PI0027020 (gene) Melon (PI 482460) v1

BLAST of PI0027020 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-09
Identity = 39/111 (35.14%), Postives = 59/111 (53.15%), Query Frame = 0

Query: 3   CNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYLILGVDPL 62
           C+  D++       +K   MD         +      ++D +   I   + Y ILG++  
Sbjct: 50  CDNLDSVSRNSSVSEKLKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYAILGLEKN 109

Query: 63  ADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDKAKRAAFD 114
              + IRK YRKL+L +HPDKNK+ G++ AFK VS+A++CLSD   R  FD
Sbjct: 110 CSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFD 160

BLAST of PI0027020 vs. ExPASy Swiss-Prot
Match: Q9UXR9 (Chaperone protein DnaJ OS=Methanosarcina thermophila OX=2210 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.7e-09
Identity = 34/64 (53.12%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 50  TLNVYLILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDKAKR 109
           T + Y ILG+   A  E I+K YRKLAL  HPD+NK  GA+  FK +SEA++ LSD  KR
Sbjct: 4   TRDYYEILGLSRDATPEDIKKSYRKLALKYHPDRNKEPGAEEKFKEISEAYAVLSDPEKR 63

Query: 110 AAFD 114
           A +D
Sbjct: 64  AQYD 67

BLAST of PI0027020 vs. ExPASy Swiss-Prot
Match: Q7ZXQ8 (DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.3e-09
Identity = 33/73 (45.21%), Postives = 45/73 (61.64%), Query Frame = 0

Query: 41  LDGLPQFIATLNVYLILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAW 100
           +DG+ +       Y +LGV P A EE ++K YRKLAL  HPDKN + GA  AFK +  A+
Sbjct: 91  VDGVQRIKKCKTYYEVLGVSPDAGEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAY 150

Query: 101 SCLSDKAKRAAFD 114
           + LS+  KR  +D
Sbjct: 151 AVLSNPEKRKQYD 163

BLAST of PI0027020 vs. ExPASy Swiss-Prot
Match: Q24133 (DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3)

HSP 1 Score: 65.9 bits (159), Expect = 2.3e-09
Identity = 33/60 (55.00%), Postives = 42/60 (70.00%), Query Frame = 0

Query: 54  YLILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDKAKRAAFD 113
           Y ILG++  A ++ I+K YRKLAL  HPDKNKS  A+  FK ++EA+  LSDK KR  FD
Sbjct: 6   YKILGLERKASDDEIKKAYRKLALKYHPDKNKSPQAEERFKEIAEAYEVLSDKKKRDIFD 65

BLAST of PI0027020 vs. ExPASy Swiss-Prot
Match: Q8TQR1 (Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 5.0e-09
Identity = 33/60 (55.00%), Postives = 41/60 (68.33%), Query Frame = 0

Query: 54  YLILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDKAKRAAFD 113
           Y ILG+   A  E I+K YRKLAL  HPD+NK  GA+  FK +SEA++ LSD  KRA +D
Sbjct: 8   YEILGLPKDASVEDIKKTYRKLALQYHPDRNKDPGAEDKFKEISEAYAVLSDTEKRAQYD 67

BLAST of PI0027020 vs. ExPASy TrEMBL
Match: A0A5D3BHC3 (Meiotically up-regulated gene 184 protein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00500 PE=4 SV=1)

HSP 1 Score: 1293.9 bits (3347), Expect = 0.0e+00
Identity = 680/842 (80.76%), Postives = 702/842 (83.37%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNKDDAIKAKQ+AEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL     
Sbjct: 1   MDCNKDDAIKAKQVAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  IDGCIDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
           AKRA FD+KRNIRGMPMKS EIRS VPIVRNGFHN FPNNNLNRWQWRSD+EV SAPASH
Sbjct: 121 AKRAVFDHKRNIRGMPMKSNEIRSPVPIVRNGFHNLFPNNNLNRWQWRSDNEVPSAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM
Sbjct: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAYNHFKKSFN EKTEFSARG MDSAGYGSMESMHKSFHS TSCK+RA ES QAS
Sbjct: 241 QQQASSAYNHFKKSFNVEKTEFSARGSMDSAGYGSMESMHKSFHSGTSCKVRATESTQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFSFFKSSSPKMKVGHK GISAAMKEEFSP EDH PNKGDAGLASTSFNNS C 
Sbjct: 301 ASSAAKAFSFFKSSSPKMKVGHKDGISAAMKEEFSPREDHIPNKGDAGLASTSFNNSTCS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALK----------- 420
           AHKGDRRKKKMRISGHKMQG+IKNFLRQIEIDNAGIIKESSG +  + +           
Sbjct: 361 AHKGDRRKKKMRISGHKMQGNIKNFLRQIEIDNAGIIKESSGSQKYSFEGRRPITGKFRP 420

Query: 421 -EEGQSLKSLDL--LIQGSF---------------------------------LRGKSVG 480
               + L  ++L  ++ G                                     GKSV 
Sbjct: 421 PNNTRELSQVELRQMLMGKARNEICKKLNEWKADASSTILQRMANSNKNLVEEKEGKSVV 480

Query: 481 LNGMDSDKDLNTACSKDELQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE- 540
           LNGM+S KDLNTACSKDELQTTYTLP  SSE+PDTKDSESFSMSVPDPDF+DFDKDRAE 
Sbjct: 481 LNGMESSKDLNTACSKDELQTTYTLPSNSSESPDTKDSESFSMSVPDPDFYDFDKDRAEK 540

Query: 541 --------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600
                                     ++SLKPFKMRISWLNSKSNIELAPLNWIGCGFPK
Sbjct: 541 SFGSNQVWAVYDDDDGMPRYYAMVHKVLSLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600

Query: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDD 660
           TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD              LTPDD
Sbjct: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGDVWALYRNWSPDWNELTPDD 660

Query: 661 VIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVP 720
           VIHKYDMVEVLEDYNEDKG AVVPLVKVVGFKTVF++HSNPSKIRNIPREEMFRFSHQVP
Sbjct: 661 VIHKYDMVEVLEDYNEDKGAAVVPLVKVVGFKTVFKKHSNPSKIRNIPREEMFRFSHQVP 720

Query: 721 SCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKC 739
           SCLLTGLEGQNAPAGC ELDPAATPLELLQVANE EIELEEAVESAEQAKDRHA+EAIKC
Sbjct: 721 SCLLTGLEGQNAPAGCWELDPAATPLELLQVANEAEIELEEAVESAEQAKDRHAIEAIKC 780

BLAST of PI0027020 vs. ExPASy TrEMBL
Match: A0A1S3CLK6 (uncharacterized protein LOC103502316 OS=Cucumis melo OX=3656 GN=LOC103502316 PE=4 SV=1)

HSP 1 Score: 1293.9 bits (3347), Expect = 0.0e+00
Identity = 680/842 (80.76%), Postives = 702/842 (83.37%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNKDDAIKAKQ+AEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL     
Sbjct: 1   MDCNKDDAIKAKQVAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  IDGCIDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
           AKRA FD+KRNIRGMPMKS EIRS VPIVRNGFHN FPNNNLNRWQWRSD+EV SAPASH
Sbjct: 121 AKRAVFDHKRNIRGMPMKSNEIRSPVPIVRNGFHNLFPNNNLNRWQWRSDNEVPSAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM
Sbjct: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAYNHFKKSFN EKTEFSARG MDSAGYGSMESMHKSFHS TSCK+RA ES QAS
Sbjct: 241 QQQASSAYNHFKKSFNVEKTEFSARGSMDSAGYGSMESMHKSFHSGTSCKVRATESTQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFSFFKSSSPKMKVGHK GISAAMKEEFSP EDH PNKGDAGLASTSFNNS C 
Sbjct: 301 ASSAAKAFSFFKSSSPKMKVGHKDGISAAMKEEFSPREDHIPNKGDAGLASTSFNNSTCS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALK----------- 420
           AHKGDRRKKKMRISGHKMQG+IKNFLRQIEIDNAGIIKESSG +  + +           
Sbjct: 361 AHKGDRRKKKMRISGHKMQGNIKNFLRQIEIDNAGIIKESSGSQKYSFEGRRPITGKFRP 420

Query: 421 -EEGQSLKSLDL--LIQGSF---------------------------------LRGKSVG 480
               + L  ++L  ++ G                                     GKSV 
Sbjct: 421 PNNTRELSQVELRQMLMGKARNEICKKLNEWKADASSTILQRMANSNKNLVEEKEGKSVV 480

Query: 481 LNGMDSDKDLNTACSKDELQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE- 540
           LNGM+S KDLNTACSKDELQTTYTLP  SSE+PDTKDSESFSMSVPDPDF+DFDKDRAE 
Sbjct: 481 LNGMESSKDLNTACSKDELQTTYTLPSNSSESPDTKDSESFSMSVPDPDFYDFDKDRAEK 540

Query: 541 --------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600
                                     ++SLKPFKMRISWLNSKSNIELAPLNWIGCGFPK
Sbjct: 541 SFGSNQVWAVYDDDDGMPRYYAMVHKVLSLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600

Query: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDD 660
           TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD              LTPDD
Sbjct: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGDVWALYRNWSPDWNELTPDD 660

Query: 661 VIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVP 720
           VIHKYDMVEVLEDYNEDKG AVVPLVKVVGFKTVF++HSNPSKIRNIPREEMFRFSHQVP
Sbjct: 661 VIHKYDMVEVLEDYNEDKGAAVVPLVKVVGFKTVFKKHSNPSKIRNIPREEMFRFSHQVP 720

Query: 721 SCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKC 739
           SCLLTGLEGQNAPAGC ELDPAATPLELLQVANE EIELEEAVESAEQAKDRHA+EAIKC
Sbjct: 721 SCLLTGLEGQNAPAGCWELDPAATPLELLQVANEAEIELEEAVESAEQAKDRHAIEAIKC 780

BLAST of PI0027020 vs. ExPASy TrEMBL
Match: A0A5A7URI2 (Meiotically up-regulated gene 184 protein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00510 PE=4 SV=1)

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 678/842 (80.52%), Postives = 700/842 (83.14%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNKDDAIKAKQ+AEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL     
Sbjct: 1   MDCNKDDAIKAKQVAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  IDGCIDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
           AKRA FD+KRNIRGMPMKS EIRS VPIVRNGFHN FPNNNLNRWQWRSD+EV SAP+SH
Sbjct: 121 AKRAVFDHKRNIRGMPMKSNEIRSPVPIVRNGFHNLFPNNNLNRWQWRSDNEVPSAPSSH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM
Sbjct: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAYNHFKKSFN EKTEFSARG MDSAGYGSMESMHKSFHS TSCK+RA ES QAS
Sbjct: 241 QQQASSAYNHFKKSFNVEKTEFSARGSMDSAGYGSMESMHKSFHSGTSCKVRATESTQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFSFFKSSSPKMKVGHK GISAAMKEEFSP EDHTPNKGDAGLASTSFNNS C 
Sbjct: 301 ASSAAKAFSFFKSSSPKMKVGHKDGISAAMKEEFSPREDHTPNKGDAGLASTSFNNSTCS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALK----------- 420
           AHKGDRRKKKMRISGHKMQG+IKNFLRQIEIDNAGIIKESSG +  + +           
Sbjct: 361 AHKGDRRKKKMRISGHKMQGNIKNFLRQIEIDNAGIIKESSGSQKYSFEGRRPITGKFRP 420

Query: 421 -EEGQSLKSLDL--LIQGSF---------------------------------LRGKSVG 480
               + L  ++L  ++ G                                     GKSV 
Sbjct: 421 PNNTRELSQVELRQMLMGKARNEICKKLNEWKADASSTILQRMANSNKNLVEEKEGKSVV 480

Query: 481 LNGMDSDKDLNTACSKDELQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE- 540
           LNGM+S KDLNTACSKDELQTTYTLP  SSE+PDTKDSESFSMSVPDPDF+DFDKDRAE 
Sbjct: 481 LNGMESSKDLNTACSKDELQTTYTLPSNSSESPDTKDSESFSMSVPDPDFYDFDKDRAEK 540

Query: 541 --------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600
                                     +ISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK
Sbjct: 541 SFGSNQVWAVYDDDDGMPRYYAMVHKVISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600

Query: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDD 660
           TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFP KGD              LTPDD
Sbjct: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPGKGDVWALYRNWSPDWNELTPDD 660

Query: 661 VIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVP 720
           VIHKYDMVEVLEDYNEDKG AVVPLVKVVGFKTVF++HSNPSKIRNIPREEMFRFSHQVP
Sbjct: 661 VIHKYDMVEVLEDYNEDKGAAVVPLVKVVGFKTVFKKHSNPSKIRNIPREEMFRFSHQVP 720

Query: 721 SCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKC 739
           SCLLTGLEGQNAPAGC ELDPAATPLELLQV NE EIELEEAVESAEQAKDRHA+EAIKC
Sbjct: 721 SCLLTGLEGQNAPAGCWELDPAATPLELLQVPNEAEIELEEAVESAEQAKDRHAIEAIKC 780

BLAST of PI0027020 vs. ExPASy TrEMBL
Match: A0A0A0LR82 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G001510 PE=4 SV=1)

HSP 1 Score: 1249.2 bits (3231), Expect = 0.0e+00
Identity = 666/843 (79.00%), Postives = 687/843 (81.49%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNK+DAIKAKQ+AE+KFVEMDIATAVRFALRAHSLYP LDGL QFIATLNVYL     
Sbjct: 1   MDCNKEDAIKAKQVAERKFVEMDIATAVRFALRAHSLYPGLDGLSQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  IDGCSDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
           AKRA FD+KRNIRGMPMKS EIRSSVPIVRNGFHN FPNNNLNRW  RSDDEV  APASH
Sbjct: 121 AKRAVFDHKRNIRGMPMKSTEIRSSVPIVRNGFHNLFPNNNLNRWHRRSDDEVLKAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
            VKPTFWT+CNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNG PPSSPWTFNM
Sbjct: 181 LVKPTFWTICNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGKPPSSPWTFNM 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSA+NHFKKSFN EKT+FSARG MDSAGYGSMES HKSFHS TSC LRA ES QAS
Sbjct: 241 QQQASSAFNHFKKSFNVEKTKFSARGSMDSAGYGSMESTHKSFHSGTSCNLRATESTQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFS FKSSS +MKVGHK GISAAMKEEFSPSEDHTPNKGDA LAST FNNSAC 
Sbjct: 301 ASSAAKAFSLFKSSSSEMKVGHKDGISAAMKEEFSPSEDHTPNKGDASLASTPFNNSACS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALKEEGQS------ 420
           AHKGDRRKKKMRISGHKMQG+IKNFLRQIEIDNAGIIKESSG +      EG+S      
Sbjct: 361 AHKGDRRKKKMRISGHKMQGNIKNFLRQIEIDNAGIIKESSGSEKYIF--EGRSPITGKF 420

Query: 421 -------LKSLDL--LIQGSF---------------------------------LRGKSV 480
                  L  ++L  ++ G                                     GKSV
Sbjct: 421 RSANTRELSQVELRQMLMGKARIGIRKKLNEWKADASSTILQRMANSNKNLVEEKEGKSV 480

Query: 481 GLNGMDSDKDLNTACSKDELQTTYTLPS--SENPDTKDSESFSMSVPDPDFHDFDKDRAE 540
            +NGMDS KDLNTACSKDELQTTYTLPS  SE+PDTKDSESFSMSVPDPDFHDFDKDRAE
Sbjct: 481 VINGMDSGKDLNTACSKDELQTTYTLPSNFSESPDTKDSESFSMSVPDPDFHDFDKDRAE 540

Query: 541 ---------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFP 600
                                      +ISLKPFKMRISWLNSKSNIELAPLNWIGCGFP
Sbjct: 541 KSFGSNQVWAVYDDDDGMPRYYAMVHKVISLKPFKMRISWLNSKSNIELAPLNWIGCGFP 600

Query: 601 KTSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPD 660
           KTSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD              LTPD
Sbjct: 601 KTSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGDVWALYRNWSPDWNMLTPD 660

Query: 661 DVIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQV 720
           DVIHKYDMVEVLEDY  DKG AVVPLVKVVGFKTVF+QHSNPSKIRNIPREEMFRFSHQV
Sbjct: 661 DVIHKYDMVEVLEDYGGDKGAAVVPLVKVVGFKTVFKQHSNPSKIRNIPREEMFRFSHQV 720

Query: 721 PSCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIK 739
           PSCLLTGLEGQNAPAGC ELDPAATPLELLQVANE EIELEEAVESAEQAKDRHA+EAIK
Sbjct: 721 PSCLLTGLEGQNAPAGCWELDPAATPLELLQVANEAEIELEEAVESAEQAKDRHAIEAIK 780

BLAST of PI0027020 vs. ExPASy TrEMBL
Match: A0A6J1JAD3 (uncharacterized protein LOC111484988 OS=Cucurbita maxima OX=3661 GN=LOC111484988 PE=4 SV=1)

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 594/841 (70.63%), Postives = 635/841 (75.51%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDC KDDA KAKQIAEKKF EMDIA AV+FALRAHSLYPSLDGLPQFIA LNVYL     
Sbjct: 1   MDCIKDDASKAKQIAEKKFAEMDIAAAVKFALRAHSLYPSLDGLPQFIANLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    +LGVDPLADEETIRKHYR LALILHPDKNKSIGADGAFKIVSEAWS LSDK
Sbjct: 61  IDGCIDWYRVLGVDPLADEETIRKHYRNLALILHPDKNKSIGADGAFKIVSEAWSLLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
            KR AFD KRNIRGM MKS EIRSSVPIVRNGFHN  PNNN NRW WRSDDEV SAPASH
Sbjct: 121 TKREAFDQKRNIRGMAMKSTEIRSSVPIVRNGFHNLSPNNNSNRWHWRSDDEVLSAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVKPTFWT+CNSCKVHFEYLRSYLNHNL+CPNC ISFLAVENP+PPFN NPPSSP TFN 
Sbjct: 181 PVKPTFWTICNSCKVHFEYLRSYLNHNLVCPNCCISFLAVENPAPPFNLNPPSSPRTFNN 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAY+HFKKSF+ EKTEFS R G+D+ GY S  SM KSF S TSCK RAA SM+ S
Sbjct: 241 QQQASSAYSHFKKSFSVEKTEFSPR-GVDAGGYSSTNSMRKSFQSGTSCKHRAAGSMKTS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFSFFK SSPKM +GHK G  AA + E S  EDH P+KGD G ASTS N+SAC 
Sbjct: 301 ASSAAKAFSFFKPSSPKMNMGHKDGKLAAKEVESSLREDHAPDKGDGGFASTSCNDSACS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESS--------GLKSIALKEEG 420
           AHKGDR KKK R +GHKM GSI++FL+Q+EI+N GIIKESS        G +SI  K   
Sbjct: 361 AHKGDRPKKKRRKTGHKMSGSIRDFLKQMEIENGGIIKESSGSQKYSFEGRRSITGKFRS 420

Query: 421 QSLKSLDLL------------------------IQGSFLR--------------GKSVGL 480
            + + L  L                        +  + L+              GKSV  
Sbjct: 421 ANTRELSQLELRQMLMGKARNEIHKKLNEWKADVSSTILQKTENSNKDLFEEKEGKSVVP 480

Query: 481 NGMDSDKDLNTACSKDELQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE-- 540
           NGM S K LNT C KDEL T Y LP  S E PDTK SESFSMSVPDPDFHDFDKDRAE  
Sbjct: 481 NGMKSSKYLNTVCRKDELGTKYPLPPSSGERPDTKASESFSMSVPDPDFHDFDKDRAEKS 540

Query: 541 -------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPKT 600
                                    +ISLKPFKMRISWLNSKSN ELAPLNWIGCGFPKT
Sbjct: 541 FGSNQVWAVYDDDDGMPRYYAMVRKVISLKPFKMRISWLNSKSNTELAPLNWIGCGFPKT 600

Query: 601 SGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDDV 660
           SGDFWIGKHEDYGSLNSFSHKVKQ+KGKRGAIRIFPSKGD              LTPDDV
Sbjct: 601 SGDFWIGKHEDYGSLNSFSHKVKQVKGKRGAIRIFPSKGDVWALYRNWSPDWNELTPDDV 660

Query: 661 IHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVPS 720
           IHKYDMVEVLEDYNED+GVAVVPLVKVVGFKTVFQQH +PSKI+NIPREEMFRFSHQVPS
Sbjct: 661 IHKYDMVEVLEDYNEDRGVAVVPLVKVVGFKTVFQQHIDPSKIQNIPREEMFRFSHQVPS 720

Query: 721 CLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKCA 739
           CLLTGLEGQNAP+GC ELDPAATPLELLQVA E EIELEEA++SAEQA D H LE  K A
Sbjct: 721 CLLTGLEGQNAPSGCWELDPAATPLELLQVAKEAEIELEEAIQSAEQATDGHPLEDTKTA 780

BLAST of PI0027020 vs. NCBI nr
Match: XP_008464425.1 (PREDICTED: uncharacterized protein LOC103502316 [Cucumis melo] >TYJ98449.1 meiotically up-regulated gene 184 protein-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1293.9 bits (3347), Expect = 0.0e+00
Identity = 680/842 (80.76%), Postives = 702/842 (83.37%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNKDDAIKAKQ+AEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL     
Sbjct: 1   MDCNKDDAIKAKQVAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  IDGCIDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
           AKRA FD+KRNIRGMPMKS EIRS VPIVRNGFHN FPNNNLNRWQWRSD+EV SAPASH
Sbjct: 121 AKRAVFDHKRNIRGMPMKSNEIRSPVPIVRNGFHNLFPNNNLNRWQWRSDNEVPSAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM
Sbjct: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAYNHFKKSFN EKTEFSARG MDSAGYGSMESMHKSFHS TSCK+RA ES QAS
Sbjct: 241 QQQASSAYNHFKKSFNVEKTEFSARGSMDSAGYGSMESMHKSFHSGTSCKVRATESTQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFSFFKSSSPKMKVGHK GISAAMKEEFSP EDH PNKGDAGLASTSFNNS C 
Sbjct: 301 ASSAAKAFSFFKSSSPKMKVGHKDGISAAMKEEFSPREDHIPNKGDAGLASTSFNNSTCS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALK----------- 420
           AHKGDRRKKKMRISGHKMQG+IKNFLRQIEIDNAGIIKESSG +  + +           
Sbjct: 361 AHKGDRRKKKMRISGHKMQGNIKNFLRQIEIDNAGIIKESSGSQKYSFEGRRPITGKFRP 420

Query: 421 -EEGQSLKSLDL--LIQGSF---------------------------------LRGKSVG 480
               + L  ++L  ++ G                                     GKSV 
Sbjct: 421 PNNTRELSQVELRQMLMGKARNEICKKLNEWKADASSTILQRMANSNKNLVEEKEGKSVV 480

Query: 481 LNGMDSDKDLNTACSKDELQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE- 540
           LNGM+S KDLNTACSKDELQTTYTLP  SSE+PDTKDSESFSMSVPDPDF+DFDKDRAE 
Sbjct: 481 LNGMESSKDLNTACSKDELQTTYTLPSNSSESPDTKDSESFSMSVPDPDFYDFDKDRAEK 540

Query: 541 --------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600
                                     ++SLKPFKMRISWLNSKSNIELAPLNWIGCGFPK
Sbjct: 541 SFGSNQVWAVYDDDDGMPRYYAMVHKVLSLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600

Query: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDD 660
           TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD              LTPDD
Sbjct: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGDVWALYRNWSPDWNELTPDD 660

Query: 661 VIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVP 720
           VIHKYDMVEVLEDYNEDKG AVVPLVKVVGFKTVF++HSNPSKIRNIPREEMFRFSHQVP
Sbjct: 661 VIHKYDMVEVLEDYNEDKGAAVVPLVKVVGFKTVFKKHSNPSKIRNIPREEMFRFSHQVP 720

Query: 721 SCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKC 739
           SCLLTGLEGQNAPAGC ELDPAATPLELLQVANE EIELEEAVESAEQAKDRHA+EAIKC
Sbjct: 721 SCLLTGLEGQNAPAGCWELDPAATPLELLQVANEAEIELEEAVESAEQAKDRHAIEAIKC 780

BLAST of PI0027020 vs. NCBI nr
Match: KAA0057768.1 (meiotically up-regulated gene 184 protein-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 678/842 (80.52%), Postives = 700/842 (83.14%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNKDDAIKAKQ+AEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL     
Sbjct: 1   MDCNKDDAIKAKQVAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  IDGCIDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
           AKRA FD+KRNIRGMPMKS EIRS VPIVRNGFHN FPNNNLNRWQWRSD+EV SAP+SH
Sbjct: 121 AKRAVFDHKRNIRGMPMKSNEIRSPVPIVRNGFHNLFPNNNLNRWQWRSDNEVPSAPSSH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM
Sbjct: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAYNHFKKSFN EKTEFSARG MDSAGYGSMESMHKSFHS TSCK+RA ES QAS
Sbjct: 241 QQQASSAYNHFKKSFNVEKTEFSARGSMDSAGYGSMESMHKSFHSGTSCKVRATESTQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFSFFKSSSPKMKVGHK GISAAMKEEFSP EDHTPNKGDAGLASTSFNNS C 
Sbjct: 301 ASSAAKAFSFFKSSSPKMKVGHKDGISAAMKEEFSPREDHTPNKGDAGLASTSFNNSTCS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALK----------- 420
           AHKGDRRKKKMRISGHKMQG+IKNFLRQIEIDNAGIIKESSG +  + +           
Sbjct: 361 AHKGDRRKKKMRISGHKMQGNIKNFLRQIEIDNAGIIKESSGSQKYSFEGRRPITGKFRP 420

Query: 421 -EEGQSLKSLDL--LIQGSF---------------------------------LRGKSVG 480
               + L  ++L  ++ G                                     GKSV 
Sbjct: 421 PNNTRELSQVELRQMLMGKARNEICKKLNEWKADASSTILQRMANSNKNLVEEKEGKSVV 480

Query: 481 LNGMDSDKDLNTACSKDELQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE- 540
           LNGM+S KDLNTACSKDELQTTYTLP  SSE+PDTKDSESFSMSVPDPDF+DFDKDRAE 
Sbjct: 481 LNGMESSKDLNTACSKDELQTTYTLPSNSSESPDTKDSESFSMSVPDPDFYDFDKDRAEK 540

Query: 541 --------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600
                                     +ISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK
Sbjct: 541 SFGSNQVWAVYDDDDGMPRYYAMVHKVISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600

Query: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDD 660
           TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFP KGD              LTPDD
Sbjct: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPGKGDVWALYRNWSPDWNELTPDD 660

Query: 661 VIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVP 720
           VIHKYDMVEVLEDYNEDKG AVVPLVKVVGFKTVF++HSNPSKIRNIPREEMFRFSHQVP
Sbjct: 661 VIHKYDMVEVLEDYNEDKGAAVVPLVKVVGFKTVFKKHSNPSKIRNIPREEMFRFSHQVP 720

Query: 721 SCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKC 739
           SCLLTGLEGQNAPAGC ELDPAATPLELLQV NE EIELEEAVESAEQAKDRHA+EAIKC
Sbjct: 721 SCLLTGLEGQNAPAGCWELDPAATPLELLQVPNEAEIELEEAVESAEQAKDRHAIEAIKC 780

BLAST of PI0027020 vs. NCBI nr
Match: XP_004138256.1 (uncharacterized protein LOC101223022 [Cucumis sativus] >XP_031743339.1 uncharacterized protein LOC101223022 [Cucumis sativus] >XP_031743343.1 uncharacterized protein LOC101223022 [Cucumis sativus] >KGN63479.1 hypothetical protein Csa_014081 [Cucumis sativus])

HSP 1 Score: 1249.2 bits (3231), Expect = 0.0e+00
Identity = 666/843 (79.00%), Postives = 687/843 (81.49%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNK+DAIKAKQ+AE+KFVEMDIATAVRFALRAHSLYP LDGL QFIATLNVYL     
Sbjct: 1   MDCNKEDAIKAKQVAERKFVEMDIATAVRFALRAHSLYPGLDGLSQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  IDGCSDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
           AKRA FD+KRNIRGMPMKS EIRSSVPIVRNGFHN FPNNNLNRW  RSDDEV  APASH
Sbjct: 121 AKRAVFDHKRNIRGMPMKSTEIRSSVPIVRNGFHNLFPNNNLNRWHRRSDDEVLKAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
            VKPTFWT+CNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNG PPSSPWTFNM
Sbjct: 181 LVKPTFWTICNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGKPPSSPWTFNM 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSA+NHFKKSFN EKT+FSARG MDSAGYGSMES HKSFHS TSC LRA ES QAS
Sbjct: 241 QQQASSAFNHFKKSFNVEKTKFSARGSMDSAGYGSMESTHKSFHSGTSCNLRATESTQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFS FKSSS +MKVGHK GISAAMKEEFSPSEDHTPNKGDA LAST FNNSAC 
Sbjct: 301 ASSAAKAFSLFKSSSSEMKVGHKDGISAAMKEEFSPSEDHTPNKGDASLASTPFNNSACS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALKEEGQS------ 420
           AHKGDRRKKKMRISGHKMQG+IKNFLRQIEIDNAGIIKESSG +      EG+S      
Sbjct: 361 AHKGDRRKKKMRISGHKMQGNIKNFLRQIEIDNAGIIKESSGSEKYIF--EGRSPITGKF 420

Query: 421 -------LKSLDL--LIQGSF---------------------------------LRGKSV 480
                  L  ++L  ++ G                                     GKSV
Sbjct: 421 RSANTRELSQVELRQMLMGKARIGIRKKLNEWKADASSTILQRMANSNKNLVEEKEGKSV 480

Query: 481 GLNGMDSDKDLNTACSKDELQTTYTLPS--SENPDTKDSESFSMSVPDPDFHDFDKDRAE 540
            +NGMDS KDLNTACSKDELQTTYTLPS  SE+PDTKDSESFSMSVPDPDFHDFDKDRAE
Sbjct: 481 VINGMDSGKDLNTACSKDELQTTYTLPSNFSESPDTKDSESFSMSVPDPDFHDFDKDRAE 540

Query: 541 ---------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFP 600
                                      +ISLKPFKMRISWLNSKSNIELAPLNWIGCGFP
Sbjct: 541 KSFGSNQVWAVYDDDDGMPRYYAMVHKVISLKPFKMRISWLNSKSNIELAPLNWIGCGFP 600

Query: 601 KTSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPD 660
           KTSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD              LTPD
Sbjct: 601 KTSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGDVWALYRNWSPDWNMLTPD 660

Query: 661 DVIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQV 720
           DVIHKYDMVEVLEDY  DKG AVVPLVKVVGFKTVF+QHSNPSKIRNIPREEMFRFSHQV
Sbjct: 661 DVIHKYDMVEVLEDYGGDKGAAVVPLVKVVGFKTVFKQHSNPSKIRNIPREEMFRFSHQV 720

Query: 721 PSCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIK 739
           PSCLLTGLEGQNAPAGC ELDPAATPLELLQVANE EIELEEAVESAEQAKDRHA+EAIK
Sbjct: 721 PSCLLTGLEGQNAPAGCWELDPAATPLELLQVANEAEIELEEAVESAEQAKDRHAIEAIK 780

BLAST of PI0027020 vs. NCBI nr
Match: XP_038878993.1 (uncharacterized protein LOC120071058 [Benincasa hispida] >XP_038878994.1 uncharacterized protein LOC120071058 [Benincasa hispida])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 639/842 (75.89%), Postives = 668/842 (79.33%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDCNKDDAIKAKQ+AEKKFVEMDIA AVRFALRAHSLYP LDGLPQFIATLNVYL     
Sbjct: 1   MDCNKDDAIKAKQVAEKKFVEMDIAAAVRFALRAHSLYPGLDGLPQFIATLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK
Sbjct: 61  FDGCIDWYRILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
            KRAAFD KRN RGMPMKS E RSSVPIVRNGFHN  PNNNLNRW WRSDDEV SAPASH
Sbjct: 121 TKRAAFDQKRNTRGMPMKSTETRSSVPIVRNGFHNLSPNNNLNRWHWRSDDEVLSAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVK TFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNP SSPWTFN 
Sbjct: 181 PVKSTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPSSSPWTFNR 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAY+HFKKSFN EKT+FSARGG+DSAGY SMESMHKSFHS TSCKLRA E MQAS
Sbjct: 241 QQQASSAYSHFKKSFNVEKTQFSARGGVDSAGYSSMESMHKSFHSDTSCKLRATEGMQAS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASS AK FSFFK SSPKMKVGHK GIS AMKEE S  EDH PNKGDAGLA+TSFN+SA  
Sbjct: 301 ASSTAKTFSFFKPSSPKMKVGHKDGISEAMKEEPSLREDHAPNKGDAGLAATSFNDSASS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALK----------- 420
            HKGDR KKK RI+GHKMQG+IKNFLRQ+E+D AGI KESSG +  + +           
Sbjct: 361 VHKGDRPKKKRRITGHKMQGNIKNFLRQMELDGAGITKESSGSQKYSFEGRRTVTGKFRP 420

Query: 421 EEGQSLKSLDL--LIQGSF---------------------------------LRGKSVGL 480
           +  + L  L+L  ++ G                                     GKSV L
Sbjct: 421 DNTRELSQLELRQMLMGKARNEICKKLNEWKADASSTILQRMANSNKDLVEEKEGKSVVL 480

Query: 481 NGMDSDKDLNTACSKDE-LQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE- 540
           NG  S   LN ACSKDE LQT Y  P  S E+PDTKDSESFSMSVPDPDFHDFDKDRAE 
Sbjct: 481 NGTKSSGYLNAACSKDELLQTKYPSPPNSGESPDTKDSESFSMSVPDPDFHDFDKDRAEK 540

Query: 541 --------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600
                                     +ISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK
Sbjct: 541 SFGSNQVWAVYDDDDGMPRYYAMVHKVISLKPFKMRISWLNSKSNIELAPLNWIGCGFPK 600

Query: 601 TSGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDD 660
           TSGDFWIGK+EDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD              LTPDD
Sbjct: 601 TSGDFWIGKYEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGDVWALYRNWSPDWNELTPDD 660

Query: 661 VIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVP 720
           VIHKYDMVEVLEDYNEDKG+AVVPLVKVVG+KTVFQQHS+PSKIRNIPREEMFRFSHQVP
Sbjct: 661 VIHKYDMVEVLEDYNEDKGIAVVPLVKVVGYKTVFQQHSDPSKIRNIPREEMFRFSHQVP 720

Query: 721 SCLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKC 739
           SCLLTGLEGQNAPAGC ELDPAATPLELLQVA +TE ELEEAVESAE+A DRH LEA K 
Sbjct: 721 SCLLTGLEGQNAPAGCWELDPAATPLELLQVAKDTETELEEAVESAEKANDRHPLEATKS 780

BLAST of PI0027020 vs. NCBI nr
Match: XP_022987441.1 (uncharacterized protein LOC111484988 [Cucurbita maxima] >XP_022987442.1 uncharacterized protein LOC111484988 [Cucurbita maxima])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 594/841 (70.63%), Postives = 635/841 (75.51%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           MDC KDDA KAKQIAEKKF EMDIA AV+FALRAHSLYPSLDGLPQFIA LNVYL     
Sbjct: 1   MDCIKDDASKAKQIAEKKFAEMDIAAAVKFALRAHSLYPSLDGLPQFIANLNVYLSAEKR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    +LGVDPLADEETIRKHYR LALILHPDKNKSIGADGAFKIVSEAWS LSDK
Sbjct: 61  IDGCIDWYRVLGVDPLADEETIRKHYRNLALILHPDKNKSIGADGAFKIVSEAWSLLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
            KR AFD KRNIRGM MKS EIRSSVPIVRNGFHN  PNNN NRW WRSDDEV SAPASH
Sbjct: 121 TKREAFDQKRNIRGMAMKSTEIRSSVPIVRNGFHNLSPNNNSNRWHWRSDDEVLSAPASH 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGNPPSSPWTFNM 240
           PVKPTFWT+CNSCKVHFEYLRSYLNHNL+CPNC ISFLAVENP+PPFN NPPSSP TFN 
Sbjct: 181 PVKPTFWTICNSCKVHFEYLRSYLNHNLVCPNCCISFLAVENPAPPFNLNPPSSPRTFNN 240

Query: 241 QQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQAS 300
           QQQASSAY+HFKKSF+ EKTEFS R G+D+ GY S  SM KSF S TSCK RAA SM+ S
Sbjct: 241 QQQASSAYSHFKKSFSVEKTEFSPR-GVDAGGYSSTNSMRKSFQSGTSCKHRAAGSMKTS 300

Query: 301 ASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACF 360
           ASSAAKAFSFFK SSPKM +GHK G  AA + E S  EDH P+KGD G ASTS N+SAC 
Sbjct: 301 ASSAAKAFSFFKPSSPKMNMGHKDGKLAAKEVESSLREDHAPDKGDGGFASTSCNDSACS 360

Query: 361 AHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESS--------GLKSIALKEEG 420
           AHKGDR KKK R +GHKM GSI++FL+Q+EI+N GIIKESS        G +SI  K   
Sbjct: 361 AHKGDRPKKKRRKTGHKMSGSIRDFLKQMEIENGGIIKESSGSQKYSFEGRRSITGKFRS 420

Query: 421 QSLKSLDLL------------------------IQGSFLR--------------GKSVGL 480
            + + L  L                        +  + L+              GKSV  
Sbjct: 421 ANTRELSQLELRQMLMGKARNEIHKKLNEWKADVSSTILQKTENSNKDLFEEKEGKSVVP 480

Query: 481 NGMDSDKDLNTACSKDELQTTYTLP--SSENPDTKDSESFSMSVPDPDFHDFDKDRAE-- 540
           NGM S K LNT C KDEL T Y LP  S E PDTK SESFSMSVPDPDFHDFDKDRAE  
Sbjct: 481 NGMKSSKYLNTVCRKDELGTKYPLPPSSGERPDTKASESFSMSVPDPDFHDFDKDRAEKS 540

Query: 541 -------------------------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPKT 600
                                    +ISLKPFKMRISWLNSKSN ELAPLNWIGCGFPKT
Sbjct: 541 FGSNQVWAVYDDDDGMPRYYAMVRKVISLKPFKMRISWLNSKSNTELAPLNWIGCGFPKT 600

Query: 601 SGDFWIGKHEDYGSLNSFSHKVKQIKGKRGAIRIFPSKGD--------------LTPDDV 660
           SGDFWIGKHEDYGSLNSFSHKVKQ+KGKRGAIRIFPSKGD              LTPDDV
Sbjct: 601 SGDFWIGKHEDYGSLNSFSHKVKQVKGKRGAIRIFPSKGDVWALYRNWSPDWNELTPDDV 660

Query: 661 IHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVPS 720
           IHKYDMVEVLEDYNED+GVAVVPLVKVVGFKTVFQQH +PSKI+NIPREEMFRFSHQVPS
Sbjct: 661 IHKYDMVEVLEDYNEDRGVAVVPLVKVVGFKTVFQQHIDPSKIQNIPREEMFRFSHQVPS 720

Query: 721 CLLTGLEGQNAPAGCCELDPAATPLELLQVANETEIELEEAVESAEQAKDRHALEAIKCA 739
           CLLTGLEGQNAP+GC ELDPAATPLELLQVA E EIELEEA++SAEQA D H LE  K A
Sbjct: 721 CLLTGLEGQNAPSGCWELDPAATPLELLQVAKEAEIELEEAIQSAEQATDGHPLEDTKTA 780

BLAST of PI0027020 vs. TAIR 10
Match: AT2G25560.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 323.6 bits (828), Expect = 4.3e-88
Identity = 243/725 (33.52%), Postives = 351/725 (48.41%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           M+ NK++A +A++IA++KF+  D A A +FAL+A  LYP LDG+ Q +AT +V+L     
Sbjct: 1   MEFNKEEATRAREIAKRKFLANDFAGARKFALKAQFLYPELDGIAQMVATFDVHLSAQNI 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    +LG++P AD+E +RK YRKLA++LHPD+NKS+GA+ AFK +S+AW   SDK
Sbjct: 61  IYGDVDHYGVLGLNPEADDEIVRKRYRKLAVMLHPDRNKSVGAEEAFKFLSQAWGVFSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFH-------NPFPNNNLNRWQWRSDDEV 180
           AKRA +D KRN+ G+  K     SS P   NGF        N     +  R   R+ D  
Sbjct: 121 AKRADYDLKRNV-GL-YKGGGASSSRP-ATNGFQKVTKASGNTTKVKSSKRGIKRASDAS 180

Query: 181 RSAPASHPVKP-----TFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFN 240
            +A  S   +      TFWT+C +C+  +EY   YLN NL+CPNCR  F+AVE   PP +
Sbjct: 181 AAATTSTSAQKTTADGTFWTVCRTCRTQYEYHSVYLNQNLLCPNCRKPFIAVET-DPPGS 240

Query: 241 GNPPSSPWTFNMQQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTS 300
           G   S   TF+  Q     ++  + + +  K     R   ++  YG  +S      + T 
Sbjct: 241 G---SIRKTFHEHQ-----FDSLRHTTDGRKKNVPGRD--NNGVYGEYDSFEWGVFTGTK 300

Query: 301 CKLRAAESMQASASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDA- 360
               A  +         + ++                     K    PS    P +    
Sbjct: 301 TSAHATPTGSRKDEVVRREYT---------------------KRTAGPSSTIPPKRRKVM 360

Query: 361 GLASTSFNNSACFAHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIA 420
             A    N ++C A K    K+   +S  +++  +K   + +      I +    L +I 
Sbjct: 361 ENAVAGGNIASCLAPKSTGVKE---VSEDELKNLLKKKAKSV------ISRNLPELCTIV 420

Query: 421 LKEE--GQSLKSLDLLIQGSFLRGKSVGLNG-----MDSDKDLNTACSKDELQTTYTLPS 480
            + E  G+ +++ DL     F  G SV  N      M++DK+LN+               
Sbjct: 421 AETETNGRGMETEDL---NGFNAGSSVNKNAIESCCMNTDKELNSL-------------- 480

Query: 481 SENPDTKDSESFSMSVPDPDFHDFDKDRAE---------------------------IIS 540
                     + ++ V  PDF DFDKDR E                           +IS
Sbjct: 481 ---------GALTLDVTAPDFCDFDKDRTEKSVKDNQIWAFYDSHEGLPRSYALIHNVIS 540

Query: 541 LKPFKMRISWLNSKSNIELAPLNWIGCGFPKTSGDFWIGKHEDYGSLNSFSHKVKQIKGK 600
           + PFK+R+SWL   +N E +  NW+G G PK+ G F + K   Y S  SFSHKV  +KG 
Sbjct: 541 VDPFKVRMSWLTPVTNGEPSSTNWLGFGIPKSCGGFRVRKTLIYRSPYSFSHKVNLVKGN 600

Query: 601 RGAIRIFPSKGD--------------LTPDDVIHKYDMVEVLEDYNEDKGVAVVPLVKVV 651
            G   I+P  GD              LT  + + +YD+VEV+E Y E+ GV VVPLVKV 
Sbjct: 601 HGEFLIYPRTGDVWALYRKWSPDWNYLTGVETV-EYDIVEVVEGYTEEYGVVVVPLVKVA 654

BLAST of PI0027020 vs. TAIR 10
Match: AT5G53150.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 319.7 bits (818), Expect = 6.2e-87
Identity = 259/777 (33.33%), Postives = 369/777 (47.49%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           M+CNKD+A +A  IAE+K  E D   A +FA +A +L+P LDGL Q    +NVY+     
Sbjct: 1   MECNKDEAKRAMDIAERKMTEKDYTGAKKFANKAQNLFPELDGLKQLFVAINVYISGEKT 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    +LGVDP A +E ++K YRKL L+LHPDKNK  GA+GAF +V+EAW+ LSDK
Sbjct: 61  FAGEADWYGVLGVDPFASDEALKKQYRKLVLMLHPDKNKCKGAEGAFNLVAEAWALLSDK 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLN-RWQWRSDDEVRSAPAS 180
            KR  ++ K   RG  +K+A+ R          H P  N   N R    S    R  PA+
Sbjct: 121 DKRILYNVK---RGKDVKAAQQRFPTTQREIPSHQPTSNGIPNVREHVVSSARARYKPAT 180

Query: 181 ------------------HPVK--PTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLA 240
                             +P +   TFWTMCN C   +EY R YLN  L+CP+C   F+A
Sbjct: 181 RKPAARMDRSRTGSPAFVYPTQESSTFWTMCNKCDTQYEYQRVYLNQTLLCPHCHHGFVA 240

Query: 241 VENPSPPFNGNPPSSPWTFNMQQQASSAYNHFKKSFNAEKTEFSARGGMDSAGYGSMESM 300
            E   P    N P  P   +  Q   S+ N      N+  + +               S+
Sbjct: 241 EEKTPPT---NIPKPPVNISSNQHHRSSKNQASNK-NSNGSSYRRE---------PATSV 300

Query: 301 HKSFHSSTSCKLRAAESMQASASSAAKAFSFFKSSSPKMKVGHKAGISAAMKEEFSPSED 360
           + +F   +S ++  + S  A+  +A    +  +    K            +K  F  +++
Sbjct: 301 NHNFQWDSS-RMGGSYSRNATNETA----NVVQQGQDK------------LKRVFWETQE 360

Query: 361 HTPNKGDAGLASTSFNNSACFAHKGDRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKE 420
               +G        F NS      G+ +++K   S H    S  +    ++      IK+
Sbjct: 361 REAARG--------FTNS----DLGNFKRQKTDDS-HMRGPSAGSRHPYVQALLRSDIKK 420

Query: 421 SSGLKSIALKEEGQS--LKSLDLLIQGSFLRGKSVGLNGMDSDKDLNTACSKDELQTTYT 480
                  AL + GQS   K L ++I  + + GK   +N  + +K+   A S    +   +
Sbjct: 421 -------ALMDRGQSEIFKRLPMMI--AKMEGK---VNPTEGEKNSTKAMSSKASEVERS 480

Query: 481 LPSSENPDTKDS---------ESFSMSVPDPDFHDFDKDRAE------------------ 540
             SS   + + S         E   + VPD DFH+FD DR+E                  
Sbjct: 481 KMSSTANEVERSVEVIPHESDEVKEIVVPDSDFHNFDLDRSESAFKDDQIWAAYDDADGM 540

Query: 541 ---------IISLKPFKMRISWLNSKSNIELAPLNWIGCGFPKTSGDFWIGKHEDYGSLN 600
                    +IS+ PFK++ISWLNSK+  E  P++W+G GF K+ GDF  G++E   +LN
Sbjct: 541 PRFYARIQKVISVNPFKLKISWLNSKTTSEFGPIDWMGAGFAKSCGDFRCGRYESTDTLN 600

Query: 601 SFSHKVKQIKGKRGAIRIFPSKGDL--------------TPDDVIHKYDMVEVLEDYNE- 660
           +FSH V   KG RG + I P KG +              TPD+V HKY+MVEVL+DY E 
Sbjct: 601 AFSHSVDFTKGARGLLHILPKKGQVWALYRNWSPEWDKNTPDEVKHKYEMVEVLDDYTED 660

Query: 661 DKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEMFRFSHQVPSCLLTGLEGQNAPAGC 689
           D+ + V  L+K  GF+ VF++ +    +R I +EEM RFSHQVP  +LTG E  NAP G 
Sbjct: 661 DQSLTVALLLKAEGFRVVFRRCTEKLGVRKIAKEEMLRFSHQVPHYILTGKEADNAPEGF 712

BLAST of PI0027020 vs. TAIR 10
Match: AT2G05250.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 283.5 bits (724), Expect = 4.9e-76
Identity = 234/745 (31.41%), Postives = 356/745 (47.79%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           M+  +++A++ KQIAE++F E D  +A  +AL+A SL+P L+GL Q +AT  VYL     
Sbjct: 1   MEAYREEALRVKQIAERRFAEKDFTSARSYALKAKSLFPDLEGLSQMVATFEVYLASQTR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    +LG+ P A +  ++K Y+K+A++LHPDKNK IGADGAF ++SEAWS LS++
Sbjct: 61  SGGQIDYYAVLGLKPSAGKREVKKQYKKMAVLLHPDKNKCIGADGAFHLISEAWSFLSNE 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
             ++ F YKR      + S E++        G          +R+           P S 
Sbjct: 121 FNKSTFYYKRK---KHIDSTEVQKHSTEYMPGTGTGTGTAVFDRF-----------PPSS 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVE-NPSP---PFNGNPPS-SP 240
               TFWT+C SCKV +EYLR Y+N  L C NCR +F+AVE  P+P   PF+  PPS +P
Sbjct: 181 ERLDTFWTVCTSCKVQYEYLRKYVNKRLSCKNCRGAFIAVETGPAPVSAPFHYTPPSHAP 240

Query: 241 WTFNMQQQASSAYNHFKKSFNA------EKTEFSARGGMDSAGYGSMESMHKSFH-SSTS 300
            +      A  +  +    ++A        T F   G     G+G   S + S+  SS S
Sbjct: 241 PSHAPPSHAPPSNGYGAHGYDAMSRMPTNSTYF--LGHYPGQGHGYDYSTNGSYEWSSYS 300

Query: 301 CKLRAAESMQASASSAAKAFSFFKSSSPKMKVG---HKAGISAAMKEEFSPSEDHTPNKG 360
               +  ++     S+      +K S+  +  G    K G +    ++ +P     PN+ 
Sbjct: 301 GTTTSPGNLDLKRVSSVSNGYPYKHSNSVVSGGINKVKDGSNGTCSKKSTPGGLIYPNQ- 360

Query: 361 DAGLASTSFNNSACFAHKGDRRKKKMRI-SGHKMQGSIKNFLRQIEIDNAGIIKESSGLK 420
                S S + S   A+K  R  KK ++       G ++N LR + +             
Sbjct: 361 ----ISMSAHAS---ANKVGRPGKKSKVFMEAAANGFVENPLRSVSV------------- 420

Query: 421 SIALKEEGQSLKSLDLLIQGSFLRGKSVGLNGMDSDKDLNTACSKDELQTTYTL------ 480
           S     + +  +   L IQ S  R  +  +  +D+ K L      D  Q    +      
Sbjct: 421 SKTANTDAKMDQDYKLHIQSSTRRWSAASV--LDTRKPLIQKARTDIKQRLEMMRLALEA 480

Query: 481 -PSSENPDTKDSESF---------------SMSVPDPDFHDFDKDRA------------- 540
             ++E+    D ++                 ++VPD DFHDFDK+R+             
Sbjct: 481 AAAAEDATPLDEKTVLSCKLGDVTGRKTNGPITVPDSDFHDFDKNRSEESFEPRQIWAIY 540

Query: 541 --------------EIISLKPFKMRISWLNSKSNIELAPLNWIGCGFPKTSGDFWIGKHE 600
                         E++S++PFK+ I++L+SK++IE   + W+  GF K+ G F I   +
Sbjct: 541 DEDDGMPRLYCVVREVLSVQPFKIDIAYLSSKTDIEFGSMKWVQYGFTKSCGHFRIRNSD 600

Query: 601 DYGSLNSFSHKVKQIK-GKRGAIRIFPSKGDL--------------TPDDVIHKYDMVEV 652
               +N FSH +K  K G+ G +RIFP+ G++              TPD+V H+Y+MVE+
Sbjct: 601 IVDHVNIFSHLLKGKKTGRGGCVRIFPTAGEIWAVYKNWSLNWDGSTPDEVRHQYEMVEI 660

BLAST of PI0027020 vs. TAIR 10
Match: AT2G05230.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 283.5 bits (724), Expect = 4.9e-76
Identity = 234/745 (31.41%), Postives = 356/745 (47.79%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNVYL----- 60
           M+  +++A++ KQIAE++F E D  +A  +AL+A SL+P L+GL Q +AT  VYL     
Sbjct: 1   MEAYREEALRVKQIAERRFAEKDFTSARSYALKAKSLFPDLEGLSQMVATFEVYLASQTR 60

Query: 61  ---------ILGVDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDK 120
                    +LG+ P A +  ++K Y+K+A++LHPDKNK IGADGAF ++SEAWS LS++
Sbjct: 61  SGGQIDYYAVLGLKPSAGKREVKKQYKKMAVLLHPDKNKCIGADGAFHLISEAWSFLSNE 120

Query: 121 AKRAAFDYKRNIRGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASH 180
             ++ F YKR      + S E++        G          +R+           P S 
Sbjct: 121 FNKSTFYYKRK---KHIDSTEVQKHSTEYMPGTGTGTGTAVFDRF-----------PPSS 180

Query: 181 PVKPTFWTMCNSCKVHFEYLRSYLNHNLICPNCRISFLAVE-NPSP---PFNGNPPS-SP 240
               TFWT+C SCKV +EYLR Y+N  L C NCR +F+AVE  P+P   PF+  PPS +P
Sbjct: 181 ERLDTFWTVCTSCKVQYEYLRKYVNKRLSCKNCRGAFIAVETGPAPVSAPFHYTPPSHAP 240

Query: 241 WTFNMQQQASSAYNHFKKSFNA------EKTEFSARGGMDSAGYGSMESMHKSFH-SSTS 300
            +      A  +  +    ++A        T F   G     G+G   S + S+  SS S
Sbjct: 241 PSHAPPSHAPPSNGYGAHGYDAMSRMPTNSTYF--LGHYPGQGHGYDYSTNGSYEWSSYS 300

Query: 301 CKLRAAESMQASASSAAKAFSFFKSSSPKMKVG---HKAGISAAMKEEFSPSEDHTPNKG 360
               +  ++     S+      +K S+  +  G    K G +    ++ +P     PN+ 
Sbjct: 301 GTTTSPGNLDLKRVSSVSNGYPYKHSNSVVSGGINKVKDGSNGTCSKKSTPGGLIYPNQ- 360

Query: 361 DAGLASTSFNNSACFAHKGDRRKKKMRI-SGHKMQGSIKNFLRQIEIDNAGIIKESSGLK 420
                S S + S   A+K  R  KK ++       G ++N LR + +             
Sbjct: 361 ----ISMSAHAS---ANKVGRPGKKSKVFMEAAANGFVENPLRSVSV------------- 420

Query: 421 SIALKEEGQSLKSLDLLIQGSFLRGKSVGLNGMDSDKDLNTACSKDELQTTYTL------ 480
           S     + +  +   L IQ S  R  +  +  +D+ K L      D  Q    +      
Sbjct: 421 SKTANTDAKMDQDYKLHIQSSTRRWSAASV--LDTRKPLIQKARTDIKQRLEMMRLALEA 480

Query: 481 -PSSENPDTKDSESF---------------SMSVPDPDFHDFDKDRA------------- 540
             ++E+    D ++                 ++VPD DFHDFDK+R+             
Sbjct: 481 AAAAEDATPLDEKTVLSCKLGDVTGRKTNGPITVPDSDFHDFDKNRSEESFEPRQIWAIY 540

Query: 541 --------------EIISLKPFKMRISWLNSKSNIELAPLNWIGCGFPKTSGDFWIGKHE 600
                         E++S++PFK+ I++L+SK++IE   + W+  GF K+ G F I   +
Sbjct: 541 DEDDGMPRLYCVVREVLSVQPFKIDIAYLSSKTDIEFGSMKWVQYGFTKSCGHFRIRNSD 600

Query: 601 DYGSLNSFSHKVKQIK-GKRGAIRIFPSKGDL--------------TPDDVIHKYDMVEV 652
               +N FSH +K  K G+ G +RIFP+ G++              TPD+V H+Y+MVE+
Sbjct: 601 IVDHVNIFSHLLKGKKTGRGGCVRIFPTAGEIWAVYKNWSLNWDGSTPDEVRHQYEMVEI 660

BLAST of PI0027020 vs. TAIR 10
Match: AT5G35753.1 (Domain of unknown function (DUF3444) )

HSP 1 Score: 184.9 bits (468), Expect = 2.4e-46
Identity = 189/697 (27.12%), Postives = 283/697 (40.60%), Query Frame = 0

Query: 1   MDCNKDDAIKAKQIAEKKFVEMDIATAVRFALRAHSLYPSLDGLPQFIATLNV--YLILG 60
           M+    DA+KAKQ AE++F E D A A  +ALRA SL+P L+GL Q + T ++  +L   
Sbjct: 1   MEAYTQDALKAKQFAERRFAEKDFAGARSYALRAKSLFPDLEGLSQMLTTNSIRRWLYCS 60

Query: 61  VDPLADEETIRKHYRKLALILHPDKNKSIGADGAFKIVSEAWSCLSDKAKRAAFDYKRNI 120
           +    +   + +H+R           + +G            SC                
Sbjct: 61  IPTRTNALELTEHFRSF---------RKLGV-----------SC---------------- 120

Query: 121 RGMPMKSAEIRSSVPIVRNGFHNPFPNNNLNRWQWRSDDEVRSAPASHPVKPTFWTMCNS 180
                                          + Q R D              TFWT+C  
Sbjct: 121 -------------------------------QLQKRLD--------------TFWTVCTY 180

Query: 181 CKVHFEYLRSYLNHNLICPNCRISFLAVENPSPPFNGN----PPSSPWTFNMQQQASSAY 240
           CKV +EYLR Y+N  L C NCR +F+AVE    P + +    PPS   +         A 
Sbjct: 181 CKVQYEYLRKYVNKRLSCKNCRGAFIAVETGPAPVSASLQYAPPSHTTSNGYGGHGYDAV 240

Query: 241 NHFKKSFNAEKTEFSARGGMDSAGYGSMESMHKSFHSSTSCKLRAAESMQASASSAAKAF 300
           +    +      ++ A G      Y + ES   S +  TS     +  M ++++      
Sbjct: 241 SRMPTNSTYFLGQYPAHG----YEYVTNESYDWSSYVGTSPGNLESNRMSSASNGYPYKL 300

Query: 301 S----FFKSSSPKMKVGHKAGISAAMKEEFSPSEDHTPNKGDAGLASTSFNNSACFAHKG 360
           +      K+S P+ K     G S          E+ T +  ++   +        F H G
Sbjct: 301 NNGVVLIKASRPEKKRNVGLGSSGN-----GFVENITKSNPESKATNLDAKMEHDFKHPG 360

Query: 361 DRRKKKMRISGHKMQGSIKNFLRQIEIDNAGIIKESSGLKSIALKEEGQSLKSLDLLIQG 420
                  R S      + K  + + + D    IK+   L+ + L  E  +  + D     
Sbjct: 361 KSYGLMRRWSSTTGLDTRKILIHKAKTD----IKQR--LEIMRLASEAAATATEDATPLD 420

Query: 421 SFLRGKSVGLNGMDSDKDLNTACSKDELQTTYTLPSSENPDTKDSESFSMSVPDPDFHDF 480
                  VG                D++       S  +P  +      ++VPD DFHDF
Sbjct: 421 EVSASSKVG----------------DDVSRLGKNVSFGHPPVRKING-PITVPDSDFHDF 480

Query: 481 DKDR---------------------------AEIISLKPFKMRISWLNSKSNIELAPLNW 540
           DK+R                            E++S++PFK+ I++L+SK++IE   + W
Sbjct: 481 DKNRLEECFEARQIWAIYDEDDGMPRLYCMVREVLSVQPFKIDIAYLSSKTDIEFGTMKW 540

Query: 541 IGCGFPKTSGDFWIGKHEDYGSLNSFSHKVKQIK-GKRGAIRIFPSKGDL---------- 600
           +  GF K+ G F I   +    +N FSH +K  K G+ G +RIFP  GD+          
Sbjct: 541 VQYGFTKSCGHFRIRNTDIVDHVNIFSHLLKGKKTGRGGCVRIFPQTGDIWTVYKNWSPN 583

Query: 601 ----TPDDVIHKYDMVEVLEDYNEDKGVAVVPLVKVVGFKTVFQQHSNPSKIRNIPREEM 646
               TPD+V H+Y MVE+L++Y+E  GV + PLVKV G+KTV+ +       + IPR EM
Sbjct: 601 WNNSTPDEVRHQYKMVEILDEYSEQFGVCIAPLVKVDGYKTVYCRRDKEESKKWIPRREM 583