PI0025986 (gene) Melon (PI 482460) v1

Overview
NamePI0025986
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptioncytochrome P450 714C2-like
Locationchr05: 16249794 .. 16253259 (-)
RNA-Seq ExpressionPI0025986
SyntenyPI0025986
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCAATGATGGTACTCTCTGTAATAGCCGTTATTTTCTTCTTCATTTTTCTTCACCTCTTTGAATCGCTGTTCTTGAAACCGGAAAGACTACGATCGAAGCTCCGTCGACAAGGAATCAAGGGTCCTTCCCCTTCTTTTCTCCTCGGAAACATACCGGAAATTAAGAACATTAGAAGCTTAAAATCATTTGGTGAAGAAGACGATTCGATTGCTCATGGTTGGTCTTCCAATCTGCTTCCCCATTTGGAGCACTGGCGGAATCGATACGGTAATTTCTTTATCTTTGTGTATATTAGACGATTCTTTATAGAACTAATTGTAGTTATTTAGCTAAACTTTGGTCTGTACATATATACACACTTCTCAGTTACTACGATACGCAGACCGTATAAAGGGGAGAGGCTGCATGTGATTAGGTGTTTCTTTTAAAACTCATGTTTATAAAATTAGTTTGGAGTTTTCAAAATGTATTTTTCTTTTTTAGTTTATAAAAATAGGGTTTACAGGTCTCATGATTTTTAGAATTACAAAAAATAGTTTGGGAGAAGGGGAATTGTAAAAAATGGTAAATTTGACAAACTATTTACACTTTATGATGTAATGATCTTGTGTACTTTAGTGATTTTTTTGTGATAAAATGTAAATAGTTTGTATTTTTTATTATTCTCGAAAAAACTCCATTTTTAAATTAATTTTTTATTTAATGTTTTATATAATTATTTACAATAAAGTGTATATGGTTATTTGAGAACTTTTTAAAACTATTTTAAAAGATAAGGAACTAAAAAGATATAATTGATTTGGAAATTAAATAAGTTTATTTTTAAAACTCGATGTCTAAAAATGTATATTGTTAAAATTCATGGTCCAAACACATAATCTTCAAAACTTTGAAACTAGAAAGGTAGTTTTTTTTTTTTTTTTTTTTTAAATTTAAAATAAATAAAAAAATCAGGGTAAACAAGTAGAAACCAAAATGGGGATAAAAATGGAGGAGAATAAAAAACCCTTCTTCTGGCATGTGAAATTTTTTTTGGAATTTGGCAAAACATAAGAAAGATAGTTTTAAATAGGTTTAATTTTGAAAAAAAAAAAACAATTGAAACATTTTTTTTTTTTAAATAGTAAGATTTTTCCAAAATTATTGTCTACAATTGCAAAAGCTTTGCCACATGGAGGTTAATCCCTAAAACAAAAAATAGGTTAAGAAACCCATTTTTTTAAAAATGGTTTATAAGGGTGTTCAAAATGAAATATTAGTGATTGGTGGATTTTAGGAAGAAACTTCGTGTATTCGAGCGGGACGGTTCAAATATTGTGCATAACGGACGTTGAGATGGTGAAGGAAATTGGTATGTCAACGACGTTAAGTTTGGGAAAGCCTGCCCACCTTTCCAAGGATCGTGGGCCGTTATTGGGCCTCGGCATTTTGGCATCAAATGGCCCACTTTGGGTCCACCAAAGGAAGACCTCCTCAACTCTATCTTGATAAAGTCAAGGTAAAAAAGTATATCATTTCTTCGTACTTCACTTTTTTTTTTCTATTAAGTATGTGATTCATACCTCCTTCAATTTAAAAACGATCTAACAACTTCTTCAAACATCATTGAACTTATAATTCTATATAGCTTAATCCATGAACTTCCATAGTTTTGTCTCTTTGGTTTGTAAATTTTCAATTTTGTATGTCGTAGATTTGTGAATTTTAAAAAATTTAAATGTTCACATATTAAAGACTCGTTTGAATTACTAAAAGAAGTATTTTTTTTTTCCTAAAATAAATTCATTTTTATTTAAATCTTATTTTGATATGAAACTGTTTAGAATAAATTATGAAAATGTTTTAAAAACTATTTTGAGTGGTTGCCTACCAATTGAATATTATCAAAATTAAACACTTGAAAAGTTAGACCAAACATATCTTAAATTTGTAATTTTGAAAGCTTTTGGAACTAATTAGACATTCATAGATTAAATCTATACTTCAAGTCAACCTATTTGACATGAAACTAACTTTGGGTAAAATAAGTCCATAAAATTTATCCTCAGTGTAGTTTAACAGTTCTAGAAAGACAATCCATTGATTTATGGGGCTAATTATTCCGCATATAAAATAAAATATGAACTGAGATATCTTTGAATCTATAGGACATGACAAAGCTTATGGTGGAATCTGTAAACTCTTTGGTAAAATCATGGGAAACCATAATTGACAATGGTAGAGGAGAATCAGAAATCAACGTGGATGGATATTTTAGAGTCATGTCTGCTGATGTTATCTCGAAAGCCTGTTTCGGAAGTAATTTTTATGAGGGGAAAGAGATATTTCAAAAACTCAAAGCTCTTCAAATTATCATGTCCAAAGCAAGCATTGGCATTCCTGGGTTCAGGTATATATGTGGGCAATTTGATTCTCTGATTTCAAGAAATATAGTACATGCATGTTAATTACTATTGTTGTTGAGGTATGCGTACTTTGACACCGAAACCCGTTATCAATTTGATTAAATATTGATTTGATTTATATTGTGTGCTTTAGGTAACAGATCTACAATTTTATGTGCTAGGTATCTCCCCACCAAAAATAATAGAGAAATATGGAAGCTTGAGAAGGAAGTAGAATCAATGGTTTTAGATGTGGTAAACAAACGAATCAAGCAATGCTCAAATGAAAAAGACTTGTTGCAATAATTATTGATGGTGCGAAATGTCTTAATGAAGAGGGTAACTCGTTGAAGATATCTAGCGACAAGTTCATTGTTGATAACTGCAAAAACATATACTTTGCTGGCCACGAGACAACATCAATAATGGCATCATGGTGCTTGTTGTTATTAGCAATACACCAAGACTGGCAAGCTCGTGTTCGTTCCGAGGTGCTTGAATGTTGTCAAGATCGCACTCTCGACGTTGAAGCCATTAAGAACATGAAAATGGTATTCCTGCTTAATAATATCAAAACTTGGAATGATTTATTGAGTTACAAAGAATTCAATTCTTATTATCTTAATTAATTTCTTATTGCCTATTGTAGTTAACAATGGTGATTCAAGAGACACTTAGGTTGTACCCACCAGGAGTTTTTATCACAAGAGAAGCATTGGAAGACATAAGATTAAAAAACATCATGATTCCAAAAGGGATGACTTTTCAAATTTCAATCTCGTTGCTACATCATAACGTTGATATATGGGGCCCGAACGCGCTCACATTCGATCTCCAAAGGTTTAGTAATGGCATCCTCAAAGCTTGCAAGAATGCACATGCTTACATACCGTTTGGAGTCGGACCTCACATCTGCGCGGGCCAACATCTTGCAATGCTAGAACTAAAAGTGATTGTGAGCCTTATTGTGTCAAAATTTGAATTCTCTATTTCACCTTCTTATAAGCATTCCCCTACTTTCAGCTTGGTTGTGGAGCCTAAAAATGGAGTTATTCTCAAACTAAGGAAGCTTTGA

mRNA sequence

ATGGCGGCAATGATGGTACTCTCTGTAATAGCCGTTATTTTCTTCTTCATTTTTCTTCACCTCTTTGAATCGCTGTTCTTGAAACCGGAAAGACTACGATCGAAGCTCCGTCGACAAGGAATCAAGGGTCCTTCCCCTTCTTTTCTCCTCGGAAACATACCGGAAATTAAGAACATTAGAAGCTTAAAATCATTTGGTGAAGAAGACGATTCGATTGCTCATGGTTGGTCTTCCAATCTGCTTCCCCATTTGGAGCACTGGCGGAATCGATACGGAAGAAACTTCGTGTATTCGAGCGGGACGGTTCAAATATTGTGCATAACGGACGTTGAGATGGTGAAGGAAATTGGTATGTCAACGACGTTAAGTTTGGGAAAGCCTGCCCACCTTTCCAAGGATCGTGGGCCGTTATTGGGCCTCGGCATTTTGGCATCAAATGGCCCACTTTGGGACATGACAAAGCTTATGGTGGAATCTGTAAACTCTTTGGTAAAATCATGGGAAACCATAATTGACAATGGTAGAGGAGAATCAGAAATCAACGTGGATGGATATTTTAGAGTCATGTCTGCTGATGTTATCTCGAAAGCCTGTTTCGGAAGTAATTTTTATGAGGGGAAAGAGATATTTCAAAAACTCAAAGCTCTTCAAATTATCATGTCCAAAGCAAGCATTGGCATTCCTGGGTTCAGGTATCTCCCCACCAAAAATAATAGAGAAATATGGAAGCTTGAGAAGGAAGTAGAATCAATGGTTTTAGATGTGGTAAACAAACGAATCAAGCAATGCTCAAATGAAAAAGACTTCGACAAGTTCATTGTTGATAACTGCAAAAACATATACTTTGCTGGCCACGAGACAACATCAATAATGGCATCATGGTGCTTGTTGTTATTAGCAATACACCAAGACTGGCAAGCTCGTGTTCGTTCCGAGGTGCTTGAATGTTGTCAAGATCGCACTCTCGACGTTGAAGCCATTAAGAACATGAAAATGTTAACAATGGTGATTCAAGAGACACTTAGGTTGTACCCACCAGGAGTTTTTATCACAAGAGAAGCATTGGAAGACATAAGATTAAAAAACATCATGATTCCAAAAGGGATGACTTTTCAAATTTCAATCTCGTTGCTACATCATAACGTTGATATATGGGGCCCGAACGCGCTCACATTCGATCTCCAAAGGTTTAGTAATGGCATCCTCAAAGCTTGCAAGAATGCACATGCTTACATACCGTTTGGAGTCGGACCTCACATCTGCGCGGGCCAACATCTTGCAATGCTAGAACTAAAAGTGATTGTGAGCCTTATTGTGTCAAAATTTGAATTCTCTATTTCACCTTCTTATAAGCATTCCCCTACTTTCAGCTTGGTTGTGGAGCCTAAAAATGGAGTTATTCTCAAACTAAGGAAGCTTTGA

Coding sequence (CDS)

ATGGCGGCAATGATGGTACTCTCTGTAATAGCCGTTATTTTCTTCTTCATTTTTCTTCACCTCTTTGAATCGCTGTTCTTGAAACCGGAAAGACTACGATCGAAGCTCCGTCGACAAGGAATCAAGGGTCCTTCCCCTTCTTTTCTCCTCGGAAACATACCGGAAATTAAGAACATTAGAAGCTTAAAATCATTTGGTGAAGAAGACGATTCGATTGCTCATGGTTGGTCTTCCAATCTGCTTCCCCATTTGGAGCACTGGCGGAATCGATACGGAAGAAACTTCGTGTATTCGAGCGGGACGGTTCAAATATTGTGCATAACGGACGTTGAGATGGTGAAGGAAATTGGTATGTCAACGACGTTAAGTTTGGGAAAGCCTGCCCACCTTTCCAAGGATCGTGGGCCGTTATTGGGCCTCGGCATTTTGGCATCAAATGGCCCACTTTGGGACATGACAAAGCTTATGGTGGAATCTGTAAACTCTTTGGTAAAATCATGGGAAACCATAATTGACAATGGTAGAGGAGAATCAGAAATCAACGTGGATGGATATTTTAGAGTCATGTCTGCTGATGTTATCTCGAAAGCCTGTTTCGGAAGTAATTTTTATGAGGGGAAAGAGATATTTCAAAAACTCAAAGCTCTTCAAATTATCATGTCCAAAGCAAGCATTGGCATTCCTGGGTTCAGGTATCTCCCCACCAAAAATAATAGAGAAATATGGAAGCTTGAGAAGGAAGTAGAATCAATGGTTTTAGATGTGGTAAACAAACGAATCAAGCAATGCTCAAATGAAAAAGACTTCGACAAGTTCATTGTTGATAACTGCAAAAACATATACTTTGCTGGCCACGAGACAACATCAATAATGGCATCATGGTGCTTGTTGTTATTAGCAATACACCAAGACTGGCAAGCTCGTGTTCGTTCCGAGGTGCTTGAATGTTGTCAAGATCGCACTCTCGACGTTGAAGCCATTAAGAACATGAAAATGTTAACAATGGTGATTCAAGAGACACTTAGGTTGTACCCACCAGGAGTTTTTATCACAAGAGAAGCATTGGAAGACATAAGATTAAAAAACATCATGATTCCAAAAGGGATGACTTTTCAAATTTCAATCTCGTTGCTACATCATAACGTTGATATATGGGGCCCGAACGCGCTCACATTCGATCTCCAAAGGTTTAGTAATGGCATCCTCAAAGCTTGCAAGAATGCACATGCTTACATACCGTTTGGAGTCGGACCTCACATCTGCGCGGGCCAACATCTTGCAATGCTAGAACTAAAAGTGATTGTGAGCCTTATTGTGTCAAAATTTGAATTCTCTATTTCACCTTCTTATAAGCATTCCCCTACTTTCAGCTTGGTTGTGGAGCCTAAAAATGGAGTTATTCTCAAACTAAGGAAGCTTTGA

Protein sequence

MAAMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTTLSLGKPAHLSKDRGPLLGLGILASNGPLWDMTKLMVESVNSLVKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKASIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDFDKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTLDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNVDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKFEFSISPSYKHSPTFSLVVEPKNGVILKLRKL
Homology
BLAST of PI0025986 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.2e-133
Identity = 248/506 (49.01%), Postives = 333/506 (65.81%), Query Frame = 0

Query: 12  VIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSL-KSFGEEDD 71
           ++   +F +++  L+L+PERLR KLR QG++GP PSFL GNIPE++ I+ L KS  E++ 
Sbjct: 17  ILCILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEA 76

Query: 72  SIAHGWSSN----LLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTTLSLGK 131
                +SSN    L P+  HW   YG  ++YS+G++Q+L +TD  MVKE+    +L LGK
Sbjct: 77  GSTDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANCKSLDLGK 136

Query: 132 PAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSLVKSWET 191
           P +L K+RG LLG+GIL SNG LW                  M  LM+E+  S++ SW+ 
Sbjct: 137 PCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKN 196

Query: 192 IIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKAS--IGI 251
            +++  G +EI VD + R  SADVIS+ACFGS+F EGKEIF K++ LQ  M+K S  IG+
Sbjct: 197 EVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGV 256

Query: 252 PGFRYLPTKNNREIWKLEKEVESMVLDV-----------VNK----RIKQCSNEKDF--- 311
           PG RYLPT++NR IW L+  + +++L++           VNK     I Q S +  F   
Sbjct: 257 PGSRYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVNKDLLHSIIQGSKDGPFASC 316

Query: 312 --DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTLDVEAI 371
             + FIVDNCKNIYFAGHETTS  A+WCL+LLA H +WQ+R R E L+ CQ R LD + +
Sbjct: 317 TPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLDFDIL 376

Query: 372 KNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNVDIWGP 431
           + +K LTMVIQETLRLYPP  F+ REAL D++L  I IPKG    I I++ H +  +WGP
Sbjct: 377 RKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPSVWGP 436

Query: 432 NALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKFEFSIS 474
           +A  FD  RF+NGI  ACK  H Y+PFGVG   CAGQ+LAM+ELKV++SL++SKFEF +S
Sbjct: 437 SADKFDPDRFANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKFEFKLS 496

BLAST of PI0025986 vs. ExPASy Swiss-Prot
Match: Q93Z79 (Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.9e-111
Identity = 207/505 (40.99%), Postives = 318/505 (62.97%), Query Frame = 0

Query: 19  LHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSLKSFGEE----DDSIAH 78
           + ++  +  +  R+R KL  QG+KGP PS   GN+PE++ I+S      +    D+ IAH
Sbjct: 25  IRVYGKVMAEQWRMRRKLTMQGVKGPPPSLFRGNVPEMQKIQSQIMSNSKHYSGDNIIAH 84

Query: 79  GWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTTLSLGKPAHLSKDR 138
            ++S+L P+L+HWR +YGR + YS+G  Q L +   E+VKE+  + TL+LGK ++++K  
Sbjct: 85  DYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANTLNLGKVSYVTKRL 144

Query: 139 GPLLGLGILASNGPLW-----------------DMTKLMVESVNSLVKSWETIID-NGRG 198
             +LG G++ SNGP W                  M  L+VES   ++  WE ++   G  
Sbjct: 145 KSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMKREGEM 204

Query: 199 ESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKASI--GIPGFR--Y 258
             +I VD   R  SADVIS+ACFGS+F +GKEIF KL+ LQ  ++  +I   + GF    
Sbjct: 205 VCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNGFTDVV 264

Query: 259 LPTK--NNREIWKLEKEVESMVLDVVNKRIKQC--SNEKD-------------------- 318
             TK   N +I +LE+ +ES++ + V +R ++C   ++KD                    
Sbjct: 265 FGTKKHGNGKIDELERHIESLIWETVKERERECVGDHKKDLMQLILEGARSSCDGNLEDK 324

Query: 319 ---FDKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTLDVE 378
              +  F+VDNCK+IYFAGHET+++  SWCL+LLA++  WQ R+R EV   C++   D +
Sbjct: 325 TQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEVFLHCKNGIPDAD 384

Query: 379 AIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNVDIW 438
           +I N+K +TMVIQETLRLYPP  F++REALED +L N+++PKG+     I  LH + +IW
Sbjct: 385 SISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHRDPEIW 444

Query: 439 GPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKFEFS 471
           G +A  F+ +RFS G+ KACK+  +++PFG+G  +C G++  M+ELKV+VSLIVS+F F+
Sbjct: 445 GADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIVSRFSFT 504

BLAST of PI0025986 vs. ExPASy Swiss-Prot
Match: Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.0e-111
Identity = 210/511 (41.10%), Postives = 317/511 (62.04%), Query Frame = 0

Query: 9   VIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSLKSFGEE 68
           ++ V  F +  H++    ++  R+R  L+ QG+KGP PS   GN+ E++ I+S       
Sbjct: 15  IVIVGIFSVGYHVYGRAVVEQWRMRRSLKLQGVKGPPPSIFNGNVSEMQRIQSEAKHCSG 74

Query: 69  DDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTTLSLGKPA 128
           D+ I+H +SS+L PH +HWR +YGR + YS+G  Q L I   EMVKE+  + TL+LG+  
Sbjct: 75  DNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNTLNLGRIT 134

Query: 129 HLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSLVKSWETII 188
           H++K   P+LG GI+ SNGP W                  M  LMVES   ++  WE ++
Sbjct: 135 HITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMV 194

Query: 189 DNGRGE--SEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKASI--GI 248
             G GE   +I VD   + +SADVI+KACFGS+F +GK IF  ++ L   ++K S+    
Sbjct: 195 KRG-GEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRSVLFRF 254

Query: 249 PGFRYLPTKNNR----EIWKLEKEVESMVLDVVNKRIKQC--SNEKD------------- 308
            GF  +   + +    +I  LE E+ES + + V +R  +C  +++KD             
Sbjct: 255 NGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAMRSC 314

Query: 309 ---------FDKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQD 368
                    + +F+VDNCK+IYFAGH++T++  SWCL+LLA++  WQ ++R E+L  C++
Sbjct: 315 DGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSSCKN 374

Query: 369 RTLDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLH 428
              D E+I N+K +TMVIQET+RLYPP   + REA +DIRL ++++PKG+     I  LH
Sbjct: 375 GIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIPALH 434

Query: 429 HNVDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIV 471
            + +IWGP+A  F  +RFS GI KACK   +YIPFG+GP  C G++  M+E+KV+VSLIV
Sbjct: 435 RDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVSLIV 494

BLAST of PI0025986 vs. ExPASy Swiss-Prot
Match: B9G934 (Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 1.0e-108
Identity = 214/512 (41.80%), Postives = 316/512 (61.72%), Query Frame = 0

Query: 1   MAAMMVLSVIAVIFFFIFL-HLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNI 60
           M  ++ L V+ VI   + L +L   L+L+  ++R KLRRQGI+GP P+FL GN  EIK I
Sbjct: 1   MEKLLALIVVLVILLSLALFYLCNILWLRAVKIRKKLRRQGIRGPKPTFLYGNTKEIKRI 60

Query: 61  RSLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMS 120
           R      ++  +  + + S L PH   WR  YG  F+YS+G ++IL ++  +MVK+IG  
Sbjct: 61  RQELKLSQKQGT--NNFISTLFPHFLLWRETYGPVFLYSTGAMEILQVSHPDMVKDIGRW 120

Query: 121 TTLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNS 180
           T   LGKP +L K R  L G G+   NG  W                  M +L+ ++   
Sbjct: 121 TPSELGKPNYLKKSRKALFGGGLFTENGDEWAYQRKIIAPEFFMDKIKGMIQLIEDATVP 180

Query: 181 LVKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQ--IIM 240
           ++++WE +ID+  G  EI VD Y R +SADVI++ACFGS+F +G+EIF KL+ LQ  I  
Sbjct: 181 VLEAWEDMIDDEGGCREIVVDDYLRNLSADVIARACFGSSFTKGEEIFCKLRQLQKAIAR 240

Query: 241 SKASIGIPG-FRYLPTKNNREIWKLEKEVESMVLDVVNKR-----------------IKQ 300
             + +G+   ++YLPTK+++EI  L+++V  ++LDV  ++                  + 
Sbjct: 241 QDSFVGLSALWKYLPTKSSQEIQMLDEQVRLLILDVAKEQHHYQDSHNSLVNAIIDGAQD 300

Query: 301 CSNEKDFDKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDR-T 360
             +  + + FIV NCK IYF GHE+T++ A WCL+LLA H +WQ R R+E +E C+ R T
Sbjct: 301 GRSAAEAEDFIVGNCKTIYFGGHESTAVTAIWCLMLLATHPEWQERARAEAMEVCRGRST 360

Query: 361 LDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHN 420
           LDV+A++ +K++TMVIQETLRLYPP   + REAL D++L +I +P+G   Q+   +LH +
Sbjct: 361 LDVDALRRLKIVTMVIQETLRLYPPASVMMREALTDVKLGSIEVPRGTIVQVPRLMLHLD 420

Query: 421 VDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSK 474
            + WG +A  F   RF+NG+  AC+ AH Y+PFG GP  C GQ+LAM ELKV+++ +++K
Sbjct: 421 KEAWGADADEFRPDRFANGVAAACRAAHMYVPFGHGPRTCIGQNLAMAELKVVLARLLTK 480

BLAST of PI0025986 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 8.2e-106
Identity = 202/514 (39.30%), Postives = 305/514 (59.34%), Query Frame = 0

Query: 1   MAAMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNI- 60
           MA  ++LS+  V    + ++L+  L+L P+++ +K   Q I GP PSF  GN+ +++   
Sbjct: 7   MAVKVLLSLCCVGACGLAVYLYHILWLVPQKVLAKFEDQKIGGPRPSFPYGNLADMREAA 66

Query: 61  -------RSLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEM 120
                   S +  G     I H +   +LP+ E WR  YG  F YS G V  L ++  ++
Sbjct: 67  AAAKAARASARRSGSGGGGIVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDV 126

Query: 121 VKEIGMSTTLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKL 180
           V++I +  +L LGK ++L     PL G GIL SNG  W                  M  L
Sbjct: 127 VRDINLCVSLDLGKSSYLKATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDL 186

Query: 181 MVESVNSLVKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKA 240
           MV+S   L+ SWE  +D   G ++I +D   R  SADVIS+ CFGS++ +GKEIF K++ 
Sbjct: 187 MVDSAQPLLMSWEERVDRNGGITDIKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRE 246

Query: 241 LQIIMSKASI--GIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKR----------IKQC 300
           LQ  +SK ++   + G R+ P+  N++ W+L K+V  ++L++V +           +   
Sbjct: 247 LQQAVSKPNVLAEMTGLRFFPSMRNKQAWELHKQVRKLILEIVKESGEDRNLLSAILHSA 306

Query: 301 SNEK----DFDKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQD 360
           S  +    + + FIVDNCK+IYFAGHE+T++ A+WCL+LL +H +WQ RVR EV E C+ 
Sbjct: 307 STSRVGIAEAENFIVDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRG 366

Query: 361 RTLDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLH 420
           + +D  +++ MK LTMVIQETLRLYP G F++R+AL++++L  + IPKG+   I +S +H
Sbjct: 367 QPVDSRSLQKMKNLTMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMH 426

Query: 421 HNVDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIV 474
            + ++WGP+   F+ +RFS+         H+Y+PFG G   C GQ  AM ELK+++SLIV
Sbjct: 427 LDPELWGPDVKEFNPERFSD----VRPQLHSYLPFGAGARTCLGQGFAMAELKILISLIV 486

BLAST of PI0025986 vs. ExPASy TrEMBL
Match: A0A5D3DD37 (Cytochrome P450 714C2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold275G00890 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 9.1e-241
Identity = 427/512 (83.40%), Postives = 447/512 (87.30%), Query Frame = 0

Query: 1   MAAMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIR 60
           MAAM++LSVIAVIFFF+FLHLFE LFLKPERLRSKLRRQGI GPSPSFLLGNIPEIK IR
Sbjct: 1   MAAMILLSVIAVIFFFLFLHLFELLFLKPERLRSKLRRQGIDGPSPSFLLGNIPEIKTIR 60

Query: 61  SLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMST 120
           SLKSFGEE+ SIAHGWSSNLL HLEHWRNRYGRNFVYSSGTVQ+LCITDVEMVKEIGMST
Sbjct: 61  SLKSFGEEEGSIAHGWSSNLLSHLEHWRNRYGRNFVYSSGTVQMLCITDVEMVKEIGMST 120

Query: 121 TLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSL 180
           TLSLGKPAH SKDRGPLLGLGILAS+GPLW                 DMT LMVESVNS+
Sbjct: 121 TLSLGKPAHFSKDRGPLLGLGILASSGPLWVHQRKTIAPPLYLDKVKDMTNLMVESVNSM 180

Query: 181 VKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKA 240
           VKSWETII+NG GESEINVDGYFR MSAD+ISKACFGSNF EGKEIFQKL+ALQIIMSKA
Sbjct: 181 VKSWETIIENGGGESEINVDGYFRAMSADIISKACFGSNFNEGKEIFQKLRALQIIMSKA 240

Query: 241 SIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF-------------- 300
           SIGIPGFRYLPTKNNR+IWKLEKEVE MVLDVVNKRI+QCSNEKD               
Sbjct: 241 SIGIPGFRYLPTKNNRQIWKLEKEVELMVLDVVNKRIEQCSNEKDLLQIIIEGGKCLNED 300

Query: 301 --------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRT 360
                   DKFIVDNCKNIYFAGHETTSI  SWCL+LLAIHQDWQARVRSEVLECCQDR 
Sbjct: 301 GNSLKISRDKFIVDNCKNIYFAGHETTSITTSWCLMLLAIHQDWQARVRSEVLECCQDRA 360

Query: 361 LDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHN 420
           LDVE IKNMK LTMVIQETLRLYPPGVFITREALEDIR KNI+IPKG+  QI ISLLHH+
Sbjct: 361 LDVETIKNMKTLTMVIQETLRLYPPGVFITREALEDIRFKNIVIPKGVNLQIPISLLHHS 420

Query: 421 VDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSK 474
           VDIWGPNAL FD QRFSNGILKACKN HAYIPFGVGPHICAGQHLAM+ELKVIVSLIVSK
Sbjct: 421 VDIWGPNALLFDPQRFSNGILKACKNPHAYIPFGVGPHICAGQHLAMVELKVIVSLIVSK 480

BLAST of PI0025986 vs. ExPASy TrEMBL
Match: A0A1S4E273 (cytochrome P450 714C2-like OS=Cucumis melo OX=3656 GN=LOC103496956 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 9.1e-241
Identity = 427/512 (83.40%), Postives = 447/512 (87.30%), Query Frame = 0

Query: 1   MAAMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIR 60
           MAAM++LSVIAVIFFF+FLHLFE LFLKPERLRSKLRRQGI GPSPSFLLGNIPEIK IR
Sbjct: 1   MAAMILLSVIAVIFFFLFLHLFELLFLKPERLRSKLRRQGIDGPSPSFLLGNIPEIKTIR 60

Query: 61  SLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMST 120
           SLKSFGEE+ SIAHGWSSNLL HLEHWRNRYGRNFVYSSGTVQ+LCITDVEMVKEIGMST
Sbjct: 61  SLKSFGEEEGSIAHGWSSNLLSHLEHWRNRYGRNFVYSSGTVQMLCITDVEMVKEIGMST 120

Query: 121 TLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSL 180
           TLSLGKPAH SKDRGPLLGLGILAS+GPLW                 DMT LMVESVNS+
Sbjct: 121 TLSLGKPAHFSKDRGPLLGLGILASSGPLWVHQRKTIAPPLYLDKVKDMTNLMVESVNSM 180

Query: 181 VKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKA 240
           VKSWETII+NG GESEINVDGYFR MSAD+ISKACFGSNF EGKEIFQKL+ALQIIMSKA
Sbjct: 181 VKSWETIIENGGGESEINVDGYFRAMSADIISKACFGSNFNEGKEIFQKLRALQIIMSKA 240

Query: 241 SIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF-------------- 300
           SIGIPGFRYLPTKNNR+IWKLEKEVE MVLDVVNKRI+QCSNEKD               
Sbjct: 241 SIGIPGFRYLPTKNNRQIWKLEKEVELMVLDVVNKRIEQCSNEKDLLQIIIEGGKCLNED 300

Query: 301 --------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRT 360
                   DKFIVDNCKNIYFAGHETTSI  SWCL+LLAIHQDWQARVRSEVLECCQDR 
Sbjct: 301 GNSLKISRDKFIVDNCKNIYFAGHETTSITTSWCLMLLAIHQDWQARVRSEVLECCQDRA 360

Query: 361 LDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHN 420
           LDVE IKNMK LTMVIQETLRLYPPGVFITREALEDIR KNI+IPKG+  QI ISLLHH+
Sbjct: 361 LDVETIKNMKTLTMVIQETLRLYPPGVFITREALEDIRFKNIVIPKGVNLQIPISLLHHS 420

Query: 421 VDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSK 474
           VDIWGPNAL FD QRFSNGILKACKN HAYIPFGVGPHICAGQHLAM+ELKVIVSLIVSK
Sbjct: 421 VDIWGPNALLFDPQRFSNGILKACKNPHAYIPFGVGPHICAGQHLAMVELKVIVSLIVSK 480

BLAST of PI0025986 vs. ExPASy TrEMBL
Match: A0A0A0LI16 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G120940 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.7e-192
Identity = 337/512 (65.82%), Postives = 407/512 (79.49%), Query Frame = 0

Query: 3   AMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSL 62
           A+M+L++I ++FFFI LHLF+S F KP+ LRSKLR+QGI GP PS LLGN+ +IKN+R+L
Sbjct: 12  AVMLLTLIPLLFFFISLHLFKSYFWKPQTLRSKLRKQGIDGPPPSSLLGNLSQIKNLRAL 71

Query: 63  --KSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMST 122
             ++   E +SI H W+SNL PHLE WRNRYGRNFVYSSGT+QILCIT++E VKEI + T
Sbjct: 72  TPQTKSTEHNSITHAWTSNLFPHLELWRNRYGRNFVYSSGTIQILCITEMETVKEISLWT 131

Query: 123 TLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSL 182
           +LSLGKP HLSKDRGPLLGLGILAS+GP+W                  MT LMVES NS+
Sbjct: 132 SLSLGKPDHLSKDRGPLLGLGILASSGPIWVHQRKIIAPQLYLDKVKGMTNLMVESANSM 191

Query: 183 VKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKA 242
           ++SWE  ++N  G+SEINVD   R +SAD+ISKACFGSN+ EGKEIF KL+ALQ++MSK 
Sbjct: 192 LRSWEIKVENDGGQSEINVDDDLRALSADIISKACFGSNYSEGKEIFLKLRALQVVMSKG 251

Query: 243 SIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF-------------- 302
           SIGIPGFRY+PTKNNREIW+LEKE+ES+VL+VVN+R ++ S+EKD               
Sbjct: 252 SIGIPGFRYIPTKNNREIWRLEKEIESIVLNVVNERSERSSHEKDLLQMILEGAKSLEED 311

Query: 303 --------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRT 362
                   DKFIVDNCKNIYFAGHETT+I ASWCL+LLA H DWQARVRSEVL+CCQDR 
Sbjct: 312 NNSLNISRDKFIVDNCKNIYFAGHETTAITASWCLMLLAAHPDWQARVRSEVLQCCQDRP 371

Query: 363 LDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHN 422
           ++ +AIKNMKMLTMVIQETLRLYPP  F+TR+ALEDI+LKNI IPKGM  QI I +L  +
Sbjct: 372 INADAIKNMKMLTMVIQETLRLYPPAAFVTRQALEDIKLKNITIPKGMNVQIPIPILQQD 431

Query: 423 VDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSK 474
           + IWGP+A +FD QRF+NGI +ACKN  AY+PFGVGP +CAGQ+ AM+ELKVIVSL+VS+
Sbjct: 432 IHIWGPDAHSFDPQRFNNGIARACKNPQAYMPFGVGPRVCAGQNFAMVELKVIVSLVVSR 491

BLAST of PI0025986 vs. ExPASy TrEMBL
Match: A0A6J1DSL7 (cytochrome P450 714C2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022789 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 8.6e-191
Identity = 334/511 (65.36%), Postives = 395/511 (77.30%), Query Frame = 0

Query: 2   AAMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRS 61
           A M  LS+ A++ F I LHLFES    P+R RSKLR+QGI GP PSFLLGN+ EIKNIR+
Sbjct: 9   ATMAALSLAALLLFSISLHLFESFLWNPKRRRSKLRKQGIDGPPPSFLLGNLSEIKNIRA 68

Query: 62  LKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTT 121
           L S   ED SI+H W SNL PHLE WRNRYG  FVY SGT+QILC+T++E VKEI +ST+
Sbjct: 69  LTSGIAEDGSISHAWPSNLFPHLEQWRNRYGAMFVYWSGTIQILCVTEMETVKEICLSTS 128

Query: 122 LSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSLV 181
           LSLGKPAHLSKDRGPLLGLGILAS+GP+W                  MT LMVES +S++
Sbjct: 129 LSLGKPAHLSKDRGPLLGLGILASSGPIWVHQRKIIAPSLYLDKVKGMTSLMVESTSSML 188

Query: 182 KSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKAS 241
           +SWE+ ++N  G SEINVDG  R +SAD+ISKACFGSN+ EGKEIF KL+ALQ++MSK S
Sbjct: 189 RSWESRVENDGGRSEINVDGDLRALSADIISKACFGSNYSEGKEIFLKLRALQVVMSKGS 248

Query: 242 IGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF--------------- 301
           IG+PGFRY+PTKNNRE+WKLEKE+ESMVL VVN+RI+  S+++D                
Sbjct: 249 IGVPGFRYIPTKNNREMWKLEKEIESMVLKVVNERIEHSSHDQDLLQMILEGAESLGKDD 308

Query: 302 -------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTL 361
                  DKFIVDNCKNIYFAGHETT+I ASWCL+LLA H DWQAR RSEVL+CCQDR +
Sbjct: 309 KSLNISRDKFIVDNCKNIYFAGHETTAITASWCLMLLAAHPDWQARARSEVLQCCQDRPI 368

Query: 362 DVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNV 421
           D + +KNMK LTMVIQETLRLYPP  F+TREALEDIRLK++ IPKG   QI I +L  + 
Sbjct: 369 DADTVKNMKTLTMVIQETLRLYPPAAFVTREALEDIRLKSLTIPKGTNVQIPIPMLQQDF 428

Query: 422 DIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKF 474
           D+WGP+A +FD QRFSNGIL+ACKN  AYIPFGVGP +CAGQH AM+ELKVIVSL++S+F
Sbjct: 429 DLWGPDACSFDPQRFSNGILRACKNPLAYIPFGVGPRVCAGQHFAMVELKVIVSLVLSRF 488

BLAST of PI0025986 vs. ExPASy TrEMBL
Match: A0A5D3BRT1 (Cytochrome P450 714C2-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold775G00310 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 2.1e-189
Identity = 332/512 (64.84%), Postives = 405/512 (79.10%), Query Frame = 0

Query: 3   AMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSL 62
           A+MVL+++ ++FF I LHLF+S F KP+ LRSKLR+QGI GP PS LLGN+ EIKNIR+L
Sbjct: 2   AVMVLTLMPLLFFLISLHLFQSYFWKPKTLRSKLRKQGIDGPPPSSLLGNLSEIKNIRAL 61

Query: 63  KS--FGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMST 122
            S    ++ DSI HGW+S L PHLE WRN+YG+NFVYSSGT+QILCIT++E VKEI + T
Sbjct: 62  TSQTKSKQHDSITHGWTSILFPHLELWRNQYGQNFVYSSGTIQILCITEMETVKEISLWT 121

Query: 123 TLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSL 182
           +L LGKP HLSKDRGPLLGLGILAS+GP+W                  MT LMVES N++
Sbjct: 122 SLGLGKPDHLSKDRGPLLGLGILASSGPIWVHQRKIIAPQLYLDKVKGMTSLMVESTNAM 181

Query: 183 VKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKA 242
           ++SWE  ++N  G+SEINVD   R +SAD+ISKACFGSN+ EGKEIF KL++LQ++MSK 
Sbjct: 182 LRSWEIKVENDGGQSEINVDDDLRALSADIISKACFGSNYSEGKEIFLKLRSLQVVMSKG 241

Query: 243 SIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF-------------- 302
           SIGIPGFRY+PTKNNREIW+LEKE+ES+VL+VVN+R  Q S+EKD               
Sbjct: 242 SIGIPGFRYIPTKNNREIWRLEKEIESIVLNVVNERNGQSSHEKDLLQMILEGAKSLEED 301

Query: 303 --------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRT 362
                   DKFIVDNCKNIYFAGHETT+I ASWCL+LLA H DWQARVRSEVL+CCQDR 
Sbjct: 302 GNSLKISRDKFIVDNCKNIYFAGHETTAITASWCLMLLAAHPDWQARVRSEVLQCCQDRP 361

Query: 363 LDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHN 422
           ++ +AIKNMKMLTMVIQETLRLYPP VF+TR+A+EDI+LKNI IPKGM  QI I +L  +
Sbjct: 362 INADAIKNMKMLTMVIQETLRLYPPAVFVTRQAMEDIKLKNITIPKGMNVQIPIPILQQD 421

Query: 423 VDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSK 474
           + +WGP+A +F+ QRF+NGI+KACKN  AY+PFGVGP +CAGQ+ AM+ELK+IV L+VS+
Sbjct: 422 IHLWGPDAHSFNPQRFNNGIVKACKNPQAYMPFGVGPRVCAGQNFAMVELKLIVGLVVSR 481

BLAST of PI0025986 vs. NCBI nr
Match: XP_016902075.1 (PREDICTED: cytochrome P450 714C2-like [Cucumis melo] >KAA0033319.1 cytochrome P450 714C2-like [Cucumis melo var. makuwa] >TYK21587.1 cytochrome P450 714C2-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 842.4 bits (2175), Expect = 1.9e-240
Identity = 427/512 (83.40%), Postives = 447/512 (87.30%), Query Frame = 0

Query: 1   MAAMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIR 60
           MAAM++LSVIAVIFFF+FLHLFE LFLKPERLRSKLRRQGI GPSPSFLLGNIPEIK IR
Sbjct: 1   MAAMILLSVIAVIFFFLFLHLFELLFLKPERLRSKLRRQGIDGPSPSFLLGNIPEIKTIR 60

Query: 61  SLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMST 120
           SLKSFGEE+ SIAHGWSSNLL HLEHWRNRYGRNFVYSSGTVQ+LCITDVEMVKEIGMST
Sbjct: 61  SLKSFGEEEGSIAHGWSSNLLSHLEHWRNRYGRNFVYSSGTVQMLCITDVEMVKEIGMST 120

Query: 121 TLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSL 180
           TLSLGKPAH SKDRGPLLGLGILAS+GPLW                 DMT LMVESVNS+
Sbjct: 121 TLSLGKPAHFSKDRGPLLGLGILASSGPLWVHQRKTIAPPLYLDKVKDMTNLMVESVNSM 180

Query: 181 VKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKA 240
           VKSWETII+NG GESEINVDGYFR MSAD+ISKACFGSNF EGKEIFQKL+ALQIIMSKA
Sbjct: 181 VKSWETIIENGGGESEINVDGYFRAMSADIISKACFGSNFNEGKEIFQKLRALQIIMSKA 240

Query: 241 SIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF-------------- 300
           SIGIPGFRYLPTKNNR+IWKLEKEVE MVLDVVNKRI+QCSNEKD               
Sbjct: 241 SIGIPGFRYLPTKNNRQIWKLEKEVELMVLDVVNKRIEQCSNEKDLLQIIIEGGKCLNED 300

Query: 301 --------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRT 360
                   DKFIVDNCKNIYFAGHETTSI  SWCL+LLAIHQDWQARVRSEVLECCQDR 
Sbjct: 301 GNSLKISRDKFIVDNCKNIYFAGHETTSITTSWCLMLLAIHQDWQARVRSEVLECCQDRA 360

Query: 361 LDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHN 420
           LDVE IKNMK LTMVIQETLRLYPPGVFITREALEDIR KNI+IPKG+  QI ISLLHH+
Sbjct: 361 LDVETIKNMKTLTMVIQETLRLYPPGVFITREALEDIRFKNIVIPKGVNLQIPISLLHHS 420

Query: 421 VDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSK 474
           VDIWGPNAL FD QRFSNGILKACKN HAYIPFGVGPHICAGQHLAM+ELKVIVSLIVSK
Sbjct: 421 VDIWGPNALLFDPQRFSNGILKACKNPHAYIPFGVGPHICAGQHLAMVELKVIVSLIVSK 480

BLAST of PI0025986 vs. NCBI nr
Match: XP_004139011.2 (cytochrome P450 714C2 isoform X1 [Cucumis sativus])

HSP 1 Score: 839.3 bits (2167), Expect = 1.6e-239
Identity = 426/511 (83.37%), Postives = 444/511 (86.89%), Query Frame = 0

Query: 2   AAMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRS 61
           AAM+VL VIAVI FFIFLHLFESLFLKPERLRSKLRRQGI GPSPSF LGNIPEIKNIRS
Sbjct: 30  AAMVVLYVIAVICFFIFLHLFESLFLKPERLRSKLRRQGIDGPSPSFFLGNIPEIKNIRS 89

Query: 62  LKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTT 121
           LKSF E++DSIAHGWSSNLLPHLEHWR RYGRNFVYSSGTVQILCITDVEMVKEIGMST 
Sbjct: 90  LKSFDEKEDSIAHGWSSNLLPHLEHWRYRYGRNFVYSSGTVQILCITDVEMVKEIGMSTN 149

Query: 122 LSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSLV 181
           LSLGKPAH SKDRGPLLGLGILAS+GPLW                 DMT LMVESVNS+V
Sbjct: 150 LSLGKPAHFSKDRGPLLGLGILASSGPLWVHQRKTIAPQLYLDKVKDMTNLMVESVNSMV 209

Query: 182 KSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKAS 241
           K WETII+NG GESEINVDGYFR MSAD+ISKACFGSNFYEGKEIFQKL+ALQIIMS A+
Sbjct: 210 KLWETIIENGGGESEINVDGYFRAMSADIISKACFGSNFYEGKEIFQKLRALQIIMSTAT 269

Query: 242 IGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF--------------- 301
           IGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKD                
Sbjct: 270 IGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDLLQIIIEGGKCLNKDG 329

Query: 302 -------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTL 361
                  DKFIVDNCKNIYFAGHETTSI  +WCL+LLAIHQDWQ RVRSEVLECCQDRTL
Sbjct: 330 NSLKISRDKFIVDNCKNIYFAGHETTSITTTWCLMLLAIHQDWQTRVRSEVLECCQDRTL 389

Query: 362 DVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNV 421
           DVE IKNMK LTMVIQETLRLYPPGVFITREALEDI+ KNI IPKGM  QI ISLLHH+V
Sbjct: 390 DVETIKNMKTLTMVIQETLRLYPPGVFITREALEDIKFKNITIPKGMNLQIPISLLHHSV 449

Query: 422 DIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKF 474
           DIWGP+ALTF+ QRFSNGILKACKN HAYIPFGVGPHICAGQHLAM+ELKVIVS+IVSKF
Sbjct: 450 DIWGPDALTFNPQRFSNGILKACKNPHAYIPFGVGPHICAGQHLAMVELKVIVSVIVSKF 509

BLAST of PI0025986 vs. NCBI nr
Match: XP_031737081.1 (cytochrome P450 714C2 isoform X2 [Cucumis sativus] >KGN61505.2 hypothetical protein Csa_006493 [Cucumis sativus])

HSP 1 Score: 836.3 bits (2159), Expect = 1.3e-238
Identity = 424/509 (83.30%), Postives = 442/509 (86.84%), Query Frame = 0

Query: 4   MMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSLK 63
           M+VL VIAVI FFIFLHLFESLFLKPERLRSKLRRQGI GPSPSF LGNIPEIKNIRSLK
Sbjct: 1   MVVLYVIAVICFFIFLHLFESLFLKPERLRSKLRRQGIDGPSPSFFLGNIPEIKNIRSLK 60

Query: 64  SFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTTLS 123
           SF E++DSIAHGWSSNLLPHLEHWR RYGRNFVYSSGTVQILCITDVEMVKEIGMST LS
Sbjct: 61  SFDEKEDSIAHGWSSNLLPHLEHWRYRYGRNFVYSSGTVQILCITDVEMVKEIGMSTNLS 120

Query: 124 LGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSLVKS 183
           LGKPAH SKDRGPLLGLGILAS+GPLW                 DMT LMVESVNS+VK 
Sbjct: 121 LGKPAHFSKDRGPLLGLGILASSGPLWVHQRKTIAPQLYLDKVKDMTNLMVESVNSMVKL 180

Query: 184 WETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKASIG 243
           WETII+NG GESEINVDGYFR MSAD+ISKACFGSNFYEGKEIFQKL+ALQIIMS A+IG
Sbjct: 181 WETIIENGGGESEINVDGYFRAMSADIISKACFGSNFYEGKEIFQKLRALQIIMSTATIG 240

Query: 244 IPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF----------------- 303
           IPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKD                  
Sbjct: 241 IPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDLLQIIIEGGKCLNKDGNS 300

Query: 304 -----DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTLDV 363
                DKFIVDNCKNIYFAGHETTSI  +WCL+LLAIHQDWQ RVRSEVLECCQDRTLDV
Sbjct: 301 LKISRDKFIVDNCKNIYFAGHETTSITTTWCLMLLAIHQDWQTRVRSEVLECCQDRTLDV 360

Query: 364 EAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNVDI 423
           E IKNMK LTMVIQETLRLYPPGVFITREALEDI+ KNI IPKGM  QI ISLLHH+VDI
Sbjct: 361 ETIKNMKTLTMVIQETLRLYPPGVFITREALEDIKFKNITIPKGMNLQIPISLLHHSVDI 420

Query: 424 WGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKFEF 474
           WGP+ALTF+ QRFSNGILKACKN HAYIPFGVGPHICAGQHLAM+ELKVIVS+IVSKFEF
Sbjct: 421 WGPDALTFNPQRFSNGILKACKNPHAYIPFGVGPHICAGQHLAMVELKVIVSVIVSKFEF 480

BLAST of PI0025986 vs. NCBI nr
Match: XP_038906772.1 (cytochrome P450 714C2-like [Benincasa hispida])

HSP 1 Score: 693.0 bits (1787), Expect = 1.8e-195
Identity = 347/513 (67.64%), Postives = 409/513 (79.73%), Query Frame = 0

Query: 3   AMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSP-SFLLGNIPEIKNIRS 62
           A+MVLS IA++ F I LHLFESL  KPERLRSKLR+QGI GPSP S L GN+ EIKNIR+
Sbjct: 12  AVMVLS-IALLLFLISLHLFESLLWKPERLRSKLRKQGIDGPSPSSSLFGNLSEIKNIRA 71

Query: 63  LKS--FGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMS 122
           L S     EDDSI H W+SNL PHLE WRNRYGRNFVYSSGT+QILCIT++E VKE+ +S
Sbjct: 72  LTSQTKNTEDDSITHNWTSNLFPHLELWRNRYGRNFVYSSGTIQILCITEMETVKEMSLS 131

Query: 123 TTLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNS 182
           T+LSLGKP HLSKDRGPLLGLGILAS+GP+W                  MT LMVES NS
Sbjct: 132 TSLSLGKPDHLSKDRGPLLGLGILASSGPIWVHQRKIIAPQLYLDKVKGMTSLMVESTNS 191

Query: 183 LVKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSK 242
           +++SWET + N  G+SE+NVDG FR +SAD+ISKACFGSN+ EGKEIFQKL+ALQ++MSK
Sbjct: 192 MLRSWETKVVNDGGQSEVNVDGDFRALSADIISKACFGSNYLEGKEIFQKLRALQVVMSK 251

Query: 243 ASIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF------------- 302
            SIGIPGFRY+PTKNNREIWKLEKE++S VL+VVN+RI+  S+E+D              
Sbjct: 252 GSIGIPGFRYVPTKNNREIWKLEKEIKSKVLEVVNQRIEHSSHEQDLLQMILEGAKNLDE 311

Query: 303 ---------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDR 362
                    DKF+VDNCKNIYFAGHETT+I ASWCL+LLA H DWQ RVRSEVL+CCQDR
Sbjct: 312 DNNSLNISRDKFVVDNCKNIYFAGHETTAITASWCLMLLAAHPDWQVRVRSEVLQCCQDR 371

Query: 363 TLDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHH 422
            ++ +AIKNMKMLTMV+QETLRLYPP VF+TR+A+EDI+ KNIMIPKGM  QI I ++  
Sbjct: 372 PINADAIKNMKMLTMVVQETLRLYPPAVFVTRQAMEDIKFKNIMIPKGMNVQIPIPIVQQ 431

Query: 423 NVDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVS 474
           +VD+WGP+A  F+ QRF NGIL+ACKN  AY+PFGVGP +C GQ+ AM+ELKVIVSL+VS
Sbjct: 432 DVDLWGPDAHLFNPQRFDNGILRACKNPQAYMPFGVGPRVCVGQNFAMVELKVIVSLVVS 491

BLAST of PI0025986 vs. NCBI nr
Match: XP_004138935.1 (cytochrome P450 714C2 [Cucumis sativus] >KGN61428.1 hypothetical protein Csa_006371 [Cucumis sativus])

HSP 1 Score: 681.4 bits (1757), Expect = 5.5e-192
Identity = 337/512 (65.82%), Postives = 407/512 (79.49%), Query Frame = 0

Query: 3   AMMVLSVIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSL 62
           A+M+L++I ++FFFI LHLF+S F KP+ LRSKLR+QGI GP PS LLGN+ +IKN+R+L
Sbjct: 12  AVMLLTLIPLLFFFISLHLFKSYFWKPQTLRSKLRKQGIDGPPPSSLLGNLSQIKNLRAL 71

Query: 63  --KSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMST 122
             ++   E +SI H W+SNL PHLE WRNRYGRNFVYSSGT+QILCIT++E VKEI + T
Sbjct: 72  TPQTKSTEHNSITHAWTSNLFPHLELWRNRYGRNFVYSSGTIQILCITEMETVKEISLWT 131

Query: 123 TLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSL 182
           +LSLGKP HLSKDRGPLLGLGILAS+GP+W                  MT LMVES NS+
Sbjct: 132 SLSLGKPDHLSKDRGPLLGLGILASSGPIWVHQRKIIAPQLYLDKVKGMTNLMVESANSM 191

Query: 183 VKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKA 242
           ++SWE  ++N  G+SEINVD   R +SAD+ISKACFGSN+ EGKEIF KL+ALQ++MSK 
Sbjct: 192 LRSWEIKVENDGGQSEINVDDDLRALSADIISKACFGSNYSEGKEIFLKLRALQVVMSKG 251

Query: 243 SIGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCSNEKDF-------------- 302
           SIGIPGFRY+PTKNNREIW+LEKE+ES+VL+VVN+R ++ S+EKD               
Sbjct: 252 SIGIPGFRYIPTKNNREIWRLEKEIESIVLNVVNERSERSSHEKDLLQMILEGAKSLEED 311

Query: 303 --------DKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRT 362
                   DKFIVDNCKNIYFAGHETT+I ASWCL+LLA H DWQARVRSEVL+CCQDR 
Sbjct: 312 NNSLNISRDKFIVDNCKNIYFAGHETTAITASWCLMLLAAHPDWQARVRSEVLQCCQDRP 371

Query: 363 LDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHN 422
           ++ +AIKNMKMLTMVIQETLRLYPP  F+TR+ALEDI+LKNI IPKGM  QI I +L  +
Sbjct: 372 INADAIKNMKMLTMVIQETLRLYPPAAFVTRQALEDIKLKNITIPKGMNVQIPIPILQQD 431

Query: 423 VDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSK 474
           + IWGP+A +FD QRF+NGI +ACKN  AY+PFGVGP +CAGQ+ AM+ELKVIVSL+VS+
Sbjct: 432 IHIWGPDAHSFDPQRFNNGIARACKNPQAYMPFGVGPRVCAGQNFAMVELKVIVSLVVSR 491

BLAST of PI0025986 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 403.7 bits (1036), Expect = 2.1e-112
Identity = 207/505 (40.99%), Postives = 318/505 (62.97%), Query Frame = 0

Query: 19  LHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSLKSFGEE----DDSIAH 78
           + ++  +  +  R+R KL  QG+KGP PS   GN+PE++ I+S      +    D+ IAH
Sbjct: 25  IRVYGKVMAEQWRMRRKLTMQGVKGPPPSLFRGNVPEMQKIQSQIMSNSKHYSGDNIIAH 84

Query: 79  GWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTTLSLGKPAHLSKDR 138
            ++S+L P+L+HWR +YGR + YS+G  Q L +   E+VKE+  + TL+LGK ++++K  
Sbjct: 85  DYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANTLNLGKVSYVTKRL 144

Query: 139 GPLLGLGILASNGPLW-----------------DMTKLMVESVNSLVKSWETIID-NGRG 198
             +LG G++ SNGP W                  M  L+VES   ++  WE ++   G  
Sbjct: 145 KSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMKREGEM 204

Query: 199 ESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKASI--GIPGFR--Y 258
             +I VD   R  SADVIS+ACFGS+F +GKEIF KL+ LQ  ++  +I   + GF    
Sbjct: 205 VCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNGFTDVV 264

Query: 259 LPTK--NNREIWKLEKEVESMVLDVVNKRIKQC--SNEKD-------------------- 318
             TK   N +I +LE+ +ES++ + V +R ++C   ++KD                    
Sbjct: 265 FGTKKHGNGKIDELERHIESLIWETVKERERECVGDHKKDLMQLILEGARSSCDGNLEDK 324

Query: 319 ---FDKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTLDVE 378
              +  F+VDNCK+IYFAGHET+++  SWCL+LLA++  WQ R+R EV   C++   D +
Sbjct: 325 TQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEVFLHCKNGIPDAD 384

Query: 379 AIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNVDIW 438
           +I N+K +TMVIQETLRLYPP  F++REALED +L N+++PKG+     I  LH + +IW
Sbjct: 385 SISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHRDPEIW 444

Query: 439 GPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKFEFS 471
           G +A  F+ +RFS G+ KACK+  +++PFG+G  +C G++  M+ELKV+VSLIVS+F F+
Sbjct: 445 GADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIVSRFSFT 504

BLAST of PI0025986 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 402.9 bits (1034), Expect = 3.5e-112
Identity = 210/511 (41.10%), Postives = 317/511 (62.04%), Query Frame = 0

Query: 9   VIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSLKSFGEE 68
           ++ V  F +  H++    ++  R+R  L+ QG+KGP PS   GN+ E++ I+S       
Sbjct: 15  IVIVGIFSVGYHVYGRAVVEQWRMRRSLKLQGVKGPPPSIFNGNVSEMQRIQSEAKHCSG 74

Query: 69  DDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTTLSLGKPA 128
           D+ I+H +SS+L PH +HWR +YGR + YS+G  Q L I   EMVKE+  + TL+LG+  
Sbjct: 75  DNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNTLNLGRIT 134

Query: 129 HLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNSLVKSWETII 188
           H++K   P+LG GI+ SNGP W                  M  LMVES   ++  WE ++
Sbjct: 135 HITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMV 194

Query: 189 DNGRGE--SEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKASI--GI 248
             G GE   +I VD   + +SADVI+KACFGS+F +GK IF  ++ L   ++K S+    
Sbjct: 195 KRG-GEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRSVLFRF 254

Query: 249 PGFRYLPTKNNR----EIWKLEKEVESMVLDVVNKRIKQC--SNEKD------------- 308
            GF  +   + +    +I  LE E+ES + + V +R  +C  +++KD             
Sbjct: 255 NGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAMRSC 314

Query: 309 ---------FDKFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQD 368
                    + +F+VDNCK+IYFAGH++T++  SWCL+LLA++  WQ ++R E+L  C++
Sbjct: 315 DGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSSCKN 374

Query: 369 RTLDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLH 428
              D E+I N+K +TMVIQET+RLYPP   + REA +DIRL ++++PKG+     I  LH
Sbjct: 375 GIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIPALH 434

Query: 429 HNVDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIV 471
            + +IWGP+A  F  +RFS GI KACK   +YIPFG+GP  C G++  M+E+KV+VSLIV
Sbjct: 435 RDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVSLIV 494

BLAST of PI0025986 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 303.1 bits (775), Expect = 3.8e-82
Identity = 176/513 (34.31%), Postives = 283/513 (55.17%), Query Frame = 0

Query: 1   MAAMMVLSVIAVIFFFIFLHLFESL---FLKPERLRSKLRRQGIKGPSPSFLLGNIPEIK 60
           M  +++  V+ ++   I   L++S+   FL P R++  + RQGI GP P  L GNI +I 
Sbjct: 2   MVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDIS 61

Query: 61  NIRSLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIG 120
            + S  S   +  SI H     LLPH   W  +YG+ F+  +GT   LC+T+ EM+KE+ 
Sbjct: 62  KMLS-HSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 121

Query: 121 MSTTLSLGKPAHLSKDRGPLLGLGILASNGPLWDMTKLMVES----------VNSLVKSW 180
                  GK     +     +G G+L +NG  W   + M                +V+  
Sbjct: 122 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 181

Query: 181 ETIIDNGRGE--SEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSKAS- 240
           + + +  R E   E+ +    R ++AD+IS+  FGS+  +GKE+F  L  LQ + ++A+ 
Sbjct: 182 KMMAERLRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATR 241

Query: 241 -IGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKR----------------IKQCSNEK 300
            +  PG R+LP+K NREI  L+ EVE +++++++ R                +    N+ 
Sbjct: 242 HLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQM 301

Query: 301 DFDK------FIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECC-QDR 360
           D +K       I+D CK  +F GHETTS++ +W L+LLA +  WQ  VR EV + C QD 
Sbjct: 302 DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDG 361

Query: 361 TLDVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHH 420
              VE + ++  L  VI E+LRLYPP   + R A EDI+L +++IPKG++  I +  +HH
Sbjct: 362 VPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHH 421

Query: 421 NVDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVS 474
           + ++WG +A  F+ +RF+    ++  ++  ++PF  GP  C GQ  AM+E K+I++++VS
Sbjct: 422 SNELWGEDANEFNPERFTT---RSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVS 481

BLAST of PI0025986 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 295.4 bits (755), Expect = 8.0e-80
Identity = 176/521 (33.78%), Postives = 283/521 (54.32%), Query Frame = 0

Query: 4   MMVLSVIAVIFFFIFLH-LFESL---FLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNI 63
           + +L  + VIF    L  L++++   +L P R++  + +QG+ GP P  L GNI EI  +
Sbjct: 3   LTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAM 62

Query: 64  RSLKSFGEEDDSIAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMS 123
            S +S  ++ DSI H     LLPH   W  +YG+ F+  +GT   LC+T+ E++KE+ M 
Sbjct: 63  VS-QSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMK 122

Query: 124 TTLSLGKPAHLSKDRGPLLGLGILASNGPLW-----------------DMTKLMVESVNS 183
                G+     +     +G G+L +NG  W                    + MVE  + 
Sbjct: 123 HNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSK 182

Query: 184 LVKSWETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIMSK 243
           LV+     +  G G +E+ +      ++AD+IS+  FGS+F +GKE+F  L  LQ   ++
Sbjct: 183 LVERLRKEV--GEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQ 242

Query: 244 AS--IGIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKRIKQCS---------------- 303
           A+  +  PG R+LP+K NREI  L+KEVE ++++++  R + C+                
Sbjct: 243 ATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSR-RDCAEMGRSSTHGDDLLGLL 302

Query: 304 -NEKDFDK----------FIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEV 363
            NE D DK           I+D CK  +FAGHETT+++ +W  +LLA +  WQ +VR EV
Sbjct: 303 LNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEV 362

Query: 364 LECCQDRTL-DVEAIKNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQ 423
            E      L  V+ +  +  L+ VI E+LRLYPP   + R A ED++L ++ IPKG++  
Sbjct: 363 REVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIW 422

Query: 424 ISISLLHHNVDIWGPNALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELK 474
           I +  +HH+ ++WG +A  F+ +RF     +   +   +IPF  GP  C GQ  A++E K
Sbjct: 423 IPVLAIHHSEELWGKDANQFNPERFGG---RPFASGRHFIPFAAGPRNCIGQQFALMEAK 482

BLAST of PI0025986 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 285.8 bits (730), Expect = 6.3e-77
Identity = 160/506 (31.62%), Postives = 257/506 (50.79%), Query Frame = 0

Query: 9   VIAVIFFFIFLHLFESLFLKPERLRSKLRRQGIKGPSPSFLLGNIPEIKNIRSLKSFGEE 68
           V  V+   +FL LF   ++ P R + KLR  G  GP+PSF  GN+ ++K ++      + 
Sbjct: 16  VFVVVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDN 75

Query: 69  DDS---IAHGWSSNLLPHLEHWRNRYGRNFVYSSGTVQILCITDVEMVKEIGMSTT-LSL 128
             S   I H   S  LPH   W+  YG+ FVY  G    + + D E +  +       S 
Sbjct: 76  SKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSW 135

Query: 129 GKPAHLSKDRGPLLGLGILASNGPLWD-----------------MTKLMVESVNSLVKSW 188
           GKP    KDR P+ G G++   G  W                  MT +MVESV++++  W
Sbjct: 136 GKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRW 195

Query: 189 ETIIDNGRGESEINVDGYFRVMSADVISKACFGSNFYEGKEIFQKLKALQIIM--SKASI 248
              I++  G  E +++      + ++I+K  FG     G ++ + L+A+Q  +  S   +
Sbjct: 196 GIQINS--GNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRYV 255

Query: 249 GIPGFRYLPTKNNREIWKLEKEVESMVLDVVNKR-----------------IKQCSNEKD 308
           G+P    L  K   +   L  E++ ++L  +NKR                 + +   + +
Sbjct: 256 GVPFSNILSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLGMLLKADQKGN 315

Query: 309 FD-KFIVDNCKNIYFAGHETTSIMASWCLLLLAIHQDWQARVRSEVLECCQDRTLDVEAI 368
           F  K +VD CK  +FAGHETT++  +W  +LLAIH +WQ  +R E+ E   D  ++   +
Sbjct: 316 FTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREVIGDSKIEYNKL 375

Query: 369 KNMKMLTMVIQETLRLYPPGVFITREALEDIRLKNIMIPKGMTFQISISLLHHNVDIWGP 428
             +K ++ V+ E LRLYPP     R+A  DI +   +IP G    I +  +HH+V++WG 
Sbjct: 376 AGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDVELWGD 435

Query: 429 NALTFDLQRFSNGILKACKNAHAYIPFGVGPHICAGQHLAMLELKVIVSLIVSKFEFSIS 474
           +   F  +RF   +   CKN   Y+PFG G  +C G++L  +E K+++SL++S+FE S+S
Sbjct: 436 DVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSRFEISVS 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2QYH71.2e-13349.01Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
Q93Z792.9e-11140.99Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1[more]
Q6NKZ85.0e-11141.10Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1[more]
B9G9341.0e-10841.80Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 ... [more]
B6SSW88.2e-10639.30Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DD379.1e-24183.40Cytochrome P450 714C2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S4E2739.1e-24183.40cytochrome P450 714C2-like OS=Cucumis melo OX=3656 GN=LOC103496956 PE=3 SV=1[more]
A0A0A0LI162.7e-19265.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G120940 PE=3 SV=1[more]
A0A6J1DSL78.6e-19165.36cytochrome P450 714C2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A5D3BRT12.1e-18964.84Cytochrome P450 714C2-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_016902075.11.9e-24083.40PREDICTED: cytochrome P450 714C2-like [Cucumis melo] >KAA0033319.1 cytochrome P4... [more]
XP_004139011.21.6e-23983.37cytochrome P450 714C2 isoform X1 [Cucumis sativus][more]
XP_031737081.11.3e-23883.30cytochrome P450 714C2 isoform X2 [Cucumis sativus] >KGN61505.2 hypothetical prot... [more]
XP_038906772.11.8e-19567.64cytochrome P450 714C2-like [Benincasa hispida][more]
XP_004138935.15.5e-19265.82cytochrome P450 714C2 [Cucumis sativus] >KGN61428.1 hypothetical protein Csa_006... [more]
Match NameE-valueIdentityDescription
AT5G24910.12.1e-11240.99cytochrome P450, family 714, subfamily A, polypeptide 1 [more]
AT5G24900.13.5e-11241.10cytochrome P450, family 714, subfamily A, polypeptide 2 [more]
AT1G67110.13.8e-8234.31cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT5G38450.18.0e-8033.78cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT5G52400.16.3e-7731.62cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 335..346
score: 47.33
coord: 412..421
score: 50.88
coord: 283..300
score: 42.02
coord: 421..432
score: 45.03
IPR001128Cytochrome P450PFAMPF00067p450coord: 84..464
e-value: 6.3E-62
score: 209.8
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 330..346
score: 43.21
coord: 274..300
score: 23.08
coord: 405..421
score: 39.59
coord: 421..439
score: 32.79
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..473
e-value: 7.3E-89
score: 300.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 38..472
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 7..468
NoneNo IPR availablePANTHERPTHR24282:SF26CYTOCHROME P450 FAMILY PROTEINcoord: 7..468
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 414..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025986.1PI0025986.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen