PI0028276 (gene) Melon (PI 482460) v1

Overview


Name	PI0028276
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
Location	chr12: 24190530 .. 24192050 (-)
RNA-Seq Expression	PI0028276
Synteny	PI0028276

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCGAAATAATTAACACAAATTTGGATGCCAATGGCGAACTCGTCGCTGTCATTTTTGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTTGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTCCATTTCATCTCTTGTGACTTGCTCGACCGCCGGTCCGTTCAACAGAATCTCTCCCTGGTTCGCCACGTCACCCACCTTTTTGGATCACTTGGGCCGCTCAGCTCCGGTTCGATTCCCGGGGTTGCTCCGACCAGAACCGGACCATGTTGGCCAACGCCCTCGACGCCATTCTCCCGTCGGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGATCAAACATTACGCTTCGTTGACGAGACTTGCAGCCGGCTGCGGCAGAGGAGGAGGAGAGGAGGTAGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGCCGGAGAGTGGTGGCGTGGAGTGTTCTCCGGCCCGGTTTGATTTTGGGATGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACAGCGGCGTGCTGGGAGGAGGCTTATATCGACGGTTCCGATGCTCGACTCACGGCGGAGCAGCACATGTGGGTCGCAACTAAAGCTGTTGAAATAAATGGTACGTGACTTCTTCACAGGCCTAGACAATTTGATGAGCTACACGAACAAGTCTAAATACTTTCTTTTCATTTTTGTTAAATGAATTAATATTTCATAAATGTCGTGAATTACCTCAAAATATGGGAAAAAATTTATAAAATGAGTAACGATTGTTACATCTATTTTCATTAGCAATAATTGCACCTATTATCAAACTTTCCCCACATTTACACACTAAGAAAAGATTTAACCGTTGAAAAGCCTGATCAAAACATAACGATCATCAATTCACCGAGAAGTTTAAGTAGATAGATGAGAGGTGAATTTAATATTACATTCTTAAAATACAGCAACCGCAGACGGAGAAGCATTCAACGTGTGCAATGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGACAACGACGGCGGAGGAAGAGAACCAGAGAATGTTTTCGACGGAGTTTCACTATACGGCGGCGATGGGGGACAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTTACGACGGAAATGGAAGAGCTGGCGAATTGGGGGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCCGACCGATTTGGGTTCAATGTGAAATACAAGATGTTGGATTCTATCTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

mRNA sequence

ATGGCGGCCGAAATAATTAACACAAATTTGGATGCCAATGGCGAACTCGTCGCTGTCATTTTTGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTTGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTCCATTTCATCTCTTGTGACTTGCTCGACCGCCGGTCCGTTCAACAGAATCTCTCCCTGGTTCGCCACCTCCGGTTCGATTCCCGGGGTTGCTCCGACCAGAACCGGACCATGTTGGCCAACGCCCTCGACGCCATTCTCCCGTCGGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGATCAAACATTACGCTTCGTTGACGAGACTTGCAGCCGGCTGCGGCAGAGGAGGAGGAGAGGAGGTAGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGCCGGAGAGTGGTGGCGTGGAGTGTTCTCCGGCCCGGTTTGATTTTGGGATGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACAGCGGCGTGCTGGGAGGAGGCTTATATCGACGGTTCCGATGCTCGACTCACGGCGGAGCAGCACATGTGGGTCGCAACTAAAGCTGTTGAAATAAATGACGGAGAAGCATTCAACGTGTGCAATGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGACAACGACGGCGGAGGAAGAGAACCAGAGAATGTTTTCGACGGAGTTTCACTATACGGCGGCGATGGGGGACAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTTACGACGGAAATGGAAGAGCTGGCGAATTGGGGGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCCGACCGATTTGGGTTCAATGTGAAATACAAGATGTTGGATTCTATCTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

Coding sequence (CDS)

ATGGCGGCCGAAATAATTAACACAAATTTGGATGCCAATGGCGAACTCGTCGCTGTCATTTTTGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTTGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTCCATTTCATCTCTTGTGACTTGCTCGACCGCCGGTCCGTTCAACAGAATCTCTCCCTGGTTCGCCACCTCCGGTTCGATTCCCGGGGTTGCTCCGACCAGAACCGGACCATGTTGGCCAACGCCCTCGACGCCATTCTCCCGTCGGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGATCAAACATTACGCTTCGTTGACGAGACTTGCAGCCGGCTGCGGCAGAGGAGGAGGAGAGGAGGTAGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGCCGGAGAGTGGTGGCGTGGAGTGTTCTCCGGCCCGGTTTGATTTTGGGATGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACAGCGGCGTGCTGGGAGGAGGCTTATATCGACGGTTCCGATGCTCGACTCACGGCGGAGCAGCACATGTGGGTCGCAACTAAAGCTGTTGAAATAAATGACGGAGAAGCATTCAACGTGTGCAATGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGACAACGACGGCGGAGGAAGAGAACCAGAGAATGTTTTCGACGGAGTTTCACTATACGGCGGCGATGGGGGACAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTTACGACGGAAATGGAAGAGCTGGCGAATTGGGGGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCCGACCGATTTGGGTTCAATGTGAAATACAAGATGTTGGATTCTATCTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

Protein sequence

MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRSVQQNLSLVRHLRFDSRGCSDQNRTMLANALDAILPSAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS

Homology

[Close]

BLAST of PI0028276 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 4.4e-57
Identity = 151/383 (39.43%), Postives = 197/383 (51.44%), Query Frame = 0


Query: 13  NGELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDL 72
           N E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPSWNEDHP-ITYISCDV 83

Query: 73  LDRRSVQQNLSL---VRHL-------RFDSRGCSDQNRTMLANALDAILPSAPALRHVSL 132
           LD   V+  LS    V H+       R   +   + N  ML N L+A++P+ P L+H+ L
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANGKMLKNVLNAMIPNCPNLKHICL 143

Query: 133 QTGIKHY-ASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVV 192
           QTG KHY  +   L + C      +    ED PR +S  N+YY  ED+L E +   +  +
Sbjct: 144 QTGRKHYLGAFEDLKSKC-----HDPPLTEDLPRLDS-QNYYYTQEDILFEEVQ-KKEGL 203

Query: 193 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARL 252
            WSV RPG I G S  +  N +G+LCVY AICK       F G    W + Y D SDA L
Sbjct: 204 TWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAW-DGYSDCSDADL 263

Query: 253 TAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTE 312
            AE  +W A      N  EAFNV NG  F+WK  W  +AE+ GV     EE  Q      
Sbjct: 264 IAEHQIWAAVDPYAKN--EAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEEGQQVKLQD- 323

Query: 313 FHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFN 372
                 M DKG VW +IV E GL TT++E++ NW F D +    E  + +  KS   GF 
Sbjct: 324 -----LMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFL 383

Query: 373 VKYKMLDSILYWIDVMRNDKLIP 381
                 +S + WID ++  K++P
Sbjct: 384 GFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of PI0028276 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.2e-56
Identity = 150/378 (39.68%), Postives = 191/378 (50.53%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKQLAKTL--LSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLDRR 76
           VA+I GVTG+VG  LA+ L    T G  WKVYGVARRP       HP   +I CD+ D  
Sbjct: 28  VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRPRPAWNADHP-VEYIQCDISDAS 87

Query: 77  SVQQNLSL---VRHL-------RFDSRGCSDQNRTMLANALDAILPSAPALRHVSLQTGI 136
                LS    V H+       R     C + N TML N L+A++P A  L H+ LQTG 
Sbjct: 88  DTHTKLSPLTDVTHIFWVTWANRPTESECCELNGTMLRNVLNALIPKAANLHHICLQTGH 147

Query: 137 KHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVAWSVL 196
           KHY          G+    E  + ED PR  +  NFYY LED+L E  S  +  + WSV 
Sbjct: 148 KHYIGPFE---AFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVE-ASEKKAGLNWSVH 207

Query: 197 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLTAEQH 256
           RP +I G S  +  N +G+LCVY AICK    P  F GT A W   Y   SDA L AE  
Sbjct: 208 RPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAW-NCYSVASDADLIAEHQ 267

Query: 257 MWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTEFHYTA 316
           +W A      N  EAFN  NG  F+WK +W  +AE+ GV     +E  +     E     
Sbjct: 268 IWAAVDPYAKN--EAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDESEKPTSLVE----- 327

Query: 317 AMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNVKYKM 376
            M DKG VW EIV E GL TT++E +A W F D +    E ++ S  KS   G+      
Sbjct: 328 RMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILG-GECLLDSMNKSKEHGYLGFRNT 387

Query: 377 LDSILYWIDVMRNDKLIP 381
            +S++  ID M+  K++P
Sbjct: 388 KNSLISVIDKMKAHKIVP 390

[Close]

BLAST of PI0028276 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-55
Identity = 149/382 (39.01%), Postives = 193/382 (50.52%), Query Frame = 0


Query: 13  NGELVAVIFGVTGLVGKQLAKTL----LSTAGWKVYGVARRPDNVSPISHPKFHFISCDL 72
           N E VA+I GVTG+VG  LA+ L         WKVYGVARRP       HP   +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYGVARRPRPSWNEDHP-ITYISCDV 83

Query: 73  LDRRSVQQNLSL---VRHL-------RFDSRGCSDQNRTMLANALDAILPSAPALRHVSL 132
           LD   V+  LS    V H+       R   +   + N  ML N L+A++P+ P L+H+ L
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANGKMLKNVLNAMIPNCPNLKHICL 143

Query: 133 QTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVA 192
           QTG KHY      A    +    +    ED PR +S  NFYY  ED+L E +   +  + 
Sbjct: 144 QTGRKHYLG----AFEDWKIKRHDPPLTEDLPRLDS-QNFYYTQEDILFEEVQ-KKESLT 203

Query: 193 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLT 252
           WSV RPG I G S  +  N +G+LCVY AICK       F G    W + Y D SDA L 
Sbjct: 204 WSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAW-DGYSDCSDADLI 263

Query: 253 AEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTEF 312
           AE  +W A      N  EAFNV NG  F+WK  W  +AE+ GV     EE        E 
Sbjct: 264 AEHQIWAAVDPYAKN--EAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEE------GKEV 323

Query: 313 HYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNV 372
                M DKG VW +IV E GL TT++E++ NW F D +    E  + +  KS   GF  
Sbjct: 324 KLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLG 383

Query: 373 KYKMLDSILYWIDVMRNDKLIP 381
                +S + WID ++  K++P
Sbjct: 384 FRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of PI0028276 vs. ExPASy Swiss-Prot
Match: A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.4e-55
Identity = 147/382 (38.48%), Postives = 199/382 (52.09%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           + VA+I GVTGLVG  LA+   L  T G  WKVYGVARRP       HP  ++ISCD+ +
Sbjct: 27  QCVALIVGVTGLVGNSLAEMLPLSDTPGGPWKVYGVARRPRPSWNEDHP-INYISCDVSN 86

Query: 75  RRSVQQN---LSLVRHL---RFDSRGCSDQ----NRTMLANALDAILPSAPALRHVSLQT 134
              V+     LS V H+    + SR   ++    N  ML N LD ++P+ P L+H+ LQT
Sbjct: 87  TAEVEAKLPPLSDVTHIFYATWTSRSSEEENCEANGKMLKNVLDTMIPNCPNLKHICLQT 146

Query: 135 GIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVAWS 194
           G  HY +           G  +    ED PR  + NNFYY  ED+L E +   +  + WS
Sbjct: 147 GRFHYVA---SVVDWKINGSHDTPLTEDLPRLNT-NNFYYTQEDILLEEVK-RKEGLTWS 206

Query: 195 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLTAE 254
           V RPG I G S  +  N +G+LCVY AICK+      F G    W + Y D +DA L AE
Sbjct: 207 VHRPGTIFGFSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAW-DGYSDCADADLIAE 266

Query: 255 QHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAE-EENQRMFSTEFH 314
            ++W A      N  ++FNV NG  F+WK +W  +AE+ GV     E EE Q++      
Sbjct: 267 HYIWAALDPHAKN--QSFNVSNGDVFKWKHLWKVLAEQFGVECGGYEYEEGQQV-----R 326

Query: 315 YTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNVK 374
               M DKG VW +IV E GL  T++E++  W F DT+    E  + S  KS   GF   
Sbjct: 327 LQDVMKDKGPVWDKIVRENGLSNTKLEDVGKWWFSDTIL-WNECRLDSMNKSKEHGFLGF 386

Query: 375 YKMLDSILYWIDVMRNDKLIPS 382
               +  LYWI  ++  K++PS
Sbjct: 387 RNSKNCFLYWIHKLKAYKIVPS 393

[Close]

BLAST of PI0028276 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.1e-55
Identity = 145/382 (37.96%), Postives = 193/382 (50.52%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RRSVQQNLSL---VRHLRF--------DSRGCSDQNRTMLANALDAILPSAPALRHVSLQ 134
               +  LS    V H+ +        +S  C + N +ML N L AI+P AP LRHV LQ
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNRESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQ 145

Query: 135 TGIKHY-ASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVA 194
           TG KHY    T +      G   +  + ED PR +   NFYY  ED+L E +      V 
Sbjct: 146 TGTKHYLGPFTNV-----DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVT 205

Query: 195 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLT 254
           WS+ RP +I G S  +  N +G+LCVY AICK    P +F G+   W E ++  SDA L 
Sbjct: 206 WSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLI 265

Query: 255 AEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTEF 314
           AEQ +W A      N  EAFN  N   F+WK +W  +AE+ G+     EE          
Sbjct: 266 AEQQIWAAVDPYAKN--EAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE------GKNL 325

Query: 315 HYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNV 374
                M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF  
Sbjct: 326 GLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLG 385

Query: 375 KYKMLDSILYWIDVMRNDKLIP 381
                +S + WID  +  K++P
Sbjct: 386 FRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of PI0028276 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.1e-207
Identity = 363/396 (91.67%), Postives = 367/396 (92.68%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA EIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDRRSVQQNLS VRH            LR DS  CSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTGIKHYASLTRLAAGCG GG  EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLSCGRR+VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGAVAEKLGVATTT 300
           YIDGSDARLTAEQH+WVATKAVEIN   DGEAFNVCNGWSFRWKEIWGAVAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AEEENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEE +RMFS EFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           ASRAKSDR GFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

[Close]

BLAST of PI0028276 vs. ExPASy TrEMBL
Match: A0A1S3C5H7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.8e-205
Identity = 361/393 (91.86%), Postives = 366/393 (93.13%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAA+IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDR+SVQQNLS VRH            LR DS GCSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG EEVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLSCGRR+VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEE 300
           YIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWG VAEKLGVA TTAEE
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGTVAEKLGVA-TTAEE 300

Query: 301 ENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASR 360
           E +RMFS EFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMVASR
Sbjct: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360

Query: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 390

[Close]

BLAST of PI0028276 vs. ExPASy TrEMBL
Match: A0A5D3BGL7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001240 PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.6e-203
Identity = 361/396 (91.16%), Postives = 366/396 (92.42%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAA+IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDR+SVQQNLS VRH            LR DS GCSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG EEVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLSCGRR+VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGAVAEKLGVATTT 300
           YIDGSDARLTAEQHMWVATKAVEIN   DGEAFNVCNGWSFRWKEIWG VAEKLGVA TT
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVA-TT 300

Query: 301 AEEENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEE +RMFS EFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]

BLAST of PI0028276 vs. ExPASy TrEMBL
Match: A0A1S3C4Y3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.6e-203
Identity = 361/396 (91.16%), Postives = 366/396 (92.42%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAA+IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDR+SVQQNLS VRH            LR DS GCSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG EEVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLSCGRR+VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGAVAEKLGVATTT 300
           YIDGSDARLTAEQHMWVATKAVEIN   DGEAFNVCNGWSFRWKEIWG VAEKLGVA TT
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVA-TT 300

Query: 301 AEEENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEE +RMFS EFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]

BLAST of PI0028276 vs. ExPASy TrEMBL
Match: A0A6J1I0N6 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111469437 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 9.4e-180
Identity = 324/396 (81.82%), Postives = 339/396 (85.61%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA E +NTNLDANGELVAVIFGVTGLVGK+LAK LLSTAGWKVYGVARRPDN+SPIS P 
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDRRS   NLS VRH            LRFDS+ CSDQN+ MLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SA ALRHVSLQTG+KHYASLT   +     GG EVVY EDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SASALRHVSLQTGVKHYASLTSSTS----SGGGEVVYSEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLS  RRVVAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGAVAEKLGVATTT 300
           YIDGSDARLTAEQH+W AT+AVEIN   D EAFNVCNGWSF WKEIWGAVAEKLGV   T
Sbjct: 241 YIDGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGV---T 300

Query: 301 AEEENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
              E++RMFS EFHYT AM +KG VWAEIVEEEGLV TEM ELANWGFLD+LFRLPEKMV
Sbjct: 301 VGAEDERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           ASRAKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 361 ASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 389

[Close]

BLAST of PI0028276 vs. NCBI nr
Match: XP_004145319.1 (iridoid synthase CYC2 [Cucumis sativus] >KGN65803.1 hypothetical protein Csa_023251 [Cucumis sativus])

HSP 1 Score: 730.3 bits (1884), Expect = 8.4e-207
Identity = 363/396 (91.67%), Postives = 367/396 (92.68%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA EIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDRRSVQQNLS VRH            LR DS  CSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTGIKHYASLTRLAAGCG GG  EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLSCGRR+VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGAVAEKLGVATTT 300
           YIDGSDARLTAEQH+WVATKAVEIN   DGEAFNVCNGWSFRWKEIWGAVAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AEEENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEE +RMFS EFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           ASRAKSDR GFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

[Close]

BLAST of PI0028276 vs. NCBI nr
Match: XP_008457377.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo])

HSP 1 Score: 723.8 bits (1867), Expect = 7.8e-205
Identity = 361/393 (91.86%), Postives = 366/393 (93.13%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAA+IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDR+SVQQNLS VRH            LR DS GCSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG EEVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLSCGRR+VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEE 300
           YIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWG VAEKLGVA TTAEE
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGTVAEKLGVA-TTAEE 300

Query: 301 ENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASR 360
           E +RMFS EFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMVASR
Sbjct: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360

Query: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 390

[Close]

BLAST of PI0028276 vs. NCBI nr
Match: XP_008457376.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo] >KAA0031816.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo var. makuwa] >TYJ97318.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 718.4 bits (1853), Expect = 3.3e-203
Identity = 361/396 (91.16%), Postives = 366/396 (92.42%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAA+IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDR+SVQQNLS VRH            LR DS GCSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGC  GG EEVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGC--GGREEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLSCGRR+VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGAVAEKLGVATTT 300
           YIDGSDARLTAEQHMWVATKAVEIN   DGEAFNVCNGWSFRWKEIWG VAEKLGVA TT
Sbjct: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVA-TT 300

Query: 301 AEEENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360
           AEEE +RMFS EFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]

BLAST of PI0028276 vs. NCBI nr
Match: XP_038895390.1 ((S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida])

HSP 1 Score: 689.5 bits (1778), Expect = 1.6e-194
Identity = 347/397 (87.41%), Postives = 357/397 (89.92%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAAEIINTNL AN ELVAVIFGVTGLVGK+LAKTLLSTAGWKVYGVARRPDN+SPISHP 
Sbjct: 1   MAAEIINTNLVANAELVAVIFGVTGLVGKELAKTLLSTAGWKVYGVARRPDNISPISHPN 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISCDLLDRRS   NLS VRH            LRFDS+GCSDQNR MLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHSNLSPVRHVTHLFWITWAAQLRFDSQGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTGIKHYASLTRLAAGC  GGG EV YCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGC--GGGGEVAYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLS GRRVV WSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEE 
Sbjct: 181 RLSGGRRVVGWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGAVAEKLGVATTT 300
           YIDGSDARLTAEQH+W ATK ++IN   DGEAFNVCNGWSFRWKEIWGAVAEKLGVA   
Sbjct: 241 YIDGSDARLTAEQHVWAATKGIKINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVA--A 300

Query: 301 AEEENQR-MFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKM 360
           AE+E++R MFS EFHYTAAMGDKGK+WAEIVEEEGLV TEMEELANWGFLDTLFRLPEKM
Sbjct: 301 AEKEDERIMFSAEFHYTAAMGDKGKLWAEIVEEEGLVPTEMEELANWGFLDTLFRLPEKM 360

Query: 361 VASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           VASRAKSDRFGF VKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 VASRAKSDRFGFTVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]

BLAST of PI0028276 vs. NCBI nr
Match: XP_023519075.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647.9 bits (1670), Expect = 5.5e-182
Identity = 325/393 (82.70%), Postives = 341/393 (86.77%), Query Frame = 0


Query: 1   MAAEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA E +NTNLDANGELVAVIFGVTGLVGK+LAK LLSTAGWKVYGVARRPDN+SPIS P 
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSVQQNLSLVRH------------LRFDSRGCSDQNRTMLANALDAILP 120
           FHFISC+LLDRRS   NLS +RH            LRFDS+ CSDQN+ MLANALDAILP
Sbjct: 61  FHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SA ALRHVSLQTG+KHYASLT        GGG EVVYCEDSPRAES NNFYYVLEDLLRE
Sbjct: 121 SALALRHVSLQTGVKHYASLTSSTG----GGGGEVVYCEDSPRAESVNNFYYVLEDLLRE 180

Query: 181 RLSCGRRVVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEA 240
           RLS  RRVVAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKL MPFVFGGTAACWEE 
Sbjct: 181 RLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLNMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEE 300
           YIDGSDARLTAEQH+W ATKAVEINDGEAFNVCNGWSF WKEIWGAVAEKLGV   TA  
Sbjct: 241 YIDGSDARLTAEQHVWAATKAVEINDGEAFNVCNGWSFTWKEIWGAVAEKLGV---TAGA 300

Query: 301 ENQRMFSTEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASR 360
           E++RMFS EFHYTAAM +KG VWAEIVEEEGLV T+M ELANWGFLD+LFRLPEKMVASR
Sbjct: 301 EDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSLFRLPEKMVASR 360

Query: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 382
           AKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 361 AKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 386

[Close]

BLAST of PI0028276 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 386.7 bits (992), Expect = 2.1e-107
Identity = 204/380 (53.68%), Postives = 252/380 (66.32%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKQLAKTLL-STAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRS 74
           E VA+IFGVTGLVG+++ KTLL S  GW++YGVAR P+  S      ++FISCDLL+   
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPEINSMTK--MYNFISCDLLNASE 75

Query: 75  VQQNLS----LVRHL---------RFDSRGCSDQNRTMLANALDAILPSAPALRHVSLQT 134
            +Q LS    +V H+           D+  C  QN+TML NALDAILP+A  L+H SLQT
Sbjct: 76  TKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQT 135

Query: 135 GIKHYASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVAWS 194
           G+KHY SL       G  G     Y E+ PR  SG NFYYVLEDLL+E+++  R  V WS
Sbjct: 136 GMKHYVSLVEETMARGE-GSSLYYYSEECPRKSSGKNFYYVLEDLLKEKIT--RSSVVWS 195

Query: 195 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLTAE 254
           V RPGL++G S+RT +NFMGSLCVYGA+CK L +PFVFGGT  CWEE+YIDGSD+ L AE
Sbjct: 196 VQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAE 255

Query: 255 QHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTEFHY 314
           QH++ AT       GEAFN  NG  F WKEIW  + +KLGV     +     MF   F +
Sbjct: 256 QHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGV-----QVNETTMFDEGFWF 315

Query: 315 TAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNVKY 374
              M ++  VW EIV +E LV TE+E+LANW FLD LFR P K++  R K DRFGF  KY
Sbjct: 316 GREMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKY 375

Query: 375 KMLDSILYWIDVMRNDKLIP 381
           + LDS+LYWIDVMR++KLIP
Sbjct: 376 RTLDSVLYWIDVMRDEKLIP 385

[Close]

BLAST of PI0028276 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-56
Identity = 145/382 (37.96%), Postives = 193/382 (50.52%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RRSVQQNLSL---VRHLRF--------DSRGCSDQNRTMLANALDAILPSAPALRHVSLQ 134
               +  LS    V H+ +        +S  C + N +ML N L AI+P AP LRHV LQ
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNRESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQ 145

Query: 135 TGIKHY-ASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVA 194
           TG KHY    T +      G   +  + ED PR +   NFYY  ED+L E +      V 
Sbjct: 146 TGTKHYLGPFTNV-----DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVT 205

Query: 195 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLT 254
           WS+ RP +I G S  +  N +G+LCVY AICK    P +F G+   W E ++  SDA L 
Sbjct: 206 WSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLI 265

Query: 255 AEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTEF 314
           AEQ +W A      N  EAFN  N   F+WK +W  +AE+ G+     EE          
Sbjct: 266 AEQQIWAAVDPYAKN--EAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE------GKNL 325

Query: 315 HYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNV 374
                M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF  
Sbjct: 326 GLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLG 385

Query: 375 KYKMLDSILYWIDVMRNDKLIP 381
                +S + WID  +  K++P
Sbjct: 386 FRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of PI0028276 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-56
Identity = 145/382 (37.96%), Postives = 193/382 (50.52%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKQLAK--TLLSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+   L  T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 25  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 84

Query: 75  RRSVQQNLSL---VRHLRF--------DSRGCSDQNRTMLANALDAILPSAPALRHVSLQ 134
               +  LS    V H+ +        +S  C + N +ML N L AI+P AP LRHV LQ
Sbjct: 85  AEDTRSKLSPLTDVTHVFYVTWTNRESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQ 144

Query: 135 TGIKHY-ASLTRLAAGCGRGGGEEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRVVA 194
           TG KHY    T +      G   +  + ED PR +   NFYY  ED+L E +      V 
Sbjct: 145 TGTKHYLGPFTNV-----DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVT 204

Query: 195 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEAYIDGSDARLT 254
           WS+ RP +I G S  +  N +G+LCVY AICK    P +F G+   W E ++  SDA L 
Sbjct: 205 WSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLI 264

Query: 255 AEQHMWVATKAVEINDGEAFNVCNGWSFRWKEIWGAVAEKLGVATTTAEEENQRMFSTEF 314
           AEQ +W A      N  EAFN  N   F+WK +W  +AE+ G+     EE          
Sbjct: 265 AEQQIWAAVDPYAKN--EAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE------GKNL 324

Query: 315 HYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFNV 374
                M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF  
Sbjct: 325 GLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLG 384

Query: 375 KYKMLDSILYWIDVMRNDKLIP 381
                +S + WID  +  K++P
Sbjct: 385 FRNSNNSFISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:

BLAST of PI0028276 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A221J5W8	4.4e-57	39.43	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ...	[more]
A0A1C9CX66	2.2e-56	39.68	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
A0A221J5X3	1.1e-55	39.01	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
A0A221J5X1	2.4e-55	38.48	(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ...	[more]
Q9STX2	4.1e-55	37.96	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]

BLAST of PI0028276 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVI2	4.1e-207	91.67	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P...	[more]
A0A1S3C5H7	3.8e-205	91.86	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=...	[more]
A0A5D3BGL7	1.6e-203	91.16	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa...	[more]
A0A1S3C4Y3	1.6e-203	91.16	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1I0N6	9.4e-180	81.82	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of PI0028276 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145319.1	8.4e-207	91.67	iridoid synthase CYC2 [Cucumis sativus] >KGN65803.1 hypothetical protein Csa_023...	[more]
XP_008457377.1	7.8e-205	91.86	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo]	[more]
XP_008457376.1	3.3e-203	91.16	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo] >...	[more]
XP_038895390.1	1.6e-194	87.41	(S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida]	[more]
XP_023519075.1	5.5e-182	82.70	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0028276 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58750.1	2.1e-107	53.68	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	2.9e-56	37.96	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	2.9e-56	37.96	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 19..79 e-value: 2.6E-6 score: 27.1
None	No IPR available	GENE3D	3.40.50.720		coord: 11..380 e-value: 1.6E-90 score: 305.8
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 1..380
None	No IPR available	PANTHER	PTHR32487:SF12	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1	coord: 1..380
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 17..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028276.1	PI0028276.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity