PI0026037 (gene) Melon (PI 482460) v1

BLAST of PI0026037 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 3.9e-312
Identity = 508/744 (68.28%), Postives = 594/744 (79.84%), Query Frame = 0

Query: 62  ARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATLC 121
           A++R +     +AV E+   S  +RF + DG P+PFG    DDG+NF++YS N+VSAT+C
Sbjct: 44  AKDRRSNEAENIAVVEKPLKS--DRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103

Query: 122 LMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEGCYFDP 181
           L+ LSDL+++   +EI LDP  N+TG VWHVFL+GDF +MLYGY+FDGKFSP+EG Y+D 
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163

Query: 182 SRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSIHKKIS 241
           S ILLDPYAKA+ISR+EFG LGP+DNCWPQMA MVPT  E+   FD +      + +K  
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEE---FDWEGDMHLKLPQKDL 223

Query: 242 YIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNS 301
            IYEMHVRGFTRHESS+ EFPGTY G+ EKLD+LKELG+NCIELMPCHEFNELEY+SYN+
Sbjct: 224 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNT 283

Query: 302 VLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVA--------------------------- 361
           +LGD+++NFWGYST+ +FSPMIRY+SA     A                           
Sbjct: 284 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 343

Query: 362 ---RMGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-YW 421
                G +  P  SFRGVDN+VYYMLAP GEFYNYSGCG         + Q  +  L YW
Sbjct: 344 NHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYW 403

Query: 422 VTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVL 481
           VTEMHVDGFRFDL SIM+R SSLWDA NVYG  +EGD+LTTG+P+  PP+IDMISNDP+L
Sbjct: 404 VTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPIL 463

Query: 482 QGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSP 541
           +GVKLIAEAWDAGGLYQVG FPHWG+WSEWNGK+RDVVRQFIKGTDGFSGAFAECLCGSP
Sbjct: 464 RGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSP 523

Query: 542 NLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGE 601
           NLYQ GGRKPW+SINFICAHDGFTLADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+
Sbjct: 524 NLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGD 583

Query: 602 FVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDK 661
           F SISVK+LRKRQMRNFFV LMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NYFRWDK
Sbjct: 584 FASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDK 643

Query: 662 MEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLV 721
            EE+ SDF RFC ++  FR ECESLGLNDFPT +RLQWHG +P  P+W+E SRFVAF+LV
Sbjct: 644 KEEAHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLV 703

Query: 722 DSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVAIQQYA 769
           DSVK+E+Y+AFN SHL   V+LP RPGYRW+P VDT K SP+D +T D+PER+ A++QY 
Sbjct: 704 DSVKKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYR 763

BLAST of PI0026037 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 3.6e-292
Identity = 475/716 (66.34%), Postives = 548/716 (76.54%), Query Frame = 0

Query: 89  VFDGTPTPFGAAALDDGINFAIYSANAVSATLCLMHLSDLQ--ESDQEITLDPLTNKTGD 148
           V  G P P GA ALD G+NFA+YSA A +A+LCL    DL+  E  +E+ LDPL N+TG+
Sbjct: 96  VLAGMPAPLGATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGN 155

Query: 149 VWHVFLKGDFAEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISREEFGALGPNDNC 208
           VWHVF++G+   MLYGY+FDG F+P  G YFD S +++DPYAKAVISR E+G  GP  +C
Sbjct: 156 VWHVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDC 215

Query: 209 WPQMAGMVPTANEKIKNFDGKEIYLCSIHKKISYIYEMHVRGFTRHESSQSEFPGTYLGL 268
           WPQMAGM+P        FD +        +K   IYEMH+RGFT+H SS  E PGTY+G 
Sbjct: 216 WPQMAGMIPL---PYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNVEHPGTYIGA 275

Query: 269 VEKLDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSA 328
           + KLDYLKELGVNC+ELMPCHEFNELEYFS +S     KMNFWGYST+N+FSPMIRYSS 
Sbjct: 276 ISKLDYLKELGVNCVELMPCHEFNELEYFSCSS-----KMNFWGYSTINFFSPMIRYSSG 335

Query: 329 GMGTVAR------------------------------MGMKTAPTISFRGVDNNVYYMLA 388
           G+    R                               G +  P +SFRG+DN+ YYMLA
Sbjct: 336 GIRNCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLA 395

Query: 389 PMGEFYNYSGCGTHLT----AIIQLYVHSL-YWVTEMHVDGFRFDLASIMTRGSSLWDAV 448
           P GEFYNYSGCG         + +  V  L YWVTEMHVDGFRFDLASIMTRG SLWD V
Sbjct: 396 PKGEFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPV 455

Query: 449 NVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVW 508
           NVYG+P+EGD+ TTG+PL  PPLIDMISNDP+L  VKLIAEAWDAGGLYQVG FPHW +W
Sbjct: 456 NVYGSPVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLYQVGQFPHWKIW 515

Query: 509 SEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLAD 568
           SEWNGKYRD+VRQFIKGTDGF+G FAECLCGSP+LYQ GGRKPW+SINF+CAHDGFTLAD
Sbjct: 516 SEWNGKYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLAD 575

Query: 569 LVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGV 628
           LVTYN K+N +NGEDN DGENHN SWNCG+EGEF  +SVK+LRKRQMRNFFV LMVSQGV
Sbjct: 576 LVTYNKKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGV 635

Query: 629 PMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLMTTFRHECESLGL 688
           PM  MGDEYGHTKGGNNNTYCHD+Y+NYFRWDK EES SD  RFCSLMT FR +CESLGL
Sbjct: 636 PMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEES-SDLQRFCSLMTKFRKQCESLGL 695

Query: 689 NDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKQEVYIAFNASHLPVTVTLPERPG 748
            DFPT +RL WHGH PGKPDW+E SRFVAF+  D  K E+Y+AFNASHLP  V LPERPG
Sbjct: 696 ADFPTAQRLHWHGHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERPG 755

Query: 749 YRWDPLVDTGKNSPFDFLTNDIPERDVAIQQYAHFLDANLYPMLSYSSIILLLSPE 768
           YRW+PLVDTGK +P+DFLT+D+P+R  A+  ++HFL++NLYPMLSYSSIIL L P+
Sbjct: 756 YRWEPLVDTGKPAPYDFLTDDLPDRAHAVHLFSHFLNSNLYPMLSYSSIILELQPD 802

BLAST of PI0026037 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 538.1 bits (1385), Expect = 1.6e-151
Identity = 306/707 (43.28%), Postives = 406/707 (57.43%), Query Frame = 0

Query: 73  VAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATLCL-MHLSDLQES 132
           V   E   ++    F+V  G  +P G + +D GINFA++S NA S TLCL +  S   ++
Sbjct: 69  VVEEEASTMTETKLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVTLCLSLSQSGKDDT 128

Query: 133 DQ----EITLDPLTNKTGDVWHVFLKG-DFAEMLYGYKFDGKFSPQEGCYFDPSRILLDP 192
           D     E+ LDP  NKTGD WH+ ++      +LYGY+ DG    Q+G  FD S +LLDP
Sbjct: 129 DDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDP 188

Query: 193 YAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFD-GKEIYLCSIHKKISYIYEMH 252
           YAK V     FG      +   + A    T + +   FD G +    +I +K   IYEM+
Sbjct: 189 YAKLVKGHSSFG------DSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMN 248

Query: 253 VRGFTRHESSQSE--FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNSVLGD 312
           VR FT  ESS  +    G+YLG +EK+ +L++LG+N +EL+P  EF+ELE     S   D
Sbjct: 249 VRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELE-LQRRSNPRD 308

Query: 313 YKMNFWGYSTVNYFSPMIRYS---------------------SAGMGTVA--------RM 372
           + +N WGYSTVN+F+PM RY+                     SAG+  +           
Sbjct: 309 HMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEA 368

Query: 373 GMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-YWVTEMH 432
             K   T SFRG+DN VYYML P  +  N+SGCG  L      +++L + SL +WVTE H
Sbjct: 369 DDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYH 428

Query: 433 VDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKL 492
           VDGFRFDLAS++ R                    T GSPL  PPLI  I+ D VL   K+
Sbjct: 429 VDGFRFDLASVLCRA-------------------TDGSPLSAPPLIRAIAKDSVLSRCKI 488

Query: 493 IAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQG 552
           IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG  G  G+FA  + GS +LYQ 
Sbjct: 489 IAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQV 548

Query: 553 GGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSIS 612
             RKP++ +NF+ AHDGFTL DLV+YN KHN ANGE  NDG N N+SWNCG EGE     
Sbjct: 549 NQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAH 608

Query: 613 VKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEEST 672
           +K LR RQM+NF + LM+SQG PM+LMGDEYGHT+ GNNN+Y HD  LN F+W +++   
Sbjct: 609 IKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKK 668

Query: 673 SDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEA-SRFVAFTLVDSV- 732
            +  RF S +  FRH    L   +F T   + WH     + +W  + S+F+AFTL D + 
Sbjct: 669 QNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNSESKFLAFTLHDGIG 728

Query: 733 KQEVYIAFNASHLPVTVTLPE-RPGYRWDPLVDTGKNSPFDFLTNDI 734
            +++Y+AFNA    V   +P+  PG +W  + DT   SP DF+   +
Sbjct: 729 GRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGV 744

BLAST of PI0026037 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 3.6e-143
Identity = 304/728 (41.76%), Postives = 406/728 (55.77%), Query Frame = 0

Query: 87  FEVFDGTPTPFGAAALDDGINFAIYSANAVSATLCLMHLSDLQESDQ-----EITLDPLT 146
           F+   G   P G + ++ G+NFA++S +A S  LCL       E ++     E  LD   
Sbjct: 98  FKYSSGKAFPLGVSQVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLDQQK 157

Query: 147 NKTGDVWHVFLKG-DFAEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISREEFGAL 206
           NKTGD+WHV ++G   + +LYGY+  G     +G  FD S +LLDPYAK V  R+ FG  
Sbjct: 158 NKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLVSGRKYFGVA 217

Query: 207 GPNDNCWPQMAGMVPTANEKIKNFD-GKEIYLCSIHKKISYIYEMHVRGFTRHESS--QS 266
               +   Q  G   T +     FD G +  L ++ +    IYEM+VR FT  ESS   S
Sbjct: 218 EEKSS---QHFG---TYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDS 277

Query: 267 EFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNYF 326
              G+YLGL++K+ +L ELGVN +EL+P  E++ELE+  Y +   D+ +N WGYST+N+F
Sbjct: 278 TSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNP-RDHMVNTWGYSTINFF 337

Query: 327 SPMIRYSSAGMGTVA-------------RMGMKTAPTI----------------SFRGVD 386
           +PM RY+SAG G VA             + G++    +                SFRG+D
Sbjct: 338 APMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGID 397

Query: 387 NNVYYM--LAPMGEFYNYSGCGTHLT----AIIQLYVHSL-YWVTEMHVDGFRFDLASIM 446
           N VYYM  L    E  N+SGCG  L      + +L + SL +WV E H+DGFRFDLAS++
Sbjct: 398 NKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVL 457

Query: 447 TRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGGLYQ 506
            RG                     G PL  PPLI  I+ D VL   K+IAE WD GGLY 
Sbjct: 458 CRGPD-------------------GCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYL 517

Query: 507 VGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFI 566
           VG FP+W  W+EWNGKYRD +R+FIKG  G  G FA  + GS +LYQ   RKP++ +NF+
Sbjct: 518 VGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFV 577

Query: 567 CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNF 626
            AHDGFTL DLV+YN KHN ANGE   DG N N SWNCG EGE   ++V  LR RQM+NF
Sbjct: 578 IAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNF 637

Query: 627 FVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLMTT 686
            V LM+SQG PM+LMGDEYGHT+ GNNN+Y HD  +N F+W+++E+      RF S M  
Sbjct: 638 HVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMIK 697

Query: 687 FRHECESLGLNDFPTDERLQWHGHSPGKPDW-TEASRFVAFTLVD-SVKQEVYIAFNASH 746
           FRH    L  + F     + WH     +  W  + S+F+AFT+ D +   ++Y+AFNA  
Sbjct: 698 FRHSNPILRRDRFLNKNDVTWH-----EDCWENQESKFLAFTVHDHNSGGDIYLAFNAHD 757

Query: 747 LPVTVTLPERPGYR-WDPLVDTGKNSPFDFLTNDIPERDVAIQQYAHFLDANLYPMLSYS 767
             V   +P  P ++ W+ +VDT   SP D +   +P                 Y +  YS
Sbjct: 758 YFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVP------------FTGPKYRIAPYS 782

BLAST of PI0026037 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.8e-102
Identity = 240/605 (39.67%), Postives = 311/605 (51.40%), Query Frame = 0

Query: 89  VFDGTPTPFGAAALDDGINFAIYSANAVSATLCLMHLSDLQESDQEITLDPLTNKTGDVW 148
           V+ G   P GA     G NF+++S  A    LCL+      + D   +  PL    G VW
Sbjct: 19  VWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCLI------DEDGVESRIPLDEVDGYVW 78

Query: 149 HVFLKGDFAEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISREEFG---------A 208
           H +L        YG++  G F P  G   DPS++LLDPY K+      FG         A
Sbjct: 79  HAYLPNITPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTFGQALYSYDVNA 138

Query: 209 LGPNDNCWPQMAGMVPTANEKIKN--FDGKEIYLCSIHKKISYIYEMHVRGFTR-HESSQ 268
           + P D+  P +  +  T    + N  FD             + IYE HV+G T+ H S  
Sbjct: 139 VDP-DSTPPMVDSLGHTMTSVVINPFFDWAYDRSPRTPYHETVIYEAHVKGMTQTHPSIP 198

Query: 269 SEFPGTYLGLVEK--LDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKM-NFWGYST 328
            E  GTY GL     +D+L EL V  +ELMP H+      F ++S L D  + N+WGY+T
Sbjct: 199 PELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQ------FLHDSRLLDLGLRNYWGYNT 258

Query: 329 VNYFSPMIRYSSAGMGTVARMGMKT---------------------------APTISFRG 388
             +F+P  +Y+S      A    KT                            PTI+FRG
Sbjct: 259 FGFFAPHHQYASTRQAGSAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGPTINFRG 318

Query: 389 VDNNVYYMLAPMG-EFY-NYSGCGTHLTA----IIQLYVHSL-YWVTEMHVDGFRFDLAS 448
           +DN  YY L      FY +++G G  L A     +QL + SL YWV EMHVDGFRFDLAS
Sbjct: 319 IDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGFRFDLAS 378

Query: 449 IMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAG-G 508
            + R     D ++ +                     D++  DPV+  VKLIAE WD G G
Sbjct: 379 TLARELHDVDRLSAF--------------------FDLVQQDPVVSQVKLIAEPWDVGEG 438

Query: 509 LYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 568
            YQVG FP  G+W+EWNGKYRD VR + +G     G FA  L GS +LY+  GR+P  SI
Sbjct: 439 GYQVGNFP--GLWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASI 498

Query: 569 NFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 628
           NF+ AHDGFTL DLV+YN KHN ANGE+N DGE++N SWNCG EG      +  LR RQM
Sbjct: 499 NFVTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQM 558

Query: 629 RNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSL 644
           RN +  LMVSQG PMI  GDE G T+ GNNN YC D+ L++  W  ++++ +D   F   
Sbjct: 559 RNMWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDWSLVDKN-ADLLAFARK 587

BLAST of PI0026037 vs. ExPASy TrEMBL
Match: A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)

HSP 1 Score: 1454.9 bits (3765), Expect = 0.0e+00
Identity = 709/809 (87.64%), Postives = 727/809 (89.86%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRSAASL SS+SFLCVSNLSFPECRRRNL QNVVRGRRR+DI FDLD NWIH+SLN
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATL 120
            ARNRGAEVGT VAVAEE E+SNLNRFEVFDGTPTPFGA A DDGINFAIYSANAVSATL
Sbjct: 61  RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAVSATL 120

Query: 121 CLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEGCYFD 180
           CLMHLSDLQE+   QEITLDPLTNKTGDVWHVFLKGDF EMLYGYKFDGKFSPQEGCYFD
Sbjct: 121 CLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFD 180

Query: 181 PSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSIHKKI 240
           PSRILLDPYAKAVISR EFGALGPNDNCWPQMAGMVPTANEKIKNFD +        +K 
Sbjct: 181 PSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQHAQKD 240

Query: 241 SYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300
             IYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300

Query: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR------------------------- 360
           SVLGDYKMNFWGYSTVNYFSPMIRYSSAG+G   +                         
Sbjct: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVV 360

Query: 361 -----MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-Y 420
                 G +  PTISFRGVDNNVYYMLAPMGEFYNYSGCG        A+    V  L Y
Sbjct: 361 FNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPV 480
           WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMISNDPV
Sbjct: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMISNDPV 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKP NSINF+CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660

Query: 661 KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL 720
           KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL
Sbjct: 661 KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL 720

Query: 721 VDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVAIQQY 773
           VDSVK+EVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPER++AIQQY
Sbjct: 721 VDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQY 780

BLAST of PI0026037 vs. ExPASy TrEMBL
Match: A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)

HSP 1 Score: 1454.9 bits (3765), Expect = 0.0e+00
Identity = 709/809 (87.64%), Postives = 727/809 (89.86%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRSAASL SS+SFLCVSNLSFPECRRRNL QNVVRGRRR+DI FDLD NWIH+SLN
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATL 120
            ARNRGAEVGT VAVAEE E+SNLNRFEVFDGTPTPFGA A DDGINFAIYSANAVSATL
Sbjct: 61  RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAVSATL 120

Query: 121 CLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEGCYFD 180
           CLMHLSDLQE+   QEITLDPLTNKTGDVWHVFLKGDF EMLYGYKFDGKFSPQEGCYFD
Sbjct: 121 CLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFD 180

Query: 181 PSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSIHKKI 240
           PSRILLDPYAKAVISR EFGALGPNDNCWPQMAGMVPTANEKIKNFD +        +K 
Sbjct: 181 PSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQHAQKD 240

Query: 241 SYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300
             IYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300

Query: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR------------------------- 360
           SVLGDYKMNFWGYSTVNYFSPMIRYSSAG+G   +                         
Sbjct: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVV 360

Query: 361 -----MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-Y 420
                 G +  PTISFRGVDNNVYYMLAPMGEFYNYSGCG        A+    V  L Y
Sbjct: 361 FNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPV 480
           WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMISNDPV
Sbjct: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMISNDPV 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKP NSINF+CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660

Query: 661 KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL 720
           KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL
Sbjct: 661 KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL 720

Query: 721 VDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVAIQQY 773
           VDSVK+EVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPER++AIQQY
Sbjct: 721 VDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQY 780

BLAST of PI0026037 vs. ExPASy TrEMBL
Match: A0A6J1EW54 (isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 SV=1)

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 650/813 (79.95%), Postives = 693/813 (85.24%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRS ASLSSS SFL  SN SFPE RRR+   NV+  RRRSDI  DL V+WIH+ +N
Sbjct: 1   MELVTRS-ASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRIN 60

Query: 61  ----GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAV 120
                A +RGAEV T V VAEEVE+SN NRFEVF+GTPTPFGA A D GINFAIYS NAV
Sbjct: 61  VSETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAV 120

Query: 121 SATLCLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEG 180
           SATLCL++L DLQ+    QEITLDPL N+TGDVWHVFLKGDF +MLYGYKF GKFSPQEG
Sbjct: 121 SATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSI 240
           CYFDPSRILLDPYAKAVISREEFGALGP+DNCWPQMAGMVPT +++   FD +       
Sbjct: 181 CYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQH 240

Query: 241 HKKISYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
            +K   IYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 PQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR--------------------- 360
           +SYNSVLGDYK+NFWGYSTV + SPMIRYSSAG+    +                     
Sbjct: 301 YSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVI 360

Query: 361 ---------MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVH 420
                     G +  P  SFRGVDNNVYYMLAPMGEFYNYSGCG        A+ +  V 
Sbjct: 361 MDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 420

Query: 421 SL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
            L YWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540
           NDP+LQGVKLIAEAWDAGGLYQVGTFPHW VWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NY
Sbjct: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINY 660

Query: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720
           FRWDKMEES+SDFHRFC LMTTFR+ECESLGLNDFPT +RLQWHGHSPG PDW+EASRFV
Sbjct: 661 FRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFV 720

Query: 721 AFTLVDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVA 773
           AFTLVDSVK+E+YIAFNASHLPVTVTLPERPGY+W+PLVDTGK SPFDFLT+D+ ERDVA
Sbjct: 721 AFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVA 780

BLAST of PI0026037 vs. ExPASy TrEMBL
Match: A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)

HSP 1 Score: 1315.8 bits (3404), Expect = 0.0e+00
Identity = 648/813 (79.70%), Postives = 692/813 (85.12%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRS ASLSSS SFL  SN SFPE RRR+  +NV+  RRRSDI  DLDV+WIH+ +N
Sbjct: 1   MELVTRS-ASLSSSVSFLRTSNSSFPEFRRRSSKRNVIPIRRRSDIHLDLDVDWIHSRIN 60

Query: 61  ----GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAV 120
                A +RGAEV T V +AEEVE+SN NRFEVF+GTPTPFGA A D GINFAIYS NAV
Sbjct: 61  VSETRASSRGAEVRTSVTMAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAV 120

Query: 121 SATLCLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEG 180
           SATLCL++L DLQ+    QEITLDPLTN+TGDVWHVFLKGDF +MLYGYKF GKFSPQEG
Sbjct: 121 SATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSI 240
           CYFDPSRILLDPYAKAVISREEFGALGP+DNCWPQMAGMVPT +++   FD +       
Sbjct: 181 CYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQH 240

Query: 241 HKKISYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
            +K   IYEMHVRGFTRHESSQS  PGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 PQKDLIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR--------------------- 360
           +SYNSVLGDYK+NFWGYSTV + SPMIRYSSAG+    +                     
Sbjct: 301 YSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVI 360

Query: 361 ---------MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVH 420
                     G +  P  SFRGVDNNVYYMLAPMGEFYNYSGCG        A+ +  V 
Sbjct: 361 MDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 420

Query: 421 SL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
            L YWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540
           NDP+LQGVKLIAEAWDAGGLYQVGTFPHW VWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NY
Sbjct: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINY 660

Query: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720
           F WDKMEES+SDFHRFC LMTTFR+ECESLGLNDFPT +RLQWHGHSPG PDW+EASRFV
Sbjct: 661 FLWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFV 720

Query: 721 AFTLVDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVA 773
           AFTLVDSVK+E+YIAFNASHLPVTVTLPER GYRW+PLVDTGK SPFDFLT+D+ ERDVA
Sbjct: 721 AFTLVDSVKREIYIAFNASHLPVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVA 780

BLAST of PI0026037 vs. ExPASy TrEMBL
Match: A0A6J1BZK5 (isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 SV=1)

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 642/812 (79.06%), Postives = 690/812 (84.98%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHA--- 60
           ME+VTRS  SL  S+ FLC     FPECRRR+   N VR RRRSDI  DLD NWI++   
Sbjct: 1   MELVTRS-TSLCWSACFLCAPKSRFPECRRRSSKNNAVRSRRRSDIHMDLDANWINSRMK 60

Query: 61  -SLNGARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAV 120
            SL  A NRGAEVGT VA+AEEVEVSNL+RFEVF+G P P GA A D G+NFA+YS NAV
Sbjct: 61  MSLTRASNRGAEVGTSVAMAEEVEVSNLHRFEVFEGDPAPLGATARDGGMNFAVYSGNAV 120

Query: 121 SATLCLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEG 180
           SATLCLM+L DLQE+   +E+TLDPLTNKTGDVWHVFLKG F EMLYGYKFDG+FSPQ+G
Sbjct: 121 SATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVFLKGGFTEMLYGYKFDGEFSPQDG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSI 240
           CYFDPSRILLDPYAKAVISR EFGA GP++NCWPQMAGMVPT    I  FD +       
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMAGMVPTV---IDEFDWEGDLPLQY 240

Query: 241 HKKISYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
            +K   IYEMHVRGFTRHESS SEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 PQKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR--------------------- 360
           FSYNSVLGDYKMNFWGYSTV +FSPMIRYSSAG+    +                     
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 ---------MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVH 420
                     G +  P  SFRGVDN+VYYMLAPMGEFYNYSGCG        A+ +  V 
Sbjct: 361 MDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 420

Query: 421 SL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
            L YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540
           NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNG+YRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYN+KHNLANGEDNNDGE+HNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660
           GQEGEFVS+SVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NY
Sbjct: 601 GQEGEFVSMSVKKLRKRQMRNFFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINY 660

Query: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720
           FRWDK+EES+SDFHRFCSL+TTFRHECESLGLNDFPT ERLQWHG+SPGKPDW+EASRFV
Sbjct: 661 FRWDKLEESSSDFHRFCSLVTTFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFV 720

Query: 721 AFTLVDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVA 772
           AFTLVDSVK E+YIAFNA+HLPVTVTLPERPGYRW+PLVDTGK SPFDFL++D+PERDVA
Sbjct: 721 AFTLVDSVKGEIYIAFNANHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVA 780

BLAST of PI0026037 vs. NCBI nr
Match: XP_008453530.1 (PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1454.9 bits (3765), Expect = 0.0e+00
Identity = 709/809 (87.64%), Postives = 727/809 (89.86%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRSAASL SS+SFLCVSNLSFPECRRRNL QNVVRGRRR+DI FDLD NWIH+SLN
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATL 120
            ARNRGAEVGT VAVAEE E+SNLNRFEVFDGTPTPFGA A DDGINFAIYSANAVSATL
Sbjct: 61  RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAVSATL 120

Query: 121 CLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEGCYFD 180
           CLMHLSDLQE+   QEITLDPLTNKTGDVWHVFLKGDF EMLYGYKFDGKFSPQEGCYFD
Sbjct: 121 CLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFD 180

Query: 181 PSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSIHKKI 240
           PSRILLDPYAKAVISR EFGALGPNDNCWPQMAGMVPTANEKIKNFD +        +K 
Sbjct: 181 PSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQHAQKD 240

Query: 241 SYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300
             IYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300

Query: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR------------------------- 360
           SVLGDYKMNFWGYSTVNYFSPMIRYSSAG+G   +                         
Sbjct: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVV 360

Query: 361 -----MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-Y 420
                 G +  PTISFRGVDNNVYYMLAPMGEFYNYSGCG        A+    V  L Y
Sbjct: 361 FNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPV 480
           WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMISNDPV
Sbjct: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMISNDPV 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKP NSINF+CAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPCNSINFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660

Query: 661 KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL 720
           KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL
Sbjct: 661 KMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTL 720

Query: 721 VDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVAIQQY 773
           VDSVK+EVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPER++AIQQY
Sbjct: 721 VDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQY 780

BLAST of PI0026037 vs. NCBI nr
Match: XP_004149676.1 (isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein Csa_019536 [Cucumis sativus])

HSP 1 Score: 1439.9 bits (3726), Expect = 0.0e+00
Identity = 704/810 (86.91%), Postives = 725/810 (89.51%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VT SAASL SS+SFLCVSNLSFPECRRRN  QNVVRGRRRSDI FDLD NWIH+SLN
Sbjct: 1   MELVTHSAASLFSSASFLCVSNLSFPECRRRNSKQNVVRGRRRSDICFDLDANWIHSSLN 60

Query: 61  GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATL 120
            ARNRGAEV T VAVAEEVEVSNLNRFEVFDGTPTPFGA A +DGINFAIYSANAVSATL
Sbjct: 61  RARNRGAEVRTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSATL 120

Query: 121 CLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEGCYFD 180
           CL+HLSDLQE+   QEITLDPLTNKTGDVWHVFLKGDF EMLYGYKFDGKFSPQEGCYFD
Sbjct: 121 CLIHLSDLQENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFD 180

Query: 181 PSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSIHKKI 240
            SRILLDPYAKAVISR EFGALGPNDNCWPQMAGMVPTANEKIK+FD +        +K 
Sbjct: 181 SSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQHAQKD 240

Query: 241 SYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYN 300
             IYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELG+NCIELMPCHEFNELEYFSYN
Sbjct: 241 LIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEYFSYN 300

Query: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR------------------------- 360
           SVLGDYKMNFWGYSTVNYFSPMIRYSSAG+G   +                         
Sbjct: 301 SVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVV 360

Query: 361 -----MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-Y 420
                 G +  PTISFRGVDNNVYYMLAPMGEFYNYSGCG        A+    V  L Y
Sbjct: 361 FNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLRY 420

Query: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPV 480
           WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYG+PIEGD+LTTGSPLGNPPLIDMISNDPV
Sbjct: 421 WVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGSPIEGDILTTGSPLGNPPLIDMISNDPV 480

Query: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGS 540
           LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDV+RQFIKGTDGFSGAFAECLCGS
Sbjct: 481 LQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVIRQFIKGTDGFSGAFAECLCGS 540

Query: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEG 600
           PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSK NLANGE+NNDGENHNNSWNCGQEG
Sbjct: 541 PNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKQNLANGEENNDGENHNNSWNCGQEG 600

Query: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660
           EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD
Sbjct: 601 EFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWD 660

Query: 661 KMEESTS-DFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFT 720
           KMEESTS DFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFT
Sbjct: 661 KMEESTSFDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFT 720

Query: 721 LVDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVAIQQ 773
           LVDSVK+EVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIP+RDVAIQQ
Sbjct: 721 LVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPKRDVAIQQ 780

BLAST of PI0026037 vs. NCBI nr
Match: XP_038879251.1 (isoamylase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 687/813 (84.50%), Postives = 717/813 (88.19%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRS ASLSSS+SFLC S L+FPECRRRN  QNVVR RRRSDIRFDLDVNW H+ +N
Sbjct: 1   MELVTRS-ASLSSSASFLCASKLNFPECRRRNSKQNVVRDRRRSDIRFDLDVNWFHSRMN 60

Query: 61  G----ARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAV 120
                A NRGA+VGT +A+AEEVEVS  NRFEVFDGTPTPFGA   D G+NFAIYSANAV
Sbjct: 61  ASVTRAWNRGAKVGTSIAMAEEVEVSISNRFEVFDGTPTPFGATTRDGGVNFAIYSANAV 120

Query: 121 SATLCLMHLSDLQESD--QEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEG 180
           SATLCL++LSDL E+   QEITLDPLTNKTGDVWHVFLKGDF EMLYGYKF+GKFSPQEG
Sbjct: 121 SATLCLINLSDLHENSVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFEGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSI 240
           CYFDPSRILLDPYAKAVISR EFGALGPNDNCWPQMAGMVPT NE+IKNFD +       
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTINEEIKNFDWEGDLPLQH 240

Query: 241 HKKISYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
            +K   IYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR--------------------- 360
           FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSA +G   +                     
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSASIGNCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 ---------MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVH 420
                     G +  PTISFRGVDNNVYYMLAPMGEFYNYSGCG        A+    V 
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420

Query: 421 SL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
            L YWVTEMHVDGFRFDLASIMTRGSSLWDAVNV+GNPIEGDVLTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVFGNPIEGDVLTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540
           NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNG+YRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVP+ILMGDEYGHTKGGNNNTYCHDNYLNY
Sbjct: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPLILMGDEYGHTKGGNNNTYCHDNYLNY 660

Query: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720
           FRWDKMEES+SDFHRFCSLMTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV
Sbjct: 661 FRWDKMEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720

Query: 721 AFTLVDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVA 773
           AFTLVDSVK+EVYIAFNASHLPVTVTLPERPGYRWDPLVDTGK SPFDFLT+D+PERDVA
Sbjct: 721 AFTLVDSVKREVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVA 780

BLAST of PI0026037 vs. NCBI nr
Match: KAG7021674.1 (Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1323.1 bits (3423), Expect = 0.0e+00
Identity = 650/813 (79.95%), Postives = 694/813 (85.36%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRS ASLSSS SFL  SN SFPE RRR+   NV+  RRRSDI  DLDV+WIH+ +N
Sbjct: 1   MELVTRS-ASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLDVDWIHSRIN 60

Query: 61  ----GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAV 120
                A +RGAEV T V VAEEVE+SN NRFEVF+GTPTPFGA A D GINFAIYS NAV
Sbjct: 61  VSETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAV 120

Query: 121 SATLCLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEG 180
           SATLCL++L DLQ+    QEITLDPL N+TGDVWHVFLKGDF +MLYGYKF GKFSPQEG
Sbjct: 121 SATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSI 240
           CYFDPSRILLDPYAKAVISREEFGALGP+DNCWPQMAGMVPT +++   FD +       
Sbjct: 181 CYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQH 240

Query: 241 HKKISYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
            +K   IYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 PQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR--------------------- 360
           +SYNSVLGDYK+NFWGYSTV + SPMIRYSSAG+    +                     
Sbjct: 301 YSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKFLIKEAHKRGIEVI 360

Query: 361 ---------MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVH 420
                     G +  P  SFRGVDNNVYYMLAPMGEFYNYSGCG        A+ +  V 
Sbjct: 361 MDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 420

Query: 421 SL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
            L YWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540
           NDP+LQGVKLIAEAWDAGGLYQVGTFPHW VWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NY
Sbjct: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINY 660

Query: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720
           FRWDKMEES+SDFHRFC LMTTFR+ECESLGLNDFPT +RLQWHGHSPG PDW+EASRFV
Sbjct: 661 FRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFV 720

Query: 721 AFTLVDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVA 773
           AFTLVDSVK+E+YIAFNASHLPVTVTLPERPGY+W+PLVDTGK SPFDFLT+D+ +RDVA
Sbjct: 721 AFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEKRDVA 780

BLAST of PI0026037 vs. NCBI nr
Match: XP_022932124.1 (isoamylase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 650/813 (79.95%), Postives = 693/813 (85.24%), Query Frame = 0

Query: 1   MEMVTRSAASLSSSSSFLCVSNLSFPECRRRNLNQNVVRGRRRSDIRFDLDVNWIHASLN 60
           ME+VTRS ASLSSS SFL  SN SFPE RRR+   NV+  RRRSDI  DL V+WIH+ +N
Sbjct: 1   MELVTRS-ASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRIN 60

Query: 61  ----GARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAV 120
                A +RGAEV T V VAEEVE+SN NRFEVF+GTPTPFGA A D GINFAIYS NAV
Sbjct: 61  VSETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAV 120

Query: 121 SATLCLMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEG 180
           SATLCL++L DLQ+    QEITLDPL N+TGDVWHVFLKGDF +MLYGYKF GKFSPQEG
Sbjct: 121 SATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSI 240
           CYFDPSRILLDPYAKAVISREEFGALGP+DNCWPQMAGMVPT +++   FD +       
Sbjct: 181 CYFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQH 240

Query: 241 HKKISYIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
            +K   IYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 PQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVAR--------------------- 360
           +SYNSVLGDYK+NFWGYSTV + SPMIRYSSAG+    +                     
Sbjct: 301 YSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVI 360

Query: 361 ---------MGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVH 420
                     G +  P  SFRGVDNNVYYMLAPMGEFYNYSGCG        A+ +  V 
Sbjct: 361 MDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVD 420

Query: 421 SL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
            L YWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAEC 540
           NDP+LQGVKLIAEAWDAGGLYQVGTFPHW VWSEWNGKYRD+VRQFIKGTDGFSGAFAEC
Sbjct: 481 NDPILQGVKLIAEAWDAGGLYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAEC 540

Query: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600
           LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC
Sbjct: 541 LCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNC 600

Query: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNY 660
           GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NY
Sbjct: 601 GQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINY 660

Query: 661 FRWDKMEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFV 720
           FRWDKMEES+SDFHRFC LMTTFR+ECESLGLNDFPT +RLQWHGHSPG PDW+EASRFV
Sbjct: 661 FRWDKMEESSSDFHRFCRLMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFV 720

Query: 721 AFTLVDSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVA 773
           AFTLVDSVK+E+YIAFNASHLPVTVTLPERPGY+W+PLVDTGK SPFDFLT+D+ ERDVA
Sbjct: 721 AFTLVDSVKREIYIAFNASHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVA 780

BLAST of PI0026037 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 1071.6 bits (2770), Expect = 2.8e-313
Identity = 508/744 (68.28%), Postives = 594/744 (79.84%), Query Frame = 0

Query: 62  ARNRGAEVGTLVAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATLC 121
           A++R +     +AV E+   S  +RF + DG P+PFG    DDG+NF++YS N+VSAT+C
Sbjct: 44  AKDRRSNEAENIAVVEKPLKS--DRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103

Query: 122 LMHLSDLQES--DQEITLDPLTNKTGDVWHVFLKGDFAEMLYGYKFDGKFSPQEGCYFDP 181
           L+ LSDL+++   +EI LDP  N+TG VWHVFL+GDF +MLYGY+FDGKFSP+EG Y+D 
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163

Query: 182 SRILLDPYAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFDGKEIYLCSIHKKIS 241
           S ILLDPYAKA+ISR+EFG LGP+DNCWPQMA MVPT  E+   FD +      + +K  
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEE---FDWEGDMHLKLPQKDL 223

Query: 242 YIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNS 301
            IYEMHVRGFTRHESS+ EFPGTY G+ EKLD+LKELG+NCIELMPCHEFNELEY+SYN+
Sbjct: 224 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNT 283

Query: 302 VLGDYKMNFWGYSTVNYFSPMIRYSSAGMGTVA--------------------------- 361
           +LGD+++NFWGYST+ +FSPMIRY+SA     A                           
Sbjct: 284 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 343

Query: 362 ---RMGMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-YW 421
                G +  P  SFRGVDN+VYYMLAP GEFYNYSGCG         + Q  +  L YW
Sbjct: 344 NHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYW 403

Query: 422 VTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVL 481
           VTEMHVDGFRFDL SIM+R SSLWDA NVYG  +EGD+LTTG+P+  PP+IDMISNDP+L
Sbjct: 404 VTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPIL 463

Query: 482 QGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSP 541
           +GVKLIAEAWDAGGLYQVG FPHWG+WSEWNGK+RDVVRQFIKGTDGFSGAFAECLCGSP
Sbjct: 464 RGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSP 523

Query: 542 NLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGE 601
           NLYQ GGRKPW+SINFICAHDGFTLADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+
Sbjct: 524 NLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGD 583

Query: 602 FVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDK 661
           F SISVK+LRKRQMRNFFV LMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NYFRWDK
Sbjct: 584 FASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDK 643

Query: 662 MEESTSDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLV 721
            EE+ SDF RFC ++  FR ECESLGLNDFPT +RLQWHG +P  P+W+E SRFVAF+LV
Sbjct: 644 KEEAHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLV 703

Query: 722 DSVKQEVYIAFNASHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERDVAIQQYA 769
           DSVK+E+Y+AFN SHL   V+LP RPGYRW+P VDT K SP+D +T D+PER+ A++QY 
Sbjct: 704 DSVKKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYR 763

BLAST of PI0026037 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 538.1 bits (1385), Expect = 1.2e-152
Identity = 306/707 (43.28%), Postives = 406/707 (57.43%), Query Frame = 0

Query: 73  VAVAEEVEVSNLNRFEVFDGTPTPFGAAALDDGINFAIYSANAVSATLCL-MHLSDLQES 132
           V   E   ++    F+V  G  +P G + +D GINFA++S NA S TLCL +  S   ++
Sbjct: 69  VVEEEASTMTETKLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVTLCLSLSQSGKDDT 128

Query: 133 DQ----EITLDPLTNKTGDVWHVFLKG-DFAEMLYGYKFDGKFSPQEGCYFDPSRILLDP 192
           D     E+ LDP  NKTGD WH+ ++      +LYGY+ DG    Q+G  FD S +LLDP
Sbjct: 129 DDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDP 188

Query: 193 YAKAVISREEFGALGPNDNCWPQMAGMVPTANEKIKNFD-GKEIYLCSIHKKISYIYEMH 252
           YAK V     FG      +   + A    T + +   FD G +    +I +K   IYEM+
Sbjct: 189 YAKLVKGHSSFG------DSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMN 248

Query: 253 VRGFTRHESSQSE--FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNSVLGD 312
           VR FT  ESS  +    G+YLG +EK+ +L++LG+N +EL+P  EF+ELE     S   D
Sbjct: 249 VRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELE-LQRRSNPRD 308

Query: 313 YKMNFWGYSTVNYFSPMIRYS---------------------SAGMGTVA--------RM 372
           + +N WGYSTVN+F+PM RY+                     SAG+  +           
Sbjct: 309 HMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEA 368

Query: 373 GMKTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLT----AIIQLYVHSL-YWVTEMH 432
             K   T SFRG+DN VYYML P  +  N+SGCG  L      +++L + SL +WVTE H
Sbjct: 369 DDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYH 428

Query: 433 VDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKL 492
           VDGFRFDLAS++ R                    T GSPL  PPLI  I+ D VL   K+
Sbjct: 429 VDGFRFDLASVLCRA-------------------TDGSPLSAPPLIRAIAKDSVLSRCKI 488

Query: 493 IAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQG 552
           IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG  G  G+FA  + GS +LYQ 
Sbjct: 489 IAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQV 548

Query: 553 GGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSIS 612
             RKP++ +NF+ AHDGFTL DLV+YN KHN ANGE  NDG N N+SWNCG EGE     
Sbjct: 549 NQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAH 608

Query: 613 VKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEEST 672
           +K LR RQM+NF + LM+SQG PM+LMGDEYGHT+ GNNN+Y HD  LN F+W +++   
Sbjct: 609 IKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKK 668

Query: 673 SDFHRFCSLMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEA-SRFVAFTLVDSV- 732
            +  RF S +  FRH    L   +F T   + WH     + +W  + S+F+AFTL D + 
Sbjct: 669 QNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNSESKFLAFTLHDGIG 728

Query: 733 KQEVYIAFNASHLPVTVTLPE-RPGYRWDPLVDTGKNSPFDFLTNDI 734
            +++Y+AFNA    V   +P+  PG +W  + DT   SP DF+   +
Sbjct: 729 GRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFVREGV 744

BLAST of PI0026037 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 268.5 bits (685), Expect = 1.7e-71
Identity = 206/668 (30.84%), Postives = 315/668 (47.16%), Query Frame = 0

Query: 92  GTPTPFGAAA--LDDGINFAIYSANAVSATLCLMHLSDLQESDQEITLDPLTNKTGDVWH 151
           G P P G ++   DD  NF+ +S ++ +  LCL   S   +   E+ LDP  N+TGDVWH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 152 VFLKGDFAEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAV---ISREEFGALGPNDNC 211
             +   +  + YGY+       +E    +   I+LDPYA  V   +S++  G+L      
Sbjct: 290 ASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSL------ 349

Query: 212 WPQMAGMVPTANEKIKNFDGKEIYLCSIHKKISYIYEMHVRGFTRHESSQ--SEFPGTYL 271
                        K  +FD  E    +I  +   +Y ++V+GFT+H SS+  S   GT+ 
Sbjct: 350 ------------SKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFS 409

Query: 272 GLVEKLDYLKELGVNCIELMPCHEFNELE--YFSYN--SVLGDYKMNFWGYSTVNYFSPM 331
           G+ EK+ +LK LG N + L P   F+E +  YF ++  S +  Y  +    S VN    M
Sbjct: 410 GVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVM 469

Query: 332 I-RYSSAGMGTVARMGM-KTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLTAIIQLY 391
           + +  S G+  +  +    TA + + RG+D++ YY      +  + S    +   + QL 
Sbjct: 470 VKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLV 529

Query: 392 VHSL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDM 451
           + SL YWVTE HVDGF F  AS + RG                     G  L  PPL++ 
Sbjct: 530 LESLRYWVTEFHVDGFCFINASSLLRG-------------------VHGEQLSRPPLVEA 589

Query: 452 ISNDPVLQGVKLIAEAWDAGGLY-QVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAF 511
           I+ DP+L   KLIA+ WD   +  +   FPHW  W+E N +Y   VR F++G  G     
Sbjct: 590 IAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDL 649

Query: 512 AECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNS 571
           A  +CGS +++   GR P  S N+I  + G +L D+V++ S   LA+            S
Sbjct: 650 ATRICGSGDVFT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS----------ELS 709

Query: 572 WNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNY 631
           WNCG+EG     +V + R +Q+RNF     +S GVP++ MGDE G +  G+         
Sbjct: 710 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESR--- 769

Query: 632 LNYFRWDKMEES-TSDFHRFCSLMTTFR-HECESLGLNDFPTDERLQWHGHSPGKPDWTE 691
              F W+ +  +  +   +F S MT+ R    +     DF   E + W+ +    P W +
Sbjct: 770 -KPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 829

Query: 692 -ASRFVAFTLVDSVKQE---------------VYIAFNASHLPVTVTLPERP-GYRWDPL 726
            AS+F+A  +    ++E               ++I FNAS  P +V LP  P G +W  L
Sbjct: 830 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 843

BLAST of PI0026037 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 268.5 bits (685), Expect = 1.7e-71
Identity = 206/668 (30.84%), Postives = 315/668 (47.16%), Query Frame = 0

Query: 92  GTPTPFGAAA--LDDGINFAIYSANAVSATLCLMHLSDLQESDQEITLDPLTNKTGDVWH 151
           G P P G ++   DD  NF+ +S ++ +  LCL   S   +   E+ LDP  N+TGDVWH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 152 VFLKGDFAEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAV---ISREEFGALGPNDNC 211
             +   +  + YGY+       +E    +   I+LDPYA  V   +S++  G+L      
Sbjct: 290 ASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSL------ 349

Query: 212 WPQMAGMVPTANEKIKNFDGKEIYLCSIHKKISYIYEMHVRGFTRHESSQ--SEFPGTYL 271
                        K  +FD  E    +I  +   +Y ++V+GFT+H SS+  S   GT+ 
Sbjct: 350 ------------SKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFS 409

Query: 272 GLVEKLDYLKELGVNCIELMPCHEFNELE--YFSYN--SVLGDYKMNFWGYSTVNYFSPM 331
           G+ EK+ +LK LG N + L P   F+E +  YF ++  S +  Y  +    S VN    M
Sbjct: 410 GVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVM 469

Query: 332 I-RYSSAGMGTVARMGM-KTAPTISFRGVDNNVYYMLAPMGEFYNYSGCGTHLTAIIQLY 391
           + +  S G+  +  +    TA + + RG+D++ YY      +  + S    +   + QL 
Sbjct: 470 VKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLV 529

Query: 392 VHSL-YWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDM 451
           + SL YWVTE HVDGF F  AS + RG                     G  L  PPL++ 
Sbjct: 530 LESLRYWVTEFHVDGFCFINASSLLRG-------------------VHGEQLSRPPLVEA 589

Query: 452 ISNDPVLQGVKLIAEAWDAGGLY-QVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAF 511
           I+ DP+L   KLIA+ WD   +  +   FPHW  W+E N +Y   VR F++G  G     
Sbjct: 590 IAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDL 649

Query: 512 AECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNS 571
           A  +CGS +++   GR P  S N+I  + G +L D+V++ S   LA+            S
Sbjct: 650 ATRICGSGDVFT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS----------ELS 709

Query: 572 WNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNY 631
           WNCG+EG     +V + R +Q+RNF     +S GVP++ MGDE G +  G+         
Sbjct: 710 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESR--- 769

Query: 632 LNYFRWDKMEES-TSDFHRFCSLMTTFR-HECESLGLNDFPTDERLQWHGHSPGKPDWTE 691
              F W+ +  +  +   +F S MT+ R    +     DF   E + W+ +    P W +
Sbjct: 770 -KPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 829

Query: 692 -ASRFVAFTLVDSVKQE---------------VYIAFNASHLPVTVTLPERP-GYRWDPL 726
            AS+F+A  +    ++E               ++I FNAS  P +V LP  P G +W  L
Sbjct: 830 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 843