PI0028021 (gene) Melon (PI 482460) v1

Overview
NamePI0028021
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAbhydrolase_3 domain-containing protein
Locationchr05: 22779279 .. 22780330 (+)
RNA-Seq ExpressionPI0028021
SyntenyPI0028021
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTCCCATTGAAGTCGATGAGTGCCGTGGTGTCCTCCGCGTTTACAGCGACGGTTCCATAGTCCGTTCTTCCAATCCAAGCTTCAATGTCCCCGTCCACGACGACGGTTCCGTTCTATGGAAAGACCTTCTCTTTAACCCCATCCACAACCTGCACCTCCGTCTCTACAAACCTGCCGACATTTCTTCTCCCAAGCTTCCCGTCTTTTTCTACATCCACGGCGGCGGCTTCTGCATCGGCTCCCGCACCTGGCCCAATTGCCAAAATTACTGCTTCCGCCTTGCCTCCCAGCTTTCTGTTCTCGTCATCTCCCCTGACTACCGTCTCGCTCCCGAAAATCGCCTTCCGGCTGCCATTGATGACGGTTTCGCAGCTCTTCGGTGGCTCCAAGCCCAAGCTGAATCAGACCACCCTGATCCCTGGCTGGCCAACGTGGCCGATTTCGGCAGAGTTTTCATCAGCGGTGACTCTGCCGGTGGGAACATCGCTCATCATCTTGCGGTGGGACTTGGCCTAGGTTCGCCTGAGTTGGCGCCAGTTCGAGTGAGAGGCTACGTTCTCCTGGGGCCATTCTTCGGCGGAACGGTGAGGACTCAGTCCGAGGCAGAAGGCTCTAAAGAAGCGTTCCTGAATCTGGAACTCATCGACAGGTTGAAATCAGATAATGAAATTTGAATTTGAAGTTTGATTTCGAGTTTTGATTACAGTGAAGTTAGTTTTTGGTTTTGACGATTGAGCAGGTTTTGGAGATTGTCGATCCCGATCGGAAGTAACACTGATCATCCTCTTGTGAATGTGTTCGGTCCGACAAGCCGGAACTTGGTGGCGGTGAAGATGGATCCGATAATGGTGGTGGTGGGCGGCGCCGATTGCTGAAAGATCGAGGGGTGGAGTACGTAGAGGAGCTGAAAAAACAGGGGAAGAAGATAGAATTAGTGGAGTTTGAAGGAAAACAACATGGATTCTTCACCATTGATCCAAATTCTGAAGCTTCTAATCAATTAATGCTTCTCATCAACCATTTTGTAGCTCAACATTCACTCTGA

mRNA sequence

ATGGCCGCTCCCATTGAAGTCGATGAGTGCCGTGGTGTCCTCCGCGTTTACAGCGACGGTTCCATAGTCCGTTCTTCCAATCCAAGCTTCAATGTCCCCGTCCACGACGACGGTTCCGTTCTATGGAAAGACCTTCTCTTTAACCCCATCCACAACCTGCACCTCCGTCTCTACAAACCTGCCGACATTTCTTCTCCCAAGCTTCCCGTCTTTTTCTACATCCACGGCGGCGGCTTCTGCATCGGCTCCCGCACCTGGCCCAATTGCCAAAATTACTGCTTCCGCCTTGCCTCCCAGCTTTCTGTTCTCGTCATCTCCCCTGACTACCGTCTCGCTCCCGAAAATCGCCTTCCGGCTGCCATTGATGACGGTTTCGCAGCTCTTCGGTGGCTCCAAGCCCAAGCTGAATCAGACCACCCTGATCCCTGGCTGGCCAACGTGGCCGATTTCGGCAGAGTTTTCATCAGCGGTGACTCTGCCGGTGGGAACATCGCTCATCATCTTGCGGTGGGACTTGGCCTAGGTTCGCCTGAGTTGGCGCCAGTTCGAGTGAGAGGCTACGTTCTCCTGGGGCCATTCTTCGGCGGAACGGTGAGGACTCAGTCCGAGGCAGAAGGCTCTAAAGAAGCGTTCCTGAATCTGGAACTCATCGACAGGTTTTGGAGATTGTCGATCCCGATCGGAAGTAACACTGATCATCCTCTTGTGAATGTGTTCGGTCCGACAAGCCGGAACTTGGTGGCGGTGAAGATGGATCCGATAATGGTGGTGTACGTAGAGGAGCTGAAAAAACAGGGGAAGAAGATAGAATTAGTGGAGTTTGAAGGAAAACAACATGGATTCTTCACCATTGATCCAAATTCTGAAGCTTCTAATCAATTAATGCTTCTCATCAACCATTTTGTAGCTCAACATTCACTCTGA

Coding sequence (CDS)

ATGGCCGCTCCCATTGAAGTCGATGAGTGCCGTGGTGTCCTCCGCGTTTACAGCGACGGTTCCATAGTCCGTTCTTCCAATCCAAGCTTCAATGTCCCCGTCCACGACGACGGTTCCGTTCTATGGAAAGACCTTCTCTTTAACCCCATCCACAACCTGCACCTCCGTCTCTACAAACCTGCCGACATTTCTTCTCCCAAGCTTCCCGTCTTTTTCTACATCCACGGCGGCGGCTTCTGCATCGGCTCCCGCACCTGGCCCAATTGCCAAAATTACTGCTTCCGCCTTGCCTCCCAGCTTTCTGTTCTCGTCATCTCCCCTGACTACCGTCTCGCTCCCGAAAATCGCCTTCCGGCTGCCATTGATGACGGTTTCGCAGCTCTTCGGTGGCTCCAAGCCCAAGCTGAATCAGACCACCCTGATCCCTGGCTGGCCAACGTGGCCGATTTCGGCAGAGTTTTCATCAGCGGTGACTCTGCCGGTGGGAACATCGCTCATCATCTTGCGGTGGGACTTGGCCTAGGTTCGCCTGAGTTGGCGCCAGTTCGAGTGAGAGGCTACGTTCTCCTGGGGCCATTCTTCGGCGGAACGGTGAGGACTCAGTCCGAGGCAGAAGGCTCTAAAGAAGCGTTCCTGAATCTGGAACTCATCGACAGGTTTTGGAGATTGTCGATCCCGATCGGAAGTAACACTGATCATCCTCTTGTGAATGTGTTCGGTCCGACAAGCCGGAACTTGGTGGCGGTGAAGATGGATCCGATAATGGTGGTGTACGTAGAGGAGCTGAAAAAACAGGGGAAGAAGATAGAATTAGTGGAGTTTGAAGGAAAACAACATGGATTCTTCACCATTGATCCAAATTCTGAAGCTTCTAATCAATTAATGCTTCTCATCAACCATTTTGTAGCTCAACATTCACTCTGA

Protein sequence

MAAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKPADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAIDDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAPVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGPTSRNLVAVKMDPIMVVYVEELKKQGKKIELVEFEGKQHGFFTIDPNSEASNQLMLLINHFVAQHSL
Homology
BLAST of PI0028021 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.8e-84
Identity = 155/319 (48.59%), Postives = 215/319 (67.40%), Query Frame = 0

Query: 4   PIEVDECRGVLRVYSDGSIVRSSNPSF---NVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 63
           P   ++C G+L++ S+G+++RS +       +P  ++ +VL+KD +++  +NLHLRLYKP
Sbjct: 8   PQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP 67

Query: 64  ADISS-PKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPA 123
              S+   LPV  + HGGGFC GSR+WP+  N+C  LAS L+ LV+SPDYRLAPE+RLPA
Sbjct: 68  ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPA 127

Query: 124 AIDDGFAALRWLQAQAESDHPDPWL--ANVADFGRVFISGDSAGGNIAHHLAVGLGLGSP 183
           A +D  A L WL  QA SD  + W       DF RVF+ GDS+GGNIAH LAV  G GS 
Sbjct: 128 AFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSI 187

Query: 184 ELAPVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVN 243
           EL PVRVRGYVL+GPFFGG  RT SE  G  EA L+L+L+D+FWRLS+P G+  DH + N
Sbjct: 188 ELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 247

Query: 244 VFGPTSRNLVAVKMDPIMVV-------------YVEELKKQ-GKKIELVEFEGKQHGFFT 303
            FGPTS  L ++ ++P++V+             Y  +LKK  GK+++ +EFE K+HGF++
Sbjct: 248 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYS 307

BLAST of PI0028021 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.9e-45
Identity = 115/321 (35.83%), Postives = 163/321 (50.78%), Query Frame = 0

Query: 4   PIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP--A 63
           PI ++  R + R     S   S +P+ + P      VL KDL  NP+HN  +RL+ P  A
Sbjct: 23  PIVLNPDRTITRPIQIPSTAASPDPTSSSP------VLTKDLALNPLHNTFVRLFLPRHA 82

Query: 64  DISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAI 123
             +S KLP+  Y HGGGF + S       ++C  +A    V++ S DYRLAPE+RLPAA 
Sbjct: 83  LYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAY 142

Query: 124 DDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAP 183
           DD   AL+W++     D  D WL N ADF   FI G+SAGGNIA+H  +     + EL P
Sbjct: 143 DDAMEALQWIK-----DSRDEWLTNFADFSNCFIMGESAGGNIAYHAGLRAAAVADELLP 202

Query: 184 VRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGP 243
           ++++G VL  P FGG+ RT SE   + ++ L   ++D  W LS+P+G++ DH   N   P
Sbjct: 203 LKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVLDLIWELSLPMGADRDHEYCN---P 262

Query: 244 TSRNLVAVKMDPI-------MVV-------------YVEELKKQGKKIELVEFEGKQHGF 303
           T+ +      D I       MVV               E L+K+G  +      G  H  
Sbjct: 263 TAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKGVDVVAQFDVGGYHAV 322

BLAST of PI0028021 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.0e-44
Identity = 111/288 (38.54%), Postives = 161/288 (55.90%), Query Frame = 0

Query: 7   VDECRGVLRVYSDGSIVRSS-NPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP---AD 66
           VDE  G+++VY DG + RS   P  +  +  +  V   D++ + + N+  RLY P     
Sbjct: 24  VDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTTK 83

Query: 67  ISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAID 126
            S  KLP+  Y HGGGFC+GS +W     +  RL+++   LV+S +YRLAPEN LPAA +
Sbjct: 84  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 143

Query: 127 DGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAPV 186
           DG  A+ WL  +A +D  + W A   DFGR+F++GDSAGGNIA  +A    L SPE   +
Sbjct: 144 DGVNAILWLN-KARND--NLW-AKQCDFGRIFLAGDSAGGNIAQQVAA--RLASPEDLAL 203

Query: 187 RVRGYVLLGPFFGGTVRTQSEAE--GSKEAFLNLELIDRFWRLSIPIGSNTDHP------ 246
           ++ G +L+ PF+ G  RT+SE      K A L L   D +WR+S+P G+N +HP      
Sbjct: 204 KIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPVK 263

Query: 247 LVNVFGPTSRNLVAV-KMDPIMVVYVEELKKQGKKIELVEFEGKQHGF 282
           ++      +R LV V +MD +M   +E        I+ V  +G  H F
Sbjct: 264 MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDVIKRVLHKGVGHAF 305

BLAST of PI0028021 vs. ExPASy Swiss-Prot
Match: R4X244 (Tuliposide A-converting enzyme b1, amyloplastic OS=Tulipa gesneriana OX=13306 GN=TCEA-B1 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.1e-39
Identity = 100/299 (33.44%), Postives = 150/299 (50.17%), Query Frame = 0

Query: 14  LRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP--ADISSPKLPVF 73
           L +Y DG I R    +  +P   +  V  KD++ +P   + +RLY P   D+ S KLPV 
Sbjct: 82  LIIYKDGRIERLKGTTV-IPACPE--VATKDVIIDPATGVSVRLYLPNVVDLPSKKLPVL 141

Query: 74  FYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAIDDGFAALRWL 133
            Y HGGGF I +   PN  NY   LA++  +L++S +YRLAPE+ +PA+ DD  A   W+
Sbjct: 142 VYFHGGGFVIENTGSPNYHNYLTLLAAKSGLLIVSVNYRLAPEHPIPASFDDCMAGFNWV 201

Query: 134 QAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAPVRVRGYVLLG 193
            + +    P+PWLA   D  ++ ISGDSAGG + H++ +    G        + G  L+ 
Sbjct: 202 VSHSAGPAPEPWLARHGDLTQILISGDSAGGTVTHYVLLRADAGV-------IEGAALVH 261

Query: 194 PFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGPTSRNLVAVKM 253
           P+F G+ R +++ E       + E  ++ WRLS P     D PL+N   P + +L  +K 
Sbjct: 262 PYFLGSKRLENQTEE------DFEFHEKLWRLSTPDTEGLDDPLINPLAPGAPSLAGLKC 321

Query: 254 DPIMV-------------VYVEELKKQ--GKKIELVEFEGKQHGFFTIDPNSEASNQLM 296
              +V             +Y + L K   G K ELV  EG  H F   D + + S  +M
Sbjct: 322 KRAVVFVAELDFLVERGRMYYDALVKSGWGGKAELVHQEGVGHVFHLSDYSGDVSVDMM 364

BLAST of PI0028021 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.3e-39
Identity = 108/310 (34.84%), Postives = 159/310 (51.29%), Query Frame = 0

Query: 19  DGSIVRS-SNPSFNVPVHDDGSV---LWKDLLFNPIHNLHLRLYKPA------DISSPKL 78
           DGSI R  SN        D   +   + KDL  N + +  LRLY P+      ++SS KL
Sbjct: 21  DGSITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKL 80

Query: 79  PVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAIDDGFAAL 138
           P+  Y HGGGF + S       ++C  +A  L+ +V+SP YRLAPE+RLPAA DDG  AL
Sbjct: 81  PIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEAL 140

Query: 139 RWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAPVRVRGYV 198
            W++        D W+ + ADF  VF+ G SAGGN+A+++ +       +L+P+++RG +
Sbjct: 141 DWIKTS-----DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLI 200

Query: 199 LLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVN-VFGPTSRNL- 258
           L  PFFGG  R++SE     +      + D  W LS+P+G + DH   N   G  S  L 
Sbjct: 201 LHHPFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLE 260

Query: 259 -----------VAVKMDPIMVVYVEELKKQGKK-IELVEF--EGKQHGFFTIDPNSEASN 303
                      +  + DP++ +  +  K   KK +E+VE    G  HG    DP+   + 
Sbjct: 261 KIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIRDPSKRKT- 320

BLAST of PI0028021 vs. ExPASy TrEMBL
Match: A0A5A7TDB3 (Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00670 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.8e-168
Identity = 292/320 (91.25%), Postives = 302/320 (94.38%), Query Frame = 0

Query: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 60
           MAAP+EVDECRGVLRVYSDGSIVRSSNPSFNVPV+DDGSVLWKDLLF+PI++LHLRLYKP
Sbjct: 1   MAAPVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKP 60

Query: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAA 120
           ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQL  LVISPDYRLAPENRLPAA
Sbjct: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAA 120

Query: 121 IDDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELA 180
           IDDGFAALRWLQAQAESDHPDPWLA+VADF RVFISGDSAGGNIAHHLAVGLGLGSPELA
Sbjct: 121 IDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELA 180

Query: 181 PVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240
           PVRVRGYVLLGPFFGGTVRT+SEAEGSKEAFLNLELIDRFWRLSIPIGS+TDHPLVNVFG
Sbjct: 181 PVRVRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFG 240

Query: 241 PTSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPN 300
           PTSRNL AV+MDPIMVV             Y EELKKQGKKI+LVEFEGKQHGFFTIDPN
Sbjct: 241 PTSRNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPN 300

Query: 301 SEASNQLMLLINHFVAQHSL 308
           SEASNQLMLLINHFVAQHSL
Sbjct: 301 SEASNQLMLLINHFVAQHSL 320

BLAST of PI0028021 vs. ExPASy TrEMBL
Match: A0A1S3BNL4 (probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103491598 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.5e-167
Identity = 291/320 (90.94%), Postives = 301/320 (94.06%), Query Frame = 0

Query: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 60
           MAAP+EVDECRGVLRVYSDGSIVRSSNPSFNVPV+DDGSVLWKDLLF+PI++LHLRLYKP
Sbjct: 1   MAAPVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKP 60

Query: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAA 120
           ADISSPKL VFFYIHGGGFCIGSRTWPNCQNYCFRLASQL  LVISPDYRLAPENRLPAA
Sbjct: 61  ADISSPKLSVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAA 120

Query: 121 IDDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELA 180
           IDDGFAALRWLQAQAESDHPDPWLA+VADF RVFISGDSAGGNIAHHLAVGLGLGSPELA
Sbjct: 121 IDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELA 180

Query: 181 PVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240
           PVRVRGYVLLGPFFGGTVRT+SEAEGSKEAFLNLELIDRFWRLSIPIGS+TDHPLVNVFG
Sbjct: 181 PVRVRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFG 240

Query: 241 PTSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPN 300
           PTSRNL AV+MDPIMVV             Y EELKKQGKKI+LVEFEGKQHGFFTIDPN
Sbjct: 241 PTSRNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPN 300

Query: 301 SEASNQLMLLINHFVAQHSL 308
           SEASNQLMLLINHFVAQHSL
Sbjct: 301 SEASNQLMLLINHFVAQHSL 320

BLAST of PI0028021 vs. ExPASy TrEMBL
Match: A0A0A0KZ31 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G279870 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 6.9e-165
Identity = 288/320 (90.00%), Postives = 297/320 (92.81%), Query Frame = 0

Query: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 60
           MAAPIEVDECRGVLRVYSDGSIVRSSNPSF+VPV DDGSVLWKDLLF+PIHNLHLRLYKP
Sbjct: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFSVPVLDDGSVLWKDLLFDPIHNLHLRLYKP 60

Query: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAA 120
           A ISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLAS+LS LVISPDYRLAPENRLPAA
Sbjct: 61  AHISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASELSALVISPDYRLAPENRLPAA 120

Query: 121 IDDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELA 180
           IDDGFAALRWLQAQAESDHPDPWLA VADF  VFISGDSAGGNIAHHLAVGLG+GSPELA
Sbjct: 121 IDDGFAALRWLQAQAESDHPDPWLAEVADFSTVFISGDSAGGNIAHHLAVGLGVGSPELA 180

Query: 181 PVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240
           PV+VRGYVLLGPFFGGTVRT+SEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG
Sbjct: 181 PVQVRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240

Query: 241 PTSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPN 300
           P S NL AV+MDPI+VV             YVEELKKQGKKI+LVEFE KQHGFFTIDPN
Sbjct: 241 PRSLNLEAVEMDPIVVVVAGADLLKDRAVEYVEELKKQGKKIDLVEFEEKQHGFFTIDPN 300

Query: 301 SEASNQLMLLINHFVAQHSL 308
           SEASNQLMLLINHFVAQHSL
Sbjct: 301 SEASNQLMLLINHFVAQHSL 320

BLAST of PI0028021 vs. ExPASy TrEMBL
Match: A0A6J1JYE3 (probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111490877 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 6.3e-150
Identity = 263/318 (82.70%), Postives = 284/318 (89.31%), Query Frame = 0

Query: 2   AAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKPA 61
           ++P EVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLF+PI++LHLRLYKPA
Sbjct: 4   SSPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVLDDGSVLWKDLLFDPINHLHLRLYKPA 63

Query: 62  DISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAI 121
            +SS  LPVFFYIHGGGFCIGSRTWPNCQNYCFRLA +L  LVISPDYRLAPENRLPAAI
Sbjct: 64  -VSSSALPVFFYIHGGGFCIGSRTWPNCQNYCFRLALELPALVISPDYRLAPENRLPAAI 123

Query: 122 DDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAP 181
           DDGF+A+RWLQAQA+SDHPDPWLA+VADF RVFISGDSAGGNIAHHLAVGLGLGSP  AP
Sbjct: 124 DDGFSAVRWLQAQAQSDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPAFAP 183

Query: 182 VRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGP 241
           VRVRGYVLL PFFGGTVRT+SEAEG K+AFLNLELIDRFWRLSIPIGS+TDHPLVNV G 
Sbjct: 184 VRVRGYVLLAPFFGGTVRTRSEAEGPKDAFLNLELIDRFWRLSIPIGSSTDHPLVNVLGQ 243

Query: 242 TSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPNS 301
           +S++L AV++DPI+VV             Y E LKKQ KKIELVEFEGKQHGFFTIDPNS
Sbjct: 244 SSQDLEAVEVDPILVVVGGADLLKDRAVEYAETLKKQVKKIELVEFEGKQHGFFTIDPNS 303

Query: 302 EASNQLMLLINHFVAQHS 307
           EASNQLMLL+  FVAQHS
Sbjct: 304 EASNQLMLLLKQFVAQHS 320

BLAST of PI0028021 vs. ExPASy TrEMBL
Match: A0A6J1DHZ6 (probable carboxylesterase 15 OS=Momordica charantia OX=3673 GN=LOC111021261 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 253/318 (79.56%), Postives = 278/318 (87.42%), Query Frame = 0

Query: 4   PIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKPADI 63
           P EV+ECRGVLRVYSDGSIVRSSNPSF+VPV DDGSVLWKDLLF+ I+ LH+RLYKPA+ 
Sbjct: 10  PFEVEECRGVLRVYSDGSIVRSSNPSFDVPVLDDGSVLWKDLLFDLINRLHVRLYKPANC 69

Query: 64  SSPK--LPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAI 123
           SS    LP+FFYIHGGGFCIGSRTWPNCQNYCFRLA  LS LVISPDYRLAPENRLPAAI
Sbjct: 70  SSSTSLLPIFFYIHGGGFCIGSRTWPNCQNYCFRLALHLSALVISPDYRLAPENRLPAAI 129

Query: 124 DDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAP 183
            DGFAALRWLQAQA SDHPDPWL++VADF R+FISGDSAGGNIAHHLAVGLG GSPELAP
Sbjct: 130 HDGFAALRWLQAQALSDHPDPWLSHVADFSRIFISGDSAGGNIAHHLAVGLGAGSPELAP 189

Query: 184 VRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGP 243
           +RVRGYVLLGPFFGGTVRT+SEAEG KEAFLNL+LIDRFWRLSIP GS TDHPLVNV GP
Sbjct: 190 LRVRGYVLLGPFFGGTVRTRSEAEGPKEAFLNLDLIDRFWRLSIPPGSTTDHPLVNVLGP 249

Query: 244 TSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPNS 303
            SR+L AV++DP++VV             Y E+LK+QGK IELVEFEGKQHGFFTI PNS
Sbjct: 250 WSRDLEAVELDPMLVVVGGGDLLKDRAVEYAEKLKEQGKMIELVEFEGKQHGFFTIHPNS 309

Query: 304 EASNQLMLLINHFVAQHS 307
           EA+NQLML++N F+ Q+S
Sbjct: 310 EAANQLMLIVNRFITQNS 327

BLAST of PI0028021 vs. NCBI nr
Match: KAA0040076.1 (putative carboxylesterase 15 [Cucumis melo var. makuwa] >TYK21700.1 putative carboxylesterase 15 [Cucumis melo var. makuwa])

HSP 1 Score: 601.7 bits (1550), Expect = 3.6e-168
Identity = 292/320 (91.25%), Postives = 302/320 (94.38%), Query Frame = 0

Query: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 60
           MAAP+EVDECRGVLRVYSDGSIVRSSNPSFNVPV+DDGSVLWKDLLF+PI++LHLRLYKP
Sbjct: 1   MAAPVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKP 60

Query: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAA 120
           ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQL  LVISPDYRLAPENRLPAA
Sbjct: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAA 120

Query: 121 IDDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELA 180
           IDDGFAALRWLQAQAESDHPDPWLA+VADF RVFISGDSAGGNIAHHLAVGLGLGSPELA
Sbjct: 121 IDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELA 180

Query: 181 PVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240
           PVRVRGYVLLGPFFGGTVRT+SEAEGSKEAFLNLELIDRFWRLSIPIGS+TDHPLVNVFG
Sbjct: 181 PVRVRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFG 240

Query: 241 PTSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPN 300
           PTSRNL AV+MDPIMVV             Y EELKKQGKKI+LVEFEGKQHGFFTIDPN
Sbjct: 241 PTSRNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPN 300

Query: 301 SEASNQLMLLINHFVAQHSL 308
           SEASNQLMLLINHFVAQHSL
Sbjct: 301 SEASNQLMLLINHFVAQHSL 320

BLAST of PI0028021 vs. NCBI nr
Match: XP_008449839.1 (PREDICTED: probable carboxylesterase 15 [Cucumis melo])

HSP 1 Score: 598.6 bits (1542), Expect = 3.1e-167
Identity = 291/320 (90.94%), Postives = 301/320 (94.06%), Query Frame = 0

Query: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 60
           MAAP+EVDECRGVLRVYSDGSIVRSSNPSFNVPV+DDGSVLWKDLLF+PI++LHLRLYKP
Sbjct: 1   MAAPVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKP 60

Query: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAA 120
           ADISSPKL VFFYIHGGGFCIGSRTWPNCQNYCFRLASQL  LVISPDYRLAPENRLPAA
Sbjct: 61  ADISSPKLSVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAA 120

Query: 121 IDDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELA 180
           IDDGFAALRWLQAQAESDHPDPWLA+VADF RVFISGDSAGGNIAHHLAVGLGLGSPELA
Sbjct: 121 IDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELA 180

Query: 181 PVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240
           PVRVRGYVLLGPFFGGTVRT+SEAEGSKEAFLNLELIDRFWRLSIPIGS+TDHPLVNVFG
Sbjct: 181 PVRVRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFG 240

Query: 241 PTSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPN 300
           PTSRNL AV+MDPIMVV             Y EELKKQGKKI+LVEFEGKQHGFFTIDPN
Sbjct: 241 PTSRNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPN 300

Query: 301 SEASNQLMLLINHFVAQHSL 308
           SEASNQLMLLINHFVAQHSL
Sbjct: 301 SEASNQLMLLINHFVAQHSL 320

BLAST of PI0028021 vs. NCBI nr
Match: XP_004142252.1 (probable carboxylesterase 15 [Cucumis sativus] >KGN54059.1 hypothetical protein Csa_018062 [Cucumis sativus])

HSP 1 Score: 589.7 bits (1519), Expect = 1.4e-164
Identity = 288/320 (90.00%), Postives = 297/320 (92.81%), Query Frame = 0

Query: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 60
           MAAPIEVDECRGVLRVYSDGSIVRSSNPSF+VPV DDGSVLWKDLLF+PIHNLHLRLYKP
Sbjct: 1   MAAPIEVDECRGVLRVYSDGSIVRSSNPSFSVPVLDDGSVLWKDLLFDPIHNLHLRLYKP 60

Query: 61  ADISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAA 120
           A ISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLAS+LS LVISPDYRLAPENRLPAA
Sbjct: 61  AHISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASELSALVISPDYRLAPENRLPAA 120

Query: 121 IDDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELA 180
           IDDGFAALRWLQAQAESDHPDPWLA VADF  VFISGDSAGGNIAHHLAVGLG+GSPELA
Sbjct: 121 IDDGFAALRWLQAQAESDHPDPWLAEVADFSTVFISGDSAGGNIAHHLAVGLGVGSPELA 180

Query: 181 PVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240
           PV+VRGYVLLGPFFGGTVRT+SEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG
Sbjct: 181 PVQVRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFG 240

Query: 241 PTSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPN 300
           P S NL AV+MDPI+VV             YVEELKKQGKKI+LVEFE KQHGFFTIDPN
Sbjct: 241 PRSLNLEAVEMDPIVVVVAGADLLKDRAVEYVEELKKQGKKIDLVEFEEKQHGFFTIDPN 300

Query: 301 SEASNQLMLLINHFVAQHSL 308
           SEASNQLMLLINHFVAQHSL
Sbjct: 301 SEASNQLMLLINHFVAQHSL 320

BLAST of PI0028021 vs. NCBI nr
Match: XP_038900705.1 (probable carboxylesterase 15 [Benincasa hispida])

HSP 1 Score: 577.4 bits (1487), Expect = 7.3e-161
Identity = 279/317 (88.01%), Postives = 291/317 (91.80%), Query Frame = 0

Query: 4   PIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKPADI 63
           P EVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLF+PIH+LHLRLYKPA I
Sbjct: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASI 64

Query: 64  SSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAIDD 123
           SS KLPV FYIHGGGFCIGSRTWPNCQNYCFRLA++L  LVISPDYRLAPENRLPAAIDD
Sbjct: 65  SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDD 124

Query: 124 GFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAPVR 183
           GFAALRWLQAQAESDHPDPWLA+VADF RVFISGDSAGGNIAHHLA GLGLGSPELAPVR
Sbjct: 125 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVR 184

Query: 184 VRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGPTS 243
           VRGYVLL PFFGGTVRT+SEAEGSKEAFLNLELIDRFWRLSIPIGS+ DHPLV+VFGP+S
Sbjct: 185 VRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSS 244

Query: 244 RNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPNSEA 303
           RNL AV+MDPIMVV             Y E+LKKQGKKIELVEFEGKQHGFFTIDPNSEA
Sbjct: 245 RNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEA 304

Query: 304 SNQLMLLINHFVAQHSL 308
           SNQLMLLINHF+AQHSL
Sbjct: 305 SNQLMLLINHFIAQHSL 321

BLAST of PI0028021 vs. NCBI nr
Match: XP_023534075.1 (probable carboxylesterase 15 [Cucurbita pepo subsp. pepo] >KAG6605861.1 putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035811.1 putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542.0 bits (1395), Expect = 3.4e-150
Identity = 264/318 (83.02%), Postives = 285/318 (89.62%), Query Frame = 0

Query: 2   AAPIEVDECRGVLRVYSDGSIVRSSNPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKPA 61
           ++P EVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLF+PI++LHLRLYKPA
Sbjct: 4   SSPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVLDDGSVLWKDLLFDPINHLHLRLYKPA 63

Query: 62  DISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAI 121
            +SS  LPVFFYIHGGGFCIGSRTWPNCQNYCFRLA +L  LVISPDYRLAPENRLPAAI
Sbjct: 64  -VSSSTLPVFFYIHGGGFCIGSRTWPNCQNYCFRLALELPALVISPDYRLAPENRLPAAI 123

Query: 122 DDGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAP 181
           D GFAA+RWLQAQA+SDHPDPWLA+VADF RVFISGDSAGGNIAHHLAVGLGLGSP LAP
Sbjct: 124 DGGFAAVRWLQAQAQSDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPALAP 183

Query: 182 VRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGP 241
           VRVRGYVLL PFFGGTVRT+SEAEG K+AFLNLELIDRFWRLSIPIGS+TDHPLVNV G 
Sbjct: 184 VRVRGYVLLAPFFGGTVRTRSEAEGPKDAFLNLELIDRFWRLSIPIGSSTDHPLVNVLGQ 243

Query: 242 TSRNLVAVKMDPIMVV-------------YVEELKKQGKKIELVEFEGKQHGFFTIDPNS 301
           +S++L AV++DPI+VV             Y E LKKQGKKIELVEFEGKQHGFFTIDPNS
Sbjct: 244 SSQDLEAVEVDPILVVVGGADLLKDRAVEYAETLKKQGKKIELVEFEGKQHGFFTIDPNS 303

Query: 302 EASNQLMLLINHFVAQHS 307
           EASNQLMLL+  FVA+HS
Sbjct: 304 EASNQLMLLLKQFVARHS 320

BLAST of PI0028021 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 4.1e-85
Identity = 155/319 (48.59%), Postives = 215/319 (67.40%), Query Frame = 0

Query: 4   PIEVDECRGVLRVYSDGSIVRSSNPSF---NVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 63
           P   ++C G+L++ S+G+++RS +       +P  ++ +VL+KD +++  +NLHLRLYKP
Sbjct: 8   PQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP 67

Query: 64  ADISS-PKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPA 123
              S+   LPV  + HGGGFC GSR+WP+  N+C  LAS L+ LV+SPDYRLAPE+RLPA
Sbjct: 68  ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPA 127

Query: 124 AIDDGFAALRWLQAQAESDHPDPWL--ANVADFGRVFISGDSAGGNIAHHLAVGLGLGSP 183
           A +D  A L WL  QA SD  + W       DF RVF+ GDS+GGNIAH LAV  G GS 
Sbjct: 128 AFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSI 187

Query: 184 ELAPVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVN 243
           EL PVRVRGYVL+GPFFGG  RT SE  G  EA L+L+L+D+FWRLS+P G+  DH + N
Sbjct: 188 ELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 247

Query: 244 VFGPTSRNLVAVKMDPIMVV-------------YVEELKKQ-GKKIELVEFEGKQHGFFT 303
            FGPTS  L ++ ++P++V+             Y  +LKK  GK+++ +EFE K+HGF++
Sbjct: 248 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYS 307

BLAST of PI0028021 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 4.1e-85
Identity = 155/319 (48.59%), Postives = 215/319 (67.40%), Query Frame = 0

Query: 4   PIEVDECRGVLRVYSDGSIVRSSNPSF---NVPVHDDGSVLWKDLLFNPIHNLHLRLYKP 63
           P   ++C G+L++ S+G+++RS +       +P  ++ +VL+KD +++  +NLHLRLYKP
Sbjct: 8   PQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP 67

Query: 64  ADISS-PKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPA 123
              S+   LPV  + HGGGFC GSR+WP+  N+C  LAS L+ LV+SPDYRLAPE+RLPA
Sbjct: 68  ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPA 127

Query: 124 AIDDGFAALRWLQAQAESDHPDPWL--ANVADFGRVFISGDSAGGNIAHHLAVGLGLGSP 183
           A +D  A L WL  QA SD  + W       DF RVF+ GDS+GGNIAH LAV  G GS 
Sbjct: 128 AFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSI 187

Query: 184 ELAPVRVRGYVLLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVN 243
           EL PVRVRGYVL+GPFFGG  RT SE  G  EA L+L+L+D+FWRLS+P G+  DH + N
Sbjct: 188 ELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 247

Query: 244 VFGPTSRNLVAVKMDPIMVV-------------YVEELKKQ-GKKIELVEFEGKQHGFFT 303
            FGPTS  L ++ ++P++V+             Y  +LKK  GK+++ +EFE K+HGF++
Sbjct: 248 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYS 307

BLAST of PI0028021 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-45
Identity = 111/288 (38.54%), Postives = 161/288 (55.90%), Query Frame = 0

Query: 7   VDECRGVLRVYSDGSIVRSS-NPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP---AD 66
           VDE  G+++VY DG + RS   P  +  +  +  V   D++ + + N+  RLY P     
Sbjct: 24  VDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTTK 83

Query: 67  ISSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAID 126
            S  KLP+  Y HGGGFC+GS +W     +  RL+++   LV+S +YRLAPEN LPAA +
Sbjct: 84  SSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYE 143

Query: 127 DGFAALRWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAPV 186
           DG  A+ WL  +A +D  + W A   DFGR+F++GDSAGGNIA  +A    L SPE   +
Sbjct: 144 DGVNAILWLN-KARND--NLW-AKQCDFGRIFLAGDSAGGNIAQQVAA--RLASPEDLAL 203

Query: 187 RVRGYVLLGPFFGGTVRTQSEAE--GSKEAFLNLELIDRFWRLSIPIGSNTDHP------ 246
           ++ G +L+ PF+ G  RT+SE      K A L L   D +WR+S+P G+N +HP      
Sbjct: 204 KIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPVK 263

Query: 247 LVNVFGPTSRNLVAV-KMDPIMVVYVEELKKQGKKIELVEFEGKQHGF 282
           ++      +R LV V +MD +M   +E        I+ V  +G  H F
Sbjct: 264 MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDVIKRVLHKGVGHAF 305

BLAST of PI0028021 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 163.7 bits (413), Expect = 2.3e-40
Identity = 108/310 (34.84%), Postives = 159/310 (51.29%), Query Frame = 0

Query: 19  DGSIVRS-SNPSFNVPVHDDGSV---LWKDLLFNPIHNLHLRLYKPA------DISSPKL 78
           DGSI R  SN        D   +   + KDL  N + +  LRLY P+      ++SS KL
Sbjct: 21  DGSITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKL 80

Query: 79  PVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAIDDGFAAL 138
           P+  Y HGGGF + S       ++C  +A  L+ +V+SP YRLAPE+RLPAA DDG  AL
Sbjct: 81  PIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEAL 140

Query: 139 RWLQAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPELAPVRVRGYV 198
            W++        D W+ + ADF  VF+ G SAGGN+A+++ +       +L+P+++RG +
Sbjct: 141 DWIKTS-----DDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLI 200

Query: 199 LLGPFFGGTVRTQSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVN-VFGPTSRNL- 258
           L  PFFGG  R++SE     +      + D  W LS+P+G + DH   N   G  S  L 
Sbjct: 201 LHHPFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLE 260

Query: 259 -----------VAVKMDPIMVVYVEELKKQGKK-IELVEF--EGKQHGFFTIDPNSEASN 303
                      +  + DP++ +  +  K   KK +E+VE    G  HG    DP+   + 
Sbjct: 261 KIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEIRDPSKRKT- 320

BLAST of PI0028021 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 163.3 bits (412), Expect = 3.1e-40
Identity = 101/321 (31.46%), Postives = 164/321 (51.09%), Query Frame = 0

Query: 7   VDECRGVLRVYSDGSIVRSS-NPSFNVPVHDDGSVLWKDLLFNPIHNLHLRLYKP-ADIS 66
           V+E  G+++V++DG + R    P  +  +H        D+  +  ++   R+Y P A  +
Sbjct: 29  VEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLS--NDTWTRVYIPDAAAA 88

Query: 67  SPK--LPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLSVLVISPDYRLAPENRLPAAID 126
           SP   LP+  Y HGGGFC+GS  W    ++   LA +   +++S +YRLAPE+RLPAA D
Sbjct: 89  SPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYD 148

Query: 127 DGFAALRWL---QAQAESDHPDPWLANVADFGRVFISGDSAGGNIAHHLAVGLGLGSPEL 186
           DG   + WL   Q      +P  WL+   +   VF++GDSAG NIA+ +AV +       
Sbjct: 149 DGVNVVSWLVKQQISTGGGYPS-WLSK-CNLSNVFLAGDSAGANIAYQVAVRIMASGKYA 208

Query: 187 APVRVRGYVLLGPFFGGTVRTQSEAE--GSKEAFLNLELIDRFWRLSIPIGSNTDHPLVN 246
             + ++G +L+ PFFGG  RT SE +   +K + L L   D +WRL++P G++ DHP  N
Sbjct: 209 NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWCN 268

Query: 247 VFGPTSRNLVAVKMDPIMVVYVE-------------ELKKQGKKIELVEFEGKQHGFFTI 303
                  +    K+   MV   E              ++  GK++E +   G  H F  +
Sbjct: 269 PL----MSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHIL 328

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FG135.8e-8448.59Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV76.9e-4535.83Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q9SX252.0e-4438.54Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
R4X2441.1e-3933.44Tuliposide A-converting enzyme b1, amyloplastic OS=Tulipa gesneriana OX=13306 GN... [more]
Q9LVB83.3e-3934.84Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TDB31.8e-16891.25Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BNL41.5e-16790.94probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103491598 PE=4 SV=1[more]
A0A0A0KZ316.9e-16590.00Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G2798... [more]
A0A6J1JYE36.3e-15082.70probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111490877 PE=4 SV... [more]
A0A6J1DHZ61.0e-14479.56probable carboxylesterase 15 OS=Momordica charantia OX=3673 GN=LOC111021261 PE=4... [more]
Match NameE-valueIdentityDescription
KAA0040076.13.6e-16891.25putative carboxylesterase 15 [Cucumis melo var. makuwa] >TYK21700.1 putative car... [more]
XP_008449839.13.1e-16790.94PREDICTED: probable carboxylesterase 15 [Cucumis melo][more]
XP_004142252.11.4e-16490.00probable carboxylesterase 15 [Cucumis sativus] >KGN54059.1 hypothetical protein ... [more]
XP_038900705.17.3e-16188.01probable carboxylesterase 15 [Benincasa hispida][more]
XP_023534075.13.4e-15083.02probable carboxylesterase 15 [Cucurbita pepo subsp. pepo] >KAG6605861.1 putative... [more]
Match NameE-valueIdentityDescription
AT5G06570.14.1e-8548.59alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.24.1e-8548.59alpha/beta-Hydrolases superfamily protein [more]
AT1G68620.11.4e-4538.54alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.12.3e-4034.84carboxyesterase 20 [more]
AT5G16080.13.1e-4031.46carboxyesterase 17 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 13..306
e-value: 1.2E-73
score: 250.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 5..305
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 72..282
e-value: 2.6E-47
score: 161.4
NoneNo IPR availablePANTHERPTHR23024:SF416CARBOXYLESTERASE 15-RELATEDcoord: 7..302
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 7..302
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 153..165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028021.1PI0028021.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity