PI0027124 (gene) Melon (PI 482460) v1

Overview
NamePI0027124
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionReverse transcriptase
Locationchr10: 14039668 .. 14040405 (+)
RNA-Seq ExpressionPI0027124
SyntenyPI0027124
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGCATTCAAGTGGGGTCCTAAGCAACAAAACAGTTTTAACCTACTTAAAACTAAGTTGAGCAACATTCCAGTCCTTAAGCTACCCGACTTCTCCCAAGCATTTGGAGTAGCAGTAGACGCATGTGGTATGGGAGTTGGAGCTGTGTTGTCTCAACAAGGACACCCAATTGAATATTTCAGTGAGAAACTAAGCAAGTCAAGGCAATTATGGAGTACCTATGAGCAAGAACTCTATGCACTGGTAAGGGCTCTAAAGCAGTGGGAACATTACCTTTTATCTAGAGAATTTTTGCTACTAACAGAACATTTCTCATTGAAATACCTACAATCTCAAAAAACAATAAGCAGAATGCGTGCTAGATGGATGTCTTATATCCAAAGATTTGACTTTGTCATTCGACACCAAACCAATAAAGAGAATAAGGTTGCTGATGCATTGAGCAGAAAAGGTAGCCTATTAGCTACTCTATACATAGAGATTATTGTCTTTAACCATCTTCCCGAACTATATGAAAAGGATGGCGATTTTGAGAACATATGGGAGAAGTGTATCAACCATGTCTATGCTGGAGATTTTCATATCCTAGATGGCTTCTTATTCAAAAATGACCTCCTTTGTATACCACAAACTTCCTTAAGAGAAGAGCTAATAAAGGAAGCACACTCCGGAGGACTTGCAGGTCCCTTTTGCCAACAAAAAACATATGAAATCCTTACTAAAAGATATTATTGA

mRNA sequence

ATGGGGGCATTCAAGTGGGGTCCTAAGCAACAAAACAGTTTTAACCTACTTAAAACTAAGTTGAGCAACATTCCAGTCCTTAAGCTACCCGACTTCTCCCAAGCATTTGGAGTAGCAGTAGACGCATGTGGTATGGGAGTTGGAGCTGTGTTGTCTCAACAAGGACACCCAATTGAATATTTCAGTGAGAAACTAAGCAAGTCAAGGCAATTATGGAGTACCTATGAGCAAGAACTCTATGCACTGGTAAGGGCTCTAAAGCAGTGGGAACATTACCTTTTATCTAGAGAATTTTTGCTACTAACAGAACATTTCTCATTGAAATACCTACAATCTCAAAAAACAATAAGCAGAATGCGTGCTAGATGGATGTCTTATATCCAAAGATTTGACTTTGTCATTCGACACCAAACCAATAAAGAGAATAAGGTTGCTGATGCATTGAGCAGAAAAGGTAGCCTATTAGCTACTCTATACATAGAGATTATTGTCTTTAACCATCTTCCCGAACTATATGAAAAGGATGGCGATTTTGAGAACATATGGGAGAAGTGTATCAACCATGTCTATGCTGGAGATTTTCATATCCTAGATGGCTTCTTATTCAAAAATGACCTCCTTTGTATACCACAAACTTCCTTAAGAGAAGAGCTAATAAAGGAAGCACACTCCGGAGGACTTGCAGGTCCCTTTTGCCAACAAAAAACATATGAAATCCTTACTAAAAGATATTATTGA

Coding sequence (CDS)

ATGGGGGCATTCAAGTGGGGTCCTAAGCAACAAAACAGTTTTAACCTACTTAAAACTAAGTTGAGCAACATTCCAGTCCTTAAGCTACCCGACTTCTCCCAAGCATTTGGAGTAGCAGTAGACGCATGTGGTATGGGAGTTGGAGCTGTGTTGTCTCAACAAGGACACCCAATTGAATATTTCAGTGAGAAACTAAGCAAGTCAAGGCAATTATGGAGTACCTATGAGCAAGAACTCTATGCACTGGTAAGGGCTCTAAAGCAGTGGGAACATTACCTTTTATCTAGAGAATTTTTGCTACTAACAGAACATTTCTCATTGAAATACCTACAATCTCAAAAAACAATAAGCAGAATGCGTGCTAGATGGATGTCTTATATCCAAAGATTTGACTTTGTCATTCGACACCAAACCAATAAAGAGAATAAGGTTGCTGATGCATTGAGCAGAAAAGGTAGCCTATTAGCTACTCTATACATAGAGATTATTGTCTTTAACCATCTTCCCGAACTATATGAAAAGGATGGCGATTTTGAGAACATATGGGAGAAGTGTATCAACCATGTCTATGCTGGAGATTTTCATATCCTAGATGGCTTCTTATTCAAAAATGACCTCCTTTGTATACCACAAACTTCCTTAAGAGAAGAGCTAATAAAGGAAGCACACTCCGGAGGACTTGCAGGTCCCTTTTGCCAACAAAAAACATATGAAATCCTTACTAAAAGATATTATTGA

Protein sequence

MGAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYFSEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRARWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENIWEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILTKRYY
Homology
BLAST of PI0027124 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.4e-24
Identity = 61/143 (42.66%), Postives = 87/143 (60.84%), Query Frame = 0

Query: 8   PKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYFSEKLSK 67
           P+  ++F  LK  +S  P+LK+PDF++ F +  DA  + +GAVLSQ GHP+ Y S  L++
Sbjct: 481 PEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDGHPLSYISRTLNE 540

Query: 68  SRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRARWMSYI 127
               +ST E+EL A+V A K + HYLL R F + ++H  L +L   K  +    RW   +
Sbjct: 541 HEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLSWLYRMKDPNSKLTRWRVKL 600

Query: 128 QRFDFVIRHQTNKENKVADALSR 151
             FDF I++   KEN VADALSR
Sbjct: 601 SEFDFDIKYIKGKENCVADALSR 623

BLAST of PI0027124 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.2e-22
Identity = 57/138 (41.30%), Postives = 81/138 (58.70%), Query Frame = 0

Query: 13  SFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYFSEKLSKSRQLW 72
           +F  LK  +   P+L+LPDF + F +  DA  + +GAVLSQ GHPI + S  L+     +
Sbjct: 485 AFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQNGHPISFISRTLNDHELNY 544

Query: 73  STYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRARWMSYIQRFDF 132
           S  E+EL A+V A K + HYLL R+FL+ ++H  L++L + K       RW   +  + F
Sbjct: 545 SAIEKELLAIVWATKTFRHYLLGRQFLIASDHQPLRWLHNLKEPGAKLERWRVRLSEYQF 604

Query: 133 VIRHQTNKENKVADALSR 151
            I +   KEN VADALSR
Sbjct: 605 KIDYIKGKENSVADALSR 622

BLAST of PI0027124 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.2e-19
Identity = 56/156 (35.90%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 4   FKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQG-----HPI 63
           +KW P Q  +   +K  L + PVL+  DFS+   +  DA  + VGAVLSQ+      +P+
Sbjct: 676 WKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYPV 735

Query: 64  EYFSEKLSKSRQLWSTYEQELYALVRALKQWEHYLLS--REFLLLTEHFSL--KYLQSQK 123
            Y+S K+SK++  +S  ++E+ A++++LK W HYL S    F +LT+H +L  +     +
Sbjct: 736 GYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNESE 795

Query: 124 TISRMRARWMSYIQRFDFVIRHQTNKENKVADALSR 151
             ++  ARW  ++Q F+F I ++    N +ADALSR
Sbjct: 796 PENKRLARWQLFLQDFNFEINYRPGSANHIADALSR 831

BLAST of PI0027124 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.2e-19
Identity = 56/156 (35.90%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 4   FKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQG-----HPI 63
           +KW P Q  +   +K  L + PVL+  DFS+   +  DA  + VGAVLSQ+      +P+
Sbjct: 676 WKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYPV 735

Query: 64  EYFSEKLSKSRQLWSTYEQELYALVRALKQWEHYLLS--REFLLLTEHFSL--KYLQSQK 123
            Y+S K+SK++  +S  ++E+ A++++LK W HYL S    F +LT+H +L  +     +
Sbjct: 736 GYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNESE 795

Query: 124 TISRMRARWMSYIQRFDFVIRHQTNKENKVADALSR 151
             ++  ARW  ++Q F+F I ++    N +ADALSR
Sbjct: 796 PENKRLARWQLFLQDFNFEINYRPGSANHIADALSR 831

BLAST of PI0027124 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.2e-19
Identity = 56/156 (35.90%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 4   FKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQG-----HPI 63
           +KW P Q  +   +K  L + PVL+  DFS+   +  DA  + VGAVLSQ+      +P+
Sbjct: 676 WKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYPV 735

Query: 64  EYFSEKLSKSRQLWSTYEQELYALVRALKQWEHYLLS--REFLLLTEHFSL--KYLQSQK 123
            Y+S K+SK++  +S  ++E+ A++++LK W HYL S    F +LT+H +L  +     +
Sbjct: 736 GYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNESE 795

Query: 124 TISRMRARWMSYIQRFDFVIRHQTNKENKVADALSR 151
             ++  ARW  ++Q F+F I ++    N +ADALSR
Sbjct: 796 PENKRLARWQLFLQDFNFEINYRPGSANHIADALSR 831

BLAST of PI0027124 vs. ExPASy TrEMBL
Match: A0A5D3BBL1 (Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00420 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.8e-81
Identity = 151/244 (61.89%), Postives = 190/244 (77.87%), Query Frame = 0

Query: 2   GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
           G F+W         ++  +LSN P   +   +Q F VAV+A     GAVLSQ  HPIEYF
Sbjct: 228 GLFEW--------LVMPVELSNAPSTFMRLMNQPFEVAVNASRTNTGAVLSQSSHPIEYF 287

Query: 62  SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
           SEKLS+ RQ+WSTY+QE+YAL+RALKQWEHYLLS+EFLLLT+HFSLKYLQ+QK+I++M A
Sbjct: 288 SEKLSQLRQMWSTYKQEMYALIRALKQWEHYLLSKEFLLLTDHFSLKYLQAQKSINKMHA 347

Query: 122 RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
           RW+S+++RFDF+I+H++ KENKVA+ALSRK SLL+TL  ++I F HLP LYE D +F  I
Sbjct: 348 RWISFLRRFDFIIKHKSGKENKVANALSRKHSLLSTLSSKLIAFKHLPNLYEDDANFNRI 407

Query: 182 WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
           W K  +H+ AG+FHI+DGFLFK + LCIP TSLRE L+KEAHSGGLAG F Q KT EIL+
Sbjct: 408 WYKRAHHLEAGEFHIVDGFLFKGEKLCIPHTSLREALLKEAHSGGLAGHFGQDKTLEILS 463

Query: 242 KRYY 246
            +YY
Sbjct: 468 SKYY 463

BLAST of PI0027124 vs. ExPASy TrEMBL
Match: A0A5A7SLE0 (Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00090 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.8e-81
Identity = 151/244 (61.89%), Postives = 190/244 (77.87%), Query Frame = 0

Query: 2   GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
           G F+W         ++  +LSN P   +   +Q F VAV+A     GAVLSQ  HPIEYF
Sbjct: 228 GLFEW--------LVMPVELSNAPSTFMRLMNQPFEVAVNASRTNTGAVLSQSSHPIEYF 287

Query: 62  SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
           SEKLS+ RQ+WSTY+QE+YAL+RALKQWEHYLLS+EFLLLT+HFSLKYLQ+QK+I++M A
Sbjct: 288 SEKLSQLRQMWSTYKQEMYALIRALKQWEHYLLSKEFLLLTDHFSLKYLQAQKSINKMHA 347

Query: 122 RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
           RW+S+++RFDF+I+H++ KENKVA+ALSRK SLL+TL  ++I F HLP LYE D +F  I
Sbjct: 348 RWISFLRRFDFIIKHKSGKENKVANALSRKHSLLSTLSSKLIAFKHLPNLYEDDANFNRI 407

Query: 182 WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
           W K  +H+ AG+FHI+DGFLFK + LCIP TSLRE L+KEAHSGGLAG F Q KT EIL+
Sbjct: 408 WYKRAHHLEAGEFHIVDGFLFKGEKLCIPHTSLREALLKEAHSGGLAGHFGQDKTLEILS 463

Query: 242 KRYY 246
            +YY
Sbjct: 468 SKYY 463

BLAST of PI0027124 vs. ExPASy TrEMBL
Match: A0A5D3DGR0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00870 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.6e-76
Identity = 145/183 (79.23%), Postives = 159/183 (86.89%), Query Frame = 0

Query: 2    GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
            GAF+WGPKQQ+SFNLLK  L NI VLKLPDF QAF VAVD CG G+GAVLSQQ HPIEYF
Sbjct: 935  GAFEWGPKQQDSFNLLKESLRNILVLKLPDFFQAFEVAVDTCGTGIGAVLSQQEHPIEYF 994

Query: 62   SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
            SE+LSKSRQ WSTYEQELYALVRALKQWEHYLLSREF+LLT+HFSLKYL SQK+IS M A
Sbjct: 995  SEELSKSRQSWSTYEQELYALVRALKQWEHYLLSREFILLTDHFSLKYLHSQKSISMMHA 1054

Query: 122  RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
            RW+SY QRFDFVI+HQ  KENKVADALSRK +LL  L  EI +FNH+ ELY+KD DFE+I
Sbjct: 1055 RWISYTQRFDFVIKHQAGKENKVADALSRKSNLLFILSSEITIFNHITELYKKDRDFESI 1114

Query: 182  WEK 185
            WEK
Sbjct: 1115 WEK 1117

BLAST of PI0027124 vs. ExPASy TrEMBL
Match: A0A6D2HS27 (Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS4046 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 1.1e-75
Identity = 138/244 (56.56%), Postives = 180/244 (73.77%), Query Frame = 0

Query: 2   GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
           G F WG +Q  SF L+K KL   PVL LPDF + F V  DA G+G+GAVLSQ+  P+ +F
Sbjct: 229 GKFFWGSEQDKSFALIKEKLCTAPVLALPDFDKVFQVECDASGVGIGAVLSQEKRPVAFF 288

Query: 62  SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
           SEKLS++RQ WSTY+QE YA+ RAL+QWEHYL+ REF+L T+H +LK+L SQK I++M A
Sbjct: 289 SEKLSEARQRWSTYDQEFYAVFRALRQWEHYLIQREFILFTDHQALKFLHSQKVINKMHA 348

Query: 122 RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
           RW+S++Q+F F+I+H++   NKVADALSR+ SLL TL  EI+ F  L ELYE D +F+ +
Sbjct: 349 RWVSFLQKFPFIIQHKSGTLNKVADALSRRASLLITLAHEIVGFELLKELYESDAEFKEL 408

Query: 182 WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
           W+KC     + DFHI DGFLFK D LCIP +SLRE+LI++ H GGL+G   + KT   L 
Sbjct: 409 WDKCNGKHPSADFHIRDGFLFKGDRLCIPCSSLREKLIRDLHGGGLSGHLGRDKTIASLE 468

Query: 242 KRYY 246
           +RYY
Sbjct: 469 ERYY 472

BLAST of PI0027124 vs. ExPASy TrEMBL
Match: A0A6D2HLB5 (Reverse transcriptase OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS2198 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 1.1e-75
Identity = 138/244 (56.56%), Postives = 180/244 (73.77%), Query Frame = 0

Query: 2    GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
            G F WG +Q  SF L+K KL   PVL LPDF + F V  DA G+G+GAVLSQ+  P+ +F
Sbjct: 855  GKFFWGSEQDKSFALIKEKLCTAPVLALPDFDKVFQVECDASGVGIGAVLSQEKRPVAFF 914

Query: 62   SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
            SEKLS++RQ WSTY+QE YA+ RAL+QWEHYL+ REF+L T+H +LK+L SQK I++M A
Sbjct: 915  SEKLSEARQRWSTYDQEFYAVFRALRQWEHYLIQREFILFTDHQALKFLHSQKVINKMHA 974

Query: 122  RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
            RW+S++Q+F F+I+H++   NKVADALSR+ SLL TL  EI+ F  L ELYE D +F+ +
Sbjct: 975  RWVSFLQKFPFIIQHKSGTLNKVADALSRRASLLITLAHEIVGFELLKELYESDAEFKEL 1034

Query: 182  WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
            W+KC     + DFHI DGFLFK D LCIP +SLRE+LI++ H GGL+G   + KT   L 
Sbjct: 1035 WDKCNGKHPSADFHIRDGFLFKGDRLCIPCSSLREKLIRDLHGGGLSGHLGRDKTIASLE 1094

Query: 242  KRYY 246
            +RYY
Sbjct: 1095 ERYY 1098

BLAST of PI0027124 vs. NCBI nr
Match: KAE8652794.1 (hypothetical protein Csa_022828 [Cucumis sativus])

HSP 1 Score: 323.9 bits (829), Expect = 1.2e-84
Identity = 153/244 (62.70%), Postives = 189/244 (77.46%), Query Frame = 0

Query: 2   GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
           G+F+WG  +++SF  LK  L++ PVLKLP+F + F V VDA G+G+G VLSQ+GHP+EYF
Sbjct: 140 GSFQWGQVEEDSFCKLKLALASPPVLKLPNFDKPFEVTVDASGLGIGVVLSQEGHPLEYF 199

Query: 62  SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
           SEKLS  RQ WSTYEQELYALVRALKQWEHYLL+  F+LLT+HFSLK+L SQKTISRM A
Sbjct: 200 SEKLSTYRQNWSTYEQELYALVRALKQWEHYLLANTFILLTDHFSLKFLNSQKTISRMHA 259

Query: 122 RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
           RW+ ++QRFDFVI+H + K NKVADALSRKG LL TL  +II F+HL  LY  D DF +I
Sbjct: 260 RWLQFLQRFDFVIKHTSGKSNKVADALSRKGILLTTLQSQIIAFDHLHTLYPTDIDFRSI 319

Query: 182 WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
           WE C NH    D+HI++ FLFK D+LC+P TSLRE +IKE HS GLAG F + KT   + 
Sbjct: 320 WENCSNHKPCKDYHIVNSFLFKGDVLCVPHTSLREAIIKETHSRGLAGHFGRDKTLATII 379

Query: 242 KRYY 246
            +++
Sbjct: 380 SKFF 383

BLAST of PI0027124 vs. NCBI nr
Match: TYJ96334.1 (Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 311.2 bits (796), Expect = 7.8e-81
Identity = 151/244 (61.89%), Postives = 190/244 (77.87%), Query Frame = 0

Query: 2   GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
           G F+W         ++  +LSN P   +   +Q F VAV+A     GAVLSQ  HPIEYF
Sbjct: 228 GLFEW--------LVMPVELSNAPSTFMRLMNQPFEVAVNASRTNTGAVLSQSSHPIEYF 287

Query: 62  SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
           SEKLS+ RQ+WSTY+QE+YAL+RALKQWEHYLLS+EFLLLT+HFSLKYLQ+QK+I++M A
Sbjct: 288 SEKLSQLRQMWSTYKQEMYALIRALKQWEHYLLSKEFLLLTDHFSLKYLQAQKSINKMHA 347

Query: 122 RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
           RW+S+++RFDF+I+H++ KENKVA+ALSRK SLL+TL  ++I F HLP LYE D +F  I
Sbjct: 348 RWISFLRRFDFIIKHKSGKENKVANALSRKHSLLSTLSSKLIAFKHLPNLYEDDANFNRI 407

Query: 182 WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
           W K  +H+ AG+FHI+DGFLFK + LCIP TSLRE L+KEAHSGGLAG F Q KT EIL+
Sbjct: 408 WYKRAHHLEAGEFHIVDGFLFKGEKLCIPHTSLREALLKEAHSGGLAGHFGQDKTLEILS 463

Query: 242 KRYY 246
            +YY
Sbjct: 468 SKYY 463

BLAST of PI0027124 vs. NCBI nr
Match: KAA0026079.1 (Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 311.2 bits (796), Expect = 7.8e-81
Identity = 151/244 (61.89%), Postives = 190/244 (77.87%), Query Frame = 0

Query: 2   GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
           G F+W         ++  +LSN P   +   +Q F VAV+A     GAVLSQ  HPIEYF
Sbjct: 228 GLFEW--------LVMPVELSNAPSTFMRLMNQPFEVAVNASRTNTGAVLSQSSHPIEYF 287

Query: 62  SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
           SEKLS+ RQ+WSTY+QE+YAL+RALKQWEHYLLS+EFLLLT+HFSLKYLQ+QK+I++M A
Sbjct: 288 SEKLSQLRQMWSTYKQEMYALIRALKQWEHYLLSKEFLLLTDHFSLKYLQAQKSINKMHA 347

Query: 122 RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
           RW+S+++RFDF+I+H++ KENKVA+ALSRK SLL+TL  ++I F HLP LYE D +F  I
Sbjct: 348 RWISFLRRFDFIIKHKSGKENKVANALSRKHSLLSTLSSKLIAFKHLPNLYEDDANFNRI 407

Query: 182 WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
           W K  +H+ AG+FHI+DGFLFK + LCIP TSLRE L+KEAHSGGLAG F Q KT EIL+
Sbjct: 408 WYKRAHHLEAGEFHIVDGFLFKGEKLCIPHTSLREALLKEAHSGGLAGHFGQDKTLEILS 463

Query: 242 KRYY 246
            +YY
Sbjct: 468 SKYY 463

BLAST of PI0027124 vs. NCBI nr
Match: KAA0054966.1 (transposon Ty3-I Gag-Pol polyprotein isoform X1 [Cucumis melo var. makuwa] >TYK22755.1 transposon Ty3-I Gag-Pol polyprotein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 295.8 bits (756), Expect = 3.4e-76
Identity = 145/183 (79.23%), Postives = 159/183 (86.89%), Query Frame = 0

Query: 2    GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
            GAF+WGPKQQ+SFNLLK  L NI VLKLPDF QAF VAVD CG G+GAVLSQQ HPIEYF
Sbjct: 935  GAFEWGPKQQDSFNLLKESLRNILVLKLPDFFQAFEVAVDTCGTGIGAVLSQQEHPIEYF 994

Query: 62   SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
            SE+LSKSRQ WSTYEQELYALVRALKQWEHYLLSREF+LLT+HFSLKYL SQK+IS M A
Sbjct: 995  SEELSKSRQSWSTYEQELYALVRALKQWEHYLLSREFILLTDHFSLKYLHSQKSISMMHA 1054

Query: 122  RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
            RW+SY QRFDFVI+HQ  KENKVADALSRK +LL  L  EI +FNH+ ELY+KD DFE+I
Sbjct: 1055 RWISYTQRFDFVIKHQAGKENKVADALSRKSNLLFILSSEITIFNHITELYKKDRDFESI 1114

Query: 182  WEK 185
            WEK
Sbjct: 1115 WEK 1117

BLAST of PI0027124 vs. NCBI nr
Match: KAG7588770.1 (Integrase catalytic core [Arabidopsis suecica])

HSP 1 Score: 295.0 bits (754), Expect = 5.8e-76
Identity = 139/244 (56.97%), Postives = 181/244 (74.18%), Query Frame = 0

Query: 2    GAFKWGPKQQNSFNLLKTKLSNIPVLKLPDFSQAFGVAVDACGMGVGAVLSQQGHPIEYF 61
            G F WGP+Q  SF L+K KL   PVL LPDF + F V  DA G+G+GAVLSQ+  PI +F
Sbjct: 882  GKFHWGPEQDESFALIKEKLCTAPVLALPDFDKIFQVECDASGVGIGAVLSQEKRPIAFF 941

Query: 62   SEKLSKSRQLWSTYEQELYALVRALKQWEHYLLSREFLLLTEHFSLKYLQSQKTISRMRA 121
            SEKLS++RQ WSTY+QE YA+ RAL+QWEHYL+ REF+L T+H +LK+L SQK I++M A
Sbjct: 942  SEKLSEARQKWSTYDQEFYAVFRALRQWEHYLVQREFILFTDHQALKFLHSQKVINKMHA 1001

Query: 122  RWMSYIQRFDFVIRHQTNKENKVADALSRKGSLLATLYIEIIVFNHLPELYEKDGDFENI 181
            RW+S++Q+F F+I+H++   NKVADALSR+ SLL TL  EI+ F  L ELYE D +F+ +
Sbjct: 1002 RWVSFLQKFPFIIQHKSGALNKVADALSRRASLLTTLAHEIVGFEFLKELYETDAEFKEL 1061

Query: 182  WEKCINHVYAGDFHILDGFLFKNDLLCIPQTSLREELIKEAHSGGLAGPFCQQKTYEILT 241
            W+KC     + DFHI +G+LFK D LCIP +SLRE+LI+E H GGL+G   + KT   L 
Sbjct: 1062 WDKCNGKHPSTDFHIREGYLFKGDRLCIPCSSLREKLIRELHGGGLSGHLGRDKTIASLE 1121

Query: 242  KRYY 246
            +RYY
Sbjct: 1122 ERYY 1125

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P043231.4e-2442.66Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
P208252.2e-2241.30Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste... [more]
Q9UR075.2e-1935.90Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT415.2e-1935.90Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT345.2e-1935.90Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
A0A5D3BBL13.8e-8161.89Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5A7SLE03.8e-8161.89Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3DGR01.6e-7679.23Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11... [more]
A0A6D2HS271.1e-7556.56Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS4046 P... [more]
A0A6D2HLB51.1e-7556.56Reverse transcriptase OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS2198 PE=... [more]
Match NameE-valueIdentityDescription
KAE8652794.11.2e-8462.70hypothetical protein Csa_022828 [Cucumis sativus][more]
TYJ96334.17.8e-8161.89Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa][more]
KAA0026079.17.8e-8161.89Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa][more]
KAA0054966.13.4e-7679.23transposon Ty3-I Gag-Pol polyprotein isoform X1 [Cucumis melo var. makuwa] >TYK2... [more]
KAG7588770.15.8e-7656.97Integrase catalytic core [Arabidopsis suecica][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 6..99
e-value: 6.8E-29
score: 99.8
NoneNo IPR availableGENE3D1.10.340.70coord: 182..245
e-value: 3.9E-5
score: 25.8
NoneNo IPR availableGENE3D3.10.20.370coord: 31..100
e-value: 2.8E-7
score: 32.4
NoneNo IPR availablePANTHERPTHR24559:SF360SUBFAMILY NOT NAMEDcoord: 4..169
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 4..169
NoneNo IPR availableCDDcd09274RNase_HI_RT_Ty3coord: 38..151
e-value: 2.8119E-47
score: 151.106
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 4..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027124.1PI0027124.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding