL TLA+NPQ           I+    FWSSVKPARF VKI  KSF
Sbjct: 250 AVRIPSADSSVVRRTLSTLAQNPQ----------QIKHMNTFWSSVKPARFIVKIGTKSF 309

Query: 301 LGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWF 360
           L ILR +  G+FIGM AK  FGRWLLLTFPS+F+LGL      KGPSEDEVNNGSFKMWF
Sbjct: 310 LEILRCVATGMFIGMFAKNPFGRWLLLTFPSIFTLGLF---NKKGPSEDEVNNGSFKMWF 369

Query: 361 VGHGFDDNGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGIVFGP 420
           VGHGFDDNGN+KEIVTRIMGPEPGYITTSIILIQCAF+LL KR VMPKGGVLTPGIVFGP
Sbjct: 370 VGHGFDDNGNDKEIVTRIMGPEPGYITTSIILIQCAFVLLAKRHVMPKGGVLTPGIVFGP 429

[Close]
BLAST of PI0028460 vs. ExPASy TrEMBL
Match: A0A5D3DQ44 (Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007640 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.9e-166
Identity = 298/444 (67.12%), Postives = 349/444 (78.60%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFLG-SSPLKSLALAGRNLTKLTESLKWAAHPLP 60
           ME P Y+LIILGATGFTGKYVVREALKFL  SSPLKS ALAGRNLTKLT++L+WAAHP  
Sbjct: 1   MEGPPYDLIILGATGFTGKYVVREALKFLNPSSPLKSFALAGRNLTKLTQTLQWAAHPHS 60

Query: 61  PPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPE 120
           PPPIP L+AD+ DP SI RLC+ TKLILNCVGPF+RYG+ VVAACVETGCDYLDICGEPE
Sbjct: 61  PPPIPFLIADIADPQSIHRLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGEPE 120

Query: 121 FMERMEAEYDKMAVEKGSLVVSGCGW------------------------VEAYMNVESK 180
           FME+MEA Y + AV+ G+LVVS CG+                        +EAY+++ESK
Sbjct: 121 FMEKMEANYHERAVQSGALVVSACGFDSVPAELGLMFNSRQWVGQMAPNRIEAYLSLESK 180

Query: 181 KGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSV 240
           K I+ NFGTFESAVLGVANA+Q  + RRSRP +  P I     PK P +E+KKE G+W+V
Sbjct: 181 KKIVGNFGTFESAVLGVANADQLLKLRRSRPRKPRPQIPGPPPPKGPTIEHKKEIGLWAV 240

Query: 241 RIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLG 300
           R+PSADS+V+RRTL TLAENPQGLPG NE+ D+IEQRK FWSSVKPA FGVKI  KS +G
Sbjct: 241 RLPSADSTVVRRTLSTLAENPQGLPGVNESADEIEQRKTFWSSVKPAHFGVKIGTKSLIG 300

Query: 301 ILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVG 360
           ILR I VG+FIG+L K +FGRWLLLTFPS+FSLG     + KGPSE+EVN+ SFKMWF+G
Sbjct: 301 ILRIITVGMFIGLLGKTSFGRWLLLTFPSVFSLGWF---RKKGPSEEEVNSASFKMWFIG 360

Query: 361 HGFDDNGN------NKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGI 414
           HGF  + N        EIVTR+MGPE GY+TT IIL+QCA I+L+KR+ +PKGGVLTPGI
Sbjct: 361 HGFRSSNNEGNVEPEMEIVTRVMGPEIGYLTTPIILVQCALIVLSKRESLPKGGVLTPGI 420

[Close]
BLAST of PI0028460 vs. ExPASy TrEMBL
Match: A0A1S3B8K4 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Cucumis melo OX=3656 GN=LOC103486968 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.9e-166
Identity = 298/444 (67.12%), Postives = 349/444 (78.60%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFLG-SSPLKSLALAGRNLTKLTESLKWAAHPLP 60
           ME P Y+LIILGATGFTGKYVVREALKFL  SSPLKS ALAGRNLTKLT++L+WAAHP  
Sbjct: 1   MEGPPYDLIILGATGFTGKYVVREALKFLNPSSPLKSFALAGRNLTKLTQTLQWAAHPHS 60

Query: 61  PPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPE 120
           PPPIP L+AD+ DP SI RLC+ TKLILNCVGPF+RYG+ VVAACVETGCDYLDICGEPE
Sbjct: 61  PPPIPFLIADIADPQSIHRLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGEPE 120

Query: 121 FMERMEAEYDKMAVEKGSLVVSGCGW------------------------VEAYMNVESK 180
           FME+MEA Y + AV+ G+LVVS CG+                        +EAY+++ESK
Sbjct: 121 FMEKMEANYHERAVQSGALVVSACGFDSVPAELGLMFNSRQWVGQMAPNRIEAYLSLESK 180

Query: 181 KGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSV 240
           K I+ NFGTFESAVLGVANA+Q  + RRSRP +  P I     PK P +E+KKE G+W+V
Sbjct: 181 KKIVGNFGTFESAVLGVANADQLLKLRRSRPRKPRPQIPGPPPPKGPTIEHKKEIGLWAV 240

Query: 241 RIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLG 300
           R+PSADS+V+RRTL TLAENPQGLPG NE+ D+IEQRK FWSSVKPA FGVKI  KS +G
Sbjct: 241 RLPSADSTVVRRTLSTLAENPQGLPGVNESADEIEQRKTFWSSVKPAHFGVKIGTKSLIG 300

Query: 301 ILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVG 360
           ILR I VG+FIG+L K +FGRWLLLTFPS+FSLG     + KGPSE+EVN+ SFKMWF+G
Sbjct: 301 ILRIITVGMFIGLLGKTSFGRWLLLTFPSVFSLGWF---RKKGPSEEEVNSASFKMWFIG 360

Query: 361 HGFDDNGN------NKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGI 414
           HGF  + N        EIVTR+MGPE GY+TT IIL+QCA I+L+KR+ +PKGGVLTPGI
Sbjct: 361 HGFRSSNNEGNVEPEMEIVTRVMGPEIGYLTTPIILVQCALIVLSKRESLPKGGVLTPGI 420

[Close]
BLAST of PI0028460 vs. NCBI nr
Match: KAE8651206.1 (hypothetical protein Csa_000763 [Cucumis sativus])

HSP 1 Score: 703.7 bits (1815), Expect = 9.3e-199
Identity = 356/413 (86.20%), Postives = 369/413 (89.35%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFLGSSPLKSLALAGRNLTKLTESLKWAAHPLPP 60
           MEEP Y+L+ILGATGFTGKYVVREALKFL  SPLKSLALAGRNLTKL ESLKWAAHPLPP
Sbjct: 1   MEEPPYDLVILGATGFTGKYVVREALKFLNISPLKSLALAGRNLTKLNESLKWAAHPLPP 60

Query: 61  PPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPEF 120
           PPIPLLLADVTDPHSIRRLCSYTKLILNCVGPF+RYGK VV ACVETGCDYLDICGEPEF
Sbjct: 61  PPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFRRYGKAVVEACVETGCDYLDICGEPEF 120

Query: 121 MERMEAEYDKMAVEKGSLVVSGCGWVEAYMNVESKKGIICNFGTFESAVLGVANAEQFRQ 180
           MERMEAEYDKMAVEKGSLVVSGCG+                    E AVLGVANA + RQ
Sbjct: 121 MERMEAEYDKMAVEKGSLVVSGCGFDSV---------------PAELAVLGVANAGELRQ 180

Query: 181 FRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSVRIPSADSSVIRRTLFTLAENPQGLP 240
           FRRSRP RRSPLI+  STPK PLLEYKK+FGVWSVRIPSADSSVIRRTLFTLAENPQGL 
Sbjct: 181 FRRSRPPRRSPLIVGRSTPKGPLLEYKKQFGVWSVRIPSADSSVIRRTLFTLAENPQGLL 240

Query: 241 GSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLGILRFIVVGIFIGMLAKRAFGRWLLL 300
           GSNET DDIEQRKKFWSSVKPARFGVKI MKSFLGILRFIV GIFIGMLAK+AFGRWLLL
Sbjct: 241 GSNETLDDIEQRKKFWSSVKPARFGVKIGMKSFLGILRFIVFGIFIGMLAKKAFGRWLLL 300

Query: 301 TFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVGHGFDDNGNNKEIVTRIMGPEPGYIT 360
           TFPS+FSLGLL     K PSEDEVNN SFKMWFVGHGFDDNGN+KEIVTRIMGPEPGYIT
Sbjct: 301 TFPSIFSLGLL---NKKSPSEDEVNNVSFKMWFVGHGFDDNGNDKEIVTRIMGPEPGYIT 360

Query: 361 TSIILIQCAFILLTKRQVMPKGGVLTPGIVFGPTHLQQRLQDNGVSFDVISNN 414
           TSIILIQCAFILLTKRQV+PKGGVLTPGIVFGPTHLQQ+LQDNG+SFDVISNN
Sbjct: 361 TSIILIQCAFILLTKRQVLPKGGVLTPGIVFGPTHLQQKLQDNGISFDVISNN 395

[Close]
BLAST of PI0028460 vs. NCBI nr
Match: XP_038905773.1 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Benincasa hispida])

HSP 1 Score: 696.4 bits (1796), Expect = 1.5e-196
Identity = 348/444 (78.38%), Postives = 372/444 (83.78%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFLGSSPLKSLALAGRNLTKLTESLKWAAHPLPP 60
           MEEP Y+LIILGATGFTGKYVVREALKFL SSP KSLALAGRN+TKLTESLKWAAHPLPP
Sbjct: 1   MEEPPYDLIILGATGFTGKYVVREALKFLNSSPFKSLALAGRNMTKLTESLKWAAHPLPP 60

Query: 61  PPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPEF 120
           PPIPLL+ADVTD  SI RLC+ TKLILNC+GPF+RYG+ VVAACVETGCDYLDICGEPEF
Sbjct: 61  PPIPLLIADVTDSQSIHRLCTQTKLILNCIGPFRRYGEPVVAACVETGCDYLDICGEPEF 120

Query: 121 MERMEAEYDKMAVEKGSLVVSGCG------------------------WVEAYMNVESKK 180
           MERMEAEYDK AVE GSLVVSGCG                        WVEAYMNVESKK
Sbjct: 121 MERMEAEYDKRAVESGSLVVSGCGFDSVPAELGLIYNSRQWVGKSAPSWVEAYMNVESKK 180

Query: 181 GIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSVR 240
           G++CNFGTFESAVLGVANA+Q RQFRRSRP RR PLI  CS PK PLLEYKK+ G+W V+
Sbjct: 181 GMVCNFGTFESAVLGVANADQLRQFRRSRPPRRRPLIPYCSAPKEPLLEYKKDVGLWCVK 240

Query: 241 IPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLGI 300
           IPSADSSV+ RTL TLAENPQGLPG NE+P DIEQRK FWSSVKPARF VKI  KSFLGI
Sbjct: 241 IPSADSSVVHRTLSTLAENPQGLPGQNESPQDIEQRKNFWSSVKPARFAVKIGKKSFLGI 300

Query: 301 LRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVGH 360
           LRFI+VGIFIGM AK+AFGRWLLLTFPS+FSLGLLC SKNKGPSED++NN SFKMWFVG 
Sbjct: 301 LRFIMVGIFIGMFAKKAFGRWLLLTFPSIFSLGLLC-SKNKGPSEDDINNSSFKMWFVGR 360

Query: 361 GFDDN-------GNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGI 414
           GFD N       G NKEIVTR+MGPEPGYITTSIILIQCA I+L KR V+PKGGVLTP I
Sbjct: 361 GFDSNYGENYQEGYNKEIVTRVMGPEPGYITTSIILIQCALIVLNKRGVLPKGGVLTPAI 420

[Close]
BLAST of PI0028460 vs. NCBI nr
Match: XP_008443614.1 (PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Cucumis melo] >KAA0053797.1 putative mitochondrial saccharopine dehydrogenase-like oxidoreductase [Cucumis melo var. makuwa] >TYK25604.1 putative mitochondrial saccharopine dehydrogenase-like oxidoreductase [Cucumis melo var. makuwa])

HSP 1 Score: 620.9 bits (1600), Expect = 7.9e-174
Identity = 317/447 (70.92%), Postives = 351/447 (78.52%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFL---GSSPLKSLALAGRNLTKLTESLKWAAHP 60
           +  P Y+LIILGATGFTGKY+VREALKFL    SSPLKSLALAGR+  KLT +LKWAAHP
Sbjct: 10  LSTPPYDLIILGATGFTGKYIVREALKFLNPSSSSPLKSLALAGRDHAKLTNALKWAAHP 69

Query: 61  LPPPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGE 120
           LP PPIPLL++D+T P SIR LC+ TKLILNCVGPF+RYG+ VVAACVETGCDYLDICGE
Sbjct: 70  LPSPPIPLLISDITKPQSIRHLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGE 129

Query: 121 PEFMERMEAEYDKMAVEKGSLVVSGCG------------------------WVEAYMNVE 180
           PEFMERMEA+YD  AVE GSLVVSGCG                        WVEAY+NVE
Sbjct: 130 PEFMERMEAKYDGRAVETGSLVVSGCGFDSIPAELGLIFNLRHWVGKSTPSWVEAYVNVE 189

Query: 181 SKKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVW 240
             +G+  NFGT+ESAVLGVANA+   Q RRSRP RR P     + PK PL+E+KKE G+W
Sbjct: 190 CSEGMTYNFGTYESAVLGVANADALVQLRRSRPPRRRPKFTGGAPPKGPLIEHKKEIGLW 249

Query: 241 SVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSF 300
           +VRIPSADSSV+RRTL TLA+NPQ           I+    FWSSVKPARF VKI  KSF
Sbjct: 250 AVRIPSADSSVVRRTLSTLAQNPQ----------QIKHMNTFWSSVKPARFIVKIGTKSF 309

Query: 301 LGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWF 360
           L ILR +  G+FIGM AK  FGRWLLLTFPS+F+LGL      KGPSEDEVNNGSFKMWF
Sbjct: 310 LEILRCVATGMFIGMFAKNPFGRWLLLTFPSIFTLGLF---NKKGPSEDEVNNGSFKMWF 369

Query: 361 VGHGFDDNGNNKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGIVFGP 420
           VGHGFDDNGN+KEIVTRIMGPEPGYITTSIILIQCAF+LL KR VMPKGGVLTPGIVFGP
Sbjct: 370 VGHGFDDNGNDKEIVTRIMGPEPGYITTSIILIQCAFVLLAKRHVMPKGGVLTPGIVFGP 429

[Close]
BLAST of PI0028460 vs. NCBI nr
Match: XP_008443363.1 (PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Cucumis melo] >KAA0053796.1 putative mitochondrial saccharopine dehydrogenase-like oxidoreductase [Cucumis melo var. makuwa] >TYK25605.1 putative mitochondrial saccharopine dehydrogenase-like oxidoreductase [Cucumis melo var. makuwa])

HSP 1 Score: 594.7 bits (1532), Expect = 6.1e-166
Identity = 298/444 (67.12%), Postives = 349/444 (78.60%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFLG-SSPLKSLALAGRNLTKLTESLKWAAHPLP 60
           ME P Y+LIILGATGFTGKYVVREALKFL  SSPLKS ALAGRNLTKLT++L+WAAHP  
Sbjct: 1   MEGPPYDLIILGATGFTGKYVVREALKFLNPSSPLKSFALAGRNLTKLTQTLQWAAHPHS 60

Query: 61  PPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPE 120
           PPPIP L+AD+ DP SI RLC+ TKLILNCVGPF+RYG+ VVAACVETGCDYLDICGEPE
Sbjct: 61  PPPIPFLIADIADPQSIHRLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGEPE 120

Query: 121 FMERMEAEYDKMAVEKGSLVVSGCGW------------------------VEAYMNVESK 180
           FME+MEA Y + AV+ G+LVVS CG+                        +EAY+++ESK
Sbjct: 121 FMEKMEANYHERAVQSGALVVSACGFDSVPAELGLMFNSRQWVGQMAPNRIEAYLSLESK 180

Query: 181 KGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSV 240
           K I+ NFGTFESAVLGVANA+Q  + RRSRP +  P I     PK P +E+KKE G+W+V
Sbjct: 181 KKIVGNFGTFESAVLGVANADQLLKLRRSRPRKPRPQIPGPPPPKGPTIEHKKEIGLWAV 240

Query: 241 RIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLG 300
           R+PSADS+V+RRTL TLAENPQGLPG NE+ D+IEQRK FWSSVKPA FGVKI  KS +G
Sbjct: 241 RLPSADSTVVRRTLSTLAENPQGLPGVNESADEIEQRKTFWSSVKPAHFGVKIGTKSLIG 300

Query: 301 ILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVG 360
           ILR I VG+FIG+L K +FGRWLLLTFPS+FSLG     + KGPSE+EVN+ SFKMWF+G
Sbjct: 301 ILRIITVGMFIGLLGKTSFGRWLLLTFPSVFSLGWF---RKKGPSEEEVNSASFKMWFIG 360

Query: 361 HGFDDNGN------NKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGI 414
           HGF  + N        EIVTR+MGPE GY+TT IIL+QCA I+L+KR+ +PKGGVLTPGI
Sbjct: 361 HGFRSSNNEGNVEPEMEIVTRVMGPEIGYLTTPIILVQCALIVLSKRESLPKGGVLTPGI 420

[Close]
BLAST of PI0028460 vs. NCBI nr
Match: XP_038903070.1 (probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Benincasa hispida])

HSP 1 Score: 586.3 bits (1510), Expect = 2.2e-163
Identity = 297/444 (66.89%), Postives = 347/444 (78.15%), Query Frame = 0 

Query: 1   MEEPSYNLIILGATGFTGKYVVREALKFLGSS-PLKSLALAGRNLTKLTESLKWAAHPLP 60
           MEEP Y+LIILGATGFTGKYVVREALKFL SS PLKS ALAGRNLTKLTE+LKWAAHP  
Sbjct: 1   MEEPPYDLIILGATGFTGKYVVREALKFLNSSFPLKSFALAGRNLTKLTETLKWAAHPHS 60

Query: 61  PPPIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEPE 120
           PPPIPLL+AD+ DP SIRRLC+ T LILNCVGPF+ YG+ VVAACVETGCDYLDICGEPE
Sbjct: 61  PPPIPLLIADIADPQSIRRLCTQTNLILNCVGPFRYYGEPVVAACVETGCDYLDICGEPE 120

Query: 121 FMERMEAEYDKMAVEKGSLVVSGCGW------------------------VEAYMNVESK 180
           FME+MEA Y + AV+ G+LVVS CG+                        +EAYM++ESK
Sbjct: 121 FMEKMEANYHERAVQSGALVVSACGFDSVPAELGFIFNSRQWVGQSAPNRIEAYMSLESK 180

Query: 181 KGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTPKRPLLEYKKEFGVWSV 240
           + ++ NFGTFESAVLGVANA+Q  + RRSRP R  P I     PK P +E+KK+ G+W+V
Sbjct: 181 RRVVWNFGTFESAVLGVANADQLLKLRRSRPRRPRPKIPGPPPPKGPTIEHKKDIGLWAV 240

Query: 241 RIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSFLG 300
           R+PSADSSV+RRTL TLAENPQGLPG NE+ D+IEQRK FWSSVKPA FGVK+  KS +G
Sbjct: 241 RLPSADSSVVRRTLSTLAENPQGLPGVNESADEIEQRKTFWSSVKPAHFGVKLGSKSLIG 300

Query: 301 ILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWFVG 360
           ILR I VG+ IG+LA+ + GRWLLLTFP +FSLG     + KGPSED VN+ SFKMWFVG
Sbjct: 301 ILRIITVGMLIGLLARTSLGRWLLLTFPLVFSLGWF---RRKGPSEDGVNSASFKMWFVG 360

Query: 361 HGF---DDNGN---NKEIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVLTPGI 414
           HGF   +  GN   + EIVTR+MGPE GY+TT+IIL+QCA I+L+KR+ +P+GG LTPGI
Sbjct: 361 HGFSSSNSEGNAEPDMEIVTRVMGPEIGYLTTAIILVQCALIVLSKREALPQGGTLTPGI 420

[Close]
BLAST of PI0028460 vs. TAIR 10
Match: AT5G39410.1 (Saccharopine dehydrogenase )

HSP 1 Score: 498.8 bits (1283), Expect = 4.2e-141
Identity = 256/446 (57.40%), Postives = 325/446 (72.87%), Query Frame = 0 

Query: 3   EPSYNLIILGATGFTGKYVVREALKFL---GSSPLKSLALAGRNLTKLTESLKWAAHPLP 62
           EP Y+++ILGA+GFTGKYVVREALKFL    SSPLKSLALAGRN T+LT+SL+WAA P P
Sbjct: 8   EPVYDMVILGASGFTGKYVVREALKFLQTPSSSPLKSLALAGRNPTRLTQSLEWAARPNP 67

Query: 63  PP-PIPLLLADVTDPHSIRRLCSYTKLILNCVGPFQRYGKVVVAACVETGCDYLDICGEP 122
           PP  + +L AD +DP S+RRLC+ TKLILNCVGPF+ +G  VV+AC ++GCDYLDI GEP
Sbjct: 68  PPSSVAILTADTSDPDSLRRLCTQTKLILNCVGPFRIHGDPVVSACADSGCDYLDISGEP 127

Query: 123 EFMERMEAEYDKMAVEKGSLVVSGCGW------------------------VEAYMNVES 182
           EFMERMEA Y   A E GSL+VS CG+                        +EAY+++ES
Sbjct: 128 EFMERMEANYHDRAEETGSLIVSACGFDSIPAELGLLFNAKQWVSPSVPNQIEAYLSLES 187

Query: 183 KKGIICNFGTFESAVLGVANAEQFRQFRRSRPLRRSPLILSCSTP-KRPLLEYKKEFGVW 242
            K I  NFGT+ESAVLGVANAE+ ++ RRSRP R  P I  C  P K P LE +K  G+W
Sbjct: 188 DKKIAGNFGTYESAVLGVANAEKLKELRRSRPRRPRPTI--CGPPAKGPTLENQKTIGLW 247

Query: 243 SVRIPSADSSVIRRTLFTLAENPQGLPGSNETPDDIEQRKKFWSSVKPARFGVKISMKSF 302
           ++++PSAD+ V+RRTL TL E P GLPG NE+P+ I++R+ FWSS+KPA FGVKI+ KS 
Sbjct: 248 ALKLPSADAVVVRRTLTTLTEKPHGLPGINESPEQIQKREAFWSSIKPAHFGVKITSKSL 307

Query: 303 LGILRFIVVGIFIGMLAKRAFGRWLLLTFPSLFSLGLLCSSKNKGPSEDEVNNGSFKMWF 362
            GI R++ +G+ +G+L+K +FGRWLLL FPS+FSLG     + KGPSE+EV + +FKMWF
Sbjct: 308 FGIFRYVTLGVSLGLLSKFSFGRWLLLKFPSVFSLGWF---QKKGPSEEEVESATFKMWF 367

Query: 363 VGHGFDD-----NGNNK---EIVTRIMGPEPGYITTSIILIQCAFILLTKRQVMPKGGVL 412
           +G G+ +      G  K   EI+TRI GPE GYITT I L+QC  I+L +R+ + KGGV 
Sbjct: 368 IGRGYSEESLASQGETKPDLEIITRISGPEIGYITTPITLVQCGLIVLGQRESLVKGGVY 427

The following BLAST results are available for this feature:
BLAST of PI0028460 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LGI25.9e-14057.40Probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
Q8R1271.4e-5630.51Saccharopine dehydrogenase-like oxidoreductase OS=Mus musculus OX=10090 GN=Sccpd... [more]
Q3T0673.4e-5530.80Saccharopine dehydrogenase-like oxidoreductase OS=Bos taurus OX=9913 GN=SCCPDH P... [more]
Q6AY304.5e-5530.29Saccharopine dehydrogenase-like oxidoreductase OS=Rattus norvegicus OX=10116 GN=... [more]
Q8NBX01.1e-5329.62Saccharopine dehydrogenase-like oxidoreductase OS=Homo sapiens OX=9606 GN=SCCPDH... [more]
BLAST of PI0028460 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LCQ27.4e-21089.35Sacchrp_dh_NADP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G82... [more]
A0A5D3DPV43.8e-17470.92Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Cucumis... [more]
A0A1S3B7Z43.8e-17470.92probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
A0A5D3DQ442.9e-16667.12Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Cucumis... [more]
A0A1S3B8K42.9e-16667.12probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
BLAST of PI0028460 vs. NCBI nr
Match NameE-valueIdentityDescription
KAE8651206.19.3e-19986.20hypothetical protein Csa_000763 [Cucumis sativus][more]
XP_038905773.11.5e-19678.38probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
XP_008443614.17.9e-17470.92PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase... [more]
XP_008443363.16.1e-16667.12PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase... [more]
XP_038903070.12.2e-16366.89probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
BLAST of PI0028460 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G39410.14.2e-14157.40Saccharopine dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005097Saccharopine dehydrogenase, NADP binding domainPFAMPF03435Sacchrp_dh_NADPcoord: 9..140
e-value: 2.2E-11
score: 44.1
NoneNo IPR availableGENE3D3.40.50.720coord: 7..181
e-value: 2.1E-23
score: 85.0
NoneNo IPR availablePANTHERPTHR12286UNCHARACTERIZEDcoord: 146..413
NoneNo IPR availablePANTHERPTHR12286:SF9MITOCHONDRIAL SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE-RELATEDcoord: 3..144
coord: 146..413
NoneNo IPR availablePANTHERPTHR12286UNCHARACTERIZEDcoord: 3..144
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 6..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028460.1PI0028460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009247 glycolipid biosynthetic process
cellular_component GO:0005811 lipid droplet
cellular_component GO:0016020 membrane
molecular_function GO:0016491 oxidoreductase activity