PI0028362 (gene) Melon (PI 482460) v1

Overview
NamePI0028362
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionribonuclease 3-like protein 2
Locationchr06: 26401784 .. 26403612 (+)
RNA-Seq ExpressionPI0028362
SyntenyPI0028362
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTTCCCTTCCACTCACTCAGCTCCCAGCTGCGCGTCCTTCTCCATTCTTCAACCATTCACAGTCCAGGGACCGGCGATTCGCCAACCACAGCCGCCGGATTTTAAGGTCTGACCTACGGAATCCTCCATGATAGCATTTTTTTTCTTTTCTTTTCTTTTCTTTTGTTTTTTTTGGTTGAGGTACTCCATGACAGCAATTGAGCGCATACTATCCTATAAGTTTAAGAACAAGAAGCTGTTGGAAGAAGCCCTCACCCACTCCTCCTACCCCAATTCTGCCTCCTATGACCGCCTCGCGTTCATCGGCGATTCAGCTATTGGCCTCGCTATTTCCAATTACATTTTTCCCCTTTATCCGGACCTCGATAAAGGTCAGCTCACTTTACTTAGGGCTGCTAACGTTAGCACCGAGAAGCTTGCTCGTGTTGCAGTCCTCCATGGATTGTATGATTATGTTAGGCGCAACGCAGCCGATCTCGATGATAAGGTTAGAGAGTTTGCCGATGCCGTCGCTCTAGAAGACAACTCAATTCCCTATGGTGGGTTGGTGAAGGCTCCTAAGGTTCTTGCTGACATTGTGGAATCTGTGGCTGGTGCTATCTATGTAGATGTTGAGTTTGACCTTCAAAAACTATGGTCGGTGAGTTCTTAGTTACATTTGAAGTATGATTATTACAAATTTACTAGCTAGACACCAACTAGTTCTCTTTTCTTTTCTTTTTTTTTTCAATCTTTTGTAGATTATTAAGGATTTATTAGAGCCTATCTATACTCCTGAAGACTTAGAGGCAGATCCCCAACCTGTAACTCTGCTCTTTGAAGTTTGCCAAAAAAATGGGAAGCAGGTTGATATTAAGAATCAGGAAAAGGGTTCCAAGAATGTCGCGAGTGTCTATGTTGACGGTACGTTAGTTGCTTCTGGCTTTTCGAAGCAAAGACCAATCGCAAGGCTTAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAAAATTAAAACGGTTGTAACTATTGATGGTATTGATGGTTCGTTTGAAATTGAAGGGGCAAAGCAGAAGCTACATGTTTTTTGTTGCCAGAAAAAGTGGCAGAATCCAATTTACAGGTATATACTATATGATATTTGCGGTGTTGCATACTTGCATTGTATGGACTCTGAATTAGGAGCTAGTTTATATGTTGTAGTTGTGGATTTTTCATTTACAAGCATGTACTCTCATATATTTGCTTTCTTTTCCCCTTCTCCGTGTCCTCCTTTTTCCAACGTGTTCAACCACTAAACACCTTATGGATTTCTTACTTGCAGCATTGAGAAAGTTCTTGGACCCCCACATGAAAGAAGATTTGTTTGCTCTGTTAAAATTCCCACTTATAATGGTACGCACTCTATAATCGGAGATGAGAAGTCAAGAGTAAAAGAGGCAAAGAACTCTGCAGCCTCTTCGATGATTCGCAATTTACAGGAAAGAAAGTATCTGTAATGCATATATTATTCAATTGGGCAGAACCTAGCATAACTGTTTAGTAAGTTTATGCTTTCTGATGCCCTTATTTTCCTTGGATATCTTGGAGGTGGGTATGTTGTAAACCATGATTCTACTTGCCTTTCTGAGTATAAGTTATTGTATCAAAATGTGTATTGATTTCAATGTAAAATTCAAGCCAACCAGTTTAGGCTGTCATGATGTGTAAATCTAATGTATCTGGTTTTCTATTTTGTGCATGTTTGTTTGGAGCAATTTTAGAATTATAGGGATATTGTGGATGGTTGTATTAATTTACAGTCTCAAATTGAAAAGTTTACTTAGTTCTAAA

mRNA sequence

CCCTTCCCTTCCACTCACTCAGCTCCCAGCTGCGCGTCCTTCTCCATTCTTCAACCATTCACAGTCCAGGGACCGGCGATTCGCCAACCACAGCCGCCGGATTTTAAGGTCTGACCTACGGAATCCTCCATGATAGCATTTTTTTTCTTTTCTTTTCTTTTCTTTTGTTTTTTTTGGTTGAGGTACTCCATGACAGCAATTGAGCGCATACTATCCTATAAGTTTAAGAACAAGAAGCTGTTGGAAGAAGCCCTCACCCACTCCTCCTACCCCAATTCTGCCTCCTATGACCGCCTCGCGTTCATCGGCGATTCAGCTATTGGCCTCGCTATTTCCAATTACATTTTTCCCCTTTATCCGGACCTCGATAAAGGTCAGCTCACTTTACTTAGGGCTGCTAACGTTAGCACCGAGAAGCTTGCTCGTGTTGCAGTCCTCCATGGATTGTATGATTATGTTAGGCGCAACGCAGCCGATCTCGATGATAAGGTTAGAGAGTTTGCCGATGCCGTCGCTCTAGAAGACAACTCAATTCCCTATGGTGGGTTGGTGAAGGCTCCTAAGGTTCTTGCTGACATTGTGGAATCTGTGGCTGGTGCTATCTATGTAGATGTTGAGTTTGACCTTCAAAAACTATGGTCGATTATTAAGGATTTATTAGAGCCTATCTATACTCCTGAAGACTTAGAGGCAGATCCCCAACCTGTAACTCTGCTCTTTGAAGTTTGCCAAAAAAATGGGAAGCAGGTTGATATTAAGAATCAGGAAAAGGGTTCCAAGAATGTCGCGAGTGTCTATGTTGACGGTACGTTAGTTGCTTCTGGCTTTTCGAAGCAAAGACCAATCGCAAGGCTTAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAAAATTAAAACGGTTGTAACTATTGATGGTATTGATGGTTCGTTTGAAATTGAAGGGGCAAAGCAGAAGCTACATGTTTTTTGTTGCCAGAAAAAGTGGCAGAATCCAATTTACAGCATTGAGAAAGTTCTTGGACCCCCACATGAAAGAAGATTTGTTTGCTCTGTTAAAATTCCCACTTATAATGGTACGCACTCTATAATCGGAGATGAGAAGTCAAGAGTAAAAGAGGCAAAGAACTCTGCAGCCTCTTCGATGATTCGCAATTTACAGGAAAGAAAGTATCTGTAATGCATATATTATTCAATTGGGCAGAACCTAGCATAACTGTTTAGTAAGTTTATGCTTTCTGATGCCCTTATTTTCCTTGGATATCTTGGAGGTGGGTATGTTGTAAACCATGATTCTACTTGCCTTTCTGAGTATAAGTTATTGTATCAAAATGTGTATTGATTTCAATGTAAAATTCAAGCCAACCAGTTTAGGCTGTCATGATGTGTAAATCTAATGTATCTGGTTTTCTATTTTGTGCATGTTTGTTTGGAGCAATTTTAGAATTATAGGGATATTGTGGATGGTTGTATTAATTTACAGTCTCAAATTGAAAAGTTTACTTAGTTCTAAA

Coding sequence (CDS)

ATGATAGCATTTTTTTTCTTTTCTTTTCTTTTCTTTTGTTTTTTTTGGTTGAGGTACTCCATGACAGCAATTGAGCGCATACTATCCTATAAGTTTAAGAACAAGAAGCTGTTGGAAGAAGCCCTCACCCACTCCTCCTACCCCAATTCTGCCTCCTATGACCGCCTCGCGTTCATCGGCGATTCAGCTATTGGCCTCGCTATTTCCAATTACATTTTTCCCCTTTATCCGGACCTCGATAAAGGTCAGCTCACTTTACTTAGGGCTGCTAACGTTAGCACCGAGAAGCTTGCTCGTGTTGCAGTCCTCCATGGATTGTATGATTATGTTAGGCGCAACGCAGCCGATCTCGATGATAAGGTTAGAGAGTTTGCCGATGCCGTCGCTCTAGAAGACAACTCAATTCCCTATGGTGGGTTGGTGAAGGCTCCTAAGGTTCTTGCTGACATTGTGGAATCTGTGGCTGGTGCTATCTATGTAGATGTTGAGTTTGACCTTCAAAAACTATGGTCGATTATTAAGGATTTATTAGAGCCTATCTATACTCCTGAAGACTTAGAGGCAGATCCCCAACCTGTAACTCTGCTCTTTGAAGTTTGCCAAAAAAATGGGAAGCAGGTTGATATTAAGAATCAGGAAAAGGGTTCCAAGAATGTCGCGAGTGTCTATGTTGACGGTACGTTAGTTGCTTCTGGCTTTTCGAAGCAAAGACCAATCGCAAGGCTTAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAAAATTAAAACGGTTGTAACTATTGATGGTATTGATGGTTCGTTTGAAATTGAAGGGGCAAAGCAGAAGCTACATGTTTTTTGTTGCCAGAAAAAGTGGCAGAATCCAATTTACAGCATTGAGAAAGTTCTTGGACCCCCACATGAAAGAAGATTTGTTTGCTCTGTTAAAATTCCCACTTATAATGGTACGCACTCTATAATCGGAGATGAGAAGTCAAGAGTAAAAGAGGCAAAGAACTCTGCAGCCTCTTCGATGATTCGCAATTTACAGGAAAGAAAGTATCTGTAA

Protein sequence

MIAFFFFSFLFFCFFWLRYSMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDLDKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGGLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEVCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTKIKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTYNGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL
Homology
BLAST of PI0028362 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.2e-94
Identity = 182/337 (54.01%), Postives = 252/337 (74.78%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           SM A+E+IL+YKF NK LL+EA+TH+S  +  SY+RL FIGDSAIGLAISNY++  YP L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDN-SIPYG 139
           +   L+LLRAANVSTEKLARV++ HGLY ++RRNA  LD+KV+EF++AV  ED+ S+ YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 140 GLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFE 199
           GLVKAPKVLAD+ ES+AGA+YVDV FDLQ+LW I + LLEPI T +DL+  PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 200 VCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDT 259
           +C K+ K++DIKN + G+ ++A +Y+D  L+ASG ++ + IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVF-- 298

Query: 260 KIKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIP- 319
            ++ V+  D ++   ++  AK KL+  C +KKW  PIYS+E+       +RFVCS KI  
Sbjct: 299 PVEMVIDEDSVE--IQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 320 TYNGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
           T   T  + GDE+S++K+A++S+A  MIR L++  YL
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRKSHYL 391

BLAST of PI0028362 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 250.8 bits (639), Expect = 2.4e-65
Identity = 139/279 (49.82%), Postives = 187/279 (67.03%), Query Frame = 0

Query: 24  IERILSYKFKNKKLLEEALTHSSYPNSA-SYDRLAFIGDSAIGLAISNYIFPLYPDLDKG 83
           +ER+L Y+F++ +LLEEALTH S+ + A SY RL F+GDSA+GLA SN+++   P L  G
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFADDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGPG 99

Query: 84  QLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALED----NSIPYG 143
            L+ LRAAN+STEKLARVAV H LY  +RRN   LD  V +F + V  E     +++PYG
Sbjct: 100 PLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPYG 159

Query: 144 G-LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLF 203
           G +VKAPKVLADIVE++A A+YVD +FDL+KLW + + L EPI T E +  D QPVT+L 
Sbjct: 160 GSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAETI--DEQPVTMLH 219

Query: 204 EVCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMD 263
           E+CQK+GK    K  +KG   V +V+V G LV  G S+Q+ IA+LNAAR+A  KL+ +  
Sbjct: 220 ELCQKHGKMAQFKTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDATRKLAGAKK 279

Query: 264 TKIKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIY 297
             + T V     D   E+   KQKL+  C ++ W  PI+
Sbjct: 280 QVLTTGVGNGLGDEIGELRECKQKLNEQCSRQNWPKPIF 316

BLAST of PI0028362 vs. ExPASy Swiss-Prot
Match: Q9FKF0 (Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.1e-54
Identity = 135/390 (34.62%), Postives = 203/390 (52.05%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+E+IL+Y F NK LL+EA+T      S  +DRL F GDS + +A +NYI   YP+L
Sbjct: 3   SVEAVEKILNYSFVNKTLLKEAITQ----KSPLFDRLEFFGDSILEVAFTNYICHTYPNL 62

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
              +L  LR ANVS EK AR+AV H L+ ++      L  KV+ FA+AV  ED+ +PYGG
Sbjct: 63  KVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYGG 122

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQ-PVTLLFE 199
           LVKAPK+LAD +ES+A  +++DV +D+++LW I + LLEPIYTP+DL   P+ P   LF 
Sbjct: 123 LVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLFR 182

Query: 200 VCQKNGKQ--------------------------VDIKNQ-------------------- 259
           +  K+G +                           D+K +                    
Sbjct: 183 LADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENSL 242

Query: 260 -----------EKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTKIK 319
                      ++ S NV    V G L    ++++  I   ++      +++       K
Sbjct: 243 TDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVDK 302

Query: 320 TVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVK--IPTY 350
             + ++ ++G       + +L   C + KW  PI+S+++  GP +E +FVCSVK  IP  
Sbjct: 303 DSLHVEPVNG-------RGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNI 362

BLAST of PI0028362 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 194.5 bits (493), Expect = 2.0e-48
Identity = 126/347 (36.31%), Postives = 184/347 (53.03%), Query Frame = 0

Query: 24   IERILSYKFKNKKLLEEALTHSSYPNS--ASYDRLAFIGDSAIGLAISNYIFPLYPDLDK 83
            +ER L Y+FK K LL EA+TH+S P+S  + Y RL F+GD+ +   I+ ++F  Y  L  
Sbjct: 1562 LERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLPP 1621

Query: 84   GQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYG--- 143
            G+LT LRAA V+ E  ARVAV H L+ Y+R  ++ L+ ++REF   V  E +   +    
Sbjct: 1622 GRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSFG 1681

Query: 144  -GLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLF 203
             G  KAPKVL DIVES+AGAI++D   D    W + + LL+P+ TPE L     PV  L 
Sbjct: 1682 LGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRELQ 1741

Query: 204  EVCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMD 263
            E CQ+  + ++ K    G+     V++DG  V    + Q+ +A+  AAR AL  L +   
Sbjct: 1742 ERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEKEI 1801

Query: 264  TKIKTVVTIDGIDGSFEIEG------------AKQKLHVFCCQKKWQNPIYSIEKVLGPP 323
             + K     +G  G  + E              +Q L+  C +K W  P Y   K  GP 
Sbjct: 1802 AESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGGPA 1861

Query: 324  HERRFVCSVKIPTYN--GTHSIIGDEKSRVKEAKNSAASSMIRNLQE 351
            H +RF   V++ T +   T   IG+    VK+AK+SAA  ++  L +
Sbjct: 1862 HAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

BLAST of PI0028362 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 7.7e-48
Identity = 124/333 (37.24%), Postives = 188/333 (56.46%), Query Frame = 0

Query: 24   IERILSYKFKNKKLLEEALTHSSYPNS--ASYDRLAFIGDSAIGLAISNYIFPLYPDLDK 83
            ++ +L  +F+NK LL EA+TH+S P+S  + Y RL F+GD+ +   I+ ++F  Y DL  
Sbjct: 1541 LKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTDLPP 1600

Query: 84   GQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAV---ALEDNSIPYG 143
            G+LT LRAA V+ E  ARVAV H L+ ++R  ++ L+ ++REF   V    L+     +G
Sbjct: 1601 GRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGFNSFG 1660

Query: 144  -GLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLF 203
             G  KAPKVL DIVES+AGAI++D  +D   +W + + LL P+ TPE L     PV  L 
Sbjct: 1661 LGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPM--HPVRELQ 1720

Query: 204  EVCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMD 263
            E CQ+  + ++ K    G+     V+VDG  +    + Q+ +A+  AAR AL+ L +  +
Sbjct: 1721 ERCQQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNALVVLKEK-E 1780

Query: 264  TKIKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIP 323
            T  K     DG   +      +Q L+  C +++W  P Y      GP H +RFV SV++ 
Sbjct: 1781 TATKKEDERDGEKKNGAQMFTRQTLNDICLRRQWPMPQYRCVNEGGPAHAKRFVYSVRVN 1840

Query: 324  TYN--GTHSIIGDEKSRVKEAKNSAASSMIRNL 349
            T +   T   IG+    VK+AK+SAA  ++  L
Sbjct: 1841 TSDRGWTDECIGEPMPSVKKAKDSAAVLLLELL 1870

BLAST of PI0028362 vs. ExPASy TrEMBL
Match: A0A1S3BMA5 (ribonuclease 3-like protein 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491098 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.3e-162
Identity = 300/334 (89.82%), Postives = 313/334 (93.71%), Query Frame = 0

Query: 21  MTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDLD 80
           MTAIER LSYKFKNKKLLE ALTHSSYPN+ASYDRL FIGDSAIGLAISNY F LYPDLD
Sbjct: 1   MTAIERKLSYKFKNKKLLEVALTHSSYPNTASYDRLEFIGDSAIGLAISNYFFSLYPDLD 60

Query: 81  KGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGGL 140
           +G+LTLLRAANVSTEKLARVAVLHGLYDYVRR+AADLDD VREFADAVALEDNSIPYGGL
Sbjct: 61  QGRLTLLRAANVSTEKLARVAVLHGLYDYVRRSAADLDDNVREFADAVALEDNSIPYGGL 120

Query: 141 VKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEVC 200
            KAPKVLADIVESVAGAIYVDVEFDLQKLW+IIKDLL+PIYTPEDLEA+PQPVTLLFEVC
Sbjct: 121 KKAPKVLADIVESVAGAIYVDVEFDLQKLWAIIKDLLKPIYTPEDLEAEPQPVTLLFEVC 180

Query: 201 QKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTKI 260
           QKNGKQV IKNQEKGS NVA VYVD TLVASG SKQ+PIARLNAAR+ALI LSDSMDTKI
Sbjct: 181 QKNGKQVVIKNQEKGSWNVARVYVDRTLVASGSSKQKPIARLNAARQALIILSDSMDTKI 240

Query: 261 KTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTYN 320
           KTVVTIDGIDGSFEIEGA QKLH FC +KKWQNP YS+E+VLG PHERRFVCSVKI TY+
Sbjct: 241 KTVVTIDGIDGSFEIEGATQKLHDFCSRKKWQNPSYSVEEVLGRPHERRFVCSVKISTYD 300

Query: 321 GTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
            THSIIGDEKSRVKEAKNSAAS MIRNLQERKYL
Sbjct: 301 DTHSIIGDEKSRVKEAKNSAASLMIRNLQERKYL 334

BLAST of PI0028362 vs. ExPASy TrEMBL
Match: A0A5D3DKE4 (Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00580 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.2e-144
Identity = 265/335 (79.10%), Postives = 298/335 (88.96%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+ERILSYKFKNKKLLEEALTH SYP+SASY+RL FIGDSAIGLA++NY F  YP+L
Sbjct: 4   SVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAYPNL 63

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
            +GQL+LLRAANVSTEKLARVAV HGLY+YVRRNAA +D KV+EF D+VALE +S+PYGG
Sbjct: 64  HQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVPYGG 123

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEV 199
           L+KAPKVLADIVESVAGA+YVDV FDLQKLW II+ LLEPIYT E+L+ +PQPVT+LF+V
Sbjct: 124 LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFDV 183

Query: 200 CQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTK 259
           CQ+NGKQVDIKN + GSKN+ASVYVDGT VASG S Q+ IARLNAAREALIKLS SMDTK
Sbjct: 184 CQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSMDTK 243

Query: 260 IKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTY 319
           IKTVVTI+GID SFEIEGAKQKLH FCC+KKWQ P YSIEK LGP H RRFVCSVKIPT 
Sbjct: 244 IKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKIPTC 303

Query: 320 NGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
            GT  I+GDEKSRVK+A+NSAAS MIR LQERK+L
Sbjct: 304 YGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of PI0028362 vs. ExPASy TrEMBL
Match: A0A1S3BM12 (ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.2e-144
Identity = 265/335 (79.10%), Postives = 298/335 (88.96%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+ERILSYKFKNKKLLEEALTH SYP+SASY+RL FIGDSAIGLA++NY F  YP+L
Sbjct: 4   SVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAYPNL 63

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
            +GQL+LLRAANVSTEKLARVAV HGLY+YVRRNAA +D KV+EF D+VALE +S+PYGG
Sbjct: 64  HQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVPYGG 123

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEV 199
           L+KAPKVLADIVESVAGA+YVDV FDLQKLW II+ LLEPIYT E+L+ +PQPVT+LF+V
Sbjct: 124 LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFDV 183

Query: 200 CQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTK 259
           CQ+NGKQVDIKN + GSKN+ASVYVDGT VASG S Q+ IARLNAAREALIKLS SMDTK
Sbjct: 184 CQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSMDTK 243

Query: 260 IKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTY 319
           IKTVVTI+GID SFEIEGAKQKLH FCC+KKWQ P YSIEK LGP H RRFVCSVKIPT 
Sbjct: 244 IKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKIPTC 303

Query: 320 NGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
            GT  I+GDEKSRVK+A+NSAAS MIR LQERK+L
Sbjct: 304 YGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of PI0028362 vs. ExPASy TrEMBL
Match: A0A6J1HKS4 (ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.5e-136
Identity = 251/335 (74.93%), Postives = 281/335 (83.88%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+E I+SY FKNKKLLEEALTHSSY  SASY RL FIGDSAIGLA +NY F  YPDL
Sbjct: 4   SVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAYPDL 63

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
            +GQL+LLRAAN+STEKLARVAV HGLY YVRR A  LDDKVREF D+VALE+NS+PYGG
Sbjct: 64  QQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVPYGG 123

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEV 199
           L+KAPKVLADIVESVAGAIYVDV FDLQ+LW II+ LLEPIYTPEDLE +PQPVT+LFEV
Sbjct: 124 LMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVLFEV 183

Query: 200 CQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTK 259
           CQKNGKQVDIKN   GSK V+SV+VDGT VASG S  + IARLNAAREAL KLS +MD  
Sbjct: 184 CQKNGKQVDIKNWSNGSKTVSSVFVDGTFVASGSSTHKEIARLNAAREALAKLSKTMDIN 243

Query: 260 IKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTY 319
           I+T VTIDGID SFEIEGAKQKLH FCC+KKW  P YS EK  GP H++RF CSVKI T 
Sbjct: 244 IRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKISTS 303

Query: 320 NGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
           +G   ++GDEKSRVK+++NSAAS MIR LQE+ YL
Sbjct: 304 SGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

BLAST of PI0028362 vs. ExPASy TrEMBL
Match: A0A6J1F189 (ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 7.3e-134
Identity = 248/335 (74.03%), Postives = 278/335 (82.99%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+E I+SY FKNKKLLEEALTHSSY  SASY RL FIGDSAIGLA +NY F  YPDL
Sbjct: 4   SVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAYPDL 63

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
            +GQL+LLRAAN+STEKLARVAV HGLY YVRR A  LDDKVREF D+VALE+NSIPYGG
Sbjct: 64  QQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSIPYGG 123

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEV 199
           L+KAPKVLADIVESVAGAIYVDV FDLQ+LW II+ LLEPIYTPEDLE +PQPVT+LFEV
Sbjct: 124 LMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVLFEV 183

Query: 200 CQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTK 259
           CQKNGKQVDIKN   GSK V+SV+VDG  VASG S  + IARLNAAREAL K+S +MD  
Sbjct: 184 CQKNGKQVDIKNWSNGSKTVSSVFVDGIFVASGSSTHKVIARLNAAREALAKMSKTMDIN 243

Query: 260 IKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTY 319
           I+  VTIDGID SFEIEGAKQKLH FCC+KKW  P YS EK  GP H++RF CSVKI   
Sbjct: 244 IRAAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKISPP 303

Query: 320 NGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
           +G   ++GDEKSRVK+++NSAAS MIR LQE+ YL
Sbjct: 304 SGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

BLAST of PI0028362 vs. NCBI nr
Match: XP_008449147.1 (PREDICTED: ribonuclease 3-like protein 2 isoform X1 [Cucumis melo])

HSP 1 Score: 582.4 bits (1500), Expect = 2.6e-162
Identity = 300/334 (89.82%), Postives = 313/334 (93.71%), Query Frame = 0

Query: 21  MTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDLD 80
           MTAIER LSYKFKNKKLLE ALTHSSYPN+ASYDRL FIGDSAIGLAISNY F LYPDLD
Sbjct: 1   MTAIERKLSYKFKNKKLLEVALTHSSYPNTASYDRLEFIGDSAIGLAISNYFFSLYPDLD 60

Query: 81  KGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGGL 140
           +G+LTLLRAANVSTEKLARVAVLHGLYDYVRR+AADLDD VREFADAVALEDNSIPYGGL
Sbjct: 61  QGRLTLLRAANVSTEKLARVAVLHGLYDYVRRSAADLDDNVREFADAVALEDNSIPYGGL 120

Query: 141 VKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEVC 200
            KAPKVLADIVESVAGAIYVDVEFDLQKLW+IIKDLL+PIYTPEDLEA+PQPVTLLFEVC
Sbjct: 121 KKAPKVLADIVESVAGAIYVDVEFDLQKLWAIIKDLLKPIYTPEDLEAEPQPVTLLFEVC 180

Query: 201 QKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTKI 260
           QKNGKQV IKNQEKGS NVA VYVD TLVASG SKQ+PIARLNAAR+ALI LSDSMDTKI
Sbjct: 181 QKNGKQVVIKNQEKGSWNVARVYVDRTLVASGSSKQKPIARLNAARQALIILSDSMDTKI 240

Query: 261 KTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTYN 320
           KTVVTIDGIDGSFEIEGA QKLH FC +KKWQNP YS+E+VLG PHERRFVCSVKI TY+
Sbjct: 241 KTVVTIDGIDGSFEIEGATQKLHDFCSRKKWQNPSYSVEEVLGRPHERRFVCSVKISTYD 300

Query: 321 GTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
            THSIIGDEKSRVKEAKNSAAS MIRNLQERKYL
Sbjct: 301 DTHSIIGDEKSRVKEAKNSAASLMIRNLQERKYL 334

BLAST of PI0028362 vs. NCBI nr
Match: XP_031738803.1 (LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus])

HSP 1 Score: 560.1 bits (1442), Expect = 1.4e-155
Identity = 303/356 (85.11%), Postives = 317/356 (89.04%), Query Frame = 0

Query: 1   MIAFF--FFSFLFFCFFWLRYSMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAF 60
           MIAFF  F SFL F FF LR SMT IERILSYKFKNKKLLEEALTHSSY NS SYDRL F
Sbjct: 343 MIAFFFLFLSFLSF-FFLLRNSMTPIERILSYKFKNKKLLEEALTHSSYLNSPSYDRLEF 402

Query: 61  IGDSAIGLAISNYIFPLYPDLDKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLD 120
           IGDSAIGLAIS Y FPLYPDL++GQLTLLRAANVSTEKLARVAVLH LY YVRRNA DLD
Sbjct: 403 IGDSAIGLAISTYFFPLYPDLNQGQLTLLRAANVSTEKLARVAVLHRLYSYVRRNADDLD 462

Query: 121 DKVREFADAVALEDNSIPYGGLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLE 180
           D VREFADAVALEDNSIPYGGLVKAPKVLADIVESVAGAIYVDVEFDLQKLW+IIKD L+
Sbjct: 463 DNVREFADAVALEDNSIPYGGLVKAPKVLADIVESVAGAIYVDVEFDLQKLWAIIKDFLK 522

Query: 181 PIYTPEDLEADPQPVTLLFEVCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRP 240
           PIYTPEDLEADPQPVTLLFE CQK GKQV I+NQEKGS  VA VYVD  LVASG SKQ+P
Sbjct: 523 PIYTPEDLEADPQPVTLLFEDCQKKGKQVHIENQEKGSTYVAKVYVDRRLVASGSSKQKP 582

Query: 241 IARLNAAREALIKLSDSMDTKIKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSI 300
           IARLNAAREALI LSDS DTKIK  VTIDGIDGSFEIEGAKQKLH FCC+KKWQ+P YS 
Sbjct: 583 IARLNAAREALIILSDSTDTKIKRAVTIDGIDGSFEIEGAKQKLHDFCCRKKWQSPSYS- 642

Query: 301 EKVLGPPHERRFVCSVKIPTYNGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
           ++VLG PHERRFVC VKI T+N THSIIGDEKSRVKEA+NSAAS MIR+LQ+RKYL
Sbjct: 643 KEVLGRPHERRFVCCVKISTFNDTHSIIGDEKSRVKEAENSAASLMIRDLQKRKYL 696

BLAST of PI0028362 vs. NCBI nr
Match: KAE8650130.1 (hypothetical protein Csa_010539 [Cucumis sativus])

HSP 1 Score: 553.5 bits (1425), Expect = 1.3e-153
Identity = 288/334 (86.23%), Postives = 302/334 (90.42%), Query Frame = 0

Query: 21  MTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDLD 80
           MT IERILSYKFKNKKLLEEALTHSSY NS SYDRL FIGDSAIGLAIS Y FPLYPDL+
Sbjct: 1   MTPIERILSYKFKNKKLLEEALTHSSYLNSPSYDRLEFIGDSAIGLAISTYFFPLYPDLN 60

Query: 81  KGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGGL 140
           +GQLTLLRAANVSTEKLARVAVLH LY YVRRNA DLDD VREFADAVALEDNSIPYGGL
Sbjct: 61  QGQLTLLRAANVSTEKLARVAVLHRLYSYVRRNADDLDDNVREFADAVALEDNSIPYGGL 120

Query: 141 VKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEVC 200
           VKAPKVLADIVESVAGAIYVDVEFDLQKLW+IIKD L+PIYTPEDLEADPQPVTLLFE C
Sbjct: 121 VKAPKVLADIVESVAGAIYVDVEFDLQKLWAIIKDFLKPIYTPEDLEADPQPVTLLFEDC 180

Query: 201 QKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTKI 260
           QK GKQV I+NQEKGS  VA VYVD  LVASG SKQ+PIARLNAAREALI LSDS DTKI
Sbjct: 181 QKKGKQVHIENQEKGSTYVAKVYVDRRLVASGSSKQKPIARLNAAREALIILSDSTDTKI 240

Query: 261 KTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTYN 320
           K  VTIDGIDGSFEIEGAKQKLH FCC+KKWQ+P YS ++VLG PHERRFVC VKI T+N
Sbjct: 241 KRAVTIDGIDGSFEIEGAKQKLHDFCCRKKWQSPSYS-KEVLGRPHERRFVCCVKISTFN 300

Query: 321 GTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
            THSIIGDEKSRVKEA+NSAAS MIR+LQ+RKYL
Sbjct: 301 DTHSIIGDEKSRVKEAENSAASLMIRDLQKRKYL 333

BLAST of PI0028362 vs. NCBI nr
Match: XP_008449146.1 (PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa] >TYK24086.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 522.7 bits (1345), Expect = 2.5e-144
Identity = 265/335 (79.10%), Postives = 298/335 (88.96%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+ERILSYKFKNKKLLEEALTH SYP+SASY+RL FIGDSAIGLA++NY F  YP+L
Sbjct: 4   SVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAYPNL 63

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
            +GQL+LLRAANVSTEKLARVAV HGLY+YVRRNAA +D KV+EF D+VALE +S+PYGG
Sbjct: 64  HQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVPYGG 123

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEV 199
           L+KAPKVLADIVESVAGA+YVDV FDLQKLW II+ LLEPIYT E+L+ +PQPVT+LF+V
Sbjct: 124 LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFDV 183

Query: 200 CQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTK 259
           CQ+NGKQVDIKN + GSKN+ASVYVDGT VASG S Q+ IARLNAAREALIKLS SMDTK
Sbjct: 184 CQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSMDTK 243

Query: 260 IKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTY 319
           IKTVVTI+GID SFEIEGAKQKLH FCC+KKWQ P YSIEK LGP H RRFVCSVKIPT 
Sbjct: 244 IKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKIPTC 303

Query: 320 NGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
            GT  I+GDEKSRVK+A+NSAAS MIR LQERK+L
Sbjct: 304 YGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of PI0028362 vs. NCBI nr
Match: XP_022965096.1 (ribonuclease 3-like protein 2 [Cucurbita maxima])

HSP 1 Score: 494.6 bits (1272), Expect = 7.2e-136
Identity = 251/335 (74.93%), Postives = 281/335 (83.88%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+E I+SY FKNKKLLEEALTHSSY  SASY RL FIGDSAIGLA +NY F  YPDL
Sbjct: 4   SVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAYPDL 63

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
            +GQL+LLRAAN+STEKLARVAV HGLY YVRR A  LDDKVREF D+VALE+NS+PYGG
Sbjct: 64  QQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVPYGG 123

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEV 199
           L+KAPKVLADIVESVAGAIYVDV FDLQ+LW II+ LLEPIYTPEDLE +PQPVT+LFEV
Sbjct: 124 LMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVLFEV 183

Query: 200 CQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTK 259
           CQKNGKQVDIKN   GSK V+SV+VDGT VASG S  + IARLNAAREAL KLS +MD  
Sbjct: 184 CQKNGKQVDIKNWSNGSKTVSSVFVDGTFVASGSSTHKEIARLNAAREALAKLSKTMDIN 243

Query: 260 IKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIPTY 319
           I+T VTIDGID SFEIEGAKQKLH FCC+KKW  P YS EK  GP H++RF CSVKI T 
Sbjct: 244 IRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKISTS 303

Query: 320 NGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
           +G   ++GDEKSRVK+++NSAAS MIR LQE+ YL
Sbjct: 304 SGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

BLAST of PI0028362 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 346.7 bits (888), Expect = 2.3e-95
Identity = 182/337 (54.01%), Postives = 252/337 (74.78%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           SM A+E+IL+YKF NK LL+EA+TH+S  +  SY+RL FIGDSAIGLAISNY++  YP L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDN-SIPYG 139
           +   L+LLRAANVSTEKLARV++ HGLY ++RRNA  LD+KV+EF++AV  ED+ S+ YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 140 GLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFE 199
           GLVKAPKVLAD+ ES+AGA+YVDV FDLQ+LW I + LLEPI T +DL+  PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 200 VCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDT 259
           +C K+ K++DIKN + G+ ++A +Y+D  L+ASG ++ + IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVF-- 298

Query: 260 KIKTVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVKIP- 319
            ++ V+  D ++   ++  AK KL+  C +KKW  PIYS+E+       +RFVCS KI  
Sbjct: 299 PVEMVIDEDSVE--IQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 320 TYNGTHSIIGDEKSRVKEAKNSAASSMIRNLQERKYL 355
           T   T  + GDE+S++K+A++S+A  MIR L++  YL
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRKSHYL 391

BLAST of PI0028362 vs. TAIR 10
Match: AT5G45150.1 (RNAse THREE-like protein 3 )

HSP 1 Score: 215.3 bits (547), Expect = 7.8e-56
Identity = 135/390 (34.62%), Postives = 203/390 (52.05%), Query Frame = 0

Query: 20  SMTAIERILSYKFKNKKLLEEALTHSSYPNSASYDRLAFIGDSAIGLAISNYIFPLYPDL 79
           S+ A+E+IL+Y F NK LL+EA+T      S  +DRL F GDS + +A +NYI   YP+L
Sbjct: 3   SVEAVEKILNYSFVNKTLLKEAITQ----KSPLFDRLEFFGDSILEVAFTNYICHTYPNL 62

Query: 80  DKGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGG 139
              +L  LR ANVS EK AR+AV H L+ ++      L  KV+ FA+AV  ED+ +PYGG
Sbjct: 63  KVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYGG 122

Query: 140 LVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQ-PVTLLFE 199
           LVKAPK+LAD +ES+A  +++DV +D+++LW I + LLEPIYTP+DL   P+ P   LF 
Sbjct: 123 LVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLFR 182

Query: 200 VCQKNGKQ--------------------------VDIKNQ-------------------- 259
           +  K+G +                           D+K +                    
Sbjct: 183 LADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENSL 242

Query: 260 -----------EKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMDTKIK 319
                      ++ S NV    V G L    ++++  I   ++      +++       K
Sbjct: 243 TDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVDK 302

Query: 320 TVVTIDGIDGSFEIEGAKQKLHVFCCQKKWQNPIYSIEKVLGPPHERRFVCSVK--IPTY 350
             + ++ ++G       + +L   C + KW  PI+S+++  GP +E +FVCSVK  IP  
Sbjct: 303 DSLHVEPVNG-------RGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNI 362

BLAST of PI0028362 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-49
Identity = 126/347 (36.31%), Postives = 184/347 (53.03%), Query Frame = 0

Query: 24   IERILSYKFKNKKLLEEALTHSSYPNS--ASYDRLAFIGDSAIGLAISNYIFPLYPDLDK 83
            +ER L Y+FK K LL EA+TH+S P+S  + Y RL F+GD+ +   I+ ++F  Y  L  
Sbjct: 1562 LERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLPP 1621

Query: 84   GQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYG--- 143
            G+LT LRAA V+ E  ARVAV H L+ Y+R  ++ L+ ++REF   V  E +   +    
Sbjct: 1622 GRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSFG 1681

Query: 144  -GLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLF 203
             G  KAPKVL DIVES+AGAI++D   D    W + + LL+P+ TPE L     PV  L 
Sbjct: 1682 LGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRELQ 1741

Query: 204  EVCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMD 263
            E CQ+  + ++ K    G+     V++DG  V    + Q+ +A+  AAR AL  L +   
Sbjct: 1742 ERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEKEI 1801

Query: 264  TKIKTVVTIDGIDGSFEIEG------------AKQKLHVFCCQKKWQNPIYSIEKVLGPP 323
             + K     +G  G  + E              +Q L+  C +K W  P Y   K  GP 
Sbjct: 1802 AESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGGPA 1861

Query: 324  HERRFVCSVKIPTYN--GTHSIIGDEKSRVKEAKNSAASSMIRNLQE 351
            H +RF   V++ T +   T   IG+    VK+AK+SAA  ++  L +
Sbjct: 1862 HAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

BLAST of PI0028362 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-49
Identity = 126/347 (36.31%), Postives = 184/347 (53.03%), Query Frame = 0

Query: 24   IERILSYKFKNKKLLEEALTHSSYPNS--ASYDRLAFIGDSAIGLAISNYIFPLYPDLDK 83
            +ER L Y+FK K LL EA+TH+S P+S  + Y RL F+GD+ +   I+ ++F  Y  L  
Sbjct: 1563 LERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLPP 1622

Query: 84   GQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYG--- 143
            G+LT LRAA V+ E  ARVAV H L+ Y+R  ++ L+ ++REF   V  E +   +    
Sbjct: 1623 GRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSFG 1682

Query: 144  -GLVKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLF 203
             G  KAPKVL DIVES+AGAI++D   D    W + + LL+P+ TPE L     PV  L 
Sbjct: 1683 LGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRELQ 1742

Query: 204  EVCQKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREALIKLSDSMD 263
            E CQ+  + ++ K    G+     V++DG  V    + Q+ +A+  AAR AL  L +   
Sbjct: 1743 ERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEKEI 1802

Query: 264  TKIKTVVTIDGIDGSFEIEG------------AKQKLHVFCCQKKWQNPIYSIEKVLGPP 323
             + K     +G  G  + E              +Q L+  C +K W  P Y   K  GP 
Sbjct: 1803 AESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGGPA 1862

Query: 324  HERRFVCSVKIPTYN--GTHSIIGDEKSRVKEAKNSAASSMIRNLQE 351
            H +RF   V++ T +   T   IG+    VK+AK+SAA  ++  L +
Sbjct: 1863 HAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1907

BLAST of PI0028362 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 144.1 bits (362), Expect = 2.2e-34
Identity = 91/229 (39.74%), Postives = 133/229 (58.08%), Query Frame = 0

Query: 23  AIERILSYKFKNKKLLEEALTHSSY--PNSASYDRLAFIGDSAIGLAISNYIFPLYPDLD 82
           ++E+IL+YKFK+K LL +A T +SY    S SY+ L  +GDS + + I      LYP   
Sbjct: 31  SLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDSILNMGIIYDFIKLYPKEA 90

Query: 83  KGQLTLLRAANVSTEKLARVAVLHGLYDYVRRNAADLDDKVREFADAVALEDNSIPYGGL 142
            G LT LRA NV TEKLARVAV H LY Y+R     L++++ EF +  A+E   +   GL
Sbjct: 91  PGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVE--AMEKYPLHSNGL 150

Query: 143 VKAPKVLADIVESVAGAIYVDVEFDLQKLWSIIKDLLEPIYTPEDLEADPQPVTLLFEVC 202
           +K PKVLADIVES  GAI++D     + +W +IK LLEPI   + +++   P+T L E+C
Sbjct: 151 LKVPKVLADIVESTIGAIFMDCN-STETVWKVIKPLLEPIIHLDKMKS--HPMTELNEMC 210

Query: 203 QKNGKQVDIKNQEKGSKNVASVYVDGTLVASGFSKQRPIARLNAAREAL 250
           QK   ++  K+  + ++       D  +       ++  AR  AA+ A+
Sbjct: 211 QKRNLKLTSKDTWEENQTYCFHIEDKFVGCGHHPVKKETARNFAAKNAI 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTQ03.2e-9454.01Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1[more]
Q6ATG62.4e-6549.82Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0... [more]
Q9FKF01.1e-5434.62Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1[more]
Q9SP322.0e-4836.31Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV... [more]
Q8LMR27.7e-4837.24Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... [more]
Match NameE-valueIdentityDescription
A0A1S3BMA51.3e-16289.82ribonuclease 3-like protein 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491098... [more]
A0A5D3DKE41.2e-14479.10Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BM121.2e-14479.10ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1[more]
A0A6J1HKS43.5e-13674.93ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 S... [more]
A0A6J1F1897.3e-13474.03ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4... [more]
Match NameE-valueIdentityDescription
XP_008449147.12.6e-16289.82PREDICTED: ribonuclease 3-like protein 2 isoform X1 [Cucumis melo][more]
XP_031738803.11.4e-15585.11LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus][more]
KAE8650130.11.3e-15386.23hypothetical protein Csa_010539 [Cucumis sativus][more]
XP_008449146.12.5e-14479.10PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclea... [more]
XP_022965096.17.2e-13674.93ribonuclease 3-like protein 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G20420.12.3e-9554.01RNAse THREE-like protein 2 [more]
AT5G45150.17.8e-5634.62RNAse THREE-like protein 3 [more]
AT1G01040.11.4e-4936.31dicer-like 1 [more]
AT1G01040.21.4e-4936.31dicer-like 1 [more]
AT4G15417.12.2e-3439.74RNAse II-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000999Ribonuclease III domainSMARTSM00535riboneu5coord: 36..186
e-value: 1.0E-32
score: 124.7
IPR000999Ribonuclease III domainPFAMPF00636Ribonuclease_3coord: 54..161
e-value: 3.7E-21
score: 75.7
IPR000999Ribonuclease III domainPROSITEPS50142RNASE_3_2coord: 21..163
score: 35.475185
IPR000999Ribonuclease III domainCDDcd00593RIBOccoord: 36..186
e-value: 5.49345E-40
score: 135.818
IPR014720Double-stranded RNA-binding domainSMARTSM00358DRBM_3coord: 192..253
e-value: 0.015
score: 22.3
coord: 278..349
e-value: 3.4E-8
score: 43.2
IPR014720Double-stranded RNA-binding domainPFAMPF00035dsrmcoord: 278..348
e-value: 4.3E-14
score: 53.0
coord: 192..252
e-value: 1.4E-5
score: 25.7
IPR014720Double-stranded RNA-binding domainPROSITEPS50137DS_RBDcoord: 191..254
score: 9.197882
IPR014720Double-stranded RNA-binding domainPROSITEPS50137DS_RBDcoord: 277..350
score: 10.848671
NoneNo IPR availableGENE3D3.30.160.20coord: 275..351
e-value: 1.2E-16
score: 62.6
NoneNo IPR availableGENE3D3.30.160.20coord: 188..263
e-value: 1.2E-7
score: 33.5
NoneNo IPR availablePANTHERPTHR14950:SF49RIBONUCLEASE 3-LIKE PROTEIN 2-RELATEDcoord: 22..345
NoneNo IPR availablePANTHERPTHR14950DICER-RELATEDcoord: 22..345
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 277..351
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 145..252
IPR036389Ribonuclease III, endonuclease domain superfamilyGENE3D1.10.1520.10Ribonuclease III domaincoord: 17..186
e-value: 6.5E-47
score: 161.7
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILY69065RNase III domain-likecoord: 15..182

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028362.1PI0028362.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005978 glycogen biosynthetic process
biological_process GO:0030422 production of siRNA involved in RNA interference
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016075 rRNA catabolic process
biological_process GO:0006396 RNA processing
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003844 1,4-alpha-glucan branching enzyme activity
molecular_function GO:0043169 cation binding
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding