PI0027100 (gene) Melon (PI 482460) v1

Overview


Name	PI0027100
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Cystatin
Location	chr09: 16791793 .. 16792338 (-)
RNA-Seq Expression	PI0027100
Synteny	PI0027100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAACACATAAATTAGATTCGAGGACATCAATTGGTAGAAGATTGAAGAACCACTATAAATATGGAACCCGTTGCCATGCATTATATATTAAAAAGCAAACATAATCTCTTACAAAGTATATCTATCGAAAGGTATTAGGAATAATGTGTTCCAAGAATATTGTAATACATCCTCAAGTGCCGATTAAGGATATTGATTGTCCCATTATACAAGGATATGCAAGTTTTGCAGTACACAAGCATAATGAAATTACAGGTGAGAAGCTGCTGTTCAAGAAGGCAATAAATGGGTTGGAATCGGGAGAAAGACTTGGTCGAGTAGTTTACACTTTTGTGTTGGAAGCTGAAAACCCGTCTGGAATTAATTGGTGTTATAAAGCTAGAGTGCTTTATAATCAACGGGCAGCGTTACAACTCTTGCAATTTGAAGATGTACTTCACGAGCATATAAAAAATTAAAGTTGGAAGGCTTTCCCTCTACCCTCTGCCTACTGCATAATATACAAACATACATAAATAAAATACAATTATTATGCTTACCTTT

mRNA sequence

AAATAACACATAAATTAGATTCGAGGACATCAATTGGTAGAAGATTGAAGAACCACTATAAATATGGAACCCGTTGCCATGCATTATATATTAAAAAGCAAACATAATCTCTTACAAAGTATATCTATCGAAAGGTATTAGGAATAATGTGTTCCAAGAATATTGTAATACATCCTCAAGTGCCGATTAAGGATATTGATTGTCCCATTATACAAGGATATGCAAGTTTTGCAGTACACAAGCATAATGAAATTACAGGTGAGAAGCTGCTGTTCAAGAAGGCAATAAATGGGTTGGAATCGGGAGAAAGACTTGGTCGAGTAGTTTACACTTTTGTGTTGGAAGCTGAAAACCCGTCTGGAATTAATTGGTGTTATAAAGCTAGAGTGCTTTATAATCAACGGGCAGCGTTACAACTCTTGCAATTTGAAGATGTACTTCACGAGCATATAAAAAATTAAAGTTGGAAGGCTTTCCCTCTACCCTCTGCCTACTGCATAATATACAAACATACATAAATAAAATACAATTATTATGCTTACCTTT

Coding sequence (CDS)

ATGTGTTCCAAGAATATTGTAATACATCCTCAAGTGCCGATTAAGGATATTGATTGTCCCATTATACAAGGATATGCAAGTTTTGCAGTACACAAGCATAATGAAATTACAGGTGAGAAGCTGCTGTTCAAGAAGGCAATAAATGGGTTGGAATCGGGAGAAAGACTTGGTCGAGTAGTTTACACTTTTGTGTTGGAAGCTGAAAACCCGTCTGGAATTAATTGGTGTTATAAAGCTAGAGTGCTTTATAATCAACGGGCAGCGTTACAACTCTTGCAATTTGAAGATGTACTTCACGAGCATATAAAAAATTAA

Protein sequence

MCSKNIVIHPQVPIKDIDCPIIQGYASFAVHKHNEITGEKLLFKKAINGLESGERLGRVVYTFVLEAENPSGINWCYKARVLYNQRAALQLLQFEDVLHEHIKN

Homology

[Close]

BLAST of PI0027100 vs. ExPASy TrEMBL
Match: A0A5D3C5B1 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00040 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.4e-22
Identity = 57/95 (60.00%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 8   IHPQVPIKDIDCPIIQGYASFAVHKHNEI-TGEKLLFKKAINGLESGERLGRVVYTFVLE 67
           + PQVPIKD+D   IQG+  FAV KHN+I TGE L F K INGLE G R  R+VYT VL 
Sbjct: 11  VEPQVPIKDVDSEEIQGFGRFAVRKHNKISTGENLEFVKVINGLEQGRRGFRIVYTLVLS 70

Query: 68  AENPSGINWCYKARVLYNQRAALQLLQFEDVLHEH 102
           A+N  GI W YKA+V +N RAA+ LL+FE VL E+
Sbjct: 71  AKNSCGIIWTYKAKVFFNNRAAVLLLEFEAVLPEN 105

[Close]

BLAST of PI0027100 vs. ExPASy TrEMBL
Match: A0A5A7TFU6 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.8e-20
Identity = 53/101 (52.48%), Postives = 69/101 (68.32%), Query Frame = 0


Query: 1   MCSKNIVIHPQVPIKDIDCPIIQGYASFAVHKHNEITGEKLLFKKAINGLESGERLGRVV 60
           M S+++     VPIKD+D   IQG   FAV +HNE  GE L F K ING E G R  R++
Sbjct: 1   MSSEDLSNLDPVPIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFEQGRRGVRII 60

Query: 61  YTFVLEAENPSGINWCYKARVLYNQRAALQLLQFEDVLHEH 102
           +T VL A+N +GI W YKA+V +NQRAA++LL+FE VL E+
Sbjct: 61  FTIVLSAKNCNGIIWTYKAKVFFNQRAAVRLLEFEAVLPEN 101

[Close]

BLAST of PI0027100 vs. ExPASy TrEMBL
Match: A0A0A0KRZ8 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.8e-20
Identity = 51/94 (54.26%), Postives = 65/94 (69.15%), Query Frame = 0


Query: 8   IHPQVPIKDIDCPIIQGYASFAVHKHNEITGEKLLFKKAINGLESGERLGRVVYTFVLEA 67
           + P VPIKD+D   I+G+  FAV +HNE TGE L F K INGLE G R  R+ +T VL A
Sbjct: 11  VDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSA 70

Query: 68  ENPSGINWCYKARVLYNQRAALQLLQFEDVLHEH 102
           +N  GI W YK +V  NQRA ++LL+FE VL+E+
Sbjct: 71  KNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNEN 104

[Close]

BLAST of PI0027100 vs. ExPASy TrEMBL
Match: A0A0A0KNL2 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-17
Identity = 52/93 (55.91%), Postives = 63/93 (67.74%), Query Frame = 0


Query: 12  VPIKDIDCPIIQGYASFAVHKHNEI-TGEKLLFKKAINGLESGERLGRVVYTFVLEAENP 71
           VPIKD+D   IQG    AV  HNE  TGE L F K INGLE G R  ++VYT VL A+N 
Sbjct: 21  VPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVYTLVLSAKNS 80

Query: 72  SGINWCYKARVLYNQRAALQLLQFEDVLHEHIK 104
            GI W YKA+V++N RAA++L +FE VL E+ K
Sbjct: 81  LGIVWTYKAKVIFNNRAAVRLPEFEAVLPENGK 113

[Close]

BLAST of PI0027100 vs. ExPASy TrEMBL
Match: A0A0A0KNH4 (Cystatin OS=Cucumis sativus OX=3659 GN=Csa_5G485690 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 7.3e-10
Identity = 35/53 (66.04%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 1  MCSKNIVIHPQVPIKDIDCPIIQGYASFAVHKHNEITGEKLLFKKAINGLESG 54
          MCS+N  + P VPIKDID   IQGYA FA  +HNEITG+KL F++AINGLE G
Sbjct: 1  MCSRNFDV-PPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQG 52

[Close]

BLAST of PI0027100 vs. NCBI nr
Match: KAE8648502.1 (hypothetical protein Csa_008487 [Cucumis sativus])

HSP 1 Score: 127.9 bits (320), Expect = 5.2e-26
Identity = 62/104 (59.62%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 1   MCSKNIVIHPQVPIKDIDCPIIQGYASFAVHKHNEITGEKLLFKKAINGLESGERLGRVV 60
           MCS+N  + P VPIKDID   IQGYA FA  +HNEITG+KL F++AINGLE G R G++ 
Sbjct: 1   MCSRNFDV-PPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGRRFGQIC 60

Query: 61  YTFVLEAENPSGINWCYKARVLYNQRAALQLLQFEDVLHEHIKN 105
           +T VLEA N  G  W Y A V YNQRAALQL++F  VL EH+++
Sbjct: 61  FTLVLEAINSGGFLWTYTAIVFYNQRAALQLMEFGPVLPEHLQH 103

[Close]

BLAST of PI0027100 vs. NCBI nr
Match: KAA0040524.1 (Cystatin [Cucumis melo var. makuwa] >TYK05566.1 Cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 112.8 bits (281), Expect = 1.7e-21
Identity = 57/95 (60.00%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 8   IHPQVPIKDIDCPIIQGYASFAVHKHNEI-TGEKLLFKKAINGLESGERLGRVVYTFVLE 67
           + PQVPIKD+D   IQG+  FAV KHN+I TGE L F K INGLE G R  R+VYT VL 
Sbjct: 11  VEPQVPIKDVDSEEIQGFGRFAVRKHNKISTGENLEFVKVINGLEQGRRGFRIVYTLVLS 70

Query: 68  AENPSGINWCYKARVLYNQRAALQLLQFEDVLHEH 102
           A+N  GI W YKA+V +N RAA+ LL+FE VL E+
Sbjct: 71  AKNSCGIIWTYKAKVFFNNRAAVLLLEFEAVLPEN 105

[Close]

BLAST of PI0027100 vs. NCBI nr
Match: KAE8648508.1 (hypothetical protein Csa_009101, partial [Cucumis sativus])

HSP 1 Score: 106.3 bits (264), Expect = 1.6e-19
Identity = 51/94 (54.26%), Postives = 65/94 (69.15%), Query Frame = 0


Query: 8   IHPQVPIKDIDCPIIQGYASFAVHKHNEITGEKLLFKKAINGLESGERLGRVVYTFVLEA 67
           + P VPIKD+D   I+G+  FAV +HNE TGE L F K INGLE G R  R+ +T VL A
Sbjct: 28  VDPAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSA 87

Query: 68  ENPSGINWCYKARVLYNQRAALQLLQFEDVLHEH 102
           +N  GI W YK +V  NQRA ++LL+FE VL+E+
Sbjct: 88  KNSIGIIWTYKVKVFLNQRAGVRLLEFEAVLNEN 121

[Close]

BLAST of PI0027100 vs. NCBI nr
Match: KAA0040521.1 (Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 106.3 bits (264), Expect = 1.6e-19
Identity = 53/101 (52.48%), Postives = 69/101 (68.32%), Query Frame = 0


Query: 1   MCSKNIVIHPQVPIKDIDCPIIQGYASFAVHKHNEITGEKLLFKKAINGLESGERLGRVV 60
           M S+++     VPIKD+D   IQG   FAV +HNE  GE L F K ING E G R  R++
Sbjct: 1   MSSEDLSNLDPVPIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFEQGRRGVRII 60

Query: 61  YTFVLEAENPSGINWCYKARVLYNQRAALQLLQFEDVLHEH 102
           +T VL A+N +GI W YKA+V +NQRAA++LL+FE VL E+
Sbjct: 61  FTIVLSAKNCNGIIWTYKAKVFFNQRAAVRLLEFEAVLPEN 101

[Close]

BLAST of PI0027100 vs. NCBI nr
Match: KGN51193.1 (hypothetical protein Csa_008046 [Cucumis sativus])

HSP 1 Score: 98.6 bits (244), Expect = 3.4e-17
Identity = 52/93 (55.91%), Postives = 63/93 (67.74%), Query Frame = 0


Query: 12  VPIKDIDCPIIQGYASFAVHKHNEI-TGEKLLFKKAINGLESGERLGRVVYTFVLEAENP 71
           VPIKD+D   IQG    AV  HNE  TGE L F K INGLE G R  ++VYT VL A+N 
Sbjct: 21  VPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVYTLVLSAKNS 80

Query: 72  SGINWCYKARVLYNQRAALQLLQFEDVLHEHIK 104
            GI W YKA+V++N RAA++L +FE VL E+ K
Sbjct: 81  LGIVWTYKAKVIFNNRAAVRLPEFEAVLPENGK 113

The following BLAST results are available for this feature:

BLAST of PI0027100 vs.


Match Name	E-value	Identity	Description

BLAST of PI0027100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C5B1	8.4e-22	60.00	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00040 PE=4 S...	[more]
A0A5A7TFU6	7.8e-20	52.48	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 S...	[more]
A0A0A0KRZ8	7.8e-20	54.26	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487690 PE...	[more]
A0A0A0KNL2	1.6e-17	55.91	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G487700 PE...	[more]
A0A0A0KNH4	7.3e-10	66.04	Cystatin OS=Cucumis sativus OX=3659 GN=Csa_5G485690 PE=4 SV=1	[more]

BLAST of PI0027100 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648502.1	5.2e-26	59.62	hypothetical protein Csa_008487 [Cucumis sativus]	[more]
KAA0040524.1	1.7e-21	60.00	Cystatin [Cucumis melo var. makuwa] >TYK05566.1 Cystatin [Cucumis melo var. maku...	[more]
KAE8648508.1	1.6e-19	54.26	hypothetical protein Csa_009101, partial [Cucumis sativus]	[more]
KAA0040521.1	1.6e-19	52.48	Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. maku...	[more]
KGN51193.1	3.4e-17	55.91	hypothetical protein Csa_008046 [Cucumis sativus]	[more]

BLAST of PI0027100 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 14..93 e-value: 4.2E-8 score: 33.4
None	No IPR available	GENE3D	3.10.450.10		coord: 11..103 e-value: 1.4E-7 score: 33.4
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 18..96

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0027100.1	PI0027100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity