PI0027118 (gene) Melon (PI 482460) v1

Overview
NamePI0027118
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptiontRNA modification GTPase MnmE
Locationchr09: 13098928 .. 13105418 (+)
RNA-Seq ExpressionPI0027118
SyntenyPI0027118
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGCAATCCTCGTCTTTGGCCTAGTTTCGGTCAGAGGCATGGCTCTGCTTCCAGGATTCCGCCATATCATCGCCCATTTTTACAGAACCACACCACCAGTGGCTTTTCTCTTTACCCATTTCTCTACCCCTATTTCTAGACCATCCTCTCTCTATTCCATATCCAAAACCTCCAACCACGTTCTCTCAAAATCCCCGATCAAGTCCCACTCTACTGGGAAAGGTCACCCCCTTATCAATTTGCTCTTATTTTCACTTCAACTTTACTGGACTTCTCCATTTTCTCATTCTTCTTTCTTATTCCAGCAGAGAATACGTTCGTTCTTGCTCCAGATGAGCGCTTGGCTGATTCGCATGCCGGGATTGAGCGCGAACAAATTGAGAATTCAAGCACAATTGCAGCCATTGTCACATCCATTGGGGGTCCTCCTGCTGCTGTGGGGATTGTGCGTTTGTCGGGTCCTCGTGCTGTGAATATCGTTGGGACTTTATTTTGCCCTGCCGCCAAAAAGAAGGGGAAGAATTTGAGCCTGCAACCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTAATTGATGAAGTACAGTAATGTTTACTTTGTCATGTTTGGAAAATTCTCTCAATTCTGTGTCGTTGTTATAGGCTTTTGTTAGTTTGGGATTAATTTGAGCTATGGTTATGTTCTTTTGTGAAGGTTTTGACAGTGCCAATGTTGGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGCTTCAGTGTCACGGGAGCGAAGTATGTCTCCGTCGTGTGCTAAAAGCTTGTTTAGAAGCTGGAGCCCGGCTTGCAGAACCAGGTTTATTTTCTGAACTGGGATTTCTTTCTCATTAAATTCATGGACTATACACAAACCCCCTGCTCATTTATTTACATTAAGTGTTTGCTTACATCCATGGAATTAAAGCAGTGCTAACCCATGGAAAAAAGAAGTGGGGAAAATTTGTTTAGGCTAAGAATTTATGCTTCATTATCATATCGTTACAAAATCTACACTCATGACTGCCTAGGGGAAAGAAACGTTTATAATTAAAAATTTGTATGTATATTTTAGGTGAGTTTACTCTTCGTGCCTTCTTAAATGGACGCTTAGACCTTTCACAAGCAGAAAATGTTGGGAAATTAATTTCAGCCAAGTCTACGGCCGCTGCCGATGCTGCACTGGCTGGAATTCAGGTTGGGTTTTTTTTGTTTTACTGACAATGTTGTCTGAACTGGTGTGTTCATCTAATTTTAGCTACCGAGTCACTGCAACTTGGTGTGGAAGCTACCAATGACTTCTAGTTTTTTTTAAAAATGGAAACGGATCTCTTTATTAATAGTAAAAAAGAGAGACGAGCTCAAAGTACAAGAGGGTTATACAAGAGCTGTCAAAAATACTAGGTATCAGCAGTTCTGCTCGGACATCTCAACTAGGTTGTCACCTCCTTAGCGACCTCATCATATCCAAAAGAACAAAAGCAAAAGAAAAACAAAACTCAATACTAAGCCATGATAGTAGATCTTCACAAAGCCATCATATACAAAATTCAATACTAAGCCATCATATACAAAATTCAATACTAAGCCATCATATCCAATGACTTCTAGTTTACTGAGGCATATCATATTTTGATTGGCAGGGTGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGTATCGAATTACTCACTGAAATTGAGGCCCGTCTAGACTTTGATGATGAGATGCCACCGTTGGATTTGAACATTGTAATGGAAAAAGTACATGCCATGTCACAGGAAGTAGAGACTGCTCTTAAGACAGCCAATTACGACAAGCTTCTTCAATCTGGAATACAGGTAAATTTTAAGAACCGTCTAAATCAGTTTGTTTCCTTGCACAAAAGAGGCATGGATTTGTTAATACCATTTGCCATCTCCTTAAAAACTTGCAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCCAGCCTTCTTAATGCATGGAGCAAAGTAAGCTACTTGACGTTCTCTTATCTCCTTTGAACCTGACCTCCTTTAGATAATTTGATAATTTGTTCTATCTATGAGTCTGACTCTCATAATCTTACTTAAAAATGGGAAGTAAGAAAGTTTAATATTTTCCTCTATATTATTGAACCAAATACAAGGTCTTCTTATATAGAGAAAGACCAACAACCAATAAGGAAAAAATAAGTAAGGAAAAAATTTTTAGACATAAATAAAGAACAAAATTATATTACAAGAGACAAGGAAATTGAACACTTCCCCTCAAGCTGGTTTAACTCAACCCCTGCCATTGTGAATAGGAAAGTTTGGTCTGGAAGTAGGATGCACTGCTGATTTCGACATCTAGGGTTCCGACGGTGGCTATGGTTGTGCAGTTGTTAAAATTACCGATGTATAGCAAAAAATGCAAGAAAACACAGCTGCTGGCGATGTCCAGGAAGTTGTGGTCCGTGGTGGCGATTGGAGAAGGCCTTCTTATATAGAGAAAGACCAACAACCAATAAGGAAAAAATAATTATAGACATAAATAAAGAACAAAATAATGTAAGAGAAAAGGACATTCAACAATCTTCATATCAGTTTTTGCTTGTTCCTTGTTATTTCCATTGAGTCATCGTCTATCCGCATACTCTTAAAAGCACCTAGTGCTTTGTCACTGTAAGAGGGAGAGGGATAATCTTGCATGACTTCCAGATTTGGTGTTATGTGAATTGAAATCTGACAAAATCTTGGTACTGTATTACTACTTTCACACTAGTAATGGTTTTTCTGAAAAGGATGGTGAAACATCTGAATATTCTTCCTTTACATGGAAAGGATTGATTTGAATTTTGAAGGACTGTTGGTTGAGAAGCACAGTGAGGATTTTATAGTAACTCAGCAGAGGAAGGGGCTCTACTTCAACATTTCTTGGAAGATGAACATTGTGTTGTGACTCTTAACTCTTCATGGTCAAGTTTGTTGCAAATATAGGGACAGTCAAACCTATTGTGCAAAATTCTGGGGTGGTGGAGAGGACCGGGCATGAACACATGAGCTAGTAAAGGAGTTGGTAATCTACCTGATGCTATATTGGATTTCAAACTTGCTAATGGCTTTTGTCATTATTCTGTGCAGTGTGCCTATGTTGACATGAAGTTACAGAAATGGATGAAAGTGGGGAGTTGCACGAAAATTTATAGGAACTCATTAAATTTGGGAATCTGGTGTCTTATGTTTTTGTCCTTACCATGAACATGAGTTTTAGAGGGTAGATCTCAAAGAAGCCGCTAAAGACACAGTTTGGAAGTTGGTTGATTTGGCATATAAAATTGAATTTGGTTGATAGGTTGTTTGTAGAAGTCCATCTTGAGCATCAGAGAGATTTCTTACGCTTGTTGGTTGTGGTTTACATTTTTAGCCACTTCTCTGCAGCCTTCACTTCCCTCAAAAATTTAAAAAAGTTTCATCGTTGCTGCTTTGTGGCCATATGTTTAAGAAGCAATCAAGACATTTTCAGGAAAAAAAAGTTCTTTGACTAGATGCTATTAAGTCTCTTTTGTGAGGAAGTGTGGTTAAAGAGAAAGTAGAGAATTTTTTAAGGAGTGTAAAGTGTTAAAATAAATTAGGGTTTATGATCAAATTAATATTTTCCTTTCTAGTATTTTCTTTTTCTGTTATTTTCCTTGTTTGTATTACTGGGAGTCTATTTTTTCAGTAATTTTCCATTATGATTATTCAAGTTATTATTCAATAATATTATAAACATTTAACATGGTATCAATATCATAGCTTTAGGGTTTCTTTCATATTATTCAACTTCTGCCACCTTGATTCTGTCTCCATCGACGGTAGCATCAGTGGAGACGGAGCAAAATCTGCTGCAATGCAATTCAGTGCGGCTAGTTTCATCTTCATTTTTCTAGCTCCACCAGTTTGATCTACTGTTTTGTTGACTTTGTTCTTTGTGTTTTAGTCTGTTCCGTTTTCAGATCAGCAGTTTGTGGGTTTTAAATCTCTCTCTCCAGCGCAAAAGTTCTCTGTGTTTTAGTTCTTGGGTTACGTCTCCAGTCTAGAAGTTCTATGTGTTTTAGTTGTTCATGGATTATGTCTCCAATCCAAAAGTTCTCTTTGTTTTAAATCTTCATGGGTTCCAATTTACGTATATCTCTATTTTAGCTAAGAAGTTCGTGGGTAGACCTTTACGTATATCTCTGTTTCTAGTCTAGAAGTTCGTGAATTCTTGTTTCCAACCTAGAAGTTCTTAGTGTTCTAGTTCTCCATGAGCCTGTGTCCAACCCAGACTTGCTGTGATTTGATTCAGTAGTTTGCTTCAGTTTCTATATTTGAGTCACTTCAGTCCATTGTTCACAAACACATTTGTTTCTACCACCTTGAGTTTGGGGGAGGGTGTTAAAATAAATTACGGTTATGATCAAATTAATATTTTCATTGTTTGTATTATTGAGAGTATTTATTTTGTAATTCTCTATTCTGATTATTGAAGTTATTATTCAATAATATTATACAAAATTAACAGGAAATAGGTTCTCAGGATATTTGGAATGCGATTGCTTTGTGGCCTTGTCTTGGAGTTGATTGTTTAATTCTCTTTCAACGCTATGCTAGACAATAGAAAAGTCAATCCTATGTATATCTAAGAAACATGCAGCACTTCGGCTTTTGTGAGGGTGATTTCCCTCTGTTATATTTTCATTTATTCTGAATTGTTTGTGTGGTAGTCTCAGGAAAAAGAAATTGTGAACTTGTTAAAACGTGTTAAAATTTTCTAATCTCACAACAGAAATTTCTGTTTGATATTAGGTGGTTTTCTTTAGCTACCGTTTCTTTTACTCTTAATCACATTATTCTATTGTCACATTTATTTTTTAGCGTGCAGAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCAAGTGTTACAGTTTCTGGTATTCCTGTAACTCTTCTTGACACAGCTGGAATTAGGGAAACAGATGACATCGTAGAAAAGATTGGTATGATTTACGAATTTCCTAATGTTTAGTGTCTTAATTTAGCATTTCACATGATGCCCAATTATTCCTTTTGAGTTAGGTTGAATTGCATTTACACTGTGTAATTGTAACTTATCCTAGGCCTGATCTACCATCAGACCTATAGTGATTGATTATAATAAGATTTTGTAATGGCTTTTGTTATTTCCCCTTTCGGTCTCAAACTCTGGCCAATTTTAATTTGGAAGAAATTATTTTATGGCATGTTTGATTTTTCTTGAGTTGCAAATTGACCACCCGTTCTCCAACTCCTTGCTCATGTCCTTACTCTGTTCTACAGGTGTCGAGAGATCTGAAGCTGCTGCTCTTGGAGCGGATGTCATTATTATGACAATAAGTGCTCTCGATGGATGGACTGCAGAGGATACCATACTTCTCAATAGGATACTTTCAAAAAAGGTTTGGCAAAGTTCGACCACCTGTTTCCTCCAATGAATTCTTCTTTAATGAGTTACTGGTCAAAATCAAAGCTCCACCACGATAAAGAATATTAAAAATGGTGTTTATTGATTATTATTATTTCTTTTAAACGCTTTGAAAATGCAGAAATCAGATGGATCATCCACTCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTTCAAACATGGATGCAATGAGTATAAATCGTGATTCTTTCAATAAACAAGTTTTTACATGTGCTGTCACTGGACAAGGAATACAGAATCTGGAGATGGCAATATCAGAGCTGGTGGGACTCAACAAAACTCTTGCAAGTGGCCGTAGATGGACAGTAAACCAGGTATAACTAACCTATTTCCCTCTTCCCCCAACCAAACAAGAATCCGGTTCTTTGATGGTAAAACTTGATATGACATCATTTCTTGTCTTAATAAATAGAAATAGTAATGAGCAAATGTAGAACTCATGGATTAGTGTTGTTTTGGTTACATTCAGAGACAATGCGAGCAGCTTCTTAGAACCAAGGAGGCATTTAGAAGATTGAAATCTTCCATTGAAGACGAGTTGCCCCCTGACTTTTGGACAGTGGATTTGAGGGATGCTGTATTAGCTCTTGGGGAAATTTGTGGTGAGGATATCTCTGAAGAGGTTTTGTCCAATATTTTTGGCAAGTTCTGTATTGGTAAGTAGATAGTATCATCACTGTATGTACGATTTCATATTATTTGCATTTCTAACCATGGCTCCACTCAAGTCCTATTCAGGGGACTCATCCCATTTTGGTCCCTTCCAACTTTTTGAGGTTTTTTCTTAGGGCATTATCAAATATAGCAAAATGAACTAAAATATTACGAAATATAACAAAATTTCGTTGATAGACATCGATATCCACCAATAGTGGTCTATCATCAATAGATTATAAAATTTTGCTATATTTTGTTAAA

mRNA sequence

CGGCAATCCTCGTCTTTGGCCTAGTTTCGGTCAGAGGCATGGCTCTGCTTCCAGGATTCCGCCATATCATCGCCCATTTTTACAGAACCACACCACCAGTGGCTTTTCTCTTTACCCATTTCTCTACCCCTATTTCTAGACCATCCTCTCTCTATTCCATATCCAAAACCTCCAACCACGTTCTCTCAAAATCCCCGATCAAGTCCCACTCTACTGGGAAAGAGAATACGTTCGTTCTTGCTCCAGATGAGCGCTTGGCTGATTCGCATGCCGGGATTGAGCGCGAACAAATTGAGAATTCAAGCACAATTGCAGCCATTGTCACATCCATTGGGGGTCCTCCTGCTGCTGTGGGGATTGTGCGTTTGTCGGGTCCTCGTGCTGTGAATATCGTTGGGACTTTATTTTGCCCTGCCGCCAAAAAGAAGGGGAAGAATTTGAGCCTGCAACCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTAATTGATGAAGTTTTGACAGTGCCAATGTTGGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGCTTCAGTGTCACGGGAGCGAAGTATGTCTCCGTCGTGTGCTAAAAGCTTGTTTAGAAGCTGGAGCCCGGCTTGCAGAACCAGGGTGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGTATCGAATTACTCACTGAAATTGAGGCCCGTCTAGACTTTGATGATGAGATGCCACCGTTGGATTTGAACATTGTAATGGAAAAAGTACATGCCATGTCACAGGAAGTAGAGACTGCTCTTAAGACAGCCAATTACGACAAGCTTCTTCAATCTGGAATACAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCCAGCCTTCTTAATGCATGGAGCAAACGTGCAGAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCAAGTGTTACAGTTTCTGGTATTCCTGTAACTCTTCTTGACACAGCTGGAATTAGGGAAACAGATGACATCGTAGAAAAGATTGGTGTCGAGAGATCTGAAGCTGCTGCTCTTGGAGCGGATGTCATTATTATGACAATAAGTGCTCTCGATGGATGGACTGCAGAGGATACCATACTTCTCAATAGGATACTTTCAAAAAAGAAATCAGATGGATCATCCACTCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTTCAAACATGGATGCAATGAGTATAAATCGTGATTCTTTCAATAAACAAGTTTTTACATGTGCTGTCACTGGACAAGGAATACAGAATCTGGAGATGGCAATATCAGAGCTGGTGGGACTCAACAAAACTCTTGCAAGTGGCCGTAGATGGACAGTAAACCAGAGACAATGCGAGCAGCTTCTTAGAACCAAGGAGGCATTTAGAAGATTGAAATCTTCCATTGAAGACGAGTTGCCCCCTGACTTTTGGACAGTGGATTTGAGGGATGCTGTATTAGCTCTTGGGGAAATTTGTGGTGAGGATATCTCTGAAGAGGTTTTGTCCAATATTTTTGGCAAGTTCTGTATTGGTAAGTAGATAGTATCATCACTGTATGTACGATTTCATATTATTTGCATTTCTAACCATGGCTCCACTCAAGTCCTATTCAGGGGACTCATCCCATTTTGGTCCCTTCCAACTTTTTGAGGTTTTTTCTTAGGGCATTATCAAATATAGCAAAATGAACTAAAATATTACGAAATATAACAAAATTTCGTTGATAGACATCGATATCCACCAATAGTGGTCTATCATCAATAGATTATAAAATTTTGCTATATTTTGTTAAA

Coding sequence (CDS)

ATGTTGGGAAGTCCAGCCTTCTTAATGCATGGAGCAAACGTGCAGAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCAAGTGTTACAGTTTCTGGTATTCCTGTAACTCTTCTTGACACAGCTGGAATTAGGGAAACAGATGACATCGTAGAAAAGATTGGTGTCGAGAGATCTGAAGCTGCTGCTCTTGGAGCGGATGTCATTATTATGACAATAAGTGCTCTCGATGGATGGACTGCAGAGGATACCATACTTCTCAATAGGATACTTTCAAAAAAGAAATCAGATGGATCATCCACTCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTTCAAACATGGATGCAATGAGTATAAATCGTGATTCTTTCAATAAACAAGTTTTTACATGTGCTGTCACTGGACAAGGAATACAGAATCTGGAGATGGCAATATCAGAGCTGGTGGGACTCAACAAAACTCTTGCAAGTGGCCGTAGATGGACAGTAAACCAGAGACAATGCGAGCAGCTTCTTAGAACCAAGGAGGCATTTAGAAGATTGAAATCTTCCATTGAAGACGAGTTGCCCCCTGACTTTTGGACAGTGGATTTGAGGGATGCTGTATTAGCTCTTGGGGAAATTTGTGGTGAGGATATCTCTGAAGAGGTTTTGTCCAATATTTTTGGCAAGTTCTGTATTGGTAAGTAG

Protein sequence

MLGSPAFLMHGANVQSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDSFNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK
Homology
BLAST of PI0027118 vs. ExPASy Swiss-Prot
Match: Q8KPU2 (tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=mnmE PE=3 SV=3)

HSP 1 Score: 196.1 bits (497), Expect = 4.7e-49
Identity = 105/224 (46.88%), Postives = 146/224 (65.18%), Query Frame = 0

Query: 17  ERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVI 76
           +RAIVT++ GTTRD++E+ + V GIPV +LDTAGIRET D VE+IGVERS  AA  AD++
Sbjct: 247 DRAIVTDLPGTTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGVERSRRAAQSADLV 306

Query: 77  IMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDSFN 136
           ++TI A  GW+AED  +   +        S  PILLVINK D    +   A+++ +  F 
Sbjct: 307 LLTIDASAGWSAEDQTIWEAV--------SDRPILLVINKRDRLSEAERHAIALPQQEFK 366

Query: 137 KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKSSI 196
             V+T A   QGI++LE AI   VG     ++   W +NQRQ   L   + A RR++ ++
Sbjct: 367 AIVWTAAAQQQGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAALTTAQTALRRVEETL 426

Query: 197 EDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           + +LP DFWT+DLR+A+ ALG I GE+I+E +L  IF +FCIGK
Sbjct: 427 QAQLPLDFWTIDLREAIAALGSITGEEIAESMLDLIFSRFCIGK 462

BLAST of PI0027118 vs. ExPASy Swiss-Prot
Match: B0CBB0 (tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=mnmE PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-47
Identity = 102/224 (45.54%), Postives = 140/224 (62.50%), Query Frame = 0

Query: 17  ERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVI 76
           +RAIVT++ GTTRDV+E+ + V GIP+ +LDTAGIRET+D VE+IGV RS  AA  AD++
Sbjct: 244 DRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVTRSHQAAQSADLV 303

Query: 77  IMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDSFN 136
           ++TI A  GWT++D  L               P++L++NK+D  P   +    +  ++  
Sbjct: 304 LLTIDASVGWTSDDQQLYQAF--------QDLPLILIVNKVDLVPQEQV----VYPEAIA 363

Query: 137 KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKSSI 196
           + V T A   QGI  LE AI E V      A+   W +NQRQ   L + + A   ++ +I
Sbjct: 364 QVVSTIAAQNQGISELETAILETVQTQSLKAANLDWAINQRQAAALQKAQAALEHVQGAI 423

Query: 197 EDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
            D+LP DFWT+DLR A+ ALGEI GEDI+E VL  IF +FCIGK
Sbjct: 424 ADQLPLDFWTIDLRGAIQALGEITGEDITESVLDRIFSRFCIGK 455

BLAST of PI0027118 vs. ExPASy Swiss-Prot
Match: Q5N638 (tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=mnmE PE=3 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 1.5e-47
Identity = 103/224 (45.98%), Postives = 144/224 (64.29%), Query Frame = 0

Query: 17  ERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVI 76
           +RAIVT++ G TRD++E+ + V GIPV +LDTAGIRET D VE+IGVERS  AA  AD++
Sbjct: 247 DRAIVTDLPGRTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGVERSRRAAQSADLV 306

Query: 77  IMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDSFN 136
           ++TI A  GW+AED  +   +        S  PILLVINK D    +   A+++ +  F 
Sbjct: 307 LLTIDASAGWSAEDQTIWEAV--------SDRPILLVINKRDRLSEAERHAIALPQQEFK 366

Query: 137 KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKSSI 196
             V+T A   +GI++LE AI   VG     ++   W +NQRQ   L   + A RR++ ++
Sbjct: 367 AIVWTAAAQQKGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAALTTAQTALRRVEETL 426

Query: 197 EDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           + +LP DFWT+DLR+A+ ALG I GE I+E +L  IF +FCIGK
Sbjct: 427 QAQLPLDFWTIDLREAIAALGSITGEGIAESMLDLIFSRFCIGK 462

BLAST of PI0027118 vs. ExPASy Swiss-Prot
Match: Q8YN91 (tRNA modification GTPase MnmE OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=mnmE PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.0e-47
Identity = 104/226 (46.02%), Postives = 146/226 (64.60%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           QS+RAIVT++ GTTRDV+E+ + V GIPV +LDTAGIRET D VEKIGVERS  AA  AD
Sbjct: 243 QSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTAD 302

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           ++++TI A  GWT  D  +  ++  +        P++LV+NKID      + ++    ++
Sbjct: 303 LVLLTIDAATGWTTGDQEIYEQVKHR--------PLILVMNKIDLVEKQLITSLEY-PEN 362

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
             + V T A   QGI +LE AI E+V   K  A+     +NQRQ   L + K +  ++++
Sbjct: 363 ITQIVHTAAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAALTQAKMSLEQVQA 422

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           +I  +LP DFWT+DLR A+ ALGEI GE+++E VL  IF +FCIGK
Sbjct: 423 TITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK 459

BLAST of PI0027118 vs. ExPASy Swiss-Prot
Match: Q3MBM5 (tRNA modification GTPase MnmE OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=mnmE PE=3 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.7e-47
Identity = 104/226 (46.02%), Postives = 145/226 (64.16%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           QS+RAIVT++ GTTRDV+E+ + V GIPV +LDTAGIRET D VEKIGVERS  AA  AD
Sbjct: 247 QSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVERSRQAANTAD 306

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           ++++TI A  GWT  D  +  ++  +        P++LV+NKID      + ++   + +
Sbjct: 307 LVLLTIDAATGWTTGDQEIYEQVKHR--------PLILVMNKIDLVDKKLITSLEYPK-N 366

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
             + V T A   QGI  LE AI E+V   K  A+     +NQRQ   L + K +  ++++
Sbjct: 367 ITQIVHTAAAQKQGIDALETAILEIVQTGKVKAADMDLAINQRQAAALTQAKISLEQVQA 426

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           +I  +LP DFWT+DLR A+ ALGEI GE+++E VL  IF +FCIGK
Sbjct: 427 TITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRIFSRFCIGK 463

BLAST of PI0027118 vs. ExPASy TrEMBL
Match: A0A0A0KTK4 (TrmE-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G396040 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.2e-111
Identity = 215/226 (95.13%), Postives = 219/226 (96.90%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD
Sbjct: 336 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 395

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           VIIM ISALDGWTAEDTILLNRILSKKKSD S TPILLVINKIDCAPS  MDAMSINRDS
Sbjct: 396 VIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKIDCAPSPKMDAMSINRDS 455

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
           F+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQC QLLRTKEAF RLKS
Sbjct: 456 FSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKS 515

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SIEDELPPDFWTVDLRDAVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 516 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFCIGK 561

BLAST of PI0027118 vs. ExPASy TrEMBL
Match: A0A5A7VI58 (tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold40G00780 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.8e-110
Identity = 213/227 (93.83%), Postives = 217/227 (95.59%), Query Frame = 0

Query: 14  VQSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGA 73
           VQSERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAA GA
Sbjct: 557 VQSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGA 616

Query: 74  DVIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRD 133
           DVIIM ISA DGWT EDTILLNRILSKKKSD S  PILLV+NKIDCAPS NMDAMSINRD
Sbjct: 617 DVIIMAISAPDGWTEEDTILLNRILSKKKSDESCIPILLVVNKIDCAPSPNMDAMSINRD 676

Query: 134 SFNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLK 193
           SF+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAF RLK
Sbjct: 677 SFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLK 736

Query: 194 SSIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SSIEDELPPDFWTVDLR AVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 737 SSIEDELPPDFWTVDLRAAVLALGEICGEDISEEILSNIFGKFCIGK 783

BLAST of PI0027118 vs. ExPASy TrEMBL
Match: A0A5D3DT93 (tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001540 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.2e-109
Identity = 211/226 (93.36%), Postives = 216/226 (95.58%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAA GAD
Sbjct: 336 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGAD 395

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           VIIM ISA DGWT EDTILLNRILSKKKSD S  PILLV+NKIDCAPS NMDAMSINRDS
Sbjct: 396 VIIMAISAPDGWTEEDTILLNRILSKKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDS 455

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
           F+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAF RLKS
Sbjct: 456 FSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKS 515

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SIEDELPPDFWTVDLR AVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 516 SIEDELPPDFWTVDLRAAVLALGEICGEDISEEILSNIFGKFCIGK 561

BLAST of PI0027118 vs. ExPASy TrEMBL
Match: A0A1S3CCP4 (tRNA modification GTPase MnmE OS=Cucumis melo OX=3656 GN=LOC103499287 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.2e-109
Identity = 211/226 (93.36%), Postives = 216/226 (95.58%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAA GAD
Sbjct: 336 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGAD 395

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           VIIM ISA DGWT EDTILLNRILSKKKSD S  PILLV+NKIDCAPS NMDAMSINRDS
Sbjct: 396 VIIMAISAPDGWTEEDTILLNRILSKKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDS 455

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
           F+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAF RLKS
Sbjct: 456 FSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKS 515

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SIEDELPPDFWTVDLR AVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 516 SIEDELPPDFWTVDLRAAVLALGEICGEDISEEILSNIFGKFCIGK 561

BLAST of PI0027118 vs. ExPASy TrEMBL
Match: A0A6J1JUP0 (uncharacterized protein LOC111489063 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489063 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 6.3e-105
Identity = 206/227 (90.75%), Postives = 218/227 (96.04%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD
Sbjct: 337 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 396

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPS-SNMDAMSINRD 134
           VIIMTISALDGWT EDTILL+ IL+KKKSDGS+TPILLVINKIDCAPS +NMDA+SINRD
Sbjct: 397 VIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINRD 456

Query: 135 SFNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLK 194
           SF+KQVFTCAVTGQGI++LEMAISEL+GLN TLASGRRWTVNQRQCEQLLRTKEA  RLK
Sbjct: 457 SFSKQVFTCAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLK 516

Query: 195 SSIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SSIE+ELP DFWTVDLR AVLALGEI GEDISEEVLSNIFGKFCIGK
Sbjct: 517 SSIEEELPLDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCIGK 563

BLAST of PI0027118 vs. NCBI nr
Match: XP_004140317.1 (uncharacterized protein LOC101217353 isoform X1 [Cucumis sativus] >KGN51006.1 hypothetical protein Csa_007730 [Cucumis sativus])

HSP 1 Score: 412.5 bits (1059), Expect = 2.4e-111
Identity = 215/226 (95.13%), Postives = 219/226 (96.90%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD
Sbjct: 336 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 395

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           VIIM ISALDGWTAEDTILLNRILSKKKSD S TPILLVINKIDCAPS  MDAMSINRDS
Sbjct: 396 VIIMAISALDGWTAEDTILLNRILSKKKSDESCTPILLVINKIDCAPSPKMDAMSINRDS 455

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
           F+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQC QLLRTKEAF RLKS
Sbjct: 456 FSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKS 515

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SIEDELPPDFWTVDLRDAVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 516 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEILSNIFGKFCIGK 561

BLAST of PI0027118 vs. NCBI nr
Match: XP_038874821.1 (tRNA modification GTPase MnmE [Benincasa hispida])

HSP 1 Score: 411.0 bits (1055), Expect = 7.1e-111
Identity = 214/226 (94.69%), Postives = 219/226 (96.90%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD
Sbjct: 336 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 395

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPS NMDA+SIN DS
Sbjct: 396 VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSPNMDAISINHDS 455

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
           F+KQVFTCAVTGQGI+NLEM ISELVGLNKTLASGRRWTVNQRQCEQLLRTKEA  RLKS
Sbjct: 456 FSKQVFTCAVTGQGIENLEMTISELVGLNKTLASGRRWTVNQRQCEQLLRTKEALTRLKS 515

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SIEDELP DFWTVDLRDA LALGEICGEDISEEVLSNIFGKFCIGK
Sbjct: 516 SIEDELPLDFWTVDLRDAALALGEICGEDISEEVLSNIFGKFCIGK 561

BLAST of PI0027118 vs. NCBI nr
Match: KAA0067418.1 (tRNA modification GTPase MnmE [Cucumis melo var. makuwa])

HSP 1 Score: 407.5 bits (1046), Expect = 7.8e-110
Identity = 213/227 (93.83%), Postives = 217/227 (95.59%), Query Frame = 0

Query: 14  VQSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGA 73
           VQSERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAA GA
Sbjct: 557 VQSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGA 616

Query: 74  DVIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRD 133
           DVIIM ISA DGWT EDTILLNRILSKKKSD S  PILLV+NKIDCAPS NMDAMSINRD
Sbjct: 617 DVIIMAISAPDGWTEEDTILLNRILSKKKSDESCIPILLVVNKIDCAPSPNMDAMSINRD 676

Query: 134 SFNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLK 193
           SF+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAF RLK
Sbjct: 677 SFSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLK 736

Query: 194 SSIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SSIEDELPPDFWTVDLR AVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 737 SSIEDELPPDFWTVDLRAAVLALGEICGEDISEEILSNIFGKFCIGK 783

BLAST of PI0027118 vs. NCBI nr
Match: XP_008460482.1 (PREDICTED: tRNA modification GTPase MnmE [Cucumis melo])

HSP 1 Score: 404.4 bits (1038), Expect = 6.6e-109
Identity = 211/226 (93.36%), Postives = 216/226 (95.58%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAA GAD
Sbjct: 336 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGAD 395

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           VIIM ISA DGWT EDTILLNRILSKKKSD S  PILLV+NKIDCAPS NMDAMSINRDS
Sbjct: 396 VIIMAISAPDGWTEEDTILLNRILSKKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDS 455

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
           F+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAF RLKS
Sbjct: 456 FSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKS 515

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SIEDELPPDFWTVDLR AVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 516 SIEDELPPDFWTVDLRAAVLALGEICGEDISEEILSNIFGKFCIGK 561

BLAST of PI0027118 vs. NCBI nr
Match: TYK26555.1 (tRNA modification GTPase MnmE [Cucumis melo var. makuwa])

HSP 1 Score: 404.4 bits (1038), Expect = 6.6e-109
Identity = 211/226 (93.36%), Postives = 216/226 (95.58%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTEIAGTTRDVIEA+VTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAA GAD
Sbjct: 336 KSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGAD 395

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINRDS 134
           VIIM ISA DGWT EDTILLNRILSKKKSD S  PILLV+NKIDCAPS NMDAMSINRDS
Sbjct: 396 VIIMAISAPDGWTEEDTILLNRILSKKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDS 455

Query: 135 FNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRRLKS 194
           F+KQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAF RLKS
Sbjct: 456 FSKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKS 515

Query: 195 SIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           SIEDELPPDFWTVDLR AVLALGEICGEDISEE+LSNIFGKFCIGK
Sbjct: 516 SIEDELPPDFWTVDLRAAVLALGEICGEDISEEILSNIFGKFCIGK 561

BLAST of PI0027118 vs. TAIR 10
Match: AT1G78010.1 (tRNA modification GTPase, putative )

HSP 1 Score: 284.3 bits (726), Expect = 9.3e-77
Identity = 147/229 (64.19%), Postives = 183/229 (79.91%), Query Frame = 0

Query: 15  QSERAIVTEIAGTTRDVIEASVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGAD 74
           +SERAIVTE+AGTTRDV+EA+VTV G+P+TLLDTAGIRET+DIVEKIGVERSE AA  AD
Sbjct: 339 KSERAIVTEVAGTTRDVVEANVTVRGVPITLLDTAGIRETNDIVEKIGVERSETAAKVAD 398

Query: 75  VIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSNMDAMSINR-- 134
           VIIM +SA++GWT EDT LL +I S K       P++LV+NKIDCAP  + D +   R  
Sbjct: 399 VIIMAVSAVEGWTEEDTELLRKIQSDK-------PMILVMNKIDCAPPGSCDQLEDQRKK 458

Query: 135 -DSFNKQVFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFRR 194
            + F+K VFT AVTGQGI+ LE AI E++GL++    G +WTVNQRQCEQL+RTKEA  R
Sbjct: 459 EEVFHKSVFTSAVTGQGIEELEDAILEILGLDRVPTGGHQWTVNQRQCEQLVRTKEALVR 518

Query: 195 LKSSIEDELPPDFWTVDLRDAVLALGEICGEDISEEVLSNIFGKFCIGK 241
           L+ +IEDE+P DFWT++LR+A L+L +I G+D+SEEVLS+IF KFCIGK
Sbjct: 519 LREAIEDEIPIDFWTIELREAALSLAQISGQDVSEEVLSSIFAKFCIGK 560

BLAST of PI0027118 vs. TAIR 10
Match: AT3G12080.1 (GTP-binding family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-09
Identity = 54/195 (27.69%), Postives = 92/195 (47.18%), Query Frame = 0

Query: 12  ANVQSERAIVTEIAGTTRDVIEASVT-VSGIPVTLLDTAGIRETDDI------VEKIGVE 71
           A V+ +R IV+ ++GTTRD I+A  T   G    L+DTAGIR+   +       E + V 
Sbjct: 387 ALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKKSSVASSGSTTEAMSVN 446

Query: 72  RSEAAALGADVIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSN 131
           R+  A   +DV+ + I A+   T +D  +  RI  + K        L+V+NK D  P+ N
Sbjct: 447 RAFRAIRRSDVVALVIEAMACITEQDLKIAERIEREGKG------CLVVVNKWDTIPNKN 506

Query: 132 MDAMSINRDSFNKQ---------VFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVN 191
            +  +   D   ++         V++ A+TG  + N+ +A +       T+   R   ++
Sbjct: 507 QETAAHYEDDVREKLRSLKWAPIVYSTAITGHSVDNIVVAAA-------TVQKERSRRLS 566

BLAST of PI0027118 vs. TAIR 10
Match: AT3G12080.2 (GTP-binding family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-09
Identity = 54/195 (27.69%), Postives = 92/195 (47.18%), Query Frame = 0

Query: 12  ANVQSERAIVTEIAGTTRDVIEASVT-VSGIPVTLLDTAGIRETDDI------VEKIGVE 71
           A V+ +R IV+ ++GTTRD I+A  T   G    L+DTAGIR+   +       E + V 
Sbjct: 387 ALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKKSSVASSGSTTEAMSVN 446

Query: 72  RSEAAALGADVIIMTISALDGWTAEDTILLNRILSKKKSDGSSTPILLVINKIDCAPSSN 131
           R+  A   +DV+ + I A+   T +D  +  RI  + K        L+V+NK D  P+ N
Sbjct: 447 RAFRAIRRSDVVALVIEAMACITEQDLKIAERIEREGKG------CLVVVNKWDTIPNKN 506

Query: 132 MDAMSINRDSFNKQ---------VFTCAVTGQGIQNLEMAISELVGLNKTLASGRRWTVN 191
            +  +   D   ++         V++ A+TG  + N+ +A +       T+   R   ++
Sbjct: 507 QETAAHYEDDVREKLRSLKWAPIVYSTAITGHSVDNIVVAAA-------TVQKERSRRLS 566

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8KPU24.7e-4946.88tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942 / FACH... [more]
B0CBB01.2e-4745.54tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) OX=329... [more]
Q5N6381.5e-4745.98tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301... [more]
Q8YN912.0e-4746.02tRNA modification GTPase MnmE OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX ... [more]
Q3MBM55.7e-4746.02tRNA modification GTPase MnmE OS=Trichormus variabilis (strain ATCC 29413 / PCC ... [more]
Match NameE-valueIdentityDescription
A0A0A0KTK41.2e-11195.13TrmE-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G396040... [more]
A0A5A7VI583.8e-11093.83tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A5D3DT933.2e-10993.36tRNA modification GTPase MnmE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CCP43.2e-10993.36tRNA modification GTPase MnmE OS=Cucumis melo OX=3656 GN=LOC103499287 PE=3 SV=1[more]
A0A6J1JUP06.3e-10590.75uncharacterized protein LOC111489063 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_004140317.12.4e-11195.13uncharacterized protein LOC101217353 isoform X1 [Cucumis sativus] >KGN51006.1 hy... [more]
XP_038874821.17.1e-11194.69tRNA modification GTPase MnmE [Benincasa hispida][more]
KAA0067418.17.8e-11093.83tRNA modification GTPase MnmE [Cucumis melo var. makuwa][more]
XP_008460482.16.6e-10993.36PREDICTED: tRNA modification GTPase MnmE [Cucumis melo][more]
TYK26555.16.6e-10993.36tRNA modification GTPase MnmE [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G78010.19.3e-7764.19tRNA modification GTPase, putative [more]
AT3G12080.11.7e-0927.69GTP-binding family protein [more]
AT3G12080.21.7e-0927.69GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 179..199
NoneNo IPR availablePANTHERPTHR42714TRNA MODIFICATION GTPASE GTPBP3coord: 14..240
NoneNo IPR availablePANTHERPTHR42714:SF2TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIALcoord: 14..240
NoneNo IPR availableSUPERFAMILY116878TrmE connector domaincoord: 20..240
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 17..116
e-value: 1.9E-11
score: 44.1
IPR025867MnmE, helical domainPFAMPF12631MnmE_helicalcoord: 117..237
e-value: 1.3E-21
score: 77.9
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 17..151
e-value: 5.6E-14
score: 50.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 11..164
e-value: 1.1E-28
score: 101.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 16..151
IPR031168TrmE-type guanine nucleotide-binding domainCDDcd04164trmEcoord: 14..161
e-value: 1.07959E-48
score: 155.73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027118.1PI0027118.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
biological_process GO:0002098 tRNA wobble uridine modification
cellular_component GO:0005829 cytosol
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding