PI0027005 (gene) Melon (PI 482460) v1

Overview
NamePI0027005
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionBeta-fructofuranosidase, insoluble isoenzyme CWINV1-like
Locationchr01: 28709344 .. 28713839 (-)
RNA-Seq ExpressionPI0027005
SyntenyPI0027005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCCTCTCTCTCTAAAAAAGACCAATCAAAATCAGAATTTTTGTTATAAATAGATAGCTGAAAAACCCAATACTGACTTAACAATTTCCTCTCTAATGGCTTCCATCTCTCTCTGTTTCATCTCTTTTCTCTGTTTCTTGTTTGGCCATGGAGTTTTTCAACTTCAAGCTTCTCACCATGTTTACAAAGCTCAAACTTCTAACTCTCTTCCTTTTCATTATCATCAACAACCTTATAGAACTTCCTACCATTTTCAACCTCCCAAGAATTGGATCAATGGTTTCTTTTCTCTCTCTCTCACTTTCTTGTTCTTGATCATTTCTTTCAATTTTTGTTTTTTCCTTCTGGGTTTTTTCTTTCCACTAACTTTTTTTCTTTTACATGTGATTTGGTAATGAAATTCACCTGAATCATTCTCAGATCCAAATGGTTAGTTCCACCTCCTCCGTTTTCTTTTTCTTTTTCCCTTTTTTTAAAATTTTTTTGCTACATTTGTTCAATTTTTCAACATAAATACTAAGATAAGTACAAATTTGGTCCTTGTTTTTTTTTTTTTTTTTTAAATTTAAATTTCAATACCAAAATGTTGGATATTTCAGGTCCAATGATATACAAAGGAATTTATCATCTGTTTTACCAATACAACCCAAAAGGAGCCGTGTGGGGCAACATTGTGTGGGCTCACTCCACATCAACGGACCTGATCAATTGGGAACCCCATGATCACGCCATCTACCCATCTCAGCCCTCCGATATCAACGGCTGCTGGTCGGGATCCGCCACCATCCTCCCCGGCGAGAAACCGGCGATTTTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCCAAGAACCTCTCTGACCCGTATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTCATGGCCCCCACCCCACAAAACCACATCAATGCCAGCTCCTTCCGAGACCCCACCACCGCCTGGCTCGGCCCCGACGGCGAGTGGCGAGTCATCGTTGGAAACAAAGTTCACACTCGTGGACTCGCCCTCATGTACCAAAGTAAAGATTTCGTTAAGTGGGATCAGGTCGTATTAAACTACCATTAAACTTGAAAATACTCTCTAATCCTACTTTCAAACTTTTCTTGAGTTAAAGTTCAAGTAAAGTGACATTTTCTAATATATAATATTATATGTGGTTGAGATAAATGCAACTAAGAATACACTAAGTATATTGTCTTAAGTTTCTATCGAACTTCTAACACTCTTTTGGTTGATGACATATATAGGTTGACCATCCGCTTCATTATGCGGATGGGTCAGGAATGTGGGAATGTCCAGATTTCTACCCAGTAGCAAAGACCGGTCGAAACGGGGTTGACACAATCGTGAATAGAAGAGACGTGAAACATGTATTGAAAGTGAGCTTGGATGACACAAGGCACGACGTTTATACAATTGGAACATATGACTTGAAGAAAGATATTTATGTACCAAACAAAGGATCAATAGAAGGTTACAATGGATTGAGATATGACTATGGGAAGTATTATGCTTCAAAAACTTTCTATGACGGACAAAAGAAGAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCATCAAGTGTTGAAGATGATATCAAGAAAGGATGGTCTGGCATCCAGGTAATGTTTCAAATGTCAAAGTCAATGTTTCAATCCCATTTTCATTTTTCACCATAAAAAAAGAATATTTCTTTTAATAATATATATATATATATTTATTTATTTATTGAAAAGAAAAGTCTTGAGATCTGCCGACATGAAAAGTGATGGATTTGAATTCTATTTTGTTTTTATTTTACTTCAGTTTCTCAAGGGTTTTATGTTTTAGGATAAATCGAGTAATTAAGTTCAATTTTTTAAAATATATATATTTAGTGTAGAATTAAAATAATAATAATAATAATCTAAAAAGTGTACAATTAGTATTATGGTTTATAGATATATTATAAAATACATTTAGAACAAGTGGGTTGTATTTCGAATTCCCTATCTCCATTGTTGTATTAAAATAGATATTTTTTCATAATCCAAAAAAATCAAAATTAAATAGTTATTTTAAAATGTCAAGATTTAAAGTAAGAATAAAATTATTATTATTATATATTTGTAGGGCCAATCACATATGGATTTGATGAAAACTATTTATTTATTTATTTATTTGCAGGCAATTCCAAGGTCTATTTGGTTGGATGCTTCTGGGAAGCAACTAATTCAATGGCCAATTGAGGAGATACAAAAGCTTAGAAAGAACAAAGTTACATTGACAAACAAAGTATTGAAGAAAGGATCATCGACTGAAGTAAAAGGTGTAACACCATCGCAGGTAAAAATTAAGAGTTCGTTTGGATTATCAAACATTTTAATTTTTACTAATTAAATATGCTCTAAATTTAATTGTCTTTTAATTGTTCCATAAAAACACAACAAAATAGATAATGTCACTCTTTAAGGTTTGTTTGGTTTTGTTTTTTTTTGGTATTATTACAGGCAGATGTTGAGGTTTCATTTAAAGTGAAAAACTTTAAGAAAGCTCAAATATTGAAACCTCAATATTGGAAAAACCCACAATTGCTTTGTAGTCAAAAGGGAACAGAAACTAAAAAAGGTGGAGTTGGTCCATTTGGACTTCTAGTTTTGGCTTCAAATGACTTCAAAGAGTACACTTCTGTCTCCTTTACGATATTCAAAAAACAAGGCAAATACATTGTACTTATGTGTAGCGACCAAAGCAGGTATGAGAGTTCATTTAAGGGATGGAACCAACATTATTGTAACATAGATTATTGTGACAGCTTAATTGAATAATTTGTATTACTTTTTGAACTAATGTTTTTTTCGTGGATAGATCTTCGTTAAACCCGACTAATGACAAGACAACATATGGAGCGTTCTTAGATGTGGACCCCATTAGTGAAGATCTTTCACTAAGAAGTTTGGTGAGATTCTTTTTTTTTCACTATTATTTATTTGGTTATATTAAGGTTGTTTTCTTTTGTTAAGTTTTGATAATATATAATCAAGCTGAATTTATGGAATCAATTTGTTCAAAAAAGTTTAAATAGGGGTTTGGCTTTAGGACAAAGAAGTTGTTTAAAAAGTGAATTTACACATGAATTATTTATAGTACACCATTTGCATGCTAATCAATTTAAAAAGTGCATACTTTTGTGTGTATAACGTGCCACAACTTGATATTCATAATTTTATTTTCACCTCACATGAACTCTATGAAGTTTTAATTCATATATATGCATGTATAATCTATGAATATATTTTTTAAATTTATAGTGAAAATGCTAATAAAAAATAAAAAAGTAATGAAAGAAACCTACAATAAACTAGTAATAGGACTCAATTTAAGATTTATTAAAAGTTCACGGACTAAAATTGAACATTTAGAAATACAGGGACTAAAATTGAACAAAGTTTAGAATATAGAAACTAATGATATTTTAATCAAAAATGAACGTGTAGTAGTTAAACAACGTGAAAAAGAAATTTAGTACGGTTTATTAATGTTATAATTGTCAGCTTTTGTTGTTACCAATATAAAGCACTGTCCTTTTTTAAAAAATTGCTATTTAATCGTAGAAATAAGGGAGGAACAAATGCAACTAAACTCGAATCGTATATCTTTCTATAAAGTATTTGATCGTATTTGATCATATTTGTCAACTAATTGCACATGAAAATTTGTGAAACATTTACAGATTGATCACTCGGTTGTTGAGAGCTTTGGATCTAAGGGGAAAGCTTGCATAACAGCAAGAGTTTATCCAACATTAGCCATTGGAGACAATGTTGGTCTCTATGCTTTCAACAATGGAACAGACAGTGTCACCATTACAAAACTCACTGCTTGGAGCATGAAGAAAGCTCGTATCAATTAAATAGAAGACAGAGTTTGATAAACCATACTCTTTTTATGCATTTGGACAAGCCAATTGGCAATTGAATAAAAGATACCTTCTAACCTTTTCTTTTCTTATATATATAATCTATTGTGTGACTTCTGTTGAAACTGTCTTGGATTTGCCACTAAATTATGGGTAGGTTGTGTAATCCATGTGATAACTCTGCCTTTCCCATGTGCTCAAGTTGTTCTCTTTTGTGTTTTCTTGTGCAGATTAAAAAATAAATCAAAATAGAGTGAAACCCACGTTTTATCTCTCACACAAAAGAAGGAAGAAAAATGCTAATGAAACTGGACATTGTTAATCTGAGGATACAATTATCCTTTTGAATGATTTGGTAGTGATCATGAACATCATCATCCTACAAACAAAGGGTCCGAGTAGATAAACACCTGGAGATATATCTCCTTAACTTTTTTATCCTCTCCTATGGAAGGAGTTGGTACAGTTACAATGTTTCGCCTCACTTTAAATGTGACGTGAATTCATTGTTTGAGAATGTCACTTCACAAAAGAAAGTAGAGATTCGAACCAGA

mRNA sequence

CTCCCTCTCTCTCTAAAAAAGACCAATCAAAATCAGAATTTTTGTTATAAATAGATAGCTGAAAAACCCAATACTGACTTAACAATTTCCTCTCTAATGGCTTCCATCTCTCTCTGTTTCATCTCTTTTCTCTGTTTCTTGTTTGGCCATGGAGTTTTTCAACTTCAAGCTTCTCACCATGTTTACAAAGCTCAAACTTCTAACTCTCTTCCTTTTCATTATCATCAACAACCTTATAGAACTTCCTACCATTTTCAACCTCCCAAGAATTGGATCAATGATCCAAATGGTCCAATGATATACAAAGGAATTTATCATCTGTTTTACCAATACAACCCAAAAGGAGCCGTGTGGGGCAACATTGTGTGGGCTCACTCCACATCAACGGACCTGATCAATTGGGAACCCCATGATCACGCCATCTACCCATCTCAGCCCTCCGATATCAACGGCTGCTGGTCGGGATCCGCCACCATCCTCCCCGGCGAGAAACCGGCGATTTTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCCAAGAACCTCTCTGACCCGTATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTCATGGCCCCCACCCCACAAAACCACATCAATGCCAGCTCCTTCCGAGACCCCACCACCGCCTGGCTCGGCCCCGACGGCGAGTGGCGAGTCATCGTTGGAAACAAAGTTCACACTCGTGGACTCGCCCTCATGTACCAAAGTAAAGATTTCGTTAAGTGGGATCAGGTTGACCATCCGCTTCATTATGCGGATGGGTCAGGAATGTGGGAATGTCCAGATTTCTACCCAGTAGCAAAGACCGGTCGAAACGGGGTTGACACAATCGTGAATAGAAGAGACGTGAAACATGTATTGAAAGTGAGCTTGGATGACACAAGGCACGACGTTTATACAATTGGAACATATGACTTGAAGAAAGATATTTATGTACCAAACAAAGGATCAATAGAAGGTTACAATGGATTGAGATATGACTATGGGAAGTATTATGCTTCAAAAACTTTCTATGACGGACAAAAGAAGAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCATCAAGTGTTGAAGATGATATCAAGAAAGGATGGTCTGGCATCCAGGCAATTCCAAGGTCTATTTGGTTGGATGCTTCTGGGAAGCAACTAATTCAATGGCCAATTGAGGAGATACAAAAGCTTAGAAAGAACAAAGTTACATTGACAAACAAAGTATTGAAGAAAGGATCATCGACTGAAGTAAAAGGTGTAACACCATCGCAGGCAGATGTTGAGGTTTCATTTAAAGTGAAAAACTTTAAGAAAGCTCAAATATTGAAACCTCAATATTGGAAAAACCCACAATTGCTTTGTAGTCAAAAGGGAACAGAAACTAAAAAAGGTGGAGTTGGTCCATTTGGACTTCTAGTTTTGGCTTCAAATGACTTCAAAGAGTACACTTCTGTCTCCTTTACGATATTCAAAAAACAAGGCAAATACATTGTACTTATGTGTAGCGACCAAAGCAGATCTTCGTTAAACCCGACTAATGACAAGACAACATATGGAGCGTTCTTAGATGTGGACCCCATTAGTGAAGATCTTTCACTAAGAAGTTTGATTGATCACTCGGTTGTTGAGAGCTTTGGATCTAAGGGGAAAGCTTGCATAACAGCAAGAGTTTATCCAACATTAGCCATTGGAGACAATGTTGGTCTCTATGCTTTCAACAATGGAACAGACAGTGTCACCATTACAAAACTCACTGCTTGGAGCATGAAGAAAGCTCGTATCAATTAAATAGAAGACAGAGTTTGATAAACCATACTCTTTTTATGCATTTGGACAAGCCAATTGGCAATTGAATAAAAGATACCTTCTAACCTTTTCTTTTCTTATATATATAATCTATTGTGTGACTTCTGTTGAAACTGTCTTGGATTTGCCACTAAATTATGGGTAGGTTGTGTAATCCATGTGATAACTCTGCCTTTCCCATGTGCTCAAGTTGTTCTCTTTTGTGTTTTCTTGTGCAGATTAAAAAATAAATCAAAATAGAGTGAAACCCACGTTTTATCTCTCACACAAAAGAAGGAAGAAAAATGCTAATGAAACTGGACATTGTTAATCTGAGGATACAATTATCCTTTTGAATGATTTGGTAGTGATCATGAACATCATCATCCTACAAACAAAGGGTCCGAGTAGATAAACACCTGGAGATATATCTCCTTAACTTTTTTATCCTCTCCTATGGAAGGAGTTGGTACAGTTACAATGTTTCGCCTCACTTTAAATGTGACGTGAATTCATTGTTTGAGAATGTCACTTCACAAAAGAAAGTAGAGATTCGAACCAGA

Coding sequence (CDS)

ATGGCTTCCATCTCTCTCTGTTTCATCTCTTTTCTCTGTTTCTTGTTTGGCCATGGAGTTTTTCAACTTCAAGCTTCTCACCATGTTTACAAAGCTCAAACTTCTAACTCTCTTCCTTTTCATTATCATCAACAACCTTATAGAACTTCCTACCATTTTCAACCTCCCAAGAATTGGATCAATGATCCAAATGGTCCAATGATATACAAAGGAATTTATCATCTGTTTTACCAATACAACCCAAAAGGAGCCGTGTGGGGCAACATTGTGTGGGCTCACTCCACATCAACGGACCTGATCAATTGGGAACCCCATGATCACGCCATCTACCCATCTCAGCCCTCCGATATCAACGGCTGCTGGTCGGGATCCGCCACCATCCTCCCCGGCGAGAAACCGGCGATTTTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCCAAGAACCTCTCTGACCCGTATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTCATGGCCCCCACCCCACAAAACCACATCAATGCCAGCTCCTTCCGAGACCCCACCACCGCCTGGCTCGGCCCCGACGGCGAGTGGCGAGTCATCGTTGGAAACAAAGTTCACACTCGTGGACTCGCCCTCATGTACCAAAGTAAAGATTTCGTTAAGTGGGATCAGGTTGACCATCCGCTTCATTATGCGGATGGGTCAGGAATGTGGGAATGTCCAGATTTCTACCCAGTAGCAAAGACCGGTCGAAACGGGGTTGACACAATCGTGAATAGAAGAGACGTGAAACATGTATTGAAAGTGAGCTTGGATGACACAAGGCACGACGTTTATACAATTGGAACATATGACTTGAAGAAAGATATTTATGTACCAAACAAAGGATCAATAGAAGGTTACAATGGATTGAGATATGACTATGGGAAGTATTATGCTTCAAAAACTTTCTATGACGGACAAAAGAAGAGGAGAGTTTTGTGGGGTTGGGTGAATGAATCATCAAGTGTTGAAGATGATATCAAGAAAGGATGGTCTGGCATCCAGGCAATTCCAAGGTCTATTTGGTTGGATGCTTCTGGGAAGCAACTAATTCAATGGCCAATTGAGGAGATACAAAAGCTTAGAAAGAACAAAGTTACATTGACAAACAAAGTATTGAAGAAAGGATCATCGACTGAAGTAAAAGGTGTAACACCATCGCAGGCAGATGTTGAGGTTTCATTTAAAGTGAAAAACTTTAAGAAAGCTCAAATATTGAAACCTCAATATTGGAAAAACCCACAATTGCTTTGTAGTCAAAAGGGAACAGAAACTAAAAAAGGTGGAGTTGGTCCATTTGGACTTCTAGTTTTGGCTTCAAATGACTTCAAAGAGTACACTTCTGTCTCCTTTACGATATTCAAAAAACAAGGCAAATACATTGTACTTATGTGTAGCGACCAAAGCAGATCTTCGTTAAACCCGACTAATGACAAGACAACATATGGAGCGTTCTTAGATGTGGACCCCATTAGTGAAGATCTTTCACTAAGAAGTTTGATTGATCACTCGGTTGTTGAGAGCTTTGGATCTAAGGGGAAAGCTTGCATAACAGCAAGAGTTTATCCAACATTAGCCATTGGAGACAATGTTGGTCTCTATGCTTTCAACAATGGAACAGACAGTGTCACCATTACAAAACTCACTGCTTGGAGCATGAAGAAAGCTCGTATCAATTAA

Protein sequence

MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHYHQQPYRTSYHFQPPKNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGMWECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKAQILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN
Homology
BLAST of PI0027005 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 2.3e-227
Identity = 384/573 (67.02%), Postives = 446/573 (77.84%), Query Frame = 0

Query: 12  LCFLFGHGVFQLQASHHVYKAQT-SNSLPFHYHQQPYRTSYHFQPPKNWINDPNGPMIYK 71
           L  L G+ V  L+ASHHVYK  T S +       QPYRT +HFQPPKNW+NDPNGPMIYK
Sbjct: 15  LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYK 74

Query: 72  GIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPG 131
           GIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AI+PS P DINGCWSGSATILP 
Sbjct: 75  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 134

Query: 132 EKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINASSFRDPT 191
            KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP   N INASSFRDPT
Sbjct: 135 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 194

Query: 192 TAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGMWECPDFYPVA 251
           TAWLG D +WRVI+G+K+H RGLA+ Y SKDF+KW++   PLHY DGSGMWECPDF+PV 
Sbjct: 195 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 254

Query: 252 KTGRNGVDTI---VNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGSIEGYNGL 311
           + G NGV+T         +KHVLK+SLDDT+HD YTIGTYD  KD +VP+ G        
Sbjct: 255 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 314

Query: 312 RYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWLDASGKQL 371
           RYDYGKYYASKTF+D  K RR+LWGW NESSSVEDD++KGWSGIQ IPR IWLD SGKQL
Sbjct: 315 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 374

Query: 372 IQWPIEEIQKLRKNKV-TLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKAQILKPQ 431
           IQWP+ E+++LR  +V  L NKVLK GS  EV GVT +QADVEV FKV++ +KA +++P 
Sbjct: 375 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 434

Query: 432 YWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFK---KQGKYIVLMCS 491
            W +PQL+CS+      K G+GPFGL+VLAS + +EYTSV F IFK      KY+VLMCS
Sbjct: 435 -WTDPQLICSKMNVSV-KSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCS 494

Query: 492 DQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYPTLA 551
           DQSRSSL   NDKTTYGAF+D++P  + LSLR+LIDHSVVESFG KG+ACIT+RVYP LA
Sbjct: 495 DQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLA 554

Query: 552 IGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           IG +  L+AFN G  SV +  L AWSM  A+I+
Sbjct: 555 IGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of PI0027005 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 2.1e-212
Identity = 361/557 (64.81%), Postives = 425/557 (76.30%), Query Frame = 0

Query: 1   MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHY----HQQPYRTSYHFQPP 60
           MA  S+  +S    ++   V  ++A+HHVY  QT  +L  H+    + QPYRT+YHFQP 
Sbjct: 1   MAISSIFLLSLFSLIY---VIPIEATHHVY--QTLETLSSHHSSKSNHQPYRTAYHFQPL 60

Query: 61  KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSD 120
           KNWINDPNGPM Y G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P DHAI+PSQPSD
Sbjct: 61  KNWINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSD 120

Query: 121 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 180
           I GCWSGSATILPG KPAILYTGI+P N QVQN+A+PKN+SDP LREW KSPKNPLM PT
Sbjct: 121 IKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPT 180

Query: 181 PQNHINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYAD 240
             N IN+SSFRDPTT+WLG DG WRV++G+K+ T+G+A++Y+SK+FV W +  HPLH A+
Sbjct: 181 IANKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAE 240

Query: 241 GSGMWECPDFYPVAKTG--RNGVDTIVN-RRDVKHVLKVSLDDTRHDVYTIGTYDLKKDI 300
           G+GMWECPDFYPV      R GVDT  N   DV+HVLKVSLDDT+HD Y IG+YD+ KD+
Sbjct: 241 GTGMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDV 300

Query: 301 YVPNKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQA 360
           +VP  G  +    LRYDYGKYYASKTF+D  K RR+L GWVNESSSV DD+KKGWSGI  
Sbjct: 301 FVPENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHT 360

Query: 361 IPRSIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFK 420
           IPR IWL  SGKQL+QWP++EI+ LR N V    KV+K G    + GV   QADVE+SF+
Sbjct: 361 IPREIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFE 420

Query: 421 VKNFKKAQILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKK 480
           + +  K + L+   W +PQLLCSQKG    KGGVGPFGLLV AS   KEYT+V F IFK 
Sbjct: 421 ISDLGKVESLRK--WIDPQLLCSQKGAGV-KGGVGPFGLLVFASQGLKEYTAVFFRIFKY 480

Query: 481 QGKYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKAC 540
           Q K +VLMCSDQSRSSLN  ND T+YG F+DVDP+ E LSLR+LIDHSVVESFG +G+AC
Sbjct: 481 QDKNLVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRAC 540

Query: 541 ITARVYPTLAIGDNVGL 551
           +TARVYPTLAI D   L
Sbjct: 541 VTARVYPTLAIHDKAML 549

BLAST of PI0027005 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 6.3e-209
Identity = 360/579 (62.18%), Postives = 432/579 (74.61%), Query Frame = 0

Query: 9   ISFLCFLFGHGVFQL---QASHHVYKAQTSNSLPFHYHQ-----QPYRTSYHFQPPKNWI 68
           +S    L  HG+  +   QA HH       + +  + HQ      PYRT+YHFQ PKNW+
Sbjct: 11  LSISMMLLSHGMIIIAKDQAIHH--HDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWM 70

Query: 69  NDPNGPMIYKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWEPHDHAIYPSQPSDING 128
           NDPNGPMIYKGIYHLFYQY P   VW   IVW HSTSTDLINW     A+ PS+P DING
Sbjct: 71  NDPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDING 130

Query: 129 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMA--PTP 188
           CWSGS TILP  KP ILYTGIN KN QVQNLA+PKNLSDPYL+EW+K P+NPLMA  PT 
Sbjct: 131 CWSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTN 190

Query: 189 QNHINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADG 248
            N+INASSFRDP+TAW   DG+WRVIVG +   RGLA+++ S DFVKW+   +PLH  +G
Sbjct: 191 NNNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEG 250

Query: 249 SGMWECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPN 308
           +G+WECPDF+PV      G DT +   DVKHVLK+SL DT+++ YTIG YD++KDIYVP+
Sbjct: 251 NGIWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVPD 310

Query: 309 KGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRS 368
           +GSIE   GLRYDYGK+YASK+F+D +  RR+LWGWVNESS   DDIKKGWSG+QAIPR+
Sbjct: 311 EGSIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPRT 370

Query: 369 IWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNF 428
           + LD SGKQL+QWP+ E+  LR+N V L ++V+K GS  E+  +T SQADVE+SFK+   
Sbjct: 371 VVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPES 430

Query: 429 KKAQILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKY 488
              + L      NPQ+LCSQKG    KG  GPFGLL LAS   +EYT+V F IFK   KY
Sbjct: 431 NYVEEL-DSTCTNPQILCSQKGASI-KGRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKY 490

Query: 489 IVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITAR 548
           +VLMCSDQ+RSSLNPT DK ++G F+DVDPI+EDLSLR LIDHS+VESF +KGK+CITAR
Sbjct: 491 VVLMCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITAR 550

Query: 549 VYPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           VYPT+AI D   LY FNNGT+ V ITKL+AWSMKKA+IN
Sbjct: 551 VYPTMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQIN 585

BLAST of PI0027005 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 724.9 bits (1870), Expect = 7.0e-208
Identity = 348/541 (64.33%), Postives = 413/541 (76.34%), Query Frame = 0

Query: 45  QPYRTSYHFQPPKNWINDPNGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWE 104
           QPYRT YHFQP KNW+NDPNGPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW 
Sbjct: 36  QPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWI 95

Query: 105 PHDHAIYPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLRE 164
               A  PSQPSDINGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLRE
Sbjct: 96  SQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLRE 155

Query: 165 WVKSPKNPLMAPTPQNHINASSFRDPTTAWLGPDGEWRVIVGNKV-HTRGLALMYQSKDF 224
           W K P+NPLM     N IN   FRDPTTAWLG DGEWRVIVG+     RGLA++Y+S+DF
Sbjct: 156 WSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDF 215

Query: 225 VKWDQVDHPLHYADGSGMWECPDFYPVAKTGRNGVDT-IVNRRDVKHVLKVSLDDTRHDV 284
             W Q   PLHY D +GMWECPDF+PV+ TG +GV+T  V    +KHVLKVSL +T HD 
Sbjct: 216 FNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDY 275

Query: 285 YTIGTYDLKKDIYVPNKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVE 344
           YTIG+YD +KD+YVP+ G ++  +  R DYGKYYASKTFYD  KKRR+LWGWVNESS  +
Sbjct: 276 YTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAK 335

Query: 345 DDIKKGWSGIQAIPRSIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGV 404
           DDI+KGWSG+Q+ PR IWLD SGK+L+QWPIEEI+ LR  +V    KVLK GS+ +V GV
Sbjct: 336 DDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGV 395

Query: 405 TPSQADVEVSFKVKNFKKAQILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFK 464
           T +QADVEVSFKVK  +KA +++P  W +PQ +CSQ G  +   G+GPFGL+VLASND +
Sbjct: 396 TAAQADVEVSFKVKELEKADVIEPS-WTDPQKICSQ-GDLSVMSGLGPFGLMVLASNDME 455

Query: 465 EYTSVSFTIFK------KQGKYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLR 524
           EYTSV F IFK      K+ KY+VLMCSDQSRSSLN  NDK+T+GAF+ +DP  + +SLR
Sbjct: 456 EYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLR 515

Query: 525 SLIDHSVVESFGSKGKACITARVYPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARI 577
           +LIDHS+VES+G  G+ CIT+RVYP LAIG+N  L+ FN GT SV I  L+AWS+K A+I
Sbjct: 516 TLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 574

BLAST of PI0027005 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 698.7 bits (1802), Expect = 5.4e-200
Identity = 334/546 (61.17%), Postives = 414/546 (75.82%), Query Frame = 0

Query: 36  NSLPFHYHQQPYRTSYHFQPPKNWINDPNGPMIYKGIYHLFYQYNPKGAVW--GNIVWAH 95
           +++P +   QPYRT YHFQPPKNW+NDPNGPMIYKGIYHLFYQ+N  GAV      VW H
Sbjct: 31  DNVPNNIKNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGH 90

Query: 96  STSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVP 155
           +TSTDLINW     AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  P
Sbjct: 91  ATSTDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKP 150

Query: 156 KNLSDPYLREWVKSPKNPLMAPTPQNHINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGL 215
           KNL+DPYLR W KSP+NPL+ P+P NHIN+S+FRDPTTAW G DG WR+  G++   RGL
Sbjct: 151 KNLTDPYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGL 210

Query: 216 ALMYQSKDFVKWDQVDHPLHYADGSGMWECPDFYPVAKTGRNGVDTIVNRRD-VKHVLKV 275
           A+++ SKDFV W Q   PLHY DG+GMWECPDF+PVA+T   G+DT  +    VKHVLKV
Sbjct: 211 AILHTSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKV 270

Query: 276 SLDDTRHDVYTIGTYDLKKDIYVPNKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWG 335
           SL DT +D YTIGTYD  +D+YVP+KG ++     RYDYGK+YASKTFYD   +RR+LWG
Sbjct: 271 SLTDTFNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWG 330

Query: 336 WVNESSSVEDDIKKGWSGIQAIPRSIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKK 395
           WVNESS  +D+IKKGW+G+QAIPR +WLD SGK+L+QWP++EI++LR  +V   NK+LK 
Sbjct: 331 WVNESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKG 390

Query: 396 GSSTEVKGVTPSQADVEVSFKVKNF--KKAQILKPQYWKNPQLLCSQKGTETKKGGVGPF 455
           GS  EV GVT  QADVEV FKV  F  +KA +++P  W +PQL+CSQ+   +   G+GPF
Sbjct: 391 GSVMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPG-WTDPQLICSQRNASS---GLGPF 450

Query: 456 GLLVLASNDFKEYTSVSFTIFK---KQGKYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDP 515
           GL+VLAS + +EYTSV+  IF+      +++V+MCSDQS SSL   NDKTTYGAFLD+ P
Sbjct: 451 GLMVLASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP 510

Query: 516 ISEDLSLRSLIDHSVVESFGSKGKACITARVYPTLAIGDNVGLYAFNNGTDSVTITKLTA 574
             + +SLR+LID S+VESFG KGK CIT+RVYP LAIG+   L+AFN G+ +V +  L+A
Sbjct: 511 Y-QPISLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSA 570

BLAST of PI0027005 vs. ExPASy TrEMBL
Match: A0A0A0K761 (Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 540/578 (93.43%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHY-HQQPYRTSYHFQPPKNW 60
           MASISLCFISFLCFLFGHGVFQLQASHHV+K QTS SLP+HY H+QPYRTSYHFQPPKNW
Sbjct: 1   MASISLCFISFLCFLFGHGVFQLQASHHVHKPQTSTSLPYHYHHRQPYRTSYHFQPPKNW 60

Query: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDING 120
           INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSDING
Sbjct: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDING 120

Query: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180
           CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN
Sbjct: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180

Query: 181 HINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSG 240
            INASSFRDPTTAWLGP+GEWRVIVGNKVHTRGLALM+QSKDF+KWDQVDHPLHYADGSG
Sbjct: 181 QINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYADGSG 240

Query: 241 MWECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKG 300
           MWECPDFYPVAKTGR GVDT VN ++VKHVLKVSLDDTRHDVYTIGTYD++KDIYVPNKG
Sbjct: 241 MWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVPNKG 300

Query: 301 SIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIW 360
           SIEGY+GLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQ IPR+IW
Sbjct: 301 SIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPRTIW 360

Query: 361 LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKK 420
           LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGS+ EVKGVTPSQADVEVSFKVKN KK
Sbjct: 361 LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKNLKK 420

Query: 421 AQILKPQYWKNPQLLCSQKG-TETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYI 480
           AQILKPQYW+NPQ+LCSQKG   T KGGVGPFGLLVLASNDFKEYTSVSFTIFKKQ KY+
Sbjct: 421 AQILKPQYWENPQVLCSQKGRAATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQSKYV 480

Query: 481 VLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARV 540
            LMCSDQSRSSLNPTNDKTTYGAFLDVD  SEDLSLRSLIDHSVVESFG KGK CITARV
Sbjct: 481 ALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCITARV 540

Query: 541 YPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           YPTLAIGDNVGLYAFNNGT+SVTITKLTAWSMKKARIN
Sbjct: 541 YPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 578

BLAST of PI0027005 vs. ExPASy TrEMBL
Match: A0A5A7UWG3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00970 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 5.1e-294
Identity = 481/515 (93.40%), Postives = 502/515 (97.48%), Query Frame = 0

Query: 62  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 121
           D +GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW
Sbjct: 3   DISGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 62

Query: 122 SGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 181
           SGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI
Sbjct: 63  SGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 122

Query: 182 NASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGMW 241
           NASSFRDPTTAWLG DGEWRVI+G+KVHTRGLALMYQSKDFVKW QVDHPLHYA+G+GMW
Sbjct: 123 NASSFRDPTTAWLGRDGEWRVIIGSKVHTRGLALMYQSKDFVKWKQVDHPLHYAEGTGMW 182

Query: 242 ECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGSI 301
           ECPDF+PVAKTGR+GVDT V+ ++ KHVLK+SLDDT+HDVYTIGTYDL+KD+YVPNKGSI
Sbjct: 183 ECPDFFPVAKTGRSGVDTTVSGKNTKHVLKISLDDTKHDVYTIGTYDLEKDVYVPNKGSI 242

Query: 302 EGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWLD 361
           EGYN LRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPR++WLD
Sbjct: 243 EGYNALRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTVWLD 302

Query: 362 ASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKAQ 421
           ASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGS+ EVKGVTPSQADVEVSFKVKNFKKAQ
Sbjct: 303 ASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKNFKKAQ 362

Query: 422 ILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYIVLM 481
           ILKPQYW+NPQLLCSQKGT T KGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKY+VLM
Sbjct: 363 ILKPQYWENPQLLCSQKGTATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYVVLM 422

Query: 482 CSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYPT 541
           CSDQSRSSLNPTNDKTTYGAFLDVD +SEDLSLRSLIDHSVVESFGSKGKACITARVYPT
Sbjct: 423 CSDQSRSSLNPTNDKTTYGAFLDVDLVSEDLSLRSLIDHSVVESFGSKGKACITARVYPT 482

Query: 542 LAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           LAIGDNVGLYAFNNGTDSV ITKLTAWSMKKA I+
Sbjct: 483 LAIGDNVGLYAFNNGTDSVNITKLTAWSMKKAVIS 517

BLAST of PI0027005 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.6e-294
Identity = 484/576 (84.03%), Postives = 527/576 (91.49%), Query Frame = 0

Query: 1   MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHYHQQPYRTSYHFQPPKNWI 60
           MAS SLCFISFLCFL  HG  +LQASHHVY  Q S  L  H ++QPYRTSYHFQPPKNWI
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLL-LHRYEQPYRTSYHFQPPKNWI 60

Query: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC 120
           NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGC
Sbjct: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGC 120

Query: 121 WSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180
           WSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH
Sbjct: 121 WSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180

Query: 181 INASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGM 240
           IN+SSFRDPTTAWLG DGEWRVI+G+K+  RGLA+M+QSKDFVKWDQVDHPLHYADG+GM
Sbjct: 181 INSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGM 240

Query: 241 WECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGS 300
           WECPDF+PVAK  R GVDT V+ + VKHVLKVSLDDT+HD YT+GTYDLKKDIYVP+KGS
Sbjct: 241 WECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGS 300

Query: 301 IEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWL 360
           IEGY GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSV+DDIKKGWSG+QAIPR++WL
Sbjct: 301 IEGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWL 360

Query: 361 DASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKA 420
           DASGKQLIQWPI EIQKLRK+KV LTNKVLKKGS   VKGVT +QADV+VSFKVK+F KA
Sbjct: 361 DASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKA 420

Query: 421 QILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYIVL 480
           Q LK + WK+PQL+CSQ G    KGGVGPFGLLVLASN+ +EYTSV F IFK+Q KY+VL
Sbjct: 421 QPLKAE-WKDPQLVCSQMGA-AAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVL 480

Query: 481 MCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYP 540
           MCSDQSRSSLN  NDKTTYGAFLDVDP+ E++SLRSLIDHS+VESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
            LAIGD  GLYAFNNGT+SVTITKL+AWSMKKA+I+
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of PI0027005 vs. ExPASy TrEMBL
Match: A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 1.8e-291
Identity = 478/575 (83.13%), Postives = 525/575 (91.30%), Query Frame = 0

Query: 1   MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHYHQQPYRTSYHFQPPKNWI 60
           MAS SLCFISFLCFL  HG  +LQASHHVY  Q S  L  H+H+QPYRTS HFQPPKNWI
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLL-LHHHEQPYRTSCHFQPPKNWI 60

Query: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC 120
           NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGC
Sbjct: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGC 120

Query: 121 WSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180
           WSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH
Sbjct: 121 WSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180

Query: 181 INASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGM 240
           IN+SSFRDPTTAWLGPDGEWRVI+G+KV  RGLA+M++SKDFVKWDQVDHPLHYADG GM
Sbjct: 181 INSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGM 240

Query: 241 WECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGS 300
           WECPDF+PVAK GR GVDT V+ + VKHVLKVSLDDT+HD YT+GTYDLKKDIYVP+KGS
Sbjct: 241 WECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGS 300

Query: 301 IEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWL 360
           I+ Y+GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSV+DDIKKGWSG+QAIPR++WL
Sbjct: 301 IQDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWL 360

Query: 361 DASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKA 420
           DASGKQLIQWPI EIQKLRK+K+ LTNKVL+KGS   VKGVT +QADV+VSFKVK+F KA
Sbjct: 361 DASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKA 420

Query: 421 QILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYIVL 480
            +LK + WK+PQL+CSQ G    KGGVGPFGLLVLASN+ +EYTSV F IFK+Q KY+VL
Sbjct: 421 HLLKAE-WKDPQLVCSQMGA-AAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVL 480

Query: 481 MCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYP 540
           MCSDQSRSSLN  NDKT YGAFLDVDP+ +++SLRSLIDHS+VESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARI 576
            LAIGD  GLYAFNNGT+SVTITKL AWS+KKA+I
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 572

BLAST of PI0027005 vs. ExPASy TrEMBL
Match: A0A2I4JRK2 (Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.7e-289
Identity = 479/582 (82.30%), Postives = 522/582 (89.69%), Query Frame = 0

Query: 3   SISLCFIS----FLCFLFGHGVFQLQASHHVYKA--QTSNSLPFHYHQQPYRTSYHFQPP 62
           S SLCF++     +  LFGH   QLQA HHVY+A  +TS+ +   + QQPYRTSYHFQPP
Sbjct: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64

Query: 63  KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSD 122
           KNWINDPNGPMIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSD
Sbjct: 65  KNWINDPNGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124

Query: 123 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182
           INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184

Query: 183 PQNHINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYAD 242
           PQNHIN SSFRDPTTAWLG DGEWRVI+G+K+  RGLA+MY+SKDFV+W+QVDHPL Y D
Sbjct: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244

Query: 243 GSGMWECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVP 302
             GMWECPDF+PVAKTGR GV+  V+   VKH+LKVSLDDT+HDVYT+GTY+ KKDIY+P
Sbjct: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304

Query: 303 NKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPR 362
           NKGSIEGYNGLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+QAIPR
Sbjct: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364

Query: 363 SIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKN 422
           +IWLDASGKQLIQWPI EIQKLR NKV LTN+VLKKGS+ EVKGVT +QADVE+SFKV+N
Sbjct: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVEN 424

Query: 423 FKKAQILKPQYWKNPQLLCSQKGTE--TKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQ 482
            KKA+ILKP+ WK+PQLLCSQKGT   T+ G VGPFGLLVLASND KEYTSV FT+FKK 
Sbjct: 425 LKKAEILKPE-WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKP 484

Query: 483 GKYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACI 542
            KY+VLMCSDQSRSSL+  NDKTTYGAFLDVDP  E++SLRSLIDHS+VESFG KGK CI
Sbjct: 485 HKYVVLMCSDQSRSSLHSNNDKTTYGAFLDVDPTRENISLRSLIDHSIVESFGIKGKGCI 544

Query: 543 TARVYPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           TARVYPTLAIGDNV LYAFNNGTDSVTITKLTAWSMKKA IN
Sbjct: 545 TARVYPTLAIGDNVSLYAFNNGTDSVTITKLTAWSMKKAHIN 585

BLAST of PI0027005 vs. NCBI nr
Match: XP_004141685.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV1 [Cucumis sativus] >ALS30216.1 cell wall invertase 5 [Cucumis sativus])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 540/578 (93.43%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHY-HQQPYRTSYHFQPPKNW 60
           MASISLCFISFLCFLFGHGVFQLQASHHV+K QTS SLP+HY H+QPYRTSYHFQPPKNW
Sbjct: 1   MASISLCFISFLCFLFGHGVFQLQASHHVHKPQTSTSLPYHYHHRQPYRTSYHFQPPKNW 60

Query: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDING 120
           INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSDING
Sbjct: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDING 120

Query: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180
           CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN
Sbjct: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180

Query: 181 HINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSG 240
            INASSFRDPTTAWLGP+GEWRVIVGNKVHTRGLALM+QSKDF+KWDQVDHPLHYADGSG
Sbjct: 181 QINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYADGSG 240

Query: 241 MWECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKG 300
           MWECPDFYPVAKTGR GVDT VN ++VKHVLKVSLDDTRHDVYTIGTYD++KDIYVPNKG
Sbjct: 241 MWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVPNKG 300

Query: 301 SIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIW 360
           SIEGY+GLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQ IPR+IW
Sbjct: 301 SIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPRTIW 360

Query: 361 LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKK 420
           LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGS+ EVKGVTPSQADVEVSFKVKN KK
Sbjct: 361 LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKNLKK 420

Query: 421 AQILKPQYWKNPQLLCSQKG-TETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYI 480
           AQILKPQYW+NPQ+LCSQKG   T KGGVGPFGLLVLASNDFKEYTSVSFTIFKKQ KY+
Sbjct: 421 AQILKPQYWENPQVLCSQKGRAATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQSKYV 480

Query: 481 VLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARV 540
            LMCSDQSRSSLNPTNDKTTYGAFLDVD  SEDLSLRSLIDHSVVESFG KGK CITARV
Sbjct: 481 ALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCITARV 540

Query: 541 YPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           YPTLAIGDNVGLYAFNNGT+SVTITKLTAWSMKKARIN
Sbjct: 541 YPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 578

BLAST of PI0027005 vs. NCBI nr
Match: XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 522/576 (90.62%), Postives = 548/576 (95.14%), Query Frame = 0

Query: 1   MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHYHQQPYRTSYHFQPPKNWI 60
           MAS SLCFISFLCFLFGHGV QLQASHHVY+AQ SNSLP HY QQPYRTSYHFQPPKNWI
Sbjct: 1   MASFSLCFISFLCFLFGHGVLQLQASHHVYRAQLSNSLPHHY-QQPYRTSYHFQPPKNWI 60

Query: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC 120
           NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC
Sbjct: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC 120

Query: 121 WSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180
           WSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH
Sbjct: 121 WSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180

Query: 181 INASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGM 240
           IN+SSFRDPTTAWLGPDGEWRVI+G+K++ RGLA+MYQSKDFVKWDQVDHPLHYADG+GM
Sbjct: 181 INSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPLHYADGTGM 240

Query: 241 WECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGS 300
           WECPDF+PVAKTGR GVDT V+   VKHVLKVSLDDT+HD YT+G YDL+KDIYVPNKGS
Sbjct: 241 WECPDFFPVAKTGRKGVDTSVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKDIYVPNKGS 300

Query: 301 IEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWL 360
           IEGY GLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPR+IWL
Sbjct: 301 IEGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTIWL 360

Query: 361 DASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKA 420
           DASGKQLIQWPIEEIQKLRK+KV LTNKVLKKGS  EVKGVT +QADVEVSFKVKNFKKA
Sbjct: 361 DASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSFKVKNFKKA 420

Query: 421 QILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYIVL 480
           QILKP+ WK+PQLLCSQKG  T KGGVG FGLLVLAS D KEYTS+SFTIFK+QGKYIVL
Sbjct: 421 QILKPE-WKDPQLLCSQKGA-TTKGGVGSFGLLVLASKDLKEYTSISFTIFKRQGKYIVL 480

Query: 481 MCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYP 540
           MCSDQSRSSLNP NDKTTYGAFL+VDP+SEDLSLRSLIDHSVVESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLIDHSVVESFGSKGKACITARVYP 540

Query: 541 TLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           TLAIGDNVGLY FNNGT+SVTITKL AWS+KKA+IN
Sbjct: 541 TLAIGDNVGLYVFNNGTESVTITKLGAWSLKKAQIN 573

BLAST of PI0027005 vs. NCBI nr
Match: KAE8646511.1 (hypothetical protein Csa_015867 [Cucumis sativus])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 522/581 (89.85%), Postives = 545/581 (93.80%), Query Frame = 0

Query: 3   SISLCFIS----FLCFLFGHGVFQLQASHHVYKA--QTSNSLPFHYHQQPYRTSYHFQPP 62
           S SLCF++     +  LFGH   QLQA HHVY+A  +TS+ +   + QQPYRTSYHFQPP
Sbjct: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64

Query: 63  KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSD 122
           KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSD
Sbjct: 65  KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124

Query: 123 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182
           INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184

Query: 183 PQNHINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYAD 242
           PQN INASSFRDPTTAWLGP+GEWRVIVGNKVHTRGLALM+QSKDF+KWDQVDHPLHYAD
Sbjct: 185 PQNQINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYAD 244

Query: 243 GSGMWECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVP 302
           GSGMWECPDFYPVAKTGR GVDT VN ++VKHVLKVSLDDTRHDVYTIGTYD++KDIYVP
Sbjct: 245 GSGMWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVP 304

Query: 303 NKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPR 362
           NKGSIEGY+GLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQ IPR
Sbjct: 305 NKGSIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPR 364

Query: 363 SIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKN 422
           +IWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGS+ EVKGVTPSQADVEVSFKVKN
Sbjct: 365 TIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKN 424

Query: 423 FKKAQILKPQYWKNPQLLCSQKG-TETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQG 482
            KKAQILKPQYW+NPQ+LCSQKG   T KGGVGPFGLLVLASNDFKEYTSVSFTIFKKQ 
Sbjct: 425 LKKAQILKPQYWENPQVLCSQKGRAATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQS 484

Query: 483 KYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACIT 542
           KY+ LMCSDQSRSSLNPTNDKTTYGAFLDVD  SEDLSLRSLIDHSVVESFG KGK CIT
Sbjct: 485 KYVALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCIT 544

Query: 543 ARVYPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           ARVYPTLAIGDNVGLYAFNNGT+SVTITKLTAWSMKKARIN
Sbjct: 545 ARVYPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 585

BLAST of PI0027005 vs. NCBI nr
Match: KAA0059424.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucumis melo var. makuwa] >TYK03901.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1019.6 bits (2635), Expect = 1.0e-293
Identity = 481/515 (93.40%), Postives = 502/515 (97.48%), Query Frame = 0

Query: 62  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 121
           D +GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW
Sbjct: 3   DISGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 62

Query: 122 SGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 181
           SGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI
Sbjct: 63  SGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 122

Query: 182 NASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGMW 241
           NASSFRDPTTAWLG DGEWRVI+G+KVHTRGLALMYQSKDFVKW QVDHPLHYA+G+GMW
Sbjct: 123 NASSFRDPTTAWLGRDGEWRVIIGSKVHTRGLALMYQSKDFVKWKQVDHPLHYAEGTGMW 182

Query: 242 ECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGSI 301
           ECPDF+PVAKTGR+GVDT V+ ++ KHVLK+SLDDT+HDVYTIGTYDL+KD+YVPNKGSI
Sbjct: 183 ECPDFFPVAKTGRSGVDTTVSGKNTKHVLKISLDDTKHDVYTIGTYDLEKDVYVPNKGSI 242

Query: 302 EGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWLD 361
           EGYN LRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPR++WLD
Sbjct: 243 EGYNALRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTVWLD 302

Query: 362 ASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKAQ 421
           ASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGS+ EVKGVTPSQADVEVSFKVKNFKKAQ
Sbjct: 303 ASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKNFKKAQ 362

Query: 422 ILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYIVLM 481
           ILKPQYW+NPQLLCSQKGT T KGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKY+VLM
Sbjct: 363 ILKPQYWENPQLLCSQKGTATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYVVLM 422

Query: 482 CSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYPT 541
           CSDQSRSSLNPTNDKTTYGAFLDVD +SEDLSLRSLIDHSVVESFGSKGKACITARVYPT
Sbjct: 423 CSDQSRSSLNPTNDKTTYGAFLDVDLVSEDLSLRSLIDHSVVESFGSKGKACITARVYPT 482

Query: 542 LAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           LAIGDNVGLYAFNNGTDSV ITKLTAWSMKKA I+
Sbjct: 483 LAIGDNVGLYAFNNGTDSVNITKLTAWSMKKAVIS 517

BLAST of PI0027005 vs. NCBI nr
Match: XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.4e-293
Identity = 484/576 (84.03%), Postives = 527/576 (91.49%), Query Frame = 0

Query: 1   MASISLCFISFLCFLFGHGVFQLQASHHVYKAQTSNSLPFHYHQQPYRTSYHFQPPKNWI 60
           MAS SLCFISFLCFL  HG  +LQASHHVY  Q S  L  H ++QPYRTSYHFQPPKNWI
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLL-LHRYEQPYRTSYHFQPPKNWI 60

Query: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC 120
           NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGC
Sbjct: 61  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGC 120

Query: 121 WSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180
           WSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH
Sbjct: 121 WSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNH 180

Query: 181 INASSFRDPTTAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGM 240
           IN+SSFRDPTTAWLG DGEWRVI+G+K+  RGLA+M+QSKDFVKWDQVDHPLHYADG+GM
Sbjct: 181 INSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGM 240

Query: 241 WECPDFYPVAKTGRNGVDTIVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGS 300
           WECPDF+PVAK  R GVDT V+ + VKHVLKVSLDDT+HD YT+GTYDLKKDIYVP+KGS
Sbjct: 241 WECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGS 300

Query: 301 IEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWL 360
           IEGY GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSV+DDIKKGWSG+QAIPR++WL
Sbjct: 301 IEGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWL 360

Query: 361 DASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKA 420
           DASGKQLIQWPI EIQKLRK+KV LTNKVLKKGS   VKGVT +QADV+VSFKVK+F KA
Sbjct: 361 DASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKA 420

Query: 421 QILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQGKYIVL 480
           Q LK + WK+PQL+CSQ G    KGGVGPFGLLVLASN+ +EYTSV F IFK+Q KY+VL
Sbjct: 421 QPLKAE-WKDPQLVCSQMGA-AAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVL 480

Query: 481 MCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYP 540
           MCSDQSRSSLN  NDKTTYGAFLDVDP+ E++SLRSLIDHS+VESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
            LAIGD  GLYAFNNGT+SVTITKL+AWSMKKA+I+
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of PI0027005 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 789.6 bits (2038), Expect = 1.6e-228
Identity = 384/573 (67.02%), Postives = 446/573 (77.84%), Query Frame = 0

Query: 12  LCFLFGHGVFQLQASHHVYKAQT-SNSLPFHYHQQPYRTSYHFQPPKNWINDPNGPMIYK 71
           L  L G+ V  L+ASHHVYK  T S +       QPYRT +HFQPPKNW+NDPNGPMIYK
Sbjct: 15  LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYK 74

Query: 72  GIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPG 131
           GIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AI+PS P DINGCWSGSATILP 
Sbjct: 75  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 134

Query: 132 EKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINASSFRDPT 191
            KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP   N INASSFRDPT
Sbjct: 135 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 194

Query: 192 TAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGMWECPDFYPVA 251
           TAWLG D +WRVI+G+K+H RGLA+ Y SKDF+KW++   PLHY DGSGMWECPDF+PV 
Sbjct: 195 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 254

Query: 252 KTGRNGVDTI---VNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGSIEGYNGL 311
           + G NGV+T         +KHVLK+SLDDT+HD YTIGTYD  KD +VP+ G        
Sbjct: 255 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 314

Query: 312 RYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWLDASGKQL 371
           RYDYGKYYASKTF+D  K RR+LWGW NESSSVEDD++KGWSGIQ IPR IWLD SGKQL
Sbjct: 315 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 374

Query: 372 IQWPIEEIQKLRKNKV-TLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKAQILKPQ 431
           IQWP+ E+++LR  +V  L NKVLK GS  EV GVT +QADVEV FKV++ +KA +++P 
Sbjct: 375 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 434

Query: 432 YWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFK---KQGKYIVLMCS 491
            W +PQL+CS+      K G+GPFGL+VLAS + +EYTSV F IFK      KY+VLMCS
Sbjct: 435 -WTDPQLICSKMNVSV-KSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCS 494

Query: 492 DQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYPTLA 551
           DQSRSSL   NDKTTYGAF+D++P  + LSLR+LIDHSVVESFG KG+ACIT+RVYP LA
Sbjct: 495 DQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLA 554

Query: 552 IGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           IG +  L+AFN G  SV +  L AWSM  A+I+
Sbjct: 555 IGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of PI0027005 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 776.9 bits (2005), Expect = 1.1e-224
Identity = 381/573 (66.49%), Postives = 443/573 (77.31%), Query Frame = 0

Query: 12  LCFLFGHGVFQLQASHHVYKAQT-SNSLPFHYHQQPYRTSYHFQPPKNWINDPNGPMIYK 71
           L  L G+ V  L+ASHHVYK  T S +       QPYRT +HFQPPKNW+   NGPMIYK
Sbjct: 15  LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM---NGPMIYK 74

Query: 72  GIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPG 131
           GIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AI+PS P DINGCWSGSATILP 
Sbjct: 75  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 134

Query: 132 EKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINASSFRDPT 191
            KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP   N INASSFRDPT
Sbjct: 135 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 194

Query: 192 TAWLGPDGEWRVIVGNKVHTRGLALMYQSKDFVKWDQVDHPLHYADGSGMWECPDFYPVA 251
           TAWLG D +WRVI+G+K+H RGLA+ Y SKDF+KW++   PLHY DGSGMWECPDF+PV 
Sbjct: 195 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 254

Query: 252 KTGRNGVDTI---VNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGSIEGYNGL 311
           + G NGV+T         +KHVLK+SLDDT+HD YTIGTYD  KD +VP+ G        
Sbjct: 255 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 314

Query: 312 RYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWLDASGKQL 371
           RYDYGKYYASKTF+D  K RR+LWGW NESSSVEDD++KGWSGIQ IPR IWLD SGKQL
Sbjct: 315 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 374

Query: 372 IQWPIEEIQKLRKNKV-TLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKAQILKPQ 431
           IQWP+ E+++LR  +V  L NKVLK GS  EV GVT +QADVEV FKV++ +KA +++P 
Sbjct: 375 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 434

Query: 432 YWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFK---KQGKYIVLMCS 491
            W +PQL+CS+      K G+GPFGL+VLAS + +EYTSV F IFK      KY+VLMCS
Sbjct: 435 -WTDPQLICSKMNVSV-KSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCS 494

Query: 492 DQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACITARVYPTLA 551
           DQSRSSL   NDKTTYGAF+D++P  + LSLR+LIDHSVVESFG KG+ACIT+RVYP LA
Sbjct: 495 DQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLA 554

Query: 552 IGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           IG +  L+AFN G  SV +  L AWSM  A+I+
Sbjct: 555 IGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

BLAST of PI0027005 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 724.9 bits (1870), Expect = 5.0e-209
Identity = 348/541 (64.33%), Postives = 413/541 (76.34%), Query Frame = 0

Query: 45  QPYRTSYHFQPPKNWINDPNGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWE 104
           QPYRT YHFQP KNW+NDPNGPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW 
Sbjct: 36  QPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWI 95

Query: 105 PHDHAIYPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLRE 164
               A  PSQPSDINGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLRE
Sbjct: 96  SQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLRE 155

Query: 165 WVKSPKNPLMAPTPQNHINASSFRDPTTAWLGPDGEWRVIVGNKV-HTRGLALMYQSKDF 224
           W K P+NPLM     N IN   FRDPTTAWLG DGEWRVIVG+     RGLA++Y+S+DF
Sbjct: 156 WSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDF 215

Query: 225 VKWDQVDHPLHYADGSGMWECPDFYPVAKTGRNGVDT-IVNRRDVKHVLKVSLDDTRHDV 284
             W Q   PLHY D +GMWECPDF+PV+ TG +GV+T  V    +KHVLKVSL +T HD 
Sbjct: 216 FNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDY 275

Query: 285 YTIGTYDLKKDIYVPNKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVE 344
           YTIG+YD +KD+YVP+ G ++  +  R DYGKYYASKTFYD  KKRR+LWGWVNESS  +
Sbjct: 276 YTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAK 335

Query: 345 DDIKKGWSGIQAIPRSIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGV 404
           DDI+KGWSG+Q+ PR IWLD SGK+L+QWPIEEI+ LR  +V    KVLK GS+ +V GV
Sbjct: 336 DDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGV 395

Query: 405 TPSQADVEVSFKVKNFKKAQILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFK 464
           T +QADVEVSFKVK  +KA +++P  W +PQ +CSQ G  +   G+GPFGL+VLASND +
Sbjct: 396 TAAQADVEVSFKVKELEKADVIEPS-WTDPQKICSQ-GDLSVMSGLGPFGLMVLASNDME 455

Query: 465 EYTSVSFTIFK------KQGKYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLR 524
           EYTSV F IFK      K+ KY+VLMCSDQSRSSLN  NDK+T+GAF+ +DP  + +SLR
Sbjct: 456 EYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLR 515

Query: 525 SLIDHSVVESFGSKGKACITARVYPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARI 577
           +LIDHS+VES+G  G+ CIT+RVYP LAIG+N  L+ FN GT SV I  L+AWS+K A+I
Sbjct: 516 TLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 574

BLAST of PI0027005 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 686.0 bits (1769), Expect = 2.6e-197
Identity = 331/546 (60.62%), Postives = 411/546 (75.27%), Query Frame = 0

Query: 36  NSLPFHYHQQPYRTSYHFQPPKNWINDPNGPMIYKGIYHLFYQYNPKGAVW--GNIVWAH 95
           +++P +   QPYRT YHFQPPKNW+   NGPMIYKGIYHLFYQ+N  GAV      VW H
Sbjct: 31  DNVPNNIKNQPYRTGYHFQPPKNWM---NGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGH 90

Query: 96  STSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVP 155
           +TSTDLINW     AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  P
Sbjct: 91  ATSTDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKP 150

Query: 156 KNLSDPYLREWVKSPKNPLMAPTPQNHINASSFRDPTTAWLGPDGEWRVIVGNKVHTRGL 215
           KNL+DPYLR W KSP+NPL+ P+P NHIN+S+FRDPTTAW G DG WR+  G++   RGL
Sbjct: 151 KNLTDPYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGL 210

Query: 216 ALMYQSKDFVKWDQVDHPLHYADGSGMWECPDFYPVAKTGRNGVDTIVNRRD-VKHVLKV 275
           A+++ SKDFV W Q   PLHY DG+GMWECPDF+PVA+T   G+DT  +    VKHVLKV
Sbjct: 211 AILHTSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKV 270

Query: 276 SLDDTRHDVYTIGTYDLKKDIYVPNKGSIEGYNGLRYDYGKYYASKTFYDGQKKRRVLWG 335
           SL DT +D YTIGTYD  +D+YVP+KG ++     RYDYGK+YASKTFYD   +RR+LWG
Sbjct: 271 SLTDTFNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWG 330

Query: 336 WVNESSSVEDDIKKGWSGIQAIPRSIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKK 395
           WVNESS  +D+IKKGW+G+QAIPR +WLD SGK+L+QWP++EI++LR  +V   NK+LK 
Sbjct: 331 WVNESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKG 390

Query: 396 GSSTEVKGVTPSQADVEVSFKVKNF--KKAQILKPQYWKNPQLLCSQKGTETKKGGVGPF 455
           GS  EV GVT  QADVEV FKV  F  +KA +++P  W +PQL+CSQ+   +   G+GPF
Sbjct: 391 GSVMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPG-WTDPQLICSQRNASS---GLGPF 450

Query: 456 GLLVLASNDFKEYTSVSFTIFK---KQGKYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDP 515
           GL+VLAS + +EYTSV+  IF+      +++V+MCSDQS SSL   NDKTTYGAFLD+ P
Sbjct: 451 GLMVLASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP 510

Query: 516 ISEDLSLRSLIDHSVVESFGSKGKACITARVYPTLAIGDNVGLYAFNNGTDSVTITKLTA 574
             + +SLR+LID S+VESFG KGK CIT+RVYP LAIG+   L+AFN G+ +V +  L+A
Sbjct: 511 Y-QPISLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSA 568

BLAST of PI0027005 vs. TAIR 10
Match: AT1G55120.2 (beta-fructofuranosidase 5 )

HSP 1 Score: 684.9 bits (1766), Expect = 5.7e-197
Identity = 331/521 (63.53%), Postives = 395/521 (75.82%), Query Frame = 0

Query: 65  GPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSG 124
           GPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW     A  PSQPSDINGCWSG
Sbjct: 17  GPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSG 76

Query: 125 SATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINA 184
           S TILP  KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN 
Sbjct: 77  SVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINP 136

Query: 185 SSFRDPTTAWLGPDGEWRVIVGNKV-HTRGLALMYQSKDFVKWDQVDHPLHYADGSGMWE 244
             FRDPTTAWLG DGEWRVIVG+     RGLA++Y+S+DF  W Q   PLHY D +GMWE
Sbjct: 137 DRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWE 196

Query: 245 CPDFYPVAKTGRNGVDT-IVNRRDVKHVLKVSLDDTRHDVYTIGTYDLKKDIYVPNKGSI 304
           CPDF+PV+ TG +GV+T  V    +KHVLKVSL +T HD YTIG+YD +KD+YVP+ G +
Sbjct: 197 CPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFV 256

Query: 305 EGYNGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRSIWLD 364
           +  +  R DYGKYYASKTFYD  KKRR+LWGWVNESS  +DDI+KGWSG+Q+ PR IWLD
Sbjct: 257 QNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLD 316

Query: 365 ASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSSTEVKGVTPSQADVEVSFKVKNFKKAQ 424
            SGK+L+QWPIEEI+ LR  +V    KVLK GS+ +V GVT +QADVEVSFKVK  +KA 
Sbjct: 317 ESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKAD 376

Query: 425 ILKPQYWKNPQLLCSQKGTETKKGGVGPFGLLVLASNDFKEYTSVSFTIFK------KQG 484
           +++P  W +PQ +CSQ G  +   G+GPFGL+VLASND +EYTSV F IFK      K+ 
Sbjct: 377 VIEPS-WTDPQKICSQ-GDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKT 436

Query: 485 KYIVLMCSDQSRSSLNPTNDKTTYGAFLDVDPISEDLSLRSLIDHSVVESFGSKGKACIT 544
           KY+VLMCSDQSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHS+VES+G  G+ CIT
Sbjct: 437 KYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCIT 496

Query: 545 ARVYPTLAIGDNVGLYAFNNGTDSVTITKLTAWSMKKARIN 577
           +RVYP LAIG+N  L+ FN GT SV I  L+AWS+K A+IN
Sbjct: 497 SRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 535

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q438662.3e-22767.02Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... [more]
Q430892.1e-21264.81Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... [more]
Q70XE66.3e-20962.18Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1[more]
Q67XZ37.0e-20864.33Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... [more]
Q9LIB95.4e-20061.17Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0K7610.0e+0093.43Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1[more]
A0A5A7UWG35.1e-29493.40Beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucumis melo var. ma... [more]
A0A6J1GPU76.6e-29484.03beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1GPY11.8e-29183.13beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A2I4JRK21.7e-28982.30Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141685.20.0e+0093.43beta-fructofuranosidase, insoluble isoenzyme CWINV1 [Cucumis sativus] >ALS30216.... [more]
XP_038898203.10.0e+0090.63beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida][more]
KAE8646511.10.0e+0089.85hypothetical protein Csa_015867 [Cucumis sativus][more]
KAA0059424.11.0e-29393.40beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucumis melo var. maku... [more]
XP_022954011.11.4e-29384.03beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >X... [more]
Match NameE-valueIdentityDescription
AT3G13790.11.6e-22867.02Glycosyl hydrolases family 32 protein [more]
AT3G13790.21.1e-22466.49Glycosyl hydrolases family 32 protein [more]
AT1G55120.15.0e-20964.33beta-fructofuranosidase 5 [more]
AT3G13784.12.6e-19760.62cell wall invertase 5 [more]
AT1G55120.25.7e-19763.53beta-fructofuranosidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 52..530
e-value: 5.9E-248
score: 839.7
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 52..371
e-value: 2.2E-108
score: 362.5
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 374..569
e-value: 7.4E-30
score: 104.0
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 56..382
e-value: 2.1E-119
score: 400.9
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 42..372
NoneNo IPR availableGENE3D2.60.120.560coord: 383..570
e-value: 3.2E-77
score: 260.5
NoneNo IPR availablePANTHERPTHR31953:SF51CELL-WALL INVERTASEcoord: 7..576
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 7..576
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 58..360
e-value: 1.68258E-169
score: 481.116
IPR018053Glycoside hydrolase, family 32, active sitePROSITEPS00609GLYCOSYL_HYDROL_F32coord: 52..65
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 371..572

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027005.1PI0027005.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds