PI0028074 (gene) Melon (PI 482460) v1

Overview
NamePI0028074
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionthioredoxin-like fold domain-containing protein MRL7L, chloroplastic
Locationchr05: 10891792 .. 10896861 (-)
RNA-Seq ExpressionPI0028074
SyntenyPI0028074
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAGTTAAAAGGTAAATGTTTTGGAGATTTTTGGGGTTTTAGTTTCTGGGTGTGGAGGGGTATCAAATGACTATTTGAGCTTGGTTTTCGATTCATGGTTAGTTTCTCTTATCTTTCTCTCCTCTGAATCAATCAACACTTTTCGCTTCTCCTTTCTTCTTTATACTTATTAGTTCCATTTCTCTGTATTTTGTTATATTATGCTATATTTTTATTCCGAAACAATAGTTGAAGTCCCAATTTATGCATTCTTGTAGAAATGGCGCTGCAACATACACTGGGTTTACCGTCTCTGGTTTTGCCTTCTTCTAATTGCAAATTTGAAGGGGGTGCTTTATTTGTGTCAACCAATTCCCAAACCACCAATCTTCTCTCTTCATGGACCTTGGAGTCTTCTTATCCTTCATCCATCTCTTTCATGAAGTTTCCTTTCCGGGTAATGTCATTCTCTAATCCATATACTTTGATAACTGGGAATCATTCTGAATTCACTCCATGGCAATTTGACAATTGTAGAGTTGTTTTTAGACATGGTGCAACAACATACTTGTAAATAAATCACTTTGAAGGAATGTAAATAAACAATATTTTTAGGAAAAAAAGGGCTTTAACTATAATGTATGCCCAAGTCACCCTCAGTGCTCGTTTCTTGTTACTTGGGGAGACTAACTGTGAAGCGCTTCATGTTATTATATGTAGGGTAGTTGTTTTAGAAGCAGCACTCTTAGGGCCTATAGAACTGCAGGGTCACTCAGGCCTGGTGATGGAAAGAAGAAGAAAGGACAAGCAGGTATGGACTTAGATGAGGAAGATGATTCTTCTTCAGCGAAGCAGCTAGACGTTGAGCTTATGGATGCCGAAGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCAATGTTGAGGAGGAGATAGACAACATGGAAAGGAGAAGAAAGATGCAAAAACTTCTCGCTGACTATCCTCTTGTTGTGGAAGAGGAGGATCTTGATTGGCCTGAGGATGCTGATGGTTGGGGTTTCAACTTGGGTCAATTTTTTGATAAGATCACCATCAAGAATAAGAAAAAAGATGATAAATACGATGATGATAAAGATGACACTGATAATGAAATAGTTTGGCAAGATGACAACTACATTCGTCCAATTAAGGACATAACAATTTCTGAATGGGAGGAGGCTGTGTTCAAAGATATTAGTCCACTAATCATTTTTGTACATAATCGCTACAAAAGGTATGCTCTTTGAAGTCATCAGTATCTTGTGTGGGGAAAATTGTTTTTATGGCTTTGATTGACATTTATCTCTTTATATATATTTTTTATTAATTCCAAACCTATGATCCAATGCAAGGATCTTGTTCGGTATCCACCCTCGCTTCCAAAAACCCAGCAATGTACTGGTAACAGCCCTGCGATTCTTATATTACGATAGACAATTGTCTAGCAAGTCCACAATATTAACAAGAAATCCTCGATAACATAAACAAGATTTTAACAAGCAAGGAGTTAAAAAAGAAACAAAATGCCATCACATTCGAGAGACCTGGAAATACTCTTTAAGCCTCAACTTCACCAAATTCTTCCCCCTAGATAATGAGGCTGAAACCCCTATGGGCCCTATAAAAACCCCCACCTCAAAGTACAATAGTGAAATACAAAGAGCAACAAAACACAAGAAACAGCAACGAACCCACCCCCAAAACACCCTCAACAAATCCTAATACGACCAACTGATAACAAATATCTAAACAAAATTAACAACGATACAAGCAATCGTGCTTAATCGACCAAGAATACAAGCCAAGAAAAGAAAAATCCGGGCAAAATGAGGCTTTTCAATCAAGAGATAACTTGCTGCTGTGAAGCTAGTAAAGACAGAATGACCCCATCAAGCCAATTGCTTAATTATGAGAGATACGCTGATTCCTTTCAAACCAAAACCAATCTGAAGGATCTTTAACAACAGTAGACCAAATCAACCGTGGCTTAGCTTCCAAGGACTTAACAAAGCCTTTGAGAACATCATCACCGAGAGCTAACTCTCCTTCCAATACTAATCTTAGGTCTTAATATCAAATTGATACGAAAAAATTGGCAAACAACCGCGGGGGGGGGGGGGGAGTTCGAGTGTTCTTCAAATAAAACAGAGCATTGAGGATAGCACCGAAACTATCATTTGCACTACTATTTCTTGGTTATCTTCAACCCAAAATAACCTATGACTTCAATCAAACATAATCGAAGAGTACGCCAAGGAGAACGATTAGCCCAAAATTTGAAACACTCTTTAACCAAAACATCATTAATTGGTGGAAGAGTAGTTTTTCCAAGTATCCTACCTCTAACCTCATGAAACTCAACATTGAGGCTGACAAGGAATTTGTAAATACGACCAACTTCTACAGTTTTTCTGTAATGCTTTTGATTTTCTGTAGACCTCCACTTATATGTATCAAAGAGATCAAGGTCTTGCCAAAATTCTTTTTAGGGAGTTAAAGTATTGTGTCATGGAGTTGCCTCCTTGTCGTATAGCACCTAGTTTAAAGATTCAACTTAAACACTTGTGATTGGTTGCGCAAATCAGAATACATTTGAGTAACACTATTCCATAATTCCTTGTTTAAAAAACATGATCACCTAAATGTCGAAGTGTACACTGGTGCTGATTGGGCAGGTTGCACAACTGATAGAAGATTCACTTCTGGTTATTGCTCATTTGTTGGAGGAAATCTAGTTACTTGGCGAAGTAAAGTACAGAGCGTGGTTGCAAGAAATAGTGTAAAAGTAGAATTTAAGGCATTAGCCCATGGTATTTGTGAGGGCATATGAATAAGAAGACTATCGGAGAAATTGAGATTCTCTCAGAGAATACCTATCCCCATTTATTGTGATAACAAGGCAACAATATCAATTGTCCACAATTTAGTCCTTCATGATAAGACGAAACATATTGAAGTTGATAAACACTTCATAAAGGAAAAGATTGTTCAAGGAATAACATGCATCTCTTATCTTCCGACAATATAACAAATCGCAGATGTGTTAACTATAGGTCTTCCAAAGTGGTAATTCAATAACTTAATTGACAAGCTGGCTATGGATGATATCTTCAAACCAACTTGAGGGGGAGTGTTGATTATTTCCTTTATTGTTAATATTTCCATTGTATTTAAGATATATTTGTGTATTTGTTTTTTCCTACTTTGTATTGGGTATTTCTTTTATTTAAGAAAACCCTTTTCTTTCTAAATGAAATAATAGGAAAACACCTTTTTACACAATTTCTATAACTAGGATTCTTACATTTTCATACCTTTCCAGTAATGTGTTCAGTTGTTATTGTTATACTTTCACGTTATTCTCCCATGGCTTCTTCACTAGATTATAATGGAAGTTTTGAAGTAATAGCTACACTTCAGCCACCATTTCGTTAGATTAGATTCTAAAAGGTCTTTTCATAAGGCTTCCTCTAGGAGAGGAGATGCCTTTTCCCCTTGTCATTAGGCTGTTTTGTTGAGGCTTTGGGCCGCTTTGAATGCTGTTCCTTGTTTCTCAATTCTCATTAAAAAAGGTAATAATGGGTCAGTAGAAGTAGCTGGCAGGACTGGCCCTCATTGTGGAAGTAAAGATATCCCCTCTTCTCCATAATTTGAAGATGGTGATTCTGTTGGTGCAATAGTTTTTTTTCTTTTGTATCCACATACTTTGGTGCATTTCCACCTATAGTTTCTTGAGTCCTCCCTCCATACACTATGCAGTTCCACGAGTTGTTCTTCCACTTCTGAGATCTTCTACTCCCCTGAAATGATACTTTCTTCCATGCTCCAAATACTTAATTGCAAGGATCTTACTGATCTAATCGACTAATACCAATTGGTTATTATTTCTCAAACGACACAAATCATTATGTCAGAAAAGACGAAGTACAAACGAGGAGAGTTTGAGAGCGCCTCCTCTCCAAATGCCACCCTATGTTGTTGAACACACTATTGTGATTTGTCATATTTGATTTCTTGGTTTAACTTGTCAGGAATATGGAGGAGCTTTGGATTGTGAACAGTTAACTTTGAATAATGCTTGAACGTCATGGTTGCAGTGGTTTAACAATAACATGAATCATAAGATGATCTTGATCTGTATATGACAGAAATTTGAACATTTTGAGGGTCATGGTTTGTATGTATTTGTCTTCAATTAGGCCAAAGGAAAATGAAAAAGTTCGCGAGGAGCTGGAGAATGCTATACACATAATATGGAATTGCAATCTGCCTTCGCCAAGGGTAAGCCTTTCCTATTTATTGACGGTACTTTACTCATCTCTCTACCTTTACAAATGCTATTTTAAACGTACTGTTTTTAACTGCAGTGTGTTGCTGTTGACGCCGTTGTTGAACGCAACTTGGTCTCTGCTCTTCAAGTGTCTGCCTTTCCAGAGATTATATTCACTAAAGCAGGGAAAATCTTATACCGTGAGAAGGGTAAGTGCAAAACGAAGAGAAAACATTGCGAGTTTTGTTAATGAAACCTATTTCTCAATGTGAGGTGAGGTCTGAGTTTTGGATTGTTTCAGGATTTGTAAGTGCAGATGAGTTGTCCAAAATAATGGCATTCTTCTACTATGGAGCAGCTAAGCCACTATGTTTAAATGACGTTGGGGATTATCAGGAGGCGATCCCATCAGTGTCTTCGTAATTGTACCATAGAAGGATAGGGTGTACTCGTTTAAAAGAAATTTAAAGTGTAAATTATGCTCTTTTAGGTTTGTTGTAGATGAGTGCAATCGGTTTGTTGGGCCAAATATTTTTGGAAAGGCCGAAGAACATCAGTTATACAACTTTATGGACTATTTAAGGGTATTATAGAAAAATATGTGATGTATCATAACTTACGGTGAGATAGCAACAAGAGCAGCTAGTTGTGATTCTTATATTCTTTTTCATTATCTATATTATATACTTTCATTTTTTTAGTTTTCAAATATAACAAAACTGATGAAATATTTACAAATGTAGCATAACTTTATTTTTTATCAATGATAGACCAAATAAACA

mRNA sequence

GGAAAGTTAAAAGGTAAATGTTTTGGAGATTTTTGGGGTTTTAGTTTCTGGGTGTGGAGGGGTATCAAATGACTATTTGAGCTTGGTTTTCGATTCATGAAATGGCGCTGCAACATACACTGGGTTTACCGTCTCTGGTTTTGCCTTCTTCTAATTGCAAATTTGAAGGGGGTGCTTTATTTGTGTCAACCAATTCCCAAACCACCAATCTTCTCTCTTCATGGACCTTGGAGTCTTCTTATCCTTCATCCATCTCTTTCATGAAGTTTCCTTTCCGGGGTAGTTGTTTTAGAAGCAGCACTCTTAGGGCCTATAGAACTGCAGGGTCACTCAGGCCTGGTGATGGAAAGAAGAAGAAAGGACAAGCAGGTATGGACTTAGATGAGGAAGATGATTCTTCTTCAGCGAAGCAGCTAGACGTTGAGCTTATGGATGCCGAAGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCAATGTTGAGGAGGAGATAGACAACATGGAAAGGAGAAGAAAGATGCAAAAACTTCTCGCTGACTATCCTCTTGTTGTGGAAGAGGAGGATCTTGATTGGCCTGAGGATGCTGATGGTTGGGGTTTCAACTTGGGTCAATTTTTTGATAAGATCACCATCAAGAATAAGAAAAAAGATGATAAATACGATGATGATAAAGATGACACTGATAATGAAATAGTTTGGCAAGATGACAACTACATTCGTCCAATTAAGGACATAACAATTTCTGAATGGGAGGAGGCTGTGTTCAAAGATATTAGTCCACTAATCATTTTTGTACATAATCGCTACAAAAGGCCAAAGGAAAATGAAAAAGTTCGCGAGGAGCTGGAGAATGCTATACACATAATATGGAATTGCAATCTGCCTTCGCCAAGGTGTGTTGCTGTTGACGCCGTTGTTGAACGCAACTTGGTCTCTGCTCTTCAAGTGTCTGCCTTTCCAGAGATTATATTCACTAAAGCAGGGAAAATCTTATACCGTGAGAAGGGATTTGTAAGTGCAGATGAGTTGTCCAAAATAATGGCATTCTTCTACTATGGAGCAGCTAAGCCACTATGTTTAAATGACGTTGGGGATTATCAGGAGGCGATCCCATCAGTGTCTTCGTAATTGTACCATAGAAGGATAGGGTGTACTCGTTTAAAAGAAATTTAAAGTGTAAATTATGCTCTTTTAGGTTTGTTGTAGATGAGTGCAATCGGTTTGTTGGGCCAAATATTTTTGGAAAGGCCGAAGAACATCAGTTATACAACTTTATGGACTATTTAAGGGTATTATAGAAAAATATGTGATGTATCATAACTTACGGTGAGATAGCAACAAGAGCAGCTAGTTGTGATTCTTATATTCTTTTTCATTATCTATATTATATACTTTCATTTTTTTAGTTTTCAAATATAACAAAACTGATGAAATATTTACAAATGTAGCATAACTTTATTTTTTATCAATGATAGACCAAATAAACA

Coding sequence (CDS)

ATGGCGCTGCAACATACACTGGGTTTACCGTCTCTGGTTTTGCCTTCTTCTAATTGCAAATTTGAAGGGGGTGCTTTATTTGTGTCAACCAATTCCCAAACCACCAATCTTCTCTCTTCATGGACCTTGGAGTCTTCTTATCCTTCATCCATCTCTTTCATGAAGTTTCCTTTCCGGGGTAGTTGTTTTAGAAGCAGCACTCTTAGGGCCTATAGAACTGCAGGGTCACTCAGGCCTGGTGATGGAAAGAAGAAGAAAGGACAAGCAGGTATGGACTTAGATGAGGAAGATGATTCTTCTTCAGCGAAGCAGCTAGACGTTGAGCTTATGGATGCCGAAGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCAATGTTGAGGAGGAGATAGACAACATGGAAAGGAGAAGAAAGATGCAAAAACTTCTCGCTGACTATCCTCTTGTTGTGGAAGAGGAGGATCTTGATTGGCCTGAGGATGCTGATGGTTGGGGTTTCAACTTGGGTCAATTTTTTGATAAGATCACCATCAAGAATAAGAAAAAAGATGATAAATACGATGATGATAAAGATGACACTGATAATGAAATAGTTTGGCAAGATGACAACTACATTCGTCCAATTAAGGACATAACAATTTCTGAATGGGAGGAGGCTGTGTTCAAAGATATTAGTCCACTAATCATTTTTGTACATAATCGCTACAAAAGGCCAAAGGAAAATGAAAAAGTTCGCGAGGAGCTGGAGAATGCTATACACATAATATGGAATTGCAATCTGCCTTCGCCAAGGTGTGTTGCTGTTGACGCCGTTGTTGAACGCAACTTGGTCTCTGCTCTTCAAGTGTCTGCCTTTCCAGAGATTATATTCACTAAAGCAGGGAAAATCTTATACCGTGAGAAGGGATTTGTAAGTGCAGATGAGTTGTCCAAAATAATGGCATTCTTCTACTATGGAGCAGCTAAGCCACTATGTTTAAATGACGTTGGGGATTATCAGGAGGCGATCCCATCAGTGTCTTCGTAA

Protein sequence

MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRGSCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWREKIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDKITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNRYKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVSS
Homology
BLAST of PI0028074 vs. ExPASy Swiss-Prot
Match: Q9SKB6 (Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7L PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.5e-100
Identity = 192/274 (70.07%), Postives = 218/274 (79.56%), Query Frame = 0

Query: 82  GKKKKGQAGMDLDEEDDSSSAKQ-----LDVELMDAEERREWREKIREVIDMNPNVE--E 141
           GKK   +   D D ED+    K+      +  L D  ERREWR+ IREVID +P++E  E
Sbjct: 72  GKKVWRKKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIREVIDKHPDIEEDE 131

Query: 142 EIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDKITIKNKKK---DDK 201
           EID +E+RRKMQKLLADYPLVV EED +WPEDADGWGF+  QFF+KITIKN+KK   DD 
Sbjct: 132 EIDMVEKRRKMQKLLADYPLVVNEEDPNWPEDADGWGFSFNQFFNKITIKNEKKEEEDDD 191

Query: 202 YDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNRYKRPKENEKVR 261
            D + DD++ EIVWQDDNYIRPIKD+T +EWEEAVFKDISPL++ VHNRYKRPKENEK R
Sbjct: 192 EDSEGDDSEKEIVWQDDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKRPKENEKFR 251

Query: 262 EELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVS 321
           EELE AI +IWNC LPSPRCVAVDAVVE +LVSAL+VS FPEIIFTKAGKILYREKG  +
Sbjct: 252 EELEKAIQVIWNCGLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKAGKILYREKGIRT 311

Query: 322 ADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSV 346
           ADELSKIMAFFYYGAAKP CLN V + QE IP V
Sbjct: 312 ADELSKIMAFFYYGAAKPPCLNGVVNSQEQIPLV 345

BLAST of PI0028074 vs. ExPASy Swiss-Prot
Match: Q0DI48 (Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7L PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.7e-69
Identity = 143/274 (52.19%), Postives = 186/274 (67.88%), Query Frame = 0

Query: 80  GDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWREKIREVIDMNPNVEEEIDNM 139
           G   +  G      D+EDD    +  D      E+RR  R KIRE++D  P   E  D  
Sbjct: 64  GGRARDDGSESESSDDEDDDEPMQMTD------EQRRTLRRKIREMMDRVPETAEITDPA 123

Query: 140 ERRRKMQKLLADYPLVVEEEDLDWPE-DADGWGFNLGQFFDKITIKNKKKDDKYDDDKDD 199
           ER+ KM +LL  Y LVVEEED +WPE D DG GF+LGQFFDKITIK +KK+D  DD++DD
Sbjct: 124 ERKAKMLELLTKYQLVVEEEDPNWPEDDEDGHGFSLGQFFDKITIKAEKKND--DDEEDD 183

Query: 200 -----TDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNRYKRPKENEKVREE 259
                +D EIVW+DDNYI+PI+D+   +W++ VF D  PLI+ VHNRYKRP++NE  R++
Sbjct: 184 AKGNQSDKEIVWEDDNYIKPIRDVKTMDWDDTVFTDFGPLIVLVHNRYKRPQDNENARDQ 243

Query: 260 LENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSAD 319
           L  AI + W  NLPSPRCVAVDA  E +LV AL VS FPE++FT AGKI++R+K   SA+
Sbjct: 244 LVKAIEMFWEYNLPSPRCVAVDACAEPDLVKALNVSGFPEVLFTNAGKIVHRDKVVRSAE 303

Query: 320 ELSKIMAFFYYGAAKPLCLNDV-GDYQEAIPSVS 347
           E +++MAFFYY AA+P CL++  G  QE +P +S
Sbjct: 304 EWTRMMAFFYYKAARPPCLSEADGQGQEKVPLMS 329

BLAST of PI0028074 vs. ExPASy Swiss-Prot
Match: Q5QLS7 (Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-31
Identity = 88/221 (39.82%), Postives = 122/221 (55.20%), Query Frame = 0

Query: 119 REKIREVIDMNPNV---EEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDAD-GWGFNL 178
           RE+  EV++   ++   +EE+ + E +    +   +   VVEEED  WP DAD GWG   
Sbjct: 101 RERDPEVLEGKGDILKRKEELSDEEGKEGTGEEEGELKAVVEEEDPSWPLDADIGWGIRA 160

Query: 179 GQFFDKITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLI 238
            ++FDK +IKN   D            EI W+ +     +K+I   EWE   F   SPLI
Sbjct: 161 SEYFDKHSIKNVTVDGV----------EIDWEREVEEGWVKEINCLEWESFAFHP-SPLI 220

Query: 239 IFVHNRYKRPKENEKVREELENAIHIIWNC--NLPSPRCVAVDAVVERNLVSALQVSAFP 298
           + V  RY R  +N K  +ELE A  + WN    LP PR V VD  +ER+L  ALQV   P
Sbjct: 221 VLVFERYNRAADNWKFLQELEKAAKVYWNSKDRLP-PRTVKVDMNIERDLAFALQVKECP 280

Query: 299 EIIFTKAGKILYREKGFVSADELSKIMAFFYYGAAKPLCLN 334
           +++F +  KILYREK   +ADEL +++A FYY A +P C+N
Sbjct: 281 QLLFLRGNKILYREKELRTADELVQMIAHFYYNAKRPSCVN 309

BLAST of PI0028074 vs. ExPASy Swiss-Prot
Match: F4JLC1 (Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.7e-29
Identity = 84/241 (34.85%), Postives = 128/241 (53.11%), Query Frame = 0

Query: 99  SSSAKQLDVELMDAEERREWREKIREVIDMNPNV---EEEIDNMERRRKMQKLLADYPLV 158
           S   ++ + + ++   R         + + NP V   +E+ + ++ R   ++   +  +V
Sbjct: 94  SKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKANFIKDRGVDEEEEEEEEMV 153

Query: 159 VEEEDLDWPEDAD-GWGFNLGQFFDKITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPI 218
           VEEED DWP D D GWG    ++FD   IKN   DD          +EI W+ +     +
Sbjct: 154 VEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDD---------GSEIDWEGEIDDSWV 213

Query: 219 KDITISEWEEAVFKDISPLIIFVHNRYKRPKENEKVREELENAIHIIWNC--NLPSPRCV 278
           K+I   EWE   F   SPL++ V  RYKR  +N K  +ELE AI + W+    LP PR V
Sbjct: 214 KEINCLEWESFAFHP-SPLVVLVFERYKRASDNWKTLKELEKAIKVYWDAKDRLP-PRAV 273

Query: 279 AVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSADELSKIMAFFYYGAAKPLCL 334
            +D  +E +L  AL+    P+I+F +  +ILYREK F +ADEL  ++A FYY A +P C+
Sbjct: 274 KIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRTADELVHMIAHFYYKAKRPSCV 323

BLAST of PI0028074 vs. ExPASy TrEMBL
Match: A0A5D3BBT5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1159G00150 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 326/346 (94.22%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQHTLGL SL LPSSNCKFEGGALFVST+S TTNLLSSWTLESSYPSSISFMKFPFRG
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGALFVSTSSHTTNLLSSWTLESSYPSSISFMKFPFRG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
           SCFRSSTLRAYRTAGS++PGDGKKKKGQAGMD DEEDDSSSAKQLD E MDAEER+EWRE
Sbjct: 61  SCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIR+VIDMNPNVEEEIDNMER+RKMQKLLA+YPLVVEEED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           ITIKNKKKD+K+DDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR
Sbjct: 181 ITIKNKKKDNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENEKVR+ELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKAG
Sbjct: 241 YKRPKENEKVRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           KILYREKGFVSADELSKIMAFFYYGAAKP CLNDVGDYQE IPSVS
Sbjct: 301 KILYREKGFVSADELSKIMAFFYYGAAKPPCLNDVGDYQETIPSVS 346

BLAST of PI0028074 vs. ExPASy TrEMBL
Match: A0A1S3B4P3 (uncharacterized protein LOC103485750 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485750 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 326/346 (94.22%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQHTLGL SL LPSSNCKFEGGALFVST+S TTNLLSSWTLESSYPSSISFMKFPFRG
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGALFVSTSSHTTNLLSSWTLESSYPSSISFMKFPFRG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
           SCFRSSTLRAYRTAGS++PGDGKKKKGQAGMD DEEDDSSSAKQLD E MDAEER+EWRE
Sbjct: 61  SCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIR+VIDMNPNVEEEIDNMER+RKMQKLLA+YPLVVEEED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           ITIKNKKKD+K+DDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR
Sbjct: 181 ITIKNKKKDNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENEKVR+ELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKAG
Sbjct: 241 YKRPKENEKVRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           KILYREKGFVSADELSKIMAFFYYGAAKP CLNDVGDYQE IPSVS
Sbjct: 301 KILYREKGFVSADELSKIMAFFYYGAAKPPCLNDVGDYQETIPSVS 346

BLAST of PI0028074 vs. ExPASy TrEMBL
Match: A0A0A0LK68 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G123060 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.9e-180
Identity = 322/347 (92.80%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQH+LGLPSL+LPSSNCKFEGGA FVST+SQ TNL SSWTL+SSYPSSISFM FPFRG
Sbjct: 1   MALQHSLGLPSLLLPSSNCKFEGGASFVSTSSQITNLFSSWTLDSSYPSSISFMNFPFRG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
            CFRSS+LRAYRTAGS+RPGDGKKKK QAGMD DEEDDSSSAK+ DVELMDAEERREWRE
Sbjct: 61  GCFRSSSLRAYRTAGSIRPGDGKKKKEQAGMDSDEEDDSSSAKEPDVELMDAEERREWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIR+VID NPNVEEEIDNMERR KMQKLLADYPLVVEEED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDTNPNVEEEIDNMERRIKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           ITIKNKKKDDKYDDDKDDTDNE+VWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR
Sbjct: 181 ITIKNKKKDDKYDDDKDDTDNEVVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENEKVREELE AIHIIWNCNLPSPRCVAVDAVVE NLV+ALQVSAFPEIIFTKAG
Sbjct: 241 YKRPKENEKVREELEKAIHIIWNCNLPSPRCVAVDAVVECNLVTALQVSAFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVSS 348
           KILYREKGFV+ADELSKIMAFFYYGAAKP CLNDVGDYQEAIPSVSS
Sbjct: 301 KILYREKGFVNADELSKIMAFFYYGAAKPPCLNDVGDYQEAIPSVSS 347

BLAST of PI0028074 vs. ExPASy TrEMBL
Match: A0A6J1DK13 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021817 PE=4 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.2e-159
Identity = 287/346 (82.95%), Postives = 310/346 (89.60%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQH LG P LV+P ++ KF GGALF STNSQT   L +W L+SSYPSSIS +K PF G
Sbjct: 1   MALQHPLGFPFLVMPCAHYKFRGGALFASTNSQTNKFLYTWRLKSSYPSSISLIKPPFLG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
           SCFRSSTLRAY+TAG LR  DGKKKK QA MD DEEDDSSS  + D +LMD++ER EWR+
Sbjct: 61  SCFRSSTLRAYKTAGPLRSDDGKKKKVQADMDSDEEDDSSSEDRQDPDLMDSDERWEWRK 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIREVID NPNVEEEIDNMERRRKMQKLLA+YPLVVEEED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIREVIDRNPNVEEEIDNMERRRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           I+IKNKKKDDKYDDDKDD+DNEIVWQDDNYIRPIKDIT +EWEEAVFKDISPLII VHNR
Sbjct: 181 ISIKNKKKDDKYDDDKDDSDNEIVWQDDNYIRPIKDITTAEWEEAVFKDISPLIILVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENE++REELE AIHIIWNCNLPSPRCVAVDA+VE +LVSALQVS+FPEIIFTKAG
Sbjct: 241 YKRPKENEQIREELEKAIHIIWNCNLPSPRCVAVDAIVEHDLVSALQVSSFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           KIL+REKGFVS DELSKIMAFFYYGAAKP CL D+ DYQEAIPSVS
Sbjct: 301 KILFREKGFVSGDELSKIMAFFYYGAAKPPCLKDIPDYQEAIPSVS 346

BLAST of PI0028074 vs. ExPASy TrEMBL
Match: A0A6J1EMP2 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435768 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.4e-159
Identity = 292/347 (84.15%), Postives = 313/347 (90.20%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGAL-FVSTNSQTTNLLSSWTLESSYPSSISFMKFPFR 60
           MALQHTL LP LVLPSSNC+F+GGA  F +TNSQ ++  +SW L+SSYPSSI+ +K  F 
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSIASVKLSFL 60

Query: 61  GSCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWR 120
           GS FRSS  RAY+TAGSL  GDGKKKKGQA  D DEEDDSSSA+Q D+E MD EERREWR
Sbjct: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120

Query: 121 EKIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFD 180
           EKIREVIDMNP+VEEE+D MERRRKMQKLLADYPLVVEEED +WPEDADGWGFNLGQFFD
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240
           KITIKNKKKDDK DDDKDD D+E+VWQDDNYIRPIKDI  +EWEEAVFKDISPLII VHN
Sbjct: 181 KITIKNKKKDDKDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVHN 240

Query: 241 RYKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKA 300
           RYKRPKENEK+R+ELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKA
Sbjct: 241 RYKRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKA 300

Query: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           GKILYREKGFVSADELSKIMAFFYYGAAKP CL+DV DYQEAIPSVS
Sbjct: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVS 347

BLAST of PI0028074 vs. NCBI nr
Match: XP_008441666.1 (PREDICTED: uncharacterized protein LOC103485750 isoform X1 [Cucumis melo] >KAA0032393.1 uncharacterized protein E6C27_scaffold219G002690 [Cucumis melo var. makuwa] >TYJ96356.1 uncharacterized protein E5676_scaffold1159G00150 [Cucumis melo var. makuwa])

HSP 1 Score: 648.3 bits (1671), Expect = 3.8e-182
Identity = 326/346 (94.22%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQHTLGL SL LPSSNCKFEGGALFVST+S TTNLLSSWTLESSYPSSISFMKFPFRG
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGALFVSTSSHTTNLLSSWTLESSYPSSISFMKFPFRG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
           SCFRSSTLRAYRTAGS++PGDGKKKKGQAGMD DEEDDSSSAKQLD E MDAEER+EWRE
Sbjct: 61  SCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIR+VIDMNPNVEEEIDNMER+RKMQKLLA+YPLVVEEED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           ITIKNKKKD+K+DDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR
Sbjct: 181 ITIKNKKKDNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENEKVR+ELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKAG
Sbjct: 241 YKRPKENEKVRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           KILYREKGFVSADELSKIMAFFYYGAAKP CLNDVGDYQE IPSVS
Sbjct: 301 KILYREKGFVSADELSKIMAFFYYGAAKPPCLNDVGDYQETIPSVS 346

BLAST of PI0028074 vs. NCBI nr
Match: XP_004138945.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucumis sativus] >XP_011649078.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucumis sativus] >KGN61444.1 hypothetical protein Csa_006009 [Cucumis sativus])

HSP 1 Score: 641.0 bits (1652), Expect = 6.1e-180
Identity = 322/347 (92.80%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQH+LGLPSL+LPSSNCKFEGGA FVST+SQ TNL SSWTL+SSYPSSISFM FPFRG
Sbjct: 1   MALQHSLGLPSLLLPSSNCKFEGGASFVSTSSQITNLFSSWTLDSSYPSSISFMNFPFRG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
            CFRSS+LRAYRTAGS+RPGDGKKKK QAGMD DEEDDSSSAK+ DVELMDAEERREWRE
Sbjct: 61  GCFRSSSLRAYRTAGSIRPGDGKKKKEQAGMDSDEEDDSSSAKEPDVELMDAEERREWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIR+VID NPNVEEEIDNMERR KMQKLLADYPLVVEEED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDTNPNVEEEIDNMERRIKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           ITIKNKKKDDKYDDDKDDTDNE+VWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR
Sbjct: 181 ITIKNKKKDDKYDDDKDDTDNEVVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENEKVREELE AIHIIWNCNLPSPRCVAVDAVVE NLV+ALQVSAFPEIIFTKAG
Sbjct: 241 YKRPKENEKVREELEKAIHIIWNCNLPSPRCVAVDAVVECNLVTALQVSAFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVSS 348
           KILYREKGFV+ADELSKIMAFFYYGAAKP CLNDVGDYQEAIPSVSS
Sbjct: 301 KILYREKGFVNADELSKIMAFFYYGAAKPPCLNDVGDYQEAIPSVSS 347

BLAST of PI0028074 vs. NCBI nr
Match: XP_038876051.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida] >XP_038876052.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida] >XP_038876053.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida] >XP_038876054.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida])

HSP 1 Score: 626.3 bits (1614), Expect = 1.6e-175
Identity = 315/346 (91.04%), Postives = 326/346 (94.22%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQHTLGLPSLVLPSSNCKFEGG LFV TNSQT+++LSSW LE SY SSIS MKFP +G
Sbjct: 1   MALQHTLGLPSLVLPSSNCKFEGGTLFVPTNSQTSSVLSSWKLECSYASSISLMKFPSQG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
           SCFRSSTLRAY+TAGSLR GDGKKKKGQ  MD DEEDDSSS KQ D+ELMDAEERREWRE
Sbjct: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQVDMDSDEEDDSSSEKQQDLELMDAEERREWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIREVIDMNPNVEEEIDNME+RR MQKLLADYPLVV+EED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIREVIDMNPNVEEEIDNMEKRRMMQKLLADYPLVVDEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           ITIKNKKKDDK DDDKDDTDNEIVWQDDNYIRPIKDITI+EWEEAVFKDISPLIIFVHNR
Sbjct: 181 ITIKNKKKDDKDDDDKDDTDNEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENEKVREELENA+HIIWNCNLPSPRCVAVDAVVE NLVSALQVS FPEIIFTKAG
Sbjct: 241 YKRPKENEKVREELENAVHIIWNCNLPSPRCVAVDAVVECNLVSALQVSTFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           KILYREKGFVSADELSKIMAFFYYGAAKP CLND+GDYQE IPSVS
Sbjct: 301 KILYREKGFVSADELSKIMAFFYYGAAKPPCLNDIGDYQETIPSVS 346

BLAST of PI0028074 vs. NCBI nr
Match: XP_022154590.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 571.6 bits (1472), Expect = 4.5e-159
Identity = 287/346 (82.95%), Postives = 310/346 (89.60%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGALFVSTNSQTTNLLSSWTLESSYPSSISFMKFPFRG 60
           MALQH LG P LV+P ++ KF GGALF STNSQT   L +W L+SSYPSSIS +K PF G
Sbjct: 1   MALQHPLGFPFLVMPCAHYKFRGGALFASTNSQTNKFLYTWRLKSSYPSSISLIKPPFLG 60

Query: 61  SCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWRE 120
           SCFRSSTLRAY+TAG LR  DGKKKK QA MD DEEDDSSS  + D +LMD++ER EWR+
Sbjct: 61  SCFRSSTLRAYKTAGPLRSDDGKKKKVQADMDSDEEDDSSSEDRQDPDLMDSDERWEWRK 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDK 180
           KIREVID NPNVEEEIDNMERRRKMQKLLA+YPLVVEEED DWPEDADGWGFNLGQFFDK
Sbjct: 121 KIREVIDRNPNVEEEIDNMERRRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNR 240
           I+IKNKKKDDKYDDDKDD+DNEIVWQDDNYIRPIKDIT +EWEEAVFKDISPLII VHNR
Sbjct: 181 ISIKNKKKDDKYDDDKDDSDNEIVWQDDNYIRPIKDITTAEWEEAVFKDISPLIILVHNR 240

Query: 241 YKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAG 300
           YKRPKENE++REELE AIHIIWNCNLPSPRCVAVDA+VE +LVSALQVS+FPEIIFTKAG
Sbjct: 241 YKRPKENEQIREELEKAIHIIWNCNLPSPRCVAVDAIVEHDLVSALQVSSFPEIIFTKAG 300

Query: 301 KILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           KIL+REKGFVS DELSKIMAFFYYGAAKP CL D+ DYQEAIPSVS
Sbjct: 301 KILFREKGFVSGDELSKIMAFFYYGAAKPPCLKDIPDYQEAIPSVS 346

BLAST of PI0028074 vs. NCBI nr
Match: XP_022929067.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata] >XP_022929069.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata] >XP_022929070.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata] >XP_022929071.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita moschata])

HSP 1 Score: 570.1 bits (1468), Expect = 1.3e-158
Identity = 292/347 (84.15%), Postives = 313/347 (90.20%), Query Frame = 0

Query: 1   MALQHTLGLPSLVLPSSNCKFEGGAL-FVSTNSQTTNLLSSWTLESSYPSSISFMKFPFR 60
           MALQHTL LP LVLPSSNC+F+GGA  F +TNSQ ++  +SW L+SSYPSSI+ +K  F 
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSIASVKLSFL 60

Query: 61  GSCFRSSTLRAYRTAGSLRPGDGKKKKGQAGMDLDEEDDSSSAKQLDVELMDAEERREWR 120
           GS FRSS  RAY+TAGSL  GDGKKKKGQA  D DEEDDSSSA+Q D+E MD EERREWR
Sbjct: 61  GSYFRSSPRRAYKTAGSLWSGDGKKKKGQADTDSDEEDDSSSAEQQDLEPMDVEERREWR 120

Query: 121 EKIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFD 180
           EKIREVIDMNP+VEEE+D MERRRKMQKLLADYPLVVEEED +WPEDADGWGFNLGQFFD
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVVEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240
           KITIKNKKKDDK DDDKDD D+E+VWQDDNYIRPIKDI  +EWEEAVFKDISPLII VHN
Sbjct: 181 KITIKNKKKDDKDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVHN 240

Query: 241 RYKRPKENEKVREELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKA 300
           RYKRPKENEK+R+ELENAIHIIWNCNLPSPRCVAVDAVVE NLVSALQVSAFPEIIFTKA
Sbjct: 241 RYKRPKENEKIRKELENAIHIIWNCNLPSPRCVAVDAVVECNLVSALQVSAFPEIIFTKA 300

Query: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSVS 347
           GKILYREKGFVSADELSKIMAFFYYGAAKP CL+DV DYQEAIPSVS
Sbjct: 301 GKILYREKGFVSADELSKIMAFFYYGAAKPPCLSDVRDYQEAIPSVS 347

BLAST of PI0028074 vs. TAIR 10
Match: AT2G31840.1 (Thioredoxin superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 4.6e-101
Identity = 192/274 (70.07%), Postives = 218/274 (79.56%), Query Frame = 0

Query: 82  GKKKKGQAGMDLDEEDDSSSAKQ-----LDVELMDAEERREWREKIREVIDMNPNVE--E 141
           GKK   +   D D ED+    K+      +  L D  ERREWR+ IREVID +P++E  E
Sbjct: 72  GKKVWRKKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIREVIDKHPDIEEDE 131

Query: 142 EIDNMERRRKMQKLLADYPLVVEEEDLDWPEDADGWGFNLGQFFDKITIKNKKK---DDK 201
           EID +E+RRKMQKLLADYPLVV EED +WPEDADGWGF+  QFF+KITIKN+KK   DD 
Sbjct: 132 EIDMVEKRRKMQKLLADYPLVVNEEDPNWPEDADGWGFSFNQFFNKITIKNEKKEEEDDD 191

Query: 202 YDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHNRYKRPKENEKVR 261
            D + DD++ EIVWQDDNYIRPIKD+T +EWEEAVFKDISPL++ VHNRYKRPKENEK R
Sbjct: 192 EDSEGDDSEKEIVWQDDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKRPKENEKFR 251

Query: 262 EELENAIHIIWNCNLPSPRCVAVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVS 321
           EELE AI +IWNC LPSPRCVAVDAVVE +LVSAL+VS FPEIIFTKAGKILYREKG  +
Sbjct: 252 EELEKAIQVIWNCGLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKAGKILYREKGIRT 311

Query: 322 ADELSKIMAFFYYGAAKPLCLNDVGDYQEAIPSV 346
           ADELSKIMAFFYYGAAKP CLN V + QE IP V
Sbjct: 312 ADELSKIMAFFYYGAAKPPCLNGVVNSQEQIPLV 345

BLAST of PI0028074 vs. TAIR 10
Match: AT4G28590.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: Thioredoxin superfamily protein (TAIR:AT2G31840.1); Has 114 Blast hits to 112 proteins in 39 species: Archae - 2; Bacteria - 0; Metazoa - 17; Fungi - 6; Plants - 67; Viruses - 0; Other Eukaryotes - 22 (source: NCBI BLink). )

HSP 1 Score: 131.0 bits (328), Expect = 1.9e-30
Identity = 84/241 (34.85%), Postives = 128/241 (53.11%), Query Frame = 0

Query: 99  SSSAKQLDVELMDAEERREWREKIREVIDMNPNV---EEEIDNMERRRKMQKLLADYPLV 158
           S   ++ + + ++   R         + + NP V   +E+ + ++ R   ++   +  +V
Sbjct: 94  SKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKANFIKDRGVDEEEEEEEEMV 153

Query: 159 VEEEDLDWPEDAD-GWGFNLGQFFDKITIKNKKKDDKYDDDKDDTDNEIVWQDDNYIRPI 218
           VEEED DWP D D GWG    ++FD   IKN   DD          +EI W+ +     +
Sbjct: 154 VEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDD---------GSEIDWEGEIDDSWV 213

Query: 219 KDITISEWEEAVFKDISPLIIFVHNRYKRPKENEKVREELENAIHIIWNC--NLPSPRCV 278
           K+I   EWE   F   SPL++ V  RYKR  +N K  +ELE AI + W+    LP PR V
Sbjct: 214 KEINCLEWESFAFHP-SPLVVLVFERYKRASDNWKTLKELEKAIKVYWDAKDRLP-PRAV 273

Query: 279 AVDAVVERNLVSALQVSAFPEIIFTKAGKILYREKGFVSADELSKIMAFFYYGAAKPLCL 334
            +D  +E +L  AL+    P+I+F +  +ILYREK F +ADEL  ++A FYY A +P C+
Sbjct: 274 KIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRTADELVHMIAHFYYKAKRPSCV 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKB66.5e-10070.07Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidop... [more]
Q0DI482.7e-6952.19Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=... [more]
Q5QLS71.7e-3139.82Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=O... [more]
F4JLC12.7e-2934.85Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidops... [more]
Match NameE-valueIdentityDescription
A0A5D3BBT51.8e-18294.22Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B4P31.8e-18294.22uncharacterized protein LOC103485750 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LK682.9e-18092.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G123060 PE=4 SV=1[more]
A0A6J1DK132.2e-15982.95thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 ... [more]
A0A6J1EMP26.4e-15984.15thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
XP_008441666.13.8e-18294.22PREDICTED: uncharacterized protein LOC103485750 isoform X1 [Cucumis melo] >KAA00... [more]
XP_004138945.16.1e-18092.80thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucumis sa... [more]
XP_038876051.11.6e-17591.04thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa ... [more]
XP_022154590.14.5e-15982.95thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 ... [more]
XP_022929067.11.3e-15884.15thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT2G31840.14.6e-10170.07Thioredoxin superfamily protein [more]
AT4G28590.11.9e-3034.85FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 236..320
e-value: 1.0E-5
score: 27.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..103
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..103
NoneNo IPR availablePANTHERPTHR34669:SF1THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 1..346
IPR044701Thioredoxin-like fold domain-containing protein MRL7/MRL7LPANTHERPTHR34669THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 1..346
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 214..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028074.1PI0028074.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009570 chloroplast stroma