PI0027942 (gene) Melon (PI 482460) v1

BLAST of PI0027942 vs. ExPASy TrEMBL
Match: A0A5A7UDN3 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008660 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 1.1e-106
Identity = 232/307 (75.57%), Postives = 252/307 (82.08%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           MTVTEREE+ NSSN  IVEIKKEPVEF+  Q+EQ+ IDALLN  LK   F D QLQ++ E
Sbjct: 1   MTVTEREEEGNSSNTNIVEIKKEPVEFD--QEEQDGIDALLNLALKDGCFSDDQLQIDNE 60

Query: 61  ----EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDFIN 120
               EESERSTKR RTAESDEA+S +IKNT NL ANQ ELGMDPLE++EEIVLKY DFIN
Sbjct: 61  EEDTEESERSTKRTRTAESDEAESLAIKNTGNLEANQPELGMDPLEVMEEIVLKYHDFIN 120

Query: 121 EIYQMLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWRDILDRGNLLVETLNRSLKTVAIE 180
           EIYQML        KD QKD+Q DEHKKVKIQWQKWR ILDRGNLLVE LNRSLKTV IE
Sbjct: 121 EIYQML--------KDDQKDDQKDEHKKVKIQWQKWRGILDRGNLLVEVLNRSLKTVEIE 180

Query: 181 MEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDEVLTMCLDE 240
           MEWMKSI+ LHREYSIR+THVPDLLALLRDINERIE SP+FKAVSDM K DEVL MCLDE
Sbjct: 181 MEWMKSIEGLHREYSIRRTHVPDLLALLRDINERIELSPHFKAVSDMTKRDEVLPMCLDE 240

Query: 241 LETSKEELAEMVEVIQELKELDLEDDEAVEDGEAVGEEEEIGVEEFEKFNLELDEILKEM 300
           LETSKEEL EMVEVIQELK LDL+ DEAVED    GE EEIG++E EK N E DE+LKE+
Sbjct: 241 LETSKEELTEMVEVIQELKVLDLDKDEAVED----GEREEIGIDEIEKVNAEFDEVLKEI 293

Query: 301 GIEVDEE 304
           GIE+D E
Sbjct: 301 GIELDME 293

BLAST of PI0027942 vs. ExPASy TrEMBL
Match: A0A0A0KKJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G521060 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 2.7e-100
Identity = 221/292 (75.68%), Postives = 238/292 (81.51%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFE-HQQQEQEDIDALLNALLKSAYFYDHQLQLEI 60
           MTVTEREE+ NSSN  IVEIK EPVEF   QQ++QE+IDAL + LLK AYFYD QLQ+EI
Sbjct: 1   MTVTEREEEGNSSNTYIVEIKNEPVEFVLLQQEKQEEIDALSSVLLKGAYFYDDQLQIEI 60

Query: 61  E-----EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDF 120
           E     EESERSTKRIRTAESDE +SSSIKNTA+L  NQ ELG+DPLE++EEIVLKY DF
Sbjct: 61  EKEEDAEESERSTKRIRTAESDEVESSSIKNTASLEENQPELGIDPLEVLEEIVLKYHDF 120

Query: 121 INEIYQMLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWRDILDRGNLLVETLNRSLKTVA 180
           INEIYQML DEQ                KKVKIQWQKWR ILD+GNL+VE L RSLKTV 
Sbjct: 121 INEIYQMLKDEQ----------------KKVKIQWQKWRYILDKGNLIVEDLTRSLKTVE 180

Query: 181 IEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDEVLTMCL 240
           IEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFK VSDMKK DEVL MCL
Sbjct: 181 IEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKVVSDMKKRDEVLKMCL 240

Query: 241 DELETSKEELAEMVEVIQELKELDLEDDEAVEDGEAVGEEEEIGVEEFEKFN 287
           DELETS+EELAEM EVIQELKELDL+DDEAVED    GEEEEI V+  +K N
Sbjct: 241 DELETSQEELAEMFEVIQELKELDLQDDEAVED----GEEEEIKVDALQKIN 272

BLAST of PI0027942 vs. ExPASy TrEMBL
Match: A0A0A0KIE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G521050 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 3.8e-91
Identity = 238/457 (52.08%), Postives = 257/457 (56.24%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           MT TEREE+ NSS   IVEIK E VEFE  + EQEDI+ LLN   K AYFYD Q Q+EIE
Sbjct: 1   MTATEREEEGNSSTTNIVEIKNELVEFE-PEPEQEDINGLLNLASKDAYFYDGQPQIEIE 60

Query: 61  -----EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDFI 120
                EESERSTKRIRTAE DE +SSSIKN +NL  NQ ELG+DPLE++EEI+LKY D I
Sbjct: 61  KEEDAEESERSTKRIRTAEPDEEESSSIKNASNLDVNQLELGIDPLEVMEEIILKYHDLI 120

Query: 121 NEIYQMLTDEQKDKQKDKQK---------------------------------------- 180
           NEIYQML DEQKD+QKDKQK                                        
Sbjct: 121 NEIYQMLKDEQKDEQKDKQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 DEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQK 240

Query: 241 ------------------------------------------------DEQTDEHKKVKI 300
                                                           DEQTDEHKKVKI
Sbjct: 241 DEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQKDEQEDEQEDEQTDEHKKVKI 300

Query: 301 QWQKWRDILDRGNLLVETLNRSLKTVAIEMEWMKSIDELHREYSIRKTHVPDLLALLRDI 303
           QWQKWRDILD+GNL+VE LN SLKTV IEMEWMKS+DELHREYSIRKTHVPDLLALLRDI
Sbjct: 301 QWQKWRDILDKGNLMVEDLNGSLKTVEIEMEWMKSVDELHREYSIRKTHVPDLLALLRDI 360

BLAST of PI0027942 vs. ExPASy TrEMBL
Match: A0A0A0KE23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G184200 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.8e-24
Identity = 95/260 (36.54%), Postives = 144/260 (55.38%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           MTV +REEK N S+             E  ++E++ I    N     +   +   +L I 
Sbjct: 1   MTVRKREEKDNHSSNN-----------EESKKEEDHI----NLQTAQSNSQNQNGKLPIN 60

Query: 61  EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDFINEIYQ 120
           EESERSTKR R  ES+ A S+S   T +       LG DPL +  +I+ KY  FI+ +YQ
Sbjct: 61  EESERSTKRSRITESNVAGSTSTNTTIS-------LGQDPLLVAMQILWKYYKFIDYLYQ 120

Query: 121 MLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWRDILDRGNLLVETLNRSLKTVAIEMEWM 180
           +L ++              +E + +K+QWQKW +IL  G  LV  L + L+T+ +EME +
Sbjct: 121 ILKND--------------EEKQDLKVQWQKWIEILVGGRRLVMGLYQDLRTLILEMESV 180

Query: 181 KSIDELH-REYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDEVLTMCLDELET 240
           K  + L  +E+ ++KT +  +L L R IN RI SS N + VSD+K   +V+ MCL ELE 
Sbjct: 181 KHPEGLEKKEHELKKTQILSILNLFRHINSRIASSSNLRLVSDIKNRGKVMAMCLRELER 224

Query: 241 SKEELAEMVEVIQELKELDL 260
           SK+EL +++ +++ L+E  L
Sbjct: 241 SKQELIQLIGLMKVLREKGL 224

BLAST of PI0027942 vs. ExPASy TrEMBL
Match: A0A5D3CBX0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold615G00350 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 9.0e-24
Identity = 97/278 (34.89%), Postives = 154/278 (55.40%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           M+ TE E+  NSSN  + +IKKE +    Q+QEQ+     +N  + +    +    +   
Sbjct: 1   MSDTENEDSGNSSNTSMADIKKENI----QEQEQD-----INLDICTLNCEEEIDIISTS 60

Query: 61  EESERSTKRIRTAESDEAQSS------SIKNTANLGANQSELGMDPLELIEEIVLKYQDF 120
           +E ERS KR R   S+EA+SS      S  N  +  +  +E+G DPL + EE++LKY +F
Sbjct: 61  DELERSPKRPRITGSNEAESSKKNEQESEYNPDSDTSTFNEIGQDPLLVTEEVLLKYYEF 120

Query: 121 INEIYQMLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWR-DILDRGNLLVETLNRSLKTV 180
           I+ +YQ+L               + DE K      Q W  +I+DR + LV TL R L+ V
Sbjct: 121 IDYVYQIL---------------KNDEEK------QDWSFEIIDRVDTLVVTLGRRLRVV 180

Query: 181 AIEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDEVLTMC 240
           A++M  ++++DEL   Y  +KTH+P +L+ +R +N  I SS +F+ VSD+K   +VL+ C
Sbjct: 181 ALDMAGLEAVDELQNIYEFKKTHIPVILSAIRQVNVEIASSSSFRLVSDIKNRGKVLSNC 240

Query: 241 LDELETSKEELAEMVEVIQELK----ELDLEDDEAVED 268
           L E E  ++EL++ ++ +Q +K    E+ LE     ED
Sbjct: 241 LGEFERWRQELSQRIDAMQPMKRRAMEIALETTSEEED 248

BLAST of PI0027942 vs. NCBI nr
Match: KAA0052577.1 (hypothetical protein E6C27_scaffold120G001820 [Cucumis melo var. makuwa] >TYK13248.1 hypothetical protein E5676_scaffold255G008660 [Cucumis melo var. makuwa])

HSP 1 Score: 396.4 bits (1017), Expect = 2.3e-106
Identity = 232/307 (75.57%), Postives = 252/307 (82.08%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           MTVTEREE+ NSSN  IVEIKKEPVEF+  Q+EQ+ IDALLN  LK   F D QLQ++ E
Sbjct: 1   MTVTEREEEGNSSNTNIVEIKKEPVEFD--QEEQDGIDALLNLALKDGCFSDDQLQIDNE 60

Query: 61  ----EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDFIN 120
               EESERSTKR RTAESDEA+S +IKNT NL ANQ ELGMDPLE++EEIVLKY DFIN
Sbjct: 61  EEDTEESERSTKRTRTAESDEAESLAIKNTGNLEANQPELGMDPLEVMEEIVLKYHDFIN 120

Query: 121 EIYQMLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWRDILDRGNLLVETLNRSLKTVAIE 180
           EIYQML        KD QKD+Q DEHKKVKIQWQKWR ILDRGNLLVE LNRSLKTV IE
Sbjct: 121 EIYQML--------KDDQKDDQKDEHKKVKIQWQKWRGILDRGNLLVEVLNRSLKTVEIE 180

Query: 181 MEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDEVLTMCLDE 240
           MEWMKSI+ LHREYSIR+THVPDLLALLRDINERIE SP+FKAVSDM K DEVL MCLDE
Sbjct: 181 MEWMKSIEGLHREYSIRRTHVPDLLALLRDINERIELSPHFKAVSDMTKRDEVLPMCLDE 240

Query: 241 LETSKEELAEMVEVIQELKELDLEDDEAVEDGEAVGEEEEIGVEEFEKFNLELDEILKEM 300
           LETSKEEL EMVEVIQELK LDL+ DEAVED    GE EEIG++E EK N E DE+LKE+
Sbjct: 241 LETSKEELTEMVEVIQELKVLDLDKDEAVED----GEREEIGIDEIEKVNAEFDEVLKEI 293

Query: 301 GIEVDEE 304
           GIE+D E
Sbjct: 301 GIELDME 293

BLAST of PI0027942 vs. NCBI nr
Match: XP_011658523.2 (rho GTPase-activating protein gacV [Cucumis sativus] >KAE8647736.1 hypothetical protein Csa_004393 [Cucumis sativus])

HSP 1 Score: 385.2 bits (988), Expect = 5.3e-103
Identity = 237/345 (68.70%), Postives = 256/345 (74.20%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           MT TEREE+ NSS   IVEIK E VEFE  + EQEDI+ LLN   K AYFYD Q Q+EIE
Sbjct: 1   MTATEREEEGNSSTTNIVEIKNELVEFE-PEPEQEDINGLLNLASKDAYFYDGQPQIEIE 60

Query: 61  -----EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDFI 120
                EESERSTKRIRTAE DE +SSSIKN +NL  NQ ELG+DPLE++EEI+LKY D I
Sbjct: 61  KEEDAEESERSTKRIRTAEPDEEESSSIKNASNLDVNQLELGIDPLEVMEEIILKYHDLI 120

Query: 121 NEIYQMLTDEQKDKQKDKQK------------------------------------DEQT 180
           NEIYQML DEQKD+QKDKQK                                    DEQT
Sbjct: 121 NEIYQMLKDEQKDEQKDKQKDEQKDEQKDEQKDEQKDEQQDEQKDEQKDEQEDEQEDEQT 180

Query: 181 DEHKKVKIQWQKWRDILDRGNLLVETLNRSLKTVAIEMEWMKSIDELHREYSIRKTHVPD 240
           DEHKKVKIQWQKWRDILD+GNL+VE LN SLKTV IEMEWMKS+DELHREYSIRKTHVPD
Sbjct: 181 DEHKKVKIQWQKWRDILDKGNLMVEDLNGSLKTVEIEMEWMKSVDELHREYSIRKTHVPD 240

Query: 241 LLALLRDINERIESSPNFKAVSDMKKGDEVLTMCLDELETSKEELAEMVEVIQELKELDL 300
           LLALLRDINERIESSPNFK VSDMKK D VL MCLDELETS+EELAEMVEVIQELKEL L
Sbjct: 241 LLALLRDINERIESSPNFKVVSDMKKRDGVLKMCLDELETSQEELAEMVEVIQELKELHL 300

Query: 301 EDDEAVEDGEAVGEE--EEIGVEEFEKFNLELDEILKEMGIEVDE 303
           +DD   ED EAV     EEIGVEE EKFN E DE+LKEM  E+D+
Sbjct: 301 QDD---EDDEAVAGRILEEIGVEEVEKFNAEFDEVLKEMD-ELDD 340

BLAST of PI0027942 vs. NCBI nr
Match: KGN49359.1 (hypothetical protein Csa_002997 [Cucumis sativus])

HSP 1 Score: 375.2 bits (962), Expect = 5.5e-100
Identity = 221/292 (75.68%), Postives = 238/292 (81.51%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFE-HQQQEQEDIDALLNALLKSAYFYDHQLQLEI 60
           MTVTEREE+ NSSN  IVEIK EPVEF   QQ++QE+IDAL + LLK AYFYD QLQ+EI
Sbjct: 1   MTVTEREEEGNSSNTYIVEIKNEPVEFVLLQQEKQEEIDALSSVLLKGAYFYDDQLQIEI 60

Query: 61  E-----EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDF 120
           E     EESERSTKRIRTAESDE +SSSIKNTA+L  NQ ELG+DPLE++EEIVLKY DF
Sbjct: 61  EKEEDAEESERSTKRIRTAESDEVESSSIKNTASLEENQPELGIDPLEVLEEIVLKYHDF 120

Query: 121 INEIYQMLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWRDILDRGNLLVETLNRSLKTVA 180
           INEIYQML DEQ                KKVKIQWQKWR ILD+GNL+VE L RSLKTV 
Sbjct: 121 INEIYQMLKDEQ----------------KKVKIQWQKWRYILDKGNLIVEDLTRSLKTVE 180

Query: 181 IEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDEVLTMCL 240
           IEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFK VSDMKK DEVL MCL
Sbjct: 181 IEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKVVSDMKKRDEVLKMCL 240

Query: 241 DELETSKEELAEMVEVIQELKELDLEDDEAVEDGEAVGEEEEIGVEEFEKFN 287
           DELETS+EELAEM EVIQELKELDL+DDEAVED    GEEEEI V+  +K N
Sbjct: 241 DELETSQEELAEMFEVIQELKELDLQDDEAVED----GEEEEIKVDALQKIN 272

BLAST of PI0027942 vs. NCBI nr
Match: XP_038881500.1 (interaptin-like [Benincasa hispida])

HSP 1 Score: 284.6 bits (727), Expect = 9.8e-73
Identity = 181/307 (58.96%), Postives = 228/307 (74.27%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           MTVTE+ E+ NSSN K+VEIKKE VE   +++E++DID  LN L+K +   DHQL +E +
Sbjct: 1   MTVTEKSERANSSNTKVVEIKKEQVE-GGEEEEEKDIDLWLNELVKDSDLNDHQLPIEKD 60

Query: 61  -----------EESERSTKRIRTAESDEAQSSSIKNT-ANLGANQSELGMDPLELIEEIV 120
                      EE ERSTKR RT E      S +KNT AN  A++SE G+DPLE+ EEIV
Sbjct: 61  LINEEEDEEDAEELERSTKRTRTTE------SMMKNTAANAEADESEWGIDPLEITEEIV 120

Query: 121 LKYQDFINEIYQMLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWRDILDRGNLLVETLNR 180
           LKY+DFI++I+QML DEQK+   DK+K+   DE  + KIQWQKW +IL+R  +LVETLNR
Sbjct: 121 LKYRDFIHDIFQMLKDEQKE---DKEKE---DEENEAKIQWQKWSEILERAKVLVETLNR 180

Query: 181 SLKTVAIEMEWMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDE 240
            L T+A+EMEWMKSIDE  +EY++R+THVPDLLALLRDIN  IESS +F+ VSDMK  DE
Sbjct: 181 GLGTLALEMEWMKSIDEFEKEYTLRETHVPDLLALLRDINTGIESSTHFRLVSDMKNRDE 240

Query: 241 VLTMCLDELETSKEELAEMVEVIQELKELDLED-DEAVEDGEAVGEEE---EIGVEEFEK 292
           VL+MCLDELE S EEL EMVEVIQELKEL+L+D +E  EDGE  GE+E   E+  +E+E+
Sbjct: 241 VLSMCLDELERSNEELTEMVEVIQELKELELQDVEEDGEDGED-GEDELVLELEDDEYEE 293

BLAST of PI0027942 vs. NCBI nr
Match: XP_038902312.1 (myelin transcription factor 1-like [Benincasa hispida])

HSP 1 Score: 189.5 bits (480), Expect = 4.3e-44
Identity = 125/277 (45.13%), Postives = 178/277 (64.26%), Query Frame = 0

Query: 1   MTVTEREEKRNSSNRKIVEIKKEPVEFEHQQQEQEDIDALLNALLKSAYFYDHQLQLEIE 60
           MTV EREE+ NSSN K+ E KK+ VE     +E+EDI+  + A          ++  E +
Sbjct: 1   MTVREREERGNSSNTKVAENKKQNVE-----EEEEDINLQIGASNSQNQIKKVRITEESK 60

Query: 61  --EESERSTKRIRTAESDEAQSSSIKNTANLGANQSELGMDPLELIEEIVLKYQDFINEI 120
             EESERS+KR R  ES+EA+S+   N     ++ SE+G DPL   EEIV++Y DFI+ I
Sbjct: 61  DGEESERSSKRSRIKESEEAESTI--NEEEEESDSSEVGKDPLLATEEIVMEYHDFIDYI 120

Query: 121 YQMLTDEQKDKQKDKQKDEQTDEHKKVKIQWQKWRDILDRGNLLVETLNRSLKTVAIEME 180
           Y                      H+ +K   QKW +I++RG LLV+T+NRSL+TV +EME
Sbjct: 121 Y----------------------HQILKEDEQKWSEIVERGKLLVKTVNRSLRTVKLEME 180

Query: 181 WMKSIDELHREYSIRKTHVPDLLALLRDINERIESSPNFKAVSDMKKGDEVLTMCLDELE 240
            ++++++  ++Y  RKTH+P +L+LLR IN RIESS NF+ VS++K   +VL MCL ELE
Sbjct: 181 CIEAVEDFEKQYLFRKTHIPQILSLLRHINSRIESSTNFRLVSNIKNRRKVLPMCLRELE 240

Query: 241 TSKEELAEMVEVIQELKELDLEDDEAVEDGEAVGEEE 276
           TSK+EL+EM++VIQ LKELDL+ ++  +D +   EEE
Sbjct: 241 TSKQELSEMIDVIQVLKELDLKSEDDDDDADDDDEEE 248