PI0027475 (gene) Melon (PI 482460) v1

Overview


Name	PI0027475
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	RNA pseudouridine synthase 1
Location	chr05: 3952134 .. 3954884 (-)
RNA-Seq Expression	PI0027475
Synteny	PI0027475

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACACTTTAAAAAAACTATGTTATTTGCTATTTTCCAAACTCTTACTAATTACAAAAATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCACCAAATTCCCATTCACAAAGCCCTTCTGTTTCCATAGATTCAAATCCGTCGGTAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTGCCCCTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCCGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTCGGTGATTGGGCTAAGGATGGTTTGTGAATCTTACTGTGAAAGCGTTTTTTTTTTCAGACGAATTCGAATTTTAACATGAACTTGGCAAGACGAATTCAATTTTTCCCTACATTTTGGATTTGCTAATCGAATGGTAATGGTGGATTTGCAACTTTTAACTTGCTTTCGTTTGAAAATTTGTAAAAAGGGTATGTATAATCATGTGCTTTCCATGGATTACGAAAGATGTTAGGACTGAGTTTTCGTTGAAATTGACGGGTTTTGAAAGATTTTTTCCTTATAGATCATTTGCATTGATTTTCTAGATCAAACTTTTGTATATTGATAATGTAACTTGGTTTTTGTTGAAGTTAGCTGAAAGTAATGGGTGAATGTGATGGAAGTTTAACAAAAGTTTAAAATTGTAATGCTTATCTATGTGTAAGGTCCAATTTGATGAAAATTTGAAGTTTGGAGTAATAATTGATTCATATTGCCAAGTTTTTGGGTAGGATTTGATCTTTCATCAAATTTGTTTAGAACAAGTGATTTTCTATTGCTTTAAGCTGTTTAAAAATGCAATTTCTCAAGTTCTATTTCCTCGTCTGTAACTAGCTTTTTGTAATTATTATATAGGTGTTATTTTGCGTAGTTGGAGTCCCTCCATACAGGGAGAGGTGCCTTTTCTGTGGGCTGTGCTTTTTTTATGCCGGTGTATTTTTTCTTTTTTTTTCAATAAAAGCCGGCGTTTCTAAAAGAAAACAATACATTTACTTAAGAAGCAAGGGCGAGTAACTGATTTCTGATTCTTTCCCTACATTGCCTAGCAATGTTGCTGATTGGTAGATAGTTGATATAAATGGTTTAGATGTATCCTGATTGTTGAAGAGAGAAGTGCTTGAATGTTTCTGAAGTGTTTTAACGTTTGTCTAAAACAATGGTGTGTAATTACATCTTTCGCTAATTCTATTGTTATCGATTTCTCGGGAGTGTTCTTAGCTCGAATTCTTCATATGCAGGCATCAAAACCAGTTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACGGTTTTAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGGAAGATGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGAACATTGATAGTCAGAAGGACGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTCGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGAAAAATTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCTAGCATTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTAATTATACAAAGAGATTTGGTGTCGAAGAATGTTGCTGAAGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGATCAATCAAATTTGTAAAGTGCAACTGCAGACAGCTTTCGTTCTGTCAGGTTGCAGCTGTCTGCAGTTTTTCATCTATTGAATGAGCTCTTGTTTGCACAACAGTGTGTACAAAAATATCAGCAGAGTCGTAAACCAATCAACCATCCGAGTTCTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACAGAAGAATCATGTGTGTCGAAGGCTGATAACTTTGTGCAAAGCCCTTTGGAAATGGATCTTCTGCTGCTGTTTTATCGATAAAATTGATGTACAGTGTCGAAACAGCCAAACTCAATGGTTAGCACGTTGTTGATTTATGTTGAGGCTCGATTGTTATTTGACTCCCCTTCCATCCATTTGTGCAACTTTGCGATCAAAATATGATGAACAATTTGTTATGATGTTTATGCGGCTATGCATTATAAATAGAAGAATGAGAAAACATGCTGAGGTTTATATA

mRNA sequence

GACACTTTAAAAAAACTATGTTATTTGCTATTTTCCAAACTCTTACTAATTACAAAAATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCACCAAATTCCCATTCACAAAGCCCTTCTGTTTCCATAGATTCAAATCCGTCGGTAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTGCCCCTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCCGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTCGGTGATTGGGCTAAGGATGGCATCAAAACCAGTTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACGGTTTTAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGGAAGATGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGAACATTGATAGTCAGAAGGACGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTCGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGAAAAATTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCTAGCATTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTAATTATACAAAGAGATTTGGTGTCGAAGAATGTTGCTGAAGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGATCAATCAAATTTGTAAAGTGCAACTGCAGACAGCTTTCGTTCTGTCAGGTTGCAGCTGTCTGCAGTTTTTCATCTATTGAATGAGCTCTTGTTTGCACAACAGTGTGTACAAAAATATCAGCAGAGTCGTAAACCAATCAACCATCCGAGTTCTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACAGAAGAATCATGTGTGTCGAAGGCTGATAACTTTGTGCAAAGCCCTTTGGAAATGGATCTTCTGCTGCTGTTTTATCGATAAAATTGATGTACAGTGTCGAAACAGCCAAACTCAATGGTTAGCACGTTGTTGATTTATGTTGAGGCTCGATTGTTATTTGACTCCCCTTCCATCCATTTGTGCAACTTTGCGATCAAAATATGATGAACAATTTGTTATGATGTTTATGCGGCTATGCATTATAAATAGAAGAATGAGAAAACATGCTGAGGTTTATATA

Coding sequence (CDS)

ATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCACCAAATTCCCATTCACAAAGCCCTTCTGTTTCCATAGATTCAAATCCGTCGGTAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTGCCCCTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCCGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTCGGTGATTGGGCTAAGGATGGCATCAAAACCAGTTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACGGTTTTAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGGAAGATGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGAACATTGATAGTCAGAAGGACGAGATCTTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTCGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGAAAAATTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCTAGCATTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAA

Protein sequence

MALFISLHPNFSIFPKTFTKFPFTKPFCFHRFKSVGNSMTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASLALQPQQQEVNSPQSFKTMP

Homology

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BLAST of PI0027475 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-99
Identity = 186/302 (61.59%), Postives = 225/302 (74.50%), Query Frame = 0


Query: 52  STGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIY 111
           +TGE YPVP+SPP PA SK++EL RAM AS++S+  A S+  V++EDEWL  V+KP G+Y
Sbjct: 47  ATGE-YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVY 106

Query: 112 CENVLAAVP---RLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 171
           C+ +L+A+P     LGD      + + P LHLANRLDRDTSG+MVITK +KVA KLVKAF
Sbjct: 107 CDALLSALPCSAATLGD------EATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAF 166

Query: 172 TDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 231
           T+H V K+Y+A C+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGSVVRDM T
Sbjct: 167 TEHKVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMST 226

Query: 232 YFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDE----ILIRARPRSGRTH 291
            FEVL +NGK    E   F  DE E+I V  K+  ++ S  DE    IL+RA P+SGRTH
Sbjct: 227 RFEVLGINGKGQFREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTH 286

Query: 292 QIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSW 347
           QIRLHCQYLG PIRGDVKY GV EWNG  YD H LHAESL FVHPVTG+P+  ++PLPSW
Sbjct: 287 QIRLHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSW 338

[Close]

BLAST of PI0027475 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 6.4e-98
Identity = 182/294 (61.90%), Postives = 221/294 (75.17%), Query Frame = 0


Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLK 175
           L + P+++ D        S  E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLD--------SSSEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 176 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 235
           SYIA C+G+SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 236 NG-KNTMEELQKFREDEEETIVVHTKSLVNIDSQKDE--ILIRARPRSGRTHQIRLHCQY 295
           N  KN   EL    ED    IV   +  ++     D+  +++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKNESCEL----EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 296 LGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS 347
           LGIPIRGDVKY GV EWNG+ ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of PI0027475 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 6.5e-18
Identity = 77/251 (30.68%), Postives = 119/251 (47.41%), Query Frame = 0


Query: 94  VIYEDEWLIAVNKPQGIYCENVLAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMV 153
           +IYED+ LIA+NKP  +  +    +   L  D A   +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 154 ITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 213
           I K++  + KL  AF +  V+K Y A   G   K   I VKS  G+SK    R++   D+
Sbjct: 151 IAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSNIGKSK---GRMFKITDI 210

Query: 214 GRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDEIL 273
                               S NGK                 + + K L ++++  +  L
Sbjct: 211 D-------------------SDNGK---------------LAITYYKLLKSLNN--NLFL 270

Query: 274 IRARPRSGRTHQIRLHCQYLGIPIRGDVKY--EGVTEWNGKIYDSHELHAESLYFVHPVT 333
           I   P +GR HQ+RLH Q LG PI GD KY  + V  ++  ++    LHA ++Y    + 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKYMF----LHANNIYLSESIV 295

Query: 334 GIPLKLQAPLP 343
           G  +KL+A LP
Sbjct: 331 GKEIKLEAKLP 295

[Close]

BLAST of PI0027475 vs. ExPASy Swiss-Prot
Match: Q87S65 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=rluD PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.9e-17
Identity = 82/291 (28.18%), Postives = 123/291 (42.27%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQ--------GIYCENVLAAVPRLLGDWAKDGIKTSLPELHLANRL 152
           D++YED+ +I +NKP+        G     VL A+     D A+      +P   + +RL
Sbjct: 85  DIVYEDDDIIVINKPRDFVVHPGAGTPDGTVLNALLHHYPDIAE------VPRAGIVHRL 144

Query: 153 DRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGV 212
           D+DT+G+MV+ K+    ++LV+A     + + Y A  +G      K+    G   +K   
Sbjct: 145 DKDTTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDQPIGRHSTK--- 204

Query: 213 WRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVN 272
             + A A +G         +   T++ V            + FRE        HT+    
Sbjct: 205 RTLMAVAPLG---------KPAVTHYRV-----------AEHFRE--------HTR---- 264

Query: 273 IDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG--------VTEWNGKI--Y 332
                    IR R  +GRTHQIR+H  YL  P+ GD  Y G          E    I  +
Sbjct: 265 ---------IRLRLETGRTHQIRVHMSYLQHPLLGDTAYGGRARIPTGASQELTDMIRGF 324

Query: 333 DSHELHAESLYFVHPVTGIPLKLQAPLPS---WASLALQPQQQEVNSPQSF 363
           D   LHA  L F HP+TG  L+  AP+P      + AL+   +E   P  F
Sbjct: 325 DRQALHAVMLRFEHPITGEELEFHAPVPDDMVAMTEALRKDTEEYGLPDEF 325

[Close]

BLAST of PI0027475 vs. ExPASy Swiss-Prot
Match: Q9ZDR7 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-16
Identity = 70/251 (27.89%), Postives = 115/251 (45.82%), Query Frame = 0


Query: 94  VIYEDEWLIAVNKPQGIYCENVLAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMV 153
           +IYED+ LIA+NKP  +  +    +   L  D A   +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 154 ITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 213
           I K++  + KL  AF +  V+K Y A   G  P      VKS  G+S             
Sbjct: 151 IAKNYLSSVKLHNAFKEKLVIKKYFAITYG-RPVKNVGTVKSNIGKS------------- 210

Query: 214 GRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDEIL 273
                                   K  + ++     D+ +  + + K L ++++  +  L
Sbjct: 211 ------------------------KRRLSKIANIDSDDGKLAITYYKLLKSLNN--NLFL 270

Query: 274 IRARPRSGRTHQIRLHCQYLGIPIRGDVKY--EGVTEWNGKIYDSHELHAESLYFVHPVT 333
           I   P +GR HQ+RLH Q LG PI GD KY  + +  ++  ++    LHA ++Y    + 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEIMPYSKYMF----LHANNIYLSETIV 295

Query: 334 GIPLKLQAPLP 343
           G  +KL+A LP
Sbjct: 331 GKEIKLEAKLP 295

[Close]

BLAST of PI0027475 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 6.2e-197
Identity = 347/366 (94.81%), Postives = 351/366 (95.90%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFTKFPFTKPFCFHRFKSVGNSMTDSSEPVTGNCPSTGENYPVP 60
           MALFISLHPNFSI PKTFT+FPFTK FCFHRFKSV NSMTDSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAF 180
           RLLGD A  GIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTV KSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240
           CVGTSPKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEELQKFR  EEETIVVHTKSLV+IDS KDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASLALQPQQQEVNSPQ 360
           VKYEGVTEWN KIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS ALQPQQ EVNSPQ
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQ 360

Query: 361 SFKTMP 367
           SFKTMP
Sbjct: 361 SFKTMP 366

[Close]

BLAST of PI0027475 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.0e-178
Identity = 314/328 (95.73%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCENVLAAVPRLL D A  GIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTV KSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARP 278
           GGS+VRDMETYFEVLSVNGKNTMEELQKFR++EEETIVVHTKSLV+IDSQKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASLALQPQQQEVNSPQSFKTMP 367
           APLPSWAS ALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of PI0027475 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.0e-178
Identity = 314/328 (95.73%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCENVLAAVPRLL D A  GIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTV KSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARP 278
           GGS+VRDMETYFEVLSVNGKNTMEELQKFR++EEETIVVHTKSLV+IDSQKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASLALQPQQQEVNSPQSFKTMP 367
           APLPSWAS ALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of PI0027475 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.1e-156
Identity = 280/330 (84.85%), Postives = 303/330 (91.82%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDWA-----KDGIKTSLPELHLANRLDRDTSGVMV 158
           EWLIAVNKPQG+YCE+VLA+VPRLLGD A       GI+ SLPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 159 ITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 218
           ITKSHKVASKLVKAFTDH V KSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 219 GRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDEIL 278
           GRSLPGGSVVRDMETYFEVLSVNG++TM+EL + R+DEEETIVV +KSL++ID+ KDEIL
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 279 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGI 338
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG IYDSHELHAESL+FVHPVTGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 339 PLKLQAPLPSWASLALQPQQQEVNSPQSFK 364
           PLKLQAPLPSWAS AL  QQQEV+SP + K
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLK 330

[Close]

BLAST of PI0027475 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.6e-155
Identity = 280/340 (82.35%), Postives = 303/340 (89.12%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDWAKDG---------------IKTSLPELHLANR 158
           EWLIAVNKPQG+YCE+VLA+VPRLLGD A  G               I+ SLPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 159 LDRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFG 218
           LDRDTSGVMVITKSHKVASKLVKAFTDH V KSYIAFCVG++PKWKKI+VKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 219 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLV 278
           VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNG++TM+EL + R+DEEETIVV +KSL+
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 279 NIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAES 338
           +ID+ KDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG IYDSHELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 339 LYFVHPVTGIPLKLQAPLPSWASLALQPQQQEVNSPQSFK 364
           L+FVHPVTGIPLKLQAPLPSWAS AL  QQQEV+SP + K
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLK 340

[Close]

BLAST of PI0027475 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 1.3e-196
Identity = 347/366 (94.81%), Postives = 351/366 (95.90%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFTKFPFTKPFCFHRFKSVGNSMTDSSEPVTGNCPSTGENYPVP 60
           MALFISLHPNFSI PKTFT+FPFTK FCFHRFKSV NSMTDSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAF 180
           RLLGD A  GIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTV KSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240
           CVGTSPKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEELQKFR  EEETIVVHTKSLV+IDS KDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASLALQPQQQEVNSPQ 360
           VKYEGVTEWN KIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS ALQPQQ EVNSPQ
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQ 360

Query: 361 SFKTMP 367
           SFKTMP
Sbjct: 361 SFKTMP 366

[Close]

BLAST of PI0027475 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa] >TYJ96474.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 636.0 bits (1639), Expect = 2.1e-178
Identity = 314/328 (95.73%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCENVLAAVPRLL D A  GIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTV KSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARP 278
           GGS+VRDMETYFEVLSVNGKNTMEELQKFR++EEETIVVHTKSLV+IDSQKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASLALQPQQQEVNSPQSFKTMP 367
           APLPSWAS ALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of PI0027475 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 631.7 bits (1628), Expect = 3.9e-177
Identity = 318/359 (88.58%), Postives = 331/359 (92.20%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFTKFPFTKPFCFHRFKSVGNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL ISL P FS   KTFT+FP  KPFC  RFKS+  SM DSSEPVTGNCPST ENYPVP
Sbjct: 1   MALLISLQPTFSTPHKTFTEFP--KPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120

Query: 121 RLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAF 180
           RLL D A  GIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTV KSYIAF
Sbjct: 121 RLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240
           CVG++PKWKKI++KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLS+NG+N 
Sbjct: 181 CVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNA 240

Query: 241 MEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEE QKF +D EET+VV TKSLV+ID QKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEEFQKFGKD-EETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASLALQPQQQEVNSP 360
           VKYEGVTEWNGKIYD HELHAESLYFVHP+TGIPLKLQAPLPSWAS A QPQQQEVNSP
Sbjct: 301 VKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 356

[Close]

BLAST of PI0027475 vs. NCBI nr
Match: KAG7021056.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 581.6 bits (1498), Expect = 4.6e-162
Identity = 304/375 (81.07%), Postives = 325/375 (86.67%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFTKFPFTKPFCFHRFKSVGNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL +++ P FSI  K+F KFP  KPF FHRF+ V NSM DSS+PV      TGENYPVP
Sbjct: 6   MALVVTIQPPFSIPHKSFAKFP--KPFRFHRFRPVTNSMADSSKPV------TGENYPVP 65

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DVIYEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 66  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQGVYCESVLASVP 125

Query: 121 RLLGDWAKDG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLK 180
           R LGD A  G     IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH V K
Sbjct: 126 RFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 185

Query: 181 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240
           SYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSV
Sbjct: 186 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 245

Query: 241 NGKNTMEELQKFR----EDEEETIVVHTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQ 300
           NG+NT EEL + R    E+EEETIV  +KSL++ID++KDEILIRARPRSGRTHQIRLH Q
Sbjct: 246 NGQNTKEELPESRKDEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQ 305

Query: 301 YLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASLALQP 360
           YLGIPI GDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWA+ ALQP
Sbjct: 306 YLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQP 365

Query: 361 QQQEVNSPQSFKTMP 367
           QQQEVNS  S K MP
Sbjct: 366 QQQEVNSAASLKPMP 372

[Close]

BLAST of PI0027475 vs. NCBI nr
Match: KAG6586224.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 575.1 bits (1481), Expect = 4.3e-160
Identity = 302/377 (80.11%), Postives = 323/377 (85.68%), Query Frame = 0


Query: 1   MALFISLHPNFSIFPKTFTKFPFTKPFCFHRFKSVGNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL ++L   FSI  K+F KFP  KPF FHRF+ V NSM DSS+PV      TGENYPVP
Sbjct: 1   MALVVTLQAPFSIPNKSFAKFP--KPFRFHRFRPVTNSMADSSKPV------TGENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DV+YEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVVYEDEWLIAVNKPQGVYCESVLASVP 120

Query: 121 RLLGDWAKDG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLK 180
             LGD A  G     IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH V K
Sbjct: 121 CFLGDSAMAGEQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180

Query: 181 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240
           SYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSV
Sbjct: 181 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 240

Query: 241 NGKNTMEELQKFR------EDEEETIVVHTKSLVNIDSQKDEILIRARPRSGRTHQIRLH 300
           NG+NT EEL + R      E+EEETIV  +KSL++ID++KDEILIRARPRSGRTHQIRLH
Sbjct: 241 NGQNTKEELPESRKDEEEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLH 300

Query: 301 CQYLGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASLAL 360
            QYLGIPI GDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWA+ AL
Sbjct: 301 SQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQAL 360

Query: 361 QPQQQEVNSPQSFKTMP 367
           QPQQQEVNS  S K MP
Sbjct: 361 QPQQQEVNSAASLKPMP 369

[Close]

BLAST of PI0027475 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 359.0 bits (920), Expect = 4.5e-99
Identity = 182/294 (61.90%), Postives = 221/294 (75.17%), Query Frame = 0


Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLGDWAKDGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLK 175
           L + P+++ D        S  E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLD--------SSSEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 176 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 235
           SYIA C+G+SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 236 NG-KNTMEELQKFREDEEETIVVHTKSLVNIDSQKDE--ILIRARPRSGRTHQIRLHCQY 295
           N  KN   EL    ED    IV   +  ++     D+  +++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKNESCEL----EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 296 LGIPIRGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS 347
           LGIPIRGDVKY GV EWNG+ ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of PI0027475 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 74/304 (24.34%), Postives = 114/304 (37.50%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKP-----------------QGIYCENVLAAVPRLLGD---------W 152
           D++YED+ ++ VNKP                  GI     L  V     +         +
Sbjct: 149 DIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETF 208

Query: 153 AKDGIKTSLPELHLA-------NRLDRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIA 212
           + D   T+ P  + A       +RLD+ T+G++V+ K     + L + F  HT+ + Y++
Sbjct: 209 SDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVS 268

Query: 213 FCVGT-SPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGK 272
              G  SP   +I +  G   S     R+  AA     +PGG              V G 
Sbjct: 269 LTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-----IPGG--------------VRGG 328

Query: 273 NTMEELQKFREDEEETIVVHTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIR 332
                  +++             ++   +     L+  R  +GRTHQIR H +Y+G+P+ 
Sbjct: 329 RARHAASRYK-------------VIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLL 388

Query: 333 GDVKYEGVTEWNGKIY-------DSHE------------LHAESLYFVHPVTGIPLKLQA 344
           GD  Y G       +        D  E            LHA  L F HP TG  +K   
Sbjct: 389 GDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSC 416

[Close]

BLAST of PI0027475 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-09
Identity = 77/279 (27.60%), Postives = 110/279 (39.43%), Query Frame = 0


Query: 52  STGENYPVPLS-----PPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNK 111
           + G+   +PLS     P  P   K      +   S +   F  S   V+Y+D  +I +NK
Sbjct: 145 NVGDRIYLPLSVDNDTPQTPPAKK-----ESFQCSDEERKFVCSL--VLYKDPAIIVLNK 204

Query: 112 PQGIYCENVLAAVPRLLGDWAKDGIKTSLPEL-------------HLANRLDRDTSGVMV 171
           P G+  +                GIKTS+ EL              L +RLDRD SG++V
Sbjct: 205 PHGLAVQ-------------GGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLV 264

Query: 172 ITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 231
           + ++   A+ L   F + T          G S    K +VKS        + R Y A  +
Sbjct: 265 LARTQTAATVLHSIFREKT---------TGASAYGVKKNVKS--------LKRKYMALVI 324

Query: 232 G--------RSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFREDEEETIVVHTKSLVNI 291
           G         S P   VV D +   E ++VN    +   Q             T+  V  
Sbjct: 325 GCPPRQRGQISAPLRKVVVD-DGKSERITVNDNGELVSTQH----------AITEYRVIE 375

Query: 292 DSQKDEILIRARPRSGRTHQIRLHC-QYLGIPIRGDVKY 304
            S      +  RP +GR HQ+R+HC + LG PI GD KY
Sbjct: 385 SSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKY 375

[Close]

BLAST of PI0027475 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.3e-08
Identity = 64/226 (28.32%), Postives = 92/226 (40.71%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQGIYC--------ENVLAAVPRLLGDWAKDGIKTSLPELHLANRL 152
           +V+YED+ LIA+NKP G+            VL  +    G           P     +RL
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRL 157

Query: 153 DRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGV 212
            R TSG+++  K+    +KL   F + T L       VG+      +  + G GR    +
Sbjct: 158 GRGTSGILLCAKTKLAKTKLAAYFAEGTSL-------VGSG----NLDQECGTGRKLSKI 217

Query: 213 WRVYAAA-----DVGRSLPGGSVVR--DMETYFEVLSVNGKNTMEELQKFREDEEETIVV 272
           +R  A       +V    P G VVR   +     V S  GK    ++     D E+    
Sbjct: 218 YRALADGIVEEDEVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNC-- 277

Query: 273 HTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 304
              SLV ++ Q           SGR HQIR+H  Y+G P+ GD  Y
Sbjct: 278 ---SLVKVEIQ-----------SGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]

BLAST of PI0027475 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 53.9 bits (128), Expect = 3.1e-07
Identity = 61/220 (27.73%), Postives = 89/220 (40.45%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQGIYC--------ENVLAAVPRLLGDWAKDGIKTSLPELHLANRL 152
           +V+YED+ LIA+NKP G+            VL  +    G           P     +RL
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRL 157

Query: 153 DRDTSGVMVITKSHKVASKLVKAFTDHTVLKSYIAFCVGTSPKWKKIHVKSGHGRSKFGV 212
            R TSG+++  K+    +KL   F + T L       VG+      +  + G GR    +
Sbjct: 158 GRGTSGILLCAKTKLAKTKLAAYFAEGTSL-------VGSG----NLDQECGTGRKLSKI 217

Query: 213 WRVYAAA-----DVGRSLPGGSVVR--DMETYFEVLSVNGKNTMEELQKFREDEEETIVV 272
           +R  A       +V    P G VVR   +     V S  GK    ++     D E+    
Sbjct: 218 YRALADGIVEEDEVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNC-- 277

Query: 273 HTKSLVNIDSQKDEILIRARPRSGRTHQIRLHCQYLGIPI 298
              SLV ++ Q           SGR HQIR+H  Y+G P+
Sbjct: 278 ---SLVKVEIQ-----------SGRPHQIRIHLAYMGHPL 289

The following BLAST results are available for this feature:

BLAST of PI0027475 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QNM3	2.0e-99	61.59	RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05...	[more]
Q7XA65	6.4e-98	61.90	RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S...	[more]
Q68XB2	6.5e-18	30.68	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC...	[more]
Q87S65	1.9e-17	28.18	Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus sero...	[more]
Q9ZDR7	1.2e-16	27.89	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai...	[more]

BLAST of PI0027475 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJY6	6.2e-197	94.81	PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03...	[more]
A0A5A7TX25	1.0e-178	95.73	RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BW87	1.0e-178	95.73	RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1	[more]
A0A6J1DQ12	1.1e-156	84.85	RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1DT56	2.6e-155	82.35	RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of PI0027475 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144814.3	1.3e-196	94.81	RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ...	[more]
XP_008453651.1	2.1e-178	95.73	PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudou...	[more]
XP_038889468.1	3.9e-177	88.58	RNA pseudouridine synthase 1 [Benincasa hispida]	[more]
KAG7021056.1	4.6e-162	81.07	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6586224.1	4.3e-160	80.11	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of PI0027475 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56345.1	4.5e-99	61.90	Pseudouridine synthase family protein	[more]
AT1G76050.2	1.1e-12	24.34	Pseudouridine synthase family protein	[more]
AT3G19440.1	1.1e-09	27.60	Pseudouridine synthase family protein	[more]
AT3G52260.2	1.3e-08	28.32	Pseudouridine synthase family protein	[more]
AT3G52260.1	3.1e-07	27.73	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 89..342 e-value: 1.0E-44 score: 154.7
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 51..350
None	No IPR available	PANTHER	PTHR21600:SF47	RNA PSEUDOURIDINE SYNTHASE 1	coord: 51..350
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 101..291 e-value: 1.2E-22 score: 80.7
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 101..326 e-value: 2.75415E-39 score: 136.313
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 141..155
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 92..350

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0027475.1	PI0027475.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding