PI0028297 (gene) Melon (PI 482460) v1

Overview
NamePI0028297
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionprotein NIM1-INTERACTING 1-like
Locationchr06: 15950061 .. 15950456 (-)
RNA-Seq ExpressionPI0028297
SyntenyPI0028297
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAGGAAGAAAAAAAAGAAAATTACAGAATAATGAGAAAGAAGAAGAGGAAGAAAATGAAGAAGAAGAAGAGAAAAAGATGGAGTTGTTCTATGCTTTGATTCAAAATACAAAGGCCATGAGAGACAGAATGAAATATTCAAAAGAATTAACAGAAGAAGAGAAATCAAAAGGGGTTTGGAATCCAAAGTTTCAACCACAAGATTTCATTGAAGAAGATGGTTATTACAATAATAAATCCAACATAACTCCTCTTCAATTGTCAGCTGCTTCATCTTCAACAACAAAACAGAGCAATGAACAGAAACAACAACAAGAACAAGATGAAGATGAAGATGAATATAAGGTAGGAAAAGGAAAAAATGGGCTTGACCTTAATCTCTCTCTTTGA

mRNA sequence

ATGGAAGGAGGAAGAAAAAAAAGAAAATTACAGAATAATGAGAAAGAAGAAGAGGAAGAAAATGAAGAAGAAGAAGAGAAAAAGATGGAGTTGTTCTATGCTTTGATTCAAAATACAAAGGCCATGAGAGACAGAATGAAATATTCAAAAGAATTAACAGAAGAAGAGAAATCAAAAGGGGTTTGGAATCCAAAGTTTCAACCACAAGATTTCATTGAAGAAGATGGTTATTACAATAATAAATCCAACATAACTCCTCTTCAATTGTCAGCTGCTTCATCTTCAACAACAAAACAGAGCAATGAACAGAAACAACAACAAGAACAAGATGAAGATGAAGATGAATATAAGGTAGGAAAAGGAAAAAATGGGCTTGACCTTAATCTCTCTCTTTGA

Coding sequence (CDS)

ATGGAAGGAGGAAGAAAAAAAAGAAAATTACAGAATAATGAGAAAGAAGAAGAGGAAGAAAATGAAGAAGAAGAAGAGAAAAAGATGGAGTTGTTCTATGCTTTGATTCAAAATACAAAGGCCATGAGAGACAGAATGAAATATTCAAAAGAATTAACAGAAGAAGAGAAATCAAAAGGGGTTTGGAATCCAAAGTTTCAACCACAAGATTTCATTGAAGAAGATGGTTATTACAATAATAAATCCAACATAACTCCTCTTCAATTGTCAGCTGCTTCATCTTCAACAACAAAACAGAGCAATGAACAGAAACAACAACAAGAACAAGATGAAGATGAAGATGAATATAAGGTAGGAAAAGGAAAAAATGGGCTTGACCTTAATCTCTCTCTTTGA

Protein sequence

MEGGRKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEEEKSKGVWNPKFQPQDFIEEDGYYNNKSNITPLQLSAASSSTTKQSNEQKQQQEQDEDEDEYKVGKGKNGLDLNLSL
Homology
BLAST of PI0028297 vs. ExPASy TrEMBL
Match: A0A0A0LJ23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034770 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 8.0e-38
Identity = 101/132 (76.52%), Postives = 109/132 (82.58%), Query Frame = 0

Query: 1   MEGGRKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEEEKSKG 60
           ME GRK+RKLQ       EE EEEE++KMELFYALIQNTKAMRD MKYSKELTEEEKSKG
Sbjct: 1   MESGRKRRKLQ-------EEEEEEEDEKMELFYALIQNTKAMRDGMKYSKELTEEEKSKG 60

Query: 61  VWNPKFQPQDFIEEDGY-YNNKSNITPLQLSAASSSTTKQSNEQKQQQEQDEDEDEYKVG 120
           VWNPKFQP+DF EEDGY YNNKSNI PLQLSAASSSTTKQ NE+KQQ    E++  YKV 
Sbjct: 61  VWNPKFQPEDFNEEDGYNYNNKSNIIPLQLSAASSSTTKQFNEKKQQ----EEDKHYKVV 120

Query: 121 KGKNGLDLNLSL 132
           + K GLDLNLSL
Sbjct: 121 EVKKGLDLNLSL 121

BLAST of PI0028297 vs. ExPASy TrEMBL
Match: A0A6J1DQG7 (LOW QUALITY PROTEIN: uncharacterized protein LOC111023284 OS=Momordica charantia OX=3673 GN=LOC111023284 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.1e-18
Identity = 73/131 (55.73%), Postives = 99/131 (75.57%), Query Frame = 0

Query: 1   MEGGRKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEEEKSKG 60
           ME  R+KRKLQ++EKE ++E+EEEEE KM+LF+ALIQNTK++RDR+K  +   +++KSKG
Sbjct: 1   MESVRRKRKLQDDEKEAQKEDEEEEE-KMKLFFALIQNTKSIRDRVKSKQSAPQQDKSKG 60

Query: 61  VWNPKFQPQDFIEEDGYYNNKSNITPLQLSAASSSTTKQSNEQKQQQEQDEDEDEYKVGK 120
           VWNPKFQP+DFIE+    + KSN TP     A+SS+TKQ N+Q +++EQ +D+ E     
Sbjct: 61  VWNPKFQPEDFIED---CDKKSNPTP---QFAASSSTKQRNKQ-EEEEQGKDKVE----- 118

Query: 121 GKNGLDLNLSL 132
           G   LDLNLSL
Sbjct: 121 GIEDLDLNLSL 118

BLAST of PI0028297 vs. ExPASy TrEMBL
Match: A0A5C7GWM2 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_024772 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.9e-11
Identity = 62/162 (38.27%), Postives = 88/162 (54.32%), Query Frame = 0

Query: 5   RKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYS--------------- 64
           RKKRK++N    EEEE EE EE+KME FYAL++NTK +RDR++ S               
Sbjct: 9   RKKRKMEN----EEEEEEENEEEKMEQFYALLRNTKDVRDRLRSSSTSSTGLHGSPIPSI 68

Query: 65  ----------------KELTEEEKSKGVWNPKFQPQDFIEEDGYY--NNKSNITPLQLSA 124
                           K+  EE+    VWNP FQP+DF+E+      +N  N+     + 
Sbjct: 69  PSMEINNNNNKQDQEKKKQLEEKALSAVWNPSFQPEDFMEDRNVIAKSNTVNLGSTSSTQ 128

Query: 125 ASSSTTKQSNEQKQQQEQDEDEDEYKVGKGKN--GLDLNLSL 132
            ++S +++ NE+ QQQ+Q E++D+   GK  N   LDLNLSL
Sbjct: 129 LAASASQEKNEEPQQQQQQEEDDKKDGGKDNNNLALDLNLSL 166

BLAST of PI0028297 vs. ExPASy TrEMBL
Match: A0A2I4EAN7 (protein NIM1-INTERACTING 3-like OS=Juglans regia OX=51240 GN=LOC108987874 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 7.1e-10
Identity = 66/142 (46.48%), Postives = 86/142 (60.56%), Query Frame = 0

Query: 1   MEGGRKKRKLQNNE-KEEEEENEEEEEKKMELFYALIQNTKAMRDRM---------KYSK 60
           MEG  KKRK+ N E   EEEE EE+EEKKME F+ALI++T+ +RDR+         K  K
Sbjct: 1   MEGKGKKRKMDNEEGGGEEEEEEEDEEKKMEKFFALIRSTRDVRDRLRTGVNESKDKEEK 60

Query: 61  ELTEEE-KSKGVWNPKFQPQDFIEEDGYYNNKSNITPLQLSAASSSTTKQSNEQKQQQEQ 120
              E+E K+  VWNPKFQP+DF    G Y  KS+ TP     AS+       E+K+++E+
Sbjct: 61  RPVEKEIKTTAVWNPKFQPEDF---PGEYKPKSS-TP----GASTPPGPSKREEKEEEEE 120

Query: 121 DEDEDEYKVGKGKNGLDLNLSL 132
           +ED  + K      GLDLNLSL
Sbjct: 121 EEDRGDDK------GLDLNLSL 128

BLAST of PI0028297 vs. ExPASy TrEMBL
Match: A0A061F4Z3 (Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_026807 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 9.3e-10
Identity = 62/146 (42.47%), Postives = 85/146 (58.22%), Query Frame = 0

Query: 1   MEGGRKKRKLQN------NEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKY----SK 60
           ME GRKKRK+ N      +E+EEEE++EEEEE+K+E F+ALI++T+ MRDR++     SK
Sbjct: 1   MESGRKKRKMGNQEEEEDDEEEEEEDDEEEEEEKVEKFFALIRSTREMRDRLRNVPNGSK 60

Query: 61  ELTEEEKSK-----GVWNPKFQPQDFIEEDGYYNNKSNITPLQLSAASSSTTKQSNEQKQ 120
           E  E +K +      VWNPKFQP+DF+E+           P  L A + +    + EQ+Q
Sbjct: 61  EEEERKKQEEKQVAAVWNPKFQPEDFMEDS---------KPKNLLAVNEAGPSSNREQEQ 120

Query: 121 QQEQDEDEDEYKVGKGKNGLDLNLSL 132
            Q+  E       G    GLDL LSL
Sbjct: 121 GQDTKE-------GGEDGGLDLKLSL 130

BLAST of PI0028297 vs. NCBI nr
Match: KGN61024.1 (hypothetical protein Csa_021295 [Cucumis sativus])

HSP 1 Score: 166.4 bits (420), Expect = 1.7e-37
Identity = 101/132 (76.52%), Postives = 109/132 (82.58%), Query Frame = 0

Query: 1   MEGGRKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEEEKSKG 60
           ME GRK+RKLQ       EE EEEE++KMELFYALIQNTKAMRD MKYSKELTEEEKSKG
Sbjct: 1   MESGRKRRKLQ-------EEEEEEEDEKMELFYALIQNTKAMRDGMKYSKELTEEEKSKG 60

Query: 61  VWNPKFQPQDFIEEDGY-YNNKSNITPLQLSAASSSTTKQSNEQKQQQEQDEDEDEYKVG 120
           VWNPKFQP+DF EEDGY YNNKSNI PLQLSAASSSTTKQ NE+KQQ    E++  YKV 
Sbjct: 61  VWNPKFQPEDFNEEDGYNYNNKSNIIPLQLSAASSSTTKQFNEKKQQ----EEDKHYKVV 120

Query: 121 KGKNGLDLNLSL 132
           + K GLDLNLSL
Sbjct: 121 EVKKGLDLNLSL 121

BLAST of PI0028297 vs. NCBI nr
Match: XP_038889660.1 (protein NIM1-INTERACTING 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 126.7 bits (317), Expect = 1.5e-25
Identity = 84/119 (70.59%), Postives = 97/119 (81.51%), Query Frame = 0

Query: 1   MEGGRKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEEEKSKG 60
           ME GRK+RKL     EEEEE +++EE +M+L +ALIQN KAMR RMK SKE  EEEKSKG
Sbjct: 1   MERGRKRRKL-----EEEEEEDDDEEGEMKLVFALIQNIKAMRGRMK-SKESPEEEKSKG 60

Query: 61  VWNPKFQPQDFIEEDGYYNNKSNITPLQLSAASSSTTKQSNEQKQQQEQDEDEDEYKVG 120
           VWNPKFQP+DFI EDGYYNNKSNIT LQL AASSSTTK SN +K+QQEQ   +D++KVG
Sbjct: 61  VWNPKFQPEDFI-EDGYYNNKSNIT-LQLPAASSSTTKHSNPKKEQQEQ---QDKHKVG 108

BLAST of PI0028297 vs. NCBI nr
Match: XP_038889662.1 (protein NIM1-INTERACTING 3 isoform X3 [Benincasa hispida])

HSP 1 Score: 123.2 bits (308), Expect = 1.6e-24
Identity = 80/111 (72.07%), Postives = 91/111 (81.98%), Query Frame = 0

Query: 1   MEGGRKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEEEKSKG 60
           ME GRK+RKL     EEEEE +++EE +M+L +ALIQN KAMR RMK SKE  EEEKSKG
Sbjct: 1   MERGRKRRKL-----EEEEEEDDDEEGEMKLVFALIQNIKAMRGRMK-SKESPEEEKSKG 60

Query: 61  VWNPKFQPQDFIEEDGYYNNKSNITPLQLSAASSSTTKQSNEQKQQQEQDE 112
           VWNPKFQP+DFI EDGYYNNKSNIT LQL AASSSTTK SN +K+QQEQ +
Sbjct: 61  VWNPKFQPEDFI-EDGYYNNKSNIT-LQLPAASSSTTKHSNPKKEQQEQQD 103

BLAST of PI0028297 vs. NCBI nr
Match: XP_038889661.1 (protein NIM1-INTERACTING 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 123.2 bits (308), Expect = 1.6e-24
Identity = 80/111 (72.07%), Postives = 91/111 (81.98%), Query Frame = 0

Query: 1   MEGGRKKRKLQNNEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEEEKSKG 60
           ME GRK+RKL     EEEEE +++EE +M+L +ALIQN KAMR RMK SKE  EEEKSKG
Sbjct: 1   MERGRKRRKL-----EEEEEEDDDEEGEMKLVFALIQNIKAMRGRMK-SKESPEEEKSKG 60

Query: 61  VWNPKFQPQDFIEEDGYYNNKSNITPLQLSAASSSTTKQSNEQKQQQEQDE 112
           VWNPKFQP+DFI EDGYYNNKSNIT LQL AASSSTTK SN +K+QQEQ +
Sbjct: 61  VWNPKFQPEDFI-EDGYYNNKSNIT-LQLPAASSSTTKHSNPKKEQQEQQD 103

BLAST of PI0028297 vs. NCBI nr
Match: KAG6599168.1 (hypothetical protein SDJN03_08946, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 118.2 bits (295), Expect = 5.2e-23
Identity = 76/141 (53.90%), Postives = 107/141 (75.89%), Query Frame = 0

Query: 1   MEGGRKKRKLQN-----NEKEEEEENEEEEEKKMELFYALIQNTKAMRDRMKYSKELTEE 60
           MEGGRK+RKLQN     +++EE+E+ +E+EE+KM+LF+ALIQNTKAMRD++K  +   E+
Sbjct: 1   MEGGRKRRKLQNIEEDDDDEEEDEDEDEDEEEKMKLFFALIQNTKAMRDKIKSKESAEED 60

Query: 61  EKSKGVWNPKFQPQDFIEEDGYYNNKS-NITPLQLSAASSSTTKQSNEQKQQQE----QD 120
             +KGVWNPKFQP+DFI EDG+  +KS +   ++L AASSS TKQSN++++ QE    Q+
Sbjct: 61  NPTKGVWNPKFQPEDFI-EDGFDTHKSKSKAAIELGAASSSATKQSNQKEEHQEHQEHQE 120

Query: 121 EDEDEYKVGKGKNGLDLNLSL 132
             E + ++ + K GLDLNLSL
Sbjct: 121 HQEHQEQLEEPKKGLDLNLSL 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LJ238.0e-3876.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034770 PE=3 SV=1[more]
A0A6J1DQG71.1e-1855.73LOW QUALITY PROTEIN: uncharacterized protein LOC111023284 OS=Momordica charantia... [more]
A0A5C7GWM24.9e-1138.27Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_024772 PE=3 SV=1[more]
A0A2I4EAN77.1e-1046.48protein NIM1-INTERACTING 3-like OS=Juglans regia OX=51240 GN=LOC108987874 PE=3 S... [more]
A0A061F4Z39.3e-1042.47Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_026807 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KGN61024.11.7e-3776.52hypothetical protein Csa_021295 [Cucumis sativus][more]
XP_038889660.11.5e-2570.59protein NIM1-INTERACTING 3 isoform X1 [Benincasa hispida][more]
XP_038889662.11.6e-2472.07protein NIM1-INTERACTING 3 isoform X3 [Benincasa hispida][more]
XP_038889661.11.6e-2472.07protein NIM1-INTERACTING 3 isoform X2 [Benincasa hispida][more]
KAG6599168.15.2e-2353.90hypothetical protein SDJN03_08946, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..103
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 104..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..131
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availablePANTHERPTHR33669:SF4PROTEIN NIM1-INTERACTING 3coord: 4..131
IPR031425NPR1/NH1-interacting proteinPFAMPF15699NPR1_interactcoord: 22..128
e-value: 2.1E-19
score: 70.1
IPR031425NPR1/NH1-interacting proteinPANTHERPTHR33669PROTEIN NEGATIVE REGULATOR OF RESISTANCEcoord: 4..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028297.1PI0028297.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010112 regulation of systemic acquired resistance
cellular_component GO:0005634 nucleus