PI0026749 (gene) Melon (PI 482460) v1

Overview
NamePI0026749
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCytochrome P450
Locationchr04: 27810408 .. 27813605 (+)
RNA-Seq ExpressionPI0026749
SyntenyPI0026749
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACAGCCCGCTATGATCACCGGAATAATCCTCTCCCTTCTTTTCTTTCTCTACACTGTTTTCGTTATGTCCCGGAGATCCGTCGCTCAACGGAAAACACTCCCACCAGAGGTTGATGGCGCTTGGCCGGTGATCGGCCACCTCCATCTTCTAAATGCAAGTGAGCCAACCCACATAACATTGGCAAAAATGGCGGATGCGTATGGACCAATATTCATGTTTAGATTCGGTAGAAAAAGAGTATTGATTGTGAGCAATTGGGAAATAGCAAAAGAGTGTTTTACTACAAACGATAAAATCTTTGCTTCGCGCCCAAAGTTGTTCGCATCAAAGCTTCTTGCCTATAACTATTGCATGATCGGATTCAGCGCATACAATCCACACTGGCGCTATGTCCGCAAAATAGCGACCATTGGACTTCTCAGGAACCACCACATGCCCCAACTCCAAAAAATAAGAGAATTAGAGGTTCGAAACTGGATGAAGGAACTCAACGACTTTTGTTTGCTTCAAAACAATAAAAGAAGTGAGGAAGTTTTTGTGGACATGAAAAAGTGGTTTGAAGACATAATCTTCAATATCATATTTAAAATGGTGATTGGAAAGCGATTTGCCACTGCTTTCGACGACCCTGTGTGCCGTGAAAAGTTCAGAAAGGCGTTGAATGATTTCCTTGAATTCTTTGGAAGATTCATTCCTTCAGATTCGTTTTCGATTTTGAGTTGGCTGGATTTGGGAGGACATGAGAAAGCCATGAAAAAGACTGCACAAACATTAGATGAGACGTTACACAAATTCCTCCAACAACATCGAGAGAGAAGAGATTGCAATAAAGGGAAAATTGAAGAACAAGACTTCATGGACGCAGTGATTTCTACGGTTGAAAATGATGACGGAGAAAACTTAAGCTACAATGCTGATACGATTATCAAAGCTACTTGCTTGGTAAAGTATATACTCTTATCTAGTTAGTATTGATTTTGAAATTGTTAAAATTATTTTTCTTGAGTTTAAAACAAGTAATTAGCGTTGTTTGGAAGACGTTTTAATTTTAGTAGTTTAATTTAATAAATAAGTAGTTTTAGAAAAAAATTGAGACTCAAGATAGTTTTTGAAATGTATTTGAAATAGTTTTTATGAATATAGTTTAAATAAATAATTTTTTTAAAAAAATATTTTCTTCTCAAATCAATCCAAATAAAGATATCTAATTCTCACAAAATTAATTAAATATTTAATTTTAGATTTCTAACACAATTTTCATATTATCAAAATTGATTTTGAATGATTAACTATTTTCGAATCATACTCAAACAAGTTTGGAATACTTTAACAATGATCATAATAGATTATAATATGTATGTAGGGTAATACATTTCCGTTGACATTTTCATCCATATATTTTTTCAAACGATAATTCACATTTCAATATATCTTAAAATGACATTTCATAAAAAAAATCAAGTAACGTTACATGAAAACAATAAAATAAAATAAATATAATATAGTTATGAAGCATTTTATTTTAATTTAAATGATAGAAACTACAAAAAAAAAAATATTTTAAAAATATAACAAAATATTGCAGTGTATTAAAGATATTGTTTAAAAAGCAAAATTTCGATATGTTGGTAAATATTTTAATTCATTTTGCTATATTTGAAAACAATCCCAAATTAATGGTCAAAATTCATAAATTTTAAGAAGAAAAAGTTATCTTTAGTGTAGTTTTATAAGAAAAATCCAATTTAGGGTTAAGATTACTAAGATTGAATAATATAATTTAATGAAATTGAAAGTTTGGTAAGTTTGGGGTGAAGGTTGAATTTCGTTTCCAGTAGGGAGATTTTGGTGATTAAAATGGAAAGAATTTAAAAAGTTTATAAGTTGCAACAAGTGTATGGAAGATTTTGTGATTAATTTTGATGACTATTTTGTTTTCCAAAATTAAGGTTATGTAACAGGTGTTCTAAAAAATATATAGTTAAATTTTGAATATTAAAAATGTTTCTTTAAATTTATTATTATTTTTTATATATCTATTAAATTTATCTAATCTTTTAAAGTCTAGACCTTCAAAAAAAATTAAAAAAAAAAAAAAAAATCATCTAAAATTTTAAATAGTTAACAAATGAGTATAAAATTTGAAAAAATTATTTCCTTGTTAAAATAGAATTTTTAGCGTGAAATATTAGCGATATTTATTATTATGATTATTAGGAGATTTTTTAAATATAGAAAATGAACCAAACTATTTGTAAGAGTAAAATTTTCTACATTTCTCACCGCCTAAATAGAGGTTGATAGAAGTTTTCATTAGTAGAAATCTATTATTTATAAAAGTCGTCTGAAATTATTTTTCCTATTATTAGATATAGAGTAAAGTTTAATAGTCTTAGAAGACCTAAAGCTTTGCAGAAATTCATTCTAAATTTTTGGTTGGTCTAAAAAAATTGGATATAAATAGAAATAATAAACTATTAGTTTAAAATAATGCAGAACATGATAGTAGGTGGAATTGACACTACAACCGTTACAATAACTTGGGCTCTTTCTTTACTTCTCAACAACGAAGAAATATTGAGGAAGGCTCAACTTGAAATAGACGAACAAATTGGGAGAGAAAGAGAAGTGAAAGAATCAGATATAAACAATCTATTATATGTCAACGCTATTGTGAAGGAAACTCTACGATTACATCCACCTGGACCACTTTTAGTCCCTCACGAGTCTATAGAAGATTGTGCAATTGTTGGCTACCACATTCCAAAAGGGACCCAGCTAATTGTTAATGCTCAAAAGCTCCAAAAAGACCCAATGGTTTGGGAGGATCCATGTGAATTTCGACCGGAAAGATTTTTGACAAGGGAAAATAATGTTGATCTTAGAGGAAAAAACCCAAAATTGATACCATTTGGTAATGGTCGAAGGATTTGCCCAGCCATCATTTGCTCTGCAAATGTTATATCTAACACTAGCAAACCTTTTACATGGGTTCAAAATTGAAAGGCCATCAGAAGCGCTTCTTGACATGGAAGAGAGTACTGGATTGGCTAGTGGCAGAAAAGCTCCGCTCGAAGTTATTTTAACTCCGTGCCTTCCCGCCAAAGTGTACGAGTAATTTTAAGATAAAGTTTGAGAGTAAATGTGTGAGAGTCTTTTGAAAGTGCATAATATATACATAATTCTATTAAA

mRNA sequence

ATGGAACAGCCCGCTATGATCACCGGAATAATCCTCTCCCTTCTTTTCTTTCTCTACACTGTTTTCGTTATGTCCCGGAGATCCGTCGCTCAACGGAAAACACTCCCACCAGAGGTTGATGGCGCTTGGCCGGTGATCGGCCACCTCCATCTTCTAAATGCAAGTGAGCCAACCCACATAACATTGGCAAAAATGGCGGATGCGTATGGACCAATATTCATGTTTAGATTCGGTAGAAAAAGAGTATTGATTGTGAGCAATTGGGAAATAGCAAAAGAGTGTTTTACTACAAACGATAAAATCTTTGCTTCGCGCCCAAAGTTGTTCGCATCAAAGCTTCTTGCCTATAACTATTGCATGATCGGATTCAGCGCATACAATCCACACTGGCGCTATGTCCGCAAAATAGCGACCATTGGACTTCTCAGGAACCACCACATGCCCCAACTCCAAAAAATAAGAGAATTAGAGGTTCGAAACTGGATGAAGGAACTCAACGACTTTTGTTTGCTTCAAAACAATAAAAGAAGTGAGGAAGTTTTTGTGGACATGAAAAAGTGGTTTGAAGACATAATCTTCAATATCATATTTAAAATGGTGATTGGAAAGCGATTTGCCACTGCTTTCGACGACCCTGTGTGCCGTGAAAAGTTCAGAAAGGCGTTGAATGATTTCCTTGAATTCTTTGGAAGATTCATTCCTTCAGATTCGTTTTCGATTTTGAGTTGGCTGGATTTGGGAGGACATGAGAAAGCCATGAAAAAGACTGCACAAACATTAGATGAGACGTTACACAAATTCCTCCAACAACATCGAGAGAGAAGAGATTGCAATAAAGGGAAAATTGAAGAACAAGACTTCATGGACGCAGTGATTTCTACGGTTGAAAATGATGACGGAGAAAACTTAAGCTACAATGCTGATACGATTATCAAAGCTACTTGCTTGAACATGATAGTAGGTGGAATTGACACTACAACCGTTACAATAACTTGGGCTCTTTCTTTACTTCTCAACAACGAAGAAATATTGAGGAAGGCTCAACTTGAAATAGACGAACAAATTGGGAGAGAAAGAGAAGTGAAAGAATCAGATATAAACAATCTATTATATGTCAACGCTATTGTGAAGGAAACTCTACGATTACATCCACCTGGACCACTTTTAGTCCCTCACGAGTCTATAGAAGATTGTGCAATTGTTGGCTACCACATTCCAAAAGGGACCCAGCTAATTGTTAATGCTCAAAAGCTCCAAAAAGACCCAATGGTTTGGGAGGATCCATGTGAATTTCGACCGGAAAGATTTTTGACAAGGGAAAATAATGTTGATCTTAGAGGAAAAAACCCAAAATTGATACCATTTGGTAATGGTCGAAGGATTTGCCCAGCCATCATTTGCTCTGCAAATGTTATATCTAACACTAGCAAACCTTTTACATGGGTTCAAAATTGAAAGGCCATCAGAAGCGCTTCTTGACATGGAAGAGAGTACTGGATTGGCTAGTGGCAGAAAAGCTCCGCTCGAAGTTATTTTAACTCCGTGCCTTCCCGCCAAAGTGTACGAGTAATTTTAAGATAAAGTTTGAGAGTAAATGTGTGAGAGTCTTTTGAAAGTGCATAATATATACATAATTCTATTAAA

Coding sequence (CDS)

ATGGAACAGCCCGCTATGATCACCGGAATAATCCTCTCCCTTCTTTTCTTTCTCTACACTGTTTTCGTTATGTCCCGGAGATCCGTCGCTCAACGGAAAACACTCCCACCAGAGGTTGATGGCGCTTGGCCGGTGATCGGCCACCTCCATCTTCTAAATGCAAGTGAGCCAACCCACATAACATTGGCAAAAATGGCGGATGCGTATGGACCAATATTCATGTTTAGATTCGGTAGAAAAAGAGTATTGATTGTGAGCAATTGGGAAATAGCAAAAGAGTGTTTTACTACAAACGATAAAATCTTTGCTTCGCGCCCAAAGTTGTTCGCATCAAAGCTTCTTGCCTATAACTATTGCATGATCGGATTCAGCGCATACAATCCACACTGGCGCTATGTCCGCAAAATAGCGACCATTGGACTTCTCAGGAACCACCACATGCCCCAACTCCAAAAAATAAGAGAATTAGAGGTTCGAAACTGGATGAAGGAACTCAACGACTTTTGTTTGCTTCAAAACAATAAAAGAAGTGAGGAAGTTTTTGTGGACATGAAAAAGTGGTTTGAAGACATAATCTTCAATATCATATTTAAAATGGTGATTGGAAAGCGATTTGCCACTGCTTTCGACGACCCTGTGTGCCGTGAAAAGTTCAGAAAGGCGTTGAATGATTTCCTTGAATTCTTTGGAAGATTCATTCCTTCAGATTCGTTTTCGATTTTGAGTTGGCTGGATTTGGGAGGACATGAGAAAGCCATGAAAAAGACTGCACAAACATTAGATGAGACGTTACACAAATTCCTCCAACAACATCGAGAGAGAAGAGATTGCAATAAAGGGAAAATTGAAGAACAAGACTTCATGGACGCAGTGATTTCTACGGTTGAAAATGATGACGGAGAAAACTTAAGCTACAATGCTGATACGATTATCAAAGCTACTTGCTTGAACATGATAGTAGGTGGAATTGACACTACAACCGTTACAATAACTTGGGCTCTTTCTTTACTTCTCAACAACGAAGAAATATTGAGGAAGGCTCAACTTGAAATAGACGAACAAATTGGGAGAGAAAGAGAAGTGAAAGAATCAGATATAAACAATCTATTATATGTCAACGCTATTGTGAAGGAAACTCTACGATTACATCCACCTGGACCACTTTTAGTCCCTCACGAGTCTATAGAAGATTGTGCAATTGTTGGCTACCACATTCCAAAAGGGACCCAGCTAATTGTTAATGCTCAAAAGCTCCAAAAAGACCCAATGGTTTGGGAGGATCCATGTGAATTTCGACCGGAAAGATTTTTGACAAGGGAAAATAATGTTGATCTTAGAGGAAAAAACCCAAAATTGATACCATTTGGTAATGGTCGAAGGATTTGCCCAGCCATCATTTGCTCTGCAAATGTTATATCTAACACTAGCAAACCTTTTACATGGGTTCAAAATTGA

Protein sequence

MEQPAMITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNTSKPFTWVQN
Homology
BLAST of PI0026749 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 3.4e-115
Identity = 209/467 (44.75%), Postives = 298/467 (63.81%), Query Frame = 0

Query: 11  ILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLAKMADAYG 70
           I S+L  ++    + R++   R    P V GAWP++GHL LLN S+  H  L  +AD YG
Sbjct: 15  IASILSLIFLCLFLYRKN--SRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALADKYG 74

Query: 71  PIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNPHW 130
           P+F  + G K  L++SNWE++KE FTTND   +SRPKL A ++++YN   +G + Y P+W
Sbjct: 75  PLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYW 134

Query: 131 RYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWFED 190
           R +RKI T   L N  + Q   IR  EVR  +KEL D     N   S    VD+K+W   
Sbjct: 135 RELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAY 194

Query: 191 IIFNIIFKMVIGKRF--ATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSWLDLGG 250
           + FN++ +MV+GKR+      +     ++F K + +F+   G F  +D    L WLDLGG
Sbjct: 195 LTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGG 254

Query: 251 HEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENLSYNAD 310
           HEKAMK  A+ +D+ L ++L++HR+++   +    ++DFMD +IS +  +  +  +++AD
Sbjct: 255 HEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISAL--NGAQIGAFDAD 314

Query: 311 TIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKESDINN 370
           TI KAT L +I+GG D+T VT+TWALSLLL N   L KA+ EID QIG++  ++ESDI+ 
Sbjct: 315 TICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISK 374

Query: 371 LLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPMVWEDP 430
           L+Y+ AIVKETLRL+PP P   P E  E+C + GYHI KGT+LI N  K+ +DP VW DP
Sbjct: 375 LVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDP 434

Query: 431 CEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
            EF+PERFLT   +VDLRG N +L+PFG+GRR+C  +    N++  T
Sbjct: 435 LEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFT 477

BLAST of PI0026749 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.1e-113
Identity = 210/470 (44.68%), Postives = 306/470 (65.11%), Query Frame = 0

Query: 10  IILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEP-THITLAKMADA 69
           + + +L F++    + ++S   +    P   GAWP+IGHLHLL   E   + TL KMAD 
Sbjct: 8   LFVPILVFVF--IALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADH 67

Query: 70  YGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNP 129
           YGP    + G     +VS++E+AK+CFT NDK  ASRP   A+K + YN+ + GF+ Y+ 
Sbjct: 68  YGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSA 127

Query: 130 HWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWF 189
            WR +RKIATI LL N  +  L+ +R  E+   +K+L  + L   N  ++ V VD+K W 
Sbjct: 128 FWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDL--YSLWFKNGGTKPVMVDLKSWL 187

Query: 190 EDIIFNIIFKMVIGKRF-----ATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSW 249
           ED+  N+I +MV GKR+     + + +D     + +KA+  F    G F  SD+F  LS+
Sbjct: 188 EDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSF 247

Query: 250 LDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENL 309
            DL GHEK MK+T   LD  L ++++ HR++R  +  K  + DF+D ++S  E     +L
Sbjct: 248 FDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHL 307

Query: 310 SYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKE 369
            Y+A+T IK+TCL +I+GG DT+  T+TWA+SLLLNN+E+L+KAQ EID  +GR+R V++
Sbjct: 308 QYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVED 367

Query: 370 SDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPM 429
           SDI NL+Y+ AI+KETLRL+P GPLL P E++EDC + GY++P GT+LIVN  K+Q+DP 
Sbjct: 368 SDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPK 427

Query: 430 VWEDPCEFRPERFLTRE-NNVDLRGKNPKLIPFGNGRRICPAIICSANVI 473
           V+ +P EFRPERF+T E    D+RG+N +L+PFG+GRR CP    +  V+
Sbjct: 428 VYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVL 473

BLAST of PI0026749 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.0e-111
Identity = 212/474 (44.73%), Postives = 290/474 (61.18%), Query Frame = 0

Query: 6   MITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLAKM 65
           ++ G  + L  F Y V+      + +    PP+  GAWP+IGHLHLL   +  H  L+  
Sbjct: 10  LLLGFFVLLPCFFYFVWKKPNNKIKE----PPQPAGAWPIIGHLHLLARGDLPHKILSSF 69

Query: 66  ADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSA 125
           AD  GP+F  + G  + L+V+N EIAKECFTTND+ F +RP   A+K++ YNY M+G + 
Sbjct: 70  ADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAP 129

Query: 126 YNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMK 185
           Y P+WR +RKI  +  L N  +  L+ +   E+    KEL      QN    +   VDMK
Sbjct: 130 YGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQN---IDFCLVDMK 189

Query: 186 KWFEDIIFNIIFKMVIGKRFATAFDDPVCRE----KFRKALNDFLEFFGRFIPSDSFSIL 245
           +W  D+  N+  KMV+GKRF  +     C E       K L +     G F+ SD    L
Sbjct: 190 QWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYL 249

Query: 246 SWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGE 305
            WLDLGGHEK MK+T + LD     +L +H+ +R     + ++QDFMD ++S +E     
Sbjct: 250 RWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEE---S 309

Query: 306 NLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREV 365
            L  + DTI K  CL +I+GG DTT  T+TWALSLLLNN   L+KAQ E+D  +GR+R V
Sbjct: 310 KLGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNV 369

Query: 366 KESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKD 425
            ESD+  L Y++AI+KETLRL+PPGPLL P    EDC I GYH+  GT+LIVNA K+Q+D
Sbjct: 370 DESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRD 429

Query: 426 PMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           P+VW  P E++PERFL  E +VD++G++ +LIPFG+GRR CPAI  +  V+  T
Sbjct: 430 PLVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLT 471

BLAST of PI0026749 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 399.4 bits (1025), Expect = 5.6e-110
Identity = 207/484 (42.77%), Postives = 300/484 (61.98%), Query Frame = 0

Query: 11  ILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLAKMADAYG 70
           ++SLLFFL+      R S       PP + G+WP++GHL L+  ++  H TL  + D YG
Sbjct: 16  LISLLFFLF------RFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGALVDKYG 75

Query: 71  PIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNPHW 130
           PIF  + G    L++SNWE+AKECFT ND + +SRPK  A +L++YN   IG++ Y  +W
Sbjct: 76  PIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYW 135

Query: 131 RYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKEL---------NDFCLLQNNKRS---- 190
           R +RKI T+ +L N  +  L  IR  EV+  +KEL         + + LL + K S    
Sbjct: 136 RQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSSTND 195

Query: 191 -----EEVFVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCRE--KFRKALNDFLEFFG 250
                + V V++KKWF  +  N++ +MV+GKR     D     E  +F + + DF+   G
Sbjct: 196 EPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFMRLIG 255

Query: 251 RFIPSDSFSILSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGK--IEEQDFM 310
            F   D    L WLDLGGHEK MKK A+  D  L+++L++HRE++        + E+DFM
Sbjct: 256 TFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKVVGERDFM 315

Query: 311 DAVISTVENDDGENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQ 370
           DA++  +++   E   ++ DTIIKAT L +I+GG DTT  T+TWA+ LLL +  +L K +
Sbjct: 316 DAMLLVLKDKPIE--GFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPHVLEKLK 375

Query: 371 LEIDEQIGREREVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKG 430
            E++  IG+ER V ESDIN L+Y++AI+KETLRL+PP P   P E  EDC I GYHI KG
Sbjct: 376 EELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKG 435

Query: 431 TQLIVNAQKLQKDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICS 473
           T+L+ N  K+ +DP VW DP EF+PERFL+   +VD+RG+N +L+PFG+GRR+C  +   
Sbjct: 436 TRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCAGMSLG 491

BLAST of PI0026749 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 397.5 bits (1020), Expect = 2.1e-109
Identity = 202/469 (43.07%), Postives = 302/469 (64.39%), Query Frame = 0

Query: 10  IILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEP-THITLAKMADA 69
           + + +L F++    + ++S   +    P   GAWP+IGHLHLL+  E   + TL KMAD 
Sbjct: 8   LFVPILVFVF--IALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQ 67

Query: 70  YGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNP 129
           YGP    R G     +VS++E+AK+CFT NDK  ASRP   A+K + Y+  + GF+ Y+ 
Sbjct: 68  YGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSA 127

Query: 130 HWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWF 189
            WR +RKIAT+ LL N  +  L+ +R  E+   M++L  + L      SE V VD+K W 
Sbjct: 128 FWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDL--YSLWVKKGGSEPVMVDLKSWL 187

Query: 190 EDIIFNIIFKMVIGKRF----ATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSWL 249
           ED+  N++ +MV GKR+    + + +D     + RK + +F    G F  SD+F  L W 
Sbjct: 188 EDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWF 247

Query: 250 DLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENLS 309
           D  GHEK MK+T + LD  L ++++ HR++R  +  K  + DF+D ++S  E     +L 
Sbjct: 248 DFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQ 307

Query: 310 YNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKES 369
           ++A T IK+TCL +I+GG +T+  T+TWA+SLLLNN+++L+KAQ EID  +GR+R V++S
Sbjct: 308 HDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDS 367

Query: 370 DINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPMV 429
           DI NL+Y+ AI+KETLRL+P GPLL   E+IEDC + GY++ +GT+++VN  K+Q+DP V
Sbjct: 368 DIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRV 427

Query: 430 WEDPCEFRPERFLTRE-NNVDLRGKNPKLIPFGNGRRICPAIICSANVI 473
           + +P EFRPERF+T E    D+RG+N +L+PFG+GRR CP    +  V+
Sbjct: 428 YMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVL 472

BLAST of PI0026749 vs. ExPASy TrEMBL
Match: A0A0A0LDQ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852620 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.8e-199
Identity = 343/478 (71.76%), Postives = 398/478 (83.26%), Query Frame = 0

Query: 1   MEQPA-MITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTH 60
           MEQP+  + G+I +L F +Y +F+MSRRSVA  K LPPE DGAWPVIGHLHLLNASEP H
Sbjct: 1   MEQPSHAVAGVIFALAFIIYLLFIMSRRSVAHPKRLPPEPDGAWPVIGHLHLLNASEPIH 60

Query: 61  ITLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYC 120
           ITLAKMAD YGPIF FRFG KR LIVSNWEIAKECFTTND+IFASRPK  ASKLLAY+Y 
Sbjct: 61  ITLAKMADVYGPIFTFRFGTKRALIVSNWEIAKECFTTNDRIFASRPKQLASKLLAYDYA 120

Query: 121 MIGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELND-FCLLQNNKRSE 180
           M+ FS YNPHWRYVRK+A +GL  N  + QL+ +R +EV +WMKEL D + L  NN +SE
Sbjct: 121 MMAFSPYNPHWRYVRKLAMLGLFTNQRIEQLEHVRVVEVHSWMKELYDLYWLKNNNNKSE 180

Query: 181 EVFVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSF 240
           +V V+MKKWF DI  N +FKMVIGKRF+TAFDD V REK RKAL  F EFF +FIPS+SF
Sbjct: 181 KVVVEMKKWFVDITLNTMFKMVIGKRFSTAFDDHVSREKCRKALGGFFEFFMKFIPSESF 240

Query: 241 SILSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRD-CNKGKIEEQDFMDAVISTVEN 300
             LSWLDLGGHEKAMKK+AQ LDE  HKFLQQHRERRD  + G++EE+DFMD +IS+VE+
Sbjct: 241 LFLSWLDLGGHEKAMKKSAQILDEVFHKFLQQHRERRDYYSNGQMEEKDFMDVMISSVED 300

Query: 301 DDGENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGR 360
           DDGE L+Y+ADTIIKATCLN+I+GG DTT VT++WALSLLLNNE  L+KAQ E+DEQ+GR
Sbjct: 301 DDGEQLNYDADTIIKATCLNVILGGFDTTAVTMSWALSLLLNNENALKKAQHELDEQVGR 360

Query: 361 EREVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQK 420
           ER+VKE+D+ NL Y+ AIVKETLRLHPPGPLLVP ESIEDC I  YHIPKGT+LIVNAQK
Sbjct: 361 ERQVKETDLKNLPYLQAIVKETLRLHPPGPLLVPRESIEDCTIGSYHIPKGTRLIVNAQK 420

Query: 421 LQKDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           LQKDP VW+DPCEFRPERF+T + N D+RG+NP+LIPFGNGRRICPAI  +  +I  T
Sbjct: 421 LQKDPHVWDDPCEFRPERFITNQKNFDVRGQNPQLIPFGNGRRICPAISFALQMIHLT 478

BLAST of PI0026749 vs. ExPASy TrEMBL
Match: A0A1S3CNU6 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502576 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 3.3e-198
Identity = 334/474 (70.46%), Postives = 398/474 (83.97%), Query Frame = 0

Query: 4   PAMITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLA 63
           P  + G+I +L+F +Y +F++SRRS    K  PPE DGAWPVIGHLHLLNASEP HITLA
Sbjct: 5   PHAVAGVIFALVFIIYLLFIVSRRSAGHPKRFPPEADGAWPVIGHLHLLNASEPIHITLA 64

Query: 64  KMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGF 123
           KMADAYGPIF FRFG KR LIVSNWEIAKECFTTND+IFASRPK  ASKLLAY+Y M+ F
Sbjct: 65  KMADAYGPIFTFRFGMKRALIVSNWEIAKECFTTNDRIFASRPKQLASKLLAYDYAMMVF 124

Query: 124 SAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELND-FCLLQNNKRSEEVFV 183
           S YN HWRYVRK+A +GL  N  + QL+ +R +EV +WMKEL + + L  NN +SE+V V
Sbjct: 125 SPYNQHWRYVRKLAMLGLFTNQRIEQLEHVRAVEVHSWMKELYELYWLKNNNNKSEKVVV 184

Query: 184 DMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILS 243
           +MKKWF DI  N +FKMV+GKRFATAFDD V REK RKAL  F EFF +FIPS+SFS  S
Sbjct: 185 EMKKWFVDITINTMFKMVVGKRFATAFDDHVSREKCRKALGGFFEFFIKFIPSESFSFFS 244

Query: 244 WLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRD-CNKGKIEEQDFMDAVISTVENDDGE 303
           WLDLGGHEKAMKKTAQ LDET HKFLQ+HRERRD C+ G++EE+DFMD +IS+VE++DGE
Sbjct: 245 WLDLGGHEKAMKKTAQILDETFHKFLQEHRERRDYCSTGEMEEKDFMDVMISSVEDEDGE 304

Query: 304 NLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREV 363
            L+Y+ADTIIKATCLN+I+GGIDT T+T++WALSLLLNNE  L+KAQLE++EQ+GR R+V
Sbjct: 305 QLNYDADTIIKATCLNVILGGIDTATITMSWALSLLLNNENALKKAQLELNEQVGRARQV 364

Query: 364 KESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKD 423
           KE+D+ NL Y+ AIVKETLRLHPPGPLLVPHESIEDC I  +HIPKGT+LIVNAQKLQKD
Sbjct: 365 KETDLKNLPYLQAIVKETLRLHPPGPLLVPHESIEDCTIGSHHIPKGTRLIVNAQKLQKD 424

Query: 424 PMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           P+VW++PCEFRPERF+T +NN D+RG+NP+LIPFGNGRR+CPAI  +  +I  T
Sbjct: 425 PLVWDNPCEFRPERFITNQNNFDVRGQNPQLIPFGNGRRMCPAISFALQMIHLT 478

BLAST of PI0026749 vs. ExPASy TrEMBL
Match: A0A6J1F5K6 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 8.0e-176
Identity = 310/479 (64.72%), Postives = 367/479 (76.62%), Query Frame = 0

Query: 1   MEQPAMITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHI 60
           ME    + GII   LFFLY +F  SRRSVA RK +PPE  GAWP+IGHLHLL+A +PTHI
Sbjct: 1   MEHLYALAGIIFPFLFFLYVLFTSSRRSVAHRKRIPPEAGGAWPLIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCM 120
            LAKMADAYGP+F  RFG K+ L+VS+WEIAKE  TTND+IFASRPKL ASKLLAY+Y M
Sbjct: 61  VLAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 IGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEV 180
           +GFS Y PHWR VRKIAT+ LL NH + QLQ IR  EV+ WMKEL+    +++N+R E+V
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMKELHKMS-IKSNERGEKV 180

Query: 181 FVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSI 240
            V+MKKW  DI  N IFKMVIGKRF+         E F KAL DF   F  F+PSDSF  
Sbjct: 181 AVEMKKWLADITLNTIFKMVIGKRFSAIQHG---TENFPKALIDFFGLFHIFVPSDSFPF 240

Query: 241 LSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDG 300
           LSWLDLGGHEK MKKTA+ +DE  HKFLQQHRERR+  + +  E+DFMD +I+ VE +DG
Sbjct: 241 LSWLDLGGHEKTMKKTAKIIDEAFHKFLQQHRERRNDGEAETAEKDFMDVMIARVE-EDG 300

Query: 301 ENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGRERE 360
           ++ SY+ADTIIKATCLNMIVGG DTT VT+TWAL LLLNNEE L+K QLE+DE++GR R+
Sbjct: 301 QHFSYDADTIIKATCLNMIVGGFDTTAVTMTWALCLLLNNEETLKKVQLELDEKVGRSRQ 360

Query: 361 VKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQK 420
           VKESD+ NL Y+ AIVKETLRL+P  PL+ PHES+EDC + GYHI KGT+LIVN QKLQK
Sbjct: 361 VKESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQK 420

Query: 421 DPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNTSKPF 480
           DP VWE+P EFRPERFLT + N D+RG +P+ IPFGNGRR CPAI  +  V+  T   F
Sbjct: 421 DPRVWEEPSEFRPERFLTGQKNFDVRGLSPQFIPFGNGRRRCPAISFALQVMHITLANF 474

BLAST of PI0026749 vs. ExPASy TrEMBL
Match: A0A6J1CCZ4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.4e-175
Identity = 299/469 (63.75%), Postives = 372/469 (79.32%), Query Frame = 0

Query: 11  ILSLLFFLYTVFVMSRRSVAQRKTL--PPEVDGAWPVIGHLHLLNASEPTHITLAKMADA 70
           + +LL FLY +F MSR+S A R+ L  PPE  GAWPVIGHLH L+A EPTHITLAKMADA
Sbjct: 14  VFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITLAKMADA 73

Query: 71  YGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNP 130
           YGP+F  RFG K+ L+VS+WEIA+E FTTND++FASRPKL ASKLL Y+Y M G S Y P
Sbjct: 74  YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFGLSPYGP 133

Query: 131 HWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWF 190
           HWR  R++AT+ LL NH + +LQ +R LEV+NW+KEL   C+    +  E+  V+MK WF
Sbjct: 134 HWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCV----ENEEKAVVEMKTWF 193

Query: 191 EDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSWLDLGG 250
            D+  N IF+MV+GKRF+TAFDD    E+ +KAL DF   FG FIPSD+F  LSW DLGG
Sbjct: 194 GDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFLSWFDLGG 253

Query: 251 HEKAMKKTAQTLDETLHKFLQQHRERRDCNKG--KIEEQDFMDAVISTVENDDGENLSYN 310
           HEKAMK+TA+ LD+T  KFL++H++++  N G  ++EEQDFMD +IS V+N + E+ SY+
Sbjct: 254 HEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN-EAESFSYD 313

Query: 311 ADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKESDI 370
           A+T+IKATCLNMI+GG DTTTVT+TWA+SLLLNN+E L+KAQLE+DEQ+GRER+V ESD+
Sbjct: 314 AETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNESDV 373

Query: 371 NNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPMVWE 430
            NLLY+ AIVKETLRL+PP PLL PHESIEDC  VGY+IP GT+LIVN +KLQ+DP VWE
Sbjct: 374 KNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFVWE 433

Query: 431 DPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           DPCEFRPERFLT +NN D+RG++P+ IPFGNGRR+CP I  +  VI  T
Sbjct: 434 DPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLT 477

BLAST of PI0026749 vs. ExPASy TrEMBL
Match: A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.3e-173
Identity = 306/479 (63.88%), Postives = 365/479 (76.20%), Query Frame = 0

Query: 1   MEQPAMITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHI 60
           ME    + GII   LFF+Y +F  SRRSVA RK +PPE  GAWPVIGHLHLL+A +PTHI
Sbjct: 1   MEHLYALAGIIFPFLFFVYVLFTSSRRSVAHRKRIPPEAGGAWPVIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCM 120
            LAKMADAYGP+F  RFG K+ L+VS+WEIAKE  TTND+IFASRPKL ASKLLAY+Y M
Sbjct: 61  ALAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 IGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEV 180
           +GFS Y PHWR VRKIAT+ LL NH + QLQ IR  EV+ WMKEL+    +++N+R ++V
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMS-IRSNERGDKV 180

Query: 181 FVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSI 240
            V+MKKW  DI  N IFKMVIGKRF+         E F KAL DF   F  F+PSDSF  
Sbjct: 181 AVEMKKWLADITLNTIFKMVIGKRFSAIQHG---TENFPKALIDFFGLFHIFVPSDSFPF 240

Query: 241 LSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDG 300
           LSWLDLGGHEK MKKTA+ +DE  HKFLQQHRERR+  + +  EQDFMD +IS VE D+ 
Sbjct: 241 LSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNEGQAETAEQDFMDVMISRVEEDE- 300

Query: 301 ENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGRERE 360
           ++ SY+ADT+IKATCLNMIVGG DTT VT+TWAL LLLNNEE L+K QLE+DE++GR  +
Sbjct: 301 QHFSYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALKKVQLELDEKVGRSTQ 360

Query: 361 VKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQK 420
           VKESD+ NL Y+ AIVKETLRL+P  PL+ PHES+EDC + GYHI KGT+LIVN QKLQK
Sbjct: 361 VKESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQK 420

Query: 421 DPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNTSKPF 480
           DP VWE+P EF PERF+T + N D+RG +P+ IPFGNGRR+CPAI  +  V+  T   F
Sbjct: 421 DPRVWEEPNEFCPERFVTSQKNFDVRGLSPQFIPFGNGRRMCPAISFALQVMHITLANF 474

BLAST of PI0026749 vs. NCBI nr
Match: XP_004147804.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59913.1 hypothetical protein Csa_000874 [Cucumis sativus])

HSP 1 Score: 705.3 bits (1819), Expect = 3.7e-199
Identity = 343/478 (71.76%), Postives = 398/478 (83.26%), Query Frame = 0

Query: 1   MEQPA-MITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTH 60
           MEQP+  + G+I +L F +Y +F+MSRRSVA  K LPPE DGAWPVIGHLHLLNASEP H
Sbjct: 1   MEQPSHAVAGVIFALAFIIYLLFIMSRRSVAHPKRLPPEPDGAWPVIGHLHLLNASEPIH 60

Query: 61  ITLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYC 120
           ITLAKMAD YGPIF FRFG KR LIVSNWEIAKECFTTND+IFASRPK  ASKLLAY+Y 
Sbjct: 61  ITLAKMADVYGPIFTFRFGTKRALIVSNWEIAKECFTTNDRIFASRPKQLASKLLAYDYA 120

Query: 121 MIGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELND-FCLLQNNKRSE 180
           M+ FS YNPHWRYVRK+A +GL  N  + QL+ +R +EV +WMKEL D + L  NN +SE
Sbjct: 121 MMAFSPYNPHWRYVRKLAMLGLFTNQRIEQLEHVRVVEVHSWMKELYDLYWLKNNNNKSE 180

Query: 181 EVFVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSF 240
           +V V+MKKWF DI  N +FKMVIGKRF+TAFDD V REK RKAL  F EFF +FIPS+SF
Sbjct: 181 KVVVEMKKWFVDITLNTMFKMVIGKRFSTAFDDHVSREKCRKALGGFFEFFMKFIPSESF 240

Query: 241 SILSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRD-CNKGKIEEQDFMDAVISTVEN 300
             LSWLDLGGHEKAMKK+AQ LDE  HKFLQQHRERRD  + G++EE+DFMD +IS+VE+
Sbjct: 241 LFLSWLDLGGHEKAMKKSAQILDEVFHKFLQQHRERRDYYSNGQMEEKDFMDVMISSVED 300

Query: 301 DDGENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGR 360
           DDGE L+Y+ADTIIKATCLN+I+GG DTT VT++WALSLLLNNE  L+KAQ E+DEQ+GR
Sbjct: 301 DDGEQLNYDADTIIKATCLNVILGGFDTTAVTMSWALSLLLNNENALKKAQHELDEQVGR 360

Query: 361 EREVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQK 420
           ER+VKE+D+ NL Y+ AIVKETLRLHPPGPLLVP ESIEDC I  YHIPKGT+LIVNAQK
Sbjct: 361 ERQVKETDLKNLPYLQAIVKETLRLHPPGPLLVPRESIEDCTIGSYHIPKGTRLIVNAQK 420

Query: 421 LQKDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           LQKDP VW+DPCEFRPERF+T + N D+RG+NP+LIPFGNGRRICPAI  +  +I  T
Sbjct: 421 LQKDPHVWDDPCEFRPERFITNQKNFDVRGQNPQLIPFGNGRRICPAISFALQMIHLT 478

BLAST of PI0026749 vs. NCBI nr
Match: XP_008464768.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 701.0 bits (1808), Expect = 6.9e-198
Identity = 334/474 (70.46%), Postives = 398/474 (83.97%), Query Frame = 0

Query: 4   PAMITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLA 63
           P  + G+I +L+F +Y +F++SRRS    K  PPE DGAWPVIGHLHLLNASEP HITLA
Sbjct: 5   PHAVAGVIFALVFIIYLLFIVSRRSAGHPKRFPPEADGAWPVIGHLHLLNASEPIHITLA 64

Query: 64  KMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGF 123
           KMADAYGPIF FRFG KR LIVSNWEIAKECFTTND+IFASRPK  ASKLLAY+Y M+ F
Sbjct: 65  KMADAYGPIFTFRFGMKRALIVSNWEIAKECFTTNDRIFASRPKQLASKLLAYDYAMMVF 124

Query: 124 SAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELND-FCLLQNNKRSEEVFV 183
           S YN HWRYVRK+A +GL  N  + QL+ +R +EV +WMKEL + + L  NN +SE+V V
Sbjct: 125 SPYNQHWRYVRKLAMLGLFTNQRIEQLEHVRAVEVHSWMKELYELYWLKNNNNKSEKVVV 184

Query: 184 DMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILS 243
           +MKKWF DI  N +FKMV+GKRFATAFDD V REK RKAL  F EFF +FIPS+SFS  S
Sbjct: 185 EMKKWFVDITINTMFKMVVGKRFATAFDDHVSREKCRKALGGFFEFFIKFIPSESFSFFS 244

Query: 244 WLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRD-CNKGKIEEQDFMDAVISTVENDDGE 303
           WLDLGGHEKAMKKTAQ LDET HKFLQ+HRERRD C+ G++EE+DFMD +IS+VE++DGE
Sbjct: 245 WLDLGGHEKAMKKTAQILDETFHKFLQEHRERRDYCSTGEMEEKDFMDVMISSVEDEDGE 304

Query: 304 NLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREV 363
            L+Y+ADTIIKATCLN+I+GGIDT T+T++WALSLLLNNE  L+KAQLE++EQ+GR R+V
Sbjct: 305 QLNYDADTIIKATCLNVILGGIDTATITMSWALSLLLNNENALKKAQLELNEQVGRARQV 364

Query: 364 KESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKD 423
           KE+D+ NL Y+ AIVKETLRLHPPGPLLVPHESIEDC I  +HIPKGT+LIVNAQKLQKD
Sbjct: 365 KETDLKNLPYLQAIVKETLRLHPPGPLLVPHESIEDCTIGSHHIPKGTRLIVNAQKLQKD 424

Query: 424 PMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           P+VW++PCEFRPERF+T +NN D+RG+NP+LIPFGNGRR+CPAI  +  +I  T
Sbjct: 425 PLVWDNPCEFRPERFITNQNNFDVRGQNPQLIPFGNGRRMCPAISFALQMIHLT 478

BLAST of PI0026749 vs. NCBI nr
Match: XP_038903603.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 700.7 bits (1807), Expect = 9.0e-198
Identity = 329/476 (69.12%), Postives = 400/476 (84.03%), Query Frame = 0

Query: 1   MEQPAMITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHI 60
           MEQP  +TGI  +L+FF + +F++SRRSV QRK LPPE DGAWPVIGHLHLLN  EPTH 
Sbjct: 1   MEQPHAVTGIFFALVFFFFAIFIISRRSVGQRKRLPPEADGAWPVIGHLHLLNTEEPTHK 60

Query: 61  TLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCM 120
           TL+KMADAYGPIF FRFGRK+ LIVSNW+IA ECFTTND+IFASRPKL ASKLLAY+Y M
Sbjct: 61  TLSKMADAYGPIFTFRFGRKKALIVSNWKIAMECFTTNDRIFASRPKLLASKLLAYDYAM 120

Query: 121 IGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEV 180
           + FS YNP+WR+VRK+AT+GL  N    QL++IR  EV  WMKEL++  +L+N+K+SE+V
Sbjct: 121 MAFSPYNPYWRHVRKLATLGLFTNRRTEQLERIRSFEVHRWMKELHELYVLENSKKSEKV 180

Query: 181 FVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSI 240
            V+MK+WF DI  + + KMVIGKRFATAF+D   REK+RKAL  F EFFG+FIPS+S+  
Sbjct: 181 VVEMKRWFVDITLDTMLKMVIGKRFATAFNDLGGREKWRKALEGFFEFFGKFIPSESYPF 240

Query: 241 LSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRD-CNKGKIEEQDFMDAVISTVENDD 300
           LSWLD+GGHE+AMKK AQ LDE  H FLQQHRERRD CNKG +EEQDFMD +ISTVE+DD
Sbjct: 241 LSWLDIGGHEEAMKKNAQILDEAFHIFLQQHRERRDYCNKGDMEEQDFMDVMISTVEDDD 300

Query: 301 GENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGRER 360
           G++  Y+ADTIIKATCLN+I+GG DTT++T+ WALSLLLNNE+ L+KAQLE+DEQIGRER
Sbjct: 301 GQHFDYDADTIIKATCLNVILGGFDTTSITMIWALSLLLNNEDALKKAQLELDEQIGRER 360

Query: 361 EVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQ 420
           +VKE+D+ NL+Y+ AIVKETLRL+P GPLL+PHESIEDC I GYHI +GT+LIVNAQKLQ
Sbjct: 361 QVKETDVKNLIYLQAIVKETLRLYPAGPLLIPHESIEDCTISGYHISRGTRLIVNAQKLQ 420

Query: 421 KDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           KDP++WE   EFRPERFLT +  +D+RG++P+LIPFGNGRR+CPAI  +  +I  T
Sbjct: 421 KDPLIWEHASEFRPERFLTSQKYIDVRGQSPQLIPFGNGRRMCPAISSALQIIHIT 476

BLAST of PI0026749 vs. NCBI nr
Match: XP_038899739.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 700.7 bits (1807), Expect = 9.0e-198
Identity = 335/476 (70.38%), Postives = 395/476 (82.98%), Query Frame = 0

Query: 1   MEQPAMITGIILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHI 60
           MEQ   +TGII +L+FF Y +F+M RRS  +RK LPPE  G WPVIGHLHLL+  EP HI
Sbjct: 1   MEQTHAVTGIIFALVFFFYAMFIMHRRSFDKRKRLPPEAGGTWPVIGHLHLLSTKEPIHI 60

Query: 61  TLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCM 120
           TLAKMADAYGPIF FRFGRK+ LIVSNWEIA ECF TND+IFASRPKL ASKLLAY+Y M
Sbjct: 61  TLAKMADAYGPIFTFRFGRKKALIVSNWEIAAECFITNDRIFASRPKLLASKLLAYDYAM 120

Query: 121 IGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEV 180
           +GFS YNP+WRY+RK+AT+GL  N  + QL+++R  EV  WMKEL +    QNNK+SE+V
Sbjct: 121 VGFSPYNPYWRYIRKLATLGLFTNKRVKQLERVRASEVHCWMKELYELYCHQNNKKSEKV 180

Query: 181 FVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSI 240
            V+MKK F D+  + IFKMVIGKRFATAFDD V  EK RKAL DFL+FFG+FIPS+SF  
Sbjct: 181 VVEMKKRFVDVTLDTIFKMVIGKRFATAFDDHVGSEKCRKALKDFLKFFGKFIPSESFPF 240

Query: 241 LSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERR-DCNKGKIEEQDFMDAVISTVENDD 300
           LSWLD+G HEKAM KTAQ LDETLHKFLQQHRER+  CNKG++EEQDFMD +IS VE D 
Sbjct: 241 LSWLDIGRHEKAMNKTAQILDETLHKFLQQHRERKYYCNKGEMEEQDFMDVMISAVEEDH 300

Query: 301 GENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGRER 360
           G + SY+ADTIIKATCLN+I+GG DT +VT+TWALSLLLNNE+ L+KAQLE+D+QIGRER
Sbjct: 301 GGHFSYDADTIIKATCLNLILGGFDTMSVTMTWALSLLLNNEDALKKAQLELDKQIGRER 360

Query: 361 EVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQ 420
           +VKESD+ NLLY+ AIVKETLRLHPPGPLLVP ES EDC I GYHIP+G +L++NAQKLQ
Sbjct: 361 QVKESDVKNLLYIQAIVKETLRLHPPGPLLVPRESTEDCTISGYHIPRGIRLVINAQKLQ 420

Query: 421 KDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVISNT 476
           KDP++WE  CEFRPERFLT +  +D+RG++P+LIPFGNGRRICPAI  S N++  T
Sbjct: 421 KDPLIWEHACEFRPERFLTSQKYIDVRGQSPQLIPFGNGRRICPAISFSLNIMHLT 476

BLAST of PI0026749 vs. NCBI nr
Match: XP_038899202.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 693.7 bits (1789), Expect = 1.1e-195
Identity = 337/475 (70.95%), Postives = 389/475 (81.89%), Query Frame = 0

Query: 1   MEQPAMITGIILS-LLFFLYTVFVMSRRSVA-QRKTLPPEVDGAWPVIGHLHLLNASEPT 60
           ME+P ++T II + L FF Y +F MSRR V   RK LPPE   AWPVIGHLH LNASEP 
Sbjct: 1   MEEPHVLTVIIFAGLFFFFYVIFTMSRRYVVPHRKRLPPEAGRAWPVIGHLHQLNASEPI 60

Query: 61  HITLAKMADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNY 120
           HITLAKMADAYGPIF FRFG K+ LIVSNWEIAKE FTTND+IFASRPKL ASKLL Y+Y
Sbjct: 61  HITLAKMADAYGPIFTFRFGMKKTLIVSNWEIAKELFTTNDRIFASRPKLLASKLLTYDY 120

Query: 121 CMIGFSAYNPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSE 180
            MIGFS YNPHWRYVRKIAT+GLL NH + QL+ +R LEV+ WMKE+++   L+N ++SE
Sbjct: 121 AMIGFSLYNPHWRYVRKIATLGLLTNHRVEQLEHVRALEVQTWMKEIHELYWLKNKEKSE 180

Query: 181 EVFVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREKFRKALNDFLEFFGRFIPSDSF 240
            V VDMKKWF+DI  N IFKMVIGKRF TAFDD +  +K RKAL DF E FG+FIPSD F
Sbjct: 181 RVVVDMKKWFQDITLNTIFKMVIGKRFTTAFDDDIASKKCRKALRDFSELFGKFIPSDWF 240

Query: 241 SILSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKI-EEQDFMDAVISTVEN 300
             +SWLD  GH + MKKTAQ LD+T H FLQ+HRERRDC+KGK+ +EQDFMD +ISTVE+
Sbjct: 241 PFVSWLDSVGHREEMKKTAQILDKTFHNFLQEHRERRDCSKGKMDQEQDFMDLMISTVED 300

Query: 301 DDGENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGR 360
           D+GE LSY+ D IIKATCLN+I+GG DTT++ +TWALSLLLNNEE LRKAQLE+D+QIGR
Sbjct: 301 DEGEPLSYHVDRIIKATCLNVIIGGFDTTSIIMTWALSLLLNNEEALRKAQLELDKQIGR 360

Query: 361 EREVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQK 420
           ER+VKESDI NL Y+ AIVKETLRL+P  PLLVPHES EDC   GYHIPKGT+LIVN QK
Sbjct: 361 ERQVKESDIKNLSYLQAIVKETLRLYPAAPLLVPHESSEDCTTAGYHIPKGTRLIVNVQK 420

Query: 421 LQKDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAIICSANVI 473
           LQKDP VWEDPC+FRPERFLT   N+D+RG+NP+LIPFGNGRR CPAI  +  +I
Sbjct: 421 LQKDPCVWEDPCKFRPERFLTSHKNIDVRGQNPQLIPFGNGRRQCPAISFALQII 475

BLAST of PI0026749 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 411.8 bits (1057), Expect = 7.8e-115
Identity = 210/470 (44.68%), Postives = 306/470 (65.11%), Query Frame = 0

Query: 10  IILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEP-THITLAKMADA 69
           + + +L F++    + ++S   +    P   GAWP+IGHLHLL   E   + TL KMAD 
Sbjct: 8   LFVPILVFVF--IALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADH 67

Query: 70  YGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNP 129
           YGP    + G     +VS++E+AK+CFT NDK  ASRP   A+K + YN+ + GF+ Y+ 
Sbjct: 68  YGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSA 127

Query: 130 HWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWF 189
            WR +RKIATI LL N  +  L+ +R  E+   +K+L  + L   N  ++ V VD+K W 
Sbjct: 128 FWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDL--YSLWFKNGGTKPVMVDLKSWL 187

Query: 190 EDIIFNIIFKMVIGKRF-----ATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSW 249
           ED+  N+I +MV GKR+     + + +D     + +KA+  F    G F  SD+F  LS+
Sbjct: 188 EDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSF 247

Query: 250 LDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENL 309
            DL GHEK MK+T   LD  L ++++ HR++R  +  K  + DF+D ++S  E     +L
Sbjct: 248 FDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHL 307

Query: 310 SYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKE 369
            Y+A+T IK+TCL +I+GG DT+  T+TWA+SLLLNN+E+L+KAQ EID  +GR+R V++
Sbjct: 308 QYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVED 367

Query: 370 SDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPM 429
           SDI NL+Y+ AI+KETLRL+P GPLL P E++EDC + GY++P GT+LIVN  K+Q+DP 
Sbjct: 368 SDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPK 427

Query: 430 VWEDPCEFRPERFLTRE-NNVDLRGKNPKLIPFGNGRRICPAIICSANVI 473
           V+ +P EFRPERF+T E    D+RG+N +L+PFG+GRR CP    +  V+
Sbjct: 428 VYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVL 473

BLAST of PI0026749 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 397.5 bits (1020), Expect = 1.5e-110
Identity = 202/469 (43.07%), Postives = 302/469 (64.39%), Query Frame = 0

Query: 10  IILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEP-THITLAKMADA 69
           + + +L F++    + ++S   +    P   GAWP+IGHLHLL+  E   + TL KMAD 
Sbjct: 8   LFVPILVFVF--IALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQ 67

Query: 70  YGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNP 129
           YGP    R G     +VS++E+AK+CFT NDK  ASRP   A+K + Y+  + GF+ Y+ 
Sbjct: 68  YGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSA 127

Query: 130 HWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWF 189
            WR +RKIAT+ LL N  +  L+ +R  E+   M++L  + L      SE V VD+K W 
Sbjct: 128 FWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDL--YSLWVKKGGSEPVMVDLKSWL 187

Query: 190 EDIIFNIIFKMVIGKRF----ATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSWL 249
           ED+  N++ +MV GKR+    + + +D     + RK + +F    G F  SD+F  L W 
Sbjct: 188 EDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWF 247

Query: 250 DLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENLS 309
           D  GHEK MK+T + LD  L ++++ HR++R  +  K  + DF+D ++S  E     +L 
Sbjct: 248 DFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQ 307

Query: 310 YNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKES 369
           ++A T IK+TCL +I+GG +T+  T+TWA+SLLLNN+++L+KAQ EID  +GR+R V++S
Sbjct: 308 HDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDS 367

Query: 370 DINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPMV 429
           DI NL+Y+ AI+KETLRL+P GPLL   E+IEDC + GY++ +GT+++VN  K+Q+DP V
Sbjct: 368 DIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRV 427

Query: 430 WEDPCEFRPERFLTRE-NNVDLRGKNPKLIPFGNGRRICPAIICSANVI 473
           + +P EFRPERF+T E    D+RG+N +L+PFG+GRR CP    +  V+
Sbjct: 428 YMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVL 472

BLAST of PI0026749 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 372.5 bits (955), Expect = 5.2e-103
Identity = 200/461 (43.38%), Postives = 288/461 (62.47%), Query Frame = 0

Query: 10  IILSLLFFLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEP-THITLAKMADA 69
           + +S+L F++    + ++S   +    P   GAWP+IGHLHLL   E   + TL KMAD 
Sbjct: 8   LFVSILVFVF--IALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADH 67

Query: 70  YGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAYNP 129
           YGP    R G     + S++E+AK+CFT NDK  AS     A+K + Y +          
Sbjct: 68  YGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGYVF---------- 127

Query: 130 HWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKKWF 189
            W  +RKIA I LL N  +  L  +R  E+   +K+L  + L      SE V VD+K W 
Sbjct: 128 -WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDL--YSLWVKKGGSEPVMVDLKSWL 187

Query: 190 EDIIFNIIFKMVIGKRF-----ATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSW 249
           ED+I N+I +MV GKR+     A + +      ++RK +  F    G F  SD+F  L W
Sbjct: 188 EDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGW 247

Query: 250 LDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENL 309
           LDL GHEK MK+T + LD  L ++++ HR++R  +  K  + DF+D ++S  E     +L
Sbjct: 248 LDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHL 307

Query: 310 SYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKE 369
            Y+A+T IK TCL +I+GG +T+  T+TWA+SLLLNN+++L+K Q EID  +GR+R V++
Sbjct: 308 QYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVED 367

Query: 370 SDINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPM 429
           SDI NL+Y+ AI+KETLRL+P  PLL   E++EDC + GY++P GT+LIVN  K+Q+DP 
Sbjct: 368 SDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPK 427

Query: 430 VWEDPCEFRPERFLTRE-NNVDLRGKNPKLIPFGNGRRICP 464
           V+ +P EFRPERF+T E  + D+RG+N +L+PFG+GRR CP
Sbjct: 428 VYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCP 452

BLAST of PI0026749 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 337.0 bits (863), Expect = 2.4e-92
Identity = 197/475 (41.47%), Postives = 283/475 (59.58%), Query Frame = 0

Query: 6   MITGIILSLLF-FLYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLAK 65
           +I   +LS LF F   + + SR     +K+  P V GAWP++GHLHL +   PTH+T   
Sbjct: 3   LIMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGA 62

Query: 66  MADAYGPIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFS 125
           MAD YGP+FM + G  +V+I+++ E+AKE +T +DK+   RP+L ASKLL YN   + FS
Sbjct: 63  MADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFS 122

Query: 126 AYNPHWRYVRKIATIGLLR----NHHMPQLQKIRELEVR----NWMKELNDFCLLQNNKR 185
            Y  +WR +RKIA   L      + HM    +  +L  R     W K           K 
Sbjct: 123 PYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEK---------RGKP 182

Query: 186 SEEVFVDMKKWFEDIIFNIIFKMVIGKRFATAFDDPVCREK----FRKALNDFLEFFGRF 245
            E V VDMK+ F D+  NI   MV GKR+    ++P C  K      K + +FL++F  F
Sbjct: 183 KEGVLVDMKQEFIDLTANISLMMVSGKRYFG--ENPNCEVKEARRCGKLIREFLDYFALF 242

Query: 246 IPSDSFSILSWLDLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVI 305
           + SD   +L +LD    ++ MK+TA+ LD+    ++++H+ +R  + G+  E D++D +I
Sbjct: 243 LLSDVAPVLGFLD-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRS-DHGR-SENDYLDILI 302

Query: 306 STVENDDGENLSYNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEID 365
             +  D    LS +  T IKA CLN+++ G +T  V + WA+SLLLNN  +LRKAQ E+D
Sbjct: 303 KILGQDKIPGLS-DTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELD 362

Query: 366 EQIGREREVKESDINNLLYVNAIVKETLRLHPPGPLLVPHESIE--DCAIVGYHIPKGTQ 425
            +IG+ER V+E DI +L+Y+ AIVKET RL+PP PL+     +E  D A    H+P GTQ
Sbjct: 363 SKIGKERVVEELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQ 422

Query: 426 LIVNAQKLQKDPMVWEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICPAI 466
           L+V+A K+ +DP VW +P +F PERFLT    +D+ G++ K  PFG GRR CPAI
Sbjct: 423 LMVSAWKIHRDPNVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAI 461

BLAST of PI0026749 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 283.5 bits (724), Expect = 3.2e-76
Identity = 171/459 (37.25%), Postives = 253/459 (55.12%), Query Frame = 0

Query: 10  IILSLLFF-LYTVFVMSRRSVAQRKTLPPEVDGAWPVIGHLHLLNASEPTHITLAKMADA 69
           +I + LF  L   F++ R  + +R  LPP    A PVIGHL LL    P H     ++++
Sbjct: 6   LIFTFLFISLSLTFIIGR--IKRRPNLPPSPSWALPVIGHLRLL--KPPLHRVFLSVSES 65

Query: 70  YG--PIFMFRFGRKRVLIVSNWEIAKECFTTNDKIFASRPKLFASKLLAYNYCMIGFSAY 129
            G  PI   R G + V +VS+  +A+ECFT ND + A+R    ASK ++Y    +  ++Y
Sbjct: 66  LGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASY 125

Query: 130 NPHWRYVRKIATIGLLRNHHMPQLQKIRELEVRNWMKELNDFCLLQNNKRSEEVFVDMKK 189
             HWR +R+I  + +   H +     IR  E+   +        L  N   E   V+MK 
Sbjct: 126 GDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLI------ACLSRNSSLEFTKVEMKS 185

Query: 190 WFEDIIFNIIFKMVIGKRF--ATAFDDPVCREKFRKALNDFLEFFGRFIPSDSFSILSWL 249
            F ++ FN I +M+ GK +    A DDP  + + R+ + + +  FG    +D   IL+W+
Sbjct: 186 MFSNLTFNNIIRMLAGKCYYGDGAEDDPEAK-RVRELIAEGMGCFGAGNTADYLPILTWI 245

Query: 250 DLGGHEKAMKKTAQTLDETLHKFLQQHRERRDCNKGKIEEQDFMDAVISTVENDDGENLS 309
              G EK +KK A  LDE L   + + RE      GK + Q+ M   +  ++    E   
Sbjct: 246 T--GSEKRIKKIASRLDEFLQGLVDERRE------GKEKRQNTMVDHLLCLQETQPE--- 305

Query: 310 YNADTIIKATCLNMIVGGIDTTTVTITWALSLLLNNEEILRKAQLEIDEQIGREREVKES 369
           Y  D IIK   L++I+ G DT+ VT+ W LS LLN+ +IL KA+ EID ++G  R V+ES
Sbjct: 306 YYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEES 365

Query: 370 DINNLLYVNAIVKETLRLHPPGPLLVPHESIEDCAIVGYHIPKGTQLIVNAQKLQKDPMV 429
           D+++L Y+  IV E+LRL+P  PLLVPH + EDC + GYH+P+GT L+ NA  + +DP +
Sbjct: 366 DLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKI 425

Query: 430 WEDPCEFRPERFLTRENNVDLRGKNPKLIPFGNGRRICP 464
           W+DP  F+PERF       +  G+  KL+ FG GRR CP
Sbjct: 426 WDDPTSFKPERF-------EKEGEAQKLLGFGLGRRACP 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498583.4e-11544.75Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
Q9SZ461.1e-11344.68Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... [more]
A0A0N7F2971.0e-11144.73Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... [more]
Q430685.6e-11042.77Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2[more]
O493942.1e-10943.07Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LDQ21.8e-19971.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852620 PE=3 SV=1[more]
A0A1S3CNU63.3e-19870.46cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502576 PE=3 SV=1[more]
A0A6J1F5K68.0e-17664.72cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3... [more]
A0A6J1CCZ41.4e-17563.75cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=... [more]
A0A6J1IFL61.3e-17363.88cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_004147804.13.7e-19971.76cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59913.1 hypothetical protein Csa_... [more]
XP_008464768.16.9e-19870.46PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo][more]
XP_038903603.19.0e-19869.12cytochrome P450 CYP82D47-like [Benincasa hispida][more]
XP_038899739.19.0e-19870.38cytochrome P450 CYP82D47-like [Benincasa hispida][more]
XP_038899202.11.1e-19570.95cytochrome P450 CYP82D47-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G31940.17.8e-11544.68cytochrome P450, family 82, subfamily C, polypeptide 4 [more]
AT4G31970.11.5e-11043.07cytochrome P450, family 82, subfamily C, polypeptide 2 [more]
AT4G31950.15.2e-10343.38cytochrome P450, family 82, subfamily C, polypeptide 3 [more]
AT2G25160.12.4e-9241.47cytochrome P450, family 82, subfamily F, polypeptide 1 [more]
AT4G37340.13.2e-7637.25cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 374..385
score: 45.22
coord: 453..462
score: 58.58
coord: 321..338
score: 37.5
IPR001128Cytochrome P450PFAMPF00067p450coord: 40..463
e-value: 1.9E-88
score: 297.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 191..209
score: 25.42
coord: 66..85
score: 29.21
coord: 452..462
score: 54.62
coord: 310..327
score: 25.78
coord: 373..391
score: 38.02
coord: 414..438
score: 40.38
coord: 90..111
score: 24.7
coord: 330..356
score: 36.91
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 21..466
e-value: 5.8E-110
score: 370.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 42..463
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 4..464
e-value: 1.8E-17
score: 61.2
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 35..473
NoneNo IPR availablePANTHERPTHR47947:SF26CYTOCHROME P450coord: 35..473
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 455..464

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0026749.1PI0026749.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen