PI0026725 (gene) Melon (PI 482460) v1

Overview
NamePI0026725
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAlpha-galactosidase
Locationchr01: 1815782 .. 1818241 (+)
RNA-Seq ExpressionPI0026725
SyntenyPI0026725
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAATTCAGACAAAACTTTGAGGTTACTATTTTATTTGATTTTTATTAGTTCATACCAGCATAATTCATATGTTAGCATAACTTAAATGTAATTAGATGTCTGAGTTTCTTCTCAAGTTCAATTCACTACTACAAAATTGACATTACTTGATGCTTGCAACTTTTAATTATTTGACATTTTTATGAAAAAAATGTCAAGTAATATGTAAATTTTTTTTAAAAAAGGGTCAAGAATAGTAATAAAAAAGTGGAGTTTCACGAAATTTTTAGGATATTTTCATGCTTGCCTAAACTTGACATTTTAAAAAGTCAAGTAATATAAAGTACACAAATTATATAATATTCTCTATATTTTATTTTCCATTCTCTCTCATTTTTGTTGAAAAAACCTACCTCCTTTGCGTGAAAGCAGCCACAAGACACCCATCCCCCATCATCTTCTTCGAACTCTTTCTCACCCTAACTCTTTTCCTCGACCAACCCCTCTCAACGTTGAAAGCAGCCACAACTGCACGATTGTTGCTCTCTCATTCTCTCAACTCTCTCTAGCACTTCCTACTCGAGACATTGCTCCTAGGTCCCTGAGTCCACTTAGGGTTTTGAAAGATGTGATGTCGCTCAATCATCAAATGCGAATAGGTGACATGAATGTTGGGCATTTACGTCTCCACTTAAGGTTTTCATTTTTCCGCTTTTGTTTTACTTTAATTCTAGAGGGAAAAGGGTAGAGTGTCGTGGGTCTCCTAATCAAGTGAAATTTGATTTTGACATCGTATTATTTTGTAGGTTTTTATAAATTCTACTCATGGGATGTGAAAGGCTGTAGCTGTTTAATTTCAGAGATGGGAAGCAGGGCAAAGTTTGAAAAGAAATTAATTCATCTATTGAATGTTTCTGTATTCGATGATGGTTTAATTGTTGTAAATGTAATTAAATTATGTTATTTTGTGATTTCTCTCAAATTTGAATTACTTCGTTAAATTTATCACTTCTTGCTTTCTTTTTTTGGTTTTTGATGGTTATTGATTATGGGTATTTGTTTTGGTTTTAGGATCGGAAAGCGAGTAGTTCATTCACGAAGAGGGGTTCAATGATTTACATAAATATTAATTTATAAGAGCAGCTCAATTATATCGCGATTTGATTCAAGATCTCTTTGAAAGTGAAAATAAAGTGGTTTGTAGCTTTAAAGCCAAATGTGATTTATGCTTCATTTTTTTGTACTATGTAACATGGATAAAGAAAATAACAATTTCAGGATGAAGCCACTGGCTTCACTAATCGTGTGGTGCATATGAACTCTAATTTGTTTCAGAGTGTCCTCACAAACTGGACCAGAACGTGCTGCCCTCCCACCGAGAGGTTTGAACTCCTATGACTCATTTTCTTGGATTATTTCTAAAAAATTCCTAAAAAATGCAGAGATGATTGCCAATCAGCTTAAATCTAAGGGATATGACGTAGGAATTTTTAACTGGCTGACAAATTGTTTTTGTAACATTGGTCATTTTTATGATCTAATGGGTGGATTCCTTCAACTTTATTGTAATGTAATGAAGGTAGTTGGTGTTTCTTGAGAAATTATTCAGTTAGGCCACCACAATGTAGTTGATAGTCTTTCACTATTGAAAAGATTCTTGAAGTTATATTTAGTAGTTATTAAGTGGCATACAAAATTTTGAACTCAAGGGTTGGCAGCTCCATCTTTCCTTTGGCACCAACAACCTGAGGAGTGATGAGACTGGCTAGCAATTATTTTTTCCCCCTCAAGCGGTAAGGTTAGATTTCTTATTTACTAAGGTGTCATATTTGACTGTATTCTATTCAAGAATTTTGATTTCTTGAAGTGTTTTGGGGTTTTAAAACATTGGTTTTCCTTTTCAAAGGTTCTAAAAATTTCTGACTGAATTTGCTGATTGGGGCTTGTTGAAGTGATAGTTTCAAGTTTGTGAGTCCTCATATTGGTACTTATAGGCTCTGATTTGGGTACCCTTTTTCATGTGTATTTTTTGTTTGCATCCAAATTTTGTTGCTTTTGTTTGGTAGTTTAGTGAAATTTAGTCAGGGTTTATTCACTGTAGAGACTGTAGAGAAATAACAAATCCTTGACATTCTTTTCAATAAATAACATGTGCAAACAATCATAGTTTGAACGACTATTTAAAGAGATATGCTAACATATGATGTGCTCCAATTTTAAGCTTGACTTAGAGAAGTATTGTACTTGGAGATAATTTTCTGGTAGATGGTAAGAACTTTCGTTAAGCAACCTACTATGGAAAAAAGGGGTCATTATTGACACCTGAACTTGTACTTATGTGAAGCTTGCGAAGGCAAGATTGATGAAAAGATGTGTAATATCCAACACTCAACCATAATTTATAGCTAATGGAGTACACTTGCATTAGATTCTTTTATTCTTGAGATGCACATTCTTATTTTTTGTAGGATGTAG

mRNA sequence

ATGGAAAAATTCAGACAAAACTTTGAGGGTTTTGAAAGATGTGATGTCGCTCAATCATCAAATGCGAATAGCTGTTTAATTTCAGAGATGGGAAGCAGGGCAAAGTTTGAAAAGAAATTAATTCATCTATTGAATGTTTCTGTATTCGATGATGGTTTAATTGTTGTAAATATCTCTTTGAAAGTGAAAATAAAAGTGTCCTCACAAACTGGACCAGAACGTGCTGCCCTCCCACCGAGAGGTTTGAACTCCTATGACTCATTTTCTTGGATTATTTCTAAAAAATTCCTAAAAAATGCAGAGATGATTGCCAATCAGCTTAAATCTAAGGGATATGACATGCACATTCTTATTTTTTGTAGGATGTAG

Coding sequence (CDS)

ATGGAAAAATTCAGACAAAACTTTGAGGGTTTTGAAAGATGTGATGTCGCTCAATCATCAAATGCGAATAGCTGTTTAATTTCAGAGATGGGAAGCAGGGCAAAGTTTGAAAAGAAATTAATTCATCTATTGAATGTTTCTGTATTCGATGATGGTTTAATTGTTGTAAATATCTCTTTGAAAGTGAAAATAAAAGTGTCCTCACAAACTGGACCAGAACGTGCTGCCCTCCCACCGAGAGGTTTGAACTCCTATGACTCATTTTCTTGGATTATTTCTAAAAAATTCCTAAAAAATGCAGAGATGATTGCCAATCAGCTTAAATCTAAGGGATATGACATGCACATTCTTATTTTTTGTAGGATGTAG

Protein sequence

MEKFRQNFEGFERCDVAQSSNANSCLISEMGSRAKFEKKLIHLLNVSVFDDGLIVVNISLKVKIKVSSQTGPERAALPPRGLNSYDSFSWIISKKFLKNAEMIANQLKSKGYDMHILIFCRM
Homology
[Close]
BLAST of PI0026725 vs. ExPASy TrEMBL
Match: A0A0A0K412 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G063930 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.5e-30
Identity = 87/130 (66.92%), Postives = 93/130 (71.54%), Query Frame = 0 

Query: 1   MEKFRQNFE------GFERCDV-------------AQSSNANSCLISEMGSRAKFEKKLI 60
           MEKFR+NFE      GFER DV             +  +   SCLIS+MGSRAKF KKLI
Sbjct: 1   MEKFRRNFEVCESTWGFERFDVLSHLMRIGFYKFYSWDAIGCSCLISQMGSRAKFGKKLI 60

Query: 61  HLLNVSVFDDGLIVVNISLKVKIK-VSSQTGPERAALPPRGLNSYDSFSWIISKKFLKNA 111
           HLLNVSVF D LIVVN SLKVKIK VSSQTGPE AALPP   NSYD FSWI SK+FL N+
Sbjct: 61  HLLNVSVFSDSLIVVNTSLKVKIKRVSSQTGPECAALPP--WNSYDPFSWISSKEFLTNS 120

[Close]
BLAST of PI0026725 vs. ExPASy TrEMBL
Match: A0A0A0LK91 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_2G349640 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 3.4e-14
Identity = 43/53 (81.13%), Postives = 49/53 (92.45%), Query Frame = 0 

Query: 66  VSSQTGPERAALPPRGLNSYDSFSWIIS-KKFLKNAEMIANQLKSKGYDMHIL 118
           VSSQTGPERAALPPRG NSYDSFSWIIS ++FLKNAE++ANQLKSKGY+  I+
Sbjct: 19  VSSQTGPERAALPPRGWNSYDSFSWIISEEEFLKNAEIVANQLKSKGYEYVIV 71

[Close]
BLAST of PI0026725 vs. ExPASy TrEMBL
Match: A0A1S3B9Q1 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103487727 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.1e-12
Identity = 40/53 (75.47%), Postives = 47/53 (88.68%), Query Frame = 0 

Query: 66  VSSQTGPERAALPPRGLNSYDSFSWIIS-KKFLKNAEMIANQLKSKGYDMHIL 118
           VSSQTGPERAALPPRG NSYDSFSWIIS ++FL N E++AN+LKSKGY+  I+
Sbjct: 19  VSSQTGPERAALPPRGWNSYDSFSWIISEEEFLNNVEIVANKLKSKGYEYVIV 71

[Close]
BLAST of PI0026725 vs. ExPASy TrEMBL
Match: A0A5A7UYS7 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001900 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.1e-12
Identity = 40/53 (75.47%), Postives = 47/53 (88.68%), Query Frame = 0 

Query: 66  VSSQTGPERAALPPRGLNSYDSFSWIIS-KKFLKNAEMIANQLKSKGYDMHIL 118
           VSSQTGPERAALPPRG NSYDSFSWIIS ++FL N E++AN+LKSKGY+  I+
Sbjct: 19  VSSQTGPERAALPPRGWNSYDSFSWIISEEEFLNNVEIVANKLKSKGYEYVIV 71

[Close]
BLAST of PI0026725 vs. ExPASy TrEMBL
Match: A0A5A7SK77 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002350 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.6e-11
Identity = 40/55 (72.73%), Postives = 47/55 (85.45%), Query Frame = 0 

Query: 62  VKIKVSSQTGPERAALPPRGLNSYDSFSWIISKKFLKNAEMIANQLKSKGYDMHI 117
           +K+ VSSQTG ERAALPP    S+DSFSWIISK+FL NAEMIANQLKSKGY++ +
Sbjct: 888 MKVGVSSQTGLERAALPP--WYSHDSFSWIISKEFLTNAEMIANQLKSKGYEVEL 940

[Close]
BLAST of PI0026725 vs. NCBI nr
Match: XP_011649499.1 (uncharacterized protein LOC101206292 [Cucumis sativus] >XP_031736692.1 uncharacterized protein LOC101206292 [Cucumis sativus] >KGN62330.1 hypothetical protein Csa_018547 [Cucumis sativus])

HSP 1 Score: 87.8 bits (216), Expect = 7.0e-14
Identity = 43/53 (81.13%), Postives = 49/53 (92.45%), Query Frame = 0 

Query: 66  VSSQTGPERAALPPRGLNSYDSFSWIIS-KKFLKNAEMIANQLKSKGYDMHIL 118
           VSSQTGPERAALPPRG NSYDSFSWIIS ++FLKNAE++ANQLKSKGY+  I+
Sbjct: 19  VSSQTGPERAALPPRGWNSYDSFSWIISEEEFLKNAEIVANQLKSKGYEYVIV 71

[Close]
BLAST of PI0026725 vs. NCBI nr
Match: KAA0061052.1 (Melibiase domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 82.8 bits (203), Expect = 2.2e-12
Identity = 40/53 (75.47%), Postives = 47/53 (88.68%), Query Frame = 0 

Query: 66  VSSQTGPERAALPPRGLNSYDSFSWIIS-KKFLKNAEMIANQLKSKGYDMHIL 118
           VSSQTGPERAALPPRG NSYDSFSWIIS ++FL N E++AN+LKSKGY+  I+
Sbjct: 19  VSSQTGPERAALPPRGWNSYDSFSWIISEEEFLNNVEIVANKLKSKGYEYVIV 71

[Close]
BLAST of PI0026725 vs. NCBI nr
Match: XP_008444380.1 (PREDICTED: uncharacterized protein LOC103487727 [Cucumis melo] >XP_008444381.1 PREDICTED: uncharacterized protein LOC103487727 [Cucumis melo])

HSP 1 Score: 82.8 bits (203), Expect = 2.2e-12
Identity = 40/53 (75.47%), Postives = 47/53 (88.68%), Query Frame = 0 

Query: 66  VSSQTGPERAALPPRGLNSYDSFSWIIS-KKFLKNAEMIANQLKSKGYDMHIL 118
           VSSQTGPERAALPPRG NSYDSFSWIIS ++FL N E++AN+LKSKGY+  I+
Sbjct: 19  VSSQTGPERAALPPRGWNSYDSFSWIISEEEFLNNVEIVANKLKSKGYEYVIV 71

[Close]
BLAST of PI0026725 vs. NCBI nr
Match: XP_038886461.1 (alpha-galactosidase mel1 [Benincasa hispida])

HSP 1 Score: 80.5 bits (197), Expect = 1.1e-11
Identity = 38/49 (77.55%), Postives = 45/49 (91.84%), Query Frame = 0 

Query: 66  VSSQTGPERAALPPRGLNSYDSFSWIIS-KKFLKNAEMIANQLKSKGYD 114
           VSSQTGPERAALPPRG NSYDSFSWIIS ++FL+NAE++AN+LK  GY+
Sbjct: 25  VSSQTGPERAALPPRGWNSYDSFSWIISEEEFLQNAEIVANRLKPMGYE 73

[Close]
BLAST of PI0026725 vs. NCBI nr
Match: KAA0031472.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK06925.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 79.0 bits (193), Expect = 3.2e-11
Identity = 40/55 (72.73%), Postives = 47/55 (85.45%), Query Frame = 0 

Query: 62  VKIKVSSQTGPERAALPPRGLNSYDSFSWIISKKFLKNAEMIANQLKSKGYDMHI 117
           +K+ VSSQTG ERAALPP    S+DSFSWIISK+FL NAEMIANQLKSKGY++ +
Sbjct: 888 MKVGVSSQTGLERAALPP--WYSHDSFSWIISKEFLTNAEMIANQLKSKGYEVEL 940

[Close]
BLAST of PI0026725 vs. TAIR 10
Match: AT3G26380.1 (Melibiase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 4.0e-07
Identity = 24/60 (40.00%), Postives = 42/60 (70.00%), Query Frame = 0 

Query: 54  IVVNISLKVKIKVSSQTGPERAALPPRGLNSYDSFSWIISK-KFLKNAEMIANQLKSKGY 113
           I+ N+S+   + + +++  + A+ PPRG NSYDSF W IS+ +FL++AE+++ +L   GY
Sbjct: 19  IIFNLSI-FSLSIEARSRQQHASFPPRGWNSYDSFCWTISEAEFLQSAEIVSKRLLPHGY 77

The following BLAST results are available for this feature:
BLAST of PI0026725 vs.
Match NameE-valueIdentityDescription
BLAST of PI0026725 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K4121.5e-3066.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G063930 PE=4 SV=1[more]
A0A0A0LK913.4e-1481.13Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_2G349640 PE=3 SV=1[more]
A0A1S3B9Q11.1e-1275.47Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103487727 PE=3 SV=1[more]
A0A5A7UYS71.1e-1275.47Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G... [more]
A0A5A7SK771.6e-1172.73Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
BLAST of PI0026725 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011649499.17.0e-1481.13uncharacterized protein LOC101206292 [Cucumis sativus] >XP_031736692.1 uncharact... [more]
KAA0061052.12.2e-1275.47Melibiase domain-containing protein [Cucumis melo var. makuwa][more]
XP_008444380.12.2e-1275.47PREDICTED: uncharacterized protein LOC103487727 [Cucumis melo] >XP_008444381.1 P... [more]
XP_038886461.11.1e-1177.55alpha-galactosidase mel1 [Benincasa hispida][more]
KAA0031472.13.2e-1172.73pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK06925... [more]
BLAST of PI0026725 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G26380.14.0e-0740.00Melibiase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 56..120
e-value: 2.7E-5
score: 25.4
NoneNo IPR availablePANTHERPTHR11452:SF42ALPHA-GALACTOSIDASEcoord: 53..114
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 53..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0026725.1PI0026725.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds