PI0026885 (gene) Melon (PI 482460) v1

Overview
NamePI0026885
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Description(1->3)-beta-glucan endohydrolase
Locationchr07: 19305207 .. 19306952 (+)
RNA-Seq ExpressionPI0026885
SyntenyPI0026885
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTTCTTCTTTCCTACTTCCTTTCTTGTGTTACTCTATCTCTTTTCTCATCAAATCCCTCCTATAAGTACTTTTACATGTTGTACCTTTGTTCAATTTCTCTATTCCTTTCTTCCTTCTTTGTTTTGATGGGTTGAGTTTTGTGTTCTAGGTGGCTTCGGCACTGTCGGAGTGAACTATGGGTTGAATGGCGATAATCTTCCAATACCGAATGATGTCATAAACCTATATGGACGATGTGGGATAAATATCGTACGGATATTTGAGCCGAACCATGGGGTTCTTGATGCTTTACAAGGTAAGAAAGATTTAGTGTTGTGGCTAGGGACGAGAAATGAAGACATAGAGGGGTTTGCTACAAACCATTTAGCGGCAAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGGATGTCAACATTGCTTATATCACTATTGGCAATGAAGTTGTCCCTGGTGATACAGCTGCACCTTTTGTTGCCAATGCCATTAAAAATATAATGCAGGCCCTTGTCAATGTTGGCGTTAAAAGCGACATTAAGGTGATGACAATCTATTGAGTTAAATTATTAATTTAGTTTCTATATGAATATTTTATTTGATTGGTATATTATTTGGATTTGATTTTTATAATTCTCAACATTTTCATAAATTAAAAAACAAAGTTTTAGTTTCATTTTGTCCCCTAAAAATTTATAGTTGTAGTTTGTAAATATATATCTAAGTTAGTTTTTCCATATGTAATCCATTTTTCCTAAAGTAACTATATGCATAGTCTTTTAACCTTTATCATTTTAAAATTTTCAATTAGCTTTCTATTTTTAGACTTATTGTCAGCCTAATGTGAGAATGTGTTTATTGGTTTGATTTTAAAAAATGAAAAACAATTTGTTTGCTATAGACATATTAATTATTGAAAATCGATTTTGATGATATAAAATTTACCTTTAAAAGTGTGAAATTAAATGTTAAATTAATTTTGAGTGATTAAAGACGTGTACGGTCAGAATGATTTTAAATATAATAAAAGTTATTTTAATTTAAAATTTTAAAATCATTCCAATCAGACATGTATTAATTAATTAAGACGTTAGAAATCAAATAGCGGAACCGAACTACAACTTATACTTATACATAGTGTGATTTTGGAGTAAATTAAATATGTGCAGGTGACAACAGTGGTATCCATGGCAGCATTGGAAGTGTCATACCCACCTTCAGCGGGAGCATTCACAGATAGTGCTGCTGGAATAATGAAAGACATCGCAAATGTGTTGGGATCTTCGGGGGCACCAATATTGGTGAATGTGTACCCATATTTTGCCTACGCATCGAACCCACAACAAATCTCACTCAATTACGCGCTCTTCACATCCACTACCCCTGTTGTTGTGGATGGAGATTTACAGTACTTCAATCTGTTCGATGCTATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGACGCTGGAGAATTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAATGGAAACGAACCATTCACAAGCGTTGACAATGCATTGACTTATAGCAAAAACCTTGTGAAACATGTGATAAGTGGAGTTGGAACCCCCAAAAGGCCCAATCTCAAATACGATGTTGTTTTGTTCGAAATGTTCAATGAGGATCTTAAAGCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTTATCCTAATATGAACCCAGTTTATCCCTTCTGGAATTGTTGA

mRNA sequence

ATGGACTTCTTCTTTCCTACTTCCTTTCTTGTGTTACTCTATCTCTTTTCTCATCAAATCCCTCCTATAAGTGGCTTCGGCACTGTCGGAGTGAACTATGGGTTGAATGGCGATAATCTTCCAATACCGAATGATGTCATAAACCTATATGGACGATGTGGGATAAATATCGTACGGATATTTGAGCCGAACCATGGGGTTCTTGATGCTTTACAAGGTAAGAAAGATTTAGTGTTGTGGCTAGGGACGAGAAATGAAGACATAGAGGGGTTTGCTACAAACCATTTAGCGGCAAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGGATGTCAACATTGCTTATATCACTATTGGCAATGAAGTTGTCCCTGGTGATACAGCTGCACCTTTTGTTGCCAATGCCATTAAAAATATAATGCAGGCCCTTGTCAATGTTGGCGTTAAAAGCGACATTAAGGTGACAACAGTGGTATCCATGGCAGCATTGGAAGTGTCATACCCACCTTCAGCGGGAGCATTCACAGATAGTGCTGCTGGAATAATGAAAGACATCGCAAATGTGTTGGGATCTTCGGGGGCACCAATATTGGTGAATGTGTACCCATATTTTGCCTACGCATCGAACCCACAACAAATCTCACTCAATTACGCGCTCTTCACATCCACTACCCCTGTTGTTGTGGATGGAGATTTACAGTACTTCAATCTGTTCGATGCTATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGACGCTGGAGAATTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAATGGAAACGAACCATTCACAAGCGTTGACAATGCATTGACTTATAGCAAAAACCTTGTGAAACATGTGATAAGTGGAGTTGGAACCCCCAAAAGGCCCAATCTCAAATACGATGTTGTTTTGTTCGAAATGTTCAATGAGGATCTTAAAGCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTTATCCTAATATGAACCCAGTTTATCCCTTCTGGAATTGTTGA

Coding sequence (CDS)

ATGGACTTCTTCTTTCCTACTTCCTTTCTTGTGTTACTCTATCTCTTTTCTCATCAAATCCCTCCTATAAGTGGCTTCGGCACTGTCGGAGTGAACTATGGGTTGAATGGCGATAATCTTCCAATACCGAATGATGTCATAAACCTATATGGACGATGTGGGATAAATATCGTACGGATATTTGAGCCGAACCATGGGGTTCTTGATGCTTTACAAGGTAAGAAAGATTTAGTGTTGTGGCTAGGGACGAGAAATGAAGACATAGAGGGGTTTGCTACAAACCATTTAGCGGCAAATGCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGGATGTCAACATTGCTTATATCACTATTGGCAATGAAGTTGTCCCTGGTGATACAGCTGCACCTTTTGTTGCCAATGCCATTAAAAATATAATGCAGGCCCTTGTCAATGTTGGCGTTAAAAGCGACATTAAGGTGACAACAGTGGTATCCATGGCAGCATTGGAAGTGTCATACCCACCTTCAGCGGGAGCATTCACAGATAGTGCTGCTGGAATAATGAAAGACATCGCAAATGTGTTGGGATCTTCGGGGGCACCAATATTGGTGAATGTGTACCCATATTTTGCCTACGCATCGAACCCACAACAAATCTCACTCAATTACGCGCTCTTCACATCCACTACCCCTGTTGTTGTGGATGGAGATTTACAGTACTTCAATCTGTTCGATGCTATGGTGGATTCATTCTATGCAGCGCTGGAGAAGATTGACGCTGGAGAATTGAGAATCGGGATATCAGAGACTGGGTGGCCCACCAATGGAAACGAACCATTCACAAGCGTTGACAATGCATTGACTTATAGCAAAAACCTTGTGAAACATGTGATAAGTGGAGTTGGAACCCCCAAAAGGCCCAATCTCAAATACGATGTTGTTTTGTTCGAAATGTTCAATGAGGATCTTAAAGCGCCTGGAGTTGAGCAGAATTTTGGCTTCTTTTATCCTAATATGAACCCAGTTTATCCCTTCTGGAATTGTTGA

Protein sequence

MDFFFPTSFLVLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVYPFWNC
Homology
BLAST of PI0026885 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 7.4e-80
Identity = 147/312 (47.12%), Postives = 206/312 (66.03%), Query Frame = 0

Query: 28  TVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLGTRNED 87
           ++GV YG+ G+NLP P++V++LY    I  +R+++PN   L AL+     VL L     D
Sbjct: 28  SIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVL-LDVPRSD 87

Query: 88  IEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIMQALVN 147
           ++  A+N  AA  W+  NVV Y+  V+  YI +GNE++PG   A ++  A++NI  AL +
Sbjct: 88  VQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSS 147

Query: 148 VGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFA 207
            G+++ IKV+T V    L  SYPPSAGAF+ +A   +  I   L S+GAP+LVNVYPYF+
Sbjct: 148 AGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFS 207

Query: 208 YASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAGELRIGISETG 267
           Y  NP QISL YALFT++  VV DG   Y NLFDA+VD+ +AALE++    + + +SE+G
Sbjct: 208 YTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESG 267

Query: 268 WPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQN 327
           WP+ G     S  NA TY++NL++HV  G GTP+RP  + +  +FEMFNE+ KA G+EQN
Sbjct: 268 WPSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQN 327

Query: 328 FGFFYPNMNPVY 340
           FG FYPN  PVY
Sbjct: 328 FGLFYPNKQPVY 336

BLAST of PI0026885 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.4e-78
Identity = 154/333 (46.25%), Postives = 217/333 (65.17%), Query Frame = 0

Query: 11  VLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDA 70
           ++LY+ S+    ++   ++G+NYGL GDNLP P++VINLY   GI+ +RIF+PN  VL+A
Sbjct: 16  IVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNA 75

Query: 71  LQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTA 130
           L+G +D+ + +G +++D+   A +  A   W  AN+  Y  DVNI +IT+GNEV+PG   
Sbjct: 76  LRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-- 135

Query: 131 APFVANAIKNIMQALVNVGVKS---DIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDI 190
              +   +  +MQ+L N+ VKS    I ++TVV+M+ LE SYPPSAG FT  A   +  +
Sbjct: 136 ---IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPV 195

Query: 191 ANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSF 250
             +L  +  PILVN+YPYFAYAS+P  I L+YA F + + VV DG L Y N+FDA+ D+F
Sbjct: 196 LKLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAF 255

Query: 251 YAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKY 310
             A+EK     L + +SETGWP+ GN  FT+   A TY++N VKH+ SG GTPKRPN   
Sbjct: 256 VWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSM 315

Query: 311 DVVLFEMFNEDLKAPGVEQNFGFFYP-NMNPVY 340
           +  LF  FNE+ K  G EQNFG + P +M P+Y
Sbjct: 316 NGFLFATFNENQKPAGTEQNFGLYNPSDMKPIY 342

BLAST of PI0026885 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 4.9e-76
Identity = 155/347 (44.67%), Postives = 215/347 (61.96%), Query Frame = 0

Query: 3   FFFPTSFLVLLYLFSHQIPPISGFG------TVGVNYGLNGDNLPIPNDVINLYGRCGIN 62
           FFF    +++ Y  S  +   +  G      ++G+NYGL GDNLP P+ VI LY    I 
Sbjct: 11  FFFSCIVVIVNYNNSDFVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDIT 70

Query: 63  IVRIFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIA 122
            +RIF+PN  VL+AL+G +D+ + +G R++D+   + +  A   W   N+ PY  D+NIA
Sbjct: 71  KIRIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIA 130

Query: 123 YITIGNEVVPGDTAAPFVANAIKNIMQALVNVGVKS---DIKVTTVVSMAALEVSYPPSA 182
           +IT+GNEV+PG      +   +  +MQ+L N+ VKS    I ++TVV+M  LE SYPPSA
Sbjct: 131 FITVGNEVIPGP-----IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMWNLEQSYPPSA 190

Query: 183 GAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGD 242
           G FT  A   +  +  +L  + +PILV +YPYF+YAS+P  I L+YA F +   VV DG 
Sbjct: 191 GMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGS 250

Query: 243 LQYFNLFDAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHV 302
           L Y N+FDA+ D+F  A+EK    +L + +SETGWP+ GN   T+ D A TY++N VKH+
Sbjct: 251 LGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHI 310

Query: 303 ISGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPN-MNPVY 340
            SG GTPKRPN   D  LF  FNE+ K  G EQNFG + PN M P+Y
Sbjct: 311 ASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFGLYNPNDMKPIY 351

BLAST of PI0026885 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 144/319 (45.14%), Postives = 204/319 (63.95%), Query Frame = 0

Query: 23  ISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLG 82
           ++G   +GVNYG+ G +LP P+ V+ LY    I  VRIF P+  VL+AL+    L + LG
Sbjct: 1   LAGVEGIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALR-NSGLGVVLG 60

Query: 83  TRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIM 142
           T N D+   A++   A +WV++ V P+   V+  YI  GNEV+PG++AA  V  A+KN+ 
Sbjct: 61  TLNSDLAPLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLE 120

Query: 143 QALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNV 202
            AL   G+   + VTT ++ + L  SYPPS G F+++A   +  I + L SSG P+LVNV
Sbjct: 121 AALQAAGL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNV 180

Query: 203 YPYFAYASNPQQISLNYALFTSTTPV-VVDGDLQYFNLFDAMVDSFYAALEKIDAGE-LR 262
           YPYFAY+++P  + L+YAL +S+  V V D  ++Y N+FDA++D+ YAA+EK   GE L 
Sbjct: 181 YPYFAYSADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLE 240

Query: 263 IGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLK 322
           + +SETGWP+ G     SV+NA  Y  NLV+HV    GTP+RP    +  +F MFNE+ K
Sbjct: 241 LVVSETGWPSGGGGYGASVENAAAYINNLVRHV---GGTPRRPGKAVETYIFAMFNENQK 300

Query: 323 APGVEQNFGFFYPNMNPVY 340
             GVEQNFG F P+M+ VY
Sbjct: 301 PEGVEQNFGMFQPDMSQVY 312

BLAST of PI0026885 vs. ExPASy Swiss-Prot
Match: P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 146/334 (43.71%), Postives = 200/334 (59.88%), Query Frame = 0

Query: 5   FPTSFLVLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPN 64
           FP+   V+L LF            VGV YG+ G+NLP  ++VI LY +  I  +RI++PN
Sbjct: 14  FPSRTTVMLLLFFFAASVGITDAQVGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPN 73

Query: 65  HGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEV 124
             VL+AL+G  ++ L LG  N D++   TN   A +WV  NV  ++  V   YI +GNE+
Sbjct: 74  RAVLEALRG-SNIELILGVPNSDLQSL-TNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEI 133

Query: 125 VPGDTA----APFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSA 184
            P +      A FV  A++NI  A+ + G++  IKV+T + +  +  SYPPSAGAF D  
Sbjct: 134 SPVNRGTAWLAQFVLPAMRNIHDAIRSAGLQDQIKVSTAIDLTLVGNSYPPSAGAFRDDV 193

Query: 185 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 244
              +  I   L S  +P+L N+YPYF YA NP+ ISL YALFTS + VV DG   Y NLF
Sbjct: 194 RSYLDPIIGFLSSIRSPLLANIYPYFTYAYNPRDISLPYALFTSPSVVVWDGQRGYKNLF 253

Query: 245 DAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTP 304
           DA +D+ Y+ALE+   G L + +SE+GWP+ G    T  DN  TY  NL++HV    GTP
Sbjct: 254 DATLDALYSALERASGGSLEVVVSESGWPSAGAFAAT-FDNGRTYLSNLIQHVKG--GTP 313

Query: 305 KRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPN 335
           KRPN   +  LF MF+E+ K P VE++FG F+PN
Sbjct: 314 KRPNRAIETYLFAMFDENKKQPEVEKHFGLFFPN 342

BLAST of PI0026885 vs. ExPASy TrEMBL
Match: A0A5D3D518 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001700 PE=3 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 1.8e-185
Identity = 319/344 (92.73%), Postives = 332/344 (96.51%), Query Frame = 0

Query: 1   MDFFFPTSFLVLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRI 60
           MDFFFPTSFL+LL LFSHQIPPISG GT+GVNYGLNGDNLPIP+DVINLY RCGINIVR+
Sbjct: 1   MDFFFPTSFLLLLCLFSHQIPPISGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRL 60

Query: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITI 120
           FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATN LAAN WVNANVVPYYKDVNI+YIT+
Sbjct: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITV 120

Query: 121 GNEVVPGDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSA 180
           GNEVVPGD AAPFVANAIKNIMQALVN+G+KSDIKVTTVV+M  LEVSYPPSAGAFTDSA
Sbjct: 121 GNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSA 180

Query: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240
           AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF
Sbjct: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240

Query: 241 DAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTP 300
           DAMVDSFYAALEKIDA E+RIGISETGWPT+GNEPFTSVDNALTY+KNLV HV SGVGTP
Sbjct: 241 DAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTP 300

Query: 301 KRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVYPFWNC 345
           KRPNLKYD VLFEMFNEDLKAPGVEQNFGFF+PNMNPVYPFWNC
Sbjct: 301 KRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 344

BLAST of PI0026885 vs. ExPASy TrEMBL
Match: A0A0A0L3A5 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.5e-184
Identity = 319/344 (92.73%), Postives = 334/344 (97.09%), Query Frame = 0

Query: 1   MDFFFPTSFLVLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRI 60
           MDFFFPTSFL+LL LFSHQIPPISGFGT+GVNYGLNGDNLP P+DVINLYGRCGINI+R+
Sbjct: 1   MDFFFPTSFLLLLSLFSHQIPPISGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRL 60

Query: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITI 120
           FEPNHGVLDALQGKKDLVLWLGTRNEDI+GFATN LAANAWVNANVVPYYK+VNIAYITI
Sbjct: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITI 120

Query: 121 GNEVVPGDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSA 180
           GNEVVPGD AAPFVAN IKNIMQALV+VG+KSDIKVTTVV+M AL VSYPPSAGAFT SA
Sbjct: 121 GNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSA 180

Query: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240
           AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTS+TPVVVDG+LQYFNLF
Sbjct: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLF 240

Query: 241 DAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTP 300
           DAMVDSFYAALEKIDAGE+RIGISETGWPTNGNEPFTSV+NALTY+KNLVKHV SGVGTP
Sbjct: 241 DAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTP 300

Query: 301 KRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVYPFWNC 345
           KRPNLKYDVVLFEMFNEDLKAPGVEQNFGFF PNMNPVYPFWNC
Sbjct: 301 KRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFSPNMNPVYPFWNC 344

BLAST of PI0026885 vs. ExPASy TrEMBL
Match: A0A5A7V7B6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G002240 PE=3 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 1.0e-172
Identity = 297/320 (92.81%), Postives = 309/320 (96.56%), Query Frame = 0

Query: 25  GFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLGTR 84
           G GT+GVNYGLNGDNLPIP+DVINLY RCGINIVR+FEPNHGVLDALQGKKDLVLWLGTR
Sbjct: 345 GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 404

Query: 85  NEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIMQA 144
           NEDIEGFATN LAAN WVNANVVPYYKDVNI+YIT+GNEVVPGD AAPFVANAIKNIMQA
Sbjct: 405 NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 464

Query: 145 LVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 204
           LVN+G+KSDIKVTTVV+M  LEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP
Sbjct: 465 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 524

Query: 205 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAGELRIGIS 264
           YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDA E+RIGIS
Sbjct: 525 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 584

Query: 265 ETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLKAPGV 324
           ETGWPT+GNEPFTSVDNALTY+KNLV HV SGVGTPKRPNLKYD VLFEMFNEDLKAPGV
Sbjct: 585 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 644

Query: 325 EQNFGFFYPNMNPVYPFWNC 345
           EQNFGFF+PNMNPVYPFWNC
Sbjct: 645 EQNFGFFFPNMNPVYPFWNC 664

BLAST of PI0026885 vs. ExPASy TrEMBL
Match: A0A1S4DV56 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 303/343 (88.34%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 4   FFPTSFLVLLYLFS--HQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIF 63
           FF   F +L   F+  + + P  G GT+GVNYGLNGDNLPIP+DVINLY RCGINIVR+F
Sbjct: 311 FFSIPFFLLFLCFNVLNFLFP-GGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLF 370

Query: 64  EPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIG 123
           EPNHGVLDALQGKKDLVLWLGTRNEDIEGFATN LAAN WVNANVVPYYKDVNI+YIT+G
Sbjct: 371 EPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVG 430

Query: 124 NEVVPGDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAA 183
           NEVVPGD AAPFVANAIKNIMQALVN+G+KSDIKVTTVV+M  LEVSYPPSAGAFTDSAA
Sbjct: 431 NEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAA 490

Query: 184 GIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFD 243
           GIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFD
Sbjct: 491 GIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFD 550

Query: 244 AMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPK 303
           AMVDSFYAALEKIDA E+RIGISETGWPT+GNEPFTSVDNALTY+KNLV HV SGVGTPK
Sbjct: 551 AMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPK 610

Query: 304 RPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVYPFWNC 345
           RPNLKYD VLFEMFNEDLKAPGVEQNFGFF+PNMNPVYPFWNC
Sbjct: 611 RPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 652

BLAST of PI0026885 vs. ExPASy TrEMBL
Match: A0A5D3D504 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001690 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.8e-156
Identity = 277/344 (80.52%), Postives = 304/344 (88.37%), Query Frame = 0

Query: 1   MDFFFPTSFLVLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRI 60
           M  FFPT+ L+L+++FSHQI  IS FG VGVNYGLN DNLP P++VINLY RCGINIVRI
Sbjct: 1   MALFFPTTSLLLIFIFSHQILHISVFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRI 60

Query: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITI 120
           FEPNH VLDAL+ K++LVLWLGTRNEDI+GFATN   A+AWVNANVVPYYKDVNIAYIT+
Sbjct: 61  FEPNHEVLDALRCKENLVLWLGTRNEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITV 120

Query: 121 GNEVVPGDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSA 180
           GNEVVPGD A PFVANAI+NIMQALVN  V+SDIKVTTVV+M ALEVS PPSAGAFT  A
Sbjct: 121 GNEVVPGDAATPFVANAIQNIMQALVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIA 180

Query: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240
           A  MKDI +VL SS APIL+NVYPYFAYASNPQQI L+YALFTSTTPVVVDGDL+YFNL 
Sbjct: 181 ARAMKDIGSVLESSCAPILMNVYPYFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLL 240

Query: 241 DAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTP 300
           DAMVDSFY ALEKI    +RIGISETGWP+ GNEPFTSVDNALTY++N VKHV SGVGTP
Sbjct: 241 DAMVDSFYTALEKIGVEGVRIGISETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTP 300

Query: 301 KRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVYPFWNC 345
           K PNL+YD+VLFEMFNEDLK+PGVEQNFGFFYPNMNPVY FWNC
Sbjct: 301 KMPNLQYDIVLFEMFNEDLKSPGVEQNFGFFYPNMNPVYSFWNC 344

BLAST of PI0026885 vs. NCBI nr
Match: TYK18632.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 658.3 bits (1697), Expect = 3.7e-185
Identity = 319/344 (92.73%), Postives = 332/344 (96.51%), Query Frame = 0

Query: 1   MDFFFPTSFLVLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRI 60
           MDFFFPTSFL+LL LFSHQIPPISG GT+GVNYGLNGDNLPIP+DVINLY RCGINIVR+
Sbjct: 1   MDFFFPTSFLLLLCLFSHQIPPISGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRL 60

Query: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITI 120
           FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATN LAAN WVNANVVPYYKDVNI+YIT+
Sbjct: 61  FEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITV 120

Query: 121 GNEVVPGDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSA 180
           GNEVVPGD AAPFVANAIKNIMQALVN+G+KSDIKVTTVV+M  LEVSYPPSAGAFTDSA
Sbjct: 121 GNEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSA 180

Query: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240
           AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF
Sbjct: 181 AGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLF 240

Query: 241 DAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTP 300
           DAMVDSFYAALEKIDA E+RIGISETGWPT+GNEPFTSVDNALTY+KNLV HV SGVGTP
Sbjct: 241 DAMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTP 300

Query: 301 KRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVYPFWNC 345
           KRPNLKYD VLFEMFNEDLKAPGVEQNFGFF+PNMNPVYPFWNC
Sbjct: 301 KRPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 344

BLAST of PI0026885 vs. NCBI nr
Match: KAA0064262.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 615.9 bits (1587), Expect = 2.1e-172
Identity = 297/320 (92.81%), Postives = 309/320 (96.56%), Query Frame = 0

Query: 25  GFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLGTR 84
           G GT+GVNYGLNGDNLPIP+DVINLY RCGINIVR+FEPNHGVLDALQGKKDLVLWLGTR
Sbjct: 345 GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 404

Query: 85  NEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIMQA 144
           NEDIEGFATN LAAN WVNANVVPYYKDVNI+YIT+GNEVVPGD AAPFVANAIKNIMQA
Sbjct: 405 NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 464

Query: 145 LVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 204
           LVN+G+KSDIKVTTVV+M  LEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP
Sbjct: 465 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 524

Query: 205 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAGELRIGIS 264
           YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDA E+RIGIS
Sbjct: 525 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 584

Query: 265 ETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLKAPGV 324
           ETGWPT+GNEPFTSVDNALTY+KNLV HV SGVGTPKRPNLKYD VLFEMFNEDLKAPGV
Sbjct: 585 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 644

Query: 325 EQNFGFFYPNMNPVYPFWNC 345
           EQNFGFF+PNMNPVYPFWNC
Sbjct: 645 EQNFGFFFPNMNPVYPFWNC 664

BLAST of PI0026885 vs. NCBI nr
Match: XP_016899853.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo])

HSP 1 Score: 615.5 bits (1586), Expect = 2.7e-172
Identity = 303/343 (88.34%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 4   FFPTSFLVLLYLFS--HQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIF 63
           FF   F +L   F+  + + P  G GT+GVNYGLNGDNLPIP+DVINLY RCGINIVR+F
Sbjct: 311 FFSIPFFLLFLCFNVLNFLFP-GGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLF 370

Query: 64  EPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIG 123
           EPNHGVLDALQGKKDLVLWLGTRNEDIEGFATN LAAN WVNANVVPYYKDVNI+YIT+G
Sbjct: 371 EPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVG 430

Query: 124 NEVVPGDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAA 183
           NEVVPGD AAPFVANAIKNIMQALVN+G+KSDIKVTTVV+M  LEVSYPPSAGAFTDSAA
Sbjct: 431 NEVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAA 490

Query: 184 GIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFD 243
           GIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFD
Sbjct: 491 GIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFD 550

Query: 244 AMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPK 303
           AMVDSFYAALEKIDA E+RIGISETGWPT+GNEPFTSVDNALTY+KNLV HV SGVGTPK
Sbjct: 551 AMVDSFYAALEKIDAEEIRIGISETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPK 610

Query: 304 RPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVYPFWNC 345
           RPNLKYD VLFEMFNEDLKAPGVEQNFGFF+PNMNPVYPFWNC
Sbjct: 611 RPNLKYDAVLFEMFNEDLKAPGVEQNFGFFFPNMNPVYPFWNC 652

BLAST of PI0026885 vs. NCBI nr
Match: KAE8649635.1 (hypothetical protein Csa_012736 [Cucumis sativus])

HSP 1 Score: 614.8 bits (1584), Expect = 4.6e-172
Identity = 298/321 (92.83%), Postives = 312/321 (97.20%), Query Frame = 0

Query: 24  SGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLGT 83
           SGFGT+GVNYGLNGDNLP P+DVINLYGRCGINI+R+FEPNHGVLDALQGKKDLVLWLGT
Sbjct: 334 SGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGT 393

Query: 84  RNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIMQ 143
           RNEDI+GFATN LAANAWVNANVVPYYK+VNIAYITIGNEVVPGD AAPFVAN IKNIMQ
Sbjct: 394 RNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQ 453

Query: 144 ALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVY 203
           ALV+VG+KSDIKVTTVV+M AL VSYPPSAGAFT SAAGIMKDIANVLGSSGAPILVNVY
Sbjct: 454 ALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVY 513

Query: 204 PYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAGELRIGI 263
           PYFAYASNPQQISLNYALFTS+TPVVVDG+LQYFNLFDAMVDSFYAALEKIDAGE+RIGI
Sbjct: 514 PYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGI 573

Query: 264 SETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLKAPG 323
           SETGWPTNGNEPFTSV+NALTY+KNLVKHV SGVGTPKRPNLKYDVVLFEMFNEDLKAPG
Sbjct: 574 SETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPG 633

Query: 324 VEQNFGFFYPNMNPVYPFWNC 345
           VEQNFGFF PNMNPVYPFWNC
Sbjct: 634 VEQNFGFFSPNMNPVYPFWNC 654

BLAST of PI0026885 vs. NCBI nr
Match: XP_031740438.1 (glucan endo-1,3-beta-glucosidase-like [Cucumis sativus])

HSP 1 Score: 612.8 bits (1579), Expect = 1.8e-171
Identity = 297/320 (92.81%), Postives = 311/320 (97.19%), Query Frame = 0

Query: 25  GFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLGTR 84
           GFGT+GVNYGLNGDNLP P+DVINLYGRCGINI+R+FEPNHGVLDALQGKKDLVLWLGTR
Sbjct: 5   GFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTR 64

Query: 85  NEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIMQA 144
           NEDI+GFATN LAANAWVNANVVPYYK+VNIAYITIGNEVVPGD AAPFVAN IKNIMQA
Sbjct: 65  NEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQA 124

Query: 145 LVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 204
           LV+VG+KSDIKVTTVV+M AL VSYPPSAGAFT SAAGIMKDIANVLGSSGAPILVNVYP
Sbjct: 125 LVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYP 184

Query: 205 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAGELRIGIS 264
           YFAYASNPQQISLNYALFTS+TPVVVDG+LQYFNLFDAMVDSFYAALEKIDAGE+RIGIS
Sbjct: 185 YFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGIS 244

Query: 265 ETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLKAPGV 324
           ETGWPTNGNEPFTSV+NALTY+KNLVKHV SGVGTPKRPNLKYDVVLFEMFNEDLKAPGV
Sbjct: 245 ETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGV 304

Query: 325 EQNFGFFYPNMNPVYPFWNC 345
           EQNFGFF PNMNPVYPFWNC
Sbjct: 305 EQNFGFFSPNMNPVYPFWNC 324

BLAST of PI0026885 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 5.6e-83
Identity = 155/314 (49.36%), Postives = 213/314 (67.83%), Query Frame = 0

Query: 30  GVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDALQGKKDLVLWLGTRNEDIE 89
           GV YG NGDNLP P   ++LY +  +  +R++EP   ++ +LQG   L++ +G RNE I+
Sbjct: 25  GVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQG-TGLLVAIGPRNEAIK 84

Query: 90  GFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTAAPFVANAIKNIMQALVNVG 149
             A  +  A  W    + P YK+V   +IT+GNEV+ G+    +V  A+KNI  AL  +G
Sbjct: 85  TLAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGEIGR-YVPQAMKNIKAALTEIG 144

Query: 150 VKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYA 209
             S I VTTV+S AAL  SYPPSAG F  +   ++ +I ++L S+ +P++VNVYPYFAYA
Sbjct: 145 -NSKIHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLMVNVYPYFAYA 204

Query: 210 SNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAGELRIGISETGWP 269
           S+P  +SL YA F ST+PVV DG  QY N+FDA +D+F  ALEKI+ G +++ ++ETGWP
Sbjct: 205 SDPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSVKVYVAETGWP 264

Query: 270 TNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFG 329
           T GN+P+TSV+NA  Y++ L+K + +G GTP+RPN+      FEMFNEDLK   VEQ+FG
Sbjct: 265 TRGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDLKQGAVEQSFG 324

Query: 330 FFYPNMNPVYPFWN 344
           FF PNM PVY  WN
Sbjct: 325 FFDPNMAPVYDMWN 334

BLAST of PI0026885 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 293.9 bits (751), Expect = 1.7e-79
Identity = 154/333 (46.25%), Postives = 217/333 (65.17%), Query Frame = 0

Query: 11  VLLYLFSHQIPPISGFGTVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHGVLDA 70
           ++LY+ S+    ++   ++G+NYGL GDNLP P++VINLY   GI+ +RIF+PN  VL+A
Sbjct: 16  IVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNA 75

Query: 71  LQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVPGDTA 130
           L+G +D+ + +G +++D+   A +  A   W  AN+  Y  DVNI +IT+GNEV+PG   
Sbjct: 76  LRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-- 135

Query: 131 APFVANAIKNIMQALVNVGVKS---DIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKDI 190
              +   +  +MQ+L N+ VKS    I ++TVV+M+ LE SYPPSAG FT  A   +  +
Sbjct: 136 ---IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPV 195

Query: 191 ANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSF 250
             +L  +  PILVN+YPYFAYAS+P  I L+YA F + + VV DG L Y N+FDA+ D+F
Sbjct: 196 LKLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAF 255

Query: 251 YAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVISGVGTPKRPNLKY 310
             A+EK     L + +SETGWP+ GN  FT+   A TY++N VKH+ SG GTPKRPN   
Sbjct: 256 VWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSM 315

Query: 311 DVVLFEMFNEDLKAPGVEQNFGFFYP-NMNPVY 340
           +  LF  FNE+ K  G EQNFG + P +M P+Y
Sbjct: 316 NGFLFATFNENQKPAGTEQNFGLYNPSDMKPIY 342

BLAST of PI0026885 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 9.2e-78
Identity = 163/335 (48.66%), Postives = 213/335 (63.58%), Query Frame = 0

Query: 9   FLVLLYLFSHQIPPISGFG--TVGVNYGLNGDNLPIPNDVINLYGRCGINIVRIFEPNHG 68
           F V++ L S QI   +G      G  YG NG+NLP P D + LY    I+ +R++EP   
Sbjct: 8   FAVVIMLLSIQIFCTAGVAGDITGDCYGRNGNNLPTPADTVALYKSNNIDAIRMYEPFAD 67

Query: 69  VLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYITIGNEVVP 128
           +L+AL+G   L++  G RNEDI+  A +  AA  +V+  + PY  DV I +ITIGNEV P
Sbjct: 68  MLEALRG-SGLLVAFGPRNEDIQSLAHDPAAATNFVSTWITPYQNDVAIKWITIGNEVFP 127

Query: 129 GDTAAPFVANAIKNIMQALVNVGVKSDIKVTTVVSMAALEVSYPPSAGAFTDSAAGIMKD 188
           G+  A FVA AIKN+  AL N GV + I VTTV++M AL  +YPPSA  F      IM +
Sbjct: 128 GE-IAQFVAAAIKNVNVALTNSGV-TGISVTTVLAMTALTNTYPPSAATFLPDLTEIMTE 187

Query: 189 IANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDS 248
           I ++L  + +P++ N+YPYFAYAS+P  ISL+YA F S TPVV+DGDL Y N+F+AMVD 
Sbjct: 188 ITSILSETNSPLMTNIYPYFAYASDPYHISLDYASFKSNTPVVIDGDLYYNNMFEAMVDG 247

Query: 249 FYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNL--VKHVISGVGTPKRPN 308
           F AALEKI+A  + + ++ETGWPT GN P TSVDNA  Y+  +           TP+R N
Sbjct: 248 FNAALEKINAANVVVMVAETGWPTEGNPPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQN 307

Query: 309 LKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVY 340
              DV LF MF E+ K   VEQ+FG F P+M PVY
Sbjct: 308 TPVDVFLFAMFKENQKDGPVEQSFGIFAPDMTPVY 339

BLAST of PI0026885 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 286.2 bits (731), Expect = 3.5e-77
Identity = 155/347 (44.67%), Postives = 215/347 (61.96%), Query Frame = 0

Query: 3   FFFPTSFLVLLYLFSHQIPPISGFG------TVGVNYGLNGDNLPIPNDVINLYGRCGIN 62
           FFF    +++ Y  S  +   +  G      ++G+NYGL GDNLP P+ VI LY    I 
Sbjct: 11  FFFSCIVVIVNYNNSDFVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDIT 70

Query: 63  IVRIFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIA 122
            +RIF+PN  VL+AL+G +D+ + +G R++D+   + +  A   W   N+ PY  D+NIA
Sbjct: 71  KIRIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIA 130

Query: 123 YITIGNEVVPGDTAAPFVANAIKNIMQALVNVGVKS---DIKVTTVVSMAALEVSYPPSA 182
           +IT+GNEV+PG      +   +  +MQ+L N+ VKS    I ++TVV+M  LE SYPPSA
Sbjct: 131 FITVGNEVIPGP-----IGPQVLPVMQSLTNL-VKSRNLPISISTVVAMWNLEQSYPPSA 190

Query: 183 GAFTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGD 242
           G FT  A   +  +  +L  + +PILV +YPYF+YAS+P  I L+YA F +   VV DG 
Sbjct: 191 GMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGS 250

Query: 243 LQYFNLFDAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHV 302
           L Y N+FDA+ D+F  A+EK    +L + +SETGWP+ GN   T+ D A TY++N VKH+
Sbjct: 251 LGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHI 310

Query: 303 ISGVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPN-MNPVY 340
            SG GTPKRPN   D  LF  FNE+ K  G EQNFG + PN M P+Y
Sbjct: 311 ASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFGLYNPNDMKPIY 351

BLAST of PI0026885 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.4e-72
Identity = 149/344 (43.31%), Postives = 210/344 (61.05%), Query Frame = 0

Query: 3   FFFPTSFLVLLYLFSHQIPPISGF----GTVGVNYGLNGDNLPIPNDVINLYGRCGINIV 62
           F F  S ++L + ++      SGF     ++G+NYGL GDN P P++V+NLY   GI  +
Sbjct: 9   FLFFLSCIMLTFNYN-----TSGFVAAANSIGLNYGLLGDNFPTPSNVLNLYKSIGITKI 68

Query: 63  RIFEPNHGVLDALQGKKDLVLWLGTRNEDIEGFATNHLAANAWVNANVVPYYKDVNIAYI 122
           RIF+P   VL+AL+G +++ + +G R++D+   + N  A   W   N+ PY  DVNIA+I
Sbjct: 69  RIFDPKTEVLNALRGHRNIEVTVGVRDQDLGALSANEEAVKGWFATNIEPYLADVNIAFI 128

Query: 123 TIGNEVVPGDTAAPFVANAIKNIMQALVNVGVKS---DIKVTTVVSMAALEVSYPPSAGA 182
           T+GNEV+PG      +   +  +MQ+L  + VKS    I ++TVV+M+ LE SYPPSAG 
Sbjct: 129 TVGNEVIPGP-----IGPQVLPVMQSL-TILVKSMNLPISISTVVAMSNLEQSYPPSAGE 188

Query: 183 FTDSAAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSTTPVVVDGDLQ 242
           FT  A   +  +  +L  +  PILVN+YPYF YAS+P  I L+YA F +   VV DG L 
Sbjct: 189 FTSQAREQLVPVLKLLSQTSTPILVNIYPYFPYASDPTNIPLDYATFNTKATVVQDGPLG 248

Query: 243 YFNLFDAMVDSFYAALEKIDAGELRIGISETGWPTNGNEPFTSVDNALTYSKNLVKHVIS 302
           Y N+FDA+ D+F  A+EK    +L + +SETGWP+ GN   T+ D A TY++N VKH++S
Sbjct: 249 YSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPSAGNGNLTTPDIAGTYNRNFVKHIVS 308

Query: 303 GVGTPKRPNLKYDVVLFEMFNEDLKAPGVEQNFGFFYPNMNPVY 340
           G GTPKRPN   D  LF  FNE+ K  G +         M P+Y
Sbjct: 309 GKGTPKRPNNGMDGFLFATFNENQKPAGTD---------MKPIY 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O223177.4e-8047.12Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2[more]
O493522.4e-7846.25Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN... [more]
O493534.9e-7644.67Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q024395.0e-6845.14Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4... [more]
P524071.4e-6743.71Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O... [more]
Match NameE-valueIdentityDescription
A0A5D3D5181.8e-18592.73(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0L3A51.5e-18492.73(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3... [more]
A0A5A7V7B61.0e-17292.81(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S4DV561.3e-17288.34(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV... [more]
A0A5D3D5043.8e-15680.52(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
TYK18632.13.7e-18592.73putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa][more]
KAA0064262.12.1e-17292.81putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa][more]
XP_016899853.12.7e-17288.34PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo][more]
KAE8649635.14.6e-17292.83hypothetical protein Csa_012736 [Cucumis sativus][more]
XP_031740438.11.8e-17192.81glucan endo-1,3-beta-glucosidase-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G77780.15.6e-8349.36Glycosyl hydrolase superfamily protein [more]
AT5G20330.11.7e-7946.25beta-1,3-glucanase 4 [more]
AT1G77790.19.2e-7848.66Glycosyl hydrolase superfamily protein [more]
AT5G20340.13.5e-7744.67beta-1,3-glucanase 5 [more]
AT1G33220.14.4e-7243.31Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..339
e-value: 6.0E-88
score: 295.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..342
e-value: 1.2E-113
score: 381.3
NoneNo IPR availablePANTHERPTHR32227:SF248GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 16..341
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 16..341
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0026885.1PI0026885.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds