PI0026882 (gene) Melon (PI 482460) v1

BLAST of PI0026882 vs. ExPASy Swiss-Prot
Match: Q96DG6 (Carboxymethylenebutenolidase homolog OS=Homo sapiens OX=9606 GN=CMBL PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.9e-13
Identity = 62/219 (28.31%), Postives = 104/219 (47.49%), Query Frame = 0

Query: 74  IPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPD-LVHEDPWEKP 133
           I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD  V ++PW+ P
Sbjct: 30  IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWD-P 89

Query: 134 PAQHSAKQE------MQRIAEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGGDACFLI- 193
               S   E       Q+I  +I  + K+L  +  A     K+GI+GF +GG     L+ 
Sbjct: 90  SGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGTAVHHLMM 149

Query: 194 -------GASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSK---- 253
                  G S+YG   D      +K P L I  +ND + P+  +  L + + E  K    
Sbjct: 150 KYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQ 209

Query: 254 LVIFGGRGHGFVHNPG---SPEEDDDAEQAFLMIRNWLH 271
           +  F G+ HGFVH      SP +    ++A   +  WL+
Sbjct: 210 IKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of PI0026882 vs. ExPASy Swiss-Prot
Match: Q5RBU3 (Carboxymethylenebutenolidase homolog OS=Pongo abelii OX=9601 GN=CMBL PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 8.4e-13
Identity = 62/219 (28.31%), Postives = 104/219 (47.49%), Query Frame = 0

Query: 74  IPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPD-LVHEDPWEKP 133
           I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD  V ++PW+ P
Sbjct: 30  IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYMADMISGNGYTTIVPDFFVGQEPWD-P 89

Query: 134 PAQHSAKQE------MQRIAEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGG-------- 193
               S   E       Q+I  +I  + K+L  +  A     K+GI+GF +GG        
Sbjct: 90  SGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGIAVHHLMM 149

Query: 194 GDACFLIGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSK---- 253
             + F  G S+YG   D      +K P L I  +ND + P+  +  L + + E  K    
Sbjct: 150 KYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQ 209

Query: 254 LVIFGGRGHGFVHNPG---SPEEDDDAEQAFLMIRNWLH 271
           +  F G+ HGFVH      SP +    ++A   +  WL+
Sbjct: 210 IKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of PI0026882 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.2e-12
Identity = 62/246 (25.20%), Postives = 111/246 (45.12%), Query Frame = 0

Query: 51  DDDDACELVNGMELSIGEQPH----HSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRD 110
           ++ + C    G +L  G   H      I AY+ ++   + G A++++ D+F +Q  + R 
Sbjct: 3   NEANPCPCDIGHKLEYGGMGHEVQVEHIKAYVTRS-PVDAGKAVIVVQDIFGWQLPNTRY 62

Query: 111 FAYRLACNGFNVLLPD-LVHEDPWEKPPAQHSA------KQEMQRIAEDIGTVTKWLTDE 170
            A  +A NG+  ++PD  V ++PW+ P    S        +  +++  ++  V ++L  +
Sbjct: 63  MADMIARNGYTTIVPDFFVGQEPWD-PAGDWSTFPAWLKSRNARKVNREVDAVLRYLRQQ 122

Query: 171 FSAAGLSTKLGIMGFGYGG--------GDACFLIGASLYGTEVDPSLATKVKVPVLLISG 230
             A     K+GI+GF +GG               G S+YG   D      +K P L I  
Sbjct: 123 CHA----QKIGIVGFCWGGVVVHQVMTAYPDIRAGVSVYGIIRDSEDVYNLKNPTLFIFA 182

Query: 231 DNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFVHNPG---SPEEDDDAEQAFLM 271
           +ND + P+  +  L + + E      ++  F G+ HGFVH      SP +    E+A   
Sbjct: 183 ENDTVIPLEQVSTLTQKLKEHCIVNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEARRN 242

BLAST of PI0026882 vs. ExPASy Swiss-Prot
Match: Q7TP52 (Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 7.1e-12
Identity = 56/206 (27.18%), Postives = 98/206 (47.57%), Query Frame = 0

Query: 87  GTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPD-LVHEDPWEKPPAQHSA------K 146
           G A++++ D+F +Q S+ R  A  +A NG+  ++PD  V ++PW+ P    S        
Sbjct: 42  GKAVIVVQDIFGWQLSNTRYMADMIAGNGYTTIVPDFFVGQEPWD-PAGDWSTFPEWLKS 101

Query: 147 QEMQRIAEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGGDACFLI--------GASLYG 206
           +  ++I  ++  V ++L  +  A     K+GI+GF +GG     ++        G S+YG
Sbjct: 102 RNARKINREVDAVLRYLKQQCHA----QKIGIVGFCWGGIVVHHVMTTYPEVRAGVSVYG 161

Query: 207 TEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFVH 266
              D      +K P L I  +ND + P+  +  L + + E      ++  F G+ HGFVH
Sbjct: 162 IIRDSEDVYNLKNPTLFIFAENDAVIPLEQVSILIQKLKEHCIVNYQVKTFSGQTHGFVH 221

Query: 267 NPG---SPEEDDDAEQAFLMIRNWLH 271
                 SP +    E+A   +  WL+
Sbjct: 222 RKREDCSPADKPYIEEARRNLIEWLN 242

BLAST of PI0026882 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.3e-138
Identity = 245/269 (91.08%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 27  KYNNRGVWKWKGKLR-YSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 86
           KYNN G WKWKGKLR YSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN
Sbjct: 42  KYNNWGDWKWKGKLRYYSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 101

Query: 87  NGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRI 146
           NGTAILLLSDLFAFQ SS+RDFAYRLACNGFNVLLPDLV+EDPWEKPPAQHSAKQEMQRI
Sbjct: 102 NGTAILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRI 161

Query: 147 AEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGG----------DACFLIGASLYGTEVD 206
           AEDIGTVTKWLTDEFSAAGLS KLGI+GFG+GGG           ACFLIGASLYGTEVD
Sbjct: 162 AEDIGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVD 221

Query: 207 PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEED 266
           PSLATKVKVPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVHNPGSPEED
Sbjct: 222 PSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEED 281

Query: 267 DDAEQAFLMIRNWLHHGLVATNDLANCHG 285
           DDAEQAFLM+RNWLHHGLVATN+LANCHG
Sbjct: 282 DDAEQAFLMLRNWLHHGLVATNNLANCHG 310

BLAST of PI0026882 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.3e-138
Identity = 245/269 (91.08%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 27  KYNNRGVWKWKGKLR-YSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 86
           KYNN G WKWKGKLR YSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN
Sbjct: 42  KYNNWGDWKWKGKLRYYSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 101

Query: 87  NGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRI 146
           NGTAILLLSDLFAFQ SS+RDFAYRLACNGFNVLLPDLV+EDPWEKPPAQHSAKQEMQRI
Sbjct: 102 NGTAILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRI 161

Query: 147 AEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGG----------DACFLIGASLYGTEVD 206
           AEDIGTVTKWLTDEFSAAGLS KLGI+GFG+GGG           ACFLIGASLYGTEVD
Sbjct: 162 AEDIGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVD 221

Query: 207 PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEED 266
           PSLATKVKVPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVHNPGSPEED
Sbjct: 222 PSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEED 281

Query: 267 DDAEQAFLMIRNWLHHGLVATNDLANCHG 285
           DDAEQAFLM+RNWLHHGLVATN+LANCHG
Sbjct: 282 DDAEQAFLMLRNWLHHGLVATNNLANCHG 310

BLAST of PI0026882 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 1.8e-135
Identity = 236/269 (87.73%), Postives = 250/269 (92.94%), Query Frame = 0

Query: 27  KYNNRGVWKWKGKLRYSVKGRVK-DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 86
           KY+N GVW+WKGKLRYSV+GR+K DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN
Sbjct: 42  KYSNMGVWRWKGKLRYSVEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 101

Query: 87  NGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRI 146
           NGTAILLLSDLFAFQHSS+RDFAYRLAC GFNVLLPDLVHED WEKPPA+H AKQEMQRI
Sbjct: 102 NGTAILLLSDLFAFQHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRI 161

Query: 147 AEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGG----------DACFLIGASLYGTEVD 206
           AEDIGTVTKWLT+EFSAAGLS KLGI+GFGYGGG            CFLIGAS+YGT+VD
Sbjct: 162 AEDIGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVD 221

Query: 207 PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEED 266
           PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVHNPGSPEED
Sbjct: 222 PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEED 281

Query: 267 DDAEQAFLMIRNWLHHGLVATNDLANCHG 285
           DDAE+AFLM+RNWLHHGLVA N+LANCHG
Sbjct: 282 DDAEKAFLMLRNWLHHGLVAINNLANCHG 310

BLAST of PI0026882 vs. ExPASy TrEMBL
Match: A0A6J1DNR6 (hydrolase tropI OS=Momordica charantia OX=3673 GN=LOC111022362 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 3.0e-106
Identity = 195/283 (68.90%), Postives = 225/283 (79.51%), Query Frame = 0

Query: 11  VHISTPLMITSFELYEKYNNRGVWKWKGKL-----RYSVKGRVKDDDDDACELVNGMELS 70
           +HI TP       L+ +  N GVWKWK K      +     R++ + D+ CELV+GMELS
Sbjct: 14  LHIGTPF---PSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEGCELVSGMELS 73

Query: 71  IGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVH 130
           IGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAFQ SS+ DFAYR+ACNGFNVLLPD+ H
Sbjct: 74  IGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFH 133

Query: 131 EDPW-EKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGGD---- 190
            DPW +K P + +AKQ+MQR+AEDIGT TKWL +EFSAAG+STKLGIMGFGYGGG     
Sbjct: 134 GDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDV 193

Query: 191 ------ACFLIGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSK 250
                 ACF IGAS YGT +DPSL ++VKVPVLLISGDNDPLCPVSVMKDLEKG+G+GSK
Sbjct: 194 LARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGKGSK 253

Query: 251 LVIFGGRGHGFVHNPGSPEEDDDAEQAFLMIRNWLHHGLVATN 278
           LVIFGGRGHGF H PGSPEED+DAE+AFLM+RNWLH GLV TN
Sbjct: 254 LVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTN 293

BLAST of PI0026882 vs. ExPASy TrEMBL
Match: A0A6J1EC40 (carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111432716 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.3e-98
Identity = 189/252 (75.00%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 38  GKLRYSVKGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 97
           GK+ YS +G VKD  ++++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+D
Sbjct: 53  GKVCYSAEG-VKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTD 112

Query: 98  LFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRIAEDIGTVTKW 157
           LFAFQ SSVRDFAYRLACNGFNVLLPD+ H D  E+           Q+  EDI   TKW
Sbjct: 113 LFAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKW 172

Query: 158 LTDEFSAAGLSTKLGIMGFGYGGG----------DACFLIGASLYGTEVDPSLATKVKVP 217
           L DEFSAAG+S KLGI+GFGYGGG           + F IGASLYGTEV+PSLATKV+VP
Sbjct: 173 LVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVP 232

Query: 218 VLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEQAFLMI 277
           VLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVH PGSPEED+DAEQAFLM+
Sbjct: 233 VLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLML 292

BLAST of PI0026882 vs. NCBI nr
Match: XP_008459689.1 (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa] >TYK00404.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 501.5 bits (1290), Expect = 4.7e-138
Identity = 245/269 (91.08%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 27  KYNNRGVWKWKGKLR-YSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 86
           KYNN G WKWKGKLR YSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN
Sbjct: 42  KYNNWGDWKWKGKLRYYSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 101

Query: 87  NGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRI 146
           NGTAILLLSDLFAFQ SS+RDFAYRLACNGFNVLLPDLV+EDPWEKPPAQHSAKQEMQRI
Sbjct: 102 NGTAILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRI 161

Query: 147 AEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGG----------DACFLIGASLYGTEVD 206
           AEDIGTVTKWLTDEFSAAGLS KLGI+GFG+GGG           ACFLIGASLYGTEVD
Sbjct: 162 AEDIGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVD 221

Query: 207 PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEED 266
           PSLATKVKVPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVHNPGSPEED
Sbjct: 222 PSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEED 281

Query: 267 DDAEQAFLMIRNWLHHGLVATNDLANCHG 285
           DDAEQAFLM+RNWLHHGLVATN+LANCHG
Sbjct: 282 DDAEQAFLMLRNWLHHGLVATNNLANCHG 310

BLAST of PI0026882 vs. NCBI nr
Match: XP_004141569.1 (uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical protein Csa_008898 [Cucumis sativus])

HSP 1 Score: 491.9 bits (1265), Expect = 3.7e-135
Identity = 236/269 (87.73%), Postives = 250/269 (92.94%), Query Frame = 0

Query: 27  KYNNRGVWKWKGKLRYSVKGRVK-DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 86
           KY+N GVW+WKGKLRYSV+GR+K DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN
Sbjct: 42  KYSNMGVWRWKGKLRYSVEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNN 101

Query: 87  NGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRI 146
           NGTAILLLSDLFAFQHSS+RDFAYRLAC GFNVLLPDLVHED WEKPPA+H AKQEMQRI
Sbjct: 102 NGTAILLLSDLFAFQHSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRI 161

Query: 147 AEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGG----------DACFLIGASLYGTEVD 206
           AEDIGTVTKWLT+EFSAAGLS KLGI+GFGYGGG            CFLIGAS+YGT+VD
Sbjct: 162 AEDIGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVD 221

Query: 207 PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEED 266
           PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVHNPGSPEED
Sbjct: 222 PSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEED 281

Query: 267 DDAEQAFLMIRNWLHHGLVATNDLANCHG 285
           DDAE+AFLM+RNWLHHGLVA N+LANCHG
Sbjct: 282 DDAEKAFLMLRNWLHHGLVAINNLANCHG 310

BLAST of PI0026882 vs. NCBI nr
Match: XP_038890567.1 (hydrolase tropI [Benincasa hispida])

HSP 1 Score: 437.6 bits (1124), Expect = 8.4e-119
Identity = 225/291 (77.32%), Postives = 235/291 (80.76%), Query Frame = 0

Query: 10  RVHISTPLMITSFEL-----YEKYNNRGVWKWK-------GKLRYSVKGRVKDDDDD-AC 69
           R  I TP  +  F       + K  NR  WKWK       GK+ YSV+G   DDDDD AC
Sbjct: 22  RRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKKGGKVCYSVEGVEDDDDDDAAC 81

Query: 70  ELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRDFAYRLACNGF 129
           ELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSS+RDFAYRLACNGF
Sbjct: 82  ELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLACNGF 141

Query: 130 NVLLPDLVHEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAGLSTKLGIMGFGY 189
           NVLLPDL+H D W+K     S K++MQRIAEDIGTVTKWL DEFSAAGLS KLGIMGFGY
Sbjct: 142 NVLLPDLIHGDSWKK----QSTKRDMQRIAEDIGTVTKWLVDEFSAAGLSRKLGIMGFGY 201

Query: 190 GGGD----------ACFLIGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLE 249
           GGG           ACF IGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLE
Sbjct: 202 GGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLE 261

Query: 250 KGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEQAFLMIRNWLHHGLVATN 278
           K MGEGSKLVIFGGRGH FVHNP SPEED+DAEQAFLMIRNWLHHGLVATN
Sbjct: 262 KRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMIRNWLHHGLVATN 308

BLAST of PI0026882 vs. NCBI nr
Match: XP_022155217.1 (hydrolase tropI [Momordica charantia])

HSP 1 Score: 394.8 bits (1013), Expect = 6.2e-106
Identity = 195/283 (68.90%), Postives = 225/283 (79.51%), Query Frame = 0

Query: 11  VHISTPLMITSFELYEKYNNRGVWKWKGKL-----RYSVKGRVKDDDDDACELVNGMELS 70
           +HI TP       L+ +  N GVWKWK K      +     R++ + D+ CELV+GMELS
Sbjct: 14  LHIGTPF---PSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEGCELVSGMELS 73

Query: 71  IGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVH 130
           IGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAFQ SS+ DFAYR+ACNGFNVLLPD+ H
Sbjct: 74  IGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFH 133

Query: 131 EDPW-EKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGGD---- 190
            DPW +K P + +AKQ+MQR+AEDIGT TKWL +EFSAAG+STKLGIMGFGYGGG     
Sbjct: 134 GDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDV 193

Query: 191 ------ACFLIGASLYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSK 250
                 ACF IGAS YGT +DPSL ++VKVPVLLISGDNDPLCPVSVMKDLEKG+G+GSK
Sbjct: 194 LARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGKGSK 253

Query: 251 LVIFGGRGHGFVHNPGSPEEDDDAEQAFLMIRNWLHHGLVATN 278
           LVIFGGRGHGF H PGSPEED+DAE+AFLM+RNWLH GLV TN
Sbjct: 254 LVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTN 293

BLAST of PI0026882 vs. NCBI nr
Match: XP_023536049.1 (carboxymethylenebutenolidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372.1 bits (954), Expect = 4.3e-99
Identity = 195/266 (73.31%), Postives = 212/266 (79.70%), Query Frame = 0

Query: 29  NNRGVWKWK-----GKLRYSVKGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKA 88
           NN G  K K     GKL YS +G VKD  D+++ACELVNGMELSIGE+ HHSI AYLFKA
Sbjct: 39  NNIGQLKGKREKKGGKLCYSAEG-VKDEIDEEEACELVNGMELSIGEEAHHSITAYLFKA 98

Query: 89  VKNNNGTAILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQE 148
           VKNNNGTAILLL+DLFAFQ SS+RDFAYRLACNGFNVLLP++ H D             E
Sbjct: 99  VKNNNGTAILLLTDLFAFQDSSIRDFAYRLACNGFNVLLPNIFHGD-----------SSE 158

Query: 149 MQRIAEDIGTVTKWLTDEFSAAGLSTKLGIMGFGYGGG----------DACFLIGASLYG 208
            Q+I EDIG  TKWL DEFSAAG+S KLGI+GFGYGGG           + F IGASLYG
Sbjct: 159 EQKIVEDIGAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLARDQGSYFSIGASLYG 218

Query: 209 TEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGS 268
           TEVDPSLATKV+VPVLLISGDND LCPVSVMKDLEKGMG+GSKLVIFGGRGHGFVH PGS
Sbjct: 219 TEVDPSLATKVEVPVLLISGDNDTLCPVSVMKDLEKGMGDGSKLVIFGGRGHGFVHKPGS 278

Query: 269 PEEDDDAEQAFLMIRNWLHHGLVATN 278
           PEED+DAEQAFLM+RNWLH GLVATN
Sbjct: 279 PEEDEDAEQAFLMLRNWLHDGLVATN 292

BLAST of PI0026882 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.2e-69
Identity = 125/240 (52.08%), Postives = 167/240 (69.58%), Query Frame = 0

Query: 50  DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRDFAY 109
           D++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDFAY
Sbjct: 76  DEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAY 135

Query: 110 RLACNGFNVLLPDLVHEDPWEK--PPAQHS---AKQEMQRIAEDIGTVTKWLTDEFSAAG 169
           R+ACNG+NVL+PDL   DPW K  P +++       +  RI +D  T TKW+ +EF+AAG
Sbjct: 136 RVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAG 195

Query: 170 LSTKLGIMGFGYGGG----------DACFLIGASLYGTEVDPSLATKVKVPVLLISGDND 229
           +S KLG+MGF +GGG             F  G S YGT +D ++A  VKVPVL I+GD D
Sbjct: 196 ISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRD 255

Query: 230 PLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEQAFLMIRNWLHHGLV 275
           PLC V  + ++E+ +GEGSK+V++ GRGHGFVH P +PE+D DAE+AF ++RNWLHH L+
Sbjct: 256 PLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 310

BLAST of PI0026882 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.2e-69
Identity = 125/240 (52.08%), Postives = 167/240 (69.58%), Query Frame = 0

Query: 50  DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSVRDFAY 109
           D++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDFAY
Sbjct: 81  DEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAY 140

Query: 110 RLACNGFNVLLPDLVHEDPWEK--PPAQHS---AKQEMQRIAEDIGTVTKWLTDEFSAAG 169
           R+ACNG+NVL+PDL   DPW K  P +++       +  RI +D  T TKW+ +EF+AAG
Sbjct: 141 RVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAG 200

Query: 170 LSTKLGIMGFGYGGG----------DACFLIGASLYGTEVDPSLATKVKVPVLLISGDND 229
           +S KLG+MGF +GGG             F  G S YGT +D ++A  VKVPVL I+GD D
Sbjct: 201 ISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRD 260

Query: 230 PLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEQAFLMIRNWLHHGLV 275
           PLC V  + ++E+ +GEGSK+V++ GRGHGFVH P +PE+D DAE+AF ++RNWLHH L+
Sbjct: 261 PLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 315

BLAST of PI0026882 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.7e-05
Identity = 44/190 (23.16%), Postives = 83/190 (43.68%), Query Frame = 0

Query: 90  ILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRIAEDI 149
           +LL+SD+F F+  ++R  A ++A +GF V++PD    DP+            ++    D 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 150 G-TVTKWLTDEFSAAGLSTKLGIMGFGYG----GGDACFLIGASLYGTEVDPSLATKVKV 209
           G   TK + +     G+ T +G  G  +G      +        L+ + V+       K 
Sbjct: 102 GFENTKPVLETIKNKGI-TAIGAAGMCWGVVELSKEELIQAAVLLHPSFVNVDDIKGGKA 161

Query: 210 PVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HNPGSPEEDDDA 269
           P+ ++  + D + P +++K  E+ +       S + I     HG+   +N   PE    A
Sbjct: 162 PIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAVKAA 221

BLAST of PI0026882 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 3.0e-05
Identity = 47/193 (24.35%), Postives = 82/193 (42.49%), Query Frame = 0

Query: 90  ILLLSDLFAFQHSSVRDFAYRLACNGFNVLLPDLVHEDPWEKPPAQHSAKQEMQRIAEDI 149
           +LL+SD+F F+  ++R  A ++A +GF V++PD    DP+            ++    D 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 150 G-TVTKWLTDEFSAAGLSTKLGIMGFGYGGGDACFLIGASLYGTEV--DPSLAT-----K 209
           G   TK + +     G+ T +G  G  +G      L    L    V   PS         
Sbjct: 102 GFENTKPVLETIKNKGI-TAIGAAGMCWGAKVVVELSKEELIQAAVLLHPSFVNVDDIKG 161

Query: 210 VKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HNPGSPEED 269
            K P+ ++  + D + P +++K  E+ +       S + I     HG+   +N   PE  
Sbjct: 162 GKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAV 221