PI0027146 (gene) Melon (PI 482460) v1

Overview
NamePI0027146
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionReverse transcriptase
Locationchr02: 16460270 .. 16462476 (+)
RNA-Seq ExpressionPI0027146
SyntenyPI0027146
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTCGCCTATACACATTACAGAAATAGGTTCATGAGTGAAAGTGACTCGGTCAGAGACCGATTCCCTCCCTTATGGAGGTTTGTTTTGAAGTTCGACTTGAGGAGGATCGTACAAGTGCTATGAATGTTTTGACAACCCCTTCTATTGACTCCTCTGCTTTAGTGCGAGATCCTCCACCATTGGCAATGAAAAGAACAATGGGAAACCAGTTCCAGTCTGTGATTATTGCAAGAAACAATGGCACACCAAGGAGCAGTGTTGGAAGTTACATGGCCGTCCCCCGGGAGGTAAAAAACTTTCTTCCAATGACAAACAGAATACAGGACGGGTATATGTGAGTGAGTCTACTAGAACTTCTCAACCACCTGATTTACTTGACAACCCGATCACTCCCAGTCCTACCACTCTAGGTGCCATTGCTCAATTAGGTATACCTAAGTCCTTCGGTCTTGTTAGTCTTGATAGGAAGAAACCGCGGATTCTGGATTCTGGTGCTACAGACCATTTGACTGGTTCCTCTGAACTTTTTGTGTCCTATGTTCCATGTGCTTATAACGAAACAATTAAGATTGCAAATGGCTCCTCGACACCCATTGTGGGAAAGGGGATAATTTTTTCCTGTGAAGGGCTCTCCTTACATCATGTTTTACATGTGTCCAAAATTTCTTATAATCTGCTATCTATAAGCAAGATTACTCGTGATCTCAACTGTAAAGCGACGTTCTTACCTGATTCTGTTTCTTTTCAGGACTTGAGCTCGGGGAGGATGATTGGCACTGCCCGACATAGTAAGGGACTCTACCTCCTTGATGATGACACCTCTTCTAGTAGCACTTCTAGGACTAGTCTTTTATCTTCCTATTTTACTAATTCTGAACAAGATTGTATGTTGTGACATTTCCGCTTAGGACACCCTAATTTTCAATATATGAAACATTTATTCCCTCATCTTTTCTCTAAAATTGATGTGTCTACTTTATTTTGTGATGTGTGGATTCGGGCTAAGTGACATCGGGTATCTTTTCCTTCTCAACCATATAAACCAACCATCCTTTCACCCTTGTTCATAGTGATGTTTGGGGTCCTTCTAAGATCACTACTTCTTCTGGAAACGATGGTTTGTAACCTTTATTGATGCGTCTCACTTGGGTATTCCTCGTCTCTGACAAATCTGAGGTCACTTCCGTCTTCCAAGACTTTTATCACACCATAGAGACACAGTTCAATACAAAAATTACTATTTTATGAAGTGATAATGGTTGCGAGTTCCAAAATCACACCCTCAATGCGTTTTTTTCCTCCAAAGGGATTGTCCACCAAAGTTCCTATGCCTACACCCCTCAACAAAATGGCGTAGCCGAACGTAAAAATCGTCACCTTTTGGAAGTAGCTCGTTCACTTATGTTGTCTACTTCTCTACCGTCCTATCTCGGGGGCGATGTCGTTCTTACTGCAACCCATCTTATCAATAGGATGTCTTCCCATGTTTTACATCTCAAGACCCCTTAGAATGTCTTAAAGAGTCATATCCCTCCGCCGTCTCATTTCTGATGTTCCTCTTCATGTTTTTGGGTGCACAACCTATGTTCATCATCTTGGTCCTAACCCAACCAAGTTTACCCTTCGAGCCCAAACATGTGTCTTTGTTGGTTACCCTCTGCACCAACGAGGCTATAAATGCTTTCATCCGTCTTCTCGTAAGTACTTTGTTTCCATGGACGTCACCTTTCTTGAGGATCATCATTTCTTTCTCGTTAGCCTACTTCAGGGGGAGAGTGATAGTAGTGAGAATGAAGAGTCTAACTGGGTGGTTTCCTTAGAGTCTACCAGTCCTACTTTTGCTACCCTACCTAGCCCCGATCCTTACAACACAGTCCTACCTACGAACCAAGTTCCTTGGAAGACCTACTATAGGAGGAATCTTAGAAAGGATGTTGAGTTCCCTACAACTCAACCAGCTCCAGTCTAAGATTCTAAACCTCTGCTATCAAGGTATGACGGATACTATTGACTCACATATTGACATCAAAATGAGTGAAAGTGACAGGTCCGAGACAGCTATCCCTAGGAACATTGGTGAACAGAACAGTGTTGAAACTGGGGTCATTTCAGACAGAGAAGGCAGTGATGGTGGGGCTGAAGTCATTGCAAGAGTTACTGAGGATAACACTAGGGAGGATCGTTCAGAAAAAAATCAATAA

mRNA sequence

ATGCCTCTCGCCTATACACATTACAGAAATAGGTTCATGAGTGAAAGTGACTCGGTCAGAGACCGATTCCCTCCCTTATGGAGGTTTGTTTTGAAGTTCGACTTGAGGAGGATCGTACAAAATACAGGACGGGTATATGTGAGTGAGTCTACTAGAACTTCTCAACCACCTGATTTACTTGACAACCCGATCACTCCCAGTCCTACCACTCTAGGTGCCATTGCTCAATTAGCCCCGATCCTTACAACACAGTCCTACCTACGAACCAAGTTCCTTGGAAGACCTACTATAGGAGGAATCTTAGAAAGGATGTTGAGTTCCCTACAACTCAACCAGCTCCAGTCTAAGATTCTAAACCTCTGCTATCAAGGTATGACGGATACTATTGACTCACATATTGACATCAAAATGAGTGAAAGTGACAGGTCCGAGACAGCTATCCCTAGGAACATTGGTGAACAGAACAGTGTTGAAACTGGGGTCATTTCAGACAGAGAAGGCAGTGATGGTGGGGCTGAAGTCATTGCAAGAGTTACTGAGGATAACACTAGGGAGGATCGTTCAGAAAAAAATCAATAA

Coding sequence (CDS)

ATGCCTCTCGCCTATACACATTACAGAAATAGGTTCATGAGTGAAAGTGACTCGGTCAGAGACCGATTCCCTCCCTTATGGAGGTTTGTTTTGAAGTTCGACTTGAGGAGGATCGTACAAAATACAGGACGGGTATATGTGAGTGAGTCTACTAGAACTTCTCAACCACCTGATTTACTTGACAACCCGATCACTCCCAGTCCTACCACTCTAGGTGCCATTGCTCAATTAGCCCCGATCCTTACAACACAGTCCTACCTACGAACCAAGTTCCTTGGAAGACCTACTATAGGAGGAATCTTAGAAAGGATGTTGAGTTCCCTACAACTCAACCAGCTCCAGTCTAAGATTCTAAACCTCTGCTATCAAGGTATGACGGATACTATTGACTCACATATTGACATCAAAATGAGTGAAAGTGACAGGTCCGAGACAGCTATCCCTAGGAACATTGGTGAACAGAACAGTGTTGAAACTGGGGTCATTTCAGACAGAGAAGGCAGTGATGGTGGGGCTGAAGTCATTGCAAGAGTTACTGAGGATAACACTAGGGAGGATCGTTCAGAAAAAAATCAATAA

Protein sequence

MPLAYTHYRNRFMSESDSVRDRFPPLWRFVLKFDLRRIVQNTGRVYVSESTRTSQPPDLLDNPITPSPTTLGAIAQLAPILTTQSYLRTKFLGRPTIGGILERMLSSLQLNQLQSKILNLCYQGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDNTREDRSEKNQ
Homology
[Close]
BLAST of PI0027146 vs. ExPASy TrEMBL
Match: A0A5A7VI58 (tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold40G00780 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.7e-15
Identity = 48/67 (71.64%), Postives = 57/67 (85.07%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           QG+TDTIDSHID KMSESD+ ET IP NIGE  +V+TGV+S+REGSDGG EVIA+VTE+N
Sbjct: 425 QGLTDTIDSHIDNKMSESDKFETTIPENIGEHYNVDTGVLSNREGSDGGNEVIAKVTENN 484

Query: 183 TREDRSE 190
            RE+  E
Sbjct: 485 IRENHLE 491

[Close]
BLAST of PI0027146 vs. ExPASy TrEMBL
Match: A0A5A7VI50 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17G00820 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.6e-13
Identity = 47/67 (70.15%), Postives = 52/67 (77.61%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           QGMTDTIDSHID K SESDRSET  P NI    SV+TG + DREGSDGG +VI +VTE+N
Sbjct: 226 QGMTDTIDSHIDSKQSESDRSETTFPENI---YSVDTGAVLDREGSDGGNKVIEKVTENN 285

Query: 183 TREDRSE 190
            REDR E
Sbjct: 286 IREDRLE 289

[Close]
BLAST of PI0027146 vs. ExPASy TrEMBL
Match: A0A5D3C7K7 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00090 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.3e-09
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           + MTD+IDSHID KM+ESD+SET IP NI EQ +V T VI D +GS+   +V ARV ++ 
Sbjct: 101 ESMTDSIDSHIDSKMNESDKSETIIPENIDEQGNVNTEVILDGKGSNDENKVSARVMQNE 160

Query: 183 TREDRSE 190
            REDRSE
Sbjct: 161 IREDRSE 167

[Close]
BLAST of PI0027146 vs. ExPASy TrEMBL
Match: A0A5A7U7D6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00750 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.9e-09
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           + MTD+IDSHID KMSES++SET IP NI EQ +V T VI + +GS+   EV ARV ++ 
Sbjct: 76  ESMTDSIDSHIDSKMSESEKSETIIPENIDEQGNVNTEVILNGKGSNDENEVSARVMQNE 135

Query: 183 TREDRSE 190
            REDRSE
Sbjct: 136 IREDRSE 142

[Close]
BLAST of PI0027146 vs. ExPASy TrEMBL
Match: A0A5A7UY25 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44334G00040 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 7.4e-08
Identity = 40/69 (57.97%), Postives = 48/69 (69.57%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           QGMTD+IDSHID KMSESDRSETAIP NIGEQ +V+T VI D +GS+   E     T+ +
Sbjct: 197 QGMTDSIDSHIDSKMSESDRSETAIPENIGEQGNVDTEVILDGKGSNDENEGTRSCTKHS 256

Query: 183 TREDRSEKN 192
                S +N
Sbjct: 257 IANYLSYEN 265

[Close]
BLAST of PI0027146 vs. NCBI nr
Match: KAA0067418.1 (tRNA modification GTPase MnmE [Cucumis melo var. makuwa])

HSP 1 Score: 92.8 bits (229), Expect = 3.4e-15
Identity = 48/67 (71.64%), Postives = 57/67 (85.07%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           QG+TDTIDSHID KMSESD+ ET IP NIGE  +V+TGV+S+REGSDGG EVIA+VTE+N
Sbjct: 425 QGLTDTIDSHIDNKMSESDKFETTIPENIGEHYNVDTGVLSNREGSDGGNEVIAKVTENN 484

Query: 183 TREDRSE 190
            RE+  E
Sbjct: 485 IRENHLE 491

[Close]
BLAST of PI0027146 vs. NCBI nr
Match: KAA0065411.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 85.5 bits (210), Expect = 5.5e-13
Identity = 47/67 (70.15%), Postives = 52/67 (77.61%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           QGMTDTIDSHID K SESDRSET  P NI    SV+TG + DREGSDGG +VI +VTE+N
Sbjct: 226 QGMTDTIDSHIDSKQSESDRSETTFPENI---YSVDTGAVLDREGSDGGNKVIEKVTENN 285

Query: 183 TREDRSE 190
            REDR E
Sbjct: 286 IREDRLE 289

[Close]
BLAST of PI0027146 vs. NCBI nr
Match: TYK06339.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 72.4 bits (176), Expect = 4.8e-09
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           + MTD+IDSHID KM+ESD+SET IP NI EQ +V T VI D +GS+   +V ARV ++ 
Sbjct: 101 ESMTDSIDSHIDSKMNESDKSETIIPENIDEQGNVNTEVILDGKGSNDENKVSARVMQNE 160

Query: 183 TREDRSE 190
            REDRSE
Sbjct: 161 IREDRSE 167

[Close]
BLAST of PI0027146 vs. NCBI nr
Match: KAA0050086.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 71.6 bits (174), Expect = 8.2e-09
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           + MTD+IDSHID KMSES++SET IP NI EQ +V T VI + +GS+   EV ARV ++ 
Sbjct: 76  ESMTDSIDSHIDSKMSESEKSETIIPENIDEQGNVNTEVILNGKGSNDENEVSARVMQNE 135

Query: 183 TREDRSE 190
            REDRSE
Sbjct: 136 IREDRSE 142

[Close]
BLAST of PI0027146 vs. NCBI nr
Match: KAA0060782.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-07
Identity = 40/69 (57.97%), Postives = 48/69 (69.57%), Query Frame = 0 

Query: 123 QGMTDTIDSHIDIKMSESDRSETAIPRNIGEQNSVETGVISDREGSDGGAEVIARVTEDN 182
           QGMTD+IDSHID KMSESDRSETAIP NIGEQ +V+T VI D +GS+   E     T+ +
Sbjct: 197 QGMTDSIDSHIDSKMSESDRSETAIPENIGEQGNVDTEVILDGKGSNDENEGTRSCTKHS 256

Query: 183 TREDRSEKN 192
                S +N
Sbjct: 257 IANYLSYEN 265

The following BLAST results are available for this feature:
BLAST of PI0027146 vs.
Match NameE-valueIdentityDescription
BLAST of PI0027146 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7VI581.7e-1571.64tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A5A7VI502.6e-1370.15Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5D3C7K72.3e-0959.70Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16... [more]
A0A5A7U7D63.9e-0959.70Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67... [more]
A0A5A7UY257.4e-0857.97Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44... [more]
BLAST of PI0027146 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0067418.13.4e-1571.64tRNA modification GTPase MnmE [Cucumis melo var. makuwa][more]
KAA0065411.15.5e-1370.15Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
TYK06339.14.8e-0959.70reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0050086.18.2e-0959.70reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0060782.11.5e-0757.97reverse transcriptase [Cucumis melo var. makuwa][more]
BLAST of PI0027146 vs. NCBI nr
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 144..162
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..192
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 178..192

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027146.1PI0027146.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0005525 GTP binding
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups