PI0027210 (gene) Melon (PI 482460) v1

Overview
NamePI0027210
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionARF guanine-nucleotide exchange factor GNL2
Locationchr05: 832367 .. 837651 (+)
RNA-Seq ExpressionPI0027210
SyntenyPI0027210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCCTAAATCTTCAACAACAATTCTCATCCTCAATATTCTCTCTTGTTCATGGACAAAAGATCATCCGATGACGAAGACGAAAAGGATTCTCCCAAATCTAAACGACGAGAGCTCGGTCTCTCCTGTATGTTAAACACCGAAGTCGGCGCCATCCTCGCCGTTATCCGCCGTCCACCTTCCGATTTAAACTCTCCTTATATCTCCACTATTGATGACACCTACGATTCCTCAATCCAACAATCCTTGAAATCCCTTCGAGCATTAATCTTCCACCCTCAACAAAAATGGCGAACTATCGACCCTTCCATCTACATCTCTCCTATTCTCGATGTTATACAAAGCGACGACATTCCCGCCGCGGCCACCGGCGTCGCTCTCTCTGCATTGCTCAAAATAATCAAGGTAGAAATCTTCGACGAGAAGACACCAGGTGCTAAAGACGCAATAAATTTGATAGTACTTGGAATCACAAATTGCAAACTTGAAAAAACTGACCTGGTAACCGAAGATGCCGTGATGATGAAGGTTCTACAAGTTCTGGCTGGATTGATGAACCACAGAGCTTCGTACCTGTTGAATGATCAATCTGTGTGTACAATTGTGAACACATGTTTCAATGTGGTCCAGCAATCGGCGAGTAGAGGGGATTTGCTGCAGAGGACGGCGAGGTACACGATGAATGAGCTGATACAGATAATATTTTCGAGGTTGCCGGAGATAGAGGTGAGAGACGGGGAGGAGTCGGAGTCGGATACGGAGGATGCGGATTTAGGAGGGAGTTTGGATTCAGGGTATGGGATAAGGTGTGTGATAGATGTGTTTCATTTTTTGTGTTCTTTGTTGAATGTGGTGGAAATGTTGGAGGTGGGGGATGGAGGGTTAGGATCGAGGACAGCGGATGAGGATGTTCAGTTGTTTGCTTTGGTGTTGATAAACTCGGCGGTGGAATTGAGTGGAGACGCCATTGGTAAACATCCAAAGCTTTTAAGGATGGTTCAGGATGATCTGTTTCATCATTTGATTCATTATGGAGCAGCTTCCAATCCATTAGTTTTGTCCATGATCTGCAGTACAGTTTTGAATATCTATCACTTCCTTCGAAGGTAAACACCCTTTTCCAGTTTTTAAAAATCCCCGCCTTAACTTATAACTACTTAGAAAATAATTTTCTATGAGTTTTCTACAAGATTAGTTTGACCTTCAACACTCACTGATGTCAACAAAAATAATCGTGACTAACTTTTTTTTTTGTTCTTCTAAAAGAGAGAACTTACTTGTAGATTAGTTTTTAGCAAATGAATTATTCAGGATTTATTTATTTTGAAGTTTAATAATATGGGGGAGTGATTTGAACTTGACCTCATGGTTTCAAAGTATAATATGCATCAACTCTTTTAAGTTAAGTTAAGATTAGAAGCATTGTAATAACTTATTCTTCATGAAAGTTTAGGAACTATTTTTTTAAAACTATATTTATTGAACGTTGAACTTTATTTTGGGTTAAAAGTATTTGGGCCTAGTCCAAAAAACAAAAGCCCAATTTCTAAAAGTTTCAAATATTAGAATATTGGAGATGCATAGAGTATATATATTGTAGAACCCTCTAGAATATATGAGAAAAATATGTAACCGTAAAGAATAGTAATTCTAGAAAGACATATATAGTCACTCAATTAGGAACGTACTGGCAAAATCGTAACTGTAAATTCATAAATTTAGAGGTATCAACTAGAGATATCTTAGAATTTCCATTAATGAGAAGCGATCAATAACACAAAAGGCAACTTTCTTTAACTCTATTTTTCAACCATATGGAAAAAGTACTTGAACAATTCACTTGAAGTTAATTTAAATTGATTAAAAAATTGTAACTTTTATGAAATGTAACAAAAAATAATTTCTAATTCTTCCTCCTACAAAATATTGCTGCAATTTTATAACTATGAGAGAAGTATAGTCATGCACAGAAGGCAAATATATCTCTCAACATTTCCCTAAAACATGAACTCAATACCTATAACAAATAAGGAGAAAGAATAAAGAGAAACCAAAGAACTAATATTTTTTCTATTTCATCAATATTGACAGGTTTGTTCGACTTCAATTAGAAGCATTCTTCATCTATGTGGCATTGAAATTAGCATCATTTGGGAACTCAACCCAAATCCAAGAAGTTGCACTTGAAGGAATCATAAACTTCTGTAGACAGTCTTCCTTCATTCTAGAATTCTATGTAAACTATGACTGTGATCCCCTTCGTTGGAATTTGTTCGAGGAGATCGGAAAGTTGCTATGTAAGCTCTCATTTCCTACAGGAAGTCCCTTAACCACTTTAAATATCCAAGCCTTCGAGGGACTAGTGATTGTGATTCACAACATTGCAGAAAAACTTGATAAACATAAAGAAGAAACTTGTGGGGGCAGCGGCAATTTAAGAGTATATCCAGCTCAAGTCGACGAATATATACCGTTTTGGGAAGAAAAATCTAAAGAAGACTTAGACTTGGAGGATTGGCTACGTTATGTAAGAGTAAGAAAGGCACAGAAGAAGAAGATATTGATTGCTGGTCACCATTTCAATAGAGATGAAAAGAAAGGCTTAGCATATTTGAAGCTTTCTCAATTGGTTTCTGATCCTCCGGACCCGAAAGCTTATGCTTACTTTTTCAGGTACACTCATGGATTGGACAAACAGTTTGTTGGTGAATATCTTGGTGACCCTGATCAGTTTCATGTAAAAGTGTTGGCTGAGTTTACAGAGACCTTTGAATTTACAGGCATGATTCTAGACACTGCTCTTAGAACTTACCTCGAGACGTTTCGTTTGCCGGGAGAAGCGCAAAAGATTCATCGCATATTAGAGGCCTTTTCAGAGAGATTTTATGAACTACAATCTTCAAATACCTTCGCGAGTAAAGATACAGTGTTTGTTCTTTGCTATTCTTTAATTATGTTGAATACTGATCAACATAATCCACAAGTGAAGAAAAAGATGACCGAAGACGAGTTCATCAGAAACAACAGAGAAATTAATGCAGGGAAGGATCTTCCTAGAGATTATTTATCTGAGCTTTTTCATTCGATTTCAAATAATGCAATTATACTCTCACCACAGTCAGGTTTGCAACTTGATATGAACCCTAGTAAATGGGTTGAATTGATGAATAGATCCAAGATTATACAACCTTTCATGTCGTGTGATTTCGACCCTCGCCTGGGAAGAGACATGTTTGGCTGCATTGCTGGTCCTTCAGTTGCATCTCTTGCAGCTTTCTTTGAGCATGCGGATGAAGATGAGATGCTGAATGAATGCATTGAAGGATTGTTTTCCATTACTAAGATAACACAGTATGGATTAGAGGACACGCTGGATGAACTGCTGGCAATGTTTTGTAAATTTACCACTCTATTGAACCCTTATGCATCTGCAGAGGAAACACTTTTTGTATTTAGTCATGATTTGAAGCCAAAACTGGCAACACTAGCAGTTTTCACGATTGCAAACAACTTCGGTGACACGATTCGAGGAGGTTGGAGAAACATTGTGGATTGCCTACTGAAACTCAAAAGACTTAAACTACTTCCCCAATCAGTTATTGACTTTGAAGTAGCTTCCACCTCATCAAATGATGTTGCAAGGTCAGATTCAGGTGTGATTTTCCCATCTCAAGATCCTAAATTTTGCACCCAGCAATCCTCTGGTATGGCCAGTCGGTTTTCGCAGTTTTTATCACTTGATAGTATGGAAGATTCCTTAACTCTCAACTTAAATGAATATGAACAAAATTTGAAGTTTGTCAAGCAATGTCGCATCGGGAACATCTTTAGCAACAGTGCAAACATACTTGATGAGGCTCTACTCAATCTTGGACGGTCCTTAATATTTGCCGCTGCTGGAAAAGGACAGAAGTTTAGCACACCAGTTGAAGAAGAAGAAACTGTCGGATTTTGTTGGGATCTAATTATAACGATGACCATGGCTAACATATACCGGTTCCAAGTTTTCTGGCCTAGCTTCCATGAGTATCTACAAACAGTTGTTCAATTTCCTCTTTTCTCTGCCATCCCATTTGCAGAAAAGGCCGTTTTAGGACTTTTCAAGGTATGTTTAACGCTCCTTTCGACATATCAACCTGACAAACTACCTGAGGAACTCATCTTCAAGTCCATAAATTTGATGTGGATGCTTGATAAGGAGATTCTTGATACTTGCTTTGAGTCCATAACACAATCAGTAAGCAAGATTCTTGTTGAATACCCAGCAAATCTCCAAAGCCAAATTGGTTGGAAGTCGCTTCTTCATTTGTTGTCAGCCACCGGTCGGCATCCCGAAACCTATGACCAAGGAGTCGAGACCTTGATCATGTTGATGTCTGATGCGTCACATATAACACGCACAAACTATACCTTCTGTATAGATTGCGCCTTTAGTTATGTTGCACTCAAGAATAGTCCGTTAGACAAGAACTTGAAAATCTTAGATTCATTATCAGATTCGGTAAATTTCTTGGTCCAATGGTACAGGCATTACTGTGCAGAATCAGGGAATAGTTTTAGTGTAGCAAGCAATGCAAGTAGCTCCTCCTTAGATGACAAAGGTTTAGGATCTTCTAATTTTGCCTTGACTTTATTTCTCAAACTAGGCGAAGCACTCCGAAAAACAAGCTTAGCTAGAAGAGAAGAGATAAGAAACCATGCCATTACATCTCTTAAGAAAAGCTTTGTGTTGGCAGAAGAATTGGATTTTCCCCCAACCAACTGTATTGGTTGTTTTAACAATATAATTTTTGCAATGGTAGATGATTTGCATGAAAAAATGTTGGAGTACTCTAGAAGAGATAATGCAGAGAGAGAGGCAAGAAGCATGGAGGGTACTTTAAAGATTTCAATGGAGCTCTTGACAGATGTTTACTTAGTATACTTGAAACAGATATCAGAAAGCCCTGGGTTCCGAACCTTTTGGCTTGGGGTATTGAGAAGAATGGATACCTGTATGAAGGCTGATCTAGGAAGTTATGGTGAATCAAGTTTGAAAGAGTTAGTCCCAGAATTATTGAGAAAAATAATCACAACTATGAGAGGAAAAGAGATATTGGTGAAGAAAGAAGGTGAAGATCTGTGGGAAATAACTTACATTCAGATCCAGTGGATTGCTCCTGGCATCAAAGACGAGCTTTTCCCAGAAGAATGCTTTTGAACATGTGACATATTACTTGTAAAATAATAGATTAACTTTAACCCACTGATAATTTACCTTTGTTTTTGCTCTTAGAAGGTAATCG

mRNA sequence

CCCTCCTAAATCTTCAACAACAATTCTCATCCTCAATATTCTCTCTTGTTCATGGACAAAAGATCATCCGATGACGAAGACGAAAAGGATTCTCCCAAATCTAAACGACGAGAGCTCGGTCTCTCCTGTATGTTAAACACCGAAGTCGGCGCCATCCTCGCCGTTATCCGCCGTCCACCTTCCGATTTAAACTCTCCTTATATCTCCACTATTGATGACACCTACGATTCCTCAATCCAACAATCCTTGAAATCCCTTCGAGCATTAATCTTCCACCCTCAACAAAAATGGCGAACTATCGACCCTTCCATCTACATCTCTCCTATTCTCGATGTTATACAAAGCGACGACATTCCCGCCGCGGCCACCGGCGTCGCTCTCTCTGCATTGCTCAAAATAATCAAGGTAGAAATCTTCGACGAGAAGACACCAGGTGCTAAAGACGCAATAAATTTGATAGTACTTGGAATCACAAATTGCAAACTTGAAAAAACTGACCTGGTAACCGAAGATGCCGTGATGATGAAGGTTCTACAAGTTCTGGCTGGATTGATGAACCACAGAGCTTCGTACCTGTTGAATGATCAATCTGTGTGTACAATTGTGAACACATGTTTCAATGTGGTCCAGCAATCGGCGAGTAGAGGGGATTTGCTGCAGAGGACGGCGAGGTACACGATGAATGAGCTGATACAGATAATATTTTCGAGGTTGCCGGAGATAGAGGTGAGAGACGGGGAGGAGTCGGAGTCGGATACGGAGGATGCGGATTTAGGAGGGAGTTTGGATTCAGGGTATGGGATAAGGTGTGTGATAGATGTGTTTCATTTTTTGTGTTCTTTGTTGAATGTGGTGGAAATGTTGGAGGTGGGGGATGGAGGGTTAGGATCGAGGACAGCGGATGAGGATGTTCAGTTGTTTGCTTTGGTGTTGATAAACTCGGCGGTGGAATTGAGTGGAGACGCCATTGGTAAACATCCAAAGCTTTTAAGGATGGTTCAGGATGATCTGTTTCATCATTTGATTCATTATGGAGCAGCTTCCAATCCATTAGTTTTGTCCATGATCTGCAGTACAGTTTTGAATATCTATCACTTCCTTCGAAGGTTTGTTCGACTTCAATTAGAAGCATTCTTCATCTATGTGGCATTGAAATTAGCATCATTTGGGAACTCAACCCAAATCCAAGAAGTTGCACTTGAAGGAATCATAAACTTCTGTAGACAGTCTTCCTTCATTCTAGAATTCTATGTAAACTATGACTGTGATCCCCTTCGTTGGAATTTGTTCGAGGAGATCGGAAAGTTGCTATGTAAGCTCTCATTTCCTACAGGAAGTCCCTTAACCACTTTAAATATCCAAGCCTTCGAGGGACTAGTGATTGTGATTCACAACATTGCAGAAAAACTTGATAAACATAAAGAAGAAACTTGTGGGGGCAGCGGCAATTTAAGAGTATATCCAGCTCAAGTCGACGAATATATACCGTTTTGGGAAGAAAAATCTAAAGAAGACTTAGACTTGGAGGATTGGCTACGTTATGTAAGAGTAAGAAAGGCACAGAAGAAGAAGATATTGATTGCTGGTCACCATTTCAATAGAGATGAAAAGAAAGGCTTAGCATATTTGAAGCTTTCTCAATTGGTTTCTGATCCTCCGGACCCGAAAGCTTATGCTTACTTTTTCAGGTACACTCATGGATTGGACAAACAGTTTGTTGGTGAATATCTTGGTGACCCTGATCAGTTTCATGTAAAAGTGTTGGCTGAGTTTACAGAGACCTTTGAATTTACAGGCATGATTCTAGACACTGCTCTTAGAACTTACCTCGAGACGTTTCGTTTGCCGGGAGAAGCGCAAAAGATTCATCGCATATTAGAGGCCTTTTCAGAGAGATTTTATGAACTACAATCTTCAAATACCTTCGCGAGTAAAGATACAGTGTTTGTTCTTTGCTATTCTTTAATTATGTTGAATACTGATCAACATAATCCACAAGTGAAGAAAAAGATGACCGAAGACGAGTTCATCAGAAACAACAGAGAAATTAATGCAGGGAAGGATCTTCCTAGAGATTATTTATCTGAGCTTTTTCATTCGATTTCAAATAATGCAATTATACTCTCACCACAGTCAGGTTTGCAACTTGATATGAACCCTAGTAAATGGGTTGAATTGATGAATAGATCCAAGATTATACAACCTTTCATGTCGTGTGATTTCGACCCTCGCCTGGGAAGAGACATGTTTGGCTGCATTGCTGGTCCTTCAGTTGCATCTCTTGCAGCTTTCTTTGAGCATGCGGATGAAGATGAGATGCTGAATGAATGCATTGAAGGATTGTTTTCCATTACTAAGATAACACAGTATGGATTAGAGGACACGCTGGATGAACTGCTGGCAATGTTTTGTAAATTTACCACTCTATTGAACCCTTATGCATCTGCAGAGGAAACACTTTTTGTATTTAGTCATGATTTGAAGCCAAAACTGGCAACACTAGCAGTTTTCACGATTGCAAACAACTTCGGTGACACGATTCGAGGAGGTTGGAGAAACATTGTGGATTGCCTACTGAAACTCAAAAGACTTAAACTACTTCCCCAATCAGTTATTGACTTTGAAGTAGCTTCCACCTCATCAAATGATGTTGCAAGGTCAGATTCAGGTGTGATTTTCCCATCTCAAGATCCTAAATTTTGCACCCAGCAATCCTCTGGTATGGCCAGTCGGTTTTCGCAGTTTTTATCACTTGATAGTATGGAAGATTCCTTAACTCTCAACTTAAATGAATATGAACAAAATTTGAAGTTTGTCAAGCAATGTCGCATCGGGAACATCTTTAGCAACAGTGCAAACATACTTGATGAGGCTCTACTCAATCTTGGACGGTCCTTAATATTTGCCGCTGCTGGAAAAGGACAGAAGTTTAGCACACCAGTTGAAGAAGAAGAAACTGTCGGATTTTGTTGGGATCTAATTATAACGATGACCATGGCTAACATATACCGGTTCCAAGTTTTCTGGCCTAGCTTCCATGAGTATCTACAAACAGTTGTTCAATTTCCTCTTTTCTCTGCCATCCCATTTGCAGAAAAGGCCGTTTTAGGACTTTTCAAGGTATGTTTAACGCTCCTTTCGACATATCAACCTGACAAACTACCTGAGGAACTCATCTTCAAGTCCATAAATTTGATGTGGATGCTTGATAAGGAGATTCTTGATACTTGCTTTGAGTCCATAACACAATCAGTAAGCAAGATTCTTGTTGAATACCCAGCAAATCTCCAAAGCCAAATTGGTTGGAAGTCGCTTCTTCATTTGTTGTCAGCCACCGGTCGGCATCCCGAAACCTATGACCAAGGAGTCGAGACCTTGATCATGTTGATGTCTGATGCGTCACATATAACACGCACAAACTATACCTTCTGTATAGATTGCGCCTTTAGTTATGTTGCACTCAAGAATAGTCCGTTAGACAAGAACTTGAAAATCTTAGATTCATTATCAGATTCGGTAAATTTCTTGGTCCAATGGTACAGGCATTACTGTGCAGAATCAGGGAATAGTTTTAGTGTAGCAAGCAATGCAAGTAGCTCCTCCTTAGATGACAAAGGTTTAGGATCTTCTAATTTTGCCTTGACTTTATTTCTCAAACTAGGCGAAGCACTCCGAAAAACAAGCTTAGCTAGAAGAGAAGAGATAAGAAACCATGCCATTACATCTCTTAAGAAAAGCTTTGTGTTGGCAGAAGAATTGGATTTTCCCCCAACCAACTGTATTGGTTGTTTTAACAATATAATTTTTGCAATGGTAGATGATTTGCATGAAAAAATGTTGGAGTACTCTAGAAGAGATAATGCAGAGAGAGAGGCAAGAAGCATGGAGGGTACTTTAAAGATTTCAATGGAGCTCTTGACAGATGTTTACTTAGTATACTTGAAACAGATATCAGAAAGCCCTGGGTTCCGAACCTTTTGGCTTGGGGTATTGAGAAGAATGGATACCTGTATGAAGGCTGATCTAGGAAGTTATGGTGAATCAAGTTTGAAAGAGTTAGTCCCAGAATTATTGAGAAAAATAATCACAACTATGAGAGGAAAAGAGATATTGGTGAAGAAAGAAGGTGAAGATCTGTGGGAAATAACTTACATTCAGATCCAGTGGATTGCTCCTGGCATCAAAGACGAGCTTTTCCCAGAAGAATGCTTTTGAACATGTGACATATTACTTGTAAAATAATAGATTAACTTTAACCCACTGATAATTTACCTTTGTTTTTGCTCTTAGAAGGTAATCG

Coding sequence (CDS)

ATGGACAAAAGATCATCCGATGACGAAGACGAAAAGGATTCTCCCAAATCTAAACGACGAGAGCTCGGTCTCTCCTGTATGTTAAACACCGAAGTCGGCGCCATCCTCGCCGTTATCCGCCGTCCACCTTCCGATTTAAACTCTCCTTATATCTCCACTATTGATGACACCTACGATTCCTCAATCCAACAATCCTTGAAATCCCTTCGAGCATTAATCTTCCACCCTCAACAAAAATGGCGAACTATCGACCCTTCCATCTACATCTCTCCTATTCTCGATGTTATACAAAGCGACGACATTCCCGCCGCGGCCACCGGCGTCGCTCTCTCTGCATTGCTCAAAATAATCAAGGTAGAAATCTTCGACGAGAAGACACCAGGTGCTAAAGACGCAATAAATTTGATAGTACTTGGAATCACAAATTGCAAACTTGAAAAAACTGACCTGGTAACCGAAGATGCCGTGATGATGAAGGTTCTACAAGTTCTGGCTGGATTGATGAACCACAGAGCTTCGTACCTGTTGAATGATCAATCTGTGTGTACAATTGTGAACACATGTTTCAATGTGGTCCAGCAATCGGCGAGTAGAGGGGATTTGCTGCAGAGGACGGCGAGGTACACGATGAATGAGCTGATACAGATAATATTTTCGAGGTTGCCGGAGATAGAGGTGAGAGACGGGGAGGAGTCGGAGTCGGATACGGAGGATGCGGATTTAGGAGGGAGTTTGGATTCAGGGTATGGGATAAGGTGTGTGATAGATGTGTTTCATTTTTTGTGTTCTTTGTTGAATGTGGTGGAAATGTTGGAGGTGGGGGATGGAGGGTTAGGATCGAGGACAGCGGATGAGGATGTTCAGTTGTTTGCTTTGGTGTTGATAAACTCGGCGGTGGAATTGAGTGGAGACGCCATTGGTAAACATCCAAAGCTTTTAAGGATGGTTCAGGATGATCTGTTTCATCATTTGATTCATTATGGAGCAGCTTCCAATCCATTAGTTTTGTCCATGATCTGCAGTACAGTTTTGAATATCTATCACTTCCTTCGAAGGTTTGTTCGACTTCAATTAGAAGCATTCTTCATCTATGTGGCATTGAAATTAGCATCATTTGGGAACTCAACCCAAATCCAAGAAGTTGCACTTGAAGGAATCATAAACTTCTGTAGACAGTCTTCCTTCATTCTAGAATTCTATGTAAACTATGACTGTGATCCCCTTCGTTGGAATTTGTTCGAGGAGATCGGAAAGTTGCTATGTAAGCTCTCATTTCCTACAGGAAGTCCCTTAACCACTTTAAATATCCAAGCCTTCGAGGGACTAGTGATTGTGATTCACAACATTGCAGAAAAACTTGATAAACATAAAGAAGAAACTTGTGGGGGCAGCGGCAATTTAAGAGTATATCCAGCTCAAGTCGACGAATATATACCGTTTTGGGAAGAAAAATCTAAAGAAGACTTAGACTTGGAGGATTGGCTACGTTATGTAAGAGTAAGAAAGGCACAGAAGAAGAAGATATTGATTGCTGGTCACCATTTCAATAGAGATGAAAAGAAAGGCTTAGCATATTTGAAGCTTTCTCAATTGGTTTCTGATCCTCCGGACCCGAAAGCTTATGCTTACTTTTTCAGGTACACTCATGGATTGGACAAACAGTTTGTTGGTGAATATCTTGGTGACCCTGATCAGTTTCATGTAAAAGTGTTGGCTGAGTTTACAGAGACCTTTGAATTTACAGGCATGATTCTAGACACTGCTCTTAGAACTTACCTCGAGACGTTTCGTTTGCCGGGAGAAGCGCAAAAGATTCATCGCATATTAGAGGCCTTTTCAGAGAGATTTTATGAACTACAATCTTCAAATACCTTCGCGAGTAAAGATACAGTGTTTGTTCTTTGCTATTCTTTAATTATGTTGAATACTGATCAACATAATCCACAAGTGAAGAAAAAGATGACCGAAGACGAGTTCATCAGAAACAACAGAGAAATTAATGCAGGGAAGGATCTTCCTAGAGATTATTTATCTGAGCTTTTTCATTCGATTTCAAATAATGCAATTATACTCTCACCACAGTCAGGTTTGCAACTTGATATGAACCCTAGTAAATGGGTTGAATTGATGAATAGATCCAAGATTATACAACCTTTCATGTCGTGTGATTTCGACCCTCGCCTGGGAAGAGACATGTTTGGCTGCATTGCTGGTCCTTCAGTTGCATCTCTTGCAGCTTTCTTTGAGCATGCGGATGAAGATGAGATGCTGAATGAATGCATTGAAGGATTGTTTTCCATTACTAAGATAACACAGTATGGATTAGAGGACACGCTGGATGAACTGCTGGCAATGTTTTGTAAATTTACCACTCTATTGAACCCTTATGCATCTGCAGAGGAAACACTTTTTGTATTTAGTCATGATTTGAAGCCAAAACTGGCAACACTAGCAGTTTTCACGATTGCAAACAACTTCGGTGACACGATTCGAGGAGGTTGGAGAAACATTGTGGATTGCCTACTGAAACTCAAAAGACTTAAACTACTTCCCCAATCAGTTATTGACTTTGAAGTAGCTTCCACCTCATCAAATGATGTTGCAAGGTCAGATTCAGGTGTGATTTTCCCATCTCAAGATCCTAAATTTTGCACCCAGCAATCCTCTGGTATGGCCAGTCGGTTTTCGCAGTTTTTATCACTTGATAGTATGGAAGATTCCTTAACTCTCAACTTAAATGAATATGAACAAAATTTGAAGTTTGTCAAGCAATGTCGCATCGGGAACATCTTTAGCAACAGTGCAAACATACTTGATGAGGCTCTACTCAATCTTGGACGGTCCTTAATATTTGCCGCTGCTGGAAAAGGACAGAAGTTTAGCACACCAGTTGAAGAAGAAGAAACTGTCGGATTTTGTTGGGATCTAATTATAACGATGACCATGGCTAACATATACCGGTTCCAAGTTTTCTGGCCTAGCTTCCATGAGTATCTACAAACAGTTGTTCAATTTCCTCTTTTCTCTGCCATCCCATTTGCAGAAAAGGCCGTTTTAGGACTTTTCAAGGTATGTTTAACGCTCCTTTCGACATATCAACCTGACAAACTACCTGAGGAACTCATCTTCAAGTCCATAAATTTGATGTGGATGCTTGATAAGGAGATTCTTGATACTTGCTTTGAGTCCATAACACAATCAGTAAGCAAGATTCTTGTTGAATACCCAGCAAATCTCCAAAGCCAAATTGGTTGGAAGTCGCTTCTTCATTTGTTGTCAGCCACCGGTCGGCATCCCGAAACCTATGACCAAGGAGTCGAGACCTTGATCATGTTGATGTCTGATGCGTCACATATAACACGCACAAACTATACCTTCTGTATAGATTGCGCCTTTAGTTATGTTGCACTCAAGAATAGTCCGTTAGACAAGAACTTGAAAATCTTAGATTCATTATCAGATTCGGTAAATTTCTTGGTCCAATGGTACAGGCATTACTGTGCAGAATCAGGGAATAGTTTTAGTGTAGCAAGCAATGCAAGTAGCTCCTCCTTAGATGACAAAGGTTTAGGATCTTCTAATTTTGCCTTGACTTTATTTCTCAAACTAGGCGAAGCACTCCGAAAAACAAGCTTAGCTAGAAGAGAAGAGATAAGAAACCATGCCATTACATCTCTTAAGAAAAGCTTTGTGTTGGCAGAAGAATTGGATTTTCCCCCAACCAACTGTATTGGTTGTTTTAACAATATAATTTTTGCAATGGTAGATGATTTGCATGAAAAAATGTTGGAGTACTCTAGAAGAGATAATGCAGAGAGAGAGGCAAGAAGCATGGAGGGTACTTTAAAGATTTCAATGGAGCTCTTGACAGATGTTTACTTAGTATACTTGAAACAGATATCAGAAAGCCCTGGGTTCCGAACCTTTTGGCTTGGGGTATTGAGAAGAATGGATACCTGTATGAAGGCTGATCTAGGAAGTTATGGTGAATCAAGTTTGAAAGAGTTAGTCCCAGAATTATTGAGAAAAATAATCACAACTATGAGAGGAAAAGAGATATTGGTGAAGAAAGAAGGTGAAGATCTGTGGGAAATAACTTACATTCAGATCCAGTGGATTGCTCCTGGCATCAAAGACGAGCTTTTCCCAGAAGAATGCTTTTGA

Protein sequence

MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDSSIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDADLGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPFWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLDELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLSLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKFSTPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLGLFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNSPLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHEKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCMKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDELFPEECF
Homology
BLAST of PI0027210 vs. ExPASy Swiss-Prot
Match: F4K2K3 (ARF guanine-nucleotide exchange factor GNL2 OS=Arabidopsis thaliana OX=3702 GN=GNL2 PE=2 SV=1)

HSP 1 Score: 1852.0 bits (4796), Expect = 0.0e+00
Identity = 913/1381 (66.11%), Postives = 1140/1381 (82.55%), Query Frame = 0

Query: 16   KSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTID-DTYDSSIQQSLKSLRALIF 75
            ++KR+ELG+SCMLNTEVGA+LAVIRRP   L+  Y+S  + D  DSS+QQSLKSLRALIF
Sbjct: 7    RAKRKELGISCMLNTEVGAVLAVIRRP---LSESYLSPQETDHCDSSVQQSLKSLRALIF 66

Query: 76   HPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAIN 135
            +PQQ WRTIDPS+Y+SP L+VIQSD+IPA+AT VALS++LKI+K+EIFDEKTPGAKDA+N
Sbjct: 67   NPQQDWRTIDPSVYLSPFLEVIQSDEIPASATAVALSSILKILKIEIFDEKTPGAKDAMN 126

Query: 136  LIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQ 195
             IV GIT+C+LEKTDLV+EDAVMM++LQVL G+M H +S LL DQ+VCTIVNTCF VVQQ
Sbjct: 127  SIVSGITSCRLEKTDLVSEDAVMMRILQVLTGIMKHPSSELLEDQAVCTIVNTCFQVVQQ 186

Query: 196  SASRGDLLQRTARYTMNELIQIIFSRLPEIEVR---DGEESESDTEDADLGGSLDSGYGI 255
            S  RGDLLQR  RYTM+ELIQIIFSRLP+ EVR    GE+SESDT++ D+ G    GYGI
Sbjct: 187  STGRGDLLQRNGRYTMHELIQIIFSRLPDFEVRGDEGGEDSESDTDEIDMSG----GYGI 246

Query: 256  RCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPK 315
            RC ID+FHFLCSLLNVVE++E  + G    TADEDVQ+FALVLINSA+ELSGDAIG+HPK
Sbjct: 247  RCCIDIFHFLCSLLNVVEVVENLE-GTNVHTADEDVQIFALVLINSAIELSGDAIGQHPK 306

Query: 316  LLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFIYVALKLAS 375
            LLRMVQDDLFHHLIHYGA+S+PLVLSMICS +LNIYHFLR+F+RLQLEAFF +V L++ +
Sbjct: 307  LLRMVQDDLFHHLIHYGASSSPLVLSMICSCILNIYHFLRKFMRLQLEAFFSFVLLRVTA 366

Query: 376  FGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLCKLSFPTGSPL 435
            F     +QEVALEG+INFCRQ +FI+E YVNYDCDP+  N+FEE GK+LC+ +FPT  PL
Sbjct: 367  FTGFLPLQEVALEGLINFCRQPAFIVEAYVNYDCDPMCRNIFEETGKVLCRHTFPTSGPL 426

Query: 436  TTLNIQAFEGLVIVIHNIAEKLDKHK----EETCGGSGNLRVYPAQVDEYIPFWEEKSKE 495
            T++ IQAFEGLVI+IHNIA+ +D+ +    EE    S  ++  P ++ EYIPFW +K KE
Sbjct: 427  TSIQIQAFEGLVILIHNIADNMDREEDEGNEEDDNNSNVIKPSPVEIHEYIPFWIDKPKE 486

Query: 496  DLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRY 555
              D E W+ ++RVRKAQK+K+ IA +HFNRDEKKGL YLK + LVSDP DP A A FFR+
Sbjct: 487  --DFETWVDHIRVRKAQKRKLAIAANHFNRDEKKGLEYLKYNYLVSDPLDPMALASFFRF 546

Query: 556  THGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHR 615
            T GLDK  +G+YLGDPD+ H+ VL  FT TFEFTGM LDTALRT+LE+FRLPGE+QKI R
Sbjct: 547  TPGLDKTMIGDYLGDPDELHLSVLRSFTHTFEFTGMNLDTALRTFLESFRLPGESQKIER 606

Query: 616  ILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREIN 675
            ++EAFSERFY+ QSS+ FASKDTV +LCYSLIMLNTDQHNPQV++KMTEDEFIRNNR IN
Sbjct: 607  MIEAFSERFYDQQSSDIFASKDTVHILCYSLIMLNTDQHNPQVRRKMTEDEFIRNNRAIN 666

Query: 676  AGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSCDFDPR 735
            AG DLP++YLSELF SI+ NA  LS  SG  ++MNP++W+ELMNR+K  QPF  C FD R
Sbjct: 667  AGNDLPKEYLSELFQSIATNAFALSTHSG-PVEMNPNRWIELMNRTKTTQPFSLCQFDRR 726

Query: 736  LGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLDELLAMFC 795
            +GRDMF  IAGPS+A+++AFFEH+D+DE+L+EC++ + SI ++ QYGLED LDEL+A FC
Sbjct: 727  IGRDMFATIAGPSIAAVSAFFEHSDDDEVLHECVDAMISIARVAQYGLEDILDELIASFC 786

Query: 796  KFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRL 855
            KFTTLLNPY + EETLF FSHD+KP++ATLAVFT+AN FGD+IRGGWRNIVDCLLKL++L
Sbjct: 787  KFTTLLNPYTTPEETLFAFSHDMKPRMATLAVFTLANTFGDSIRGGWRNIVDCLLKLRKL 846

Query: 856  KLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLSLDSMEDS 915
            +LLPQSVI+FE+     N  + SD   +  SQD KF  +Q S +  RFS FL+LD++E+S
Sbjct: 847  QLLPQSVIEFEI--NEENGGSESDMNNV-SSQDTKFNRRQGSSLMGRFSHFLALDNVEES 906

Query: 916  LTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKFSTPVEEE 975
            + L ++E+EQNLK +KQCRIG IFS S+ + D A+LNLGRSLI+AAAGKGQKFST +EEE
Sbjct: 907  VALGMSEFEQNLKVIKQCRIGQIFSKSSVLPDVAVLNLGRSLIYAAAGKGQKFSTAIEEE 966

Query: 976  ETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLGLFKVCLT 1035
            ETV FCWDLIIT+ ++N++RF +FWPS+HEYL  V  FPLFS IPF EK + GLF+VC+ 
Sbjct: 967  ETVKFCWDLIITIALSNVHRFNMFWPSYHEYLLNVANFPLFSPIPFVEKGLPGLFRVCIK 1026

Query: 1036 LLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQSQIGWKS 1095
            +L++   D LPEELIF+S+ +MW +DKEI++TC+++IT+ VSKI+++Y ANL + IGWKS
Sbjct: 1027 ILASNLQDHLPEELIFRSLTIMWKIDKEIIETCYDTITEFVSKIIIDYSANLHTNIGWKS 1086

Query: 1096 LLHLLSATGRHPETYDQGVETLIMLMS-DASHITRTNYTFCIDCAFSYVALKNSPLDKNL 1155
            +L LLS  GRHPET +Q V+ LI LMS +ASH+++++Y +CIDCAFS+VAL+NS ++KNL
Sbjct: 1087 VLQLLSLCGRHPETKEQAVDALIGLMSFNASHLSQSSYAYCIDCAFSFVALRNSSVEKNL 1146

Query: 1156 KILDSLSDSVNFLVQWYRHYCAESGNSFSVASN-ASSSSLDDKGLGSSNFALTLFLKLGE 1215
            KILD ++DSV  LV+WY+    ++ NS+S ASN +SSSS+++  L   NF   LFLKL E
Sbjct: 1147 KILDLMADSVTMLVKWYKTASTDTANSYSPASNTSSSSSMEENNLRGVNFVHHLFLKLSE 1206

Query: 1216 ALRKTSLARREEIRNHAITSLKKSFVLA-EELDFPPTNCIGCFNNIIFAMVDDLHEKMLE 1275
            A RKT+LARREEIRN A+TSL+KSF +  E+L F P+ CI C +++IF  +DDLHEK+L+
Sbjct: 1207 AFRKTTLARREEIRNRAVTSLEKSFTMGHEDLGFTPSGCIYCIDHVIFPTIDDLHEKLLD 1266

Query: 1276 YSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCMKAD 1335
            YSRR+NAERE RSMEGTLKI+M++L +V+LVYL+QI ES  FRTFWLGVLRRMDTCMKAD
Sbjct: 1267 YSRRENAEREMRSMEGTLKIAMKVLMNVFLVYLEQIVESAEFRTFWLGVLRRMDTCMKAD 1326

Query: 1336 LGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDELFPE 1386
            LG YG++ L+E+VPELL  +I TM+ KEILV+KE +DLWEITYIQIQWIAP +KDELFP+
Sbjct: 1327 LGEYGDNKLQEVVPELLTTMIGTMKEKEILVQKEDDDLWEITYIQIQWIAPALKDELFPD 1373

BLAST of PI0027210 vs. ExPASy Swiss-Prot
Match: Q42510 (ARF guanine-nucleotide exchange factor GNOM OS=Arabidopsis thaliana OX=3702 GN=GN PE=1 SV=1)

HSP 1 Score: 1049.7 bits (2713), Expect = 3.0e-305
Identity = 597/1461 (40.86%), Postives = 892/1461 (61.05%), Query Frame = 0

Query: 4    RSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDSSIQ 63
            ++ ++E E            L+CM++TE+ A+LAV+RR        Y+S  DD  + S+ 
Sbjct: 11   KAIEEEPEDFECTDSSNTTTLACMIDTEIAAVLAVMRRNVR-WGGRYMSG-DDQLEHSLI 70

Query: 64   QSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFD 123
            QSLK+LR  +F   Q W TI P +Y+ P LDVI+SD+  A  T +ALS++ KI+ + + D
Sbjct: 71   QSLKALRKQVFSWNQPWHTISPMLYLQPFLDVIRSDETGAPITSIALSSVYKILNLNVID 130

Query: 124  EKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCT 183
            + T   +DA++L+V  +T+C+ E TD  +E+ V+MK+LQVL   M ++AS +L++Q VCT
Sbjct: 131  QNTANIEDAMHLVVDSVTSCRFEVTDPASEEVVLMKILQVLLACMKNKASVMLSNQHVCT 190

Query: 184  IVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIE------------------ 243
            +VNTCF VV Q+  +G+LLQR AR+TM+EL++ IFS LP++E                  
Sbjct: 191  VVNTCFRVVHQAGMKGELLQRVARHTMHELVRCIFSHLPDVERTETTLVNRAGSIKQEKA 250

Query: 244  ------------VRDGE-ESESDTE---------------DADLG-GS-----------L 303
                        V DG   SE D E               D  +G GS           +
Sbjct: 251  GVDSDYAIVSKPVEDGNANSEYDVENSMATFATGAQSLMDDGPVGPGSRKPASPYDLHIM 310

Query: 304  DSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRT----ADEDVQLFALVLINSAVEL 363
               YG+  ++++FHFLCSLLNVVE +     G+GSR+     DEDV LFAL LINSA+EL
Sbjct: 311  TEPYGVPSMVEIFHFLCSLLNVVEHV-----GMGSRSNTIAFDEDVPLFALNLINSAIEL 370

Query: 364  SGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAF 423
             G +I  HP+LL ++QD+LF +L+ +G + +PL+LSM+CS VLN+Y  LR  ++LQLEAF
Sbjct: 371  GGSSIRHHPRLLSLIQDELFRNLMQFGLSMSPLILSMVCSIVLNLYQHLRTELKLQLEAF 430

Query: 424  FIYVALKLA--SFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 483
            F  V L+LA   +G S Q QEVA+E ++NFCRQ SF++E Y N DCD    N+FEE+  L
Sbjct: 431  FSCVILRLAQGKYGPSYQQQEVAMEALVNFCRQKSFMVEMYANLDCDITCSNVFEELSNL 490

Query: 484  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 543
            L K +FP   PL+ ++I A +GL+ VI  +AE++         G   L + P  +DEY P
Sbjct: 491  LSKSTFPVNCPLSAMHILALDGLIAVIQGMAERIS-------NGLTGLDLGPVHLDEYTP 550

Query: 544  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPK 603
            FW  K     D   W+ +VR RK  K++++I   HFNRD KKGL +L+ + L+ D  DP+
Sbjct: 551  FWMVKCDNYSDPNHWVSFVRRRKYIKRRLMIGADHFNRDPKKGLEFLQGTHLLPDKLDPQ 610

Query: 604  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 663
            + A FFRYT GLDK  VG++LG+ D+F V+VL EF  TF+F  M LDTALR +LETFRLP
Sbjct: 611  SVACFFRYTAGLDKNLVGDFLGNHDEFCVQVLNEFAGTFDFQYMNLDTALRLFLETFRLP 670

Query: 664  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 723
            GE+QKI R+LEAFSER+Y +QS    A+KD   VL YS+IMLNTDQHN QVKKKMTE++F
Sbjct: 671  GESQKIQRVLEAFSERYY-MQSPEILANKDAALVLSYSIIMLNTDQHNVQVKKKMTEEDF 730

Query: 724  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQL-DMNPSKWVELMNRSKIIQP 783
            IRNNR IN G DLPR++LSELFHSI NN I  +P+ G    +M PS+W++LM++SK   P
Sbjct: 731  IRNNRHINGGNDLPREFLSELFHSICNNEIRTTPEQGAGFPEMTPSRWIDLMHKSKKTAP 790

Query: 784  FMSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQ-YGLED 843
            ++  D    L  DMF  ++GP++A+++  F+HA+ +++   CI+G  +I KI+  + LED
Sbjct: 791  YILADSRAYLDHDMFAIMSGPTIAAISVVFDHAEHEDVYQTCIDGFLAIAKISACHHLED 850

Query: 844  TLDELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNI 903
             LD+L+   CKFTTLLNP +S +E +  F  D K ++AT+ +FTIAN +GD IR GWRNI
Sbjct: 851  VLDDLVVSLCKFTTLLNP-SSVDEPVLAFGDDAKARMATITIFTIANKYGDYIRTGWRNI 910

Query: 904  VDCLLKLKRLKLLPQSVI-DFEVASTSSND------VARSDSGVIFPSQDPKFCTQQSSG 963
            +DC+L+L +L LLP  V  D    S  S++      +A S S     S       ++SSG
Sbjct: 911  LDCILRLHKLGLLPARVASDAADESEHSSEQGQGKPLANSLSSAHLQSMG---TPRRSSG 970

Query: 964  MASRFSQFLSLDSME---DSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGR 1023
            +  RFSQ LSLD+ E         L  +++ L+ +++C I +IF+ S  +  E+LL L R
Sbjct: 971  LMGRFSQLLSLDTEEPRSQPTEQQLAAHQRTLQTIQKCHIDSIFTESKFLQAESLLQLAR 1030

Query: 1024 SLIFAAAGKGQKFSTPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPL 1083
            +LI+ AAG+ QK ++  E+E+T  FC +L+I +T+ N  R  + W   +E++ T+ Q  +
Sbjct: 1031 ALIW-AAGRPQKGTSSPEDEDTAVFCLELLIAITLNNRDRIVLLWQGVYEHIATIAQSTV 1090

Query: 1084 FSAIPFAEKAVLGLFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQS 1143
                   +KA+ GL ++C  LL     + L +EL+ +S+ L+  LD  + D   E I   
Sbjct: 1091 MPC-NLVDKAIFGLLRICQRLLP--YKESLADELL-RSLQLVLKLDARVADAYCEQIAIE 1150

Query: 1144 VSKILVEYPANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFC 1203
            VS+++     +++SQ GW+++  LLS T RHPE  + G + +  +MS+ +H+   NY  C
Sbjct: 1151 VSRLVKANANHIRSQAGWRTITSLLSITARHPEASESGFDAVSFVMSEGTHLYPANYVLC 1210

Query: 1204 IDCAFSYVALKNSPLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDD 1263
            +D A  +   +    +++++ LD + DS+ FL +W          + S   N      +D
Sbjct: 1211 VDAARQFAESRVGQSERSIRALDLMGDSLEFLAKW----------ALSAKENMGE---ED 1270

Query: 1264 KGLGSSNFALTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCF 1323
             G  S +    ++L+L + LRK  L +RE++RNHA+ SL+K     + ++   +    CF
Sbjct: 1271 FGKMSQDIG-EMWLRLVQGLRKVCLDQREDVRNHALQSLQKCLGGVDGINLAHSMWSQCF 1330

Query: 1324 NNIIFAMVDDLHEKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFR 1383
            + +IF ++DDL    LE +    ++++ R+MEGTL ++++LL+ V+L  L+++S+   F 
Sbjct: 1331 DKVIFTVLDDL----LEIAA--GSQKDYRNMEGTLLLAIKLLSKVFLQQLQELSQLSTFC 1390

Query: 1384 TFWLGVLRRMDTCMKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKE---GEDLWE 1386
              WLGVL RM+  MK  +       L+E VPELL+ I+  M+ K +L+++    G+ LWE
Sbjct: 1391 KLWLGVLTRMEKYMKVKVRGKKSDKLQESVPELLKNILLVMKTKGVLLQRSALGGDSLWE 1427

BLAST of PI0027210 vs. ExPASy Swiss-Prot
Match: Q9FLY5 (ARF guanine-nucleotide exchange factor GNL1 OS=Arabidopsis thaliana OX=3702 GN=GNL1 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 3.1e-270
Identity = 544/1447 (37.60%), Postives = 848/1447 (58.60%), Query Frame = 0

Query: 17   SKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDSSIQQSLKSLRALIFHP 76
            SK  +  ++ M+N+E+GA+LAV+RR        YI+  DD  + S+  SLK LR  IF  
Sbjct: 22   SKPSKGAVASMINSEIGAVLAVMRRNVR-WGVRYIAD-DDQLEHSLIHSLKELRKQIFSW 81

Query: 77   QQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLI 136
            Q  W+ +DP +YI P LDVI SD+  A  TGVALS++ KI+ +E+F  +T    +A+++I
Sbjct: 82   QSNWQYVDPRLYIQPFLDVILSDETGAPITGVALSSVYKILTLEVFTLETVNVGEAMHII 141

Query: 137  VLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSA 196
            V  + +C+ E TD  +E+ V+MK+LQVL   +  +AS  L++Q +CTIVNTC  VV QS+
Sbjct: 142  VDAVKSCRFEVTDPASEEVVLMKILQVLLACVKSKASNGLSNQDICTIVNTCLRVVHQSS 201

Query: 197  SRGDLLQRTARYTMNELIQIIFSRL----------------------------------- 256
            S+ +LLQR AR+TM+ELI+ IFS+L                                   
Sbjct: 202  SKSELLQRIARHTMHELIRCIFSQLPFISPLANECELHVDNKVGTVDWDPNSGEKRVENG 261

Query: 257  ----------------------PEIEVRDGEESESDTEDADLGGSLDSG----YGIRCVI 316
                                  PE ++R+ E+    ++D +   + ++     YGI C++
Sbjct: 262  NIASISDTLGTDKDDPSSEMVIPETDLRNDEKKTEVSDDLNAAANGENAMMAPYGIPCMV 321

Query: 317  DVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRM 376
            ++FHFLC+LLNV E  EV          DEDV LFAL LINSA+EL G +  +HPKLL +
Sbjct: 322  EIFHFLCTLLNVGENGEVNSRS-NPIAFDEDVPLFALGLINSAIELGGPSFREHPKLLTL 381

Query: 377  VQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFIYVALKLA--SFG 436
            +QDDLF +L+ +G + +PL+LS +CS VLN+Y  LR  +++QLEAFF YV L++A    G
Sbjct: 382  IQDDLFCNLMQFGMSMSPLILSTVCSIVLNLYLNLRTELKVQLEAFFSYVLLRIAQSKHG 441

Query: 437  NSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLCKLSFPTGSPLTT 496
            +S Q QEVA+E +++ CRQ +FI E + N+DCD    N+FE++  LL K +FP   PL+ 
Sbjct: 442  SSYQQQEVAMEALVDLCRQHTFIAEVFANFDCDITCSNVFEDVSNLLSKNAFPVNGPLSA 501

Query: 497  LNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPFWEEKSKEDLDLED 556
            ++I A +GL+ ++  +AE++ +    +          P   + Y  FW  + +   D   
Sbjct: 502  MHILALDGLISMVQGMAERVGEELPAS--------DVPTHEERYEEFWTVRCENYGDPNF 561

Query: 557  WLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRYTHGLDK 616
            W+ +VR  K  KKK+++    FNRD  KGL YL+   L+ +  DPK+ A FFRYT GLDK
Sbjct: 562  WVPFVRKVKHIKKKLMLGADRFNRDPNKGLQYLQGVHLLPEKLDPKSVACFFRYTCGLDK 621

Query: 617  QFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFS 676
              +G++LG+ DQF ++VL EF +TF+F  M L TALR ++ TF+L GEAQKIHR+LEAFS
Sbjct: 622  NVMGDFLGNHDQFCIQVLHEFAKTFDFQNMNLATALRLFVGTFKLSGEAQKIHRVLEAFS 681

Query: 677  ERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREINAGKDLP 736
            ER+YE QS +    KD  FVL YS+I+LNTDQHN QVK +MTE++FIRNNR IN G DLP
Sbjct: 682  ERYYE-QSPHILIDKDAAFVLAYSIILLNTDQHNAQVKTRMTEEDFIRNNRTINGGADLP 741

Query: 737  RDYLSELFHSISNNAIILSPQ--SGLQLDMNPSKWVELMNRSKIIQPFMSCDFDPRLGRD 796
            R+YLSE++HSI ++ I +     +G QL M  S+W+ ++ +SK   P++ CD    L RD
Sbjct: 742  REYLSEIYHSIRHSEIQMDEDKGTGFQL-MTASRWISVIYKSKETSPYIQCDAASHLDRD 801

Query: 797  MFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKIT-QYGLEDTLDELLAMFCKFT 856
            MF  ++GP++A+ +  FE A+++++L  CI+GL +I K++  Y L   LD+L+   CKFT
Sbjct: 802  MFYIVSGPTIAATSVVFEQAEQEDVLRRCIDGLLAIAKLSAYYHLNSVLDDLVVSLCKFT 861

Query: 857  TLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLL 916
                P  SA+E + V   D + ++AT AVF IAN +GD I  GW+NI++C+L L +L +L
Sbjct: 862  PFFAP-LSADEAVLVLGEDARARMATEAVFLIANKYGDYISAGWKNILECVLSLNKLHIL 921

Query: 917  PQSVI-----DFEVASTSSNDVARSDSGVIFPSQ-DPKFCTQQSSGMASRFSQFLSLDSM 976
            P  +      D E+++++      S + V   SQ  P    ++SS    RF   LS DS 
Sbjct: 922  PDHIASDAADDPELSTSNLEQEKPSANPVPVVSQSQPSAMPRKSSSFIGRF--LLSFDSE 981

Query: 977  EDSLTLNLNE---YEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKFS 1036
            E     +  E   Y+     VK C I +IFS+S  +  E+L  L  SLI  A+GK     
Sbjct: 982  ETKPLPSEEELAAYKHARGIVKDCHIDSIFSDSKFLQAESLQQLVNSLI-RASGK----- 1041

Query: 1037 TPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLGL 1096
                +E +  FC +L+I +T+ N  R  + WP+ +E++  +VQ  L +     EKAV G+
Sbjct: 1042 ----DEASSVFCLELLIAVTLNNRDRILLIWPTVYEHILGIVQLTL-TPCTLVEKAVFGV 1101

Query: 1097 FKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQS 1156
             K+C  LL     + L +EL+ KS+ L+  L  ++ D   E I Q V +++    ++++S
Sbjct: 1102 LKICQRLLP--YKENLTDELL-KSLQLVLKLKAKVADAYCERIAQEVVRLVKANASHVRS 1161

Query: 1157 QIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNSP 1216
            + GW++++ LLS T RHPE  + G E L  +MS+ +H+  +NY  C+D A  +   +   
Sbjct: 1162 RTGWRTIISLLSITARHPEASEAGFEALRFIMSEGAHLLPSNYELCLDAASHFAESRVGE 1221

Query: 1217 LDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLFL 1276
            +D+++  +D +S+SV  L +W +    E+ NS    ++A     +D G         ++L
Sbjct: 1222 VDRSISAIDLMSNSVFCLARWSQ----EAKNSIG-ETDAMMKLSEDIG--------KMWL 1281

Query: 1277 KLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHEK 1336
            KL + L+K  L +R+E+RNHAI+ L+++   A+ +  P      CF++ +F ++DD    
Sbjct: 1282 KLVKNLKKVCLDQRDEVRNHAISMLQRAIAGADGIMLPQPLWFQCFDSAVFILLDD---- 1341

Query: 1337 MLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCM 1386
            +L +S  ++ +   +++E TL ++ +L++  +L  L+ IS+ P F   W+GVL R++T M
Sbjct: 1342 VLTFSIENSRKTLKKTVEETLVLATKLMSKAFLQSLQDISQQPSFCRLWVGVLNRLETYM 1401

BLAST of PI0027210 vs. ExPASy Swiss-Prot
Match: Q9R1D7 (Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Cricetulus griseus OX=10029 GN=GBF1 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.3e-90
Identity = 386/1732 (22.29%), Postives = 666/1732 (38.45%), Query Frame = 0

Query: 83   IDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLIVLGITN 142
            I+P++++ P L+VI+S+D     TG+AL+++ K +   + D    G  + +  +   +T+
Sbjct: 59   IEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTH 118

Query: 143  CKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLL 202
             +   TD  +++ V+MK+LQVL  L+       L ++SVC I+ +CF +  +     +LL
Sbjct: 119  ARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFE-MRLSELL 178

Query: 203  QRTARYTMNELIQIIFSRLPE--------------------------------------- 262
            +++A +T+ +++Q++F+RLP+                                       
Sbjct: 179  RKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPP 238

Query: 263  --------------------------------------------------------IEVR 322
                                                                    +E+ 
Sbjct: 239  RHMTRVTPGSELPAPNGATLSCNLTSGMPFIDVPSSISSASSEAASAVVSPCTDSGLELS 298

Query: 323  DGEESESDTEDADLGGS------LDSG--------------------------------- 382
                S+ D  D +  GS       +SG                                 
Sbjct: 299  SQTTSKEDLTDLEQAGSPRESTTTESGSNEIGVSDQLDPQEGSHVEKAQSASVESIPEVL 358

Query: 383  ---------------------------------------YGIRCVIDVFHFLCSLLNVVE 442
                                                   YG+ C+ ++F FL SL N  +
Sbjct: 359  EECTSPPDHSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHD 418

Query: 443  MLEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGA 502
                       R   E +    L L+  A+E +   + +   LL +++D++  HL    +
Sbjct: 419  -----------RHNSEGMIHMGLHLLTVALESA--PVAQCQTLLGLIKDEMCRHLFQLLS 478

Query: 503  ASNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFIYVALKLASFGNST---QIQEVALEGI 562
                 + +        ++  +R  ++ QLE  ++   +++ +  N     +++E+ALE I
Sbjct: 479  VERLNLYAASLRVCFLLFESMREHLKFQLE-MYMKKLMEIITVENPKMPYEMKEMALEAI 538

Query: 563  INFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLCKLSFPTGSPLTTLNIQAFEGLVIVI 622
            +   R  SF+ E Y+NYDCD    NLFE++ KLL K +FP    L T ++ + + L+ VI
Sbjct: 539  VQLWRIPSFVTELYINYDCDYYCANLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVI 598

Query: 623  -----HNIAEKLD----KHKEETCGGSGNLRVYPAQVDEYIPFWEEKSKED--------- 682
                 H  A+ L+    + K+ET   S     Y A          E++  D         
Sbjct: 599  DSTEAHCQAKVLNTLTQQEKKETSRPS-----YEAVDSTQEANSTERATIDGKATGMASD 658

Query: 683  -----LDLEDWL----------------------RYVR---------------VRKAQKK 742
                 L    WL                      ++ R               +    KK
Sbjct: 659  ALGLHLQSGGWLSAEHGKPRCNDVEEAGDSGADKKFTRKPPRFSCLLPDPRELIEIKNKK 718

Query: 743  KILIAG-HHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRYTHGLDKQFVGEYLGDPDQ 802
            K+LI G   FN+  KKG+ +L+   L++ P D    A + R    LDK+ +GE++   D+
Sbjct: 719  KLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVS--DR 778

Query: 803  FHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFSERFYELQSSNTF 862
             ++ +L  F  TF F G+ LD ALR YLE FRLPGEA  IHR+LEAF+E +     S  F
Sbjct: 779  KNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEAFTEHWRSCNGS-PF 838

Query: 863  ASKDTVFVLCYSLIMLNTDQHNPQVKKK---MTEDEFIRNNREINAGKDLPRDYLSELFH 922
            A+ D  F L Y++IMLNTDQHN  V+K+   MT +EF +N + +N GKD  +D L +++H
Sbjct: 839  ANSDACFALAYAVIMLNTDQHNHNVRKQNVPMTLEEFRKNLKGVNGGKDFEQDILEDMYH 898

Query: 923  SISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSCDFDPRLGRDMFGCIAGPSVA 982
            +I N  I++ P+    L      W  L++R    +             D+F    GP++A
Sbjct: 899  AIKNEEIVM-PEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIA 958

Query: 983  SLAAFFEHADEDEMLNECIEGLFSITKIT-QYGLEDTLDELLAMFCKFTTLLNPYASAEE 1042
            +L+  F+ + E+ ++ + I G      I+  YGL D  D L+   CKFT L +   S E 
Sbjct: 959  ALSYVFDKSIEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSS--ESIEN 1018

Query: 1043 TLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLLPQSVIDFEVAS 1102
               VF  + K  +A   VF +A+  GD +R GW+NI++ +L+L R +LLPQ++++ E   
Sbjct: 1019 LPTVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAVLQLFRAQLLPQAMVEVE--- 1078

Query: 1103 TSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLSLDSMEDS-----LTLNLNEYE 1162
                D    +  +    ++      +SS ++  F  +L+L   E S      T N     
Sbjct: 1079 ----DFVDPNGKISLQREEMPSNRGESSVLS--FVSWLTLSGPEQSSVRGPSTENQEAKR 1138

Query: 1163 QNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKFSTPVEEEETVGFCWDL 1222
              L  +KQC    + + S  +  E+L  L ++L+   A +         +EE   FC ++
Sbjct: 1139 VALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTADE------ETYDEEDAAFCLEM 1198

Query: 1223 IITMTMANIYRFQVFWPSFHEYL-QTVVQFPLFSAIPFAEKAVLGLFKVCLTLLSTYQPD 1282
            ++ + + N  R    W +  ++L    VQ   F  +   E+AV+GL ++ + LL   + +
Sbjct: 1199 LLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFL--VERAVVGLLRLAIRLL---RRE 1258

Query: 1283 KLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQSQIGWKSLLHLLSAT 1342
            ++  +++  S+ ++ ++   +L      +   + ++L    AN+ S   W +L  LL   
Sbjct: 1259 EISGQVLL-SLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECI 1318

Query: 1343 G--------------------------------RHPETYDQG------------------ 1385
            G                                ++  + D+G                  
Sbjct: 1319 GSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIH 1378

BLAST of PI0027210 vs. ExPASy Swiss-Prot
Match: Q92538 (Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Homo sapiens OX=9606 GN=GBF1 PE=1 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 4.1e-89
Identity = 309/1263 (24.47%), Postives = 532/1263 (42.12%), Query Frame = 0

Query: 83   IDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLIVLGITN 142
            I+P++++ P L+VI+S+D     TG+AL+++ K +   + D    G  + +  +   +T+
Sbjct: 59   IEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTH 118

Query: 143  CKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLL 202
             +   TD  +++ V+MK+LQVL  L+       L ++SVC I+ +CF +  +     +LL
Sbjct: 119  ARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFE-MRLSELL 178

Query: 203  QRTARYTMNELIQIIFSRLPEI-------------------------------------- 262
            +++A +T+ +++Q++F+RLP+                                       
Sbjct: 179  RKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPP 238

Query: 263  ----EVRDGEE----------------------------SESDTEDADLGGSLDSG---- 322
                +V  G E                            + S+   A +  S DSG    
Sbjct: 239  RHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFS 298

Query: 323  ------------------------------------------------------------ 382
                                                                        
Sbjct: 299  SQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVL 358

Query: 383  -----------------------------------------YGIRCVIDVFHFLCSLLNV 442
                                                     YG+ C+ ++F FL SL N 
Sbjct: 359  EECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNP 418

Query: 443  VEMLEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHY 502
             +           R   E +    L L+  A+E +   + +   LL +++D++  HL   
Sbjct: 419  HD-----------RHNSEVMIHMGLHLLTVALESA--PVAQCQTLLGLIKDEMCRHLFQL 478

Query: 503  GAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFIYVALKLASFGNST---QIQEVALE 562
             +     + +        ++  +R  ++ Q+E  +I   +++ +  N     +++E+ALE
Sbjct: 479  LSIERLNLYAASLRVCFLLFESMREHLKFQME-MYIKKLMEIITVENPKMPYEMKEMALE 538

Query: 563  GIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLCKLSFPTGSPLTTLNIQAFEGLVI 622
             I+   R  SF+ E Y+NYDCD    NLFEE+ KLL K +FP    L T ++ + + L+ 
Sbjct: 539  AIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLT 598

Query: 623  VI-----HNIAEKLD----KHKEETCGGS------------------------------- 682
            VI     H  A+ L+    + K+ET   S                               
Sbjct: 599  VIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPG 658

Query: 683  ----GNLRVYPAQVDEYIPFWEEKSKEDLD-------------LEDWLRYVRVRKAQKKK 742
                G  R+ P          EE      D             L D    + ++   KKK
Sbjct: 659  LHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIK--NKKK 718

Query: 743  ILIAG-HHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRYTHGLDKQFVGEYLGDPDQF 802
            +LI G   FN+  KKG+ +L+   L++ P D    A + R    LDK+ +GE++   D+ 
Sbjct: 719  LLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVS--DRK 778

Query: 803  HVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFSERFYELQSSNTFA 862
            ++ +L  F  TF F G+ LD ALR YLE FRLPGEA  I R+LEAF+ER+     S  FA
Sbjct: 779  NIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGS-PFA 838

Query: 863  SKDTVFVLCYSLIMLNTDQHNPQVKKK---MTEDEFIRNNREINAGKDLPRDYLSELFHS 922
            + D  F L Y++IMLNTDQHN  V+K+   MT +EF +N + +N GKD  +D L +++H+
Sbjct: 839  NSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHA 898

Query: 923  ISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSCDFDPRLGRDMFGCIAGPSVAS 982
            I N  I++ P+    L      W  L++R    +             D+F    GP++A+
Sbjct: 899  IKNEEIVM-PEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAA 958

Query: 983  LAAFFEHADEDEMLNECIEGLFSITKIT-QYGLEDTLDELLAMFCKFTTLLNPYASAEET 1042
            L+  F+ + E+ ++ + I G      I+  YGL D  D L+   CKFT L +   S E  
Sbjct: 959  LSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSS--ESIENL 1018

Query: 1043 LFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLLPQSVIDFEVAST 1097
              VF  + K  +A   VF +A+  GD +R GW+NI++ +L+L R +LLP+++I+ E    
Sbjct: 1019 PSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVE---- 1078

BLAST of PI0027210 vs. ExPASy TrEMBL
Match: A0A5A7V061 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00190 PE=4 SV=1)

HSP 1 Score: 2712.2 bits (7029), Expect = 0.0e+00
Identity = 1360/1387 (98.05%), Postives = 1376/1387 (99.21%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPS+LNSPYI+TID+TYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVE 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVEM+EVGDGGLGSRTADEDVQLFALVLINSAVE
Sbjct: 241  LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 300

Query: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360
            LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA
Sbjct: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360

Query: 361  FFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420
            F IYVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL
Sbjct: 361  FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420

Query: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480
            CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF
Sbjct: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480

Query: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKA 540
            WEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLSQLVSDPPDP+A
Sbjct: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPRA 540

Query: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600
            YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG
Sbjct: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600

Query: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660
            EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI
Sbjct: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660

Query: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720
            RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM
Sbjct: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720

Query: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLD 780
            SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTLD
Sbjct: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLD 780

Query: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840
            ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC
Sbjct: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840

Query: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLS 900
            LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGM SRFSQFLS
Sbjct: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLS 900

Query: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960
            LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF
Sbjct: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960

Query: 961  STPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020
            STPVEEEETVGFCWDLIITMTM N+YRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG
Sbjct: 961  STPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020

Query: 1021 LFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQ 1080
            LFKVCL LLST+QPDKLPEELIFKSINLMWMLDKEILD CFESITQSVSKIL+EYPANLQ
Sbjct: 1021 LFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANLQ 1080

Query: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140
            SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS
Sbjct: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140

Query: 1141 PLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLF 1200
            PLDKNLKILD+LSDSVNFLVQWYR+YCAESGNSFSV SNASSSSLDDKGLGSSNFAL LF
Sbjct: 1141 PLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALALF 1200

Query: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260
            LKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLHE
Sbjct: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260

Query: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320
            KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC
Sbjct: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320

Query: 1321 MKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380
            MKAD+GSYGESSLKELVPELLRKIITTMR KEILVKKEGEDLWEITYIQIQWIAPGIKDE
Sbjct: 1321 MKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380

Query: 1381 LFPEECF 1388
            LFPEECF
Sbjct: 1381 LFPEECF 1387

BLAST of PI0027210 vs. ExPASy TrEMBL
Match: A0A1S3C8R1 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo OX=3656 GN=LOC103497742 PE=4 SV=1)

HSP 1 Score: 2711.0 bits (7026), Expect = 0.0e+00
Identity = 1359/1387 (97.98%), Postives = 1376/1387 (99.21%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPS+LNSPYI+TID+TYDS
Sbjct: 3    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 62

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 63   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 122

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 123  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 182

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 183  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 242

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVE 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVEM+EVGDGGLGSRTADEDVQLFALVLINSAVE
Sbjct: 243  LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 302

Query: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360
            LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA
Sbjct: 303  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 362

Query: 361  FFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420
            F IYVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEE+GKLL
Sbjct: 363  FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEMGKLL 422

Query: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480
            CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF
Sbjct: 423  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 482

Query: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKA 540
            WEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLSQLVSDPPDP+A
Sbjct: 483  WEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPRA 542

Query: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600
            YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG
Sbjct: 543  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 602

Query: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660
            EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI
Sbjct: 603  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 662

Query: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720
            RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM
Sbjct: 663  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 722

Query: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLD 780
            SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTLD
Sbjct: 723  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLD 782

Query: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840
            ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC
Sbjct: 783  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 842

Query: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLS 900
            LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGM SRFSQFLS
Sbjct: 843  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLS 902

Query: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960
            LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF
Sbjct: 903  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 962

Query: 961  STPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020
            STPVEEEETVGFCWDLIITMTM N+YRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG
Sbjct: 963  STPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1022

Query: 1021 LFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQ 1080
            LFKVCL LLST+QPDKLPEELIFKSINLMWMLDKEILD CFESITQSVSKIL+EYPANLQ
Sbjct: 1023 LFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANLQ 1082

Query: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140
            SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS
Sbjct: 1083 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1142

Query: 1141 PLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLF 1200
            PLDKNLKILD+LSDSVNFLVQWYR+YCAESGNSFSV SNASSSSLDDKGLGSSNFAL LF
Sbjct: 1143 PLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALALF 1202

Query: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260
            LKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLHE
Sbjct: 1203 LKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1262

Query: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320
            KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC
Sbjct: 1263 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1322

Query: 1321 MKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380
            MKAD+GSYGESSLKELVPELLRKIITTMR KEILVKKEGEDLWEITYIQIQWIAPGIKDE
Sbjct: 1323 MKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1382

Query: 1381 LFPEECF 1388
            LFPEECF
Sbjct: 1383 LFPEECF 1389

BLAST of PI0027210 vs. ExPASy TrEMBL
Match: A0A0A0LF22 (SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G004740 PE=4 SV=1)

HSP 1 Score: 2704.1 bits (7008), Expect = 0.0e+00
Identity = 1356/1388 (97.69%), Postives = 1378/1388 (99.28%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVG+ILAVIRRPPS+LNSPYISTID+TYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPGAKDAINLIV+GITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVE-MLEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGSLDSGYGIRCVIDVFHFLCSLLNVVE M+EVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361  AFFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 420
            +FF+YVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EEIGKL
Sbjct: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGG GNLRVYPAQVDEYIP
Sbjct: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLS LVSDPPDPK
Sbjct: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDP QFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720

Query: 721  MSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTL 780
            MS DFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTL
Sbjct: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHD+KPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGM SRFSQFL
Sbjct: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900

Query: 901  SLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKF+KQCRIGNIFSNS+NILDEALLNLGRSLIFAAAGKGQK
Sbjct: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960

Query: 961  FSTPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTP+EEEETVGFCWDLIITMTMAN+YRFQVFWP+FHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020

Query: 1021 GLFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANL 1080
            GLFKVCL LLSTYQPDK+PEELIFKSINLMWMLDKEILDTCFESITQSVSKIL+EYPANL
Sbjct: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140

Query: 1141 SPLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTL 1200
            SPLDKNLKILD+LSDSVNFLVQWYR+YCAESGNSFSVASNASSSSLD+KGLGSSNFALTL
Sbjct: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260

Query: 1261 EKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSM+GTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320

Query: 1321 CMKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKADLGSYGESSLK+L+PELLRKIITTMR KEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1381 ELFPEECF 1388

BLAST of PI0027210 vs. ExPASy TrEMBL
Match: A0A6J1FBD6 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucurbita moschata OX=3662 GN=LOC111443895 PE=4 SV=1)

HSP 1 Score: 2493.0 bits (6460), Expect = 0.0e+00
Identity = 1255/1387 (90.48%), Postives = 1326/1387 (95.60%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            M+KRSS  +D K+S KSKRRELGLSCMLNTEVGA+LAVIRRPPS+LN+PYI T DDTYDS
Sbjct: 1    MEKRSSRHDDGKESSKSKRRELGLSCMLNTEVGAVLAVIRRPPSELNAPYICTADDTYDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            SIQQSLKSLRALIF PQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   SIQQSLKSLRALIFQPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IF+EKTPGAKDAINLIVLGITNCKLEKTD+VTEDAVMMK+LQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFEEKTPGAKDAINLIVLGITNCKLEKTDVVTEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+E RD E+SESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEFRDEEDSESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVE 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVE  E+ D GLGSRTADEDVQLFALVLINSAV 
Sbjct: 241  LGGSMDSGYGIRCVIDVFHFLCSLLNVVETAEMVD-GLGSRTADEDVQLFALVLINSAVG 300

Query: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360
            LSGDAIGKHPKLLRM+QDDLFHHLIHYGA SNPLVLSMICSTVLNIYHFLRRFVRLQLEA
Sbjct: 301  LSGDAIGKHPKLLRMIQDDLFHHLIHYGARSNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360

Query: 361  FFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420
            FFIYV L+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL
Sbjct: 361  FFIYVVLRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420

Query: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480
            CKLSFP GSPLTTL IQAFEGLVI+IHNIAEKL+  KEE+ GGS   R+YPAQV+ Y PF
Sbjct: 421  CKLSFPMGSPLTTLQIQAFEGLVIMIHNIAEKLE--KEESSGGSS--RIYPAQVNVYRPF 480

Query: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKA 540
            WEEKSK+DL  E+WL YVRVRKAQKKK+LIAGHHFNRDEKKGLAYLKL QLVSDPPDPKA
Sbjct: 481  WEEKSKDDLQYENWLDYVRVRKAQKKKVLIAGHHFNRDEKKGLAYLKLCQLVSDPPDPKA 540

Query: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600
            YA+FFRYT+GLDKQ +GEYLGDPDQFHV+VLAEFT+TFEFTGMILDTALRTYLETFRLPG
Sbjct: 541  YAFFFRYTNGLDKQLIGEYLGDPDQFHVRVLAEFTDTFEFTGMILDTALRTYLETFRLPG 600

Query: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660
            EAQKIHRILEAFSERFY LQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI
Sbjct: 601  EAQKIHRILEAFSERFYVLQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660

Query: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720
            RNNREINAGKDLPRDYLSELFHSISN+AIILSPQSGLQ DMNPSKWVELMNRSKIIQPFM
Sbjct: 661  RNNREINAGKDLPRDYLSELFHSISNHAIILSPQSGLQFDMNPSKWVELMNRSKIIQPFM 720

Query: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLD 780
             CDFDPRLGRDMF C+AGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTLD
Sbjct: 721  LCDFDPRLGRDMFACVAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLD 780

Query: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840
            ELLA+F K+TTLLNPYAS EETLF FSHDLKPKLATLAVFTIANNFGD+IRGGWRNIVDC
Sbjct: 781  ELLAIFSKYTTLLNPYASTEETLFAFSHDLKPKLATLAVFTIANNFGDSIRGGWRNIVDC 840

Query: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLS 900
            LLKLKRLKLLPQSVIDFEVAST+SND+A+SDSGVIFPSQDPKF TQQSSGMA RFSQFLS
Sbjct: 841  LLKLKRLKLLPQSVIDFEVASTTSNDMAKSDSGVIFPSQDPKFGTQQSSGMAGRFSQFLS 900

Query: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960
            LDSMEDSL+LNLNE+EQNLKF+KQCRIG+IFS+S+++ DEALLNLGRSLIFAAAGKGQKF
Sbjct: 901  LDSMEDSLSLNLNEFEQNLKFIKQCRIGSIFSSSSSLHDEALLNLGRSLIFAAAGKGQKF 960

Query: 961  STPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020
            STPVEEEETVGFCWDLI TM++AN+YRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG
Sbjct: 961  STPVEEEETVGFCWDLITTMSLANVYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020

Query: 1021 LFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQ 1080
            LFKVCL LLSTYQP+K PEELIFKSINLMWMLDKEILDTCFESITQSVSKI++EYPANLQ
Sbjct: 1021 LFKVCLKLLSTYQPEKHPEELIFKSINLMWMLDKEILDTCFESITQSVSKIIIEYPANLQ 1080

Query: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140
            + IGWKSLLHLLSATGRHPETY+QGVETLIMLMSD +HITRTNYTFCI+CAFSYVALKNS
Sbjct: 1081 TAIGWKSLLHLLSATGRHPETYEQGVETLIMLMSDGTHITRTNYTFCIECAFSYVALKNS 1140

Query: 1141 PLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLF 1200
            PLDKNLKILD LSDSVNFLVQWYR+YCAESGNS+SVASNASSSS +DKGLG SNFALTLF
Sbjct: 1141 PLDKNLKILDLLSDSVNFLVQWYRNYCAESGNSYSVASNASSSS-EDKGLG-SNFALTLF 1200

Query: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260
            +KLGEALRKTSLARREEIRNHA+ SLKKSF+LAEELDF  TNCI  FN ++FAMVDDLHE
Sbjct: 1201 IKLGEALRKTSLARREEIRNHAVISLKKSFMLAEELDFTSTNCINFFNLVVFAMVDDLHE 1260

Query: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320
            KMLEYSRRDNA+REARSMEGTLKISM+LLTDVYL++LKQISES GFRTFWLG+LRRMDTC
Sbjct: 1261 KMLEYSRRDNAKREARSMEGTLKISMDLLTDVYLLFLKQISESSGFRTFWLGILRRMDTC 1320

Query: 1321 MKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380
            MKADLGSYGESSLK+LVPELLRKIIT MR KEIL+KKEG+DLWEITYIQIQWIAP IK+E
Sbjct: 1321 MKADLGSYGESSLKDLVPELLRKIITKMREKEILMKKEGDDLWEITYIQIQWIAPAIKEE 1380

Query: 1381 LFPEECF 1388
            LFPEE F
Sbjct: 1381 LFPEESF 1380

BLAST of PI0027210 vs. ExPASy TrEMBL
Match: A0A6J1DTF7 (ARF guanine-nucleotide exchange factor GNL2 OS=Momordica charantia OX=3673 GN=LOC111024228 PE=4 SV=1)

HSP 1 Score: 2492.2 bits (6458), Expect = 0.0e+00
Identity = 1247/1387 (89.91%), Postives = 1325/1387 (95.53%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            M KR SD+ED++DS KSKRRELGLSCMLNTEVGAILAVIRRPPS+LNSPYIS IDDT+DS
Sbjct: 1    MTKR-SDEEDDRDSSKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYISPIDDTFDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDV+QSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVVQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IF+EKTPGAKDAINLIVLGITNCKLEKTDL+TEDAVMMK+LQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFNEKTPGAKDAINLIVLGITNCKLEKTDLITEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSA+RGDLLQRTARYTM+ELIQIIFSRLPEIEVRDGE+SESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSANRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVE 300
            LGGSLDSGYGIRCVIDVFHFLCSL+NVVEM E+ D GL SRTADEDVQLFALVLINSAVE
Sbjct: 241  LGGSLDSGYGIRCVIDVFHFLCSLMNVVEMGEMMD-GLASRTADEDVQLFALVLINSAVE 300

Query: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360
            LSGDAIGKHP+LLRMVQDDLFHHLIHYGA  NPLVLSMICSTVLNIYHFLRRFVRLQLEA
Sbjct: 301  LSGDAIGKHPRLLRMVQDDLFHHLIHYGARCNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360

Query: 361  FFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420
            FF+YVAL+ AS+GNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNLFE+IGKLL
Sbjct: 361  FFVYVALRSASYGNSTQIQEVALEGIINFCRQSSFILETYVNYDCDPLRWNLFEDIGKLL 420

Query: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480
            CKLSFP GSPL++L IQAFEGLVI+IHNIAEKLDK +     GS +LRVYPA V EY PF
Sbjct: 421  CKLSFPAGSPLSSLQIQAFEGLVIMIHNIAEKLDKLEGGPSRGS-SLRVYPAHVSEYRPF 480

Query: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKA 540
            WEE+SKEDL+LE WL YVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLS LV DPPDPKA
Sbjct: 481  WEERSKEDLELEGWLNYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVPDPPDPKA 540

Query: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600
            YA+FFRYT+GLDKQFVGEYLGDPD FHV+VLAEFT TFEFTGMILDTALRTYLETFRLPG
Sbjct: 541  YAFFFRYTYGLDKQFVGEYLGDPDPFHVRVLAEFTGTFEFTGMILDTALRTYLETFRLPG 600

Query: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660
            EAQKIHRILEAFSERFY+LQSSNTF +KD VFVLCYSLIMLNTDQHNPQVK+KMTEDEFI
Sbjct: 601  EAQKIHRILEAFSERFYDLQSSNTFVNKDAVFVLCYSLIMLNTDQHNPQVKRKMTEDEFI 660

Query: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720
            RNNREIN GKDLPRDYLSELF SI+NNAIIL+PQSGLQLDMNPSKW+EL NRSKIIQPF+
Sbjct: 661  RNNREINGGKDLPRDYLSELFQSIANNAIILAPQSGLQLDMNPSKWIELTNRSKIIQPFV 720

Query: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLD 780
             CDFD RLGRDMF CIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQ GLEDTLD
Sbjct: 721  LCDFDIRLGRDMFACIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQCGLEDTLD 780

Query: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840
            ELLA+F KFTTLLNPYASAEETLF FSHDLKPKLATLAVFTIANNFGD+IRGGWRNIVDC
Sbjct: 781  ELLALFSKFTTLLNPYASAEETLFAFSHDLKPKLATLAVFTIANNFGDSIRGGWRNIVDC 840

Query: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLS 900
            LLKLKRLKLLPQSVI+FE+AST+ +D+A+S+SGVIFPSQDPKFCTQQSSGMASRFSQFLS
Sbjct: 841  LLKLKRLKLLPQSVIEFELASTAPHDLAKSESGVIFPSQDPKFCTQQSSGMASRFSQFLS 900

Query: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960
            LDSMEDSL+LNLNE+EQNLKF+KQCRIGNIFSNS+N+LD+ALLNLGRSLIFAAAGKGQKF
Sbjct: 901  LDSMEDSLSLNLNEFEQNLKFIKQCRIGNIFSNSSNLLDDALLNLGRSLIFAAAGKGQKF 960

Query: 961  STPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020
            STP+EEEETVGFCWDLIITM++AN++RFQVFWPSFHEYLQ V+QFPLFS IPFAEKAVLG
Sbjct: 961  STPIEEEETVGFCWDLIITMSLANVHRFQVFWPSFHEYLQAVIQFPLFSTIPFAEKAVLG 1020

Query: 1021 LFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQ 1080
            LFKVCL LLSTYQP+K PEELIFKSINLMWMLDKEILDTCFE+ITQSVSKIL+EYPANLQ
Sbjct: 1021 LFKVCLKLLSTYQPEKYPEELIFKSINLMWMLDKEILDTCFEAITQSVSKILIEYPANLQ 1080

Query: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140
            S IGWKSLLHLLSATGRHPETYDQGVETLIMLMSD +HITRTNYTFCIDCAFSYVALKNS
Sbjct: 1081 STIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDGTHITRTNYTFCIDCAFSYVALKNS 1140

Query: 1141 PLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLF 1200
            PL+KNLKILD LSDSVNFL+QWYR+YCAESGNSFSV+SNASSSSL+DKGLGSSNFALTLF
Sbjct: 1141 PLEKNLKILDLLSDSVNFLIQWYRNYCAESGNSFSVSSNASSSSLEDKGLGSSNFALTLF 1200

Query: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260
            +KLGEALRKTSLARREEIRNHAI SLKKSF LAEELDF PTNCI CFN +IFAMVDDLHE
Sbjct: 1201 VKLGEALRKTSLARREEIRNHAIVSLKKSFFLAEELDFSPTNCINCFNLVIFAMVDDLHE 1260

Query: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320
            KMLEYSRRDNAEREARSMEGTLKISM+LLTDVYL++LK ISES GFRTFWLGVLRRMDTC
Sbjct: 1261 KMLEYSRRDNAEREARSMEGTLKISMDLLTDVYLLFLKPISESAGFRTFWLGVLRRMDTC 1320

Query: 1321 MKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380
            MKADLG+YGESSLK+LVP+LLRK+IT MR KEILV+KEG+DLWE TYIQIQWIAP IK+E
Sbjct: 1321 MKADLGAYGESSLKDLVPDLLRKLITNMREKEILVQKEGDDLWETTYIQIQWIAPSIKEE 1380

Query: 1381 LFPEECF 1388
            LFPEE F
Sbjct: 1381 LFPEESF 1384

BLAST of PI0027210 vs. NCBI nr
Match: KAA0061562.1 (ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa] >TYK10710.1 ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa])

HSP 1 Score: 2712.2 bits (7029), Expect = 0.0e+00
Identity = 1360/1387 (98.05%), Postives = 1376/1387 (99.21%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPS+LNSPYI+TID+TYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVE 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVEM+EVGDGGLGSRTADEDVQLFALVLINSAVE
Sbjct: 241  LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 300

Query: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360
            LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA
Sbjct: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360

Query: 361  FFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420
            F IYVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL
Sbjct: 361  FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420

Query: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480
            CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF
Sbjct: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480

Query: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKA 540
            WEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLSQLVSDPPDP+A
Sbjct: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPRA 540

Query: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600
            YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG
Sbjct: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600

Query: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660
            EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI
Sbjct: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660

Query: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720
            RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM
Sbjct: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720

Query: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLD 780
            SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTLD
Sbjct: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLD 780

Query: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840
            ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC
Sbjct: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840

Query: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLS 900
            LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGM SRFSQFLS
Sbjct: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLS 900

Query: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960
            LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF
Sbjct: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960

Query: 961  STPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020
            STPVEEEETVGFCWDLIITMTM N+YRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG
Sbjct: 961  STPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020

Query: 1021 LFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQ 1080
            LFKVCL LLST+QPDKLPEELIFKSINLMWMLDKEILD CFESITQSVSKIL+EYPANLQ
Sbjct: 1021 LFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANLQ 1080

Query: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140
            SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS
Sbjct: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140

Query: 1141 PLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLF 1200
            PLDKNLKILD+LSDSVNFLVQWYR+YCAESGNSFSV SNASSSSLDDKGLGSSNFAL LF
Sbjct: 1141 PLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALALF 1200

Query: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260
            LKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLHE
Sbjct: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260

Query: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320
            KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC
Sbjct: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320

Query: 1321 MKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380
            MKAD+GSYGESSLKELVPELLRKIITTMR KEILVKKEGEDLWEITYIQIQWIAPGIKDE
Sbjct: 1321 MKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380

Query: 1381 LFPEECF 1388
            LFPEECF
Sbjct: 1381 LFPEECF 1387

BLAST of PI0027210 vs. NCBI nr
Match: XP_008458277.1 (PREDICTED: ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo])

HSP 1 Score: 2711.0 bits (7026), Expect = 0.0e+00
Identity = 1359/1387 (97.98%), Postives = 1376/1387 (99.21%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPS+LNSPYI+TID+TYDS
Sbjct: 3    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 62

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 63   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 122

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 123  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 182

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 183  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 242

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVE 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVEM+EVGDGGLGSRTADEDVQLFALVLINSAVE
Sbjct: 243  LGGSMDSGYGIRCVIDVFHFLCSLLNVVEMVEVGDGGLGSRTADEDVQLFALVLINSAVE 302

Query: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360
            LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA
Sbjct: 303  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 362

Query: 361  FFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420
            F IYVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEE+GKLL
Sbjct: 363  FSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEMGKLL 422

Query: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480
            CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF
Sbjct: 423  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 482

Query: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKA 540
            WEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLSQLVSDPPDP+A
Sbjct: 483  WEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPRA 542

Query: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600
            YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG
Sbjct: 543  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 602

Query: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660
            EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI
Sbjct: 603  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 662

Query: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720
            RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM
Sbjct: 663  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 722

Query: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLD 780
            SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTLD
Sbjct: 723  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLD 782

Query: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840
            ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC
Sbjct: 783  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 842

Query: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLS 900
            LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGM SRFSQFLS
Sbjct: 843  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLS 902

Query: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960
            LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF
Sbjct: 903  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 962

Query: 961  STPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020
            STPVEEEETVGFCWDLIITMTM N+YRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG
Sbjct: 963  STPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1022

Query: 1021 LFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQ 1080
            LFKVCL LLST+QPDKLPEELIFKSINLMWMLDKEILD CFESITQSVSKIL+EYPANLQ
Sbjct: 1023 LFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANLQ 1082

Query: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140
            SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS
Sbjct: 1083 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1142

Query: 1141 PLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLF 1200
            PLDKNLKILD+LSDSVNFLVQWYR+YCAESGNSFSV SNASSSSLDDKGLGSSNFAL LF
Sbjct: 1143 PLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALALF 1202

Query: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260
            LKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLHE
Sbjct: 1203 LKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1262

Query: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320
            KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC
Sbjct: 1263 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1322

Query: 1321 MKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380
            MKAD+GSYGESSLKELVPELLRKIITTMR KEILVKKEGEDLWEITYIQIQWIAPGIKDE
Sbjct: 1323 MKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1382

Query: 1381 LFPEECF 1388
            LFPEECF
Sbjct: 1383 LFPEECF 1389

BLAST of PI0027210 vs. NCBI nr
Match: XP_004139429.1 (ARF guanine-nucleotide exchange factor GNL2 [Cucumis sativus] >KGN60625.1 hypothetical protein Csa_019390 [Cucumis sativus])

HSP 1 Score: 2704.1 bits (7008), Expect = 0.0e+00
Identity = 1356/1388 (97.69%), Postives = 1378/1388 (99.28%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVG+ILAVIRRPPS+LNSPYISTID+TYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPGAKDAINLIV+GITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVE-MLEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGSLDSGYGIRCVIDVFHFLCSLLNVVE M+EVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361  AFFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 420
            +FF+YVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EEIGKL
Sbjct: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGG GNLRVYPAQVDEYIP
Sbjct: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLS LVSDPPDPK
Sbjct: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDP QFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720

Query: 721  MSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTL 780
            MS DFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTL
Sbjct: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHD+KPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGM SRFSQFL
Sbjct: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900

Query: 901  SLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKF+KQCRIGNIFSNS+NILDEALLNLGRSLIFAAAGKGQK
Sbjct: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960

Query: 961  FSTPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTP+EEEETVGFCWDLIITMTMAN+YRFQVFWP+FHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020

Query: 1021 GLFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANL 1080
            GLFKVCL LLSTYQPDK+PEELIFKSINLMWMLDKEILDTCFESITQSVSKIL+EYPANL
Sbjct: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140

Query: 1141 SPLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTL 1200
            SPLDKNLKILD+LSDSVNFLVQWYR+YCAESGNSFSVASNASSSSLD+KGLGSSNFALTL
Sbjct: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260

Query: 1261 EKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSM+GTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320

Query: 1321 CMKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKADLGSYGESSLK+L+PELLRKIITTMR KEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1381 ELFPEECF 1388

BLAST of PI0027210 vs. NCBI nr
Match: XP_038889761.1 (ARF guanine-nucleotide exchange factor GNL2 [Benincasa hispida])

HSP 1 Score: 2645.2 bits (6855), Expect = 0.0e+00
Identity = 1323/1389 (95.25%), Postives = 1365/1389 (98.27%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            M+KRSSDDE+EKDSPKSKRRELGLSCMLNTEVGA+LAVIRRPPSDLNSPYISTIDDTYDS
Sbjct: 1    MEKRSSDDEEEKDSPKSKRRELGLSCMLNTEVGAVLAVIRRPPSDLNSPYISTIDDTYDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IF+EKTPG KDAINLIVLGITNCKLEKTDLVTEDAVMMK+LQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFNEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGE+SESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEDSESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGL----GSRTADEDVQLFALVLIN 300
            LGGSLDSGYGIRCVIDVFHFLCSLLNVVE++E+GDGGL    GSRTADEDVQLFALVLIN
Sbjct: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIVEIGDGGLGMGGGSRTADEDVQLFALVLIN 300

Query: 301  SAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRL 360
            SAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGA+SNPLVLSMICSTVLNIYHFLRRFVRL
Sbjct: 301  SAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGASSNPLVLSMICSTVLNIYHFLRRFVRL 360

Query: 361  QLEAFFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEI 420
            QLEAFF+YVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEI
Sbjct: 361  QLEAFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEI 420

Query: 421  GKLLCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDE 480
            GKLLCKLSFPTGSPLTTLNIQAFEGLVI+IHNIAEKLDKHKEE+CGG+G+LRVYPAQV E
Sbjct: 421  GKLLCKLSFPTGSPLTTLNIQAFEGLVIMIHNIAEKLDKHKEESCGGNGSLRVYPAQVTE 480

Query: 481  YIPFWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPP 540
            Y PFWEEKSKEDL+LEDWL YVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPP
Sbjct: 481  YRPFWEEKSKEDLELEDWLNYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPP 540

Query: 541  DPKAYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETF 600
            DPKAYA+FFRYTHGLDKQF+GEYLGDPDQFHVKVLAEFT+TFEFTGMILDTALRTYLETF
Sbjct: 541  DPKAYAFFFRYTHGLDKQFIGEYLGDPDQFHVKVLAEFTDTFEFTGMILDTALRTYLETF 600

Query: 601  RLPGEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTE 660
            RLPGEAQKIHRILEAFSERFY+LQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTE
Sbjct: 601  RLPGEAQKIHRILEAFSERFYDLQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTE 660

Query: 661  DEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKII 720
            DEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKII
Sbjct: 661  DEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKII 720

Query: 721  QPFMSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLE 780
            QPFM CDFDPRLGRDMF CIAGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLE
Sbjct: 721  QPFMLCDFDPRLGRDMFACIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLE 780

Query: 781  DTLDELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRN 840
            DTLDELLA+F KFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGD+IRGGW+N
Sbjct: 781  DTLDELLALFSKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDSIRGGWKN 840

Query: 841  IVDCLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFS 900
            IVDCLLKLKRLKLLP SVIDFEVASTSSNDVARS+SGVIFPSQDPKFC+QQSSGMASRFS
Sbjct: 841  IVDCLLKLKRLKLLPPSVIDFEVASTSSNDVARSESGVIFPSQDPKFCSQQSSGMASRFS 900

Query: 901  QFLSLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGK 960
            QFLSLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFS+S+NI DEAL +LGRSLIFAAAGK
Sbjct: 901  QFLSLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSSSSNIHDEALFHLGRSLIFAAAGK 960

Query: 961  GQKFSTPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEK 1020
            GQKFSTPVEEEETVGFCWDLIITMTMAN+YRFQVFWPSFHEYLQ VVQFPLFSAIPFAEK
Sbjct: 961  GQKFSTPVEEEETVGFCWDLIITMTMANLYRFQVFWPSFHEYLQAVVQFPLFSAIPFAEK 1020

Query: 1021 AVLGLFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYP 1080
            AVLGLFKVCL LLSTYQP+KLPEELIFKSINLMWMLDKEILDTCFESIT+SVSKI++EYP
Sbjct: 1021 AVLGLFKVCLRLLSTYQPEKLPEELIFKSINLMWMLDKEILDTCFESITKSVSKIIIEYP 1080

Query: 1081 ANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVA 1140
            ANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSD +HITRTNYTFCIDCAFSYVA
Sbjct: 1081 ANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDGTHITRTNYTFCIDCAFSYVA 1140

Query: 1141 LKNSPLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFA 1200
            LKNSPL+KNLKILDSLSDSVNFLVQWYR+YCAESGNSFSVASNASSSSLDDK LGSSNF+
Sbjct: 1141 LKNSPLEKNLKILDSLSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDDKSLGSSNFS 1200

Query: 1201 LTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVD 1260
            LTLFLKLGEALRKTSLARREEIRNHAI SLKKSFVLAEELDFPPTNCI CFNNIIFAMVD
Sbjct: 1201 LTLFLKLGEALRKTSLARREEIRNHAIASLKKSFVLAEELDFPPTNCISCFNNIIFAMVD 1260

Query: 1261 DLHEKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRR 1320
            DLHEKMLEYSRRDNAEREARSMEGTLKISME+LTD+YL+YLKQISES GFRTFWLGVLRR
Sbjct: 1261 DLHEKMLEYSRRDNAEREARSMEGTLKISMEVLTDIYLLYLKQISESAGFRTFWLGVLRR 1320

Query: 1321 MDTCMKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPG 1380
            MDTCMKADLGSYGESSLKELVP+LLRKIIT MR KEIL+KKEGEDLWEITYIQIQWIAPG
Sbjct: 1321 MDTCMKADLGSYGESSLKELVPDLLRKIITNMREKEILIKKEGEDLWEITYIQIQWIAPG 1380

Query: 1381 IKDELFPEE 1386
            IKDELFPEE
Sbjct: 1381 IKDELFPEE 1389

BLAST of PI0027210 vs. NCBI nr
Match: KAG6586424.1 (ARF guanine-nucleotide exchange factor GNL2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021279.1 ARF guanine-nucleotide exchange factor GNL2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2494.5 bits (6464), Expect = 0.0e+00
Identity = 1255/1387 (90.48%), Postives = 1326/1387 (95.60%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDS 60
            M+KRSS  +D K+S KSKRRELGLSCMLNTEVGA+LAVIRRPPS+LN+PYI T DDTYDS
Sbjct: 1    MEKRSSRHDDGKESSKSKRRELGLSCMLNTEVGAVLAVIRRPPSELNAPYICTADDTYDS 60

Query: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            SIQQSLKSLRALIF PQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   SIQQSLKSLRALIFQPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IF+EKTPGAKDAINLIVLGITNCKLEKTD+VTEDAVMMK+LQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFEEKTPGAKDAINLIVLGITNCKLEKTDVVTEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+E RDGE+SESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEFRDGEDSESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVE 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVE  E+ D GLGSRTADEDVQLFALVLINSAV 
Sbjct: 241  LGGSMDSGYGIRCVIDVFHFLCSLLNVVETAEMVD-GLGSRTADEDVQLFALVLINSAVG 300

Query: 301  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360
            LSGDAIGKHPKLLRM+QDDLFHHLIHYGA SNPLVLSMICSTVLNIYHFLRRFVRLQLEA
Sbjct: 301  LSGDAIGKHPKLLRMIQDDLFHHLIHYGARSNPLVLSMICSTVLNIYHFLRRFVRLQLEA 360

Query: 361  FFIYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420
            FFIYV L+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL
Sbjct: 361  FFIYVVLRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLL 420

Query: 421  CKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPF 480
            CKLSFP GSPLTTL IQAFEGLVI+IHNIAEKL+  KEE+ GGS   R+YPAQV+ Y PF
Sbjct: 421  CKLSFPMGSPLTTLQIQAFEGLVIMIHNIAEKLE--KEESSGGSS--RIYPAQVNVYRPF 480

Query: 481  WEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKA 540
            WEEKSK+DL  E+WL YVRVRKAQKKK+LIAGHHFNRDEKKGLAYLKL QLVSDPPDPKA
Sbjct: 481  WEEKSKDDLQYENWLDYVRVRKAQKKKVLIAGHHFNRDEKKGLAYLKLCQLVSDPPDPKA 540

Query: 541  YAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPG 600
            YA+FFRYT+GLDKQ +GEYLGDPDQFHV+VLAEFT+TFEFTGMILDTALRTYLETFRLPG
Sbjct: 541  YAFFFRYTNGLDKQLIGEYLGDPDQFHVRVLAEFTDTFEFTGMILDTALRTYLETFRLPG 600

Query: 601  EAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660
            EAQKIHRILEAFSERFY LQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI
Sbjct: 601  EAQKIHRILEAFSERFYVLQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFI 660

Query: 661  RNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFM 720
            RNNREINAGKDLPRDYLSELFHSISN+AIILSPQSGLQ DMNPSKWVELMNRSKIIQPFM
Sbjct: 661  RNNREINAGKDLPRDYLSELFHSISNHAIILSPQSGLQFDMNPSKWVELMNRSKIIQPFM 720

Query: 721  SCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLD 780
             CDFDPRLGRDMF C+AGPSVASLAAFFEHADEDEMLNECIEGLFSI KITQYGLEDTLD
Sbjct: 721  LCDFDPRLGRDMFACVAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLD 780

Query: 781  ELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDC 840
            ELLA+F K+TTLLNPYAS EETLF FSHDLKPKLATLAVFTIANNFGD+IRGGWRNIVDC
Sbjct: 781  ELLAIFSKYTTLLNPYASTEETLFAFSHDLKPKLATLAVFTIANNFGDSIRGGWRNIVDC 840

Query: 841  LLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLS 900
            LLKLKRLKLLPQSVIDFEVAST+SND+A+SDSGVIFPSQDPKF TQQSSGMA RFSQFLS
Sbjct: 841  LLKLKRLKLLPQSVIDFEVASTTSNDMAKSDSGVIFPSQDPKFGTQQSSGMAGRFSQFLS 900

Query: 901  LDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKF 960
            LDSMEDSL+LNLNE+EQNLKF+KQCRIG+IFS+S+++ DEALLNLGRSLIFAAAGKGQKF
Sbjct: 901  LDSMEDSLSLNLNEFEQNLKFIKQCRIGSIFSSSSSLHDEALLNLGRSLIFAAAGKGQKF 960

Query: 961  STPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020
            STPVEEEETVGFCWDLI TM++AN+YRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG
Sbjct: 961  STPVEEEETVGFCWDLITTMSLANVYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLG 1020

Query: 1021 LFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQ 1080
            LFKVCL LLSTYQP+K PEELIFKSINLMWMLDKEILDTCFESITQSVSKI++EYPANLQ
Sbjct: 1021 LFKVCLKLLSTYQPEKHPEELIFKSINLMWMLDKEILDTCFESITQSVSKIIIEYPANLQ 1080

Query: 1081 SQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNS 1140
            + IGWKSLLHLLSATGRHPETY+QGVETLIMLMSD +HITRTNYTFCI+CAFSYVALKNS
Sbjct: 1081 TAIGWKSLLHLLSATGRHPETYEQGVETLIMLMSDGTHITRTNYTFCIECAFSYVALKNS 1140

Query: 1141 PLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLF 1200
            PLDKNLKILD LSDSVNFLVQWYR+YCAESGNS+SVASNASSSS +DKG G SNFALTLF
Sbjct: 1141 PLDKNLKILDLLSDSVNFLVQWYRNYCAESGNSYSVASNASSSS-EDKGFG-SNFALTLF 1200

Query: 1201 LKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHE 1260
            +KLGEALRKTSLARREEIRNHA+ SLKKSF+LAEELDF  TNCI  FN ++FAMVDDLHE
Sbjct: 1201 IKLGEALRKTSLARREEIRNHAVISLKKSFMLAEELDFTSTNCINFFNLVVFAMVDDLHE 1260

Query: 1261 KMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTC 1320
            KMLEYSRRDNA+REARSMEGTLKISM+LLTDVYL++LKQISES GFRTFWLG+LRRMDTC
Sbjct: 1261 KMLEYSRRDNAKREARSMEGTLKISMDLLTDVYLLFLKQISESSGFRTFWLGILRRMDTC 1320

Query: 1321 MKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDE 1380
            MKADLGSYGESSLK+LVPELLRKIIT MR KEIL+KKEG+DLWEITYIQIQWIAP IK+E
Sbjct: 1321 MKADLGSYGESSLKDLVPELLRKIITKMREKEILMKKEGDDLWEITYIQIQWIAPAIKEE 1380

Query: 1381 LFPEECF 1388
            LFPEE F
Sbjct: 1381 LFPEESF 1380

BLAST of PI0027210 vs. TAIR 10
Match: AT5G19610.1 (GNOM-like 2 )

HSP 1 Score: 1852.0 bits (4796), Expect = 0.0e+00
Identity = 913/1381 (66.11%), Postives = 1140/1381 (82.55%), Query Frame = 0

Query: 16   KSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTID-DTYDSSIQQSLKSLRALIF 75
            ++KR+ELG+SCMLNTEVGA+LAVIRRP   L+  Y+S  + D  DSS+QQSLKSLRALIF
Sbjct: 7    RAKRKELGISCMLNTEVGAVLAVIRRP---LSESYLSPQETDHCDSSVQQSLKSLRALIF 66

Query: 76   HPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAIN 135
            +PQQ WRTIDPS+Y+SP L+VIQSD+IPA+AT VALS++LKI+K+EIFDEKTPGAKDA+N
Sbjct: 67   NPQQDWRTIDPSVYLSPFLEVIQSDEIPASATAVALSSILKILKIEIFDEKTPGAKDAMN 126

Query: 136  LIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQ 195
             IV GIT+C+LEKTDLV+EDAVMM++LQVL G+M H +S LL DQ+VCTIVNTCF VVQQ
Sbjct: 127  SIVSGITSCRLEKTDLVSEDAVMMRILQVLTGIMKHPSSELLEDQAVCTIVNTCFQVVQQ 186

Query: 196  SASRGDLLQRTARYTMNELIQIIFSRLPEIEVR---DGEESESDTEDADLGGSLDSGYGI 255
            S  RGDLLQR  RYTM+ELIQIIFSRLP+ EVR    GE+SESDT++ D+ G    GYGI
Sbjct: 187  STGRGDLLQRNGRYTMHELIQIIFSRLPDFEVRGDEGGEDSESDTDEIDMSG----GYGI 246

Query: 256  RCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPK 315
            RC ID+FHFLCSLLNVVE++E  + G    TADEDVQ+FALVLINSA+ELSGDAIG+HPK
Sbjct: 247  RCCIDIFHFLCSLLNVVEVVENLE-GTNVHTADEDVQIFALVLINSAIELSGDAIGQHPK 306

Query: 316  LLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFIYVALKLAS 375
            LLRMVQDDLFHHLIHYGA+S+PLVLSMICS +LNIYHFLR+F+RLQLEAFF +V L++ +
Sbjct: 307  LLRMVQDDLFHHLIHYGASSSPLVLSMICSCILNIYHFLRKFMRLQLEAFFSFVLLRVTA 366

Query: 376  FGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLCKLSFPTGSPL 435
            F     +QEVALEG+INFCRQ +FI+E YVNYDCDP+  N+FEE GK+LC+ +FPT  PL
Sbjct: 367  FTGFLPLQEVALEGLINFCRQPAFIVEAYVNYDCDPMCRNIFEETGKVLCRHTFPTSGPL 426

Query: 436  TTLNIQAFEGLVIVIHNIAEKLDKHK----EETCGGSGNLRVYPAQVDEYIPFWEEKSKE 495
            T++ IQAFEGLVI+IHNIA+ +D+ +    EE    S  ++  P ++ EYIPFW +K KE
Sbjct: 427  TSIQIQAFEGLVILIHNIADNMDREEDEGNEEDDNNSNVIKPSPVEIHEYIPFWIDKPKE 486

Query: 496  DLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRY 555
              D E W+ ++RVRKAQK+K+ IA +HFNRDEKKGL YLK + LVSDP DP A A FFR+
Sbjct: 487  --DFETWVDHIRVRKAQKRKLAIAANHFNRDEKKGLEYLKYNYLVSDPLDPMALASFFRF 546

Query: 556  THGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHR 615
            T GLDK  +G+YLGDPD+ H+ VL  FT TFEFTGM LDTALRT+LE+FRLPGE+QKI R
Sbjct: 547  TPGLDKTMIGDYLGDPDELHLSVLRSFTHTFEFTGMNLDTALRTFLESFRLPGESQKIER 606

Query: 616  ILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREIN 675
            ++EAFSERFY+ QSS+ FASKDTV +LCYSLIMLNTDQHNPQV++KMTEDEFIRNNR IN
Sbjct: 607  MIEAFSERFYDQQSSDIFASKDTVHILCYSLIMLNTDQHNPQVRRKMTEDEFIRNNRAIN 666

Query: 676  AGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSCDFDPR 735
            AG DLP++YLSELF SI+ NA  LS  SG  ++MNP++W+ELMNR+K  QPF  C FD R
Sbjct: 667  AGNDLPKEYLSELFQSIATNAFALSTHSG-PVEMNPNRWIELMNRTKTTQPFSLCQFDRR 726

Query: 736  LGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQYGLEDTLDELLAMFC 795
            +GRDMF  IAGPS+A+++AFFEH+D+DE+L+EC++ + SI ++ QYGLED LDEL+A FC
Sbjct: 727  IGRDMFATIAGPSIAAVSAFFEHSDDDEVLHECVDAMISIARVAQYGLEDILDELIASFC 786

Query: 796  KFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRL 855
            KFTTLLNPY + EETLF FSHD+KP++ATLAVFT+AN FGD+IRGGWRNIVDCLLKL++L
Sbjct: 787  KFTTLLNPYTTPEETLFAFSHDMKPRMATLAVFTLANTFGDSIRGGWRNIVDCLLKLRKL 846

Query: 856  KLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMASRFSQFLSLDSMEDS 915
            +LLPQSVI+FE+     N  + SD   +  SQD KF  +Q S +  RFS FL+LD++E+S
Sbjct: 847  QLLPQSVIEFEI--NEENGGSESDMNNV-SSQDTKFNRRQGSSLMGRFSHFLALDNVEES 906

Query: 916  LTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKFSTPVEEE 975
            + L ++E+EQNLK +KQCRIG IFS S+ + D A+LNLGRSLI+AAAGKGQKFST +EEE
Sbjct: 907  VALGMSEFEQNLKVIKQCRIGQIFSKSSVLPDVAVLNLGRSLIYAAAGKGQKFSTAIEEE 966

Query: 976  ETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLGLFKVCLT 1035
            ETV FCWDLIIT+ ++N++RF +FWPS+HEYL  V  FPLFS IPF EK + GLF+VC+ 
Sbjct: 967  ETVKFCWDLIITIALSNVHRFNMFWPSYHEYLLNVANFPLFSPIPFVEKGLPGLFRVCIK 1026

Query: 1036 LLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQSQIGWKS 1095
            +L++   D LPEELIF+S+ +MW +DKEI++TC+++IT+ VSKI+++Y ANL + IGWKS
Sbjct: 1027 ILASNLQDHLPEELIFRSLTIMWKIDKEIIETCYDTITEFVSKIIIDYSANLHTNIGWKS 1086

Query: 1096 LLHLLSATGRHPETYDQGVETLIMLMS-DASHITRTNYTFCIDCAFSYVALKNSPLDKNL 1155
            +L LLS  GRHPET +Q V+ LI LMS +ASH+++++Y +CIDCAFS+VAL+NS ++KNL
Sbjct: 1087 VLQLLSLCGRHPETKEQAVDALIGLMSFNASHLSQSSYAYCIDCAFSFVALRNSSVEKNL 1146

Query: 1156 KILDSLSDSVNFLVQWYRHYCAESGNSFSVASN-ASSSSLDDKGLGSSNFALTLFLKLGE 1215
            KILD ++DSV  LV+WY+    ++ NS+S ASN +SSSS+++  L   NF   LFLKL E
Sbjct: 1147 KILDLMADSVTMLVKWYKTASTDTANSYSPASNTSSSSSMEENNLRGVNFVHHLFLKLSE 1206

Query: 1216 ALRKTSLARREEIRNHAITSLKKSFVLA-EELDFPPTNCIGCFNNIIFAMVDDLHEKMLE 1275
            A RKT+LARREEIRN A+TSL+KSF +  E+L F P+ CI C +++IF  +DDLHEK+L+
Sbjct: 1207 AFRKTTLARREEIRNRAVTSLEKSFTMGHEDLGFTPSGCIYCIDHVIFPTIDDLHEKLLD 1266

Query: 1276 YSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCMKAD 1335
            YSRR+NAERE RSMEGTLKI+M++L +V+LVYL+QI ES  FRTFWLGVLRRMDTCMKAD
Sbjct: 1267 YSRRENAEREMRSMEGTLKIAMKVLMNVFLVYLEQIVESAEFRTFWLGVLRRMDTCMKAD 1326

Query: 1336 LGSYGESSLKELVPELLRKIITTMRGKEILVKKEGEDLWEITYIQIQWIAPGIKDELFPE 1386
            LG YG++ L+E+VPELL  +I TM+ KEILV+KE +DLWEITYIQIQWIAP +KDELFP+
Sbjct: 1327 LGEYGDNKLQEVVPELLTTMIGTMKEKEILVQKEDDDLWEITYIQIQWIAPALKDELFPD 1373

BLAST of PI0027210 vs. TAIR 10
Match: AT1G13980.1 (sec7 domain-containing protein )

HSP 1 Score: 1049.7 bits (2713), Expect = 2.1e-306
Identity = 597/1461 (40.86%), Postives = 892/1461 (61.05%), Query Frame = 0

Query: 4    RSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDSSIQ 63
            ++ ++E E            L+CM++TE+ A+LAV+RR        Y+S  DD  + S+ 
Sbjct: 11   KAIEEEPEDFECTDSSNTTTLACMIDTEIAAVLAVMRRNVR-WGGRYMSG-DDQLEHSLI 70

Query: 64   QSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFD 123
            QSLK+LR  +F   Q W TI P +Y+ P LDVI+SD+  A  T +ALS++ KI+ + + D
Sbjct: 71   QSLKALRKQVFSWNQPWHTISPMLYLQPFLDVIRSDETGAPITSIALSSVYKILNLNVID 130

Query: 124  EKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCT 183
            + T   +DA++L+V  +T+C+ E TD  +E+ V+MK+LQVL   M ++AS +L++Q VCT
Sbjct: 131  QNTANIEDAMHLVVDSVTSCRFEVTDPASEEVVLMKILQVLLACMKNKASVMLSNQHVCT 190

Query: 184  IVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIE------------------ 243
            +VNTCF VV Q+  +G+LLQR AR+TM+EL++ IFS LP++E                  
Sbjct: 191  VVNTCFRVVHQAGMKGELLQRVARHTMHELVRCIFSHLPDVERTETTLVNRAGSIKQEKA 250

Query: 244  ------------VRDGE-ESESDTE---------------DADLG-GS-----------L 303
                        V DG   SE D E               D  +G GS           +
Sbjct: 251  GVDSDYAIVSKPVEDGNANSEYDVENSMATFATGAQSLMDDGPVGPGSRKPASPYDLHIM 310

Query: 304  DSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRT----ADEDVQLFALVLINSAVEL 363
               YG+  ++++FHFLCSLLNVVE +     G+GSR+     DEDV LFAL LINSA+EL
Sbjct: 311  TEPYGVPSMVEIFHFLCSLLNVVEHV-----GMGSRSNTIAFDEDVPLFALNLINSAIEL 370

Query: 364  SGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAF 423
             G +I  HP+LL ++QD+LF +L+ +G + +PL+LSM+CS VLN+Y  LR  ++LQLEAF
Sbjct: 371  GGSSIRHHPRLLSLIQDELFRNLMQFGLSMSPLILSMVCSIVLNLYQHLRTELKLQLEAF 430

Query: 424  FIYVALKLA--SFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 483
            F  V L+LA   +G S Q QEVA+E ++NFCRQ SF++E Y N DCD    N+FEE+  L
Sbjct: 431  FSCVILRLAQGKYGPSYQQQEVAMEALVNFCRQKSFMVEMYANLDCDITCSNVFEELSNL 490

Query: 484  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 543
            L K +FP   PL+ ++I A +GL+ VI  +AE++         G   L + P  +DEY P
Sbjct: 491  LSKSTFPVNCPLSAMHILALDGLIAVIQGMAERIS-------NGLTGLDLGPVHLDEYTP 550

Query: 544  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPK 603
            FW  K     D   W+ +VR RK  K++++I   HFNRD KKGL +L+ + L+ D  DP+
Sbjct: 551  FWMVKCDNYSDPNHWVSFVRRRKYIKRRLMIGADHFNRDPKKGLEFLQGTHLLPDKLDPQ 610

Query: 604  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 663
            + A FFRYT GLDK  VG++LG+ D+F V+VL EF  TF+F  M LDTALR +LETFRLP
Sbjct: 611  SVACFFRYTAGLDKNLVGDFLGNHDEFCVQVLNEFAGTFDFQYMNLDTALRLFLETFRLP 670

Query: 664  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 723
            GE+QKI R+LEAFSER+Y +QS    A+KD   VL YS+IMLNTDQHN QVKKKMTE++F
Sbjct: 671  GESQKIQRVLEAFSERYY-MQSPEILANKDAALVLSYSIIMLNTDQHNVQVKKKMTEEDF 730

Query: 724  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQL-DMNPSKWVELMNRSKIIQP 783
            IRNNR IN G DLPR++LSELFHSI NN I  +P+ G    +M PS+W++LM++SK   P
Sbjct: 731  IRNNRHINGGNDLPREFLSELFHSICNNEIRTTPEQGAGFPEMTPSRWIDLMHKSKKTAP 790

Query: 784  FMSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQ-YGLED 843
            ++  D    L  DMF  ++GP++A+++  F+HA+ +++   CI+G  +I KI+  + LED
Sbjct: 791  YILADSRAYLDHDMFAIMSGPTIAAISVVFDHAEHEDVYQTCIDGFLAIAKISACHHLED 850

Query: 844  TLDELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNI 903
             LD+L+   CKFTTLLNP +S +E +  F  D K ++AT+ +FTIAN +GD IR GWRNI
Sbjct: 851  VLDDLVVSLCKFTTLLNP-SSVDEPVLAFGDDAKARMATITIFTIANKYGDYIRTGWRNI 910

Query: 904  VDCLLKLKRLKLLPQSVI-DFEVASTSSND------VARSDSGVIFPSQDPKFCTQQSSG 963
            +DC+L+L +L LLP  V  D    S  S++      +A S S     S       ++SSG
Sbjct: 911  LDCILRLHKLGLLPARVASDAADESEHSSEQGQGKPLANSLSSAHLQSMG---TPRRSSG 970

Query: 964  MASRFSQFLSLDSME---DSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGR 1023
            +  RFSQ LSLD+ E         L  +++ L+ +++C I +IF+ S  +  E+LL L R
Sbjct: 971  LMGRFSQLLSLDTEEPRSQPTEQQLAAHQRTLQTIQKCHIDSIFTESKFLQAESLLQLAR 1030

Query: 1024 SLIFAAAGKGQKFSTPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPL 1083
            +LI+ AAG+ QK ++  E+E+T  FC +L+I +T+ N  R  + W   +E++ T+ Q  +
Sbjct: 1031 ALIW-AAGRPQKGTSSPEDEDTAVFCLELLIAITLNNRDRIVLLWQGVYEHIATIAQSTV 1090

Query: 1084 FSAIPFAEKAVLGLFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQS 1143
                   +KA+ GL ++C  LL     + L +EL+ +S+ L+  LD  + D   E I   
Sbjct: 1091 MPC-NLVDKAIFGLLRICQRLLP--YKESLADELL-RSLQLVLKLDARVADAYCEQIAIE 1150

Query: 1144 VSKILVEYPANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFC 1203
            VS+++     +++SQ GW+++  LLS T RHPE  + G + +  +MS+ +H+   NY  C
Sbjct: 1151 VSRLVKANANHIRSQAGWRTITSLLSITARHPEASESGFDAVSFVMSEGTHLYPANYVLC 1210

Query: 1204 IDCAFSYVALKNSPLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDD 1263
            +D A  +   +    +++++ LD + DS+ FL +W          + S   N      +D
Sbjct: 1211 VDAARQFAESRVGQSERSIRALDLMGDSLEFLAKW----------ALSAKENMGE---ED 1270

Query: 1264 KGLGSSNFALTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCF 1323
             G  S +    ++L+L + LRK  L +RE++RNHA+ SL+K     + ++   +    CF
Sbjct: 1271 FGKMSQDIG-EMWLRLVQGLRKVCLDQREDVRNHALQSLQKCLGGVDGINLAHSMWSQCF 1330

Query: 1324 NNIIFAMVDDLHEKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFR 1383
            + +IF ++DDL    LE +    ++++ R+MEGTL ++++LL+ V+L  L+++S+   F 
Sbjct: 1331 DKVIFTVLDDL----LEIAA--GSQKDYRNMEGTLLLAIKLLSKVFLQQLQELSQLSTFC 1390

Query: 1384 TFWLGVLRRMDTCMKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKE---GEDLWE 1386
              WLGVL RM+  MK  +       L+E VPELL+ I+  M+ K +L+++    G+ LWE
Sbjct: 1391 KLWLGVLTRMEKYMKVKVRGKKSDKLQESVPELLKNILLVMKTKGVLLQRSALGGDSLWE 1427

BLAST of PI0027210 vs. TAIR 10
Match: AT1G13980.2 (sec7 domain-containing protein )

HSP 1 Score: 1049.7 bits (2713), Expect = 2.1e-306
Identity = 597/1461 (40.86%), Postives = 892/1461 (61.05%), Query Frame = 0

Query: 4    RSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDSSIQ 63
            ++ ++E E            L+CM++TE+ A+LAV+RR        Y+S  DD  + S+ 
Sbjct: 11   KAIEEEPEDFECTDSSNTTTLACMIDTEIAAVLAVMRRNVR-WGGRYMSG-DDQLEHSLI 70

Query: 64   QSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFD 123
            QSLK+LR  +F   Q W TI P +Y+ P LDVI+SD+  A  T +ALS++ KI+ + + D
Sbjct: 71   QSLKALRKQVFSWNQPWHTISPMLYLQPFLDVIRSDETGAPITSIALSSVYKILNLNVID 130

Query: 124  EKTPGAKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCT 183
            + T   +DA++L+V  +T+C+ E TD  +E+ V+MK+LQVL   M ++AS +L++Q VCT
Sbjct: 131  QNTANIEDAMHLVVDSVTSCRFEVTDPASEEVVLMKILQVLLACMKNKASVMLSNQHVCT 190

Query: 184  IVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIE------------------ 243
            +VNTCF VV Q+  +G+LLQR AR+TM+EL++ IFS LP++E                  
Sbjct: 191  VVNTCFRVVHQAGMKGELLQRVARHTMHELVRCIFSHLPDVERTETTLVNRAGSIKQEKA 250

Query: 244  ------------VRDGE-ESESDTE---------------DADLG-GS-----------L 303
                        V DG   SE D E               D  +G GS           +
Sbjct: 251  GVDSDYAIVSKPVEDGNANSEYDVENSMATFATGAQSLMDDGPVGPGSRKPASPYDLHIM 310

Query: 304  DSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRT----ADEDVQLFALVLINSAVEL 363
               YG+  ++++FHFLCSLLNVVE +     G+GSR+     DEDV LFAL LINSA+EL
Sbjct: 311  TEPYGVPSMVEIFHFLCSLLNVVEHV-----GMGSRSNTIAFDEDVPLFALNLINSAIEL 370

Query: 364  SGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAF 423
             G +I  HP+LL ++QD+LF +L+ +G + +PL+LSM+CS VLN+Y  LR  ++LQLEAF
Sbjct: 371  GGSSIRHHPRLLSLIQDELFRNLMQFGLSMSPLILSMVCSIVLNLYQHLRTELKLQLEAF 430

Query: 424  FIYVALKLA--SFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 483
            F  V L+LA   +G S Q QEVA+E ++NFCRQ SF++E Y N DCD    N+FEE+  L
Sbjct: 431  FSCVILRLAQGKYGPSYQQQEVAMEALVNFCRQKSFMVEMYANLDCDITCSNVFEELSNL 490

Query: 484  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 543
            L K +FP   PL+ ++I A +GL+ VI  +AE++         G   L + P  +DEY P
Sbjct: 491  LSKSTFPVNCPLSAMHILALDGLIAVIQGMAERIS-------NGLTGLDLGPVHLDEYTP 550

Query: 544  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPK 603
            FW  K     D   W+ +VR RK  K++++I   HFNRD KKGL +L+ + L+ D  DP+
Sbjct: 551  FWMVKCDNYSDPNHWVSFVRRRKYIKRRLMIGADHFNRDPKKGLEFLQGTHLLPDKLDPQ 610

Query: 604  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 663
            + A FFRYT GLDK  VG++LG+ D+F V+VL EF  TF+F  M LDTALR +LETFRLP
Sbjct: 611  SVACFFRYTAGLDKNLVGDFLGNHDEFCVQVLNEFAGTFDFQYMNLDTALRLFLETFRLP 670

Query: 664  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 723
            GE+QKI R+LEAFSER+Y +QS    A+KD   VL YS+IMLNTDQHN QVKKKMTE++F
Sbjct: 671  GESQKIQRVLEAFSERYY-MQSPEILANKDAALVLSYSIIMLNTDQHNVQVKKKMTEEDF 730

Query: 724  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQL-DMNPSKWVELMNRSKIIQP 783
            IRNNR IN G DLPR++LSELFHSI NN I  +P+ G    +M PS+W++LM++SK   P
Sbjct: 731  IRNNRHINGGNDLPREFLSELFHSICNNEIRTTPEQGAGFPEMTPSRWIDLMHKSKKTAP 790

Query: 784  FMSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKITQ-YGLED 843
            ++  D    L  DMF  ++GP++A+++  F+HA+ +++   CI+G  +I KI+  + LED
Sbjct: 791  YILADSRAYLDHDMFAIMSGPTIAAISVVFDHAEHEDVYQTCIDGFLAIAKISACHHLED 850

Query: 844  TLDELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNI 903
             LD+L+   CKFTTLLNP +S +E +  F  D K ++AT+ +FTIAN +GD IR GWRNI
Sbjct: 851  VLDDLVVSLCKFTTLLNP-SSVDEPVLAFGDDAKARMATITIFTIANKYGDYIRTGWRNI 910

Query: 904  VDCLLKLKRLKLLPQSVI-DFEVASTSSND------VARSDSGVIFPSQDPKFCTQQSSG 963
            +DC+L+L +L LLP  V  D    S  S++      +A S S     S       ++SSG
Sbjct: 911  LDCILRLHKLGLLPARVASDAADESEHSSEQGQGKPLANSLSSAHLQSMG---TPRRSSG 970

Query: 964  MASRFSQFLSLDSME---DSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGR 1023
            +  RFSQ LSLD+ E         L  +++ L+ +++C I +IF+ S  +  E+LL L R
Sbjct: 971  LMGRFSQLLSLDTEEPRSQPTEQQLAAHQRTLQTIQKCHIDSIFTESKFLQAESLLQLAR 1030

Query: 1024 SLIFAAAGKGQKFSTPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPL 1083
            +LI+ AAG+ QK ++  E+E+T  FC +L+I +T+ N  R  + W   +E++ T+ Q  +
Sbjct: 1031 ALIW-AAGRPQKGTSSPEDEDTAVFCLELLIAITLNNRDRIVLLWQGVYEHIATIAQSTV 1090

Query: 1084 FSAIPFAEKAVLGLFKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQS 1143
                   +KA+ GL ++C  LL     + L +EL+ +S+ L+  LD  + D   E I   
Sbjct: 1091 MPC-NLVDKAIFGLLRICQRLLP--YKESLADELL-RSLQLVLKLDARVADAYCEQIAIE 1150

Query: 1144 VSKILVEYPANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFC 1203
            VS+++     +++SQ GW+++  LLS T RHPE  + G + +  +MS+ +H+   NY  C
Sbjct: 1151 VSRLVKANANHIRSQAGWRTITSLLSITARHPEASESGFDAVSFVMSEGTHLYPANYVLC 1210

Query: 1204 IDCAFSYVALKNSPLDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDD 1263
            +D A  +   +    +++++ LD + DS+ FL +W          + S   N      +D
Sbjct: 1211 VDAARQFAESRVGQSERSIRALDLMGDSLEFLAKW----------ALSAKENMGE---ED 1270

Query: 1264 KGLGSSNFALTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCF 1323
             G  S +    ++L+L + LRK  L +RE++RNHA+ SL+K     + ++   +    CF
Sbjct: 1271 FGKMSQDIG-EMWLRLVQGLRKVCLDQREDVRNHALQSLQKCLGGVDGINLAHSMWSQCF 1330

Query: 1324 NNIIFAMVDDLHEKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFR 1383
            + +IF ++DDL    LE +    ++++ R+MEGTL ++++LL+ V+L  L+++S+   F 
Sbjct: 1331 DKVIFTVLDDL----LEIAA--GSQKDYRNMEGTLLLAIKLLSKVFLQQLQELSQLSTFC 1390

Query: 1384 TFWLGVLRRMDTCMKADLGSYGESSLKELVPELLRKIITTMRGKEILVKKE---GEDLWE 1386
              WLGVL RM+  MK  +       L+E VPELL+ I+  M+ K +L+++    G+ LWE
Sbjct: 1391 KLWLGVLTRMEKYMKVKVRGKKSDKLQESVPELLKNILLVMKTKGVLLQRSALGGDSLWE 1427

BLAST of PI0027210 vs. TAIR 10
Match: AT5G39500.1 (GNOM-like 1 )

HSP 1 Score: 933.3 bits (2411), Expect = 2.2e-271
Identity = 544/1447 (37.60%), Postives = 848/1447 (58.60%), Query Frame = 0

Query: 17   SKRRELGLSCMLNTEVGAILAVIRRPPSDLNSPYISTIDDTYDSSIQQSLKSLRALIFHP 76
            SK  +  ++ M+N+E+GA+LAV+RR        YI+  DD  + S+  SLK LR  IF  
Sbjct: 22   SKPSKGAVASMINSEIGAVLAVMRRNVR-WGVRYIAD-DDQLEHSLIHSLKELRKQIFSW 81

Query: 77   QQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLI 136
            Q  W+ +DP +YI P LDVI SD+  A  TGVALS++ KI+ +E+F  +T    +A+++I
Sbjct: 82   QSNWQYVDPRLYIQPFLDVILSDETGAPITGVALSSVYKILTLEVFTLETVNVGEAMHII 141

Query: 137  VLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSA 196
            V  + +C+ E TD  +E+ V+MK+LQVL   +  +AS  L++Q +CTIVNTC  VV QS+
Sbjct: 142  VDAVKSCRFEVTDPASEEVVLMKILQVLLACVKSKASNGLSNQDICTIVNTCLRVVHQSS 201

Query: 197  SRGDLLQRTARYTMNELIQIIFSRL----------------------------------- 256
            S+ +LLQR AR+TM+ELI+ IFS+L                                   
Sbjct: 202  SKSELLQRIARHTMHELIRCIFSQLPFISPLANECELHVDNKVGTVDWDPNSGEKRVENG 261

Query: 257  ----------------------PEIEVRDGEESESDTEDADLGGSLDSG----YGIRCVI 316
                                  PE ++R+ E+    ++D +   + ++     YGI C++
Sbjct: 262  NIASISDTLGTDKDDPSSEMVIPETDLRNDEKKTEVSDDLNAAANGENAMMAPYGIPCMV 321

Query: 317  DVFHFLCSLLNVVEMLEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRM 376
            ++FHFLC+LLNV E  EV          DEDV LFAL LINSA+EL G +  +HPKLL +
Sbjct: 322  EIFHFLCTLLNVGENGEVNSRS-NPIAFDEDVPLFALGLINSAIELGGPSFREHPKLLTL 381

Query: 377  VQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFIYVALKLA--SFG 436
            +QDDLF +L+ +G + +PL+LS +CS VLN+Y  LR  +++QLEAFF YV L++A    G
Sbjct: 382  IQDDLFCNLMQFGMSMSPLILSTVCSIVLNLYLNLRTELKVQLEAFFSYVLLRIAQSKHG 441

Query: 437  NSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKLLCKLSFPTGSPLTT 496
            +S Q QEVA+E +++ CRQ +FI E + N+DCD    N+FE++  LL K +FP   PL+ 
Sbjct: 442  SSYQQQEVAMEALVDLCRQHTFIAEVFANFDCDITCSNVFEDVSNLLSKNAFPVNGPLSA 501

Query: 497  LNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIPFWEEKSKEDLDLED 556
            ++I A +GL+ ++  +AE++ +    +          P   + Y  FW  + +   D   
Sbjct: 502  MHILALDGLISMVQGMAERVGEELPAS--------DVPTHEERYEEFWTVRCENYGDPNF 561

Query: 557  WLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRYTHGLDK 616
            W+ +VR  K  KKK+++    FNRD  KGL YL+   L+ +  DPK+ A FFRYT GLDK
Sbjct: 562  WVPFVRKVKHIKKKLMLGADRFNRDPNKGLQYLQGVHLLPEKLDPKSVACFFRYTCGLDK 621

Query: 617  QFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFS 676
              +G++LG+ DQF ++VL EF +TF+F  M L TALR ++ TF+L GEAQKIHR+LEAFS
Sbjct: 622  NVMGDFLGNHDQFCIQVLHEFAKTFDFQNMNLATALRLFVGTFKLSGEAQKIHRVLEAFS 681

Query: 677  ERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREINAGKDLP 736
            ER+YE QS +    KD  FVL YS+I+LNTDQHN QVK +MTE++FIRNNR IN G DLP
Sbjct: 682  ERYYE-QSPHILIDKDAAFVLAYSIILLNTDQHNAQVKTRMTEEDFIRNNRTINGGADLP 741

Query: 737  RDYLSELFHSISNNAIILSPQ--SGLQLDMNPSKWVELMNRSKIIQPFMSCDFDPRLGRD 796
            R+YLSE++HSI ++ I +     +G QL M  S+W+ ++ +SK   P++ CD    L RD
Sbjct: 742  REYLSEIYHSIRHSEIQMDEDKGTGFQL-MTASRWISVIYKSKETSPYIQCDAASHLDRD 801

Query: 797  MFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSITKIT-QYGLEDTLDELLAMFCKFT 856
            MF  ++GP++A+ +  FE A+++++L  CI+GL +I K++  Y L   LD+L+   CKFT
Sbjct: 802  MFYIVSGPTIAATSVVFEQAEQEDVLRRCIDGLLAIAKLSAYYHLNSVLDDLVVSLCKFT 861

Query: 857  TLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLL 916
                P  SA+E + V   D + ++AT AVF IAN +GD I  GW+NI++C+L L +L +L
Sbjct: 862  PFFAP-LSADEAVLVLGEDARARMATEAVFLIANKYGDYISAGWKNILECVLSLNKLHIL 921

Query: 917  PQSVI-----DFEVASTSSNDVARSDSGVIFPSQ-DPKFCTQQSSGMASRFSQFLSLDSM 976
            P  +      D E+++++      S + V   SQ  P    ++SS    RF   LS DS 
Sbjct: 922  PDHIASDAADDPELSTSNLEQEKPSANPVPVVSQSQPSAMPRKSSSFIGRF--LLSFDSE 981

Query: 977  EDSLTLNLNE---YEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKFS 1036
            E     +  E   Y+     VK C I +IFS+S  +  E+L  L  SLI  A+GK     
Sbjct: 982  ETKPLPSEEELAAYKHARGIVKDCHIDSIFSDSKFLQAESLQQLVNSLI-RASGK----- 1041

Query: 1037 TPVEEEETVGFCWDLIITMTMANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLGL 1096
                +E +  FC +L+I +T+ N  R  + WP+ +E++  +VQ  L +     EKAV G+
Sbjct: 1042 ----DEASSVFCLELLIAVTLNNRDRILLIWPTVYEHILGIVQLTL-TPCTLVEKAVFGV 1101

Query: 1097 FKVCLTLLSTYQPDKLPEELIFKSINLMWMLDKEILDTCFESITQSVSKILVEYPANLQS 1156
             K+C  LL     + L +EL+ KS+ L+  L  ++ D   E I Q V +++    ++++S
Sbjct: 1102 LKICQRLLP--YKENLTDELL-KSLQLVLKLKAKVADAYCERIAQEVVRLVKANASHVRS 1161

Query: 1157 QIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNSP 1216
            + GW++++ LLS T RHPE  + G E L  +MS+ +H+  +NY  C+D A  +   +   
Sbjct: 1162 RTGWRTIISLLSITARHPEASEAGFEALRFIMSEGAHLLPSNYELCLDAASHFAESRVGE 1221

Query: 1217 LDKNLKILDSLSDSVNFLVQWYRHYCAESGNSFSVASNASSSSLDDKGLGSSNFALTLFL 1276
            +D+++  +D +S+SV  L +W +    E+ NS    ++A     +D G         ++L
Sbjct: 1222 VDRSISAIDLMSNSVFCLARWSQ----EAKNSIG-ETDAMMKLSEDIG--------KMWL 1281

Query: 1277 KLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHEK 1336
            KL + L+K  L +R+E+RNHAI+ L+++   A+ +  P      CF++ +F ++DD    
Sbjct: 1282 KLVKNLKKVCLDQRDEVRNHAISMLQRAIAGADGIMLPQPLWFQCFDSAVFILLDD---- 1341

Query: 1337 MLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCM 1386
            +L +S  ++ +   +++E TL ++ +L++  +L  L+ IS+ P F   W+GVL R++T M
Sbjct: 1342 VLTFSIENSRKTLKKTVEETLVLATKLMSKAFLQSLQDISQQPSFCRLWVGVLNRLETYM 1401

BLAST of PI0027210 vs. TAIR 10
Match: AT1G01960.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 245.7 bits (626), Expect = 2.1e-64
Identity = 295/1185 (24.89%), Postives = 499/1185 (42.11%), Query Frame = 0

Query: 225  EVRDGEESESDTEDADLGGSLDSGYGIRCVIDVFHFLCSLLNVVEMLEVGDGGLGSRTAD 284
            E+ DGE  + D  +  +G  L      R    VF  LC L       E  +   G     
Sbjct: 316  ELADGEVEKDDDSEVQIGNKLR-----RDAFLVFRALCKLSMKTPPKEDPELMRG----- 375

Query: 285  EDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVL 344
               ++ AL L+   +E +G       + L  ++  L   L+   A++  ++  + CS +L
Sbjct: 376  ---KIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSASNLMIIFQLSCSILL 435

Query: 345  NIYHFLRRFVRLQLEAFFIYVALKLASFGNSTQIQE--VALEGIINFCRQSSFILEFYVN 404
            ++    R  ++ ++  FF  + L++         Q+  + L  +   C  S  +++ ++N
Sbjct: 436  SLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPDFQQKMIVLRFLDKLCVDSQILVDIFIN 495

Query: 405  YDCDPLRWNLFEEI--GKLLCKLSFPTGSPLTTL-------NIQAFEGLVIVIHNIAEKL 464
            YDCD    N+FE +  G L      P G+  T L        ++A + LV V+ ++ + +
Sbjct: 496  YDCDVNSSNIFERMVNGLLKTAQGVPPGTVTTLLPPQEAAMKLEAMKCLVAVLRSMGDWV 555

Query: 465  DKHKEETCGGSGN-LRVYPAQVDE-------------YIPFWEEKSKEDLDL--EDWLRY 524
            +K        S   L +    ++E             +  F    S+ +L     D L  
Sbjct: 556  NKQLRLPDPYSAKMLEIVDRNLEEGSHPVENGKGDGGHGGFERSDSQSELSSGNSDALA- 615

Query: 525  VRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDPPDPKAYAYFFRYTHGLDKQFVG 584
            +  R+A K ++      FN+  KKG+ +L  +  V D   P+  A F +   GL+K  +G
Sbjct: 616  IEQRRAYKLELQEGISIFNQKPKKGIEFLIKANKVGD--SPEEIAAFLKDASGLNKTLIG 675

Query: 585  EYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFSERFY 644
            +YLG+ +   +KV+  + ++FEF GM  D A+R +L  FRLPGEAQKI RI+E F+ERF 
Sbjct: 676  DYLGEREDLSLKVMHAYVDSFEFQGMEFDEAIRAFLRGFRLPGEAQKIDRIMEKFAERFC 735

Query: 645  ELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREINAGKDLPRDYL 704
            +    + F+S DT +VL YS+I+LNTD HNP VK KMT D FIRNNR I+ GKDLP +YL
Sbjct: 736  KCNPKD-FSSADTAYVLAYSVILLNTDAHNPMVKSKMTADGFIRNNRGIDDGKDLPEEYL 795

Query: 705  SELFHSISNNAI----------------------------ILSPQSGLQLDMNPS----- 764
              L+  IS N I                            I+ P+ G  ++M  S     
Sbjct: 796  RALYERISRNEIKMKDDGLGPQQKQPTNSSRLLGLDTILNIVVPRRGDDMNMETSDDLIR 855

Query: 765  ----KWVELMNRSKIIQPFMSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNEC 824
                ++ E   +S+ +    S   D  + R M      P +A+ +   + +D+  +   C
Sbjct: 856  HMQERFKEKARKSESVYYAAS---DVIILRFMVEVCWAPMLAAFSVPLDQSDDAVITTLC 915

Query: 825  IEGLFSITKITQ-YGLEDTLDELLAMFCKFTTLLNPYASAEETLFVFSHDLKPK--LATL 884
            +EG      +T    L+   D  +    KFT+L +P             D+K K   A  
Sbjct: 916  LEGFHHAIHVTSVMSLKTHRDAFVTSLAKFTSLHSP------------ADIKQKNIEAIK 975

Query: 885  AVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLL----PQSVIDFEVASTSSND--VARSD 944
            A+  +A   G+ ++  W +I+ C+ + + L LL    P     F    T S +  +A+ +
Sbjct: 976  AIVKLAEEEGNYLQDAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQTESGNSPLAKPN 1035

Query: 945  SGVIFPSQDPKFC----------TQQSSGMASRFSQFLSLDSMEDSLTLNLNEYEQNLKF 1004
            S      + P             +   SG+A + S  ++ + M ++L  NLN  EQ    
Sbjct: 1036 SVPAIKERAPGKLQYAASAMIRGSYDGSGVAGKASNTVTSEQM-NNLISNLNLLEQ---- 1095

Query: 1005 VKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQKFSTPVEEEETVGFCWDLIITMT 1064
                 +  IF+ S  +  EA+++  ++L   +  + +  S P        F    I+ + 
Sbjct: 1096 --VGDMSRIFTRSQRLNSEAIIDFVKALCKVSMDELRSPSDPRV------FSLTKIVEIA 1155

Query: 1065 MANIYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVLGLFKV-CLTLLSTYQPDKLPEE 1124
              N+ R ++ W S    L        F  I  ++   + +F +  L  LS    ++  EE
Sbjct: 1156 HYNMNRIRLVWSSIWHVLS-----DFFVTIGCSDNLSIAIFAMDSLRQLSMKFLER--EE 1215

Query: 1125 LIFKSINLMWM------LDKEILDTCFESITQSVSKILVEYPANLQSQIGWKSLLHLL-- 1184
            L   +    +M      + K       E I + VS++++    N++S  GWKS+  +   
Sbjct: 1216 LANYNFQNEFMKPFVVVMRKSGAVEIRELIIRCVSQMVLSRVDNVKS--GWKSMFMIFTT 1275

Query: 1185 SATGRHPETYDQGVETLIMLMSD-ASHITRTNYTFCIDCAFSYVALKNSPLDKNLKILDS 1244
            +A   H        E +  ++ D   HIT T  T   DC    VA  N   +K++ +   
Sbjct: 1276 AAHDAHKNIVFLSFEMVEKIIRDYFPHITETETTTFTDCVNCLVAFTNCKFEKDISL--- 1335

Query: 1245 LSDSVNFLVQWYRHYCAESGNSFSVASNASSS-------SLDDKGLGSSNFALTLFLKLG 1302
               ++ FL Q+     AE     S+  N   S         D      S+  L  +  L 
Sbjct: 1336 --QAIAFL-QYCARKLAEGYVGSSLRRNPPLSPQGGKIGKQDSGKFLESDEHLYSWFPLL 1395

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4K2K30.0e+0066.11ARF guanine-nucleotide exchange factor GNL2 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q425103.0e-30540.86ARF guanine-nucleotide exchange factor GNOM OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q9FLY53.1e-27037.60ARF guanine-nucleotide exchange factor GNL1 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q9R1D71.3e-9022.29Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Cr... [more]
Q925384.1e-8924.47Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Ho... [more]
Match NameE-valueIdentityDescription
A0A5A7V0610.0e+0098.05ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C8R10.0e+0097.98ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo OX=3656 GN=LOC103497... [more]
A0A0A0LF220.0e+0097.69SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G004740 PE=4 S... [more]
A0A6J1FBD60.0e+0090.48ARF guanine-nucleotide exchange factor GNL2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1DTF70.0e+0089.91ARF guanine-nucleotide exchange factor GNL2 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
KAA0061562.10.0e+0098.05ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa] >TYK10710... [more]
XP_008458277.10.0e+0097.98PREDICTED: ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo][more]
XP_004139429.10.0e+0097.69ARF guanine-nucleotide exchange factor GNL2 [Cucumis sativus] >KGN60625.1 hypoth... [more]
XP_038889761.10.0e+0095.25ARF guanine-nucleotide exchange factor GNL2 [Benincasa hispida][more]
KAG6586424.10.0e+0090.48ARF guanine-nucleotide exchange factor GNL2, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT5G19610.10.0e+0066.11GNOM-like 2 [more]
AT1G13980.12.1e-30640.86sec7 domain-containing protein [more]
AT1G13980.22.1e-30640.86sec7 domain-containing protein [more]
AT5G39500.12.2e-27137.60GNOM-like 1 [more]
AT1G01960.12.1e-6424.89SEC7-like guanine nucleotide exchange family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000904Sec7 domainSMARTSM00222sec7_5coord: 501..689
e-value: 1.0E-74
score: 264.2
IPR000904Sec7 domainPFAMPF01369Sec7coord: 505..689
e-value: 3.7E-66
score: 222.3
IPR000904Sec7 domainPROSITEPS50190SEC7coord: 502..687
score: 37.577663
IPR000904Sec7 domainCDDcd00171Sec7coord: 514..689
e-value: 1.86219E-75
score: 246.366
IPR032629Mon2, dimerisation and cyclophilin-binding domainPFAMPF16213DCBcoord: 105..221
e-value: 3.9E-8
score: 33.2
NoneNo IPR availableGENE3D1.10.220.20coord: 498..579
e-value: 1.3E-20
score: 75.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR10663GUANYL-NUCLEOTIDE EXCHANGE FACTORcoord: 12..1385
NoneNo IPR availablePANTHERPTHR10663:SF322ARF GUANINE-NUCLEOTIDE EXCHANGE FACTOR GNL2coord: 12..1385
IPR032691Guanine nucleotide exchange factor, N-terminalPFAMPF12783Sec7_Ncoord: 251..409
e-value: 4.7E-26
score: 91.6
IPR023394Sec7, C-terminal domain superfamilyGENE3D1.10.1000.11coord: 580..696
e-value: 5.8E-45
score: 154.4
IPR035999Sec7 domain superfamilySUPERFAMILY48425Sec7 domaincoord: 501..691

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027210.1PI0027210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009846 pollen germination
biological_process GO:0015031 protein transport
biological_process GO:0032012 regulation of ARF protein signal transduction
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005829 cytosol
cellular_component GO:0016020 membrane
cellular_component GO:0090406 pollen tube
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity