PI0027772 (gene) Melon (PI 482460) v1

Overview
NamePI0027772
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionVacuolar-processing enzyme
Locationchr02: 16269798 .. 16274528 (-)
RNA-Seq ExpressionPI0027772
SyntenyPI0027772
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAACTTGTGATAGAAACCCAACAAAGCTATTAATCATTCTCATAACCTTACTGTGTTTCAACAACATTATAGCTAAGAATGAGTGGATTAGTGATGCCACTACTGACGAAACTGGGAAGAGATGGGCACTTCTGATTGCTGGCTCCAATGGCTATGATAATTACAGGCACCAGGTACCCTTCTAGTATCATTCCAATTACACAAACTGTGCAGAAAATTATTTGATAAAGTCACCACAATATAGTTCACAATTAAGAAGCATAAAAAACTTTAATTTGACTCAAGTGTCTATGACGAACACATGAAAACTCTGACACCTATTGAACATGTATCAGCATACACTTATGAGTGAAATATATGTGTTAGACACTAGTTCTACAAAGTCATGTAAGGTCCAACATTTGCTAAACATCCATAATATTTGTATCCACCAAACTAATTTTTTAAGCAAATAAATGCAACTCTTCCACTTTGAACTTCTTTCTAGCATAGAAATGATATGTATTTTAATAAATGTGTTCTCATGTTTGTGTCATAAATCTAAAGAAAATGACATGTCACCGTGTCTTGTATCCATATTTGTGTCATAAATCTAAAGAAAATGACATGTCACCGTGTCTTGTATCCATATTTGTGCTTCTTAGGTTTACATTATATTTATCCATTTAGTGTTGCAGTCTCATTCAATAATTTTTTCAACCATAGTCACTATCTTCTTTTTGATATAAAAACCAAAAACCTAGGCGGATGTATGCCACGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATCATAGTTTTCATGTACGATGATATTGCATTTCATCCAAGCAATCCAAGGCCAGGAATCATTATCAACAAACCAGATGGGGATGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAAAGCATGTGAATGCAGTCAACTTCTACGCTGCCATTTTGGGCAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTTATTCACAGTGGCCCTCATGATCATATTTTCATCTACTACTCCGACCATGGTGCTGCTGGGATGCTTGGTAATAAGTTTACCCTTGCAATTCCTTTTTGCCTCAACAAATTAAGGGTCTGTTTGGATTGACTCTCTAAATGCTTAGAAAAGACAACTCAAACAGGTCCACCTTGAACTCCCTTATTAAATTTGACAGTATTTTGTTCCACATGATCATAGGAATGCCCGACGGCGATTATATATTCGTCAACGATCTAATAGATGTGCTGAAAAGGAAACACAAAGCCAAGAGTTACAAAAGCATGGTACTAAAACATGGAATATAGATCATATAACAATAATTACAAAGTTCAAATTATATATTAAAGTTACAATTAGTTAACATAAAAATTCAGGTAATATATGTGGAAGCATGCGAGTCGGGGAGTATGTTTGAAGGAGTACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAAGAGTCTCATCGTCCTCCTCCCAAAGACTATAACACTTGTCTTGGAGATTTGTTCAGCGTCGCTTGGATGGAAGACAGGCAATATTTTCTTTCAACACCTTTTATCTATGTTAAAGAATTGATATAATTAAATTAACTCATCCCTTTAAGCTTTGGAGTTCGGTTGTATTTTAACATAGTATGCATCCGAGTTGGAGGTCCTATATTCAGGCCCTTGTAACGCCGTTTTCAAGCCTACATAGTCACTCTCCAATCATCCTAAATGAATCTATGTTGTGACATAACGTGCAGTGACAAGCATGATTTGAGCAAAGAAACTTTGAACCAACAATATCATACGGTAACCTTCCTAATTCTTAAATGAACTCAATCTTCTTAAATAGCACTACTAATAAACGTTTCCTTGTACATTCCCAAACTGCAGGTTCGAAGAAGAACTTTTGCTGATAAGTCTGGCTACGGTTCTCATGTAATGTTGTATGGGAACAAAAGGATCGGCAATGATTTCCTTAACACCTTTCTTGGTAATAGCCCTGATTATGACAACTATAAATCCTCTGTTCAGAGTACTCAAACCATTTCTTTGCCATCAAAATTGTCATCAAATGCGGTAAGCCAGCGCGATGCTAGTCTCATTCATTATCAGTACAAGGTATGATTTGTCCAACAACGTATATATTCAATTATGTTCCAATCTACTACATAGTCTAAGTGTGTAGACTTTTGCTTTGATCAGTTTCAAGAAGCTCCATTTGGGTCAAGAGAAAAGATGGAGGCTAGAGAGCAGTTAGAGGATGAAATTTTCAAAAGAAGGCATGCTGACAACAGTATAAATAACATTGGTAAACTTCTGTTTGGACAAGCAAGGAGCTCAGAAATACTTCACAATGTTAGGGCGCAAGGGCAATCCCTGGTTGATGATTGGCAATGCTTCAAGACATTTGTGAGTCCTATTTTTCAAGTCCAAACTTATTTTATACCATCAGAAACTTTGTTAATGCAACAATCTCTATAATACAGGTAAAAATCTATGAGAAACATTGCAACAGAATGTCAAGCTATGGGATGAAATACACACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCCTGTCTCCAAACTTGCCTTGGTAAAACCTGAAGTGGGTTTAATTTCTAATATTGTTAACCTGCCAGTCTAGGCACCCAGTGTTTATGTATGTATACAGAACTTGTAATGCAAATAATAAACTAATAATATTAATCTTGAGCTGGAACCATCGATCATTTCGAACTTTCTTTGTTAATACACAAGCAAACTATCTACCGAAAAATCTTATTTCAACCCCAGACAACAAATGCATTTCTAGCTCATTCAGAGGGAAAGAATCCGTATAAAGTTTGCTTTGGATCATTATGGCAATGTGGTTGCATAAGGTTTAATCAGACTTTATGAACAAAAAATTCTAAGTGGACATAATTAAACAAATCAATAGATCTTTTTCAGCAAACAACAAGAAATTAAGCATAACAATTTCAACCACTGTGTTTTAATTCTAAATACGAAATATTAGCAATAATGTTAACGTAAGGAATTTAGTCGGAGAAGAAACGGGGTACTTCAAAGAGAACGACCTTTTTACCGGCGGCGAGGGAGTGTACAGATTCTTGTTGGAGCTTATAGTCCCCGTCGAAGTTAGCTAATGCCCCGTCCGGCAACGTATCCCCAACGGCAACCGGAGCCATTTGGCGTTGATTTGGATCCAGCCGGCGGCAACTACAAAGTGGGAAGGGGTTTTTGTACGGAAGGCCTCCATTGGAGCCCAAATGAAATGTGACCGCCGATGCAAATTCTAACGAGTAATTGTGTTAGGATCACCTCAGCCCCTTGCCAATTTATCAAAATAACCCTTCCTGCGCGCAAGGGTAGTTTGCTATGAATTTCTTCTCATGCCTATTTCTCGGCTCTCGTTCTCGTACTTCGTTGATCGTTATCGCCTAACGTAATATCCAAATTTCTCATCACTCGTCTTTTTGTCGCTCATGAATAACTTCTTGTTCCTCTTTCTCGTTCAAAATACCTATTTCTCGTTCTGATTCTCATGCCTAAATACTATTTCTCGCTCGTGAATAACTTCTCGTTCCTCTTTCTCGTTTTTCTTTATCGTTCAAGTACCGATTTCTCGTTTCTTTGTTCAGATTCTTCTTTCCCTTTCTAGTTTCTAAGAAAATTTTGGAGAATAGAGAAGAAGAAAGAAAGCATAAAAATGGAAGAAGAAGATGACACAAAAAAAAAAAAAAACGAAAAAGAAAAAGAAAAAGAAAAAAATAAAGAAGAAAACGCAAAAAAAAAAGAACAAACGAGAAAAAAAAAAAAGAAACAAACGAGAAAAAAAAAACTATTGATGAGGAAGAAAGAAAGAAAAAAGAGTTAAAAGTGTAATTTCATAGGATAATGACATCAAGCCAAACCTCATTTGTTTTTTAAACTTTGGACTATTTTTCATTTGGGCCTTTAAAAATAGCTATCTATACAATTATTCCTAGTGGAAACTATGGGATTAAAAAGAGAAATTATAAGGGGGGTTTTGTACGAACAACCTCCAAATGGAGGCCCAAATAAAATTTGACCCACGATTCAAATTCTAACGAGTAATGACCTTATGATGATGTCAGTCGCCTATGAATTTACCAAAGTAACCTCATCTTCATGCGATGCCATTCTACTACAAACTTCTTATCGTTTCTATTTCTCACTTCTCATTCTCGTTGTTTGCTACTCGTTCTCACTCAACGTAATACCCAAATTTTCCACCACTTGCCTTTTTCTCGCTCTTGAACAATCTTTCGTTTCTCTTTCTCGTTTCAAGTACCTATTTCTTGCTTTTGATTTTCATGCCTAAATAAATTTCTCACTTGTGAATAACTTCTCATTTCTCTTTCTTGTTCCAATTGCCTATTTCTCGTTTCACTTTGATTTTTTTTCTTTGTTCAAAATCTTTTTTCTCTTGCTAGCTTCTGCAGAACGAAGAAAAATTTGGAGAACGAAGAAGAAGAAGAGAAGCGAGAAGAAGAAAAAAAAGCATAAAAATGGAAGAAGAAGAAGATGACAACGCAAGAAAACGAAAAAATGAAAAAAAAAAAGAAAGAAGGAAAAAAACGACTGCAAAATGGAGAAGGAATAAGGAAGAAGTGGTTAAAGTATAA

mRNA sequence

ATGACAACTTGTGATAGAAACCCAACAAAGCTATTAATCATTCTCATAACCTTACTGTGTTTCAACAACATTATAGCTAAGAATGAGTGGATTAGTGATGCCACTACTGACGAAACTGGGAAGAGATGGGCACTTCTGATTGCTGGCTCCAATGGCTATGATAATTACAGGCACCAGGCGGATGTATGCCACGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATCATAGTTTTCATGTACGATGATATTGCATTTCATCCAAGCAATCCAAGGCCAGGAATCATTATCAACAAACCAGATGGGGATGATCATGTGAATGCAGTCAACTTCTACGCTGCCATTTTGGGCAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTTATTCACAGTGGCCCTCATGATCATATTTTCATCTACTACTCCGACCATGGTGCTGCTGGGATGCTTGGAATGCCCGACGGCGATTATATATTCGTCAACGATCTAATAGATGTGCTGAAAAGGAAACACAAAGCCAAGAGTTACAAAAGCATGGTAATATATGTGGAAGCATGCGAGTCGGGGAGTATGTTTGAAGGAGTACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAAGAGTCTCATCGTCCTCCTCCCAAAGACTATAACACTTGTCTTGGAGATTTGTTCAGCGTCGCTTGGATGGAAGACAGTGACAAGCATGATTTGAGCAAAGAAACTTTGAACCAACAATATCATACGGTTCGAAGAAGAACTTTTGCTGATAAGTCTGGCTACGGTTCTCATGTAATGTTGTATGGGAACAAAAGGATCGGCAATGATTTCCTTAACACCTTTCTTGGTAATAGCCCTGATTATGACAACTATAAATCCTCTGTTCAGAGTACTCAAACCATTTCTTTGCCATCAAAATTGTCATCAAATGCGGTAAGCCAGCGCGATGCTAGTCTCATTCATTATCAGTACAAGTTTCAAGAAGCTCCATTTGGGTCAAGAGAAAAGATGGAGGCTAGAGAGCAGTTAGAGGATGAAATTTTCAAAAGAAGGCATGCTGACAACAGTATAAATAACATTGGTAAACTTCTGTTTGGACAAGCAAGGAGCTCAGAAATACTTCACAATGTTAGGGCGCAAGGGCAATCCCTGGTTGATGATTGGCAATGCTTCAAGACATTTGTAAAAATCTATGAGAAACATTGCAACAGAATGTCAAGCTATGGGATGAAATACACACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCCTGTCTCCAAACTTGCCTTGAACGAAGAAGAAGAAGAGAAGCGAGAAGAAGAAAAAAAAGCATAAAAATGGAAGAAGAAGAAGATGACAACGCAAGAAAACGAAAAAATGAAAAAAAAAAAGAAAGAAGGAAAAAAACGACTGCAAAATGGAGAAGGAATAAGGAAGAAGTGGTTAAAGTATAA

Coding sequence (CDS)

ATGACAACTTGTGATAGAAACCCAACAAAGCTATTAATCATTCTCATAACCTTACTGTGTTTCAACAACATTATAGCTAAGAATGAGTGGATTAGTGATGCCACTACTGACGAAACTGGGAAGAGATGGGCACTTCTGATTGCTGGCTCCAATGGCTATGATAATTACAGGCACCAGGCGGATGTATGCCACGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATCATAGTTTTCATGTACGATGATATTGCATTTCATCCAAGCAATCCAAGGCCAGGAATCATTATCAACAAACCAGATGGGGATGATCATGTGAATGCAGTCAACTTCTACGCTGCCATTTTGGGCAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTTATTCACAGTGGCCCTCATGATCATATTTTCATCTACTACTCCGACCATGGTGCTGCTGGGATGCTTGGAATGCCCGACGGCGATTATATATTCGTCAACGATCTAATAGATGTGCTGAAAAGGAAACACAAAGCCAAGAGTTACAAAAGCATGGTAATATATGTGGAAGCATGCGAGTCGGGGAGTATGTTTGAAGGAGTACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAAGAGTCTCATCGTCCTCCTCCCAAAGACTATAACACTTGTCTTGGAGATTTGTTCAGCGTCGCTTGGATGGAAGACAGTGACAAGCATGATTTGAGCAAAGAAACTTTGAACCAACAATATCATACGGTTCGAAGAAGAACTTTTGCTGATAAGTCTGGCTACGGTTCTCATGTAATGTTGTATGGGAACAAAAGGATCGGCAATGATTTCCTTAACACCTTTCTTGGTAATAGCCCTGATTATGACAACTATAAATCCTCTGTTCAGAGTACTCAAACCATTTCTTTGCCATCAAAATTGTCATCAAATGCGGTAAGCCAGCGCGATGCTAGTCTCATTCATTATCAGTACAAGTTTCAAGAAGCTCCATTTGGGTCAAGAGAAAAGATGGAGGCTAGAGAGCAGTTAGAGGATGAAATTTTCAAAAGAAGGCATGCTGACAACAGTATAAATAACATTGGTAAACTTCTGTTTGGACAAGCAAGGAGCTCAGAAATACTTCACAATGTTAGGGCGCAAGGGCAATCCCTGGTTGATGATTGGCAATGCTTCAAGACATTTGTAAAAATCTATGAGAAACATTGCAACAGAATGTCAAGCTATGGGATGAAATACACACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCCTGTCTCCAAACTTGCCTTGAACGAAGAAGAAGAAGAGAAGCGAGAAGAAGAAAAAAAAGCATAAAAATGGAAGAAGAAGAAGATGACAACGCAAGAAAACGAAAAAATGAAAAAAAAAAAGAAAGAAGGAAAAAAACGACTGCAAAATGGAGAAGGAATAAGGAAGAAGTGGTTAAAGTATAA

Protein sequence

MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDDHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLERRRRREARRRKKSIKMEEEEDDNARKRKNEKKKERRKKTTAKWRRNKEEVVKV
Homology
BLAST of PI0027772 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 4.7e-139
Identity = 245/431 (56.84%), Postives = 313/431 (72.62%), Query Frame = 0

Query: 37  DETGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D  G RWA+L+AGSNGY NYRHQ+DVCHAYQ+L+KGG  +ENIIVFMYDDIA +  NPRP
Sbjct: 51  DFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRP 110

Query: 97  GIIINKPDGDD------------HVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIY 156
           G+IINKPDGDD             V+A NFYAA+LGNKSALTGGSGKV+ SGP+DHIF+Y
Sbjct: 111 GVIINKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVY 170

Query: 157 YSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENI 216
           Y+DHG  G+LGMP G Y++ +DL +VLK+KH + +YKS+V Y+EACESGS+FEG+LP+++
Sbjct: 171 YTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDL 230

Query: 217 KIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQ 276
            IYATTASNA E SW  YCP +   PPP +Y+TCLGDL+S+AWMEDS+ H+L  E+L QQ
Sbjct: 231 NIYATTASNAEESSWGYYCPGDK-PPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQ 290

Query: 277 YHTVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSK 336
           Y  V+ RT ++   YGSHVM YG+  +  + L  +LG +P  DN  S V  T+  SL  +
Sbjct: 291 YKLVKNRTISEP--YGSHVMEYGDIGLSKNDLYQYLGTNPANDN-NSFVDETEN-SLKLR 350

Query: 337 LSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFG 396
             S AV+QRDA LIH+  KF++AP GS +K EA +Q+ + +  R+H DNS+  IG+LLFG
Sbjct: 351 TPSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFG 410

Query: 397 QARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITM 456
             + +E+L  VR  G  LVD+W C KT VK +E HC  +S YGMK+ R+ ANICNAGI  
Sbjct: 411 IEKGTELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPN 470

BLAST of PI0027772 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.3e-138
Identity = 242/429 (56.41%), Postives = 311/429 (72.49%), Query Frame = 0

Query: 40  GKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGII 99
           G RWA+L AGS+GY NYRHQAD+CHAYQ+L+KGG+ DENIIVFMYDDIAF+  NPR G+I
Sbjct: 47  GTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVI 106

Query: 100 INKPDGD------------DHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSD 159
           IN P+GD            + V A NFYAA+LG+KS LTGGSGKV++SGP+DHIFI+YSD
Sbjct: 107 INSPNGDEVYKGVPKDYTGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSD 166

Query: 160 HGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIY 219
           HG  G+LG P G YI+ +DL +VLK+KH + +YK++V Y+EACESGS+FEG+LPE+I +Y
Sbjct: 167 HGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVY 226

Query: 220 ATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHT 279
           ATTASNA E SW TYCP E   PPP +Y+TCLGDL+SVAWMEDSD+H+L  ETL+QQY  
Sbjct: 227 ATTASNADESSWGTYCPGEDPSPPP-EYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKL 286

Query: 280 VRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSS 339
           V+ RT +    YGSHVM YG+  +  D L  +LG  P  +N    +      SL S  SS
Sbjct: 287 VKERTISGGLYYGSHVMQYGDVGLSKDILFHYLGTDPANEN----LTFVDENSLWS--SS 346

Query: 340 NAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQAR 399
            AV+QRDA L+H+  KF++AP GS +K EAR+Q+ + +  R H D+S+  +GKLLFG  +
Sbjct: 347 KAVNQRDADLVHFWDKFRKAPEGSPKKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEK 406

Query: 400 SSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQM 457
           + E+L+ VR  G +LVDDW C KT V+ +E HC  +S YGMK+ R+ AN+CN GI   QM
Sbjct: 407 APELLNAVRPAGSALVDDWDCLKTMVRTFETHCGSLSQYGMKHMRSFANMCNVGIKKEQM 466

BLAST of PI0027772 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.1e-137
Identity = 241/431 (55.92%), Postives = 309/431 (71.69%), Query Frame = 0

Query: 37  DETGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D  G RWA+L+AGSNG+ NYRHQAD+CHAYQ+L+KGG+ DENIIVFMYDDIAF+  NPRP
Sbjct: 55  DSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRP 114

Query: 97  GIIINKPDGDD------------HVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIY 156
           G+IIN P GDD             V    F+A +LGNK+ALTGGSGKV+ SGP+DHIFI+
Sbjct: 115 GVIINHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIF 174

Query: 157 YSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENI 216
           YSDHG  G+LGMP   YI+ ++LIDVLK+KH + +YKS+V Y+EACESGS+FEG+L E +
Sbjct: 175 YSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGL 234

Query: 217 KIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQ 276
            IYATTASNA E SW TYCP E   PPP +Y+TCLGDL+S+AWMEDSD H+L  ETL+QQ
Sbjct: 235 NIYATTASNAEESSWGTYCPGEIPGPPP-EYSTCLGDLYSIAWMEDSDIHNLRTETLHQQ 294

Query: 277 YHTVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSK 336
           Y  V+ RT A  + YGSHVM YG+  +  + L T+LG +P  DNY    +++       +
Sbjct: 295 YELVKTRT-ASYNSYGSHVMQYGDIGLSKNNLFTYLGTNPANDNYTFVDENS------LR 354

Query: 337 LSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFG 396
            +S AV+QRDA L+H+  K+++AP G+  K EA++Q  + +  R H D+SI  IGKLLFG
Sbjct: 355 PASKAVNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFG 414

Query: 397 QARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITM 456
             +  EIL+ VR  GQ LVDDW C K+ V+ +E HC  +S YGMK+ R++ANICN GI  
Sbjct: 415 IEKGPEILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGK 474

BLAST of PI0027772 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 489.2 bits (1258), Expect = 5.7e-137
Identity = 235/429 (54.78%), Postives = 307/429 (71.56%), Query Frame = 0

Query: 39  TGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGI 98
           +G RWA+L+AGS+GY NYRHQAD+CHAYQ+L+KGG+ +ENI+VFMYDDIA +  NPRPG 
Sbjct: 56  SGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGT 115

Query: 99  IINKPDG------------DDHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYS 158
           IIN P G             D VN  N +A ILG+K+A+ GGSGKV+ SGP+DHIFI+YS
Sbjct: 116 IINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYS 175

Query: 159 DHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKI 218
           DHG  G+LGMP   Y++ NDL DVLK+KH   +YKS+V Y+EACESGS+FEG+LPE + I
Sbjct: 176 DHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNI 235

Query: 219 YATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYH 278
           YATTASNA E SW TYCP E   PPP +Y TCLGDL+SVAWMEDS  H+L  ETL+QQY 
Sbjct: 236 YATTASNAEESSWGTYCPGEEPSPPP-EYETCLGDLYSVAWMEDSGMHNLQTETLHQQYE 295

Query: 279 TVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLS 338
            V+RRT      YGSHVM YG+  I  D L+ ++G +P  DN+  +     ++  PS+++
Sbjct: 296 LVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNF--TFADANSLKPPSRVT 355

Query: 339 SNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQA 398
               +QRDA L+H+  K+++AP GS  K EA++Q+ + +  R H DNS+  +GK+LFG +
Sbjct: 356 ----NQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGIS 415

Query: 399 RSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQ 456
           R  E+L+ VR+ GQ LVDDW C K  V+ +E+HC  +S YG+K+ R+ ANICNAGI M Q
Sbjct: 416 RGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQ 475

BLAST of PI0027772 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 481.1 bits (1237), Expect = 1.6e-134
Identity = 232/431 (53.83%), Postives = 310/431 (71.93%), Query Frame = 0

Query: 37  DETGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D+   +WA+L+AGS+GY NYRHQADVCHAYQ+LKKGG+ +ENI+VFMYDDIA +  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 97  GIIINKPDGD------------DHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIY 156
           G+IIN P+G+            D VN  N  A ILGNK+AL GGSGKV+ SGP+DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 157 YSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENI 216
           YSDHG  G+LGMP    ++ NDL DVLK+K+ + +YKS+V Y+EACESGS+FEG+LPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 217 KIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQ 276
            IYATTASNA E SW TYCP E    PP +Y TCLGDL+SVAW+EDS+KH+L  ETL++Q
Sbjct: 219 NIYATTASNAEESSWGTYCPGED-PSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQ 278

Query: 277 YHTVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSK 336
           Y  V++RT      YGSHVM +G+  +  + L  F+G +P  +N+  +  +  +I  PS+
Sbjct: 279 YELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENF--TFVNENSIRPPSR 338

Query: 337 LSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFG 396
           ++    +QRDA L+H+ +K+Q+AP GS  K+EA++Q+ + +  R H DNSI  IG LLFG
Sbjct: 339 VT----NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG 398

Query: 397 QARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITM 456
                 +L+ VR  G+ LVDDW C K+ V+ +E+HC  +S YG+K+ R+IAN+CNAGI M
Sbjct: 399 -LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQM 458

BLAST of PI0027772 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.9e-244
Identity = 424/468 (90.60%), Postives = 432/468 (92.31%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILIT LCFNNIIAKNEWISDATTD+TGK WALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDG+D            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYYSDHGAAGMLGMP+GDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           DVLKRKHKAK+YKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPK+SH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
           R  PKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYH VRRRTF DKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAP 360
           KRIGNDFL+TFLGNSPDYDNYK SVQS QTIS  SKLSSN+VSQRDASLIHYQYKFQEAP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQC 420
           FGSREKMEAREQLEDEIFKRRHADNSIN+IGKLLFGQARSSEILHNVRAQGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 455

BLAST of PI0027772 vs. ExPASy TrEMBL
Match: A0A5D3DZP1 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00260 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 9.5e-236
Identity = 414/468 (88.46%), Postives = 422/468 (90.17%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILIT LCFNNIIAKNEWISDATTD+TGK WALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDG+D            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYYSDHGAAGMLGMP+GDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           DVLKRKHKAK+YKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPK+SH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
           R  PKDYNTCLGD          DKHDLSKETLNQQYH VRRRTF DKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDF---------DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAP 360
           KRIGNDFL+TFLGNSPDYDNYK SVQS QTIS  SKLSSN+VSQRDASLIHYQYKFQEAP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQC 420
           FGSREKMEAREQLEDEIFKRRHADNSIN+IGKLLFGQARSSEILHNVRAQGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 446

BLAST of PI0027772 vs. ExPASy TrEMBL
Match: A0A1S3CJ43 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo OX=3656 GN=LOC103501567 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.9e-205
Identity = 357/386 (92.49%), Postives = 364/386 (94.30%), Query Frame = 0

Query: 83  MYDDIAFHPSNPRPGIIINKPDGDD------------HVNAVNFYAAILGNKSALTGGSG 142
           MYDDIAFHPSNPRPGIIINKPDG+D            HVNAVNFYAAILGNKSALTGGSG
Sbjct: 1   MYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKHVNAVNFYAAILGNKSALTGGSG 60

Query: 143 KVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEAC 202
           KVIHSGP DHIFIYYSDHGAAGMLGMP+GDYIFVNDLIDVLKRKHKAK+YKSMVIYVEAC
Sbjct: 61  KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC 120

Query: 203 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMED 262
           ESGSMFEGVLPENIKIYATTASNATEDSWATYCPK+SHR  PKDYNTCLGDLFSVAWMED
Sbjct: 121 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHR-SPKDYNTCLGDLFSVAWMED 180

Query: 263 SDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYK 322
           SDKHDLSKETLNQQYH VRRRTF DKSGYGSHVMLYGNKRIGNDFL+TFLGNSPDYDNYK
Sbjct: 181 SDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYK 240

Query: 323 SSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRH 382
            SVQS QTIS  SKLSSN+VSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRH
Sbjct: 241 YSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRH 300

Query: 383 ADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKY 442
           ADNSIN+IGKLLFGQARSSEILHNVRAQGQSLVDDW CFKTFVKIYEKHCNRMSSYGMKY
Sbjct: 301 ADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKY 360

Query: 443 TRAIANICNAGITMHQMDQACLQTCL 457
           TRAIANICNAGITMHQMDQACLQTCL
Sbjct: 361 TRAIANICNAGITMHQMDQACLQTCL 385

BLAST of PI0027772 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 5.8e-201
Identity = 345/469 (73.56%), Postives = 400/469 (85.29%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           M TC    TK  ++LI LL F++IIAK E +  A+TD+ GKRWA+L+AGS+GY+NYRHQA
Sbjct: 1   MATCYATSTK-FVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
           DVCHAYQIL+KGG+PDENIIVFMYDDIAF+PSNPRPG++INKPDG D            H
Sbjct: 61  DVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VN++NFYA ILGN+SALTGGSGKV+ S  HDHIFIYY+DHG+AG+LGMP+GDY++  DL+
Sbjct: 121 VNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLM 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           +VLK+KH+AKSYKSMVIYVEACESGSM EG+LPENIKIYATTASNATE+SWATYCP +  
Sbjct: 181 EVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQ-F 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
             PP DY+TCLGDL+S+AWMEDSDKHDLSKETL QQY  VRRRT  DK GYGSHVMLYGN
Sbjct: 241 PSPPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSK-LSSNAVSQRDASLIHYQYKFQEA 360
           K IGN+ L+T++G +PD  NY SSVQST TI+ PSK L SNAVSQRDASLIHY +KFQ+A
Sbjct: 301 KSIGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKA 360

Query: 361 PFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQ 420
           PFGSREK EAR+QLEDEI  RRH D+SI +I KLLFGQA+SSE+L+NVR QGQSLVDDW 
Sbjct: 361 PFGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWG 420

Query: 421 CFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           CFK FVK YEKHC R+S YGMKYTRA+ANICNAGIT++QMDQACL+TCL
Sbjct: 421 CFKKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCL 467

BLAST of PI0027772 vs. ExPASy TrEMBL
Match: A0A6J1JF37 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111485183 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.3e-200
Identity = 354/468 (75.64%), Postives = 388/468 (82.91%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           MTT    PT LL  LI LL FN+IIAK EW+S A TD+ GKRWALL+AGSNGYDNYRHQA
Sbjct: 1   MTTFATTPTTLL--LIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
           DVCHAYQIL+K GIPDENIIVFMYDDIAFHP NPRPGIIINKP G D             
Sbjct: 61  DVCHAYQILRK-GIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKD 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VN+VNFYAAILGNKS +TGGSGKVI SGP+DHIFIYY+DHGAAGMLGMP GDYI+ NDL+
Sbjct: 121 VNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLM 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           DVLKRKH+AKSYKSMVIYVEACESGSMFEG+LPEN+ IYATTASNATEDSWA YCP +S 
Sbjct: 181 DVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQS- 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
             P   Y+TCLGDL+SVAWMEDSDKHD SKETLNQQYHTVRRRT  DK GYGSHVMLYGN
Sbjct: 241 PTPHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAP 360
           +  GN+FLNT+ GN+ D +N +SSV+ST  IS      SNAVSQRDASL+HY YKFQ AP
Sbjct: 301 ESFGNNFLNTYFGNTSDDNNNESSVKSTHIIS----SLSNAVSQRDASLLHYWYKFQNAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQC 420
           +GSREKMEA EQLEDEI  RRHADNSIN IG LLFG+A+SS +L+  R  GQS VDDW C
Sbjct: 361 YGSREKMEASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           FK +VKIYEKHC RMS YGMKYT+A+AN CNAGITM QMDQACLQTC+
Sbjct: 421 FKNYVKIYEKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCV 460

BLAST of PI0027772 vs. NCBI nr
Match: KAA0046547.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 854.7 bits (2207), Expect = 3.9e-244
Identity = 424/468 (90.60%), Postives = 432/468 (92.31%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILIT LCFNNIIAKNEWISDATTD+TGK WALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDG+D            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYYSDHGAAGMLGMP+GDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           DVLKRKHKAK+YKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPK+SH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
           R  PKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYH VRRRTF DKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAP 360
           KRIGNDFL+TFLGNSPDYDNYK SVQS QTIS  SKLSSN+VSQRDASLIHYQYKFQEAP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQC 420
           FGSREKMEAREQLEDEIFKRRHADNSIN+IGKLLFGQARSSEILHNVRAQGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 455

BLAST of PI0027772 vs. NCBI nr
Match: XP_038880044.1 (vacuolar-processing enzyme-like [Benincasa hispida])

HSP 1 Score: 838.2 bits (2164), Expect = 3.8e-239
Identity = 405/468 (86.54%), Postives = 427/468 (91.24%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           MTTC+R+PTKLL+ILI  + FNNIIAK EWISDAT ++TGKRWALL+AGSNGY+NYRHQA
Sbjct: 1   MTTCNRSPTKLLVILIIFVFFNNIIAKKEWISDATANQTGKRWALLVAGSNGYENYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
           DVCHAYQILKKGGIPDENIIVFMYDDIAFHP+NPR GIIINKP G D             
Sbjct: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPNNPRQGIIINKPGGPDVYQGVPKDYTGKQ 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VNAVNFYAA+ GNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMP+ DY+FVNDLI
Sbjct: 121 VNAVNFYAALSGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPEDDYVFVNDLI 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           D LKRKHKAKSYKSMVIYVEACESGSMFEG+LPENIKIYATTASNATEDSWATYCPKES 
Sbjct: 181 DALKRKHKAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATEDSWATYCPKESR 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
            PPPKDYNTCLGDL+SVAWMEDSDKHDLSKETLNQQYH VRRRT  DKSG GSHVMLYGN
Sbjct: 241 GPPPKDYNTCLGDLYSVAWMEDSDKHDLSKETLNQQYHAVRRRTLVDKSGVGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAP 360
           +RIG DFLNTF+GNSPDY+NYKSSVQS  TIS PSKLSSN VSQRDASLI+YQYKFQ+AP
Sbjct: 301 RRIGTDFLNTFIGNSPDYNNYKSSVQSIHTISSPSKLSSNGVSQRDASLIYYQYKFQKAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQC 420
            GSREKMEAREQLEDEI KRRHADNSINNIGKLLFGQARSS++L+ VR QGQSLVDDW C
Sbjct: 361 IGSREKMEAREQLEDEILKRRHADNSINNIGKLLFGQARSSKVLNKVREQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           FKTFVKIYEKHC RMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL
Sbjct: 421 FKTFVKIYEKHCKRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 468

BLAST of PI0027772 vs. NCBI nr
Match: TYK28710.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 825.9 bits (2132), Expect = 2.0e-235
Identity = 414/468 (88.46%), Postives = 422/468 (90.17%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           MTTCDRNPTKLLIILIT LCFNNIIAKNEWISDATTD+TGK WALLIAGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
                      GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDG+D            H
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYYSDHGAAGMLGMP+GDYIFVNDLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           DVLKRKHKAK+YKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPK+SH
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
           R  PKDYNTCLGD          DKHDLSKETLNQQYH VRRRTF DKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDF---------DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAP 360
           KRIGNDFL+TFLGNSPDYDNYK SVQS QTIS  SKLSSN+VSQRDASLIHYQYKFQEAP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQC 420
           FGSREKMEAREQLEDEIFKRRHADNSIN+IGKLLFGQARSSEILHNVRAQGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 446

BLAST of PI0027772 vs. NCBI nr
Match: XP_008463400.2 (PREDICTED: vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo])

HSP 1 Score: 724.2 bits (1868), Expect = 8.0e-205
Identity = 357/386 (92.49%), Postives = 364/386 (94.30%), Query Frame = 0

Query: 83  MYDDIAFHPSNPRPGIIINKPDGDD------------HVNAVNFYAAILGNKSALTGGSG 142
           MYDDIAFHPSNPRPGIIINKPDG+D            HVNAVNFYAAILGNKSALTGGSG
Sbjct: 1   MYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKHVNAVNFYAAILGNKSALTGGSG 60

Query: 143 KVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEAC 202
           KVIHSGP DHIFIYYSDHGAAGMLGMP+GDYIFVNDLIDVLKRKHKAK+YKSMVIYVEAC
Sbjct: 61  KVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLIDVLKRKHKAKNYKSMVIYVEAC 120

Query: 203 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMED 262
           ESGSMFEGVLPENIKIYATTASNATEDSWATYCPK+SHR  PKDYNTCLGDLFSVAWMED
Sbjct: 121 ESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHR-SPKDYNTCLGDLFSVAWMED 180

Query: 263 SDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYK 322
           SDKHDLSKETLNQQYH VRRRTF DKSGYGSHVMLYGNKRIGNDFL+TFLGNSPDYDNYK
Sbjct: 181 SDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKRIGNDFLDTFLGNSPDYDNYK 240

Query: 323 SSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRH 382
            SVQS QTIS  SKLSSN+VSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRH
Sbjct: 241 YSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRH 300

Query: 383 ADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKY 442
           ADNSIN+IGKLLFGQARSSEILHNVRAQGQSLVDDW CFKTFVKIYEKHCNRMSSYGMKY
Sbjct: 301 ADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFKTFVKIYEKHCNRMSSYGMKY 360

Query: 443 TRAIANICNAGITMHQMDQACLQTCL 457
           TRAIANICNAGITMHQMDQACLQTCL
Sbjct: 361 TRAIANICNAGITMHQMDQACLQTCL 385

BLAST of PI0027772 vs. NCBI nr
Match: XP_022148051.1 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 [Momordica charantia])

HSP 1 Score: 710.3 bits (1832), Expect = 1.2e-200
Identity = 345/469 (73.56%), Postives = 400/469 (85.29%), Query Frame = 0

Query: 1   MTTCDRNPTKLLIILITLLCFNNIIAKNEWISDATTDETGKRWALLIAGSNGYDNYRHQA 60
           M TC    TK  ++LI LL F++IIAK E +  A+TD+ GKRWA+L+AGS+GY+NYRHQA
Sbjct: 1   MATCYATSTK-FVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------H 120
           DVCHAYQIL+KGG+PDENIIVFMYDDIAF+PSNPRPG++INKPDG D            H
Sbjct: 61  DVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLI 180
           VN++NFYA ILGN+SALTGGSGKV+ S  HDHIFIYY+DHG+AG+LGMP+GDY++  DL+
Sbjct: 121 VNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLM 180

Query: 181 DVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESH 240
           +VLK+KH+AKSYKSMVIYVEACESGSM EG+LPENIKIYATTASNATE+SWATYCP +  
Sbjct: 181 EVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQ-F 240

Query: 241 RPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGN 300
             PP DY+TCLGDL+S+AWMEDSDKHDLSKETL QQY  VRRRT  DK GYGSHVMLYGN
Sbjct: 241 PSPPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGN 300

Query: 301 KRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSK-LSSNAVSQRDASLIHYQYKFQEA 360
           K IGN+ L+T++G +PD  NY SSVQST TI+ PSK L SNAVSQRDASLIHY +KFQ+A
Sbjct: 301 KSIGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKA 360

Query: 361 PFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQ 420
           PFGSREK EAR+QLEDEI  RRH D+SI +I KLLFGQA+SSE+L+NVR QGQSLVDDW 
Sbjct: 361 PFGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWG 420

Query: 421 CFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCL 457
           CFK FVK YEKHC R+S YGMKYTRA+ANICNAGIT++QMDQACL+TCL
Sbjct: 421 CFKKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCL 467

BLAST of PI0027772 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 489.2 bits (1258), Expect = 4.1e-138
Identity = 235/429 (54.78%), Postives = 307/429 (71.56%), Query Frame = 0

Query: 39  TGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGI 98
           +G RWA+L+AGS+GY NYRHQAD+CHAYQ+L+KGG+ +ENI+VFMYDDIA +  NPRPG 
Sbjct: 56  SGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGT 115

Query: 99  IINKPDG------------DDHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYS 158
           IIN P G             D VN  N +A ILG+K+A+ GGSGKV+ SGP+DHIFI+YS
Sbjct: 116 IINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYS 175

Query: 159 DHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENIKI 218
           DHG  G+LGMP   Y++ NDL DVLK+KH   +YKS+V Y+EACESGS+FEG+LPE + I
Sbjct: 176 DHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNI 235

Query: 219 YATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYH 278
           YATTASNA E SW TYCP E   PPP +Y TCLGDL+SVAWMEDS  H+L  ETL+QQY 
Sbjct: 236 YATTASNAEESSWGTYCPGEEPSPPP-EYETCLGDLYSVAWMEDSGMHNLQTETLHQQYE 295

Query: 279 TVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLS 338
            V+RRT      YGSHVM YG+  I  D L+ ++G +P  DN+  +     ++  PS+++
Sbjct: 296 LVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNF--TFADANSLKPPSRVT 355

Query: 339 SNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFGQA 398
               +QRDA L+H+  K+++AP GS  K EA++Q+ + +  R H DNS+  +GK+LFG +
Sbjct: 356 ----NQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGIS 415

Query: 399 RSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQ 456
           R  E+L+ VR+ GQ LVDDW C K  V+ +E+HC  +S YG+K+ R+ ANICNAGI M Q
Sbjct: 416 RGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQ 475

BLAST of PI0027772 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 481.1 bits (1237), Expect = 1.1e-135
Identity = 232/431 (53.83%), Postives = 310/431 (71.93%), Query Frame = 0

Query: 37  DETGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHPSNPRP 96
           D+   +WA+L+AGS+GY NYRHQADVCHAYQ+LKKGG+ +ENI+VFMYDDIA +  NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 97  GIIINKPDGD------------DHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIY 156
           G+IIN P+G+            D VN  N  A ILGNK+AL GGSGKV+ SGP+DHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 157 YSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGVLPENI 216
           YSDHG  G+LGMP    ++ NDL DVLK+K+ + +YKS+V Y+EACESGS+FEG+LPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 217 KIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQ 276
            IYATTASNA E SW TYCP E    PP +Y TCLGDL+SVAW+EDS+KH+L  ETL++Q
Sbjct: 219 NIYATTASNAEESSWGTYCPGED-PSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQ 278

Query: 277 YHTVRRRTFADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQTISLPSK 336
           Y  V++RT      YGSHVM +G+  +  + L  F+G +P  +N+  +  +  +I  PS+
Sbjct: 279 YELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENF--TFVNENSIRPPSR 338

Query: 337 LSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINNIGKLLFG 396
           ++    +QRDA L+H+ +K+Q+AP GS  K+EA++Q+ + +  R H DNSI  IG LLFG
Sbjct: 339 VT----NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG 398

Query: 397 QARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITM 456
                 +L+ VR  G+ LVDDW C K+ V+ +E+HC  +S YG+K+ R+IAN+CNAGI M
Sbjct: 399 -LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQM 458

BLAST of PI0027772 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 466.5 bits (1199), Expect = 2.8e-131
Identity = 232/463 (50.11%), Postives = 308/463 (66.52%), Query Frame = 0

Query: 10  KLLIILITLLCFNNIIAKNEWISDATTDET-----GKRWALLIAGSNGYDNYRHQADVCH 69
           ++L+ L  LL F+    K + ++D   + +     G RWA+L+AGSN Y NYRHQAD+CH
Sbjct: 9   QILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICH 68

Query: 70  AYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------HVNAV 129
           AYQIL+KGG+ DENIIVFMYDDIAF   NPRPG+IINKPDG+D             VN  
Sbjct: 69  AYQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQ 128

Query: 130 NFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLIDVLK 189
           NFY  +LGN+S +TGG+GKV+ SGP+D+IFIYY+DHGA G++ MP GD +   D  +VL+
Sbjct: 129 NFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLE 188

Query: 190 RKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESHRPPP 249
           + HK K Y  MVIYVEACESGSMFEG+L +N+ IYA TA+N+ E SW  YCP ES+ PPP
Sbjct: 189 KMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCP-ESYPPPP 248

Query: 250 KDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGNKRIG 309
            +  TCLGD FS++W+EDSD HD+SKETL QQYH V+RR  +D     SHV  +G +++ 
Sbjct: 249 SEIGTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVP-ETSHVCRFGTEKML 308

Query: 310 NDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAPFGSR 369
            D+L++++G +P+ DN+  +   +  IS     +S  V+ RD  L++ Q K Q+AP GS 
Sbjct: 309 KDYLSSYIGRNPENDNFTFTESFSSPIS-----NSGLVNPRDIPLLYLQRKIQKAPMGSL 368

Query: 370 EKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQCFKTF 429
           E  EA+++L DE   R+  D SI +I +L   Q     +L + R  GQ LVDDW CFKT 
Sbjct: 369 ESKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTL 428

Query: 430 VKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTC 456
           V  ++ HC     YG+KYT A+ANICN G+ + Q   A  Q C
Sbjct: 429 VNSFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQAC 464

BLAST of PI0027772 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 446.8 bits (1148), Expect = 2.3e-125
Identity = 242/517 (46.81%), Postives = 325/517 (62.86%), Query Frame = 0

Query: 10  KLLIILITLLCFNNIIAKNEWISDATTDET-----GKRWALLIAGSNGYDNYRHQADVCH 69
           ++L+ L  LL F+    K + ++D   + +     G RWA+L+AGSN Y NYRHQAD+CH
Sbjct: 9   QILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICH 68

Query: 70  AYQILKKGGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGDD------------HVNAV 129
           AYQIL+KGG+ DENIIVFMYDDIAF   NPRPG+IINKPDG+D             VN  
Sbjct: 69  AYQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQ 128

Query: 130 NFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPDGDYIFVNDLIDVLK 189
           NFY  +LGN+S +TGG+GKV+ SGP+D+IFIYY+DHGA G++ MP GD +   D  +VL+
Sbjct: 129 NFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLE 188

Query: 190 RKHKAKSYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKESHRPPP 249
           + HK K Y  MVIYVEACESGSMFEG+L +N+ IYA TA+N+ E SW  YCP ES+ PPP
Sbjct: 189 KMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCP-ESYPPPP 248

Query: 250 KDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHTVRRRTFADKSGYGSHVMLYGNKRIG 309
            +  TCLGD FS++W+EDSD HD+SKETL QQYH V+RR  +D     SHV  +G +++ 
Sbjct: 249 SEIGTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVP-ETSHVCRFGTEKML 308

Query: 310 NDFLNTFLGNSPDYDNYKSSVQSTQTISLPSKLSSNAVSQRDASLIHYQYKFQEAPFGSR 369
            D+L++++G +P+ DN+  +   +  IS     +S  V+ RD  L++ Q K Q+AP GS 
Sbjct: 309 KDYLSSYIGRNPENDNFTFTESFSSPIS-----NSGLVNPRDIPLLYLQRKIQKAPMGSL 368

Query: 370 EKMEAREQLEDEIFKRRHADNSINNIGKLLFGQARSSEILHNVRAQGQSLVDDWQCFKTF 429
           E  EA+++L DE   R+  D SI +I +L   Q     +L + R  GQ LVDDW CFKT 
Sbjct: 369 ESKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTL 428

Query: 430 VKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTC-LERRRRREARRRKKSI 489
           V  ++ HC     YG+KYT A+ANICN G+ + Q   A  Q    E RR R      K  
Sbjct: 429 VNSFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQASNQENRRNRRYETNIKDR 488

Query: 490 KMEEEEDDNARKRKNEKKKERRKKTTAKWRRNKEEVV 509
            +  E  D +R  +++    R        R  ++  V
Sbjct: 489 LVSGELSDGSRPARDQNGVPRGHLAVYVGREERQRFV 518

BLAST of PI0027772 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 444.5 bits (1142), Expect = 1.1e-124
Identity = 221/438 (50.46%), Postives = 295/438 (67.35%), Query Frame = 0

Query: 32  SDATTDETGKRWALLIAGSNGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIAFHP 91
           +D   D  G RWA+L+AGS+GY NYRHQADVCHAYQIL+KGG+ +ENI+V MYDDIA HP
Sbjct: 41  ADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHP 100

Query: 92  SNPRPGIIINKPDGDD------------HVNAVNFYAAILGNKSALTGGSGKVIHSGPHD 151
            NPRPG +IN PDGDD             V A NFYA +LG++ A+ GGSGKVI S P+D
Sbjct: 101 LNPRPGTLINHPDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPND 160

Query: 152 HIFIYYSDHGAAGMLGMPDGDYIFVNDLIDVLKRKHKAKSYKSMVIYVEACESGSMFEGV 211
           HIF+YY+DHG  G+LGMP+  +I+  D I+ LK+KH + +YK MVIYVEACESGS+FEG+
Sbjct: 161 HIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGI 220

Query: 212 LPENIKIYATTASNATEDSWATYCPKESHRPPPKDYNTCLGDLFSVAWMEDSDKHDLSKE 271
           +P+++ IY TTASNA E S+ TYCP   +  PP +Y TCLGDL+SVAWMEDS+ H+L KE
Sbjct: 221 MPKDLNIYVTTASNAQESSYGTYCP-GMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKE 280

Query: 272 TLNQQYHTVRRRT--FADKSGYGSHVMLYGNKRIGNDFLNTFLGNSPDYDNYKSSVQSTQ 331
           T+ QQYHTV+ RT  +   SG GSHVM YGN  I ++ L  + G  P   N   +     
Sbjct: 281 TIKQQYHTVKMRTSNYNTYSG-GSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLN----- 340

Query: 332 TISLPSKLSSNAVSQRDASLIHYQYKFQEAPFGSREKMEAREQLEDEIFKRRHADNSINN 391
              LP K     V+QRDA L+   + ++ +  GSR+K +  ++L +    R+H D S+  
Sbjct: 341 --ELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVEL 400

Query: 392 IGKLLFGQARSSEILHNVRAQGQSLVDDWQCFKTFVKIYEKHCNRMSSYGMKYTRAIANI 451
           I  +LFG   +  +L+ VR  G  LVDDW+C K+ V+++E+HC  ++ YGMK+ RA AN+
Sbjct: 401 IATILFGP--TMNVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANV 460

Query: 452 CNAGITMHQMDQACLQTC 456
           CN G++   M++A    C
Sbjct: 461 CNNGVSKELMEEASTAAC 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P490444.7e-13956.84Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1[more]
O243252.3e-13856.41Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
P490431.1e-13755.92Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1[more]
Q391195.7e-13754.78Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
P490471.6e-13453.83Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g... [more]
Match NameE-valueIdentityDescription
A0A5A7TXA71.9e-24490.60Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DZP19.5e-23688.46Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3CJ433.9e-20592.49vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1D4755.8e-20173.56vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia ... [more]
A0A6J1JF371.3e-20075.64vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
KAA0046547.13.9e-24490.60vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa][more]
XP_038880044.13.8e-23986.54vacuolar-processing enzyme-like [Benincasa hispida][more]
TYK28710.12.0e-23588.46vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa][more]
XP_008463400.28.0e-20592.49PREDICTED: vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo][more]
XP_022148051.11.2e-20073.56vacuolar-processing enzyme gamma-isozyme-like isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G32940.14.1e-13854.78gamma vacuolar processing enzyme [more]
AT2G25940.11.1e-13553.83alpha-vacuolar processing enzyme [more]
AT3G20210.12.8e-13150.11delta vacuolar processing enzyme [more]
AT3G20210.22.3e-12546.81delta vacuolar processing enzyme [more]
AT1G62710.11.1e-12450.46beta vacuolar processing enzyme [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 471..493
NoneNo IPR availableGENE3D1.10.132.130coord: 332..465
e-value: 2.5E-34
score: 120.1
NoneNo IPR availableGENE3D3.40.50.1460coord: 40..324
e-value: 1.0E-111
score: 374.9
NoneNo IPR availablePIRSRPIRSR019663-1PIRSR019663-1coord: 40..457
e-value: 6.3E-181
score: 600.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 463..510
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 468..485
NoneNo IPR availablePANTHERPTHR12000:SF45VACUOLAR-PROCESSING ENZYME-LIKEcoord: 28..456
IPR001096Peptidase C13, legumainPRINTSPR00776HEMOGLOBNASEcoord: 68..97
score: 61.45
coord: 180..196
score: 67.26
coord: 43..67
score: 72.0
IPR001096Peptidase C13, legumainPIRSFPIRSF019663Legumaincoord: 5..470
e-value: 1.8E-190
score: 631.8
IPR001096Peptidase C13, legumainPFAMPF01650Peptidase_C13coord: 42..301
e-value: 1.6E-97
score: 326.0
IPR001096Peptidase C13, legumainPANTHERPTHR12000HEMOGLOBINASE FAMILY MEMBERcoord: 28..456
IPR043577Asparaginyl endopeptidasePIRSFPIRSF500139AEcoord: 8..471
e-value: 2.8E-190
score: 631.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027772.1PI0027772.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006624 vacuolar protein processing
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008233 peptidase activity