PI0027353 (gene) Melon (PI 482460) v1

Overview
NamePI0027353
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionvacuolar protein sorting-associated protein 45 homolog
Locationchr03: 10058228 .. 10063554 (-)
RNA-Seq ExpressionPI0027353
SyntenyPI0027353
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGCTGATTTCGGTCGTTCGGGATTACATCAACAAGATGTTGCAGGACATCTCCGGCATGAAGGTTCTCATCCTTGATTCTCAGACGGTATGCGCCATCATAGTCATTCCACTCCTTCAGTTTTCTTTCTGATTGGTATCGAGGAGGAAATCAAATGTAGAAGTACGGAATTTTGTTCTGGTCTGGGGGAGGGGATTTGATTTCAATTTTCTGCATACTGTATTGGTCTGAGAAAGTGGGAAAATGTATATTATGGTATCCTTTAGCTGTATTTCAGAGCGTACTCTGATAATTTGCCCCGGGTAGTTGTTGTATATGATTTCTTCGATCTCTTTGAACGTGGAGCTGGATCGTCTATGTATTCCAAGGGGAAGAAATGTTTAATTCTTATATCATTGACAATGCTTAATATCGATTTTAATAATCTAGACTGGAATGAGTTAGTTTTTTAAGGAATATTGATTGCCAGTTCTCCCTGTTTAAGCTTTTAGATAGCAATGTGTTAGGTCCTCTAGTCCAAAATTTTATTTCCTGATTCTTCATGTGTAACGTTTTGCATGGTTGTTGAATAAATGTGTCGTCATAATGTAAAATTGGTGTCATGGTATCTTTTATAATATATTGCAATCATGCTAGAATTGGACCTCCTTTGTGAAATAGCTTGGGGAAAGGCGTGTTTGATGATGTGATATTTGCATCATAACGTTATTATTATTTTTGGTTTCAGGTTAGTGTTGTCAGTGTGGTGTACTCACAGTCGGAGCTTCTTCAAAAAGAAGTCTTTTTGGTTGAATTGGTAGATGCCGTATCGAAGTCAAGTGAATCCATGTATCATCTTAAAGCAGTTTGCTTTCTCAGGCCCACGTCAGAGAATATACAGCTTTTGCGTCGCCAGCTGGCTAATCCTAGATTTGGGGAGTATCACCTTTGTGAGTTCTTCATATCTTTTATGGATTTCTAAGATGTGCTAATAACTTCAATATACGTTAATCATTCTTCTGTATATCTGAATTACAGTTTTCTCCAACATTTTGAAGGAAACTCAAATTCATATACTAGCTGATTCAGATGAGCAGGATGTCGTTCAGCAAGTTGTGGAATTTTCTTCTACAGGAATTATAAGACATAAGTCATGGAGAAATCCACATTAAGTTGTTAAGTAGAATGGTATCTGTCCTGGAATCACGGTCCTTCCCTTGTCAAAATAGATGGCTAGTTTATCAATGGTATGAACCTTATTGTGTGTGTTATTGATCCTGTTCATTAGCCTGAGAGTTGAACTCCATTCTCTGCTCATCCTGTTCATCATCTGATATAGCTGGTCTGCATGAGTGTATATTGTATTGTATTCATGTTTTAGCTTTAATATTGATAATCATACATAATTATTGAATGGATCATGCAGGAGTTTTATGGGGATTTTGTGGCCATTGATCCTTATCATTTCACTTTAAATATGCCATCAAACCACTATACATGATTCCGGCAGTTGTTGATCCTCCTAGTTTGCAACATTTCTGTGACCGAGTTGTAGATGGCATTGCAGCACTTTTCTTGGCCTTAAAACAGAGACCTGTTATTCGGTACCAAAGGACTTCTGATGTTGCCAAGCGGATAGCACAGGAAGCATCAGTACGTAAATTCTTACAATTATAAGTTGCATTTTGTTACTTTAATTTGTCAGTTACCCTTCCTCAATATTACTCTACTAGAAGCGTTCATAACTTCAAATTTGTATTTTATTATCATTTATTATTATTTACACTAGGTTTTATTATCACAAGTTTAAGGGTCATGATCTCTAATTTATATTATTTTTTATGCACCAGAAACTTATGTATCAGCAAGAAAGTGGTCTGTTTGATTTTCGAAGAATGGAAGTTTCCCCTTTGTTGCTGGTAATTGATAGGAGGGATGACCCACTGACTCCCCTGCTGAATCAATGGACTTATCAGGTATTGGAATCACAAAAGATATTTTGCGTTGTTGTTACTATATGTTTGTGTTGGTTATTCATAGTTATGTTTTCTAGGCAATGGTTCATGAATTGCTAGGTATTCAAGACAATAAAGTGGACTTGAAAAGCATTGCAAAATCTTCAAAGGATCAACAGGTATAAAGTTCATTGTTCTTCTCTGCACTAGTATTCATTTTCTATTAGACAATTAAACTCATTCACAATTCTATTCAATTGTGTTTGATCTTTTGGGATCATGCAGGAGGTTGTGCTATCATCAGAGCAAGATTCGTTTTATAAAGCTAACATGTACGAGAATTTTGGAGATATTGGAATGAATATAAAGAGTTGGTGGATGATTTTCAGCAAATTGCCAAAAGTAACCAGAACATACAGACAATAGGTTTGTATGAAATGAAGTTTGAAATTCTTTGTAGGTACTTTTGAAGGATCAACTAGGGGGAGAAACTTGTGTCACTAAATTGTTTTCTTGCTTTGTTTTCTACAGAGGACATGGCCAAGTTTGTTGACAATTATCCAGAGTACAGAAAAATGCACGGTAATGTTTCAAAACATGTGACGTTGGTGACAGAGATGAGTAAAATAGTTGAGGAGCGAAAGCTTATGTTAGTTTCACAGACAGAACAGGAATTGGCTTGCAACGGTGGGCAAGTGGCTGCTTTTGAGGTCATAATATTTTTTTCATCTTTTTTCTCTTTGTGAATCTGCATTGCATTTTTTATTTCATATCTGGATTCTGTACAACATCGACATGTGAAATCATGACATTTTCCTATCTTCAAGTTTTCTTTGACATCCATCTTGGATGTGTTAGCACAAAACTCAATGACTATTATCTGGTTGGTTCTACTATTTTGTTGGGAAATGGGGAGGAGCGATAGGAAAGTTAAAGACCATGTGAGTTATCTTTTTGGGTGTGTGTATGTCAATATGGCAAGAATATGTTACATTGAGTTTGGGGAAGTTATTATGATTGGAACAAATTTTGTAAATGTTAGATTATTGTTGTTTGCAACGTCTCTGCAGCATTTTTTTTCTTATGTGCCCATGAATTATCTGTAAAATGTTTGGACCTTTCAACTTGGCATGGAACTAATGATTAGCTATGAGATTGTTAATTCATGAGATATAGTTGTTACTTTCAGCATGACCAACTCTGTTTTTGTGCATTTCAGGCTGTAACAAATCTTTTGAACAATGAGAGTATTTCTGATATAGACCGTCTGCGGCTTGTTATGTTGTATGCCTTACGGTATGAGAAGGAAAGCCCTGTCCAACTGATGCAGCTTTTCAATAAATTGGCTTCTCGTTCTGCTAAATATAAAACAGGGGTAGTGTATACATTTAAGCGTCATTTTCATTATTTTTCTAATCTTCTACTTCTCTATTTAAAGATTTTTAACATGTTCGATTATTTTATGGGTATTATTTTACTATGCGGTAGCATGATATCCTTCTTTGGGATTTTGAAAAGCTTTCTGTTTCTTCATCCTGTATGTTTGTTAGAAACTACTCATTCTTTGGCAGACTATTATCACAAAATAGTTTACGTACTTCAATTGTAACTAGAGGGGTTAGTCTTCAATTATCTTGATGTGCTCAAAACAACATGATGTGACAGCTTGTCCAGTTTCTTTTAAAACAAGCTGGTGTTGATAAGCGAACGGGTGATCTTTATGGAAATCGAGATCTTTTGAATATTGCTCGTAACATGGCTCGTGGATTAAAGGTACGCTGGAAACTTGTATAAAAGTACACATAATGTCTTCTAGTTTGCAGTTTAGGGTGCTTATTCTCTTCACTTCTGCTAGGGGGTTGAAAACGTATACACTCAGCATCAACCTCTTGTGGTCCAGACCATGGAAAGTATAATCAAGGGACGATTGAGAGATGTGGACTACCCGTTTGTTGGGAATCACTTTCAGCAAGGAAGGTTTGTTTGCTAGTATTAGCATGTCTCAGTCCATATTACTATATATTATATATATTTTTTTTTTGCTAAAACTTTTTCTTTGCATATTGAGTTTTAGGTTAGTTACAAGTTTAGGTTAAACTACAAATTTAATCCCATTAATTTAAAAATGTCTAATTAGGACTTATTAGATACTAATTAAAAATTCAAGAACCTGTTAGACACAAACTTGACAGTTTTTGGATACATTAGATACTTTTTAAAGTTTAGAGACCAAGCTGAAAATTAAGTTCAAGTATCAATGAAAGAGTGAAATTTAGTCTTAATCTTAAGTTATATGGTGTGTGATTGTGTTTGCCATTTTTACATCGATAAATGTTGTTTAGCTTCCTTTGCTAAACTGTGTATGTGTTCAAAGATTGATGGTTCTGATGCTTGTAGCGTATAGTAACTCGTTGTGAAGTGTGATTTTTAATTAGATAAAAATTTGATGAGGCATTCAGATGGCTGCTCTTTAATTCCAACGTCTACGTGCTTAAGTATTTTTCCCAAGAATTAGCTACGCTGTTTCTCAAATCAAATAATCATTTTTTTAAAAAAGAGACAATACCTTTTATCATTGAAATAGAAGTACAAAAAAACGTGGCAGAGGATTAAAAGAAGCCTCACTAGGTCCCGAAAGGTGTTTGTTGTCATGCCTGCCCCTTGAAATATTATTTTGTTACTGTCGTGGCACCCTCTCCATAAAATGGCCATCACTTCAGTGGACCAAAGGGGTCTAGTATTGTCTCGAGAATTAGATGATCATCTGGGTATATTTTTTTTACTAGAAAAAATTCTACTCTCTATTTTCAGAAAGAAACTTCTGTTAAAATATAACTTGGCTACCTTGGTATGAAGCAGGCCACAAGAAGTCATCATTTTCATTGTAGGTGGGACAACATATGAGGAGTCACGTGCTGTAGCTTTACAAAATGCAACTGCTTCTGGAATACGTTTTATCCTAGGTGGTTCTGTGGTTCTAAATTCTAGGCGGTAAGCAATGTTGGTTATTCACTTGTTTGATTTCATGGTCTAGTTTATGAGTTGCTATCACTGCCAATTTCATATTTAATAAAAGGATATTGTCATACTTTGTGTGTCTATATGTGTAGATTTATGTCAATTTATGATATGAATCCTGTTGCTATAGTTGGTAACTGTTTCCATCCAATGAGTTAGACGAGGTGGTTGCAGGAGTAATTAATTAGTGGACGGTCCTACAAGCCACCCTTGCAAATCCCCCTATAATTGAAATCTACTATATAATTGAAATCTCTAAGCAAACATATTGAACAAGTAAATATTTTCATGATATTTGGCTGGCATTCCAGGTTTTTAAAGGACTTGGAAGAAGCTCAGCGGATATCTCGTTCGAGCGCCCCAGTGATTTGA

mRNA sequence

ATGGTGCTGATTTCGGTCGTTCGGGATTACATCAACAAGATGTTGCAGGACATCTCCGGCATGAAGGTTCTCATCCTTGATTCTCAGACGGTTAGTGTTGTCAGTGTGGTGTACTCACAGTCGGAGCTTCTTCAAAAAGAAGTCTTTTTGGTTGAATTGGTAGATGCCGTATCGAAGTCAAGTGAATCCATGTATCATCTTAAAGCAGTTTGCTTTCTCAGGCCCACGTCAGAGAATATACAGCTTTTGCGTCGCCAGCTGGCTAATCCTAGATTTGGGGAGTATCACCTTTTTTTCTCCAACATTTTGAAGGAAACTCAAATTCATATACTAGCTGATTCAGATGAGCAGGATGTCGTTCAGCAAGTTGAGTTTTATGGGGATTTTGTGGCCATTGATCCTTATCATTTCACTTTAAATATTTTGCAACATTTCTGTGACCGAGTTGTAGATGGCATTGCAGCACTTTTCTTGGCCTTAAAACAGAGACCTGTTATTCGGTACCAAAGGACTTCTGATGTTGCCAAGCGGATAGCACAGGAAGCATCAAAACTTATGTATCAGCAAGAAAGTGGTCTGTTTGATTTTCGAAGAATGGAAGTTTCCCCTTTGTTGCTGGTAATTGATAGGAGGGATGACCCACTGACTCCCCTGCTGAATCAATGGACTTATCAGGCAATGGTTCATGAATTGCTAGGTATTCAAGACAATAAAGTGGACTTGAAAAGCATTGCAAAATCTTCAAAGGATCAACAGGAGGTTGTGCTATCATCAGAGCAAGATTCGTTTTATAAAGCTAACATGTACGAGAATTTTGGAGATATTGGAATGAATATAAAGAGTTGGTGGATGATTTTCAGCAAATTGCCAAAAGACATGGCCAAGTTTGTTGACAATTATCCAGAGTACAGAAAAATGCACGGTAATGTTTCAAAACATGTGACGTTGGTGACAGAGATGAGTAAAATAGTTGAGGAGCGAAAGCTTATGTTAGTTTCACAGACAGAACAGGAATTGGCTTGCAACGGTGGGCAAGTGGCTGCTTTTGAGGCTGTAACAAATCTTTTGAACAATGAGAGTATTTCTGATATAGACCGTCTGCGGCTTGTTATGTTGTATGCCTTACGGTATGAGAAGGAAAGCCCTGTCCAACTGATGCAGCTTTTCAATAAATTGGCTTCTCGTTCTGCTAAATATAAAACAGGGCTTGTCCAGTTTCTTTTAAAACAAGCTGGTGTTGATAAGCGAACGGGTGATCTTTATGGAAATCGAGATCTTTTGAATATTGCTCGTAACATGGCTCGTGGATTAAAGGGGGTTGAAAACGTATACACTCAGCATCAACCTCTTGTGGTCCAGACCATGGAAAGTATAATCAAGGGACGATTGAGAGATGTGGACTACCCGTTTGTTGGGAATCACTTTCAGCAAGGAAGGCCACAAGAAGTCATCATTTTCATTGTAGGTGGGACAACATATGAGGAGTCACGTGCTGTAGCTTTACAAAATGCAACTGCTTCTGGAATACGTTTTATCCTAGGTGGTTCTGTGGTTCTAAATTCTAGGCGGTTTTTAAAGGACTTGGAAGAAGCTCAGCGGATATCTCGTTCGAGCGCCCCAGTGATTTGA

Coding sequence (CDS)

ATGGTGCTGATTTCGGTCGTTCGGGATTACATCAACAAGATGTTGCAGGACATCTCCGGCATGAAGGTTCTCATCCTTGATTCTCAGACGGTTAGTGTTGTCAGTGTGGTGTACTCACAGTCGGAGCTTCTTCAAAAAGAAGTCTTTTTGGTTGAATTGGTAGATGCCGTATCGAAGTCAAGTGAATCCATGTATCATCTTAAAGCAGTTTGCTTTCTCAGGCCCACGTCAGAGAATATACAGCTTTTGCGTCGCCAGCTGGCTAATCCTAGATTTGGGGAGTATCACCTTTTTTTCTCCAACATTTTGAAGGAAACTCAAATTCATATACTAGCTGATTCAGATGAGCAGGATGTCGTTCAGCAAGTTGAGTTTTATGGGGATTTTGTGGCCATTGATCCTTATCATTTCACTTTAAATATTTTGCAACATTTCTGTGACCGAGTTGTAGATGGCATTGCAGCACTTTTCTTGGCCTTAAAACAGAGACCTGTTATTCGGTACCAAAGGACTTCTGATGTTGCCAAGCGGATAGCACAGGAAGCATCAAAACTTATGTATCAGCAAGAAAGTGGTCTGTTTGATTTTCGAAGAATGGAAGTTTCCCCTTTGTTGCTGGTAATTGATAGGAGGGATGACCCACTGACTCCCCTGCTGAATCAATGGACTTATCAGGCAATGGTTCATGAATTGCTAGGTATTCAAGACAATAAAGTGGACTTGAAAAGCATTGCAAAATCTTCAAAGGATCAACAGGAGGTTGTGCTATCATCAGAGCAAGATTCGTTTTATAAAGCTAACATGTACGAGAATTTTGGAGATATTGGAATGAATATAAAGAGTTGGTGGATGATTTTCAGCAAATTGCCAAAAGACATGGCCAAGTTTGTTGACAATTATCCAGAGTACAGAAAAATGCACGGTAATGTTTCAAAACATGTGACGTTGGTGACAGAGATGAGTAAAATAGTTGAGGAGCGAAAGCTTATGTTAGTTTCACAGACAGAACAGGAATTGGCTTGCAACGGTGGGCAAGTGGCTGCTTTTGAGGCTGTAACAAATCTTTTGAACAATGAGAGTATTTCTGATATAGACCGTCTGCGGCTTGTTATGTTGTATGCCTTACGGTATGAGAAGGAAAGCCCTGTCCAACTGATGCAGCTTTTCAATAAATTGGCTTCTCGTTCTGCTAAATATAAAACAGGGCTTGTCCAGTTTCTTTTAAAACAAGCTGGTGTTGATAAGCGAACGGGTGATCTTTATGGAAATCGAGATCTTTTGAATATTGCTCGTAACATGGCTCGTGGATTAAAGGGGGTTGAAAACGTATACACTCAGCATCAACCTCTTGTGGTCCAGACCATGGAAAGTATAATCAAGGGACGATTGAGAGATGTGGACTACCCGTTTGTTGGGAATCACTTTCAGCAAGGAAGGCCACAAGAAGTCATCATTTTCATTGTAGGTGGGACAACATATGAGGAGTCACGTGCTGTAGCTTTACAAAATGCAACTGCTTCTGGAATACGTTTTATCCTAGGTGGTTCTGTGGTTCTAAATTCTAGGCGGTTTTTAAAGGACTTGGAAGAAGCTCAGCGGATATCTCGTTCGAGCGCCCCAGTGATTTGA

Protein sequence

MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKSSESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVVQQVEFYGDFVAIDPYHFTLNILQHFCDRVVDGIAALFLALKQRPVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMIFSKLPKDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQTEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLASRSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQTMESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILGGSVVLNSRRFLKDLEEAQRISRSSAPVI
Homology
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BLAST of PI0027353 vs. ExPASy Swiss-Prot
Match: O49048 (Vacuolar protein sorting-associated protein 45 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS45 PE=1 SV=2)

HSP 1 Score: 859.0 bits (2218), Expect = 2.9e-248
Identity = 450/569 (79.09%), Postives = 500/569 (87.87%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVL++ VRDYIN+MLQDISGMKVLILDS+TVS VS+VYSQSELLQKEVFLVE++D++S S
Sbjct: 1   MVLVTSVRDYINRMLQDISGMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDSISVS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            ESM HLKAV F+RPTS+NIQ LR QLANPRFGEYHLFFSN+LK+TQIHILADSDEQ+VV
Sbjct: 61  KESMSHLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVV 120

Query: 121 QQV-EFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQV E+Y DFV+ DPYHFTLN+                LQ F DRVVDGIAA+FLALK+R
Sbjct: 121 QQVQEYYADFVSGDPYHFTLNMASNHLYMIPAVVDPSGLQRFSDRVVDGIAAVFLALKRR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSD AKRIA E +KLMYQ ES LFDFRR E SPLLLVIDRRDDP+TPLLNQWT
Sbjct: 181 PVIRYQRTSDTAKRIAHETAKLMYQHESALFDFRRTESSPLLLVIDRRDDPVTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQ-EVVLSSEQDSFYKANMYENFGDIGMNIKSW 300
           YQAMVHEL+G+QDNKVDLKSI    KDQQ EVVLSSEQD+F+K+NMYENFGDIGMNIK  
Sbjct: 241 YQAMVHELIGLQDNKVDLKSIGSLPKDQQVEVVLSSEQDAFFKSNMYENFGDIGMNIKRM 300

Query: 301 WMIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVS 360
              F ++ K         DMA+FVDNYPEY+KM GNVSKHVTLVTEMSK+VE RKLM VS
Sbjct: 301 VDDFQQVAKSNQNIQTVEDMARFVDNYPEYKKMQGNVSKHVTLVTEMSKLVEARKLMTVS 360

Query: 361 QTEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLA 420
           QTEQ+LACNGGQ AA+EAVT+LLNNES+SDIDRLRLVMLYALRYEKE+PVQLMQLFNKLA
Sbjct: 361 QTEQDLACNGGQGAAYEAVTDLLNNESVSDIDRLRLVMLYALRYEKENPVQLMQLFNKLA 420

Query: 421 SRSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQ 480
           SRS KYK GLVQFLLKQAGV+KRTGDL+GNRDLLNIARNMARGLKGVENVYTQHQPL+ Q
Sbjct: 421 SRSPKYKPGLVQFLLKQAGVEKRTGDLFGNRDLLNIARNMARGLKGVENVYTQHQPLLFQ 480

Query: 481 TMESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFIL 540
           TMESI +GRLRDVDYPFVG+HFQQGRPQEV+IF+VGGTTYEESR+VALQNAT SG+RFIL
Sbjct: 481 TMESITRGRLRDVDYPFVGDHFQQGRPQEVVIFMVGGTTYEESRSVALQNATNSGVRFIL 540

Query: 541 GGSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GG+ VLNS+RFLKDLEEAQRISRS + ++
Sbjct: 541 GGTAVLNSKRFLKDLEEAQRISRSGSHMV 569

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BLAST of PI0027353 vs. ExPASy Swiss-Prot
Match: P97390 (Vacuolar protein sorting-associated protein 45 OS=Mus musculus OX=10090 GN=Vps45 PE=1 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 8.8e-136
Identity = 264/560 (47.14%), Postives = 379/560 (67.68%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDIS-GMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSK 60
           M ++  V+ YI+KM++D   GMKVL++D +T  +VS+VY+QSE+LQKEV+L E +D  S+
Sbjct: 1   MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERID--SQ 60

Query: 61  SSESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDV 120
           + E M HLKA+CFLRPT EN++ L ++L  P++  Y ++FSN++ ++ +  LA++DEQ+V
Sbjct: 61  NREIMKHLKAICFLRPTKENVEYLIQELRRPKYSIYFIYFSNVISKSDVKSLAEADEQEV 120

Query: 121 VQQV-EFYGDFVAIDPYHFTLNIL---------QHFCDRVVDGIAALFLALKQRPVIRYQ 180
           V +V EFYGD++A++P+ F+LNIL              R   G+ AL L+LK+ P+IRYQ
Sbjct: 121 VAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQ 180

Query: 181 RTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAMVH 240
            +S+ AKR+  E  K +  +E  LF+FRR EV PLLL++DR DD +TPLLNQWTYQAMVH
Sbjct: 181 LSSEAAKRLG-ECVKQVISKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVH 240

Query: 241 ELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMIF-SK 300
           ELLGI +N++DL  +   SKD +EVVLS+E D FY  NMY NF +IG NIK+    F  K
Sbjct: 241 ELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKK 300

Query: 301 LPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQTEQEL 360
            PK         DM  FV+NYP+++KM G VSKHVT+V E+S++V ER L+ VS+ EQEL
Sbjct: 301 RPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQEL 360

Query: 361 ACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLASR--SA 420
           AC     +A + V  LL N  +++ D +RLVMLYAL YE+ S   L  L   L S+  + 
Sbjct: 361 ACQNDHSSALQNVKRLLQNPKVTEFDAVRLVMLYALHYERHSSNSLPGLIVDLRSKGVAE 420

Query: 421 KYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQTMES 480
           KY+  LV  +++  G   R  DL+  +D + I +   +GLKGVENVYTQHQP + +T++ 
Sbjct: 421 KYRK-LVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDH 480

Query: 481 IIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILGGSV 538
           +IKGRL++  YP++G    + RPQ++I+FI+GG TYEE+  V   N T  G+R +LGG+ 
Sbjct: 481 LIKGRLKENLYPYLGPSTLRDRPQDIIVFIIGGATYEEALTVYNLNRTTPGVRIVLGGTT 540

[Close]
BLAST of PI0027353 vs. ExPASy Swiss-Prot
Match: Q9NRW7 (Vacuolar protein sorting-associated protein 45 OS=Homo sapiens OX=9606 GN=VPS45 PE=1 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.5e-135
Identity = 265/562 (47.15%), Postives = 379/562 (67.44%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDIS-GMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSK 60
           M ++  V+ YI+KM++D   GMKVL++D +T  +VS+VY+QSE+LQKEV+L E +D  S+
Sbjct: 1   MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERID--SQ 60

Query: 61  SSESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDV 120
           + E M HLKA+CFLRPT EN+  + ++L  P++  Y ++FSN++ ++ +  LA++DEQ+V
Sbjct: 61  NREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEV 120

Query: 121 VQQV-EFYGDFVAIDPYHFTLNIL---------QHFCDRVVDGIAALFLALKQRPVIRYQ 180
           V +V EFYGD++A++P+ F+LNIL              R   G+ AL L+LK+ P+IRYQ
Sbjct: 121 VAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQ 180

Query: 181 RTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAMVH 240
            +S+ AKR+A E  K +  +E  LF+FRR EV PLLL++DR DD +TPLLNQWTYQAMVH
Sbjct: 181 LSSEAAKRLA-ECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVH 240

Query: 241 ELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMIF-SK 300
           ELLGI +N++DL  +   SKD +EVVLS+E D FY  NMY NF +IG NIK+    F  K
Sbjct: 241 ELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKK 300

Query: 301 LPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQTEQEL 360
            PK         DM  FV+NYP+++KM G VSKHVT+V E+S++V ER L+ VS+ EQEL
Sbjct: 301 KPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQEL 360

Query: 361 ACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKES----PVQLMQLFNKLASR 420
           AC     +A + +  LL N  +++ D  RLVMLYAL YE+ S    P  +M L NK    
Sbjct: 361 ACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLMMDLRNK--GV 420

Query: 421 SAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQTM 480
           S KY+  LV  +++  G   R  DL+  +D + I +   +GLKGVENVYTQHQP + +T+
Sbjct: 421 SEKYRK-LVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETL 480

Query: 481 ESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILGG 538
           + +IKGRL++  YP++G    + RPQ++I+F++GG TYEE+  V   N T  G+R +LGG
Sbjct: 481 DHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGG 540

[Close]
BLAST of PI0027353 vs. ExPASy Swiss-Prot
Match: O08700 (Vacuolar protein sorting-associated protein 45 OS=Rattus norvegicus OX=10116 GN=Vps45 PE=1 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 5.7e-135
Identity = 262/560 (46.79%), Postives = 378/560 (67.50%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDIS-GMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSK 60
           M ++  V+ YI+KM++D   GMKVL++D +T  +VS+VY+QSE+LQKEV+L E +D  S+
Sbjct: 1   MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERID--SQ 60

Query: 61  SSESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDV 120
           + E M HLKA+CFLRPT EN+  L ++L  P++  Y ++FSN++ ++ +  LA++DEQ+V
Sbjct: 61  NREIMKHLKAICFLRPTKENVDSLIQELRRPKYSIYFIYFSNVISKSDVKSLAEADEQEV 120

Query: 121 VQQV-EFYGDFVAIDPYHFTLNIL---------QHFCDRVVDGIAALFLALKQRPVIRYQ 180
           V +V EFYGD++A++P+ F+LNIL              R   G+ AL L+LK+ P+IRYQ
Sbjct: 121 VAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQ 180

Query: 181 RTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAMVH 240
            +S+ AKR+  E  K +  +E  LF+FRR EV PLLL++DR DD +TPLLNQWTYQAMVH
Sbjct: 181 LSSEAAKRLG-ECVKQVISKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVH 240

Query: 241 ELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMIF-SK 300
           ELLGI +N++DL  +   SKD +EVVLS+E D FY  NMY NF +IG NIK+    F  K
Sbjct: 241 ELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKK 300

Query: 301 LPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQTEQEL 360
            PK         DM  FV+NYP+++KM G VSKHVT+V E+S++V ER L+ VS+ EQEL
Sbjct: 301 RPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQEL 360

Query: 361 ACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLASR--SA 420
           AC     +A + V  LL N  +++ D +RLVMLYAL YE+ S   L  L   L S+  + 
Sbjct: 361 ACQNDHSSALQNVKRLLQNPKVTEFDAVRLVMLYALHYERHSSNSLPGLIVDLRSKGVAE 420

Query: 421 KYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQTMES 480
           KY+  LV  +++  G   R  DL+  +D + I +   +GLKGVENVYTQHQP + +T++ 
Sbjct: 421 KYRK-LVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDH 480

Query: 481 IIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILGGSV 538
           +IKG+L++  YP++G    + RPQ++I+F++GG TYEE+  V   N T  G+R +LGG+ 
Sbjct: 481 LIKGKLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTT 540

[Close]
BLAST of PI0027353 vs. ExPASy Swiss-Prot
Match: Q54GE3 (Vacuolar protein sorting-associated protein 45 OS=Dictyostelium discoideum OX=44689 GN=vps45 PE=1 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.4e-122
Identity = 244/562 (43.42%), Postives = 369/562 (65.66%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           M +I+ +++YINK+L +I GMKVL+LD +T  +VS+VY+QSE+LQKEVFL E ++    +
Sbjct: 6   MDVIASIQEYINKILTNIQGMKVLVLDKETAGIVSMVYTQSEILQKEVFLFEKIE---NT 65

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            E M H+K V F+RPT ENIQ +  +L +P+F +YHLFF+N + +  +  +A +DEQDVV
Sbjct: 66  KEKMLHMKGVYFIRPTQENIQSICDELKDPKFNKYHLFFTNTISKVSLDEIAKADEQDVV 125

Query: 121 QQV-EFYGDFVAIDPYHFTLNI----------LQHFCDRVVDGIAALFLALKQRPVIRYQ 180
            ++ E++GDF A++P  FTLN+           Q    RVVDG+ +  LALK++PVIRY 
Sbjct: 126 SEIQEYFGDFFAVNPDTFTLNLPGMLTKKSPRWQGDVGRVVDGLFSSLLALKKKPVIRYS 185

Query: 181 RTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAMVH 240
             SD  + +A++ ++ M  ++  LFDFRR +  PLLL++DR+DDP+TPLL+QWTYQAM+H
Sbjct: 186 SNSDTTRYLAEKITERM-NRDRDLFDFRR-QGEPLLLILDRKDDPITPLLHQWTYQAMIH 245

Query: 241 ELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMIFS-- 300
           ELL I +N+V L        + +EVVLS + D FYK N+Y+NFGD+G +IK     F   
Sbjct: 246 ELLTINNNRVSLAKAPGIKDELKEVVLSLDHDIFYKENLYKNFGDLGASIKDLVDQFQDK 305

Query: 301 -------KLPKDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQTEQELA 360
                  +   DM KF++NYP ++K    VSKHV+L+ E+++++    LM VS+ +QELA
Sbjct: 306 MNTNQNIQTIDDMKKFIENYPNFQKFSTTVSKHVSLMDELNRLISLDNLMEVSEVQQELA 365

Query: 361 CNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLASRSAKYK 420
           CN    + +  V  ++N+   +D D+L LV+LY++RYE     ++ +L  KL+S     K
Sbjct: 366 CNHDHNSIYNHVLEIVNDSKYTDRDKLVLVLLYSIRYEDG---RVWELKEKLSSIGIPPK 425

Query: 421 -TGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMA-RGLKGVENVYTQHQPLVVQTMESI 480
             GL+  L   AG   R GDL G +++ + AR++  RGL+GV N+YTQH+PL+   ++SI
Sbjct: 426 EIGLIDTLRGYAGASLREGDLLGTKNIFSFARSVVKRGLQGVSNIYTQHKPLLHDILDSI 485

Query: 481 IKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGI-RFILGGSV 540
           +K +L++  YP++     + RPQ+VIIF+VGG TYEE+  V   N+  +G+ R +LGG+ 
Sbjct: 486 LKNKLKETSYPYLSTTQSRERPQDVIIFMVGGITYEEALTVYTFNSLNTGVCRVVLGGTS 545

[Close]
BLAST of PI0027353 vs. ExPASy TrEMBL
Match: A0A5D3DJ93 (Vacuolar protein sorting-associated protein 45-like protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G00390 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.0e-285
Identity = 527/568 (92.78%), Postives = 533/568 (93.84%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
           SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV
Sbjct: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFYGDFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYGDFVAIDPYHFTLNMPSNHIYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHEL+GIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELIGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNSRRFLKDLEE QRISRSSAP+I
Sbjct: 541 GSVVLNSRRFLKDLEEGQRISRSSAPMI 568

[Close]
BLAST of PI0027353 vs. ExPASy TrEMBL
Match: A0A1S3B4C7 (vacuolar protein sorting-associated protein 45 homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485650 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.0e-285
Identity = 527/568 (92.78%), Postives = 533/568 (93.84%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
           SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV
Sbjct: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFYGDFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYGDFVAIDPYHFTLNMPSNHIYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHEL+GIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELIGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNSRRFLKDLEE QRISRSSAP+I
Sbjct: 541 GSVVLNSRRFLKDLEEGQRISRSSAPMI 568

[Close]
BLAST of PI0027353 vs. ExPASy TrEMBL
Match: A0A0A0KI85 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G338130 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 4.9e-283
Identity = 523/568 (92.08%), Postives = 530/568 (93.31%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSV YSQSELLQKEVFLVELVDAVSKS
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVAYSQSELLQKEVFLVELVDAVSKS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
           SE MYHLKAVCFLRPTSENIQLLRRQLA+PRFGEYHLFFSNILKETQIH+LADSDEQDVV
Sbjct: 61  SEPMYHLKAVCFLRPTSENIQLLRRQLASPRFGEYHLFFSNILKETQIHLLADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFYGDFVAIDPYHFTLN+                LQHFCDRVVDGI ALFLALKQR
Sbjct: 121 QQVVEFYGDFVAIDPYHFTLNMPSNHIYMIPAVVDPPSLQHFCDRVVDGITALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLV+DRRDDPLT LLNQWT
Sbjct: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVVDRRDDPLTALLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNAT SG+RFILG
Sbjct: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATTSGVRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNSRRFLKDLEEAQRISRSSAPVI
Sbjct: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 568

[Close]
BLAST of PI0027353 vs. ExPASy TrEMBL
Match: A0A6J1HH26 (vacuolar protein sorting-associated protein 45 homolog OS=Cucurbita moschata OX=3662 GN=LOC111463408 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.1e-278
Identity = 512/568 (90.14%), Postives = 526/568 (92.61%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQ+EVFLVELVD +S S
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQREVFLVELVDTISMS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            ESMYHLKAVCFLRPTSEN+QLLRR+LANPRFGEYHLFFSNILKETQIHILADSDEQDVV
Sbjct: 61  RESMYHLKAVCFLRPTSENVQLLRRELANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFY DFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYADFVAIDPYHFTLNMASNHRYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSD+A+RIAQE SKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQRTSDIARRIAQETSKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHELLGIQDNKVDLKSI KSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELLGIQDNKVDLKSIGKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNE+ISDIDR+RLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNENISDIDRVRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRD+DYPFVGNHFQQ RPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDMDYPFVGNHFQQARPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNS+RFLKDLEEAQRISRSSA VI
Sbjct: 541 GSVVLNSKRFLKDLEEAQRISRSSASVI 568

[Close]
BLAST of PI0027353 vs. ExPASy TrEMBL
Match: A0A6J1JQC3 (vacuolar protein sorting-associated protein 45 homolog OS=Cucurbita maxima OX=3661 GN=LOC111486867 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 5.3e-277
Identity = 508/568 (89.44%), Postives = 526/568 (92.61%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQ+EVFLVELVD +S S
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQREVFLVELVDTISMS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            ESMYHLKAVCFLRPTSEN+QLLRR+LANPRFGEYHLFFSNILKETQIHILADSDEQDVV
Sbjct: 61  RESMYHLKAVCFLRPTSENVQLLRRELANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFY DFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYADFVAIDPYHFTLNMSSNHRYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQ+TSD+A+RIAQE SKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQKTSDIARRIAQETSKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHELLGIQDNKVDLKSI KSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELLGIQDNKVDLKSIGKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSK+VEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKVVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQ+AAFEAVTNLLNNE+ISDIDR+RLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQMAAFEAVTNLLNNENISDIDRVRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
            SAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 CSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRD+DYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDMDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNS+RFLKDLEEAQRISRSSA +I
Sbjct: 541 GSVVLNSKRFLKDLEEAQRISRSSASLI 568

[Close]
BLAST of PI0027353 vs. NCBI nr
Match: XP_008441554.1 (PREDICTED: vacuolar protein sorting-associated protein 45 homolog isoform X1 [Cucumis melo] >KAA0058897.1 vacuolar protein sorting-associated protein 45-like protein isoform X1 [Cucumis melo var. makuwa] >TYK23726.1 vacuolar protein sorting-associated protein 45-like protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 989.9 bits (2558), Expect = 8.3e-285
Identity = 527/568 (92.78%), Postives = 533/568 (93.84%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
           SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV
Sbjct: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFYGDFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYGDFVAIDPYHFTLNMPSNHIYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHEL+GIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELIGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNSRRFLKDLEE QRISRSSAP+I
Sbjct: 541 GSVVLNSRRFLKDLEEGQRISRSSAPMI 568

[Close]
BLAST of PI0027353 vs. NCBI nr
Match: XP_038885382.1 (vacuolar protein sorting-associated protein 45 homolog [Benincasa hispida])

HSP 1 Score: 983.4 bits (2541), Expect = 7.8e-283
Identity = 525/568 (92.43%), Postives = 530/568 (93.31%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVD+VSKS
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDSVSKS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            ESMYHLKAVCFLRPTSENIQLLRRQL NPRFGEYHLFFSNILKETQIHILADSDEQDVV
Sbjct: 61  RESMYHLKAVCFLRPTSENIQLLRRQLVNPRFGEYHLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFY DFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYADFVAIDPYHFTLNMPSNHIYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNSRRFLKDLEEAQRISRSSA VI
Sbjct: 541 GSVVLNSRRFLKDLEEAQRISRSSATVI 568

[Close]
BLAST of PI0027353 vs. NCBI nr
Match: XP_004148814.1 (vacuolar protein sorting-associated protein 45 homolog isoform X1 [Cucumis sativus] >KGN47481.1 hypothetical protein Csa_019037 [Cucumis sativus])

HSP 1 Score: 983.0 bits (2540), Expect = 1.0e-282
Identity = 523/568 (92.08%), Postives = 530/568 (93.31%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSV YSQSELLQKEVFLVELVDAVSKS
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVAYSQSELLQKEVFLVELVDAVSKS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
           SE MYHLKAVCFLRPTSENIQLLRRQLA+PRFGEYHLFFSNILKETQIH+LADSDEQDVV
Sbjct: 61  SEPMYHLKAVCFLRPTSENIQLLRRQLASPRFGEYHLFFSNILKETQIHLLADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFYGDFVAIDPYHFTLN+                LQHFCDRVVDGI ALFLALKQR
Sbjct: 121 QQVVEFYGDFVAIDPYHFTLNMPSNHIYMIPAVVDPPSLQHFCDRVVDGITALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLV+DRRDDPLT LLNQWT
Sbjct: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVVDRRDDPLTALLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNAT SG+RFILG
Sbjct: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATTSGVRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNSRRFLKDLEEAQRISRSSAPVI
Sbjct: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 568

[Close]
BLAST of PI0027353 vs. NCBI nr
Match: XP_022963110.1 (vacuolar protein sorting-associated protein 45 homolog [Cucurbita moschata] >KAG6602160.1 Vacuolar protein sorting-associated protein 45-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 967.6 bits (2500), Expect = 4.4e-278
Identity = 512/568 (90.14%), Postives = 526/568 (92.61%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQ+EVFLVELVD +S S
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQREVFLVELVDTISMS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            ESMYHLKAVCFLRPTSEN+QLLRR+LANPRFGEYHLFFSNILKETQIHILADSDEQDVV
Sbjct: 61  RESMYHLKAVCFLRPTSENVQLLRRELANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFY DFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYADFVAIDPYHFTLNMASNHRYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSD+A+RIAQE SKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQRTSDIARRIAQETSKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHELLGIQDNKVDLKSI KSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELLGIQDNKVDLKSIGKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNE+ISDIDR+RLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNENISDIDRVRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRD+DYPFVGNHFQQ RPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDMDYPFVGNHFQQARPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNS+RFLKDLEEAQRISRSSA VI
Sbjct: 541 GSVVLNSKRFLKDLEEAQRISRSSASVI 568

[Close]
BLAST of PI0027353 vs. NCBI nr
Match: XP_023545203.1 (vacuolar protein sorting-associated protein 45 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.9 bits (2493), Expect = 2.9e-277
Identity = 511/568 (89.96%), Postives = 526/568 (92.61%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQ+EVFLVELVD +S S
Sbjct: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQREVFLVELVDTISMS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            ESMYHLKAVCFLRPTSEN+QLLRR+LANPRFGEY+LFFSNILKETQIHILADSDEQDVV
Sbjct: 61  RESMYHLKAVCFLRPTSENVQLLRRELANPRFGEYNLFFSNILKETQIHILADSDEQDVV 120

Query: 121 QQ-VEFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQ VEFY DFVAIDPYHFTLN+                LQHFCDRVVDGIAALFLALKQR
Sbjct: 121 QQVVEFYADFVAIDPYHFTLNMSSNHRYMIPAVVDPPSLQHFCDRVVDGIAALFLALKQR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSD+A+RIAQE SKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT
Sbjct: 181 PVIRYQRTSDIARRIAQETSKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWW 300
           YQAMVHELLGIQDNKVDLKSI KSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIK   
Sbjct: 241 YQAMVHELLGIQDNKVDLKSIGKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKKLV 300

Query: 301 MIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360
             F ++ K         DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ
Sbjct: 301 DDFQQIAKSNQNIQTIEDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVSQ 360

Query: 361 TEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLAS 420
           TEQELACNGGQVAAFEAVTNLLNNE+ISDIDR+RLVMLYALRYEKESPVQLMQLFNKLAS
Sbjct: 361 TEQELACNGGQVAAFEAVTNLLNNENISDIDRVRLVMLYALRYEKESPVQLMQLFNKLAS 420

Query: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQT 480
           RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPL+VQT
Sbjct: 421 RSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLLVQT 480

Query: 481 MESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFILG 540
           MESIIKGRLRD+DYPFVGNHFQQ RPQEVIIFIVGGTTYEESRAVALQNAT SGIRFILG
Sbjct: 481 MESIIKGRLRDMDYPFVGNHFQQARPQEVIIFIVGGTTYEESRAVALQNATTSGIRFILG 540

Query: 541 GSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GSVVLNS+RFLKDLEEAQRISRSSA VI
Sbjct: 541 GSVVLNSKRFLKDLEEAQRISRSSASVI 568

[Close]
BLAST of PI0027353 vs. TAIR 10
Match: AT1G77140.1 (vacuolar protein sorting 45 )

HSP 1 Score: 859.0 bits (2218), Expect = 2.1e-249
Identity = 450/569 (79.09%), Postives = 500/569 (87.87%), Query Frame = 0 

Query: 1   MVLISVVRDYINKMLQDISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKS 60
           MVL++ VRDYIN+MLQDISGMKVLILDS+TVS VS+VYSQSELLQKEVFLVE++D++S S
Sbjct: 1   MVLVTSVRDYINRMLQDISGMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDSISVS 60

Query: 61  SESMYHLKAVCFLRPTSENIQLLRRQLANPRFGEYHLFFSNILKETQIHILADSDEQDVV 120
            ESM HLKAV F+RPTS+NIQ LR QLANPRFGEYHLFFSN+LK+TQIHILADSDEQ+VV
Sbjct: 61  KESMSHLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVV 120

Query: 121 QQV-EFYGDFVAIDPYHFTLNI----------------LQHFCDRVVDGIAALFLALKQR 180
           QQV E+Y DFV+ DPYHFTLN+                LQ F DRVVDGIAA+FLALK+R
Sbjct: 121 QQVQEYYADFVSGDPYHFTLNMASNHLYMIPAVVDPSGLQRFSDRVVDGIAAVFLALKRR 180

Query: 181 PVIRYQRTSDVAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWT 240
           PVIRYQRTSD AKRIA E +KLMYQ ES LFDFRR E SPLLLVIDRRDDP+TPLLNQWT
Sbjct: 181 PVIRYQRTSDTAKRIAHETAKLMYQHESALFDFRRTESSPLLLVIDRRDDPVTPLLNQWT 240

Query: 241 YQAMVHELLGIQDNKVDLKSIAKSSKDQQ-EVVLSSEQDSFYKANMYENFGDIGMNIKSW 300
           YQAMVHEL+G+QDNKVDLKSI    KDQQ EVVLSSEQD+F+K+NMYENFGDIGMNIK  
Sbjct: 241 YQAMVHELIGLQDNKVDLKSIGSLPKDQQVEVVLSSEQDAFFKSNMYENFGDIGMNIKRM 300

Query: 301 WMIFSKLPK---------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVS 360
              F ++ K         DMA+FVDNYPEY+KM GNVSKHVTLVTEMSK+VE RKLM VS
Sbjct: 301 VDDFQQVAKSNQNIQTVEDMARFVDNYPEYKKMQGNVSKHVTLVTEMSKLVEARKLMTVS 360

Query: 361 QTEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLFNKLA 420
           QTEQ+LACNGGQ AA+EAVT+LLNNES+SDIDRLRLVMLYALRYEKE+PVQLMQLFNKLA
Sbjct: 361 QTEQDLACNGGQGAAYEAVTDLLNNESVSDIDRLRLVMLYALRYEKENPVQLMQLFNKLA 420

Query: 421 SRSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYTQHQPLVVQ 480
           SRS KYK GLVQFLLKQAGV+KRTGDL+GNRDLLNIARNMARGLKGVENVYTQHQPL+ Q
Sbjct: 421 SRSPKYKPGLVQFLLKQAGVEKRTGDLFGNRDLLNIARNMARGLKGVENVYTQHQPLLFQ 480

Query: 481 TMESIIKGRLRDVDYPFVGNHFQQGRPQEVIIFIVGGTTYEESRAVALQNATASGIRFIL 540
           TMESI +GRLRDVDYPFVG+HFQQGRPQEV+IF+VGGTTYEESR+VALQNAT SG+RFIL
Sbjct: 481 TMESITRGRLRDVDYPFVGDHFQQGRPQEVVIFMVGGTTYEESRSVALQNATNSGVRFIL 540

Query: 541 GGSVVLNSRRFLKDLEEAQRISRSSAPVI 543
           GG+ VLNS+RFLKDLEEAQRISRS + ++
Sbjct: 541 GGTAVLNSKRFLKDLEEAQRISRSGSHMV 569

[Close]
BLAST of PI0027353 vs. TAIR 10
Match: AT1G02010.1 (secretory 1A )

HSP 1 Score: 117.1 bits (292), Expect = 4.4e-26
Identity = 120/501 (23.95%), Postives = 227/501 (45.31%), Query Frame = 0 

Query: 17  DISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKSSESMYHLKAVCFLRPT 76
           D    K+LI+D  TV V+S     +++  + + LVE    + K  E M  + A+ F++P+
Sbjct: 41  DSKAWKILIMDRVTVKVMSQSCKMADITDQGISLVE---ELFKRREPMPGMDAIYFIQPS 100

Query: 77  SENIQLLRRQLA--NPRFGEYHLFFSN-ILKETQIHILADSDEQDVVQQV-EFYGDFVAI 136
            ENI +    ++   P + +  +FFS+ I KE   HI +DS     +  + E   ++  I
Sbjct: 101 KENIVMFLSDMSGREPLYRKAFIFFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPI 160

Query: 137 DPYHFTLNILQ----------------HFCDRVV-DGIAALFLALKQRPVIRYQRTSDVA 196
           D   F  +  Q                H C  ++   IA +F +LK+ P +RY+     A
Sbjct: 161 DNQGFLTDHEQALETLYAEDAENSRHFHICLNIMATRIATVFASLKELPFVRYRAAKSTA 220

Query: 197 KR--IAQEASKLMY---QQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAMVHE 256
            R  +  + +  ++    +   + +F + E    LL++DR  D + P++++WTY AM H+
Sbjct: 221 SRDLVPSKLAAAIWDCISKYKAIPNFPQTETCE-LLIVDRSVDQIAPIIHEWTYDAMCHD 280

Query: 257 LLGIQDNK--VDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMIFSK 316
           LL ++ NK  +++ S      +++E+VL  + D  +    + +  D    +      F+ 
Sbjct: 281 LLDMEGNKHVIEVPSKTGGPPEKKEIVL-EDHDPVWLELRHTHIADASERLHEKMTNFAS 340

Query: 317 LPK---------------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVS 376
             K               D+ K V   P+Y +    +S HV L  ++++I+ +  L  + 
Sbjct: 341 KNKAAQMRSRDGSELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRIIRDTGLRDLG 400

Query: 377 QTEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYAL----RYEKESPVQLMQLF 436
           Q EQ+L    G   A + +  L  N+  +  ++LRL+M+YA     ++E +  V+LMQL 
Sbjct: 401 QLEQDLVF--GDAGAKDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDKGVKLMQL- 460

Query: 437 NKLASRSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYT--QH 469
            +L+    K  + + Q +        ++G      D     +   +   G E  +   + 
Sbjct: 461 ARLSPVDMKVISNM-QLIAGSPENKAKSGSFSLKFDAGKTKQANRKDRSGEEETWQLFRF 520

[Close]
BLAST of PI0027353 vs. TAIR 10
Match: AT1G02010.2 (secretory 1A )

HSP 1 Score: 117.1 bits (292), Expect = 4.4e-26
Identity = 120/501 (23.95%), Postives = 227/501 (45.31%), Query Frame = 0 

Query: 17  DISGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKSSESMYHLKAVCFLRPT 76
           D    K+LI+D  TV V+S     +++  + + LVE    + K  E M  + A+ F++P+
Sbjct: 41  DSKAWKILIMDRVTVKVMSQSCKMADITDQGISLVE---ELFKRREPMPGMDAIYFIQPS 100

Query: 77  SENIQLLRRQLA--NPRFGEYHLFFSN-ILKETQIHILADSDEQDVVQQV-EFYGDFVAI 136
            ENI +    ++   P + +  +FFS+ I KE   HI +DS     +  + E   ++  I
Sbjct: 101 KENIVMFLSDMSGREPLYRKAFIFFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPI 160

Query: 137 DPYHFTLNILQ----------------HFCDRVV-DGIAALFLALKQRPVIRYQRTSDVA 196
           D   F  +  Q                H C  ++   IA +F +LK+ P +RY+     A
Sbjct: 161 DNQGFLTDHEQALETLYAEDAENSRHFHICLNIMATRIATVFASLKELPFVRYRAAKSTA 220

Query: 197 KR--IAQEASKLMY---QQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAMVHE 256
            R  +  + +  ++    +   + +F + E    LL++DR  D + P++++WTY AM H+
Sbjct: 221 SRDLVPSKLAAAIWDCISKYKAIPNFPQTETCE-LLIVDRSVDQIAPIIHEWTYDAMCHD 280

Query: 257 LLGIQDNK--VDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMIFSK 316
           LL ++ NK  +++ S      +++E+VL  + D  +    + +  D    +      F+ 
Sbjct: 281 LLDMEGNKHVIEVPSKTGGPPEKKEIVL-EDHDPVWLELRHTHIADASERLHEKMTNFAS 340

Query: 317 LPK---------------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKLMLVS 376
             K               D+ K V   P+Y +    +S HV L  ++++I+ +  L  + 
Sbjct: 341 KNKAAQMRSRDGSELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRIIRDTGLRDLG 400

Query: 377 QTEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYAL----RYEKESPVQLMQLF 436
           Q EQ+L    G   A + +  L  N+  +  ++LRL+M+YA     ++E +  V+LMQL 
Sbjct: 401 QLEQDLVF--GDAGAKDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDKGVKLMQL- 460

Query: 437 NKLASRSAKYKTGLVQFLLKQAGVDKRTGDLYGNRDLLNIARNMARGLKGVENVYT--QH 469
            +L+    K  + + Q +        ++G      D     +   +   G E  +   + 
Sbjct: 461 ARLSPVDMKVISNM-QLIAGSPENKAKSGSFSLKFDAGKTKQANRKDRSGEEETWQLFRF 520

[Close]
BLAST of PI0027353 vs. TAIR 10
Match: AT4G12120.1 (Sec1/munc18-like (SM) proteins superfamily )

HSP 1 Score: 116.3 bits (290), Expect = 7.6e-26
Identity = 118/506 (23.32%), Postives = 225/506 (44.47%), Query Frame = 0 

Query: 22  KVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKSSESMYHLKAVCFLRPTSENIQ 81
           KVL++D  TV ++S     SE+ Q+ + LVE+   ++K  + M  ++ + F++PT EN+ 
Sbjct: 44  KVLVMDKFTVKIMSSACKMSEITQEGISLVEV---ITKHRQPMTAMEVIYFIQPTEENVT 103

Query: 82  LLRRQLA--NPRFGEYHLFFSNILKETQIHILADSDEQDVVQQV----EFYGDFVAIDPY 141
                +   +P + +  +FFS+ +  + ++++    +   ++++    E   +++++D  
Sbjct: 104 AFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLI--KKDMRAMKRIGGLKEMNLEYISMDIQ 163

Query: 142 HFTLN----ILQHFCDR------------VVDGIAALFLALKQRPVIRYQRTSD------ 201
            F  N    + + FCD             V   IA +  +LK+ P +RY+          
Sbjct: 164 GFVTNNENALEELFCDDENHQRADACLNVVAKRIATVLASLKEYPFVRYRGAKALDATTM 223

Query: 202 ------VAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQAM 261
                 +  ++A      + + +  + DF + E    LL++DR  D + PL+++WTY AM
Sbjct: 224 TTYRELIPTKLAASVWNCLARYKQTIEDFPQTETCE-LLILDRSIDQIAPLIHEWTYDAM 283

Query: 262 VHELLGIQDNKVDLKSIAKS-SKDQQEVVLSSEQDSFY-----------KANMYENFGDI 321
            H+LL ++ NK   +  +K+  K +++ VL  E+DS +              ++E   + 
Sbjct: 284 CHDLLNMEGNKYTHEVPSKTGDKPEKKEVLLDEEDSIWVELRDAHIADASERLHEKMTNF 343

Query: 322 GMNIKSWWMIFSK------LPKDMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKL 381
               K+  +  S         KD+ K V   P+Y +    +S HV +   +++ + E+ L
Sbjct: 344 VSKNKAAQLKHSSKDFGDLSSKDLQKMVHALPQYSEQIDKLSLHVEIARTINRTIMEQGL 403

Query: 382 MLVSQTEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLF 441
             + Q EQ+L    G     + +  L  N  IS   +LRL+M+ A  Y K+   +  +  
Sbjct: 404 RDLGQLEQDLVF--GDAGRKDVIKFLSTNHIISHESKLRLIMIVAAIYPKKFEGEKGRKM 463

Query: 442 NKLASRSAKYKTGLVQF-LLKQAGVD---KRTGDLYGNRDLLNIARNMARGLKGVENVY- 470
            +LA  S      +    LL     +     TG      D+L   R   R   G    + 
Sbjct: 464 MELAKLSGDDVVAVNNMRLLGPVHTECKKSTTGSFPLKFDVLKTKRAARRDRVGETQTWQ 523

[Close]
BLAST of PI0027353 vs. TAIR 10
Match: AT1G12360.1 (Sec1/munc18-like (SM) proteins superfamily )

HSP 1 Score: 110.5 bits (275), Expect = 4.2e-24
Identity = 117/504 (23.21%), Postives = 220/504 (43.65%), Query Frame = 0 

Query: 19  SGMKVLILDSQTVSVVSVVYSQSELLQKEVFLVELVDAVSKSSESMYHLKAVCFLRPTSE 78
           S  KVLI+D  TV ++S     +++ Q+ V LVE    + +  + +  + A+ F++PT E
Sbjct: 41  STWKVLIMDKLTVKIMSYACKMADITQEGVSLVE---DIFRRRQPLPSMDAIYFIQPTKE 100

Query: 79  NIQLLRRQLA--NPRFGEYHLFFSN-ILKETQIHILADSDEQDVVQQV-EFYGDFVAIDP 138
           N+ +    ++  +P + +  +FFS+ + KE   HI  DS     +  + E   +F AID 
Sbjct: 101 NVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGALREMNLEFFAIDS 160

Query: 139 YHFTLN----ILQHFCDR------------VVDGIAALFLALKQRPVIRYQRTSD----- 198
             F  +    +   F D             +   IA +F +L++ P +RY+         
Sbjct: 161 QGFITDHERALEDLFGDEETSRKGDACLNVMASRIATVFASLREFPAVRYRAAKSLDAST 220

Query: 199 -------VAKRIAQEASKLMYQQESGLFDFRRMEVSPLLLVIDRRDDPLTPLLNQWTYQA 258
                  +  ++A      + + +  + +F + E    LL++DR  D + P++++WTY A
Sbjct: 221 MTTLRDLIPTKLAAGIWNCLAKHKQSIENFPQTETCE-LLILDRSIDQIAPVIHEWTYDA 280

Query: 259 MVHELLGIQDNK-VDLKSIAKSSKDQQEVVLSSEQDSFYKANMYENFGDIGMNIKSWWMI 318
           M H+LL ++ NK V +       + +++ VL  E D  +    + +  D    +      
Sbjct: 281 MCHDLLNMEGNKYVHVIPSKSGGQPEKKDVLLEEHDPIWLELRHAHIADASERLHDKMTN 340

Query: 319 FSKLPK----------------DMAKFVDNYPEYRKMHGNVSKHVTLVTEMSKIVEERKL 378
           F    K                D+ K V   P+Y +    +S HV +  +++ ++ E+ L
Sbjct: 341 FLSKNKAAQLQGKRDGAELSTRDLQKMVQALPQYSEQIDKLSLHVEIARKLNDLIREQGL 400

Query: 379 MLVSQTEQELACNGGQVAAFEAVTNLLNNESISDIDRLRLVMLYALRYEKESPVQLMQLF 438
             + Q EQ+L    G     + +  L   E  S   +LRL+M+ A  Y ++   +  Q  
Sbjct: 401 RELGQLEQDLVF--GDAGMKDVIKYLSTQEEASREGKLRLLMILATIYPEKFEGEKGQNL 460

Query: 439 NKLASRSAKYKTGLVQFLLKQAGVD--KRTGDLYGNRDLLNIARNMARGLKGVENVY--T 470
            KLA  S+   T +    L  + VD  K T   +  +  L+  +   R  +  E  +  +
Sbjct: 461 MKLAKLSSDDMTAVNNMSLLGSAVDAKKNTPGGFTLKFDLHKKKRAVRKERQEEAAWQLS 520

The following BLAST results are available for this feature:
BLAST of PI0027353 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O490482.9e-24879.09Vacuolar protein sorting-associated protein 45 homolog OS=Arabidopsis thaliana O... [more]
P973908.8e-13647.14Vacuolar protein sorting-associated protein 45 OS=Mus musculus OX=10090 GN=Vps45... [more]
Q9NRW72.5e-13547.15Vacuolar protein sorting-associated protein 45 OS=Homo sapiens OX=9606 GN=VPS45 ... [more]
O087005.7e-13546.79Vacuolar protein sorting-associated protein 45 OS=Rattus norvegicus OX=10116 GN=... [more]
Q54GE31.4e-12243.42Vacuolar protein sorting-associated protein 45 OS=Dictyostelium discoideum OX=44... [more]
BLAST of PI0027353 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3DJ934.0e-28592.78Vacuolar protein sorting-associated protein 45-like protein isoform X1 OS=Cucumi... [more]
A0A1S3B4C74.0e-28592.78vacuolar protein sorting-associated protein 45 homolog isoform X1 OS=Cucumis mel... [more]
A0A0A0KI854.9e-28392.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G338130 PE=3 SV=1[more]
A0A6J1HH262.1e-27890.14vacuolar protein sorting-associated protein 45 homolog OS=Cucurbita moschata OX=... [more]
A0A6J1JQC35.3e-27789.44vacuolar protein sorting-associated protein 45 homolog OS=Cucurbita maxima OX=36... [more]
BLAST of PI0027353 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008441554.18.3e-28592.78PREDICTED: vacuolar protein sorting-associated protein 45 homolog isoform X1 [Cu... [more]
XP_038885382.17.8e-28392.43vacuolar protein sorting-associated protein 45 homolog [Benincasa hispida][more]
XP_004148814.11.0e-28292.08vacuolar protein sorting-associated protein 45 homolog isoform X1 [Cucumis sativ... [more]
XP_022963110.14.4e-27890.14vacuolar protein sorting-associated protein 45 homolog [Cucurbita moschata] >KAG... [more]
XP_023545203.12.9e-27789.96vacuolar protein sorting-associated protein 45 homolog [Cucurbita pepo subsp. pe... [more]
BLAST of PI0027353 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G77140.12.1e-24979.09vacuolar protein sorting 45 [more]
AT1G02010.14.4e-2623.95secretory 1A [more]
AT1G02010.24.4e-2623.95secretory 1A [more]
AT4G12120.17.6e-2623.32Sec1/munc18-like (SM) proteins superfamily [more]
AT1G12360.14.2e-2423.21Sec1/munc18-like (SM) proteins superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043154Sec1-like, domain 1GENE3D3.40.50.2060coord: 3..126
e-value: 2.3E-34
score: 119.8
IPR001619Sec1-like proteinPFAMPF00995Sec1coord: 22..527
e-value: 8.2E-114
score: 382.0
IPR001619Sec1-like proteinPIRSFPIRSF005715VPS45_Sec1coord: 1..539
e-value: 3.5E-140
score: 466.2
IPR001619Sec1-like proteinPANTHERPTHR11679VESICLE PROTEIN SORTING-ASSOCIATEDcoord: 3..529
IPR027482Sec1-like, domain 2GENE3D3.40.50.1910coord: 143..529
e-value: 2.2E-119
score: 401.0
NoneNo IPR availableGENE3D1.25.40.60coord: 327..448
e-value: 2.2E-119
score: 401.0
NoneNo IPR availablePANTHERPTHR11679:SF3VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 45coord: 3..529
IPR043127Sec1-like, domain 3aGENE3D3.90.830.10Syntaxin Binding Protein 1; Chain A, domain 2coord: 221..325
e-value: 2.2E-119
score: 401.0
IPR036045Sec1-like superfamilySUPERFAMILY56815Sec1/munc18-like (SM) proteinscoord: 3..530

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027353.1PI0027353.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane