PI0028436 (gene) Melon (PI 482460) v1

Overview


Name	PI0028436
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	(R)-specific enoyl-CoA hydratase
Location	chr11: 5958982 .. 5959753 (+)
RNA-Seq Expression	PI0028436
Synteny	PI0028436

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAAGTAGAGGTTTATTCTCAACATATATTTCTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCTATAACAGAATATTCACCAGTGAAGATGTTTGGAATACTCCAAAGTGAGTCATGACTCTAATCCTCTGCATTTCGATCCCGAATTAGCTCGACGTGCTGGATTCAACAGTTGTCTTGTTCACGGTTTGCTTGTTGCTTCGATGTTCCCTCATATCATTTCCTCTCATTATGTAAGTCTAGCCCCTTTGCATTTCTTACACTTTGTAGAACTTTGGTGGGACTGAGAAATGGAACCTCAAACGTTCTTACAACTGATTTAATGTTTATCTTTTAATGTATGGTGATGGTCTAATCTTTGAAGATGCTCTGATTTATAATGTTGAGGATGATTGTAGCCCGGAGCCATCTACGTGTCGCAAAGTTTAAATTTTAAGTTGCCGGTTTATGTTGGAGAGAAGATTGTTGGTGTGGTAGAAGCAATCGACTTGAGAGAAAATAAGAAGAGATACCTGTAAGCTTGTTCTTGGGCAAATCTCTACCTTTTCAAATTCAAATTTCATGTTATCATTTCATTGAGTTGGTTACTTGCCAAATGTGTTTCAGGGTGAAATTCAAAACAAAGTGCTTGAGGAATGGTGACGAGCTTGTTCTCGAGGGCGAGGCAAGGGCCATCTTACCAACTGATTTTCATTCCATAAACAGAGTAGAAGAAGATGTTCAAACTTCAAAGATTTAA

mRNA sequence

ATGCTAAGTAGAGGTTTATTCTCAACATATATTTCTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGATGAGTCATGACTCTAATCCTCTGCATTTCGATCCCGAATTAGCTCGACGTGCTGGATTCAACAGTTGTCTTGTTCACGGTTTGCTTGTTGCTTCGATGTTCCCTCATATCATTTCCTCTCATTATCCCGGAGCCATCTACGTGTCGCAAAGTTTAAATTTTAAGTTGCCGGTTTATGTTGGAGAGAAGATTGTTGGTGTGGTAGAAGCAATCGACTTGAGAGAAAATAAGAAGAGATACCTGGTGAAATTCAAAACAAAGTGCTTGAGGAATGGTGACGAGCTTGTTCTCGAGGGCGAGGCAAGGGCCATCTTACCAACTGATTTTCATTCCATAAACAGAGTAGAAGAAGATGTTCAAACTTCAAAGATTTAA

Coding sequence (CDS)

ATGCTAAGTAGAGGTTTATTCTCAACATATATTTCTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGATGAGTCATGACTCTAATCCTCTGCATTTCGATCCCGAATTAGCTCGACGTGCTGGATTCAACAGTTGTCTTGTTCACGGTTTGCTTGTTGCTTCGATGTTCCCTCATATCATTTCCTCTCATTATCCCGGAGCCATCTACGTGTCGCAAAGTTTAAATTTTAAGTTGCCGGTTTATGTTGGAGAGAAGATTGTTGGTGTGGTAGAAGCAATCGACTTGAGAGAAAATAAGAAGAGATACCTGGTGAAATTCAAAACAAAGTGCTTGAGGAATGGTGACGAGCTTGTTCTCGAGGGCGAGGCAAGGGCCATCTTACCAACTGATTTTCATTCCATAAACAGAGTAGAAGAAGATGTTCAAACTTCAAAGATTTAA

Protein sequence

MLSRGLFSTYISSLKCFSSSTSNVLKVGDILMSHDSNPLHFDPELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAIDLRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSINRVEEDVQTSKI

Homology

[Close]

BLAST of PI0028436 vs. ExPASy Swiss-Prot
Match: O32472 ((R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.6e-16
Identity = 45/108 (41.67%), Postives = 63/108 (58.33%), Query Frame = 0


Query: 32  MSHDSNPLHFDPELARRAGFNSCLVHGLLVASMFPHIISSHYP--GAIYVSQSLNFKLPV 91
           +S D NPLH DP  A    F   +VHG+L+AS+F  ++    P  G+IY+ QSL+FKLPV
Sbjct: 29  LSEDFNPLHLDPAFAATTAFERPIVHGMLLASLFSGLLGQQLPGKGSIYLGQSLSFKLPV 88

Query: 92  YVGEKIVGVVEAIDLRENKKRYLVKFKTKCLRNGDELVLEGEARAILP 138
           +VG+++   VE   LRE+K   +    T+    G  L + GEA   LP
Sbjct: 89  FVGDEVTAEVEVTALREDKP--IATLTTRIFTQGGALAVTGEAVVKLP 134

[Close]

BLAST of PI0028436 vs. ExPASy Swiss-Prot
Match: Q2RQ36 ((R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=phaJ PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.3e-12
Identity = 44/126 (34.92%), Postives = 68/126 (53.97%), Query Frame = 0


Query: 18  SSSTSNVLKVGDIL----MSHDSNPLHFDPELARRAGFNSCLVHGLLVASMFPHIISSHY 77
           S+S +  +   DI     +S D+NP H + + A+   F + + HG+L A     ++ +  
Sbjct: 19  SASLAKTISESDIYLFAGLSMDTNPAHVNEDYAQTTVFKTRIAHGMLSAGFISAVLGTRL 78

Query: 78  --PGAIYVSQSLNFKLPVYVGEKIVGVVEAIDLRENKKRYLVKFKTKCLRNGDELVLEGE 137
             PGAIYV+QSL FK PV +G+ +   V    L   KK   V F+T C   G ++V+EGE
Sbjct: 79  PGPGAIYVNQSLKFKAPVRIGDTVTATVTVTGLVPEKK--FVTFRTTCTVAG-KVVIEGE 138

[Close]

BLAST of PI0028436 vs. ExPASy Swiss-Prot
Match: C5AZ74 (3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) OX=272630 GN=croR PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-11
Identity = 42/120 (35.00%), Postives = 67/120 (55.83%), Query Frame = 0


Query: 20  STSNVLKVGDILMSHDSNPLHFDPELARRAGFNSCLVHGLLVASMFPHIISSHY--PGAI 79
           S+S+V  VG   ++ D NP+H     A R  F + + HGL  A +   ++ +    PGA+
Sbjct: 26  SSSDV--VGFAEITGDRNPIHLSEHFAARTPFGTRIAHGLYTAGLISAVLGTRLPGPGAV 85

Query: 80  YVSQSLNFKLPVYVGEKIVGVVEAIDLRENKKRYLVKFKTKCLRNGDELVLEGEARAILP 138
           Y+SQ+LNF+ PV +G+ +   VE  +L   ++R   +    C   GDE+VL+GEA   +P
Sbjct: 86  YISQTLNFRAPVRIGDIVEVKVEVAELIPERRR--ARLACTC-SVGDEVVLDGEALVKVP 140

[Close]

BLAST of PI0028436 vs. ExPASy Swiss-Prot
Match: P86397 (Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 GN=HTD2 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.5e-08
Identity = 29/109 (26.61%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 32  MSHDSNPLHFDPELARRAGFNSCLVHGLLVASMFPHIISSHY--PGAIYVSQSLNFKLPV 91
           ++ D NPLH + + A+   F + +VHG+L+  +   ++ +    PG +++SQ ++F  P+
Sbjct: 59  LTGDVNPLHLNEDFAKHTKFGNTIVHGVLINGLISALLGTKMPGPGCVFLSQEISFPAPL 118

Query: 92  YVGEKIVGVVEAIDLRENKKRYLVKFKTKC-LRNGDELVLEGEARAILP 138
           Y+GE ++   E   L    KR++      C +    + V+EG  + ++P
Sbjct: 119 YIGEVVLASAEVKKL----KRFIAIIAVSCSVIESKKTVMEGWVKVMVP 163

[Close]

BLAST of PI0028436 vs. ExPASy Swiss-Prot
Match: Q9VXJ0 (Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=Mfe2 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.0e-04
Identity = 27/99 (27.27%), Postives = 49/99 (49.49%), Query Frame = 0


Query: 32  MSHDSNPLHFDPELARRAGFNSCLVHGLLVASMFPHIISSHYPG---AIYVSQSLNFKLP 91
           +S D NPLH DP++A  AGF + ++HGL         + + +     A++ +  + F  P
Sbjct: 493 LSGDKNPLHIDPQMALLAGFKTPILHGLCTLGFSVRAVLAQFADNNPALFKAVKVRFSGP 552

Query: 92  VYVGEKIVGVVEAIDLRENKKRYLVKFKTKCLRNGDELV 128
           V  G+ +      +DL +   R  + F+T  +  G E++
Sbjct: 553 VIPGQTL-----RVDLWKQGTR--INFRTVVVETGKEVI 584

[Close]

BLAST of PI0028436 vs. ExPASy TrEMBL
Match: A0A5A7T147 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00540 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.3e-65
Identity = 135/161 (83.85%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 1   MLSRGLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFDP 60
           MLSRGLFST+I SSLKCFSSSTSNVLKVGDIL                +SHDSNPLHFDP
Sbjct: 1   MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 60

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           ELARRAGFN CLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVG VEAI+
Sbjct: 61  ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSIN 145
           LRENKKRYL KFKTKCLRNGDELVLEGEARAILP  FHS N
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]

BLAST of PI0028436 vs. ExPASy TrEMBL
Match: A0A1S3CLU3 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.3e-65
Identity = 135/161 (83.85%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 1   MLSRGLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFDP 60
           MLSRGLFST+I SSLKCFSSSTSNVLKVGDIL                +SHDSNPLHFDP
Sbjct: 45  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 104

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           ELARRAGFN CLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVG VEAI+
Sbjct: 105 ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 164

Query: 121 LRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSIN 145
           LRENKKRYL KFKTKCLRNGDELVLEGEARAILP  FHS N
Sbjct: 165 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of PI0028436 vs. ExPASy TrEMBL
Match: A0A0A0KCN3 (MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 7.7e-64
Identity = 132/161 (81.99%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 1   MLSRGLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFDP 60
           MLS+GLFSTYI SSLKCFSSSTS+VLKVGDIL                +SHDSNPLHFD 
Sbjct: 1   MLSKGLFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDS 60

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           +LA+RAGFN CLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVG VEAI+
Sbjct: 61  KLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSIN 145
           LRENKKRYL KFKTKCLRNGDELVLEGEARAILP DFHS N
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of PI0028436 vs. ExPASy TrEMBL
Match: A0A6J1JDU3 (uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.6e-53
Identity = 120/160 (75.00%), Postives = 130/160 (81.25%), Query Frame = 0


Query: 1   MLSR-GLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFD 60
           MLSR GL S+YI SSLKCFSSSTSNVLKVGDIL                +SHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  PELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAI 120
             LARRAGF+  LVHG+LVASMFP +ISSH+PGAIYVSQSLNFKLPVYVGE I+  VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHS 143
           DLRE KKRYL KF+TKCLRNGDELVL+GEARAILP+DF S
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCS 160

[Close]

BLAST of PI0028436 vs. ExPASy TrEMBL
Match: A0A6J1CFA0 (uncharacterized protein LOC111011158 OS=Momordica charantia OX=3673 GN=LOC111011158 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-52
Identity = 114/163 (69.94%), Postives = 129/163 (79.14%), Query Frame = 0


Query: 1   MLSRGLFSTYI--SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFD 60
           ML RG+FS +I  SSLK FSSSTSNVLKVGDIL                +SHDSNPLHFD
Sbjct: 1   MLRRGVFSAFIVSSSLKFFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  PELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAI 120
             LARRAG+   LVHG+LVASMFP IISSH+PGAIYVSQSLNFKLPVYVGE+I+G VEA+
Sbjct: 61  SALARRAGYEDRLVHGMLVASMFPLIISSHFPGAIYVSQSLNFKLPVYVGEQIIGQVEAV 120

Query: 121 DLRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSINR 146
           +L+ENKKRYL KF+T CLRNG +LVLEGEA AILP+DFH  +R
Sbjct: 121 NLKENKKRYLAKFRTLCLRNGGDLVLEGEAMAILPSDFHPTDR 163

[Close]

BLAST of PI0028436 vs. NCBI nr
Match: XP_008463911.1 (PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo])

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-64
Identity = 135/161 (83.85%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 1   MLSRGLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFDP 60
           MLSRGLFST+I SSLKCFSSSTSNVLKVGDIL                +SHDSNPLHFDP
Sbjct: 45  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 104

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           ELARRAGFN CLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVG VEAI+
Sbjct: 105 ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 164

Query: 121 LRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSIN 145
           LRENKKRYL KFKTKCLRNGDELVLEGEARAILP  FHS N
Sbjct: 165 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of PI0028436 vs. NCBI nr
Match: KAA0035311.1 ((R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa])

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-64
Identity = 135/161 (83.85%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 1   MLSRGLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFDP 60
           MLSRGLFST+I SSLKCFSSSTSNVLKVGDIL                +SHDSNPLHFDP
Sbjct: 1   MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 60

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           ELARRAGFN CLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVG VEAI+
Sbjct: 61  ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSIN 145
           LRENKKRYL KFKTKCLRNGDELVLEGEARAILP  FHS N
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]

BLAST of PI0028436 vs. NCBI nr
Match: XP_004149553.1 (uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical protein Csa_022963 [Cucumis sativus])

HSP 1 Score: 253.1 bits (645), Expect = 1.6e-63
Identity = 132/161 (81.99%), Postives = 138/161 (85.71%), Query Frame = 0


Query: 1   MLSRGLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFDP 60
           MLS+GLFSTYI SSLKCFSSSTS+VLKVGDIL                +SHDSNPLHFD 
Sbjct: 1   MLSKGLFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDS 60

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           +LA+RAGFN CLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVG VEAI+
Sbjct: 61  KLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSIN 145
           LRENKKRYL KFKTKCLRNGDELVLEGEARAILP DFHS N
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of PI0028436 vs. NCBI nr
Match: XP_038901209.1 ((R)-specific enoyl-CoA hydratase [Benincasa hispida])

HSP 1 Score: 239.6 bits (610), Expect = 1.8e-59
Identity = 126/161 (78.26%), Postives = 134/161 (83.23%), Query Frame = 0


Query: 1   MLSRGLFSTYIS-SLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFDP 60
           MLSRGLFS +IS SLKCFSSSTSNVLKVGDIL                +SHDSNPLHFD 
Sbjct: 1   MLSRGLFSMHISTSLKCFSSSTSNVLKVGDILSYTRIFTSKDVLEYSKVSHDSNPLHFDS 60

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           ELARRAGFN  LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGE IVG VEAID
Sbjct: 61  ELARRAGFNGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGENIVGQVEAID 120

Query: 121 LRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHSIN 145
           LRENKKRYL KF TKC+RNG E+VLEGEARAI+P+DFHS N
Sbjct: 121 LRENKKRYLAKFITKCMRNGHEVVLEGEARAIIPSDFHSTN 161

[Close]

BLAST of PI0028436 vs. NCBI nr
Match: XP_022985629.1 (uncharacterized protein LOC111483634 [Cucurbita maxima])

HSP 1 Score: 218.8 bits (556), Expect = 3.3e-53
Identity = 120/160 (75.00%), Postives = 130/160 (81.25%), Query Frame = 0


Query: 1   MLSR-GLFSTYI-SSLKCFSSSTSNVLKVGDIL----------------MSHDSNPLHFD 60
           MLSR GL S+YI SSLKCFSSSTSNVLKVGDIL                +SHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  PELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAI 120
             LARRAGF+  LVHG+LVASMFP +ISSH+PGAIYVSQSLNFKLPVYVGE I+  VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLRENKKRYLVKFKTKCLRNGDELVLEGEARAILPTDFHS 143
           DLRE KKRYL KF+TKCLRNGDELVL+GEARAILP+DF S
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCS 160

[Close]

BLAST of PI0028436 vs. TAIR 10
Match: AT5G60335.1 (Thioesterase superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 2.6e-40
Identity = 84/155 (54.19%), Postives = 115/155 (74.19%), Query Frame = 0


Query: 1   MLSRGLFSTYISSLKCFSS-STSNVLKVGDIL----------------MSHDSNPLHFDP 60
           ML++ +F   +  L+ FSS ++  +LKVGD+L                +SHD NPLHFDP
Sbjct: 1   MLAKTVFPRGLLVLRSFSSVASKTLLKVGDVLRETRVFSSEDIKAYAEVSHDWNPLHFDP 60

Query: 61  ELARRAGFNSCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGVVEAID 120
           E AR+AGF + LVHG+LV+SMFP IIS+H+PGA+YVSQSL+F+ PVY+G++I+G+V+AI 
Sbjct: 61  ESARKAGFENRLVHGMLVSSMFPRIISAHFPGAVYVSQSLHFRSPVYIGDEILGLVQAIA 120

Query: 121 LRENKKRYLVKFKTKCLRNGDEL-VLEGEARAILP 138
           LRE K +Y+VKF TKC +N +EL V++GEA AILP
Sbjct: 121 LRETKNKYIVKFSTKCFKNHNELVVIDGEATAILP 155

The following BLAST results are available for this feature:

BLAST of PI0028436 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O32472	2.6e-16	41.67	(R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1	[more]
Q2RQ36	2.3e-12	34.92	(R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / A...	[more]
C5AZ74	1.1e-11	35.00	3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 ...	[more]
P86397	1.5e-08	26.61	Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 ...	[more]
Q9VXJ0	3.0e-04	27.27	Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=...	[more]

BLAST of PI0028436 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T147	5.3e-65	83.85	(R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CLU3	5.3e-65	83.85	(R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV...	[more]
A0A0A0KCN3	7.7e-64	81.99	MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 P...	[more]
A0A6J1JDU3	1.6e-53	75.00	uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634...	[more]
A0A6J1CFA0	1.4e-52	69.94	uncharacterized protein LOC111011158 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]

BLAST of PI0028436 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008463911.1	1.1e-64	83.85	PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo]	[more]
KAA0035311.1	1.1e-64	83.85	(R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-spec...	[more]
XP_004149553.1	1.6e-63	81.99	uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical ...	[more]
XP_038901209.1	1.8e-59	78.26	(R)-specific enoyl-CoA hydratase [Benincasa hispida]	[more]
XP_022985629.1	3.3e-53	75.00	uncharacterized protein LOC111483634 [Cucurbita maxima]	[more]

BLAST of PI0028436 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60335.1	2.6e-40	54.19	Thioesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 30..141 e-value: 6.2E-28 score: 99.0
None	No IPR available	PANTHER	PTHR43437	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 32..138
None	No IPR available	PANTHER	PTHR43437:SF3	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 32..138
None	No IPR available	CDD	cd03449	R_hydratase	coord: 32..136 e-value: 2.82035E-37 score: 122.268
IPR002539	MaoC-like dehydratase domain	PFAM	PF01575	MaoC_dehydratas	coord: 32..110 e-value: 2.9E-14 score: 52.7
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 32..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028436.1	PI0028436.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process