PI0028294 (gene) Melon (PI 482460) v1

BLAST of PI0028294 vs. ExPASy Swiss-Prot
Match: Q9XHP0 (11S globulin seed storage protein 2 OS=Sesamum indicum OX=4182 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.1e-115
Identity = 213/465 (45.81%), Postives = 303/465 (65.16%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           +A K +L + L L     +VS   A  R     + QQCR  RI    PS R++SEGG TE
Sbjct: 2   VAFKFLLALSLSL-----LVSAAIAQTREPRLTQGQQCRFQRISGAQPSLRIQSEGGTTE 61

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           +WDE  E+FQCAG+ A R+ IRPN LSLP +H SP L YIE+G+G + +  PGC  ETY+
Sbjct: 62  LWDERQEQFQCAGIVAMRSTIRPNGLSLPNYHPSPRLVYIERGQGLISIMVPGC-AETYQ 121

Query: 121 AQSAQLSRSSRRIRVDKEEDK------HQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVV 180
              +Q  R+  R    +++D+      HQKV R+RQGD++ IP+G   WCYND  +DLV 
Sbjct: 122 VHRSQ--RTMERTEASEQQDRGSVRDLHQKVHRLRQGDIVAIPSGAAHWCYNDGSEDLVA 181

Query: 181 VAFMDLNNDDNQLDLRVRSSYLAGGVPREARRVSKS-DDFVNIFSGFDKEFLEEAYNIPS 240
           V+  D+N+  NQLD + R+ YLAGGVPR   +  ++   F NIF  FD E L EA+N+P 
Sbjct: 182 VSINDVNHLSNQLDQKFRAFYLAGGVPRSGEQEQQARQTFHNIFRAFDAELLSEAFNVPQ 241

Query: 241 DLARKMQ-EERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQ 300
           +  R+MQ EE   GLIV   E M+F+ P+EEE    G    R  +  NGLEET CT + +
Sbjct: 242 ETIRRMQSEEEERGLIVMARERMTFVRPDEEE----GEQEHRGRQLDNGLEETFCTMKFR 301

Query: 301 HNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLA 360
            N+ ++READ +SR+AGRV+++++ KLPIL++M +SAEKG+L+ NA  +  WSMT H + 
Sbjct: 302 TNVESRREADIFSRQAGRVHVVDRNKLPILKYMDLSAEKGNLYSNALVSPDWSMTGHTIV 361

Query: 361 YVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMK 420
           YV  G+A++++ +  G  +  ++V++G MF++PQ+Y S ARAG  GFEW+ FKT+  PM+
Sbjct: 362 YVTRGDAQVQVVDHNGQALMNDRVNQGEMFVVPQYYTSTARAGNNGFEWVAFKTTGSPMR 421

Query: 421 SPVAGHTSFFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPP 458
           SP+AG+TS  RA+PLQ++  ++QI+  +AQ LK  R   +FL  P
Sbjct: 422 SPLAGYTSVIRAMPLQVITNSYQISPNQAQALKMNRGSQSFLLSP 454

BLAST of PI0028294 vs. ExPASy Swiss-Prot
Match: B5KVH4 (11S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.8e-89
Identity = 188/506 (37.15%), Postives = 280/506 (55.34%), Query Frame = 0

Query: 1   MATKVVLGILLCLF---AFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGG 60
           MA  ++L I LCL     F   ++     ++H F     QC+L+R+ A  P+ R+E+E G
Sbjct: 1   MAKPILLSIYLCLIIVALFNGCLAQSGGRQQHKF----GQCQLNRLDALEPTNRIEAEAG 60

Query: 61  ITEVWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVE 120
           + E WD  +++ QCAGVA  R  I PN L LP + ++P L YI +G G  G+ FPGC  E
Sbjct: 61  VIESWDPNHQQLQCAGVAVVRRTIEPNGLLLPHYSNAPQLVYIARGRGITGVLFPGC-PE 120

Query: 121 TYEAQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAF 180
           T+E    Q  +  RR   + ++D+HQK+R  R+GD+I  PAG   WCYND    +V +  
Sbjct: 121 TFEESQRQSQQGQRR---EFQQDRHQKIRHFREGDIIAFPAGVAHWCYNDGSSPVVAIFL 180

Query: 181 MDLNNDDNQLDLRVRSSYLAGGVPREARRVSKS-----------------------DDFV 240
           +D +N+ NQLD   R+ YLAG    E R   +                        D   
Sbjct: 181 LDTHNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRRGEHGEQQRDLGN 240

Query: 241 NIFSGFDKEFLEEAYNIPSDLARKMQEERS-AGLIVKCD-EEMSFMTP-----EEEEEEL 300
           N+FSGFD EFL +A+N+ ++ AR++Q E    G IV+ +  ++  + P     E+E EE 
Sbjct: 241 NVFSGFDAEFLADAFNVDTETARRLQSENDHRGSIVRVEGRQLQVIRPRWSREEQEHEER 300

Query: 301 SGSPFSRREE-----------DLNGLEETICTARVQHNMNTQREADAYSREAGRVNILNQ 360
                 R  E           D NGLEETICT  ++ N+     AD Y+ EAGR++ +N 
Sbjct: 301 KERERERESESERRQSRRGGRDDNGLEETICTLSLRENIGDPSRADIYTEEAGRISTVNS 360

Query: 361 LKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAEIEIANDYGNQVFKEKV 420
             LPILR++ +SAE+G L+ +A Y  HW++  H + Y + G AE+++ +++G  VF +++
Sbjct: 361 HNLPILRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFDDEL 420

Query: 421 SRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTSFFRALPLQLLEQAFQI 463
             G +  IPQ +    RA  +GFEW++FKT+   M SP+AG TS  RALP ++L  AFQI
Sbjct: 421 REGQLLTIPQNFAVVKRARDEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLVNAFQI 480

BLAST of PI0028294 vs. ExPASy Swiss-Prot
Match: Q8GZP6 (11S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occidentale OX=171929 PE=1 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 9.0e-89
Identity = 174/441 (39.46%), Postives = 265/441 (60.09%), Query Frame = 0

Query: 25  APERHWFREEAQQCRLDRIQARPPSRRVESEGGITEVWDEANEEFQCAGVAAFRNIIRPN 84
           A  + W  ++  +C++DR+ A  P  RVE E G  E WD  +E+F+CAGVA  R+ I+PN
Sbjct: 14  ASRQEW--QQQDECQIDRLDALEPDNRVEYEAGTVEAWDPNHEQFRCAGVALVRHTIQPN 73

Query: 85  SLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYEA--QSAQLSRSSRRIRVDKEEDKH 144
            L LP++ ++P L Y+ QGEG  G+++PGC  ETY+A  Q  Q  +S R       +D+H
Sbjct: 74  GLLLPQYSNAPQLIYVVQGEGMTGISYPGC-PETYQAPQQGRQQGQSGR------FQDRH 133

Query: 145 QKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMDLNNDDNQLDLRVRSSYLAGGVPR 204
           QK+RR R+GD+I IPAG   WCYN+    +V V  +D++N  NQLD   R  +LAG  P+
Sbjct: 134 QKIRRFRRGDIIAIPAGVAHWCYNEGNSPVVTVTLLDVSNSQNQLDRTPRKFHLAGN-PK 193

Query: 205 EA--RRVSKSDDFVNIFSGFDKEFLEEAYNIPSDLARKMQEERSAGLIVKC-DEEMSFMT 264
           +   ++        N+FSGFD E L EA+ +   L ++++ E + G IVK  D+E+  + 
Sbjct: 194 DVFQQQQQHQSRGRNLFSGFDTELLAEAFQVDERLIKQLKSEDNRGGIVKVKDDELRVIR 253

Query: 265 PEEEEEELSGSPFSRREEDL--------NGLEETICTARVQHNMNTQREADAYSREAGRV 324
           P   + E  GS      ED         NG+EETICT R++ N+N    AD Y+ E GR+
Sbjct: 254 PSRSQSE-RGSESEEESEDEKRRWGQRDNGIEETICTMRLKENINDPARADIYTPEVGRL 313

Query: 325 NILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAEIEIANDYGNQV 384
             LN L LPIL+++ +S EKG L+ NA    HW++  H + Y  +G+ ++++ +++GN+V
Sbjct: 314 TTLNSLNLPILKWLQLSVEKGVLYKNALVLPHWNLNSHSIIYGCKGKGQVQVVDNFGNRV 373

Query: 385 FKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTSFFRALPLQLLE 444
           F  +V  G M ++PQ +    RA  + FEWI+FKT+++ M SP+AG TS    +P ++L 
Sbjct: 374 FDGEVREGQMLVVPQNFAVVKRAREERFEWISFKTNDRAMTSPLAGRTSVLGGMPEEVLA 433

Query: 445 QAFQITAAEAQQLKQTRSQHT 453
            AFQI+  +A+++K    Q T
Sbjct: 434 NAFQISREDARKIKFNNQQTT 443

BLAST of PI0028294 vs. ExPASy Swiss-Prot
Match: A0A1L6K371 (11S globulin OS=Juglans nigra OX=16719 PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.9e-87
Identity = 190/508 (37.40%), Postives = 279/508 (54.92%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MA  ++L I LCL A   +V+   A      +    +C+L R+ A  PS R+E+E G+ E
Sbjct: 1   MAKPILLSISLCLVA---LVNGCLAQSGGRQQPRFGECKLKRLVALEPSNRIEAEAGVIE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
            WD  N++FQCAGVA  R  I PN L LP++ ++P L YI +G G  G+ FPGC  ET+E
Sbjct: 61  SWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLLYIVKGRGITGVLFPGC-PETFE 120

Query: 121 AQSAQLSRSSRRIR-VDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMD 180
                 SR    +R    + D+HQK+R  R+GD+I  PAG   WCYND    +V VA MD
Sbjct: 121 ESQQGQSRIRPSLRSASFQRDRHQKIRHFREGDVIAFPAGVAHWCYNDGDTPVVAVALMD 180

Query: 181 LNNDDNQLDLRVRSSYLAG---------------------------GVPREARRVSKSDD 240
             N+ NQLD   R+ YLAG                           G P + +R S +  
Sbjct: 181 TTNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRHGEPGQQQRGSGN-- 240

Query: 241 FVNIFSGFDKEFLEEAYNIPSDLARKMQEERS-AGLIVKCD-EEMSFMTP-----EEEEE 300
             N+FSGFD +FL +A+N+ ++ AR++Q E      IV+ +  ++  + P     E+E E
Sbjct: 241 --NVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQERE 300

Query: 301 ELSGSPFSRREE-----------DLNGLEETICTARVQHNMNTQREADAYSREAGRVNIL 360
           E       R  E           D NGLEETICT R++ N+     AD Y+ EAGR++  
Sbjct: 301 ERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRISTA 360

Query: 361 NQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAEIEIANDYGNQVFKE 420
           N   LP+LR++ +SAE+G L+ +A Y  HW++  H + Y + G AE+++ +++G  VF +
Sbjct: 361 NSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFDD 420

Query: 421 KVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTSFFRALPLQLLEQAF 463
           ++  G +  IPQ +    RA  +GFEW++FKT+   M SP+AG TS  RALP ++L  A 
Sbjct: 421 ELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLANAL 480

BLAST of PI0028294 vs. ExPASy Swiss-Prot
Match: Q2TPW5 (11S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.9e-87
Identity = 180/489 (36.81%), Postives = 276/489 (56.44%), Query Frame = 0

Query: 9   ILLCLFAFESVVSTHHAPERHWFREEAQ--QCRLDRIQARPPSRRVESEGGITEVWDEAN 68
           ILL ++ F  V   +    +   R++ Q  QC+L+R+ A  P+ R+E+E G+ E WD  N
Sbjct: 5   ILLSIYLFLIVALFNGCLAQSGGRQQQQFGQCQLNRLDALEPTNRIEAEAGVIESWDPNN 64

Query: 69  EEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYEAQSAQL 128
           ++FQCAGVA  R  I PN L LP++ ++P L YI +G G  G+ FPGC  ET+E    Q 
Sbjct: 65  QQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLVYIARGRGITGVLFPGC-PETFEESQRQS 124

Query: 129 SRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMDLNNDDNQ 188
            +   R   + ++D+HQK+R  R+GD+I  PAG   W YND    +V ++ +D NN+ NQ
Sbjct: 125 QQGQSR---EFQQDRHQKIRHFREGDIIAFPAGVAHWSYNDGSNPVVAISLLDTNNNANQ 184

Query: 189 LDLRVRSSYLAGGVP---------------REARRVSKSDDF--------VNIFSGFDKE 248
           LD   R+ YLAG                  R+ +R  +  +          N+FSGFD +
Sbjct: 185 LDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQRQQRPGEHGQQQRGLGNNVFSGFDAD 244

Query: 249 FLEEAYNIPSDLARKMQEERS-AGLIVKCD-EEMSFMTP-----EEEEEELSGSPFSRRE 308
           FL +A+N+ ++ AR++Q E      IV+ +  ++  + P     E+E EE       R  
Sbjct: 245 FLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQEREERKERERERES 304

Query: 309 E-----------DLNGLEETICTARVQHNMNTQREADAYSREAGRVNILNQLKLPILRFM 368
           E           D NGLEETICT R++ N+     AD Y+ EAGR++ +N   LP+LR++
Sbjct: 305 ESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRISTVNSHTLPVLRWL 364

Query: 369 GMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIP 428
            +SAE+G L+ +A Y  HW++  H + Y + G AE+++ +++G  VF +++  G +  IP
Sbjct: 365 QLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFDDELREGQLLTIP 424

Query: 429 QFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTSFFRALPLQLLEQAFQITAAEAQQLK 455
           Q +    RA  +GFEW++FKT+   M SP+AG TS  RALP ++L  AFQI   +A++LK
Sbjct: 425 QNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLATAFQIPREDARRLK 484

BLAST of PI0028294 vs. ExPASy TrEMBL
Match: A0A5A7T3J7 (11S globulin seed storage protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G001280 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.2e-255
Identity = 444/465 (95.48%), Postives = 457/465 (98.28%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MATKVVLGILL LFAFESVVSTHHAPERH FREEAQQCRLDRIQARPPSRR+ESEGGITE
Sbjct: 1   MATKVVLGILLGLFAFESVVSTHHAPERHRFREEAQQCRLDRIQARPPSRRIESEGGITE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAY+EQGEGFLGLNFPGCNVETYE
Sbjct: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYVEQGEGFLGLNFPGCNVETYE 120

Query: 121 AQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMDL 180
           AQSAQL RSSRRIR+DKEEDKHQKVRRVR+GDMIVIPAGTVQWCYNDCGQDLVVVAF+DL
Sbjct: 121 AQSAQLPRSSRRIRLDKEEDKHQKVRRVRRGDMIVIPAGTVQWCYNDCGQDLVVVAFLDL 180

Query: 181 NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDFVNIFSGFDKEFLEEAYNIPSDLARKMQ 240
           NNDDNQLDLRVRSSYLAGGVPREARRVSKSDD VNIFSGF+KEFLEEAYNIPSDLA+KMQ
Sbjct: 181 NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDLVNIFSGFNKEFLEEAYNIPSDLAKKMQ 240

Query: 241 EERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQHNMNTQRE 300
           EERS GLIVKCDEEMSFMTPEE EEELS SPFSRREED NGLEETICTA+VQHNMNTQRE
Sbjct: 241 EERSRGLIVKCDEEMSFMTPEEGEEELSTSPFSRREEDSNGLEETICTAKVQHNMNTQRE 300

Query: 301 ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAE 360
           ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMT+HRLAYVIEGEAE
Sbjct: 301 ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTNHRLAYVIEGEAE 360

Query: 361 IEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTS 420
           IEIANDYGNQVFK+KVSRGSMF+IPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAG+TS
Sbjct: 361 IEIANDYGNQVFKKKVSRGSMFVIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGYTS 420

Query: 421 FFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTSTRRFS 466
           FFRALPLQLLEQAFQITAAEAQQLKQTR+QHTFLFPPSTSTRRFS
Sbjct: 421 FFRALPLQLLEQAFQITAAEAQQLKQTRAQHTFLFPPSTSTRRFS 465

BLAST of PI0028294 vs. ExPASy TrEMBL
Match: A0A6J1HV45 (11S globulin seed storage protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111467114 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 6.9e-209
Identity = 372/473 (78.65%), Postives = 416/473 (87.95%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MAT+VVL ILLCL A  S    H   ERH FREEAQQCRLDRI++ PPSRR+ESEGGITE
Sbjct: 1   MATRVVLAILLCLVACGSSSQLH---ERHSFREEAQQCRLDRIRSMPPSRRIESEGGITE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           +WDEA E+FQCAGVAA RNIIRPN LSLPKFHSSPML YIEQGEGFLGLN+PGC  ETYE
Sbjct: 61  LWDEAEEDFQCAGVAAVRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNYPGC-AETYE 120

Query: 121 AQSAQLSRSS-----RRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVV 180
           AQSAQ SR S     RRI   KE+D+HQKVRRVR+GDMIV+PAGTVQWC+ND GQDL+ +
Sbjct: 121 AQSAQSSRRSSRRMGRRIGAGKEDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLIAI 180

Query: 181 AFMDLNNDDNQLDLRVRSSYLAGGVPREARRV---SKSDDFVNIFSGFDKEFLEEAYNIP 240
           AF+DLNN+DNQLDLR+R S+LAGG+PRE RR    SK++D VNIFSGFD+EFL +AYN+P
Sbjct: 181 AFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIFSGFDQEFLAQAYNVP 240

Query: 241 SDLARKMQEERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQ 300
           +DL RKMQEERS+GLIVKCDE+MSF+TPEEEEEELS SP SRREE  NGLEETICTARVQ
Sbjct: 241 TDLVRKMQEERSSGLIVKCDEQMSFLTPEEEEEELSESP-SRREEISNGLEETICTARVQ 300

Query: 301 HNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLA 360
           HNMNTQREAD YSREAGRVNILNQLKLPILRFMGMSAEKGHLF NAQYNLHWSMTDHRL 
Sbjct: 301 HNMNTQREADVYSREAGRVNILNQLKLPILRFMGMSAEKGHLFSNAQYNLHWSMTDHRLV 360

Query: 361 YVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMK 420
           YV++GEA  +I++DYGNQVF E+VSRG+MF+IPQFYP+  +AG +GFEWITFKTSNQPMK
Sbjct: 361 YVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQPMK 420

Query: 421 SPVAGHTSFFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPS--TSTRR 464
           SP+AG+TSFFRALPLQLLEQ+FQIT AEAQQLKQTR QHTFLFPPS  +S+RR
Sbjct: 421 SPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGSSSRR 468

BLAST of PI0028294 vs. ExPASy TrEMBL
Match: A0A6J1HGI1 (11S globulin seed storage protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464084 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 2.9e-207
Identity = 369/474 (77.85%), Postives = 415/474 (87.55%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           M T+VVL ILLCL A  S    H   ER  FREEAQQCRLDRI++ PPSRR+ESEGGITE
Sbjct: 1   MPTRVVLAILLCLVACGSSSQLH---ERRSFREEAQQCRLDRIRSLPPSRRIESEGGITE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           +WDEA E+FQCAGVAA RNIIRPN LSLPKFHSSPML YIEQGEGFLGLNFPGC  ETYE
Sbjct: 61  LWDEAEEDFQCAGVAAIRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNFPGC-AETYE 120

Query: 121 AQSAQLSRSS-----RRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVV 180
           AQSAQ SR S     RRI   KE+D+HQKVRRVR+GDMIV+PAGTVQWC+ND GQDLV V
Sbjct: 121 AQSAQSSRRSSRRMGRRIGAGKEDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLVAV 180

Query: 181 AFMDLNNDDNQLDLRVRSSYLAGGVPREARRV---SKSDDFVNIFSGFDKEFLEEAYNIP 240
           AF+DLNN+DNQLDLR+R S+LAGG+PRE RR    SK++D VNI++GFD++FL +AYN+P
Sbjct: 181 AFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIYNGFDQDFLAQAYNVP 240

Query: 241 SDLARKMQEERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQ 300
           +DL R+MQEERS+GLIVKCDE+MSF+TPEEEEEELS SP SRREE  NGLEETICTARVQ
Sbjct: 241 TDLVRRMQEERSSGLIVKCDEQMSFLTPEEEEEELSESP-SRREEISNGLEETICTARVQ 300

Query: 301 HNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLA 360
           HNMNTQREAD YSREAGR+NILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRL 
Sbjct: 301 HNMNTQREADVYSREAGRINILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLV 360

Query: 361 YVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMK 420
           YV++GEA  +I++DYGNQVF E+VSRG+MF+IPQFYP+  +AG +GFEWITFKTSNQPMK
Sbjct: 361 YVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQPMK 420

Query: 421 SPVAGHTSFFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPS---TSTRR 464
           SP+AG+TSFFRALPLQLLEQ+FQIT AEAQQLKQTR QHTFLFPPS   +S+RR
Sbjct: 421 SPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGGSSSRR 469

BLAST of PI0028294 vs. ExPASy TrEMBL
Match: A0A5D3DAK7 (11S globulin seed storage protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G001310 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.1e-205
Identity = 359/471 (76.22%), Postives = 414/471 (87.90%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFR--EEAQQCRLDRIQARPPSRRVESEGGI 60
           MA KV+L ILLC FA ES+V+     ER  FR   EAQ C+LDRI+ RPPSRR+ESEGGI
Sbjct: 1   MAVKVLLAILLCFFACESLVNAQDPAERRRFRFTTEAQLCKLDRIRVRPPSRRIESEGGI 60

Query: 61  TEVWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVET 120
           TE+WDEA+EEFQCAGV A RN IRPNSLSLPKFH++PML YIEQGEGF G+N+PGC  ET
Sbjct: 61  TELWDEADEEFQCAGVGAIRNTIRPNSLSLPKFHNAPMLVYIEQGEGFFGMNYPGC-AET 120

Query: 121 YEAQSAQLSRSSRRI--RVD---KEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLV 180
           YE+QSAQ SRS+RR+  R+     EED+HQK+RRVR+GDMIVIPAGTVQWCYND G+DL+
Sbjct: 121 YESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLI 180

Query: 181 VVAFMDLNNDDNQLDLRVRSSYLAGGVPREARRV---SKSDDFVNIFSGFDKEFLEEAYN 240
            VAF+DLNNDDNQLDLRVR S+LAGGVP EARR    SKSD+ VNIF+G D+EFL EA+N
Sbjct: 181 AVAFLDLNNDDNQLDLRVRGSFLAGGVPSEARREIRGSKSDNLVNIFNGLDQEFLSEAFN 240

Query: 241 IPSDLARKMQEERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTAR 300
           IPSDL R+MQEERS+GLIVKCDEEMSF+TPEEEEEELS + +SRR  + NG+EETICTAR
Sbjct: 241 IPSDLVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSYSRRRGEDNGIEETICTAR 300

Query: 301 VQHNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHR 360
           VQHNMNTQREAD +SREAGRVNILNQLKLPILRF+GMSAEKGHLFPNAQ+NLHWSMTDHR
Sbjct: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFPNAQHNLHWSMTDHR 360

Query: 361 LAYVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQP 420
           + YV++GEAEI+I++DYGNQ+F E+VSRG+MF+IPQFYP+ ARAG +GFEW+TFKTSNQP
Sbjct: 361 MVYVVDGEAEIQISDDYGNQIFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420

Query: 421 MKSPVAGHTSFFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTST 462
           MKSPVAG+TSFFRALPLQLLEQ+FQIT AEAQQLKQTR QHT LFPP++ +
Sbjct: 421 MKSPVAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTLLFPPTSGS 470

BLAST of PI0028294 vs. ExPASy TrEMBL
Match: A0A1S3BHE4 (11S globulin seed storage protein 2-like OS=Cucumis melo OX=3656 GN=LOC103489874 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.1e-205
Identity = 359/471 (76.22%), Postives = 414/471 (87.90%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFR--EEAQQCRLDRIQARPPSRRVESEGGI 60
           MA KV+L ILLC FA ES+V+     ER  FR   EAQ C+LDRI+ RPPSRR+ESEGGI
Sbjct: 1   MAVKVLLAILLCFFACESLVNAQDPAERRRFRFTTEAQLCKLDRIRVRPPSRRIESEGGI 60

Query: 61  TEVWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVET 120
           TE+WDEA+EEFQCAGV A RN IRPNSLSLPKFH++PML YIEQGEGF G+N+PGC  ET
Sbjct: 61  TELWDEADEEFQCAGVGAIRNTIRPNSLSLPKFHNAPMLVYIEQGEGFFGMNYPGC-AET 120

Query: 121 YEAQSAQLSRSSRRI--RVD---KEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLV 180
           YE+QSAQ SRS+RR+  R+     EED+HQK+RRVR+GDMIVIPAGTVQWCYND G+DL+
Sbjct: 121 YESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLI 180

Query: 181 VVAFMDLNNDDNQLDLRVRSSYLAGGVPREARRV---SKSDDFVNIFSGFDKEFLEEAYN 240
            VAF+DLNNDDNQLDLRVR S+LAGGVP EARR    SKSD+ VNIF+G D+EFL EA+N
Sbjct: 181 AVAFLDLNNDDNQLDLRVRGSFLAGGVPSEARREIRGSKSDNLVNIFNGLDQEFLSEAFN 240

Query: 241 IPSDLARKMQEERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTAR 300
           IPSDL R+MQEERS+GLIVKCDEEMSF+TPEEEEEELS + +SRR  + NG+EETICTAR
Sbjct: 241 IPSDLVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSYSRRRGEDNGIEETICTAR 300

Query: 301 VQHNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHR 360
           VQHNMNTQREAD +SREAGRVNILNQLKLPILRF+GMSAEKGHLFPNAQ+NLHWSMTDHR
Sbjct: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFPNAQHNLHWSMTDHR 360

Query: 361 LAYVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQP 420
           + YV++GEAEI+I++DYGNQ+F E+VSRG+MF+IPQFYP+ ARAG +GFEW+TFKTSNQP
Sbjct: 361 MVYVVDGEAEIQISDDYGNQIFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420

Query: 421 MKSPVAGHTSFFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTST 462
           MKSPVAG+TSFFRALPLQLLEQ+FQIT AEAQQLKQTR QHT LFPP++ +
Sbjct: 421 MKSPVAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTLLFPPTSGS 470

BLAST of PI0028294 vs. NCBI nr
Match: XP_004152047.1 (11S globulin seed storage protein 2 [Cucumis sativus] >KAE8650659.1 hypothetical protein Csa_010809 [Cucumis sativus])

HSP 1 Score: 894.8 bits (2311), Expect = 3.1e-256
Identity = 445/464 (95.91%), Postives = 455/464 (98.06%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQ RLDRIQARPPSRR+E EGGITE
Sbjct: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQYRLDRIQARPPSRRIEWEGGITE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIE+GEGFLGLNFPGCNVE YE
Sbjct: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIERGEGFLGLNFPGCNVEEYE 120

Query: 121 AQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMDL 180
           AQSAQLSRSSRRIRVDKEEDKHQKVRRVR+GDMIVIPAGTVQWCYNDCGQDLVVVAFMDL
Sbjct: 121 AQSAQLSRSSRRIRVDKEEDKHQKVRRVRRGDMIVIPAGTVQWCYNDCGQDLVVVAFMDL 180

Query: 181 NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDFVNIFSGFDKEFLEEAYNIPSDLARKMQ 240
           NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDFVNIF+GF+KEFLEEAYNIPSDLARKMQ
Sbjct: 181 NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDFVNIFNGFNKEFLEEAYNIPSDLARKMQ 240

Query: 241 EERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQHNMNTQRE 300
           EERS GLIVKCDEEMSFMTPEEEEEELS  PFSRREED NGLEETICTARVQHNMNTQRE
Sbjct: 241 EERSGGLIVKCDEEMSFMTPEEEEEELSALPFSRREEDSNGLEETICTARVQHNMNTQRE 300

Query: 301 ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAE 360
           AD YSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAE
Sbjct: 301 ADVYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAE 360

Query: 361 IEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTS 420
           IEIANDYGNQVFKE+VSRGSMF+IPQFYPSFARAGP+GFEWITFKTSNQPMKS VAG+TS
Sbjct: 361 IEIANDYGNQVFKERVSRGSMFVIPQFYPSFARAGPRGFEWITFKTSNQPMKSTVAGYTS 420

Query: 421 FFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTSTRRF 465
           FFRALPLQLLEQ+FQITAAEAQQLKQTRSQHTFLFPPSTSTRRF
Sbjct: 421 FFRALPLQLLEQSFQITAAEAQQLKQTRSQHTFLFPPSTSTRRF 464

BLAST of PI0028294 vs. NCBI nr
Match: KAA0038062.1 (11S globulin seed storage protein 2-like [Cucumis melo var. makuwa] >TYK20541.1 11S globulin seed storage protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 891.0 bits (2301), Expect = 4.5e-255
Identity = 444/465 (95.48%), Postives = 457/465 (98.28%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MATKVVLGILL LFAFESVVSTHHAPERH FREEAQQCRLDRIQARPPSRR+ESEGGITE
Sbjct: 1   MATKVVLGILLGLFAFESVVSTHHAPERHRFREEAQQCRLDRIQARPPSRRIESEGGITE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAY+EQGEGFLGLNFPGCNVETYE
Sbjct: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYVEQGEGFLGLNFPGCNVETYE 120

Query: 121 AQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMDL 180
           AQSAQL RSSRRIR+DKEEDKHQKVRRVR+GDMIVIPAGTVQWCYNDCGQDLVVVAF+DL
Sbjct: 121 AQSAQLPRSSRRIRLDKEEDKHQKVRRVRRGDMIVIPAGTVQWCYNDCGQDLVVVAFLDL 180

Query: 181 NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDFVNIFSGFDKEFLEEAYNIPSDLARKMQ 240
           NNDDNQLDLRVRSSYLAGGVPREARRVSKSDD VNIFSGF+KEFLEEAYNIPSDLA+KMQ
Sbjct: 181 NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDLVNIFSGFNKEFLEEAYNIPSDLAKKMQ 240

Query: 241 EERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQHNMNTQRE 300
           EERS GLIVKCDEEMSFMTPEE EEELS SPFSRREED NGLEETICTA+VQHNMNTQRE
Sbjct: 241 EERSRGLIVKCDEEMSFMTPEEGEEELSTSPFSRREEDSNGLEETICTAKVQHNMNTQRE 300

Query: 301 ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAE 360
           ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMT+HRLAYVIEGEAE
Sbjct: 301 ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTNHRLAYVIEGEAE 360

Query: 361 IEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTS 420
           IEIANDYGNQVFK+KVSRGSMF+IPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAG+TS
Sbjct: 361 IEIANDYGNQVFKKKVSRGSMFVIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGYTS 420

Query: 421 FFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTSTRRFS 466
           FFRALPLQLLEQAFQITAAEAQQLKQTR+QHTFLFPPSTSTRRFS
Sbjct: 421 FFRALPLQLLEQAFQITAAEAQQLKQTRAQHTFLFPPSTSTRRFS 465

BLAST of PI0028294 vs. NCBI nr
Match: XP_038889063.1 (11S globulin seed storage protein 2-like [Benincasa hispida])

HSP 1 Score: 802.7 bits (2072), Expect = 1.6e-228
Identity = 403/462 (87.23%), Postives = 429/462 (92.86%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MATKVV+GILLCL AF+S+VS   APERH FREEAQQCRL+RIQAR PSR +ESEGGIT+
Sbjct: 1   MATKVVVGILLCLLAFKSLVSGVQAPERHRFREEAQQCRLERIQARLPSRWIESEGGITQ 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           VW+EANEEFQCAGVAAFRNIIRPNSLSLPK+HS+PML YIEQGEGFLGL+FPGC  ETYE
Sbjct: 61  VWNEANEEFQCAGVAAFRNIIRPNSLSLPKYHSAPMLVYIEQGEGFLGLDFPGC-TETYE 120

Query: 121 AQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMDL 180
           AQS   SRSSRRIR DKEEDKHQKVR+V +GDMIVIPAGTVQWC+ND GQDLVVVAFMDL
Sbjct: 121 AQSGPFSRSSRRIRGDKEEDKHQKVRKVCRGDMIVIPAGTVQWCHNDGGQDLVVVAFMDL 180

Query: 181 NNDDNQLDLRVRSSYLAGGVPREARRVSKSDDFVNIFSGFDKEFLEEAYNIPSDLARKMQ 240
           NNDDNQLDLRVRSSYLA GVPREARR +K+DD VNIFSGF+ EFL EAYNIP DL +KMQ
Sbjct: 181 NNDDNQLDLRVRSSYLASGVPREARRRAKADDLVNIFSGFNPEFLAEAYNIPLDLVKKMQ 240

Query: 241 EERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQHNMNTQRE 300
           EER  GLIVKCDEEMSFMTPEEEEEELS SPFSRREED NGLEETICTARVQHNMNTQ+E
Sbjct: 241 EERGDGLIVKCDEEMSFMTPEEEEEELSESPFSRREEDSNGLEETICTARVQHNMNTQKE 300

Query: 301 ADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAE 360
           AD YSREAGRVNILNQLKLPILRFMGMSAEKGHLF NA+YNLHWSMTDHRLAYVI+GEAE
Sbjct: 301 ADVYSREAGRVNILNQLKLPILRFMGMSAEKGHLFANAEYNLHWSMTDHRLAYVIDGEAE 360

Query: 361 IEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTS 420
           IEIA+DYGNQVFKE+VSRG+MF+IPQFYPSFARAG QGFEWITFKTSNQPMKSPV G+TS
Sbjct: 361 IEIADDYGNQVFKERVSRGNMFVIPQFYPSFARAGQQGFEWITFKTSNQPMKSPVVGYTS 420

Query: 421 FFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTSTR 463
            FRALPLQLLEQAFQIT AEAQQLKQTR+QHTFLFPPSTSTR
Sbjct: 421 LFRALPLQLLEQAFQITTAEAQQLKQTRNQHTFLFPPSTSTR 461

BLAST of PI0028294 vs. NCBI nr
Match: XP_022967670.1 (11S globulin seed storage protein 2-like [Cucurbita maxima])

HSP 1 Score: 736.5 bits (1900), Expect = 1.4e-208
Identity = 372/473 (78.65%), Postives = 416/473 (87.95%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MAT+VVL ILLCL A  S    H   ERH FREEAQQCRLDRI++ PPSRR+ESEGGITE
Sbjct: 1   MATRVVLAILLCLVACGSSSQLH---ERHSFREEAQQCRLDRIRSMPPSRRIESEGGITE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           +WDEA E+FQCAGVAA RNIIRPN LSLPKFHSSPML YIEQGEGFLGLN+PGC  ETYE
Sbjct: 61  LWDEAEEDFQCAGVAAVRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNYPGC-AETYE 120

Query: 121 AQSAQLSRSS-----RRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVV 180
           AQSAQ SR S     RRI   KE+D+HQKVRRVR+GDMIV+PAGTVQWC+ND GQDL+ +
Sbjct: 121 AQSAQSSRRSSRRMGRRIGAGKEDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLIAI 180

Query: 181 AFMDLNNDDNQLDLRVRSSYLAGGVPREARRV---SKSDDFVNIFSGFDKEFLEEAYNIP 240
           AF+DLNN+DNQLDLR+R S+LAGG+PRE RR    SK++D VNIFSGFD+EFL +AYN+P
Sbjct: 181 AFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIFSGFDQEFLAQAYNVP 240

Query: 241 SDLARKMQEERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQ 300
           +DL RKMQEERS+GLIVKCDE+MSF+TPEEEEEELS SP SRREE  NGLEETICTARVQ
Sbjct: 241 TDLVRKMQEERSSGLIVKCDEQMSFLTPEEEEEELSESP-SRREEISNGLEETICTARVQ 300

Query: 301 HNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLA 360
           HNMNTQREAD YSREAGRVNILNQLKLPILRFMGMSAEKGHLF NAQYNLHWSMTDHRL 
Sbjct: 301 HNMNTQREADVYSREAGRVNILNQLKLPILRFMGMSAEKGHLFSNAQYNLHWSMTDHRLV 360

Query: 361 YVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMK 420
           YV++GEA  +I++DYGNQVF E+VSRG+MF+IPQFYP+  +AG +GFEWITFKTSNQPMK
Sbjct: 361 YVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQPMK 420

Query: 421 SPVAGHTSFFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPS--TSTRR 464
           SP+AG+TSFFRALPLQLLEQ+FQIT AEAQQLKQTR QHTFLFPPS  +S+RR
Sbjct: 421 SPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGSSSRR 468

BLAST of PI0028294 vs. NCBI nr
Match: KAG6571523.1 (hypothetical protein SDJN03_28251, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 731.5 bits (1887), Expect = 4.6e-207
Identity = 366/469 (78.04%), Postives = 413/469 (88.06%), Query Frame = 0

Query: 1   MATKVVLGILLCLFAFESVVSTHHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITE 60
           MAT+VVL ILLCL A  S    +   ER  FREEAQQCRLDRI++ PPSRR+ESEGGITE
Sbjct: 1   MATRVVLAILLCLVACGSSSQLY---ERRSFREEAQQCRLDRIRSMPPSRRIESEGGITE 60

Query: 61  VWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYE 120
           +WDEA E+FQCAGVAA RNIIRPN LSLPKFHSSPML YIEQGEGFLGLNFPGC  ETYE
Sbjct: 61  LWDEAEEDFQCAGVAAIRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNFPGC-AETYE 120

Query: 121 AQSAQLSRSS-----RRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVV 180
           AQSAQ SR S     RRI   KE+D+HQKVRRVR+GDMIV+PAGTVQWC+ND GQDLV V
Sbjct: 121 AQSAQSSRRSSRRMGRRIGAGKEDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLVAV 180

Query: 181 AFMDLNNDDNQLDLRVRSSYLAGGVPREARRV---SKSDDFVNIFSGFDKEFLEEAYNIP 240
           AF+DLNN+DNQLDLR+R S+LAGG+PRE RR    SK++D VNI++GFD++FL +AYN+P
Sbjct: 181 AFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIYNGFDQDFLAQAYNVP 240

Query: 241 SDLARKMQEERSAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQ 300
           +DL R+MQEERS+GLIVKCDE+MSF+TPEEEEEELS SP SRREE  NGLEETICTARVQ
Sbjct: 241 TDLVRRMQEERSSGLIVKCDEQMSFLTPEEEEEELSESP-SRREEISNGLEETICTARVQ 300

Query: 301 HNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLA 360
           HNMNTQREAD YSREAGR+NILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRL 
Sbjct: 301 HNMNTQREADVYSREAGRINILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLV 360

Query: 361 YVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMK 420
           YV++GEA  +I++DYGNQVF E+VSRG+MF+IPQFYP+  +AG +GFEWITFKTSNQPMK
Sbjct: 361 YVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQPMK 420

Query: 421 SPVAGHTSFFRALPLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTST 462
           SP+AG+TSFFRALPLQLLEQ+FQIT AEAQQLKQTR QHTFLFPPS+ +
Sbjct: 421 SPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGS 464

BLAST of PI0028294 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 2.7e-72
Identity = 153/456 (33.55%), Postives = 243/456 (53.29%), Query Frame = 0

Query: 13  LFAFESVVST------HHAPERHWFREEAQQCRLDRIQARPPSRRVESEGGITEVWDEAN 72
           LF+  SVVS       H A  R         C   +I +  P++  + E G  EVWD  +
Sbjct: 5   LFSLLSVVSLSFLLFFHGAEARQREAPFPNACHFSQINSLAPAQATKFEAGQMEVWDHMS 64

Query: 73  EEFQCAGVAAFRNIIRPNSLSLPKFHSSPMLAYIEQGEGFLGLNFPGCNVETYEAQSAQL 132
            E +CAGV   R  ++PNS+ LP F S P LAY+ QGEG +G    GC  ET+       
Sbjct: 65  PELRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGC-PETFAEVEGSS 124

Query: 133 SRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYNDCGQDLVVVAFMDLNNDDNQ 192
            R        + ED HQK+   R+GD+    AG  QW YN    D V+V  +D+ N +NQ
Sbjct: 125 GRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIVIVLDVTNRENQ 184

Query: 193 LDLRVRSSYLAGGVPREARRVSKSDDFVNIFSGFDKEFLEEAYNIPSDLARKMQEER-SA 252
           LD   R   LAG   +E  +        N FSGFD   + EA+ I  + A+++Q ++ + 
Sbjct: 185 LDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIAEAFKINIETAKQLQNQKDNR 244

Query: 253 GLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNGLEETICTARVQHNMNTQREADAYS 312
           G I++ +  + F+ P   E +  G          NG+EET CTA++  N++    +D +S
Sbjct: 245 GNIIRANGPLHFVIPPPREWQQDGI--------ANGIEETYCTAKIHENIDDPERSDHFS 304

Query: 313 REAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAEIEIAN 372
             AGR++ LN L LP+LR + ++A +G+L+        W+   H + YV  G+A+I++ +
Sbjct: 305 TRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTANAHTVLYVTGGQAKIQVVD 364

Query: 373 DYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTSFFRAL 432
           D G  VF E+V +G + +IPQ +     AG  GFEWI+FKT++    + ++G TS+ RA+
Sbjct: 365 DNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQTSYLRAV 424

Query: 433 PLQLLEQAFQITAAEAQQLKQTRSQHTFLFPPSTST 462
           P+ +++ ++ +   EA+++K ++ +      PS+S+
Sbjct: 425 PVDVIKASYGVNEEEAKRIKFSQQETMLSMTPSSSS 451

BLAST of PI0028294 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 9.6e-70
Identity = 153/420 (36.43%), Postives = 228/420 (54.29%), Query Frame = 0

Query: 37  QCRLDRIQARPPSRRVESEGGITEVWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPM 96
           +C+LD++ A  PS  ++SE G  EVWD    + +C+GV+  R II    L LP F ++  
Sbjct: 35  ECQLDQLNALEPSHVLKSEAGRIEVWDHHAPQLRCSGVSFARYIIESKGLYLPSFFNTAK 94

Query: 97  LAYIEQGEGFLGLNFPGCNVETYEAQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVI 156
           L+++ +G G +G   PGC  ET++  S    R   + +  +  D HQKV  +R GD I  
Sbjct: 95  LSFVAKGRGLMGKVIPGC-AETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIAT 154

Query: 157 PAGTVQWCYNDCGQDLVVVAFMDLNNDDNQLDLRVRSSYLAGGVPREA--RRVSKSDDFV 216
             G  QW YND  + LV+V+  DL +  NQLD   R  YLAG  P+     +  +     
Sbjct: 155 TPGVAQWFYNDGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQK 214

Query: 217 NIFSGFDKEFLEEAYNIPSDLARKMQ-EERSAGLIVKCDEEMSFMTP--------EEEEE 276
           NIF+GF  E + +A  I    A+++Q ++ + G IV+       + P        EEEEE
Sbjct: 215 NIFNGFGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEE 274

Query: 277 ELSGSPFSRREEDLNGLEETICTARVQHNMNTQREADAYSREAGRVNILNQLKLPILRFM 336
           E       R     NGLEETIC+AR   N++    AD Y  + G ++ LN   LPILRF+
Sbjct: 275 E------GRHGRHGNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFI 334

Query: 337 GMSAEKGHLFPNAQYNLHWSMTDHRLAYVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIP 396
            +SA +G +  NA     W+   + + YV +GEA+I+I ND GN+VF  +VS+G +  +P
Sbjct: 335 RLSALRGSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVP 394

Query: 397 QFYPSFARAGPQGFEWITFKTSNQPMKSPVAGHTSFFRALPLQLLEQAFQITAAEAQQLK 446
           Q +    RA    F+W+ FKT+     + +AG TS  R LPL+++   FQI+  EA+++K
Sbjct: 395 QGFSVVKRATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVK 447

BLAST of PI0028294 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 255.0 bits (650), Expect = 1.2e-67
Identity = 145/415 (34.94%), Postives = 227/415 (54.70%), Query Frame = 0

Query: 37  QCRLDRIQARPPSRRVESEGGITEVWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPM 96
           +C+LD++ A  PS+ ++SEGG  EVWD    + +C+G A  R +I P  L LP F ++  
Sbjct: 29  ECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGK 88

Query: 97  LAYIEQGEGFLGLNFPGCNVETYEAQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVI 156
           L ++  G G +G   PGC  ET+  +S        + +     D HQKV  +R GD I  
Sbjct: 89  LTFVVHGRGLMGRVIPGC-AETF-MESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIAT 148

Query: 157 PAGTVQWCYNDCGQDLVVVAFMDLNNDDNQLDLRVRSSYLAGGVPR-----EARRVSKSD 216
           P+G  QW YN+  + L++VA  DL ++ NQLD  +R   +AG  P+     + R+  K +
Sbjct: 149 PSGVAQWFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQN 208

Query: 217 DFVNIFSGFDKEFLEEAYNIPSDLARKMQEER-SAGLIVKCDEEMSFMTPEEEEEELSGS 276
              NIF+GF  E L +A+ I  + A+++Q ++ + G IVK +     + P     E    
Sbjct: 209 ---NIFNGFAPEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQ 268

Query: 277 PFSRREEDLNGLEETICTARVQHNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAE 336
           P     E  NGLEET+CT R   N++   +AD Y    G ++ LN   LPILR + +SA 
Sbjct: 269 P----HEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSAL 328

Query: 337 KGHLFPNAQYNLHWSMTDHRLAYVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPS 396
           +G +  NA     W++  +   YV  G+A I++ ND G +VF +++S G + ++PQ +  
Sbjct: 329 RGSIRKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSV 388

Query: 397 FARAGPQGFEWITFKTSNQPMKSPVAGHTSFFRALPLQLLEQAFQITAAEAQQLK 446
              A  + FEWI FKT+     + +AG TS  R LPL+++   +QI+  EA+++K
Sbjct: 389 MKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVITNGYQISPEEAKRVK 434

BLAST of PI0028294 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-58
Identity = 142/464 (30.60%), Postives = 218/464 (46.98%), Query Frame = 0

Query: 37  QCRLDRIQARPPSRRVESEGGITEVWDEANEEFQCAGVAAFRNIIRPNSLSLPKFHSSPM 96
           +C LD +     +  ++SE G  E WD  + + +C GV+  R +I    L LP F +SP 
Sbjct: 36  ECNLDNLDVLQATETIKSEAGQIEYWDHNHPQLRCVGVSVARYVIEQGGLYLPTFFTSPK 95

Query: 97  LAYIEQGEGFLGLNFPGCNVETY----------------------------EAQSAQLSR 156
           ++Y+ QG G  G   PGC  ET+                            + Q  Q  R
Sbjct: 96  ISYVVQGTGISGRVVPGC-AETFMDSQPMQGQQQGQPWQGRQGQQGQPWEGQGQQGQQGR 155

Query: 157 SSRRIRVDKEE-------------------------DKHQKVRRVRQGDMIVIPAGTVQW 216
             +      ++                         D HQKV  VR+GD+     G+  W
Sbjct: 156 QGQPWEGQGQQGQQGRQGQQGQPWEGQGQQGQQGFRDMHQKVEHVRRGDVFANTPGSAHW 215

Query: 217 CYNDCGQDLVVVAFMDLNNDDNQLDLRVRSSYLAGGVPREARRVS-KSDDFVNIFSGFDK 276
            YN   Q LV++A +D+ N  NQLD   R  +LAG   +     S +  +  N++SGFD 
Sbjct: 216 IYNSGEQPLVIIALLDIANYQNQLDRNPRVFHLAGNNQQGGFGGSQQQQEQKNLWSGFDA 275

Query: 277 EFLEEAYNIPSDLARKMQEER-SAGLIVKCDEEMSFMTPEEEEEELSGSPFSRREEDLNG 336
           + + +A  I   LA+++Q ++ S G IV+       + P   +   S      R    NG
Sbjct: 276 QVIAQALKIDVQLAQQLQNQQDSRGNIVRVKGPFQVVRPPLRQPYESEEWRHPRSPQGNG 335

Query: 337 LEETICTARVQHNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLFPNAQYN 396
           LEETIC+ R   N++    AD Y    GRV  +N   LPIL ++ +SA +G L  NA   
Sbjct: 336 LEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSATRGVLQGNAMVL 395

Query: 397 LHWSMTDHRLAYVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAGPQGFEW 446
             ++M  + + Y   G+  I++ ND G  V  ++V +G + +IPQ +    ++    FEW
Sbjct: 396 PKYNMNANEILYCTGGQGRIQVVNDNGQNVLDQQVQKGQLVVIPQGFAYVVQSHGNKFEW 455

BLAST of PI0028294 vs. TAIR 10
Match: AT5G44120.2 (RmlC-like cupins superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.8e-53
Identity = 127/350 (36.29%), Postives = 186/350 (53.14%), Query Frame = 0

Query: 107 LGLNFPGCNVETYEAQSAQLSRSSRRIRVDKEEDKHQKVRRVRQGDMIVIPAGTVQWCYN 166
           +G   PGC  ET++  S    R   + +  +  D HQKV  +R GD I    G  QW YN
Sbjct: 1   MGKVIPGC-AETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWFYN 60

Query: 167 DCGQDLVVVAFMDLNNDDNQLDLRVRSSYLAGGVPREA--RRVSKSDDFVNIFSGFDKEF 226
           D  + LV+V+  DL +  NQLD   R  YLAG  P+     +  +     NIF+GF  E 
Sbjct: 61  DGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQKNIFNGFGPEV 120

Query: 227 LEEAYNIPSDLARKMQ-EERSAGLIVKCDEEMSFMTP--------EEEEEELSGSPFSRR 286
           + +A  I    A+++Q ++ + G IV+       + P        EEEEEE       R 
Sbjct: 121 IAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEE------GRH 180

Query: 287 EEDLNGLEETICTARVQHNMNTQREADAYSREAGRVNILNQLKLPILRFMGMSAEKGHLF 346
               NGLEETIC+AR   N++    AD Y  + G ++ LN   LPILRF+ +SA +G + 
Sbjct: 181 GRHGNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIR 240

Query: 347 PNAQYNLHWSMTDHRLAYVIEGEAEIEIANDYGNQVFKEKVSRGSMFIIPQFYPSFARAG 406
            NA     W+   + + YV +GEA+I+I ND GN+VF  +VS+G +  +PQ +    RA 
Sbjct: 241 QNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVVKRAT 300

Query: 407 PQGFEWITFKTSNQPMKSPVAGHTSFFRALPLQLLEQAFQITAAEAQQLK 446
              F+W+ FKT+     + +AG TS  R LPL+++   FQI+  EA+++K
Sbjct: 301 SNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVK 343