PI0028223 (gene) Melon (PI 482460) v1

Overview


Name	PI0028223
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	mediator of RNA polymerase II transcription subunit 27
Location	chr04: 5589668 .. 5593068 (+)
RNA-Seq Expression	PI0028223
Synteny	PI0028223

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGACACATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAACACTCACTCTCCCGCCTCTGCCGACGACGCTCCTCCCAAGCAGGTCGCTCTCGCCATGGACAGACTCGGTCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGCCTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCCTCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGCAGTTGGTATTTTGCTTCACTTTTTTTCTTCTTCTTTTTTTTTTCTTTTAGGGTTACGACTAAGATCACGCTAGATTTTAATTTTTTACTTCTGATTTTGTTAGGAAAGCAGCTTGAAGAATCTGGTGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAATTATTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTGCTTATGCTTGGAAACGTCAACTAGCTGGCCAGGCTGGTGCATCTGCAGTTGACCGAACCAGGTTAGTTTAGACAGCAAAGCTGCCCTGGGGCATGACTACGGAAGAACTACTTGAAATTTGAAGTTTACTCGACATTTCTTCATTTTGCTTAGTGGTAATTTGGACTCGATCGGCTACGTGCCCAGATTGATGATATTAATAAATTCCCAAATAATTCGATCAAGTATTTTGCATGTATGTAGTAGGTAAGTTCCCATCCATTGGCAGATAGTGAAGCATCAGAGGTTATAGCTGCTTTGGGGTTTATTAGGAATATTTAGATTGGCTAGAGAAGAAGGAATTATCGGTTTTTTTGGCCCCCCAATCGTTTATTTTCTAATCTTTATGCGCCACCCCGCTTTCTTCCCAAAGCCCCTTCATTTTCCTCTCTTTGGAAAGTTGGAATTCCAAAGAGTAAAAATTTTTGCCTGACAGGGTTTGCATGACAAAGTTAACACTTTGGATCACGCTCAGACACATTCTTAGTCGCGTCTTGTTCCTCAGTGGCACATCTTGTGCAGAGGAGCTCTGGAGGATGTTAATCATTTGTTTTGGAGTTGCCAATTTGTGCAATTGATTTGGAGCTGTTTTTGGACTCTTTGAGACTTCTGTCGTTTGTTATAGGGCATGCAGCGCCATGATGGAGGTGGTTGTGTTGTTCCCTCCCTTCAAGAGTATAGATACTACAACTGTGTCCCTGAGTTCTAAGGATAAAGAAAAAGTTATTTGGCAACTTTTTCTCCCTAAATCTTGTGGGTACGTTTGGCTTGAAAAAAAAAAGATCTTTGAGGGTTTGGTCGGTCTTGGCATGTTTTTTTGGGAGAATACTAGGTTGTTTAATACTTGCGATGGCCCCTAAGGTCTTTTGTCATTATCAGTTAGGTGTTATTCTTTTGGATTGGAGACCTTATTATTTATTTCTTTTTGTAGGTTTGGCCTGTTCTCGTGTGGGCTGTCTTTATGTATGCCCCTTGTATCTCCTTTCATGTTTCTCAATAAAAGCTCTATGTTTTATAACATTTAAAAAACAAATAATAACAAGAAGAACAACCCAAATTGTAAGATGAATAAATGCCTTGTGGAAATGATGTTTCATAGTTAACTGGTTATCATTAGGTTATGGCCTGTGTCAAACAACATATTTTGTTCACCTACAAAGGATTTTAATATTGAACATATCTTTTAACACAGAGCGATGAAGTTATTTAATGAATTGACCTTGAATATGTTTTTGCTTATACTTTTTCGTAGGACAGTTTCAGTTGTTTTCAAAATTTTAATACGATAGACAAAATCATTTAATTTTTAATCTGACTTCCATAATTTCAGATGAGTGTTTTACTGATGCTTTGCTTGTTAATAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCATCTTGAAGATGAAAATGGGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTCGGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACTTATGAGCGATTGGACTGGTTAAAACAAGCTTCAGCCCTTCCCTCTCAAGCAAATGAGAATTCAATAGGAGCATTGAAAGAGCATAGCTATCATAGTCCTAGGATGAGCCCTGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCTTCGGTCTTTAGAGCCGTAGTATCACTGCATCCAGTTGGTTCTGTTGAACCAGATGCTCTCGCTTTCTTTTCTCCCGATGAGGTACTCTATTTTCTGCATAAGTATTCATTTTCAAAAAATTTTACTCATTATTCTGTTCTACAGACAACAAGAAAATTTTCCAATAAATGGTGTTATTGCCTGATATGAGGTATTTTACCTCCCAGTTGCATGGTCTAAAGATATGCAACCAGTTGATTTATGAAATAGTTGGCGTGAACCCATAGTATTGTGCCATAATTTTTAACGACCTTTATTAAGTAATATTTATATCATTGACACACACATATAATTCAAAACCAAAATTTTGAATATTATTGTATTGTTGTTCATCATTGTATTATAACAGATCTGAGACTGAGCAGATTTAGTGTTTGTTCATTACAAATGCAATTAGACTTTCTTAGTTTATTTTTCTAAAATTATTATCTCTGCTCGAAGCCTCCACCTGGTTCCGATTCATTGGCCTTTTTCCCACTGATTTTCGTTGTATCGTACTTAAGTTGGTAAATGTTGCAGTTTATTTAGAACTTTTTGTGTTTCAACAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCAAATTATCATTTATTCAGACATGTTACGGTACCCATCACTTTGCTCATAATGTTCACTTTCATTCACATTTATGGAGGTTGCTCTCTCTCAGAGATGATAATTTCGTTTTATCTTGATGCAGGAGCATGCTGCCACGGTGCTGCAGCATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGGTAAAACATCACTCATTGAATTTATACCTTCTTTCTCCTTTCCATTTAAAATCCTCGTCAATTAATTTATGGTACTTTCTTGTAGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGGTGCGTGTAAGGGTTTTTAGTGCTTCCACATTTGGTTTTGTATGGATTTGACATTTTAATGGTGTGTTATCTGATCTTGCAGTAAATGTGGACGGCTGCTATCAGTAAACAAACAATCCGATTTATTGTTACCTCCTGCAGTTCGGCCATACAAACAGTTTTGTGCATCAAAAACTCATCAGCTGTGCCCATAA

mRNA sequence

ATGCGACACATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAACACTCACTCTCCCGCCTCTGCCGACGACGCTCCTCCCAAGCAGGTCGCTCTCGCCATGGACAGACTCGGTCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGCCTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCCTCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGCAGTTGGAAAGCAGCTTGAAGAATCTGGTGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAATTATTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTGCTTATGCTTGGAAACGTCAACTAGCTGGCCAGGCTGGTGCATCTGCAGTTGACCGAACCAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCATCTTGAAGATGAAAATGGGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTCGGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACTTATGAGCGATTGGACTGGTTAAAACAAGCTTCAGCCCTTCCCTCTCAAGCAAATGAGAATTCAATAGGAGCATTGAAAGAGCATAGCTATCATAGTCCTAGGATGAGCCCTGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCTTCGGTCTTTAGAGCCGTAGTATCACTGCATCCAGTTGGTTCTGTTGAACCAGATGCTCTCGCTTTCTTTTCTCCCGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCAAATTATCATTTATTCAGACATGTTACGGAGCATGCTGCCACGGTGCTGCAGCATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGTAAATGTGGACGGCTGCTATCAGTAAACAAACAATCCGATTTATTGTTACCTCCTGCAGTTCGGCCATACAAACAGTTTTGTGCATCAAAAACTCATCAGCTGTGCCCATAA

Coding sequence (CDS)

ATGCGACACATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAACACTCACTCTCCCGCCTCTGCCGACGACGCTCCTCCCAAGCAGGTCGCTCTCGCCATGGACAGACTCGGTCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGCCTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCCTCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGCAGTTGGAAAGCAGCTTGAAGAATCTGGTGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAATTATTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTGCTTATGCTTGGAAACGTCAACTAGCTGGCCAGGCTGGTGCATCTGCAGTTGACCGAACCAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCATCTTGAAGATGAAAATGGGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTCGGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACTTATGAGCGATTGGACTGGTTAAAACAAGCTTCAGCCCTTCCCTCTCAAGCAAATGAGAATTCAATAGGAGCATTGAAAGAGCATAGCTATCATAGTCCTAGGATGAGCCCTGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCTTCGGTCTTTAGAGCCGTAGTATCACTGCATCCAGTTGGTTCTGTTGAACCAGATGCTCTCGCTTTCTTTTCTCCCGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCAAATTATCATTTATTCAGACATGTTACGGAGCATGCTGCCACGGTGCTGCAGCATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGTAAATGTGGACGGCTGCTATCAGTAAACAAACAATCCGATTTATTGTTACCTCCTGCAGTTCGGCCATACAAACAGTTTTGTGCATCAAAAACTCATCAGCTGTGCCCATAA

Protein sequence

MRHIQQPSQGQTATVSPNTHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP

Homology

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BLAST of PI0028223 vs. ExPASy Swiss-Prot
Match: Q8RWM3 (Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana OX=3702 GN=MED27 PE=1 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 9.6e-124
Identity = 229/373 (61.39%), Postives = 285/373 (76.41%), Query Frame = 0


Query: 24  SADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDAS 83
           S  DAPPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDAS
Sbjct: 21  SESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPLQLFLREDAS 80

Query: 84  MRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 143
           MRQHL DLR +GK+LEESGVL+ESL SRSN WGLHMPLVCPDGAVVAYAWKRQLAGQAGA
Sbjct: 81  MRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 140

Query: 144 SAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALKKPCFSHGVTDVKQNELIDSRSLS 203
           SAVDRTRLALKAFTDQKRRFFPH++D  G   EP+ KK   SH + +  + E +D ++L 
Sbjct: 141 SAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGREEPVDYKTLP 200

Query: 204 DILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGAT 263
           DI S +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S ++  G L   
Sbjct: 201 DIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSKLRSG-LQTE 260

Query: 264 PADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT 323
             DK  VIEL  PS+FRA+VSL P GSV+PDA+AFFSPDE GS++HARGFS YH+++H+T
Sbjct: 261 VVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVAFFSPDEGGSYLHARGFSVYHVYKHIT 320

Query: 324 EHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAVRP 383
           EHAAT LQ+FLG     ALY LLLWICS++++FSK C+KCGRLL+++K+S L+LPP  R 
Sbjct: 321 EHAATALQYFLGFGTGTALYSLLLWICSFESVFSKPCTKCGRLLAMDKKSALILPPLHRA 380

Query: 384 YKQFCASKTHQLC 397
           Y++   +    +C
Sbjct: 381 YQELPLALNLDVC 390

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BLAST of PI0028223 vs. ExPASy TrEMBL
Match: A0A0A0LGT1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.1e-218
Identity = 385/397 (96.98%), Postives = 388/397 (97.73%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNTHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSP+THSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDEN SNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYHLFRHVTEHAATVLQ+FLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           SKCGR LSVNKQSDLLLPPAVRPYKQFCASK   + P
Sbjct: 361 SKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP 397

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BLAST of PI0028223 vs. ExPASy TrEMBL
Match: A0A1S3B7L6 (mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 GN=LOC103486676 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 1.8e-218
Identity = 383/397 (96.47%), Postives = 388/397 (97.73%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNTHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSPNTHSP+SADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPNTHSPSSADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDEN SNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSC+EKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCVEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHP+GSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPIGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYH+FRHVTEHAATVLQ+FLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHVFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           SKCGR LSVNKQSDLLLPP VRPYKQFCASK     P
Sbjct: 361 SKCGRRLSVNKQSDLLLPPVVRPYKQFCASKNSSAVP 397

[Close]

BLAST of PI0028223 vs. ExPASy TrEMBL
Match: A0A6J1FP59 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=3662 GN=LOC111446014 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.2e-199
Identity = 355/400 (88.75%), Postives = 372/400 (93.00%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNT---HSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP T   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++ENGSN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQ+F+G Q KAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           KACSKCGRLLSVNKQSDLLLPPA+RPYKQFC SK   + P
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSVVP 400

[Close]

BLAST of PI0028223 vs. ExPASy TrEMBL
Match: A0A6J1KZZ9 (mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=3661 GN=LOC111498620 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 6.1e-198
Identity = 353/400 (88.25%), Postives = 369/400 (92.25%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNT---HSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP T   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++ENGSN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQAS LPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASVLPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIG LKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGTLKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQ+F+G Q KAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           KACSKCGRLLSVNKQSDLLLPPA+RPYKQFC SK     P
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSAVP 400

[Close]

BLAST of PI0028223 vs. ExPASy TrEMBL
Match: A0A6J1DC62 (mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX=3673 GN=LOC111019372 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.0e-192
Identity = 343/400 (85.75%), Postives = 367/400 (91.75%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNT---HSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  QQP+QGQTATVSPNT   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAAPHSGGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGL 120
           LEALCVTA PHQS+KPLHLFQKED SMRQHL DLRAVGKQLEESGVLSESLLSRSN WGL
Sbjct: 61  LEALCVTAHPHQSTKPLHLFQKEDDSMRQHLRDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DE+  N EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDDESRLNLEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           ALKKPCFSHGVT++KQ+EL + R+LSDIL+CMEKEVP+LKIFTYERLDWLKQA AL SQA
Sbjct: 181 ALKKPCFSHGVTEIKQDELNEPRTLSDILTCMEKEVPDLKIFTYERLDWLKQAPALSSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           N+NSIGALKEH+YHSPRM PG+L A+  DK  VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NDNSIGALKEHNYHSPRMGPGSLAASSKDKVSVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFS 360
           FF+PDERGS+VHARGFS +HLFRHVTEHAATVLQ+FLG Q KAALYPLLLWICSYQNLFS
Sbjct: 301 FFAPDERGSYVHARGFSVHHLFRHVTEHAATVLQYFLGIQEKAALYPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           KACSKCGRLLSVNKQSDLLLPPA+RPYKQFC S+     P
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSRVLSAVP 400

[Close]

BLAST of PI0028223 vs. NCBI nr
Match: XP_004136512.1 (mediator of RNA polymerase II transcription subunit 27 isoform X1 [Cucumis sativus])

HSP 1 Score: 768.8 bits (1984), Expect = 2.2e-218
Identity = 385/397 (96.98%), Postives = 388/397 (97.73%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNTHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSP+THSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDEN SNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYHLFRHVTEHAATVLQ+FLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           SKCGR LSVNKQSDLLLPPAVRPYKQFCASK   + P
Sbjct: 361 SKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP 397

[Close]

BLAST of PI0028223 vs. NCBI nr
Match: XP_008442914.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 27 [Cucumis melo])

HSP 1 Score: 768.1 bits (1982), Expect = 3.8e-218
Identity = 383/397 (96.47%), Postives = 388/397 (97.73%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNTHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSPNTHSP+SADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPNTHSPSSADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDEN SNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSC+EKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCVEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHP+GSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPIGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYH+FRHVTEHAATVLQ+FLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHVFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           SKCGR LSVNKQSDLLLPP VRPYKQFCASK     P
Sbjct: 361 SKCGRRLSVNKQSDLLLPPVVRPYKQFCASKNSSAVP 397

[Close]

BLAST of PI0028223 vs. NCBI nr
Match: XP_038904949.1 (mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904950.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904951.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_038904952.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida])

HSP 1 Score: 747.7 bits (1929), Expect = 5.3e-212
Identity = 375/400 (93.75%), Postives = 385/400 (96.25%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNT---HSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  QQP+QGQTATVSPNT   HSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQQPAQGQTATVSPNTAPPHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           ALKKPCFSHGVTDVKQNEL DSRSLS+IL+CMEKEVPELKIFTYERLDWLKQASALPSQA
Sbjct: 181 ALKKPCFSHGVTDVKQNELNDSRSLSEILTCMEKEVPELKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIG LKEHSYHSPRMSPG+LGATP DKAGVIEL+IPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGVLKEHSYHSPRMSPGSLGATPVDKAGVIELVIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQ+FLGNQ KAALYPLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFLGNQAKAALYPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           KACSKCG+LLSVNKQSDLLLPPAVRPYKQFCASK     P
Sbjct: 361 KACSKCGQLLSVNKQSDLLLPPAVRPYKQFCASKNSSSVP 400

[Close]

BLAST of PI0028223 vs. NCBI nr
Match: XP_031739120.1 (mediator of RNA polymerase II transcription subunit 27 isoform X2 [Cucumis sativus] >KAE8651114.1 hypothetical protein Csa_001284 [Cucumis sativus])

HSP 1 Score: 713.0 bits (1839), Expect = 1.4e-201
Identity = 363/394 (92.13%), Postives = 369/394 (93.65%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNTHSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60
           MRHIQQPSQGQTATVSP+THSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA
Sbjct: 1   MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEA 60

Query: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMP 120
           LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGLHMP
Sbjct: 61  LCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMP 120

Query: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALK 180
           LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDEN SNNEPALK
Sbjct: 121 LVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALK 180

Query: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240
           KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN
Sbjct: 181 KPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANEN 240

Query: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300
           SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Sbjct: 241 SIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS 300

Query: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKAC 360
           PDERGSHVHARGFSNYHLFRHVTEHAATVLQ+FLGNQPKAALYPLLLWICSYQNLFSKAC
Sbjct: 301 PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKAC 360

Query: 361 SKCGR-LLSVNKQSDLLLPPAVRPYKQFCASKTH 394
           S   + +L + K          RP   FC+S  H
Sbjct: 361 SSAVQTVLCIKKLISCAHNLIERPEAGFCSSFPH 394

[Close]

BLAST of PI0028223 vs. NCBI nr
Match: XP_022940393.1 (mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata])

HSP 1 Score: 703.7 bits (1815), Expect = 8.7e-199
Identity = 355/400 (88.75%), Postives = 372/400 (93.00%), Query Frame = 0


Query: 1   MRHIQQPSQGQTATVSPNT---HSPASADDAPPKQVALAMDRLGHAARLIADIRLGADRL 60
           MR  Q P+QG TATVSP T   HS  SA++APPKQVALAMDRLGHAARLIADIRLGADRL
Sbjct: 1   MRQTQLPAQGLTATVSPTTVLRHSSGSAEEAPPKQVALAMDRLGHAARLIADIRLGADRL 60

Query: 61  LEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGL 120
           LEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSN WGL
Sbjct: 61  LEALCVTAQPHQSTKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGL 120

Query: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEP 180
           HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++ENGSN EP
Sbjct: 121 HMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLDEENGSNMEP 180

Query: 181 ALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQA 240
           A KKPCFSHGVTDVKQ EL DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQASALPSQA
Sbjct: 181 AQKKPCFSHGVTDVKQEELNDSSSLSDILTCMEKEVPDLKIFTYERLDWLKQASALPSQA 240

Query: 241 NENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA 300
           NENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Sbjct: 241 NENSIGALKEHNYHSPRMSPRSLDATSTDKAAVIELIIPSVFRAVVSLHPVGSVEPDAVA 300

Query: 301 FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQHFLGNQPKAALYPLLLWICSYQNLFS 360
           FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQ+F+G Q KAAL+PLLLWICSYQNLFS
Sbjct: 301 FFSPDERGSHVHARGFSNHHLFRHVTEHAATVLQYFIGIQAKAALHPLLLWICSYQNLFS 360

Query: 361 KACSKCGRLLSVNKQSDLLLPPAVRPYKQFCASKTHQLCP 398
           KACSKCGRLLSVNKQSDLLLPPA+RPYKQFC SK   + P
Sbjct: 361 KACSKCGRLLSVNKQSDLLLPPAIRPYKQFCTSKISSVVP 400

[Close]

BLAST of PI0028223 vs. TAIR 10
Match: AT3G09180.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627); Has 112 Blast hits to 112 proteins in 38 species: Archae - 0; Bacteria - 0; Metazoa - 79; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 444.9 bits (1143), Expect = 6.8e-125
Identity = 229/373 (61.39%), Postives = 285/373 (76.41%), Query Frame = 0


Query: 24  SADDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDAS 83
           S  DAPPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDAS
Sbjct: 21  SESDAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVASQPRHTDMPLQLFLREDAS 80

Query: 84  MRQHLLDLRAVGKQLEESGVLSESLLSRSNYWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 143
           MRQHL DLR +GK+LEESGVL+ESL SRSN WGLHMPLVCPDGAVVAYAWKRQLAGQAGA
Sbjct: 81  MRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGA 140

Query: 144 SAVDRTRLALKAFTDQKRRFFPHLEDENGSNNEPALKKPCFSHGVTDVKQNELIDSRSLS 203
           SAVDRTRLALKAFTDQKRRFFPH++D  G   EP+ KK   SH + +  + E +D ++L 
Sbjct: 141 SAVDRTRLALKAFTDQKRRFFPHIDD--GLKMEPSSKKHRASHLLLENGREEPVDYKTLP 200

Query: 204 DILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGAT 263
           DI S +EK VP +K+ TY RL+WLK+A++LP   +++   A K     S ++  G L   
Sbjct: 201 DIQSRLEKLVPSVKVSTYGRLNWLKRANSLPGSGSDDPTEASKPIFQSSSKLRSG-LQTE 260

Query: 264 PADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT 323
             DK  VIEL  PS+FRA+VSL P GSV+PDA+AFFSPDE GS++HARGFS YH+++H+T
Sbjct: 261 VVDKIAVIELSFPSLFRAIVSLSPAGSVDPDAVAFFSPDEGGSYLHARGFSVYHVYKHIT 320

Query: 324 EHAATVLQHFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRLLSVNKQSDLLLPPAVRP 383
           EHAAT LQ+FLG     ALY LLLWICS++++FSK C+KCGRLL+++K+S L+LPP  R 
Sbjct: 321 EHAATALQYFLGFGTGTALYSLLLWICSFESVFSKPCTKCGRLLAMDKKSALILPPLHRA 380

Query: 384 YKQFCASKTHQLC 397
           Y++   +    +C
Sbjct: 381 YQELPLALNLDVC 390

The following BLAST results are available for this feature:

BLAST of PI0028223 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWM3	9.6e-124	61.39	Mediator of RNA polymerase II transcription subunit 27 OS=Arabidopsis thaliana O...	[more]

BLAST of PI0028223 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGT1	1.1e-218	96.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G781560 PE=3 SV=1	[more]
A0A1S3B7L6	1.8e-218	96.47	mediator of RNA polymerase II transcription subunit 27 OS=Cucumis melo OX=3656 G...	[more]
A0A6J1FP59	4.2e-199	88.75	mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita moschata OX=...	[more]
A0A6J1KZZ9	6.1e-198	88.25	mediator of RNA polymerase II transcription subunit 27 OS=Cucurbita maxima OX=36...	[more]
A0A6J1DC62	1.0e-192	85.75	mediator of RNA polymerase II transcription subunit 27 OS=Momordica charantia OX...	[more]

BLAST of PI0028223 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136512.1	2.2e-218	96.98	mediator of RNA polymerase II transcription subunit 27 isoform X1 [Cucumis sativ...	[more]
XP_008442914.1	3.8e-218	96.47	PREDICTED: mediator of RNA polymerase II transcription subunit 27 [Cucumis melo]	[more]
XP_038904949.1	5.3e-212	93.75	mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida] >XP_0...	[more]
XP_031739120.1	1.4e-201	92.13	mediator of RNA polymerase II transcription subunit 27 isoform X2 [Cucumis sativ...	[more]
XP_022940393.1	8.7e-199	88.75	mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata]	[more]

BLAST of PI0028223 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09180.1	6.8e-125	61.39	CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627);...	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021627	Mediator complex, subunit Med27	PFAM	PF11571	Med27	coord: 314..396 e-value: 2.4E-22 score: 79.1
IPR021627	Mediator complex, subunit Med27	PANTHER	PTHR13130	34 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATED	coord: 2..393
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR13130:SF5	BNAC03G36240D PROTEIN	coord: 2..393

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028223.1	PI0028223.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003713	transcription coactivator activity