PI0028293 (gene) Melon (PI 482460) v1

BLAST of PI0028293 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 4.3e-250
Identity = 412/554 (74.37%), Postives = 477/554 (86.10%), Query Frame = 0

Query: 20  DALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPNVP 79
           +ALTTTL N+I ALGRGFDVT+D+RLLYCKG PGSRLV++++   RDL LS G ++PNVP
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVP 74

Query: 80  DDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAATK 139
            D+ CS     T+ + VCSFH+MAE FN +S + GNIPLG FNAMFN++GSWQVDAA+TK
Sbjct: 75  ADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTK 134

Query: 140 SLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGR 199
           SLA++GY+IPL+ V L    LVL  +I+RAVP +WDP SLASFIEN+GTHI+TS TIGGR
Sbjct: 135 SLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGR 194

Query: 200 DVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAPLSYKDKDVTVIFRRRGGDD 259
           DVVYIRQHQSSPL   EIE+YV ++ + RF  ++SQS   PL YKDKD+TVIFRRRGGDD
Sbjct: 195 DVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDD 254

Query: 260 LVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFL 319
           L QSHA+WA+TV +APD+INMTFTPIVS+LEGVPG++HL RAI+LYLEYKPPIEDLQYFL
Sbjct: 255 LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFL 314

Query: 320 DFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQ 379
           D+QI RAWAPEQ+NLQRKEP+CSSLQFSLMGPKL+IS DQ+TVGRKPVTGLRL+LEG KQ
Sbjct: 315 DYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQ 374

Query: 380 NRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIE 439
           NRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVST+PIE
Sbjct: 375 NRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIE 434

Query: 440 YTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATP 499
           +TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P   +  
Sbjct: 435 HTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGR 494

Query: 500 LRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDK 559
           L   G S+SS T       E+ S  +GKLAKIVD +EM KGPQD+PGHWLVTGAKLGV+K
Sbjct: 495 LEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEK 554

Query: 560 GRIVLRIKYSLLNY 574
           G+IVLR+KYSLLNY
Sbjct: 555 GKIVLRVKYSLLNY 561

BLAST of PI0028293 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.9e-125
Identity = 256/590 (43.39%), Postives = 358/590 (60.68%), Query Frame = 0

Query: 29  SIGALGRGFDVTADIRLLYCK-GTPGSRLVQLDDAHTR-DLVLSDGVVVPNVPDDVQCSS 88
           +IG++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G+ +PNV   ++C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 89  DTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMIGY 148
             R      +  F QMAE FNQ+  ++G IP G FNAMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 149 YIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 208
           +I L+ VAL  S ++LRE +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 209 HQSSPLSEFEIEHYVKEVGEQRFL---------------GSKSQSTAAPLSYKD------ 268
             SS L   +++  +KEV ++RF+                SK ++    L + D      
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 269 ----KDVTVIFRRRGGDDLVQ-SHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 328
               +D   + +RRGG+D     H +W +TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 329 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL----QRKEPLCSSLQFSLMGPKLYIS 388
           AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C+SLQFS  GPKLY++
Sbjct: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVN 373

Query: 389 PDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEE 448
              + VG++P+TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E 
Sbjct: 374 TTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQES 433

Query: 449 QDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLF 508
            D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L F
Sbjct: 434 HDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLCF 493

Query: 509 SKVPGCT-IRRSVWDHSPS-TPATPLRSD------GASSSSKTRTSEDKKEDS------- 568
           S+V G T ++ S WD +    P + L S        A+     R ++     +       
Sbjct: 494 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 553

Query: 569 -STNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSL 571
             T A KL K VD +EM++GPQ+ PG+W+V+GA+L V+KG+I L++KYSL
Sbjct: 554 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of PI0028293 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.0e-114
Identity = 237/599 (39.57%), Postives = 334/599 (55.76%), Query Frame = 0

Query: 29  SIGALGRGFDVTADIRLLYCKGTP-GSRLVQLDDAHTRDLVLSDGVVVPNVPDDVQCSSD 88
           ++  +G G+D+ +D+R   CK TP GSRLV++D    RDL+   G+VV NV   ++C   
Sbjct: 18  AVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCDKG 77

Query: 89  TRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMIGYY 148
            R      + SF+QM+E FNQ   +SG IP G FN MF FS  W  DA++ K+LA  G++
Sbjct: 78  ERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDGWF 137

Query: 149 IPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQH 208
           I L+ V +    L LR+++KR VP +WD  +LA FIE +GTH++   T+GG+DV++++Q 
Sbjct: 138 ISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVKQM 197

Query: 209 QSSPLSEFEIEHYVKEVGEQRF----LGSKSQ-------------------------STA 268
           + S     EI+  +K  G++RF    + SKS                          S+A
Sbjct: 198 RKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVSSA 257

Query: 269 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 328
             +  K++++  +  RRGG DL QSH +W  TV  AP+VI+M F PI S+L G+PG   L
Sbjct: 258 VVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFL 317

Query: 329 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPE----QTNLQRKEPLCSSLQFSLMGPKLY 388
           + A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +    SLQFSLMGPKLY
Sbjct: 318 SHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLY 377

Query: 389 ISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSHVAIGAPKWHG 448
           ++  ++  G +PVTGLR  LEG K N LAIHLQHL + P  L   H D++  I  P   G
Sbjct: 378 VNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKG 437

Query: 449 PEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLK 508
                  ++ P+KW  FSHV T P++Y  A   D +   IVT A L V   G + VL+L+
Sbjct: 438 -------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLFLR 497

Query: 509 LLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTN--------- 568
           L FS       R+S WD+     +T  R  G  S   TR S     + +T          
Sbjct: 498 LGFSLDASAVTRKSCWDNL----STNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 557

Query: 569 ------------AGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLL 572
                         KL  +VD  E+ +GP++ PG+W+VTGAKL V+ G+I ++ KYSLL
Sbjct: 558 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of PI0028293 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 8.7e-110
Identity = 240/614 (39.09%), Postives = 337/614 (54.89%), Query Frame = 0

Query: 29  SIGALGRGFDVTADIRLLYCKGTPGS----RLVQLDDAHTRDLVLSDGVVVPNVPDDVQC 88
           ++ +LG+GFD+TAD RL YCK   GS    RLV LD    R+L +    V  NV  D+ C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 89  SSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNF-SGSWQVDAAATKSLAM 148
               R      +  F++M+EYFNQ+S ++G IP G+FNA F F SGSW  DAA  KSL +
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 149 IGYYIPLFKVALKSSN-LVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVV 208
               + LF + + + N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++GG+DVV
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 209 YIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQST-----------------AAPLSYKD 268
            +RQ +SS L    + H++ ++G+Q F GS   ST                  A   + D
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 269 KD--------------VTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEG 328
           K               +TVI  +RGGD   +SH++W  TV   PD IN  F PI S+L+ 
Sbjct: 252 KQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKD 311

Query: 329 VPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL-----QRKEPLCSSLQF 388
           VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L             +L  
Sbjct: 312 VPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHI 371

Query: 389 SLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHWDSHVA 448
           + MGPKLY++   +T  + PVTG+R  LEG K NRLAIHLQHL +    + +   D H+ 
Sbjct: 372 NFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI- 431

Query: 449 IGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV-HIVTG 508
                W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V  IVTG
Sbjct: 432 -----WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 491

Query: 509 AQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP----------STPATPLRSDGAS 568
           AQL V   G+K+VL+L+L ++KV    + ++ W H P          S+ + PL S    
Sbjct: 492 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 551

Query: 569 SSSKTRTSEDKKEDSSTNAG--------KLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVD 573
            +   +   +   DS    G        K+ K VDL+++ +GPQ  PGHWLVTG +L +D
Sbjct: 552 HNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLD 611

BLAST of PI0028293 vs. ExPASy TrEMBL
Match: A0A5D3CPE6 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008470 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 564/573 (98.43%), Postives = 568/573 (99.13%), Query Frame = 0

Query: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60
           MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGS 120
           DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFH+MAEYFNQKS ISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAMIGYYIP FKVALKSSNLVLREDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFL SKSQS AAP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS+TRTSEDKKEDSSTNAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           PQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of PI0028293 vs. ExPASy TrEMBL
Match: A0A1S3AZC7 (MACPF domain-containing protein CAD1 OS=Cucumis melo OX=3656 GN=LOC103484421 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 564/573 (98.43%), Postives = 568/573 (99.13%), Query Frame = 0

Query: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60
           MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGS 120
           DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFH+MAEYFNQKS ISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAMIGYYIP FKVALKSSNLVLREDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFL SKSQS AAP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS+TRTSEDKKEDSSTNAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           PQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of PI0028293 vs. ExPASy TrEMBL
Match: A0A5A7U9L9 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G002000 PE=4 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 564/600 (94.00%), Postives = 568/600 (94.67%), Query Frame = 0

Query: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60
           MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGS 120
           DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFH+MAEYFNQKS ISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAMIGYYIP FKVALKSSNLVLREDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFL SKSQS AAP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDK---------------------------DVTVIFRRRGGDDLVQSHAQWAKTVKS 300
           LSYKDK                           DVTVIFRRRGGDDLVQSHAQWAKTVKS
Sbjct: 241 LSYKDKVSRQQASSLFSWTVFSEHFTHDPSFLQDVTVIFRRRGGDDLVQSHAQWAKTVKS 300

Query: 301 APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN 360
           APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN
Sbjct: 301 APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN 360

Query: 361 LQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLP 420
           LQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLP
Sbjct: 361 LQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLP 420

Query: 421 KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI 480
           KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI
Sbjct: 421 KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI 480

Query: 481 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSKTRT 540
           VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSS+TRT
Sbjct: 481 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSRTRT 540

Query: 541 SEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           SEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 SEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 600

BLAST of PI0028293 vs. ExPASy TrEMBL
Match: A0A0A0KKH0 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G520370 PE=4 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 544/555 (98.02%), Postives = 548/555 (98.74%), Query Frame = 0

Query: 19  MDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPNV 78
           MDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGSRLVQLDDAH RDLVLSDGVVVPNV
Sbjct: 1   MDALTTTLRNSIAALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHNRDLVLSDGVVVPNV 60

Query: 79  PDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAAT 138
           PDDVQCSSDTRATENVPVCSFH+MAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAAT
Sbjct: 61  PDDVQCSSDTRATENVPVCSFHKMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAAT 120

Query: 139 KSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGG 198
           KSLAMIGYYIPLFKVALKSSNLVL EDIKRAVPYTWDPVSLASFIENFGTHIITSATIGG
Sbjct: 121 KSLAMIGYYIPLFKVALKSSNLVLCEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGG 180

Query: 199 RDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAPLSYKDKDVTVIFRRRGGD 258
           RDVVYIRQHQSSPLSEFEIEHYV+EVGEQRF  SKS+STAAPLSYKDKDVTVIFRRRGGD
Sbjct: 181 RDVVYIRQHQSSPLSEFEIEHYVEEVGEQRFFDSKSRSTAAPLSYKDKDVTVIFRRRGGD 240

Query: 259 DLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYF 318
           DLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL RAIDLYLEYKPPIEDLQYF
Sbjct: 241 DLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLVRAIDLYLEYKPPIEDLQYF 300

Query: 319 LDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCK 378
           LDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCK
Sbjct: 301 LDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCK 360

Query: 379 QNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPI 438
           QNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPI
Sbjct: 361 QNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPI 420

Query: 439 EYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPAT 498
           EYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPAT
Sbjct: 421 EYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPAT 480

Query: 499 PLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVD 558
           PLRSDGASSSS+TRTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVD
Sbjct: 481 PLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVD 540

Query: 559 KGRIVLRIKYSLLNY 574
           KGRIVLRIKYSLLNY
Sbjct: 541 KGRIVLRIKYSLLNY 555

BLAST of PI0028293 vs. ExPASy TrEMBL
Match: A0A6J1CMV2 (MACPF domain-containing protein CAD1 OS=Momordica charantia OX=3673 GN=LOC111012420 PE=4 SV=1)

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 541/575 (94.09%), Postives = 556/575 (96.70%), Query Frame = 0

Query: 1   MKDPRATYQTAA--ATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQ 60
           MKDP A+  TAA  AT+TS+MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60

Query: 61  LDDAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPL 120
           LDDAHTRDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FH+MAEYFNQKS ISGNIPL
Sbjct: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQVDAAATKSLAM+GY IPLFKV LK+SNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTA 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFL SKSQST 
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240

Query: 241 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 300
            PLSYKDKDVTVIFRRRGGDDLVQSHA+WA TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSSKTRTSEDKKEDSS+NAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575

BLAST of PI0028293 vs. NCBI nr
Match: XP_008439705.1 (PREDICTED: MACPF domain-containing protein CAD1 [Cucumis melo] >TYK13232.1 MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa])

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 564/573 (98.43%), Postives = 568/573 (99.13%), Query Frame = 0

Query: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60
           MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGS 120
           DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFH+MAEYFNQKS ISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAMIGYYIP FKVALKSSNLVLREDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFL SKSQS AAP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS+TRTSEDKKEDSSTNAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           PQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of PI0028293 vs. NCBI nr
Match: XP_011658263.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KAE8647726.1 hypothetical protein Csa_003370 [Cucumis sativus])

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 560/573 (97.73%), Postives = 565/573 (98.60%), Query Frame = 0

Query: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60
           MKDPRATYQT+AAT TSNMDALTTTL NSI ALGRGFDVTADIRLLYCKGTPGSRLVQLD
Sbjct: 1   MKDPRATYQTSAATPTSNMDALTTTLRNSIAALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGS 120
           DAH RDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFH+MAEYFNQKSDISGNIPLGS
Sbjct: 61  DAHNRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSDISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVL EDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLCEDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYV+EVGEQRF  SKS+STAAP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVEEVGEQRFFDSKSRSTAAP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL R
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLVR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSS+TRTSEDKKEDSSTNAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573

BLAST of PI0028293 vs. NCBI nr
Match: KAA0052593.1 (MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa])

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 564/600 (94.00%), Postives = 568/600 (94.67%), Query Frame = 0

Query: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60
           MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD
Sbjct: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60

Query: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGS 120
           DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFH+MAEYFNQKS ISGNIPLGS
Sbjct: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQ+DAAATKSLAMIGYYIP FKVALKSSNLVLREDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFL SKSQS AAP
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAP 240

Query: 241 LSYKDK---------------------------DVTVIFRRRGGDDLVQSHAQWAKTVKS 300
           LSYKDK                           DVTVIFRRRGGDDLVQSHAQWAKTVKS
Sbjct: 241 LSYKDKVSRQQASSLFSWTVFSEHFTHDPSFLQDVTVIFRRRGGDDLVQSHAQWAKTVKS 300

Query: 301 APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN 360
           APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN
Sbjct: 301 APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTN 360

Query: 361 LQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLP 420
           LQRKEPLC SLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLP
Sbjct: 361 LQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLP 420

Query: 421 KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI 480
           KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI
Sbjct: 421 KILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHI 480

Query: 481 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSKTRT 540
           VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSS+TRT
Sbjct: 481 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSRTRT 540

Query: 541 SEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           SEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 SEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 600

BLAST of PI0028293 vs. NCBI nr
Match: XP_038883129.1 (MACPF domain-containing protein CAD1 [Benincasa hispida])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 559/573 (97.56%), Postives = 563/573 (98.25%), Query Frame = 0

Query: 1   MKDPRATYQTAAATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLD 60
           MKDPR T QTAAA ATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLV LD
Sbjct: 1   MKDPRTT-QTAAAIATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVHLD 60

Query: 61  DAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGS 120
           DA TRDLVLSDGVVVPNVPDDVQCSSD+RATENVPVCSFH+MAEYFNQKSDISGNIPLGS
Sbjct: 61  DARTRDLVLSDGVVVPNVPDDVQCSSDSRATENVPVCSFHKMAEYFNQKSDISGNIPLGS 120

Query: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLA 180
           FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALK+SNLVL EDIKRAVPYTWDPVSLA
Sbjct: 121 FNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKNSNLVLCEDIKRAVPYTWDPVSLA 180

Query: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAP 240
           SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFL SKSQSTA+P
Sbjct: 181 SFIENFGTHIITSATIGGRDVVYIRQHQSSPLSELEIEHYVKEVGEQRFLDSKSQSTASP 240

Query: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300
           LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR
Sbjct: 241 LSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 300

Query: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQI 360
           AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPDQI
Sbjct: 301 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQI 360

Query: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420
           TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR
Sbjct: 361 TVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSR 420

Query: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480
           WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP
Sbjct: 421 WFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVP 480

Query: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKG 540
           GCTIRRSVWDHSPSTPATPLRSDGASSSSKTR SEDKKEDSSTNAGKLAKIVDLTEMSKG
Sbjct: 481 GCTIRRSVWDHSPSTPATPLRSDGASSSSKTRISEDKKEDSSTNAGKLAKIVDLTEMSKG 540

Query: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 PQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 572

BLAST of PI0028293 vs. NCBI nr
Match: XP_022142258.1 (MACPF domain-containing protein CAD1 [Momordica charantia])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 541/575 (94.09%), Postives = 556/575 (96.70%), Query Frame = 0

Query: 1   MKDPRATYQTAA--ATATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQ 60
           MKDP A+  TAA  AT+TS+MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60

Query: 61  LDDAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPL 120
           LDDAHTRDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FH+MAEYFNQKS ISGNIPL
Sbjct: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQVDAAATKSLAM+GY IPLFKV LK+SNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTA 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFL SKSQST 
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240

Query: 241 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 300
            PLSYKDKDVTVIFRRRGGDDLVQSHA+WA TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSSKTRTSEDKKEDSS+NAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 574
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575

BLAST of PI0028293 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 865.1 bits (2234), Expect = 3.1e-251
Identity = 412/554 (74.37%), Postives = 477/554 (86.10%), Query Frame = 0

Query: 20  DALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPNVP 79
           +ALTTTL N+I ALGRGFDVT+D+RLLYCKG PGSRLV++++   RDL LS G ++PNVP
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVP 74

Query: 80  DDVQCSSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAATK 139
            D+ CS     T+ + VCSFH+MAE FN +S + GNIPLG FNAMFN++GSWQVDAA+TK
Sbjct: 75  ADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTK 134

Query: 140 SLAMIGYYIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGR 199
           SLA++GY+IPL+ V L    LVL  +I+RAVP +WDP SLASFIEN+GTHI+TS TIGGR
Sbjct: 135 SLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGR 194

Query: 200 DVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQSTAAPLSYKDKDVTVIFRRRGGDD 259
           DVVYIRQHQSSPL   EIE+YV ++ + RF  ++SQS   PL YKDKD+TVIFRRRGGDD
Sbjct: 195 DVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDD 254

Query: 260 LVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFL 319
           L QSHA+WA+TV +APD+INMTFTPIVS+LEGVPG++HL RAI+LYLEYKPPIEDLQYFL
Sbjct: 255 LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFL 314

Query: 320 DFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQ 379
           D+QI RAWAPEQ+NLQRKEP+CSSLQFSLMGPKL+IS DQ+TVGRKPVTGLRL+LEG KQ
Sbjct: 315 DYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQ 374

Query: 380 NRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIE 439
           NRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVST+PIE
Sbjct: 375 NRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIE 434

Query: 440 YTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATP 499
           +TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P   +  
Sbjct: 435 HTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGR 494

Query: 500 LRSDGASSSSKTRTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDK 559
           L   G S+SS T       E+ S  +GKLAKIVD +EM KGPQD+PGHWLVTGAKLGV+K
Sbjct: 495 LEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEK 554

Query: 560 GRIVLRIKYSLLNY 574
           G+IVLR+KYSLLNY
Sbjct: 555 GKIVLRVKYSLLNY 561

BLAST of PI0028293 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 451.1 bits (1159), Expect = 1.4e-126
Identity = 256/590 (43.39%), Postives = 358/590 (60.68%), Query Frame = 0

Query: 29  SIGALGRGFDVTADIRLLYCK-GTPGSRLVQLDDAHTR-DLVLSDGVVVPNVPDDVQCSS 88
           +IG++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G+ +PNV   ++C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 89  DTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMIGY 148
             R      +  F QMAE FNQ+  ++G IP G FNAMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 149 YIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 208
           +I L+ VAL  S ++LRE +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 209 HQSSPLSEFEIEHYVKEVGEQRFL---------------GSKSQSTAAPLSYKD------ 268
             SS L   +++  +KEV ++RF+                SK ++    L + D      
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 269 ----KDVTVIFRRRGGDDLVQ-SHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 328
               +D   + +RRGG+D     H +W +TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 329 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL----QRKEPLCSSLQFSLMGPKLYIS 388
           AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C+SLQFS  GPKLY++
Sbjct: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVN 373

Query: 389 PDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEE 448
              + VG++P+TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E 
Sbjct: 374 TTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQES 433

Query: 449 QDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLF 508
            D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L F
Sbjct: 434 HDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLCF 493

Query: 509 SKVPGCT-IRRSVWDHSPS-TPATPLRSD------GASSSSKTRTSEDKKEDS------- 568
           S+V G T ++ S WD +    P + L S        A+     R ++     +       
Sbjct: 494 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 553

Query: 569 -STNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSL 571
             T A KL K VD +EM++GPQ+ PG+W+V+GA+L V+KG+I L++KYSL
Sbjct: 554 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of PI0028293 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 414.5 bits (1064), Expect = 1.4e-115
Identity = 237/599 (39.57%), Postives = 334/599 (55.76%), Query Frame = 0

Query: 29  SIGALGRGFDVTADIRLLYCKGTP-GSRLVQLDDAHTRDLVLSDGVVVPNVPDDVQCSSD 88
           ++  +G G+D+ +D+R   CK TP GSRLV++D    RDL+   G+VV NV   ++C   
Sbjct: 18  AVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCDKG 77

Query: 89  TRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMIGYY 148
            R      + SF+QM+E FNQ   +SG IP G FN MF FS  W  DA++ K+LA  G++
Sbjct: 78  ERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYDGWF 137

Query: 149 IPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQH 208
           I L+ V +    L LR+++KR VP +WD  +LA FIE +GTH++   T+GG+DV++++Q 
Sbjct: 138 ISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHVKQM 197

Query: 209 QSSPLSEFEIEHYVKEVGEQRF----LGSKSQ-------------------------STA 268
           + S     EI+  +K  G++RF    + SKS                          S+A
Sbjct: 198 RKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPFGTSVSSA 257

Query: 269 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 328
             +  K++++  +  RRGG DL QSH +W  TV  AP+VI+M F PI S+L G+PG   L
Sbjct: 258 VVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFL 317

Query: 329 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPE----QTNLQRKEPLCSSLQFSLMGPKLY 388
           + A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +    SLQFSLMGPKLY
Sbjct: 318 SHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLY 377

Query: 389 ISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSHVAIGAPKWHG 448
           ++  ++  G +PVTGLR  LEG K N LAIHLQHL + P  L   H D++  I  P   G
Sbjct: 378 VNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKG 437

Query: 449 PEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLK 508
                  ++ P+KW  FSHV T P++Y  A   D +   IVT A L V   G + VL+L+
Sbjct: 438 -------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLFLR 497

Query: 509 LLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSKTRTSEDKKEDSSTN--------- 568
           L FS       R+S WD+     +T  R  G  S   TR S     + +T          
Sbjct: 498 LGFSLDASAVTRKSCWDNL----STNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 557

Query: 569 ------------AGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLL 572
                         KL  +VD  E+ +GP++ PG+W+VTGAKL V+ G+I ++ KYSLL
Sbjct: 558 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of PI0028293 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 399.1 bits (1024), Expect = 6.2e-111
Identity = 240/614 (39.09%), Postives = 337/614 (54.89%), Query Frame = 0

Query: 29  SIGALGRGFDVTADIRLLYCKGTPGS----RLVQLDDAHTRDLVLSDGVVVPNVPDDVQC 88
           ++ +LG+GFD+TAD RL YCK   GS    RLV LD    R+L +    V  NV  D+ C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 89  SSDTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNF-SGSWQVDAAATKSLAM 148
               R      +  F++M+EYFNQ+S ++G IP G+FNA F F SGSW  DAA  KSL +
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 149 IGYYIPLFKVALKSSN-LVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVV 208
               + LF + + + N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++GG+DVV
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 209 YIRQHQSSPLSEFEIEHYVKEVGEQRFLGSKSQST-----------------AAPLSYKD 268
            +RQ +SS L    + H++ ++G+Q F GS   ST                  A   + D
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDD 251

Query: 269 KD--------------VTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEG 328
           K               +TVI  +RGGD   +SH++W  TV   PD IN  F PI S+L+ 
Sbjct: 252 KQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKD 311

Query: 329 VPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL-----QRKEPLCSSLQF 388
           VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L             +L  
Sbjct: 312 VPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHI 371

Query: 389 SLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHWDSHVA 448
           + MGPKLY++   +T  + PVTG+R  LEG K NRLAIHLQHL +    + +   D H+ 
Sbjct: 372 NFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI- 431

Query: 449 IGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV-HIVTG 508
                W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V  IVTG
Sbjct: 432 -----WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 491

Query: 509 AQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSP----------STPATPLRSDGAS 568
           AQL V   G+K+VL+L+L ++KV    + ++ W H P          S+ + PL S    
Sbjct: 492 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 551

Query: 569 SSSKTRTSEDKKEDSSTNAG--------KLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVD 573
            +   +   +   DS    G        K+ K VDL+++ +GPQ  PGHWLVTG +L +D
Sbjct: 552 HNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLD 611

BLAST of PI0028293 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 240.0 bits (611), Expect = 4.8e-63
Identity = 129/310 (41.61%), Postives = 185/310 (59.68%), Query Frame = 0

Query: 29  SIGALGRGFDVTADIRLLYCK-GTPGSRLVQLDDAHTR-DLVLSDGVVVPNVPDDVQCSS 88
           +IG++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G+ +PNV   ++C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 89  DTRATENVPVCSFHQMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAATKSLAMIGY 148
             R      +  F QMAE FNQ+  ++G IP G FNAMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 149 YIPLFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 208
           +I L+ VAL  S ++LRE +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 209 HQSSPLSEFEIEHYVKEVGEQRFL---------------GSKSQSTAAPLSYKD------ 268
             SS L   +++  +KEV ++RF+                SK ++    L + D      
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 269 ----KDVTVIFRRRGGDDLVQ-SHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHLAR 311
               +D   + +RRGG+D     H +W +TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313