PI0028394 (gene) Melon (PI 482460) v1

Overview


Name	PI0028394
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	TIR domain-containing protein
Location	chr12: 4808786 .. 4809500 (-)
RNA-Seq Expression	PI0028394
Synteny	PI0028394

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACGTTATTTTTCAACTCTGGCCAAAAACTACCTAATGTCATCCAAAATCCTCAGTCGTCGTAACGATATCCGATCCATATCACGCCAATGTGACGTCTTTATAAACCACCGTGGCGTCGACACGAAACGTAACATCGCAGGATTGCTTTATGACCATTTTTCTAGAATAGGTCTCCACCCATTTTTAGATAGTAAGAACATGAAACCGGGGGATAAATTGTTTGGTGAGATTGAGGAAGGTATTCGGAGTTGTAAGGTTGGAATTGCGGTGTTCTCACCTCGGTATTGTGAATCCTATTTTTGTTTGCACGAACTTGCCCTAATGGTGGAGAACAAAAGAAAATCATTCCAATTTTCGTTGACGTTCGACCGTCTCAGCTTCGTGTTGAATACAACTATAGTTGTCCCAAGAAAGAGTTGCAAAGGTTCAATTGGGCACTTGGGGAAGCCAAATACACTGTTGGACTCACTTTCGACACTATTAACGGGTAAATTTACTATCTATTTTTTTTCCAATGAAGTGACTGTAGCATAATGCTTTCAATCCTTTTAAATTTTAAAAATATTTATATAAATTGTGGCACTCAAAATCACTCATGTTGTGTTGTTATTTTTGTAGGGATTGGTCGGAGCTGTTGAGGAAAGCTTCAAACGCCGTGATTGATAATCTAATCGTCGGCGGAGGGGCCGGAGAGATGCCGGATAATTAG

mRNA sequence

ATGCAACGTTATTTTTCAACTCTGGCCAAAAACTACCTAATGTCATCCAAAATCCTCAGTCGTCGTAACGATATCCGATCCATATCACGCCAATGTGACGTCTTTATAAACCACCGTGGCGTCGACACGAAACGTAACATCGCAGGATTGCTTTATGACCATTTTTCTAGAATAGGTCTCCACCCATTTTTAGATAGTAAGAACATGAAACCGGGGGATAAATTGTTTGGTGAGATTGAGGAAGGTATTCGGAGTTGTAAGGTTGGAATTGCGGTGTTCTCACCTCGGTATTGTGAATCCTATTTTTGTTTGCACGAACTTGCCCTAATGCTTCGTGTTGAATACAACTATAGTTGTCCCAAGAAAGAGTTGCAAAGGTTCAATTGGGCACTTGGGGAAGCCAAATACACTGTTGGACTCACTTTCGACACTATTAACGGGGATTGGTCGGAGCTGTTGAGGAAAGCTTCAAACGCCGTGATTGATAATCTAATCGTCGGCGGAGGGGCCGGAGAGATGCCGGATAATTAG

Coding sequence (CDS)

ATGCAACGTTATTTTTCAACTCTGGCCAAAAACTACCTAATGTCATCCAAAATCCTCAGTCGTCGTAACGATATCCGATCCATATCACGCCAATGTGACGTCTTTATAAACCACCGTGGCGTCGACACGAAACGTAACATCGCAGGATTGCTTTATGACCATTTTTCTAGAATAGGTCTCCACCCATTTTTAGATAGTAAGAACATGAAACCGGGGGATAAATTGTTTGGTGAGATTGAGGAAGGTATTCGGAGTTGTAAGGTTGGAATTGCGGTGTTCTCACCTCGGTATTGTGAATCCTATTTTTGTTTGCACGAACTTGCCCTAATGCTTCGTGTTGAATACAACTATAGTTGTCCCAAGAAAGAGTTGCAAAGGTTCAATTGGGCACTTGGGGAAGCCAAATACACTGTTGGACTCACTTTCGACACTATTAACGGGGATTGGTCGGAGCTGTTGAGGAAAGCTTCAAACGCCGTGATTGATAATCTAATCGTCGGCGGAGGGGCCGGAGAGATGCCGGATAATTAG

Protein sequence

MQRYFSTLAKNYLMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALMLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVIDNLIVGGGAGEMPDN

Homology

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BLAST of PI0028394 vs. ExPASy Swiss-Prot
Match: I1GTC2 (TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.6e-30
Identity = 67/154 (43.51%), Postives = 99/154 (64.29%), Query Frame = 0


Query: 33  DVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAV 92
           +VFINHRGVDTKR +A LLYD  ++ GL  FLD+ +M+PGD+L   I   IR C V +A+
Sbjct: 54  EVFINHRGVDTKRTVARLLYDRLAQAGLRGFLDNMSMRPGDRLEERIGSAIRECTVAVAI 113

Query: 93  FSPRYCESYFCLHELALMLR-------VEYNYS-----CPK----------KELQRFNWA 152
           FSP YC+S +CL ELA+++        + Y+        P+          ++L+RF +A
Sbjct: 114 FSPSYCDSEYCLRELAMLVESRKAIIPIFYDIKPSDLLLPQAVADSEVYLPRDLERFKFA 173

Query: 153 LGEAKYTVGLTFDTINGDWSELLRKASNAVIDNL 165
           L EAK+TVG+T+D+  GD +EL+  A++AV+ N+
Sbjct: 174 LREAKHTVGITYDSATGDMAELVSAAADAVMYNM 207

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BLAST of PI0028394 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-10
Identity = 29/88 (32.95%), Postives = 47/88 (53.41%), Query Frame = 0


Query: 30  RQCDVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVG 89
           R  DVF++ RG DT++   G L++     G+H F+D K +K G  +  E+ + I   +  
Sbjct: 10  RSYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFA 69

Query: 90  IAVFSPRYCESYFCLHELALMLRVEYNY 118
           + VFS  Y  S +CL EL  +L +   +
Sbjct: 70  VVVFSKNYASSTWCLEELVKILEIHEKF 97

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BLAST of PI0028394 vs. ExPASy Swiss-Prot
Match: F4KIF3 (Disease resistance-like protein CSA1 OS=Arabidopsis thaliana OX=3702 GN=CSA1 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.0e-08
Identity = 32/80 (40.00%), Postives = 48/80 (60.00%), Query Frame = 0


Query: 31  QCDVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGI 90
           Q  VFIN RGV+ ++N    L     R G++ F+D+ + + G +L   + E I   ++ +
Sbjct: 16  QDQVFINFRGVELRKNFVSHLEKGLKRKGINAFIDT-DEEMGQEL-SVLLERIEGSRIAL 75

Query: 91  AVFSPRYCESYFCLHELALM 111
           A+FSPRY ES +CL ELA M
Sbjct: 76  AIFSPRYTESKWCLKELAKM 93

[Close]

BLAST of PI0028394 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.5e-07
Identity = 41/143 (28.67%), Postives = 61/143 (42.66%), Query Frame = 0


Query: 29  SRQCDVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKV 88
           S + DVF++ RG DT+ N  G L       G+  F+D + ++ GD L   + + I   K+
Sbjct: 8   SAEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDDR-LRRGDNLTA-LFDRIEKSKI 67

Query: 89  GIAVFSPRYCESYFCLHELALMLR---------VEYNYSCPKKELQRFNWALGEAKYTVG 148
            I VFS  Y  S +CL EL  +L          V   Y   K ++++   +         
Sbjct: 68  AIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKLPE 127

Query: 149 LTFDTIN----GDWSELLRKASN 159
           LTF  +       W   L  ASN
Sbjct: 128 LTFPGVTPEEISSWKAALASASN 148

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BLAST of PI0028394 vs. ExPASy Swiss-Prot
Match: Q0WSX8 (TIR domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=TIK PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.0e-05
Identity = 27/77 (35.06%), Postives = 40/77 (51.95%), Query Frame = 0


Query: 34  VFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVF 93
           VFIN RG + +      L       G++ F+D+K  K   +    +   I+  K+ +A+F
Sbjct: 13  VFINFRGDELREIFVNHLELQLRNAGINVFIDTKEQK--GRRLQYLFTRIKKSKIALAIF 72

Query: 94  SPRYCESYFCLHELALM 111
           S RYCES +CL EL  M
Sbjct: 73  SKRYCESKWCLDELVTM 87

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BLAST of PI0028394 vs. ExPASy TrEMBL
Match: A0A0A0LV24 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073070 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.5e-90
Identity = 167/194 (86.08%), Postives = 173/194 (89.18%), Query Frame = 0


Query: 1   MQRYFSTLAKNYLMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGL 60
           MQRYFST+AK+YL SSKILSRRN+IRSISRQCDVFINHRG DTKRNIAGLL+DHFSRIGL
Sbjct: 1   MQRYFSTVAKSYLTSSKILSRRNNIRSISRQCDVFINHRGADTKRNIAGLLHDHFSRIGL 60

Query: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM---------- 120
           H FLDSK+MKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM          
Sbjct: 61  HSFLDSKSMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALMMENKKKIIPI 120

Query: 121 --------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVID 177
                   LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDT+NGDWSELLRKASNAVID
Sbjct: 121 FVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVID 180

[Close]

BLAST of PI0028394 vs. ExPASy TrEMBL
Match: A0A5D3DRR9 (Disease resistance-like protein CSA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00490 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 7.9e-89
Identity = 167/195 (85.64%), Postives = 173/195 (88.72%), Query Frame = 0


Query: 1   MQRYFSTLAKNYL-MSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIG 60
           MQRYFST+AKNYL  SSKILSRRNDIRS+SRQCDVFINHRGVDTKRNIAGLL+DHFSRIG
Sbjct: 1   MQRYFSTVAKNYLTSSSKILSRRNDIRSLSRQCDVFINHRGVDTKRNIAGLLHDHFSRIG 60

Query: 61  LHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM--------- 120
           L PFLDSK+MKPGDKLFGEIEEGIRSCKVG+AVFSPRYCESYFCLHELALM         
Sbjct: 61  LRPFLDSKSMKPGDKLFGEIEEGIRSCKVGLAVFSPRYCESYFCLHELALMVENKKKIIP 120

Query: 121 ---------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVI 177
                    LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDT+NGDWSELLRKASNAVI
Sbjct: 121 IFVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVI 180

[Close]

BLAST of PI0028394 vs. ExPASy TrEMBL
Match: A0A1S3BG53 (disease resistance-like protein CSA1 OS=Cucumis melo OX=3656 GN=LOC103489293 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 7.9e-89
Identity = 167/195 (85.64%), Postives = 173/195 (88.72%), Query Frame = 0


Query: 1   MQRYFSTLAKNYL-MSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIG 60
           MQRYFST+AKNYL  SSKILSRRNDIRS+SRQCDVFINHRGVDTKRNIAGLL+DHFSRIG
Sbjct: 1   MQRYFSTVAKNYLTSSSKILSRRNDIRSLSRQCDVFINHRGVDTKRNIAGLLHDHFSRIG 60

Query: 61  LHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM--------- 120
           L PFLDSK+MKPGDKLFGEIEEGIRSCKVG+AVFSPRYCESYFCLHELALM         
Sbjct: 61  LRPFLDSKSMKPGDKLFGEIEEGIRSCKVGLAVFSPRYCESYFCLHELALMVENKKKIIP 120

Query: 121 ---------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVI 177
                    LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDT+NGDWSELLRKASNAVI
Sbjct: 121 IFVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVI 180

[Close]

BLAST of PI0028394 vs. ExPASy TrEMBL
Match: A0A6J1K0B8 (vesicle-associated protein 1-4-like OS=Cucurbita maxima OX=3661 GN=LOC111489938 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.2e-66
Identity = 126/187 (67.38%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 3   RYFSTLAKNY--LMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGL 62
           RY   L +++  ++ S+ILS  +D RSISR CDVFINHRG+DTK+N+AGLL+DH +RIGL
Sbjct: 4   RYVQALQRSFSPVVRSRILSHPSDFRSISRDCDVFINHRGIDTKKNVAGLLFDHLTRIGL 63

Query: 63  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM---------- 122
            PFLDSKNM+PGDKLF EI+EGIR  K+GIAVFSPRYCESY+CLHELA+M          
Sbjct: 64  RPFLDSKNMRPGDKLFDEIKEGIRISKIGIAVFSPRYCESYYCLHELAMMVENKKKIIPI 123

Query: 123 --------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVID 170
                   L V+Y++SCP+ ELQRFNWALGEAKYTVGLTFD+INGDWS+LLRKASNAVID
Sbjct: 124 FVDIRPSELHVKYSHSCPETELQRFNWALGEAKYTVGLTFDSINGDWSKLLRKASNAVID 183

[Close]

BLAST of PI0028394 vs. ExPASy TrEMBL
Match: A0A6J1GSF2 (vesicle-associated protein 1-4-like OS=Cucurbita moschata OX=3662 GN=LOC111456704 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.2e-66
Identity = 130/194 (67.01%), Postives = 151/194 (77.84%), Query Frame = 0


Query: 1   MQRYFSTLAKNYLMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGL 60
           MQR FS + +     S+IL+  +D RSISR C+VFINHRG+DTK+N+AGLL+DH +RIGL
Sbjct: 9   MQRSFSAVVR-----SRILNHPSDFRSISRGCNVFINHRGIDTKKNVAGLLFDHLTRIGL 68

Query: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM---------- 120
            PFLDSKNM+PGDKLF EIEEGIRS K+GIAVFSPRYCESY+CLHELA+M          
Sbjct: 69  RPFLDSKNMRPGDKLFNEIEEGIRSSKIGIAVFSPRYCESYYCLHELAMMVENKKKIIPI 128

Query: 121 --------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVID 177
                   L V+Y++SCP+ ELQRFNWALGEAKYTVGLTFD+INGDWS+LLRKASNAVID
Sbjct: 129 FVDIRPSELHVKYSHSCPENELQRFNWALGEAKYTVGLTFDSINGDWSKLLRKASNAVID 188

[Close]

BLAST of PI0028394 vs. NCBI nr
Match: XP_004146639.1 (TIR domain-containing protein [Cucumis sativus] >KGN64647.1 hypothetical protein Csa_013499 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 1.1e-89
Identity = 167/194 (86.08%), Postives = 173/194 (89.18%), Query Frame = 0


Query: 1   MQRYFSTLAKNYLMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGL 60
           MQRYFST+AK+YL SSKILSRRN+IRSISRQCDVFINHRG DTKRNIAGLL+DHFSRIGL
Sbjct: 1   MQRYFSTVAKSYLTSSKILSRRNNIRSISRQCDVFINHRGADTKRNIAGLLHDHFSRIGL 60

Query: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM---------- 120
           H FLDSK+MKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM          
Sbjct: 61  HSFLDSKSMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALMMENKKKIIPI 120

Query: 121 --------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVID 177
                   LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDT+NGDWSELLRKASNAVID
Sbjct: 121 FVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVID 180

[Close]

BLAST of PI0028394 vs. NCBI nr
Match: XP_008446613.1 (PREDICTED: disease resistance-like protein CSA1 [Cucumis melo] >KAA0056960.1 disease resistance-like protein CSA1 [Cucumis melo var. makuwa] >TYK26387.1 disease resistance-like protein CSA1 [Cucumis melo var. makuwa])

HSP 1 Score: 336.3 bits (861), Expect = 1.6e-88
Identity = 167/195 (85.64%), Postives = 173/195 (88.72%), Query Frame = 0


Query: 1   MQRYFSTLAKNYL-MSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIG 60
           MQRYFST+AKNYL  SSKILSRRNDIRS+SRQCDVFINHRGVDTKRNIAGLL+DHFSRIG
Sbjct: 1   MQRYFSTVAKNYLTSSSKILSRRNDIRSLSRQCDVFINHRGVDTKRNIAGLLHDHFSRIG 60

Query: 61  LHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM--------- 120
           L PFLDSK+MKPGDKLFGEIEEGIRSCKVG+AVFSPRYCESYFCLHELALM         
Sbjct: 61  LRPFLDSKSMKPGDKLFGEIEEGIRSCKVGLAVFSPRYCESYFCLHELALMVENKKKIIP 120

Query: 121 ---------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVI 177
                    LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDT+NGDWSELLRKASNAVI
Sbjct: 121 IFVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVI 180

[Close]

BLAST of PI0028394 vs. NCBI nr
Match: XP_038893500.1 (TIR-only protein-like [Benincasa hispida])

HSP 1 Score: 327.4 bits (838), Expect = 7.6e-86
Identity = 162/194 (83.51%), Postives = 170/194 (87.63%), Query Frame = 0


Query: 1   MQRYFSTLAKNYLMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGL 60
           MQRYFST+AKNYL S KILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHF+RIGL
Sbjct: 1   MQRYFSTVAKNYLTSCKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFTRIGL 60

Query: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM---------- 120
           HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM          
Sbjct: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALMVENKKKIIPI 120

Query: 121 --------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVID 177
                   LRVEY+++CP KELQRFNWALGEAKYTVGLTFD+INGDWSELLRKASNAV+D
Sbjct: 121 FVDVRPSQLRVEYSHNCPDKELQRFNWALGEAKYTVGLTFDSINGDWSELLRKASNAVVD 180

[Close]

BLAST of PI0028394 vs. NCBI nr
Match: KAG7019631.1 (TMV resistance protein N, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 285.8 bits (730), Expect = 2.5e-73
Identity = 141/194 (72.68%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 1   MQRYFSTLAKNYLMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGL 60
           MQRYFS+L +N+L+ S IL+RRN +RS+S++CDVFIN+RGVDT+ +IAGLLYDHF+RIGL
Sbjct: 1   MQRYFSSLPRNHLVPSTILTRRNHVRSMSQRCDVFINYRGVDTRPSIAGLLYDHFTRIGL 60

Query: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM---------- 120
            PFLDS NMKPGDKLFGEIEE IRSCKVGIAVFSPRYC+SYFCLHELALM          
Sbjct: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120

Query: 121 --------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVID 177
                   LRVE+N +CP+KE++RFNWALGEAKYTVGLTFD+INGDWSELLRKAS AVID
Sbjct: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVID 180

[Close]

BLAST of PI0028394 vs. NCBI nr
Match: KAG6584009.1 (TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 282.7 bits (722), Expect = 2.1e-72
Identity = 139/194 (71.65%), Postives = 160/194 (82.47%), Query Frame = 0


Query: 1   MQRYFSTLAKNYLMSSKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFSRIGL 60
           MQRYFS+L +N+L+ S IL+RRN + S+S++CDVFIN+RGVDT+ +IAGLLYDHF+RIGL
Sbjct: 1   MQRYFSSLPRNHLVPSTILTRRNHVGSMSQRCDVFINYRGVDTRPSIAGLLYDHFTRIGL 60

Query: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALM---------- 120
            PFLDS NMKPGDKLFGEIEE IRSCKVGIAVFSPRYC+SYFCLHELALM          
Sbjct: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120

Query: 121 --------LRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTINGDWSELLRKASNAVID 177
                   LRVE+N +CP+KE++RFNWALGEAKYTVGLTFD+INGDWSELLRKAS AV+D
Sbjct: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVVD 180

[Close]

BLAST of PI0028394 vs. TAIR 10
Match: AT1G61105.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.1e-45
Identity = 88/152 (57.89%), Postives = 110/152 (72.37%), Query Frame = 0


Query: 32  CDVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIA 91
           CD+FINHRG+DTK+ I+GLLYD F+R  L  FLDSK++KPGD+LF EI+  I++C VGIA
Sbjct: 29  CDIFINHRGIDTKKTISGLLYDCFTRQRLTAFLDSKSLKPGDRLFVEIDMAIKACGVGIA 88

Query: 92  VFSPRYCESYFCLHELALMLR------------------VEYNYSCPKKELQRFNWALGE 151
           VFSPRYC+SYFCLHELAL+++                  V+ + + P  E++RF  AL E
Sbjct: 89  VFSPRYCDSYFCLHELALLVKNKKRVIPIFCDVKPSELCVKDDKTRPAAEIRRFQLALEE 148

Query: 152 AKYTVGLTFDTINGDWSELLRKASNAVIDNLI 166
           AKYTVGLTFDT NGDWSE L  AS+AV  NL+
Sbjct: 149 AKYTVGLTFDTSNGDWSEFLAMASDAVTKNLL 180

[Close]

BLAST of PI0028394 vs. TAIR 10
Match: AT1G52900.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 157.9 bits (398), Expect = 7.4e-39
Identity = 78/152 (51.32%), Postives = 96/152 (63.16%), Query Frame = 0


Query: 33  DVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAV 92
           DVFINHRG DTKRNIA LLYD+     L PFLDSKNMKPGDKLF  I   I + KV + V
Sbjct: 39  DVFINHRGSDTKRNIATLLYDNLKSRNLRPFLDSKNMKPGDKLFDHINNAILTSKVAVTV 98

Query: 93  FSPRYCESYFCLHELALMLR-------------------VEYNYSCPKKELQRFNWALGE 152
           FSP YC+SYFCLHELAL++                    +    +C   E+QRF WAL E
Sbjct: 99  FSPNYCDSYFCLHELALIMESRKRVIPIFCDIKPSQLDVMIERVTCSDDEIQRFRWALQE 158

Query: 153 AKYTVGLTFDTINGDWSELLRKASNAVIDNLI 166
           AK  VGLTFD+  G+ SE++  AS+ +++ L+
Sbjct: 159 AKDIVGLTFDSYKGNLSEVVTVASDVIVERLV 190

[Close]

BLAST of PI0028394 vs. TAIR 10
Match: AT1G72940.1 (Toll-Interleukin-Resistance (TIR) domain-containing protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-10
Identity = 30/82 (36.59%), Postives = 44/82 (53.66%), Query Frame = 0


Query: 33  DVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAV 92
           DVF++ RGVDT+RN    LY  F R  +  F D K ++ G ++  E++  I   K  + V
Sbjct: 10  DVFLSFRGVDTRRNFISFLYKEFVRRKIRTFKDDKELENGRRISPELKRAIEESKFAVVV 69

Query: 93  FSPRYCESYFCLHELALMLRVE 115
            S  Y  S +CL EL  ++  E
Sbjct: 70  VSVNYAASPWCLDELVKIMDFE 91

[Close]

BLAST of PI0028394 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-10
Identity = 38/138 (27.54%), Postives = 64/138 (46.38%), Query Frame = 0


Query: 33  DVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAV 92
           DVF++ RG D ++N    LYD   R G+  F+D   ++ G+ +  E+   I + K+ I V
Sbjct: 15  DVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVV 74

Query: 93  FSPRYCESYFCLHELALMLRVEYN-----------YSCPKK-ELQRFNWALGEAKYTVGL 152
            +  Y  S +CL EL  +++   N           Y  P     Q+ ++A   +K+    
Sbjct: 75  LTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSH 134

Query: 153 TFDTINGDWSELLRKASN 159
             + +  DW E L K +N
Sbjct: 135 PLNKLK-DWREALTKVAN 151

[Close]

BLAST of PI0028394 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-10
Identity = 38/138 (27.54%), Postives = 64/138 (46.38%), Query Frame = 0


Query: 33  DVFINHRGVDTKRNIAGLLYDHFSRIGLHPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAV 92
           DVF++ RG D ++N    LYD   R G+  F+D   ++ G+ +  E+   I + K+ I V
Sbjct: 18  DVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVV 77

Query: 93  FSPRYCESYFCLHELALMLRVEYN-----------YSCPKK-ELQRFNWALGEAKYTVGL 152
            +  Y  S +CL EL  +++   N           Y  P     Q+ ++A   +K+    
Sbjct: 78  LTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSH 137

Query: 153 TFDTINGDWSELLRKASN 159
             + +  DW E L K +N
Sbjct: 138 PLNKLK-DWREALTKVAN 154

The following BLAST results are available for this feature:

BLAST of PI0028394 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1GTC2	1.6e-30	43.51	TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=...	[more]
A0A290U7C4	1.8e-10	32.95	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
F4KIF3	5.0e-08	40.00	Disease resistance-like protein CSA1 OS=Arabidopsis thaliana OX=3702 GN=CSA1 PE=...	[more]
Q9SZ66	1.5e-07	28.67	Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=...	[more]
Q0WSX8	1.0e-05	35.06	TIR domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=TIK PE=1 SV=1	[more]

BLAST of PI0028394 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LV24	5.5e-90	86.08	TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073070 PE=4 SV...	[more]
A0A5D3DRR9	7.9e-89	85.64	Disease resistance-like protein CSA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BG53	7.9e-89	85.64	disease resistance-like protein CSA1 OS=Cucumis melo OX=3656 GN=LOC103489293 PE=...	[more]
A0A6J1K0B8	3.2e-66	67.38	vesicle-associated protein 1-4-like OS=Cucurbita maxima OX=3661 GN=LOC111489938 ...	[more]
A0A6J1GSF2	3.2e-66	67.01	vesicle-associated protein 1-4-like OS=Cucurbita moschata OX=3662 GN=LOC11145670...	[more]

BLAST of PI0028394 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146639.1	1.1e-89	86.08	TIR domain-containing protein [Cucumis sativus] >KGN64647.1 hypothetical protein...	[more]
XP_008446613.1	1.6e-88	85.64	PREDICTED: disease resistance-like protein CSA1 [Cucumis melo] >KAA0056960.1 dis...	[more]
XP_038893500.1	7.6e-86	83.51	TIR-only protein-like [Benincasa hispida]	[more]
KAG7019631.1	2.5e-73	72.68	TMV resistance protein N, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6584009.1	2.1e-72	71.65	TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of PI0028394 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61105.1	1.1e-45	57.89	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G52900.1	7.4e-39	51.32	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G72940.1	1.5e-10	36.59	Toll-Interleukin-Resistance (TIR) domain-containing protein	[more]
AT5G36930.1	1.9e-10	27.54	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G36930.2	1.9e-10	27.54	Disease resistance protein (TIR-NBS-LRR class) family	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 31..159 e-value: 2.2E-14 score: 63.8
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF13676	TIR_2	coord: 34..112 e-value: 7.4E-13 score: 49.2
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 30..164 score: 17.358591
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 7..136 e-value: 2.1E-24 score: 87.7
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 23..113
None	No IPR available	PANTHER	PTHR31008	COP1-INTERACTING PROTEIN-RELATED	coord: 24..165
None	No IPR available	PANTHER	PTHR31008:SF19	TOLL-INTERLEUKIN-RESISTANCE (TIR) DOMAIN FAMILY PROTEIN	coord: 24..165

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028394.1	PI0028394.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003953	NAD+ nucleosidase activity