PI0026391 (gene) Melon (PI 482460) v1

Overview
NamePI0026391
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionProtein kinase domain-containing protein
Locationchr05: 4188863 .. 4192969 (-)
RNA-Seq ExpressionPI0026391
SyntenyPI0026391
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCTTATTAGAGCTCTCTAAGTGTTGTTGATCTCTGCTGCAAATATTCAGTGATGTCTTCTTCACAAATTCATTTTGTGGTTCATACAAACCATGATTGCTTCTATGGCTTTAGAGGATTCTTGGCTGTGGCAAAAGGATTTCACATGTTGGCCTTGTAATCAACTACAACAACCTCTCCAAGTAAGTGGATCTTGATTCTCTAGAGAGGAAAAAAACCATTTTTTTTTTCCTTTTATCTTGTTTCTTTCGTTTGTAAATTCATACGATCATGAGTCAAGGGCGAGAATAAAATCTGACCTGGTAATGCTTTAGGTACTTTACATATGTTTTTTGACCTATGTCAAATTAAGAGTCGACTCTACGTCCCTACCTGTCACTTATTAAAAATTTGGCTAAATTATATAAAATATTTTTATTCTTTCTAAAGTTGTACTATTATACTTGATTTTTTATGAACAATCCTTAAATTCTTTAGAAAATCAAACAAAAATTGAGACCTGAAATAGTGTGGTGATACAAACTTACATTCTAAGCCATTGTCATACTATTTTATGTCCTAATTTTTAAAAACCAAGAAATTTCTACTCTACAATAATTTTGAAACGTTTATGAAAAGTCAAAGATCATATTGCAACTTTTAAAAGAGCAAAGAAATATGCTTGAAACAAACGACAAAATTCAGAGGTTTTTTTTGTAATTTGACCTGAAATTTTAGAATAATAGTTTTACCGTACGTATTTCAAACAAACAAAAAGGGCTAAAATTTATTTCTCCACATTTGACAGGAATACAGAAGAAAACAAAGGCATGGCCACCAATGATCTAAACAAAGAGCTCTCAAAGAAAACAAACATATTTGGTCTAAAAGTATGGGAAGTCATTGGAATTGGAGTTGGCCTTTTCATAATCTCAATCCTCTGTATTCTCTCACTTTGTTTAACCTCATTCAACAAGAAATCAAAAAAATCATCATCACCCAAATTTCCTCTTACTCAAATCCCATCTCATTCAAAGGACATAAAGGCAATTCATATAACAACTACTCACAAAAAGCCAACAACAACCCAAATGGGAAAATTTGAAACCAATAATAATTACAGTGGTGAATCAGGATCATTGTTTCATCCATTGAGTTATGGCTATGGATCACAATCAGGGGATGAAGGCAGCTCGGGAACTGTCACGACAACAACAATGTACCGACGTTCGACGTCACCTACGACAACAACAATGACTGCTCCTTCGCCGTTGTTGGGTATGCCAGAGATGTCGCAATTGGGGTGGGGGTATTGGTTTACTTTAAGAGAGCTGGATTTGGCTACTAATTTGTTCTCAGAAGAGAATTTGATTGGAGAAGGAGGGTATGGAGTTGTTTTTAGAGGAAGGTTGTTGAATGGGACACCAGTTGCAGTTAAGAAGATTTTTAATGGACAGTAAGTTGTTATTTTTAAAAGAATGATAGTAAATAGAACTATGCATAGTGTCGTAGTACTAGTAATTTTTTGGAAAAATTTCTAGTGTTATGATAGTACCCATCTGCATATTCTCGTTCATGATTTGACACCTCATCAAACTATATATTAATTTATTATTCATTTATTTTAATAAAAAGTAAATTTTTGGTGTTGGGAGTTTTAGAAATTTTCTAGTATTCTTTTTAATATTAGTGGATACCACGTTAATTTTAAAAGAATGATGACATGACAAGCTGTAATAAATACTAGTATGTATGATATCATAATACTAGTAATTTTTTTTAAGAAACTTCTAGTGCTATGACAATATCTACTACTCTTATTCATGATTTGATACGTCATTGATTGAAATACTAATTTATTTTGTAGACCCCATTGATCTCTTTCACATTTTGTTATTGTTTGCAATTCTTTAAAAGTGTTGTTATACGCTTAATTATTAGTTTTAAAAGTGCTAGCAATTGCAATTACCATAAATTATACTTTTAGTGATAGTCAATGAATAAAGATGTACGTAGTACTAGAAATTTTCTCAACTTTTTGAAGGGTAAATTTGACCTTTTTTTTTTTGTAGGGGCCAAGCGGAGAAAGAATTTAGAGCGGAAGTTGAGGCAATTGGGCATGTAAGACATAAGAATCTCGTTCGTCTTTTGGGATATTGCATTGAAGGGACTCATAGGTATATCTTACGTACCTAACCATTAGTTCTAGAAAAGGAAAAAAAGTGGGTGTACGAACCCTCCTGTTGCATCCAATTAAAAATTGTCATGTGACTGACATTTTATTTTTTTAAAAAAAATATTATAATTATTTTTTGTGTAGTAGAAGGGAGAGTAGTTGAAAATATTTACGCGATTTCTCTATTTGAAATAATTTTTCTAAAAAGAATTTAACGGTGGACTTAGTTCAATGCTGACCTAAAAATAAATATCAGTTAGCAGAGATATATGATTCGAAATAATTATAGAGTGTACAATATTTTTAGCTTTTGTTCCACACTCTTTAGGATGTTGGTATATGAATACATAAACAATGGAAGCTTAGAGCTATGGCTACATGAAGGAATGGGAGAGAATACATATCTTACATGGGAGGCTAGAATGAAGATCATGTTGGGGACAGCCAAAGGGTTAGTAAAATCAAGTTCAATTTAATTTCAAGTTTTTGAGAACATGATCATCTCTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTGTTTTAAACAACCTATGATTATTAATTATTTTTGTCATGTCACAAAAGAATTGCATACTTACATGAAGCCATTGAGCCAAAGGTGGTGCATAGAGACATAAAGGCTAGCAACATACTTGTAGATGAAAATTTCAATGCTAAAGTATCAGATTTTGGGTTAGCAAAGTTGATGGATGCTAACAAAACTCATGTCACAACAAGAGTTATGGGCACATTTGGGTATAATTCTTCTATATATATATATATATATATATATTTATTCCCTTTTGGATCTATAATATATTATATAGTTTTATATTGTTGTGTGGTTCGGATCTATATACTATAGTTATGTCGCTCCTGAATATGCAAACACCGGACTTCTAAATGAGAAGAGCGATGTGTATAGTTTTGGAGTTGTGTTGGTGGAAACAATCACAGGAAGAGATCCAGTAGACTATGGACGTCCACCCAAACAGGTAGGTTTGCCAATCATTCTAATAATTTCTTGATCCATGGATGGTTTTTTTAGGGTTGAGGCTATTTAATCCCATGTGTACTTTGAAAAGTTTCTAATAGGTTTGTGAAATTTTAATTTTAAGGCTTATGTACTGTTGATGTGGATGTATCATTACAGACAAAAAAAATGTTAAATTACAATTTTAATCCTCCCAAACTTTAAAATGAGTGTTTATTTGGTCTCTAAGCTTTAATAAATATTTAATTAAGTCTTTCAACTTTCAATTTTATGTCTAATAGATTTCAATAGTATATATTAAATTTTATTAAAAAATGTTGTGTTTAATTAGACATTCAAAACTTTTAGTTTTGTGTATCTATAGAATATATCTAATCCTTTTTCTCATGTTCTAATTTTCATATTTGTCAAAGGAAAAAAAAAAAAAGAAAAGAAACTTAGTGATGAGTAGAAAGAAATAGTAACATTCGTAATATTTAAAGATACAAATGTGTTGTTTTAGAACAAAGATAACGAGATATTACTTAAACGTACGACATTGATTAGAATATATTTTTTTTCAAAGTATAGGAACTAAAATAAATGGGATTATTATTTGTAGGTGAACCTGGTGGATTGGTTAAAAATGATGATTGGAAGTAGGAGATGTGAAGAAGTGGTAGACCCAAAAATAGGGGTTGGAGGAAGGCCATCCACAAGAGGGTTGAAAAGAGTTCTATTGGTTGCATTGAGATGTGTTGATCCTGATTTTGAAAAGAGGCCAAAAATGGGCCAAGTTGTTAGAATGCTTGAATCTCAAGATCATCATCATTATAATTCTTCTCATACACCTCCTACTACTACCACTTGACTTTTCTTTCTTTCTTTCTTTAATTTTACATCTCTAACTTCTTTTTATGTATAGGATATTTGATTCACATTCACATTATTGGTTTATAAAATATATATTTGTGTC

mRNA sequence

CTTCTTCTTATTAGAGCTCTCTAAGTGTTGTTGATCTCTGCTGCAAATATTCAGTGATGTCTTCTTCACAAATTCATTTTGTGGTTCATACAAACCATGATTGCTTCTATGGCTTTAGAGGATTCTTGGCTGTGGCAAAAGGATTTCACATGTTGGCCTTGTAATCAACTACAACAACCTCTCCAAGAATACAGAAGAAAACAAAGGCATGGCCACCAATGATCTAAACAAAGAGCTCTCAAAGAAAACAAACATATTTGGTCTAAAAGTATGGGAAGTCATTGGAATTGGAGTTGGCCTTTTCATAATCTCAATCCTCTGTATTCTCTCACTTTGTTTAACCTCATTCAACAAGAAATCAAAAAAATCATCATCACCCAAATTTCCTCTTACTCAAATCCCATCTCATTCAAAGGACATAAAGGCAATTCATATAACAACTACTCACAAAAAGCCAACAACAACCCAAATGGGAAAATTTGAAACCAATAATAATTACAGTGGTGAATCAGGATCATTGTTTCATCCATTGAGTTATGGCTATGGATCACAATCAGGGGATGAAGGCAGCTCGGGAACTGTCACGACAACAACAATGTACCGACGTTCGACGTCACCTACGACAACAACAATGACTGCTCCTTCGCCGTTGTTGGGTATGCCAGAGATGTCGCAATTGGGGTGGGGGTATTGGTTTACTTTAAGAGAGCTGGATTTGGCTACTAATTTGTTCTCAGAAGAGAATTTGATTGGAGAAGGAGGGTATGGAGTTGTTTTTAGAGGAAGGTTGTTGAATGGGACACCAGTTGCAGTTAAGAAGATTTTTAATGGACAGGGCCAAGCGGAGAAAGAATTTAGAGCGGAAGTTGAGGCAATTGGGCATGTAAGACATAAGAATCTCGTTCGTCTTTTGGGATATTGCATTGAAGGGACTCATAGGATGTTGGTATATGAATACATAAACAATGGAAGCTTAGAGCTATGGCTACATGAAGGAATGGGAGAGAATACATATCTTACATGGGAGGCTAGAATGAAGATCATGTTGGGGACAGCCAAAGGAATTGCATACTTACATGAAGCCATTGAGCCAAAGGTGGTGCATAGAGACATAAAGGCTAGCAACATACTTGTAGATGAAAATTTCAATGCTAAAGTATCAGATTTTGGGTTAGCAAAGTTGATGGATGCTAACAAAACTCATGTCACAACAAGAGTTATGGGCACATTTGGTTATGTCGCTCCTGAATATGCAAACACCGGACTTCTAAATGAGAAGAGCGATGTGTATAGTTTTGGAGTTGTGTTGGTGGAAACAATCACAGGAAGAGATCCAGTAGACTATGGACGTCCACCCAAACAGGTGAACCTGGTGGATTGGTTAAAAATGATGATTGGAAGTAGGAGATGTGAAGAAGTGGTAGACCCAAAAATAGGGGTTGGAGGAAGGCCATCCACAAGAGGGTTGAAAAGAGTTCTATTGGTTGCATTGAGATGTGTTGATCCTGATTTTGAAAAGAGGCCAAAAATGGGCCAAGTTGTTAGAATGCTTGAATCTCAAGATCATCATCATTATAATTCTTCTCATACACCTCCTACTACTACCACTTGACTTTTCTTTCTTTCTTTCTTTAATTTTACATCTCTAACTTCTTTTTATGTATAGGATATTTGATTCACATTCACATTATTGGTTTATAAAATATATATTTGTGTC

Coding sequence (CDS)

ATGGCCACCAATGATCTAAACAAAGAGCTCTCAAAGAAAACAAACATATTTGGTCTAAAAGTATGGGAAGTCATTGGAATTGGAGTTGGCCTTTTCATAATCTCAATCCTCTGTATTCTCTCACTTTGTTTAACCTCATTCAACAAGAAATCAAAAAAATCATCATCACCCAAATTTCCTCTTACTCAAATCCCATCTCATTCAAAGGACATAAAGGCAATTCATATAACAACTACTCACAAAAAGCCAACAACAACCCAAATGGGAAAATTTGAAACCAATAATAATTACAGTGGTGAATCAGGATCATTGTTTCATCCATTGAGTTATGGCTATGGATCACAATCAGGGGATGAAGGCAGCTCGGGAACTGTCACGACAACAACAATGTACCGACGTTCGACGTCACCTACGACAACAACAATGACTGCTCCTTCGCCGTTGTTGGGTATGCCAGAGATGTCGCAATTGGGGTGGGGGTATTGGTTTACTTTAAGAGAGCTGGATTTGGCTACTAATTTGTTCTCAGAAGAGAATTTGATTGGAGAAGGAGGGTATGGAGTTGTTTTTAGAGGAAGGTTGTTGAATGGGACACCAGTTGCAGTTAAGAAGATTTTTAATGGACAGGGCCAAGCGGAGAAAGAATTTAGAGCGGAAGTTGAGGCAATTGGGCATGTAAGACATAAGAATCTCGTTCGTCTTTTGGGATATTGCATTGAAGGGACTCATAGGATGTTGGTATATGAATACATAAACAATGGAAGCTTAGAGCTATGGCTACATGAAGGAATGGGAGAGAATACATATCTTACATGGGAGGCTAGAATGAAGATCATGTTGGGGACAGCCAAAGGAATTGCATACTTACATGAAGCCATTGAGCCAAAGGTGGTGCATAGAGACATAAAGGCTAGCAACATACTTGTAGATGAAAATTTCAATGCTAAAGTATCAGATTTTGGGTTAGCAAAGTTGATGGATGCTAACAAAACTCATGTCACAACAAGAGTTATGGGCACATTTGGTTATGTCGCTCCTGAATATGCAAACACCGGACTTCTAAATGAGAAGAGCGATGTGTATAGTTTTGGAGTTGTGTTGGTGGAAACAATCACAGGAAGAGATCCAGTAGACTATGGACGTCCACCCAAACAGGTGAACCTGGTGGATTGGTTAAAAATGATGATTGGAAGTAGGAGATGTGAAGAAGTGGTAGACCCAAAAATAGGGGTTGGAGGAAGGCCATCCACAAGAGGGTTGAAAAGAGTTCTATTGGTTGCATTGAGATGTGTTGATCCTGATTTTGAAAAGAGGCCAAAAATGGGCCAAGTTGTTAGAATGCTTGAATCTCAAGATCATCATCATTATAATTCTTCTCATACACCTCCTACTACTACCACTTGA

Protein sequence

MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFPLTQIPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGYGSQSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGRPSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYNSSHTPPTTTT
Homology
[Close]
BLAST of PI0026391 vs. ExPASy Swiss-Prot
Match: Q9SJG2 (Probable receptor-like protein kinase At2g42960 OS=Arabidopsis thaliana OX=3702 GN=At2g42960 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.5e-152
Identity = 284/468 (60.68%), Postives = 352/468 (75.21%), Query Frame = 0 

Query: 4   NDLNKELSKKTNIF---GLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 63
           + LN E+SKK + F   GLK+W  + + VG+FI+ ILCILSL +T F +KS++SSS KFP
Sbjct: 5   SSLNAEMSKKISFFGLKGLKLWVWVCLVVGVFIVMILCILSLWIT-FRRKSRRSSS-KFP 64

Query: 64  LTQIPSHSKDIK------------AIHITTTHKKPTTTQM---GKFETNNNYSGESGSLF 123
             QIP  SKDI+            +++I    K    T M   G+ ++++N +    S  
Sbjct: 65  FNQIPHVSKDIRVDRAGFQNPHPESLYIEMNDKSTGKTMMSHLGRTKSSDNDTLSQCSSV 124

Query: 124 HPLSYGYGSQSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTL 183
           +       S SG+EG  G+       +    P T      SPL+G+PE+S LGWG+WFTL
Sbjct: 125 NHHERACSSHSGEEGGFGSAGR----QYGGGPVTA-----SPLVGLPEISHLGWGHWFTL 184

Query: 184 RELDLATNLFSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGH 243
           R+L+LATN F+  N++GEGGYGVV+RG+L+NGT VAVKK+ N  GQAEKEFR EVEAIGH
Sbjct: 185 RDLELATNRFAPVNVLGEGGYGVVYRGKLVNGTEVAVKKLLNNLGQAEKEFRVEVEAIGH 244

Query: 244 VRHKNLVRLLGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKG 303
           VRHKNLVRLLGYCIEG HRMLVYEY+N+G+LE WLH  M ++  LTWEARMKI+ GTA+ 
Sbjct: 245 VRHKNLVRLLGYCIEGVHRMLVYEYVNSGNLEQWLHGAMRQHGNLTWEARMKIITGTAQA 304

Query: 304 IAYLHEAIEPKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVA 363
           +AYLHEAIEPKVVHRDIKASNIL+D+ FNAK+SDFGLAKL+D+ ++H+TTRVMGTFGYVA
Sbjct: 305 LAYLHEAIEPKVVHRDIKASNILIDDEFNAKLSDFGLAKLLDSGESHITTRVMGTFGYVA 364

Query: 364 PEYANTGLLNEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVV 423
           PEYANTGLLNEKSD+YSFGV+L+E ITGRDPVDYGRP  +VNLV+WLKMM+G+RR EEVV
Sbjct: 365 PEYANTGLLNEKSDIYSFGVLLLEAITGRDPVDYGRPANEVNLVEWLKMMVGTRRAEEVV 424

Query: 424 DPKIGVGGRPSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           DP++    RPS   LKR LLV+LRCVDP+ EKRP+M QV RMLES +H
Sbjct: 425 DPRL--EPRPSKSALKRALLVSLRCVDPEAEKRPRMSQVARMLESDEH 459

[Close]
BLAST of PI0026391 vs. ExPASy Swiss-Prot
Match: Q8LEB6 (Probable receptor-like protein kinase At5g18500 OS=Arabidopsis thaliana OX=3702 GN=At5g18500 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.0e-140
Identity = 264/453 (58.28%), Postives = 330/453 (72.85%), Query Frame = 0 

Query: 6   LNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFPLTQ-- 65
           LN  LS+  N  GL++WE+I I +    + +L I SL LT F +K+ +SSS   P+++  
Sbjct: 5   LNDTLSRNYN--GLELWEIIVIVLSAIFVVVLAI-SLWLT-FRRKTSRSSSNLIPVSRQI 64

Query: 66  IPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNY---SGESGSLFHPLSYGYGSQSGDEG 125
            PS  ++IK I +          ++      N Y   S + G           S++GD  
Sbjct: 65  PPSVPEEIKEIRV---------DEVSSSNGGNGYPSISEKFGDKEPEKGIKAESENGDSS 124

Query: 126 SSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSEENL 185
            SG+         S+  +   +TAPSPL G+PE S LGWG+WFTLR+L +ATN FS +N+
Sbjct: 125 RSGSFNHLEKKDGSSVSSANPLTAPSPLSGLPEFSHLGWGHWFTLRDLQMATNQFSRDNI 184

Query: 186 IGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGYCIE 245
           IG+GGYGVV+RG L+NGTPVAVKK+ N  GQA+K+FR EVEAIGHVRHKNLVRLLGYC+E
Sbjct: 185 IGDGGYGVVYRGNLVNGTPVAVKKLLNNLGQADKDFRVEVEAIGHVRHKNLVRLLGYCME 244

Query: 246 GTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKVVHR 305
           GT RMLVYEY+NNG+LE WL      + YLTWEAR+KI++GTAK +AYLHEAIEPKVVHR
Sbjct: 245 GTQRMLVYEYVNNGNLEQWLRGDNQNHEYLTWEARVKILIGTAKALAYLHEAIEPKVVHR 304

Query: 306 DIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEKSDV 365
           DIK+SNIL+D+ FN+K+SDFGLAKL+ A+K+ +TTRVMGTFGYVAPEYAN+GLLNEKSDV
Sbjct: 305 DIKSSNILIDDKFNSKISDFGLAKLLGADKSFITTRVMGTFGYVAPEYANSGLLNEKSDV 364

Query: 366 YSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGRPSTRGL 425
           YSFGVVL+E ITGR PVDY RPP +V+LV+WLKMM+  RR EEVVDP +    +PST  L
Sbjct: 365 YSFGVVLLEAITGRYPVDYARPPPEVHLVEWLKMMVQQRRSEEVVDPNLET--KPSTSAL 424

Query: 426 KRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           KR LL ALRCVDP  EKRP+M QV RMLES+++
Sbjct: 425 KRTLLTALRCVDPMSEKRPRMSQVARMLESEEY 442

[Close]
BLAST of PI0026391 vs. ExPASy Swiss-Prot
Match: Q9LRP3 (Probable receptor-like protein kinase At3g17420 OS=Arabidopsis thaliana OX=3702 GN=At3g17420 PE=1 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.5e-139
Identity = 257/453 (56.73%), Postives = 341/453 (75.28%), Query Frame = 0 

Query: 3   TNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFPLT 62
           T+ L + L+K+  +  L++WE+I I +    I IL +LS+ L SF KKSK+S++   P+T
Sbjct: 2   TSQLKRTLTKRYGV--LELWEIIVIALFAAFIVIL-VLSVWL-SFRKKSKRSNATTLPVT 61

Query: 63  QIPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGY--GSQSGDEG 122
           Q P  +++IK I +                 ++  S  +G+ +  L   +    ++GD+ 
Sbjct: 62  QSPRFTEEIKEISV-----------------DHGSSNNNGTSYQTLDEKFVEDIENGDK- 121

Query: 123 SSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSEENL 182
            SG++    +      P+T + TAPSPLLG+PE+S +GWG+WFTLR+L LATN FS+E++
Sbjct: 122 FSGSLEKKPLVGSHLPPSTPSTTAPSPLLGLPEVSHIGWGHWFTLRDLQLATNHFSKESI 181

Query: 183 IGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGYCIE 242
           IG+GGYGVV+ G L N TPVAVKK+ N  GQA+K+FR EVEAIGHVRHKNLVRLLGYC+E
Sbjct: 182 IGDGGYGVVYHGTLTNKTPVAVKKLLNNPGQADKDFRVEVEAIGHVRHKNLVRLLGYCVE 241

Query: 243 GTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKVVHR 302
           GTHRMLVYEY+NNG+LE WLH  M    +LTWEAR+K+++GTAK +AYLHEAIEPKVVHR
Sbjct: 242 GTHRMLVYEYMNNGNLEQWLHGDMIHKGHLTWEARIKVLVGTAKALAYLHEAIEPKVVHR 301

Query: 303 DIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEKSDV 362
           DIK+SNIL+D+NF+AK+SDFGLAKL+ A+  +V+TRVMGTFGYVAPEYAN+GLLNEKSDV
Sbjct: 302 DIKSSNILMDDNFDAKLSDFGLAKLLGADSNYVSTRVMGTFGYVAPEYANSGLLNEKSDV 361

Query: 363 YSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGRPSTRGL 422
           YS+GVVL+E ITGR PVDY RP ++V++V+WLK+M+  ++ EEVVD ++ +  +P+T  L
Sbjct: 362 YSYGVVLLEAITGRYPVDYARPKEEVHMVEWLKLMVQQKQFEEVVDKELEI--KPTTSEL 421

Query: 423 KRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           KR LL ALRCVDPD +KRPKM QV RMLES ++
Sbjct: 422 KRALLTALRCVDPDADKRPKMSQVARMLESDEY 430

[Close]
BLAST of PI0026391 vs. ExPASy Swiss-Prot
Match: Q3EDL4 (Probable serine/threonine-protein kinase At1g01540 OS=Arabidopsis thaliana OX=3702 GN=At1g01540 PE=1 SV=2)

HSP 1 Score: 479.9 bits (1234), Expect = 3.2e-134
Identity = 248/447 (55.48%), Postives = 314/447 (70.25%), Query Frame = 0 

Query: 6   LNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFPLTQIP 65
           LN ELSK T+IFGL++W VIGI +G  I+  L +LSLCLTS  K  K  +         P
Sbjct: 9   LNTELSKPTSIFGLRLWVVIGILLGSLIVIALFLLSLCLTSRRKNRKPRADFASAAIATP 68

Query: 66  SHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGYGSQSGDEGSSGTV 125
             SK+IK I        P   Q+           + G + H + +     SG+   + + 
Sbjct: 69  PISKEIKEIVPAQNQSVPAEIQV-----------DIGKIEHRVVFSDRVSSGESRGTASA 128

Query: 126 TTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSEENLIGEGG 185
           + T  Y  S +               PE+S LGWG W+TLREL+ ATN   EEN+IGEGG
Sbjct: 129 SETASYSGSGNCG-------------PEVSHLGWGRWYTLRELEAATNGLCEENVIGEGG 188

Query: 186 YGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGYCIEGTHRM 245
           YG+V+RG L +GT VAVK + N +GQAEKEF+ EVE IG VRHKNLVRLLGYC+EG +RM
Sbjct: 189 YGIVYRGILTDGTKVAVKNLLNNRGQAEKEFKVEVEVIGRVRHKNLVRLLGYCVEGAYRM 248

Query: 246 LVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKVVHRDIKAS 305
           LVY++++NG+LE W+H  +G+ + LTW+ RM I+LG AKG+AYLHE +EPKVVHRDIK+S
Sbjct: 249 LVYDFVDNGNLEQWIHGDVGDVSPLTWDIRMNIILGMAKGLAYLHEGLEPKVVHRDIKSS 308

Query: 306 NILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEKSDVYSFGV 365
           NIL+D  +NAKVSDFGLAKL+ +  ++VTTRVMGTFGYVAPEYA TG+LNEKSD+YSFG+
Sbjct: 309 NILLDRQWNAKVSDFGLAKLLGSESSYVTTRVMGTFGYVAPEYACTGMLNEKSDIYSFGI 368

Query: 366 VLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGRPSTRGLKRVLL 425
           +++E ITGR+PVDY RP  + NLVDWLK M+G+RR EEVVDPKI     PS++ LKRVLL
Sbjct: 369 LIMEIITGRNPVDYSRPQGETNLVDWLKSMVGNRRSEEVVDPKI--PEPPSSKALKRVLL 428

Query: 426 VALRCVDPDFEKRPKMGQVVRMLESQD 453
           VALRCVDPD  KRPKMG ++ MLE++D
Sbjct: 429 VALRCVDPDANKRPKMGHIIHMLEAED 429

[Close]
BLAST of PI0026391 vs. ExPASy Swiss-Prot
Match: Q6NKZ9 (Probable receptor-like serine/threonine-protein kinase At4g34500 OS=Arabidopsis thaliana OX=3702 GN=At4g34500 PE=2 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.0e-116
Identity = 229/452 (50.66%), Postives = 303/452 (67.04%), Query Frame = 0 

Query: 7   NKELSKKTNIFGLKVWEVIGI-GVGLFIISILCILSLCLTSFNKKSK---KSSSPKFPLT 66
           +K  + K ++FGL ++ VI I  V + +IS+L  L +CL   ++  +   K SS   PL 
Sbjct: 9   HKSSTTKPSVFGLNLYLVIAICSVFILLISLLIFLFVCLNRVSRARRMRVKHSSGSIPLV 68

Query: 67  QIPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGYGSQSGDEGSS 126
                SK+I  I             +GKF  +++  G+                   G+ 
Sbjct: 69  -----SKEISEI-----------KTVGKFINSDDSKGKI------------------GNE 128

Query: 127 GTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSEENLIG 186
             V  +   + +TS   T   A S  +G  E   +GWG W++L++L++AT  FS++N+IG
Sbjct: 129 VVVVVSATSKEATSGFDTLSVASSGDVGTSE--AMGWGKWYSLKDLEIATRGFSDDNMIG 188

Query: 187 EGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGYCIEG- 246
           EGGYGVV+R    +G+  AVK + N +GQAEKEF+ EVEAIG VRHKNLV L+GYC +  
Sbjct: 189 EGGYGVVYRADFSDGSVAAVKNLLNNKGQAEKEFKVEVEAIGKVRHKNLVGLMGYCADSA 248

Query: 247 -THRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKVVHR 306
            + RMLVYEYI+NG+LE WLH  +G  + LTW+ RMKI +GTAKG+AYLHE +EPKVVHR
Sbjct: 249 QSQRMLVYEYIDNGNLEQWLHGDVGPVSPLTWDIRMKIAIGTAKGLAYLHEGLEPKVVHR 308

Query: 307 DIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEKSDV 366
           D+K+SNIL+D+ +NAKVSDFGLAKL+ +  ++VTTRVMGTFGYV+PEYA+TG+LNE SDV
Sbjct: 309 DVKSSNILLDKKWNAKVSDFGLAKLLGSETSYVTTRVMGTFGYVSPEYASTGMLNECSDV 368

Query: 367 YSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGRPSTRGL 426
           YSFGV+L+E ITGR PVDY RPP ++NLVDW K M+ SRR EEV+DPKI     P  R L
Sbjct: 369 YSFGVLLMEIITGRSPVDYSRPPGEMNLVDWFKGMVASRRGEEVIDPKIKTS--PPPRAL 422

Query: 427 KRVLLVALRCVDPDFEKRPKMGQVVRMLESQD 453
           KR LLV LRC+D D  KRPKMGQ++ MLE++D
Sbjct: 429 KRALLVCLRCIDLDSSKRPKMGQIIHMLEAED 422

[Close]
BLAST of PI0026391 vs. ExPASy TrEMBL
Match: A0A5A7UR31 (Putative receptor-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00650 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.6e-256
Identity = 447/461 (96.96%), Postives = 454/461 (98.48%), Query Frame = 0 

Query: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 60
           MATNDLNKELSKKTNIFGLKVWEV+GIGVG+FIISILCILSLCLTSFNKKSKKSS PKFP
Sbjct: 1   MATNDLNKELSKKTNIFGLKVWEVVGIGVGVFIISILCILSLCLTSFNKKSKKSSPPKFP 60

Query: 61  LTQIPSHSKDIKAIHITTT--HKKPTTTQMGKFET-NNNYSGESGSLFHPLSYGYGSQSG 120
           LTQIPSHSKDIKAIHITTT  HKKPTTTQ GKFET NNNYSGESGSLFHPLSYGYGSQSG
Sbjct: 61  LTQIPSHSKDIKAIHITTTTDHKKPTTTQTGKFETNNNNYSGESGSLFHPLSYGYGSQSG 120

Query: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 180
           DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPL GMPEMSQLGWGYWFTLRELDLATNLFSE
Sbjct: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLSGMPEMSQLGWGYWFTLRELDLATNLFSE 180

Query: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240
           ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY
Sbjct: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240

Query: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300
           CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV
Sbjct: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300

Query: 301 VHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360
           VHRDIKASNIL+DENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK
Sbjct: 301 VHRDIKASNILIDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360

Query: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPK-IGVGGRPS 420
           SDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPK IGVGGRPS
Sbjct: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIIGVGGRPS 420

Query: 421 TRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYN 458
           TRGLKRVLLVALRCVDPDF+KRPKMGQVVRMLESQDHHH++
Sbjct: 421 TRGLKRVLLVALRCVDPDFDKRPKMGQVVRMLESQDHHHHS 461

[Close]
BLAST of PI0026391 vs. ExPASy TrEMBL
Match: A0A1S3BVM8 (probable receptor-like protein kinase At2g42960 OS=Cucumis melo OX=3656 GN=LOC103493978 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.4e-254
Identity = 445/461 (96.53%), Postives = 452/461 (98.05%), Query Frame = 0 

Query: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 60
           MATNDLNKELSKKTNIFGLKVWEV+GIGVG+FIISILCILSLCLTSFNKKSKKSS PKFP
Sbjct: 1   MATNDLNKELSKKTNIFGLKVWEVVGIGVGVFIISILCILSLCLTSFNKKSKKSSPPKFP 60

Query: 61  LTQIPSHSKDIKAIHITTT--HKKPTTTQMGKFET-NNNYSGESGSLFHPLSYGYGSQSG 120
           LTQIPSHSKDIKAIHITTT  HKKPTTTQ GKFET NNNYSGESGSLFHPLSYGYGSQSG
Sbjct: 61  LTQIPSHSKDIKAIHITTTTDHKKPTTTQTGKFETNNNNYSGESGSLFHPLSYGYGSQSG 120

Query: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 180
           DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPL GMPEMSQL WGYWFTLRELDLATNLFSE
Sbjct: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLSGMPEMSQLRWGYWFTLRELDLATNLFSE 180

Query: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240
           ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY
Sbjct: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240

Query: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300
           CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV
Sbjct: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300

Query: 301 VHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360
           VHRDIKASNIL+DENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYA TGLLNEK
Sbjct: 301 VHRDIKASNILIDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYAYTGLLNEK 360

Query: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPK-IGVGGRPS 420
           SDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPK IGVGGRPS
Sbjct: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIIGVGGRPS 420

Query: 421 TRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYN 458
           TRGLKRVLLVALRCVDPDF+KRPKMGQVVRMLESQDHHH++
Sbjct: 421 TRGLKRVLLVALRCVDPDFDKRPKMGQVVRMLESQDHHHHS 461

[Close]
BLAST of PI0026391 vs. ExPASy TrEMBL
Match: A0A0A0LGK4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034780 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.6e-251
Identity = 443/458 (96.72%), Postives = 450/458 (98.25%), Query Frame = 0 

Query: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 60
           MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSS PKFP
Sbjct: 43  MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSPPKFP 102

Query: 61  LTQIPSHSKDIKAIHITTT-HKKPTTTQMGKFET-NNNYSGES-GSLFHPLSYGYGSQSG 120
           LTQIPSHSKDIKAIHITTT HKKPTTTQMGKFE+ NN+YSGES GSLF+PLSYGYGSQSG
Sbjct: 103 LTQIPSHSKDIKAIHITTTDHKKPTTTQMGKFESHNNDYSGESGGSLFYPLSYGYGSQSG 162

Query: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 180
           DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE
Sbjct: 163 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 222

Query: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240
           ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY
Sbjct: 223 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 282

Query: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300
           CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWE+RMKIMLGTAKGIAYLHEAIEPKV
Sbjct: 283 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWESRMKIMLGTAKGIAYLHEAIEPKV 342

Query: 301 VHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360
           VHRDIKASNIL+DENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK
Sbjct: 343 VHRDIKASNILIDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 402

Query: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPK-IGVGGRPS 420
           SDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPK IGVGGR S
Sbjct: 403 SDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIIGVGGRAS 462

Query: 421 TRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHH 455
           TRGLKRVLLVALRCVDPDF+KRPKMGQV RMLESQ HH
Sbjct: 463 TRGLKRVLLVALRCVDPDFDKRPKMGQVARMLESQHHH 500

[Close]
BLAST of PI0026391 vs. ExPASy TrEMBL
Match: A0A6J1KHF8 (probable receptor-like protein kinase At2g42960 OS=Cucurbita maxima OX=3661 GN=LOC111493892 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.7e-210
Identity = 383/469 (81.66%), Postives = 402/469 (85.71%), Query Frame = 0 

Query: 4   NDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPK----- 63
           NDLN+ELSK+T+I GLKVWE+IGIGVGLFIISILCILS  LTS NK S+ SSS       
Sbjct: 6   NDLNQELSKRTSILGLKVWEIIGIGVGLFIISILCILSFFLTSRNKNSRTSSSAANANTN 65

Query: 64  ----FPLTQIPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGYGS 123
                PLTQIPSHSKDIKA+HITT                  YS ESGS FHPLSYGYGS
Sbjct: 66  TNVLCPLTQIPSHSKDIKAVHITT------------------YSTESGSSFHPLSYGYGS 125

Query: 124 QSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNL 183
           QSGDEGSSGTVTTTTM RRST          SP+ G+ EMS+LGWGYWFTLRELDLATNL
Sbjct: 126 QSGDEGSSGTVTTTTMNRRST----------SPMSGLLEMSRLGWGYWFTLRELDLATNL 185

Query: 184 FSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRL 243
           FSEENLIGEGGYGVV++GRLLNG  VAVKKIFNGQGQAE EFR EVEAIGHVRHKNLVRL
Sbjct: 186 FSEENLIGEGGYGVVYKGRLLNGISVAVKKIFNGQGQAEMEFRVEVEAIGHVRHKNLVRL 245

Query: 244 LGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIE 303
           LGYC+EGTHRMLVYEYINNGSLELWLHEGMGEN YLTWEARMKIMLGTAKGIAYLHEAIE
Sbjct: 246 LGYCVEGTHRMLVYEYINNGSLELWLHEGMGENRYLTWEARMKIMLGTAKGIAYLHEAIE 305

Query: 304 PKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLL 363
           PKVVHRDIKASNIL+DENFNAKVSDFGLAKLM+  KTHVTTRVMGTFGYVAPEYANTGLL
Sbjct: 306 PKVVHRDIKASNILIDENFNAKVSDFGLAKLMEVGKTHVTTRVMGTFGYVAPEYANTGLL 365

Query: 364 NEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGR 423
           NEKSDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPKIG+ GR
Sbjct: 366 NEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIGIEGR 425

Query: 424 PSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYNSSHTPP 464
           PSTRGLKR LLVALRCVDP++EKRPKM QVVRMLESQD    + SHT P
Sbjct: 426 PSTRGLKRALLVALRCVDPNYEKRPKMSQVVRMLESQD----SFSHTRP 442

[Close]
BLAST of PI0026391 vs. ExPASy TrEMBL
Match: A0A6J1G2U6 (probable receptor-like protein kinase At2g42960 OS=Cucurbita moschata OX=3662 GN=LOC111450312 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.4e-209
Identity = 384/465 (82.58%), Postives = 400/465 (86.02%), Query Frame = 0 

Query: 4   NDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPK----- 63
           NDLN+ELSK T+I GLKVWEVIGIGVGLFIISILCILS  LTS NK S+ SSS       
Sbjct: 6   NDLNQELSKTTSILGLKVWEVIGIGVGLFIISILCILSFFLTSSNKNSRTSSSAANANTN 65

Query: 64  --FPLTQIPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGYGSQS 123
              PLTQIPSHSKDIKA+HITT                  YS ESGS F PLSYGYGSQS
Sbjct: 66  TLCPLTQIPSHSKDIKAVHITT------------------YSTESGSSFPPLSYGYGSQS 125

Query: 124 GDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFS 183
           GDEGSSGTVTTTTM RRST          SPL G+ EMS+LGWGYWFTLRELDLATNLFS
Sbjct: 126 GDEGSSGTVTTTTMNRRST----------SPLSGLLEMSRLGWGYWFTLRELDLATNLFS 185

Query: 184 EENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLG 243
           EENLIGEGGYGVV++GRLLNG  VAVKKIFNGQGQAE EFR EVEAIGHVRHKNLVRLLG
Sbjct: 186 EENLIGEGGYGVVYKGRLLNGISVAVKKIFNGQGQAEMEFRVEVEAIGHVRHKNLVRLLG 245

Query: 244 YCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPK 303
           YCIEGT RMLVYEYINNGSLELWLHEGMGEN YLTWEARMKIMLGTAKG+AYLHEAIEPK
Sbjct: 246 YCIEGTDRMLVYEYINNGSLELWLHEGMGENRYLTWEARMKIMLGTAKGLAYLHEAIEPK 305

Query: 304 VVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNE 363
           VVHRDIKASNIL+DENFNAKVSDFGLAKLM+  KTHVTTRVMGTFGYVAPEYANTGLLNE
Sbjct: 306 VVHRDIKASNILIDENFNAKVSDFGLAKLMEVGKTHVTTRVMGTFGYVAPEYANTGLLNE 365

Query: 364 KSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGRPS 423
           KSDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGS+RCEEVVDPKIG+ GRPS
Sbjct: 366 KSDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSKRCEEVVDPKIGIEGRPS 425

Query: 424 TRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYNSSHT 462
           TRGLKRVLLVALRCVDPD+EKRPKM QVVRMLESQD    +SSHT
Sbjct: 426 TRGLKRVLLVALRCVDPDYEKRPKMSQVVRMLESQD----SSSHT 438

[Close]
BLAST of PI0026391 vs. NCBI nr
Match: KAA0056085.1 (putative receptor-like protein kinase [Cucumis melo var. makuwa] >TYJ96432.1 putative receptor-like protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 892.5 bits (2305), Expect = 1.6e-255
Identity = 447/461 (96.96%), Postives = 454/461 (98.48%), Query Frame = 0 

Query: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 60
           MATNDLNKELSKKTNIFGLKVWEV+GIGVG+FIISILCILSLCLTSFNKKSKKSS PKFP
Sbjct: 1   MATNDLNKELSKKTNIFGLKVWEVVGIGVGVFIISILCILSLCLTSFNKKSKKSSPPKFP 60

Query: 61  LTQIPSHSKDIKAIHITTT--HKKPTTTQMGKFET-NNNYSGESGSLFHPLSYGYGSQSG 120
           LTQIPSHSKDIKAIHITTT  HKKPTTTQ GKFET NNNYSGESGSLFHPLSYGYGSQSG
Sbjct: 61  LTQIPSHSKDIKAIHITTTTDHKKPTTTQTGKFETNNNNYSGESGSLFHPLSYGYGSQSG 120

Query: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 180
           DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPL GMPEMSQLGWGYWFTLRELDLATNLFSE
Sbjct: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLSGMPEMSQLGWGYWFTLRELDLATNLFSE 180

Query: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240
           ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY
Sbjct: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240

Query: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300
           CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV
Sbjct: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300

Query: 301 VHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360
           VHRDIKASNIL+DENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK
Sbjct: 301 VHRDIKASNILIDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360

Query: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPK-IGVGGRPS 420
           SDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPK IGVGGRPS
Sbjct: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIIGVGGRPS 420

Query: 421 TRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYN 458
           TRGLKRVLLVALRCVDPDF+KRPKMGQVVRMLESQDHHH++
Sbjct: 421 TRGLKRVLLVALRCVDPDFDKRPKMGQVVRMLESQDHHHHS 461

[Close]
BLAST of PI0026391 vs. NCBI nr
Match: XP_008453191.1 (PREDICTED: probable receptor-like protein kinase At2g42960 [Cucumis melo])

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-253
Identity = 445/461 (96.53%), Postives = 452/461 (98.05%), Query Frame = 0 

Query: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 60
           MATNDLNKELSKKTNIFGLKVWEV+GIGVG+FIISILCILSLCLTSFNKKSKKSS PKFP
Sbjct: 1   MATNDLNKELSKKTNIFGLKVWEVVGIGVGVFIISILCILSLCLTSFNKKSKKSSPPKFP 60

Query: 61  LTQIPSHSKDIKAIHITTT--HKKPTTTQMGKFET-NNNYSGESGSLFHPLSYGYGSQSG 120
           LTQIPSHSKDIKAIHITTT  HKKPTTTQ GKFET NNNYSGESGSLFHPLSYGYGSQSG
Sbjct: 61  LTQIPSHSKDIKAIHITTTTDHKKPTTTQTGKFETNNNNYSGESGSLFHPLSYGYGSQSG 120

Query: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 180
           DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPL GMPEMSQL WGYWFTLRELDLATNLFSE
Sbjct: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLSGMPEMSQLRWGYWFTLRELDLATNLFSE 180

Query: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240
           ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY
Sbjct: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240

Query: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300
           CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV
Sbjct: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300

Query: 301 VHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360
           VHRDIKASNIL+DENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYA TGLLNEK
Sbjct: 301 VHRDIKASNILIDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYAYTGLLNEK 360

Query: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPK-IGVGGRPS 420
           SDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPK IGVGGRPS
Sbjct: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIIGVGGRPS 420

Query: 421 TRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYN 458
           TRGLKRVLLVALRCVDPDF+KRPKMGQVVRMLESQDHHH++
Sbjct: 421 TRGLKRVLLVALRCVDPDFDKRPKMGQVVRMLESQDHHHHS 461

[Close]
BLAST of PI0026391 vs. NCBI nr
Match: XP_011648881.1 (probable receptor-like protein kinase At2g42960 isoform X1 [Cucumis sativus])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 443/458 (96.72%), Postives = 450/458 (98.25%), Query Frame = 0 

Query: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 60
           MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSS PKFP
Sbjct: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSPPKFP 60

Query: 61  LTQIPSHSKDIKAIHITTT-HKKPTTTQMGKFET-NNNYSGES-GSLFHPLSYGYGSQSG 120
           LTQIPSHSKDIKAIHITTT HKKPTTTQMGKFE+ NN+YSGES GSLF+PLSYGYGSQSG
Sbjct: 61  LTQIPSHSKDIKAIHITTTDHKKPTTTQMGKFESHNNDYSGESGGSLFYPLSYGYGSQSG 120

Query: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 180
           DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE
Sbjct: 121 DEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLFSE 180

Query: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240
           ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY
Sbjct: 181 ENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLLGY 240

Query: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEPKV 300
           CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWE+RMKIMLGTAKGIAYLHEAIEPKV
Sbjct: 241 CIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWESRMKIMLGTAKGIAYLHEAIEPKV 300

Query: 301 VHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360
           VHRDIKASNIL+DENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK
Sbjct: 301 VHRDIKASNILIDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLNEK 360

Query: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPK-IGVGGRPS 420
           SDVYSFGVVLVETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPK IGVGGR S
Sbjct: 361 SDVYSFGVVLVETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIIGVGGRAS 420

Query: 421 TRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHH 455
           TRGLKRVLLVALRCVDPDF+KRPKMGQV RMLESQ HH
Sbjct: 421 TRGLKRVLLVALRCVDPDFDKRPKMGQVARMLESQHHH 458

[Close]
BLAST of PI0026391 vs. NCBI nr
Match: XP_038890627.1 (probable receptor-like protein kinase At2g42960 [Benincasa hispida])

HSP 1 Score: 865.5 bits (2235), Expect = 2.0e-247
Identity = 439/471 (93.21%), Postives = 449/471 (95.33%), Query Frame = 0 

Query: 1   MATNDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFN-KKSKK----SS 60
           MATNDLNKELSKKTNI GLKVWEVIGIGVGLFIISILCILSLCLTSFN KKSKK    +S
Sbjct: 1   MATNDLNKELSKKTNILGLKVWEVIGIGVGLFIISILCILSLCLTSFNHKKSKKPSAANS 60

Query: 61  SPKFPLTQIPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGYGSQ 120
           +  FPLTQIPSHSKDIKA+HITTT K   TTQMGKFET NNYS ESGSLFHPLSYG+GSQ
Sbjct: 61  NTLFPLTQIPSHSKDIKAVHITTTQKIKPTTQMGKFET-NNYSSESGSLFHPLSYGHGSQ 120

Query: 121 SGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLF 180
           SGDEGSSGTVTTTTMYRRS SPTTT  TAPSPLLGMPEMSQLGWGYWFTLRELDLATNLF
Sbjct: 121 SGDEGSSGTVTTTTMYRRSMSPTTTMTTAPSPLLGMPEMSQLGWGYWFTLRELDLATNLF 180

Query: 181 SEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLL 240
           SEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLL
Sbjct: 181 SEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRLL 240

Query: 241 GYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIEP 300
           GYCIEGTHRMLVYEYINNGSL+LWLHEGMGEN YLTWEARMKIMLGTAKGIAYLHEAIEP
Sbjct: 241 GYCIEGTHRMLVYEYINNGSLDLWLHEGMGENRYLTWEARMKIMLGTAKGIAYLHEAIEP 300

Query: 301 KVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLLN 360
           KVVHRDIKASNIL+DENFNAKVSDFGLAKLMDA KTHVTTRVMGTFGYVAPEYANTGLLN
Sbjct: 301 KVVHRDIKASNILIDENFNAKVSDFGLAKLMDAGKTHVTTRVMGTFGYVAPEYANTGLLN 360

Query: 361 EKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGRP 420
           EKSDVYSFGVVL+ETITGRDPVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPKIGV GRP
Sbjct: 361 EKSDVYSFGVVLIETITGRDPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIGVEGRP 420

Query: 421 STRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYNSSHTPPTTT 467
           STRGLKR+LLVALRCVDPDF+KRPKMGQVVRMLESQD HHYNSSHTP TTT
Sbjct: 421 STRGLKRILLVALRCVDPDFDKRPKMGQVVRMLESQD-HHYNSSHTPSTTT 469

[Close]
BLAST of PI0026391 vs. NCBI nr
Match: XP_023545275.1 (probable receptor-like protein kinase At2g42960 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 747.3 bits (1928), Expect = 8.1e-212
Identity = 387/467 (82.87%), Postives = 403/467 (86.30%), Query Frame = 0 

Query: 4   NDLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPK----- 63
           NDLN+ELSKKT+I GLKVWEVIGIGVGLFIIS LCILS  LTS NK S+ SSS       
Sbjct: 6   NDLNQELSKKTSILGLKVWEVIGIGVGLFIISTLCILSFFLTSRNKNSRTSSSAANTNTN 65

Query: 64  ----FPLTQIPSHSKDIKAIHITTTHKKPTTTQMGKFETNNNYSGESGSLFHPLSYGYGS 123
                PLTQIPSHSKDIKA+HITT                  YS ESGS FHPLSYGYGS
Sbjct: 66  TNTLCPLTQIPSHSKDIKAVHITT------------------YSTESGSSFHPLSYGYGS 125

Query: 124 QSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTLRELDLATNL 183
           QSGDEGSSGTVTTTTM RRST          SPL G+ EMS+LGWGYWFTLRELDLATNL
Sbjct: 126 QSGDEGSSGTVTTTTMNRRST----------SPLSGLLEMSRLGWGYWFTLRELDLATNL 185

Query: 184 FSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGHVRHKNLVRL 243
           FSEENLIGEGGYGVV++GRLLNG  VAVKKIFNGQGQAE EFR EVEAIGHVRHKNLVRL
Sbjct: 186 FSEENLIGEGGYGVVYKGRLLNGISVAVKKIFNGQGQAEMEFRVEVEAIGHVRHKNLVRL 245

Query: 244 LGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKGIAYLHEAIE 303
           LGYC+EGTHRMLVYEYINNGSLELWLHEGMGEN YLTWEARMKIMLGTAKGIAYLHEAIE
Sbjct: 246 LGYCVEGTHRMLVYEYINNGSLELWLHEGMGENRYLTWEARMKIMLGTAKGIAYLHEAIE 305

Query: 304 PKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVAPEYANTGLL 363
           PKVVHRDIKASNIL+DENFNAKVSDFGLAKLM+  KTHVTTRVMGTFGYVAPEYANTGLL
Sbjct: 306 PKVVHRDIKASNILIDENFNAKVSDFGLAKLMEVGKTHVTTRVMGTFGYVAPEYANTGLL 365

Query: 364 NEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVVDPKIGVGGR 423
           NEKSDVYSFGVVLVETITGR+PVDYGRPPKQVN+VDWLKMMIGSRRCEEVVDPKIG+GGR
Sbjct: 366 NEKSDVYSFGVVLVETITGRNPVDYGRPPKQVNVVDWLKMMIGSRRCEEVVDPKIGIGGR 425

Query: 424 PSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDHHHYNSSHT 462
           PSTRGLKRVLLVALRCVDPD+EKRPKM QVVRMLESQD    +SSHT
Sbjct: 426 PSTRGLKRVLLVALRCVDPDYEKRPKMSQVVRMLESQD----SSSHT 440

[Close]
BLAST of PI0026391 vs. TAIR 10
Match: AT1G56720.1 (Protein kinase superfamily protein )

HSP 1 Score: 555.8 bits (1431), Expect = 3.2e-158
Identity = 290/470 (61.70%), Postives = 352/470 (74.89%), Query Frame = 0 

Query: 5   DLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFPLTQI 64
           DL  +LS+++++FGLKVWEVIGI V L II+IL +LS CLTS  KK  + S    P+ Q 
Sbjct: 4   DLKSQLSRESHVFGLKVWEVIGIAVALLIIAILSVLSCCLTS--KKKSRRSKTGLPVIQT 63

Query: 65  P----SHSKDIKAIHITTTHKKPTTTQMGKFETNNN-----------------YSGESGS 124
           P       ++++  H++ ++  P    +   +  NN                  SG SGS
Sbjct: 64  PPVVSKEIREVRVEHVSASNFAPGEGILLTIQDKNNKDSEKVMVHLDMRKKRSSSGRSGS 123

Query: 125 LFHPLSYGYGSQSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWF 184
             H       S S +E S+    ++++Y  +T         PSPL G+PE S LGWG+WF
Sbjct: 124 FHHLEIIDKHSDSAEEVSA----SSSLYNIAT---------PSPLSGLPE-SHLGWGHWF 183

Query: 185 TLRELDLATNLFSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAI 244
           TLR+L+ ATN FS+EN+IGEGGYGVV+RG L+NGTPVAVKKI N  GQAEKEFR EV+AI
Sbjct: 184 TLRDLETATNRFSKENVIGEGGYGVVYRGELMNGTPVAVKKILNQLGQAEKEFRVEVDAI 243

Query: 245 GHVRHKNLVRLLGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTA 304
           GHVRHKNLVRLLGYCIEGTHR+LVYEY+NNG+LE WLH  M ++ YLTWEARMK+++GT+
Sbjct: 244 GHVRHKNLVRLLGYCIEGTHRILVYEYVNNGNLEQWLHGAMRQHGYLTWEARMKVLIGTS 303

Query: 305 KGIAYLHEAIEPKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGY 364
           K +AYLHEAIEPKVVHRDIK+SNIL+++ FNAKVSDFGLAKL+ A K+HVTTRVMGTFGY
Sbjct: 304 KALAYLHEAIEPKVVHRDIKSSNILINDEFNAKVSDFGLAKLLGAGKSHVTTRVMGTFGY 363

Query: 365 VAPEYANTGLLNEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEE 424
           VAPEYAN+GLLNEKSDVYSFGVVL+E ITGRDPVDYGRP  +VNLVDWLKMM+G+RR EE
Sbjct: 364 VAPEYANSGLLNEKSDVYSFGVVLLEAITGRDPVDYGRPAHEVNLVDWLKMMVGTRRSEE 423

Query: 425 VVDPKIGVGGRPSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           VVDP I V  +P TR LKR LL ALRCVDPD +KRPKM QVVRMLES+++
Sbjct: 424 VVDPNIEV--KPPTRSLKRALLTALRCVDPDSDKRPKMSQVVRMLESEEY 455

[Close]
BLAST of PI0026391 vs. TAIR 10
Match: AT1G56720.2 (Protein kinase superfamily protein )

HSP 1 Score: 555.8 bits (1431), Expect = 3.2e-158
Identity = 290/470 (61.70%), Postives = 352/470 (74.89%), Query Frame = 0 

Query: 5   DLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFPLTQI 64
           DL  +LS+++++FGLKVWEVIGI V L II+IL +LS CLTS  KK  + S    P+ Q 
Sbjct: 4   DLKSQLSRESHVFGLKVWEVIGIAVALLIIAILSVLSCCLTS--KKKSRRSKTGLPVIQT 63

Query: 65  P----SHSKDIKAIHITTTHKKPTTTQMGKFETNNN-----------------YSGESGS 124
           P       ++++  H++ ++  P    +   +  NN                  SG SGS
Sbjct: 64  PPVVSKEIREVRVEHVSASNFAPGEGILLTIQDKNNKDSEKVMVHLDMRKKRSSSGRSGS 123

Query: 125 LFHPLSYGYGSQSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWF 184
             H       S S +E S+    ++++Y  +T         PSPL G+PE S LGWG+WF
Sbjct: 124 FHHLEIIDKHSDSAEEVSA----SSSLYNIAT---------PSPLSGLPE-SHLGWGHWF 183

Query: 185 TLRELDLATNLFSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAI 244
           TLR+L+ ATN FS+EN+IGEGGYGVV+RG L+NGTPVAVKKI N  GQAEKEFR EV+AI
Sbjct: 184 TLRDLETATNRFSKENVIGEGGYGVVYRGELMNGTPVAVKKILNQLGQAEKEFRVEVDAI 243

Query: 245 GHVRHKNLVRLLGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTA 304
           GHVRHKNLVRLLGYCIEGTHR+LVYEY+NNG+LE WLH  M ++ YLTWEARMK+++GT+
Sbjct: 244 GHVRHKNLVRLLGYCIEGTHRILVYEYVNNGNLEQWLHGAMRQHGYLTWEARMKVLIGTS 303

Query: 305 KGIAYLHEAIEPKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGY 364
           K +AYLHEAIEPKVVHRDIK+SNIL+++ FNAKVSDFGLAKL+ A K+HVTTRVMGTFGY
Sbjct: 304 KALAYLHEAIEPKVVHRDIKSSNILINDEFNAKVSDFGLAKLLGAGKSHVTTRVMGTFGY 363

Query: 365 VAPEYANTGLLNEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEE 424
           VAPEYAN+GLLNEKSDVYSFGVVL+E ITGRDPVDYGRP  +VNLVDWLKMM+G+RR EE
Sbjct: 364 VAPEYANSGLLNEKSDVYSFGVVLLEAITGRDPVDYGRPAHEVNLVDWLKMMVGTRRSEE 423

Query: 425 VVDPKIGVGGRPSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           VVDP I V  +P TR LKR LL ALRCVDPD +KRPKM QVVRMLES+++
Sbjct: 424 VVDPNIEV--KPPTRSLKRALLTALRCVDPDSDKRPKMSQVVRMLESEEY 455

[Close]
BLAST of PI0026391 vs. TAIR 10
Match: AT1G56720.3 (Protein kinase superfamily protein )

HSP 1 Score: 555.8 bits (1431), Expect = 3.2e-158
Identity = 290/470 (61.70%), Postives = 352/470 (74.89%), Query Frame = 0 

Query: 5   DLNKELSKKTNIFGLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFPLTQI 64
           DL  +LS+++++FGLKVWEVIGI V L II+IL +LS CLTS  KK  + S    P+ Q 
Sbjct: 4   DLKSQLSRESHVFGLKVWEVIGIAVALLIIAILSVLSCCLTS--KKKSRRSKTGLPVIQT 63

Query: 65  P----SHSKDIKAIHITTTHKKPTTTQMGKFETNNN-----------------YSGESGS 124
           P       ++++  H++ ++  P    +   +  NN                  SG SGS
Sbjct: 64  PPVVSKEIREVRVEHVSASNFAPGEGILLTIQDKNNKDSEKVMVHLDMRKKRSSSGRSGS 123

Query: 125 LFHPLSYGYGSQSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWF 184
             H       S S +E S+    ++++Y  +T         PSPL G+PE S LGWG+WF
Sbjct: 124 FHHLEIIDKHSDSAEEVSA----SSSLYNIAT---------PSPLSGLPE-SHLGWGHWF 183

Query: 185 TLRELDLATNLFSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAI 244
           TLR+L+ ATN FS+EN+IGEGGYGVV+RG L+NGTPVAVKKI N  GQAEKEFR EV+AI
Sbjct: 184 TLRDLETATNRFSKENVIGEGGYGVVYRGELMNGTPVAVKKILNQLGQAEKEFRVEVDAI 243

Query: 245 GHVRHKNLVRLLGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTA 304
           GHVRHKNLVRLLGYCIEGTHR+LVYEY+NNG+LE WLH  M ++ YLTWEARMK+++GT+
Sbjct: 244 GHVRHKNLVRLLGYCIEGTHRILVYEYVNNGNLEQWLHGAMRQHGYLTWEARMKVLIGTS 303

Query: 305 KGIAYLHEAIEPKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGY 364
           K +AYLHEAIEPKVVHRDIK+SNIL+++ FNAKVSDFGLAKL+ A K+HVTTRVMGTFGY
Sbjct: 304 KALAYLHEAIEPKVVHRDIKSSNILINDEFNAKVSDFGLAKLLGAGKSHVTTRVMGTFGY 363

Query: 365 VAPEYANTGLLNEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEE 424
           VAPEYAN+GLLNEKSDVYSFGVVL+E ITGRDPVDYGRP  +VNLVDWLKMM+G+RR EE
Sbjct: 364 VAPEYANSGLLNEKSDVYSFGVVLLEAITGRDPVDYGRPAHEVNLVDWLKMMVGTRRSEE 423

Query: 425 VVDPKIGVGGRPSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           VVDP I V  +P TR LKR LL ALRCVDPD +KRPKM QVVRMLES+++
Sbjct: 424 VVDPNIEV--KPPTRSLKRALLTALRCVDPDSDKRPKMSQVVRMLESEEY 455

[Close]
BLAST of PI0026391 vs. TAIR 10
Match: AT2G42960.1 (Protein kinase superfamily protein )

HSP 1 Score: 540.8 bits (1392), Expect = 1.1e-153
Identity = 284/468 (60.68%), Postives = 352/468 (75.21%), Query Frame = 0 

Query: 4   NDLNKELSKKTNIF---GLKVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 63
           + LN E+SKK + F   GLK+W  + + VG+FI+ ILCILSL +T F +KS++SSS KFP
Sbjct: 5   SSLNAEMSKKISFFGLKGLKLWVWVCLVVGVFIVMILCILSLWIT-FRRKSRRSSS-KFP 64

Query: 64  LTQIPSHSKDIK------------AIHITTTHKKPTTTQM---GKFETNNNYSGESGSLF 123
             QIP  SKDI+            +++I    K    T M   G+ ++++N +    S  
Sbjct: 65  FNQIPHVSKDIRVDRAGFQNPHPESLYIEMNDKSTGKTMMSHLGRTKSSDNDTLSQCSSV 124

Query: 124 HPLSYGYGSQSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYWFTL 183
           +       S SG+EG  G+       +    P T      SPL+G+PE+S LGWG+WFTL
Sbjct: 125 NHHERACSSHSGEEGGFGSAGR----QYGGGPVTA-----SPLVGLPEISHLGWGHWFTL 184

Query: 184 RELDLATNLFSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEAIGH 243
           R+L+LATN F+  N++GEGGYGVV+RG+L+NGT VAVKK+ N  GQAEKEFR EVEAIGH
Sbjct: 185 RDLELATNRFAPVNVLGEGGYGVVYRGKLVNGTEVAVKKLLNNLGQAEKEFRVEVEAIGH 244

Query: 244 VRHKNLVRLLGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGTAKG 303
           VRHKNLVRLLGYCIEG HRMLVYEY+N+G+LE WLH  M ++  LTWEARMKI+ GTA+ 
Sbjct: 245 VRHKNLVRLLGYCIEGVHRMLVYEYVNSGNLEQWLHGAMRQHGNLTWEARMKIITGTAQA 304

Query: 304 IAYLHEAIEPKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFGYVA 363
           +AYLHEAIEPKVVHRDIKASNIL+D+ FNAK+SDFGLAKL+D+ ++H+TTRVMGTFGYVA
Sbjct: 305 LAYLHEAIEPKVVHRDIKASNILIDDEFNAKLSDFGLAKLLDSGESHITTRVMGTFGYVA 364

Query: 364 PEYANTGLLNEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCEEVV 423
           PEYANTGLLNEKSD+YSFGV+L+E ITGRDPVDYGRP  +VNLV+WLKMM+G+RR EEVV
Sbjct: 365 PEYANTGLLNEKSDIYSFGVLLLEAITGRDPVDYGRPANEVNLVEWLKMMVGTRRAEEVV 424

Query: 424 DPKIGVGGRPSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           DP++    RPS   LKR LLV+LRCVDP+ EKRP+M QV RMLES +H
Sbjct: 425 DPRL--EPRPSKSALKRALLVSLRCVDPEAEKRPRMSQVARMLESDEH 459

[Close]
BLAST of PI0026391 vs. TAIR 10
Match: AT3G59110.1 (Protein kinase superfamily protein )

HSP 1 Score: 536.6 bits (1381), Expect = 2.0e-152
Identity = 278/471 (59.02%), Postives = 351/471 (74.52%), Query Frame = 0 

Query: 4   NDLNKELSKKTNIFGL---KVWEVIGIGVGLFIISILCILSLCLTSFNKKSKKSSSPKFP 63
           + L+ ++SKK +  GL   K+W +I + VG F++ + CILSL + +F +KS++SS    P
Sbjct: 5   SSLSADMSKKVSFLGLKGMKLWVLICLVVGTFVVLVFCILSLWI-AFRRKSRRSSHKLLP 64

Query: 64  LTQIPSHSKDIKA------------IHITTTHKK------PTTTQMGKFETNNNYSGESG 123
            +QIP  +KDI+             + IT   K          + +G+ ++++N S    
Sbjct: 65  FSQIPRVAKDIRVDDRVGFQNHNENLSITNADKSSDRNSGKMMSYLGRTKSSDNDSISQC 124

Query: 124 SLFHPLSYGYGSQSGDEGSSGTVTTTTMYRRSTSPTTTTMTAPSPLLGMPEMSQLGWGYW 183
           S  H       S SG++GS G         R  S +   +   SPL+G+PE+S LGWG+W
Sbjct: 125 SSVHHHERACSSHSGEDGSFGAAW------RQNSLSQGGLVTASPLVGLPEISHLGWGHW 184

Query: 184 FTLRELDLATNLFSEENLIGEGGYGVVFRGRLLNGTPVAVKKIFNGQGQAEKEFRAEVEA 243
           FTLR+L LATN F+ EN+IGEGGYGVV++GRL+NG  VAVKK+ N  GQAEKEFR EVEA
Sbjct: 185 FTLRDLQLATNRFAAENVIGEGGYGVVYKGRLINGNDVAVKKLLNNLGQAEKEFRVEVEA 244

Query: 244 IGHVRHKNLVRLLGYCIEGTHRMLVYEYINNGSLELWLHEGMGENTYLTWEARMKIMLGT 303
           IGHVRHKNLVRLLGYCIEG +RMLVYEY+N+G+LE WLH  MG+ + LTWEARMKI++GT
Sbjct: 245 IGHVRHKNLVRLLGYCIEGVNRMLVYEYVNSGNLEQWLHGAMGKQSTLTWEARMKILVGT 304

Query: 304 AKGIAYLHEAIEPKVVHRDIKASNILVDENFNAKVSDFGLAKLMDANKTHVTTRVMGTFG 363
           A+ +AYLHEAIEPKVVHRDIKASNIL+D++FNAK+SDFGLAKL+D+ ++H+TTRVMGTFG
Sbjct: 305 AQALAYLHEAIEPKVVHRDIKASNILIDDDFNAKLSDFGLAKLLDSGESHITTRVMGTFG 364

Query: 364 YVAPEYANTGLLNEKSDVYSFGVVLVETITGRDPVDYGRPPKQVNLVDWLKMMIGSRRCE 423
           YVAPEYANTGLLNEKSD+YSFGV+L+ETITGRDPVDY RP  +VNLV+WLKMM+G+RR E
Sbjct: 365 YVAPEYANTGLLNEKSDIYSFGVLLLETITGRDPVDYERPANEVNLVEWLKMMVGTRRAE 424

Query: 424 EVVDPKIGVGGRPSTRGLKRVLLVALRCVDPDFEKRPKMGQVVRMLESQDH 454
           EVVD +I     P+TR LKR LLVALRCVDP+ +KRPKM QVVRMLES +H
Sbjct: 425 EVVDSRI--EPPPATRALKRALLVALRCVDPEAQKRPKMSQVVRMLESDEH 466

The following BLAST results are available for this feature:
BLAST of PI0026391 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SJG21.5e-15260.68Probable receptor-like protein kinase At2g42960 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8LEB63.0e-14058.28Probable receptor-like protein kinase At5g18500 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LRP31.5e-13956.73Probable receptor-like protein kinase At3g17420 OS=Arabidopsis thaliana OX=3702 ... [more]
Q3EDL43.2e-13455.48Probable serine/threonine-protein kinase At1g01540 OS=Arabidopsis thaliana OX=37... [more]
Q6NKZ93.0e-11650.66Probable receptor-like serine/threonine-protein kinase At4g34500 OS=Arabidopsis ... [more]
BLAST of PI0026391 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UR317.6e-25696.96Putative receptor-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BVM85.4e-25496.53probable receptor-like protein kinase At2g42960 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LGK45.6e-25196.72Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034... [more]
A0A6J1KHF81.7e-21081.66probable receptor-like protein kinase At2g42960 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1G2U62.4e-20982.58probable receptor-like protein kinase At2g42960 OS=Cucurbita moschata OX=3662 GN... [more]
BLAST of PI0026391 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0056085.11.6e-25596.96putative receptor-like protein kinase [Cucumis melo var. makuwa] >TYJ96432.1 put... [more]
XP_008453191.11.1e-25396.53PREDICTED: probable receptor-like protein kinase At2g42960 [Cucumis melo][more]
XP_011648881.11.2e-25096.72probable receptor-like protein kinase At2g42960 isoform X1 [Cucumis sativus][more]
XP_038890627.12.0e-24793.21probable receptor-like protein kinase At2g42960 [Benincasa hispida][more]
XP_023545275.18.1e-21282.87probable receptor-like protein kinase At2g42960 [Cucurbita pepo subsp. pepo][more]
BLAST of PI0026391 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G56720.13.2e-15861.70Protein kinase superfamily protein [more]
AT1G56720.23.2e-15861.70Protein kinase superfamily protein [more]
AT1G56720.33.2e-15861.70Protein kinase superfamily protein [more]
AT2G42960.11.1e-15360.68Protein kinase superfamily protein [more]
AT3G59110.12.0e-15259.02Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 175..448
e-value: 5.8E-38
score: 142.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 176..381
e-value: 3.1E-47
score: 161.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 175..456
score: 39.014801
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 153..250
e-value: 1.5E-33
score: 116.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 251..461
e-value: 1.1E-63
score: 216.5
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 180..372
e-value: 3.3E-17
score: 59.7
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 179..378
e-value: 3.4E-10
score: 37.2
NoneNo IPR availablePANTHERPTHR47984OS01G0323000 PROTEINcoord: 6..456
NoneNo IPR availablePANTHERPTHR47984:SF14OS01G0323000 PROTEINcoord: 6..456
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 181..449
e-value: 2.71789E-107
score: 317.678
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 297..309
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 181..204
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 155..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0026391.1PI0026391.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity