PI0025840 (gene) Melon (PI 482460) v1

BLAST of PI0025840 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 7.3e-261
Identity = 459/772 (59.46%), Postives = 563/772 (72.93%), Query Frame = 0

Query: 1   MKISNSILILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIF 60
           M + +   +L  + L  SPA A KKSYIV LGSH+H  +++   L+ V  SH+  L S  
Sbjct: 16  MSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFV 75

Query: 61  RSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMH 120
            S E A++AIFYSYK++INGFAAI+DE EA ++AKHP+V +V PN+ ++LHTTHSW FM 
Sbjct: 76  GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFML 135

Query: 121 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTP 180
           L KNGV+  SS W +A  G+D IIANLDTGVWPESKSF + G  G VP++WKG C     
Sbjct: 136 LAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRC----H 195

Query: 181 DGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANV 240
             VPCNRKLIGA+YFNKG+LAY    +  +    + RD+DGHGSHTLSTA G++V GANV
Sbjct: 196 KDVPCNRKLIGARYFNKGYLAYTGLPSNAS--YETCRDHDGHGSHTLSTAAGNFVPGANV 255

Query: 241 FGSGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLSLGGEP 300
           FG G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S+GG+ 
Sbjct: 256 FGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDA 315

Query: 301 AEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVE 360
            +Y  DGIAI +FHAVK G+ VVCSAGNSGP + TVSN APW++TVGAS++DREFQA VE
Sbjct: 316 GDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVE 375

Query: 361 LQNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVC 420
           L+NG  + G+SLSK L   K+Y LI+ A+A   N     A+LCK  +LD  KVKGKILVC
Sbjct: 376 LKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVC 435

Query: 421 LRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYINS 480
           LRGD ARVDKG QAA AGA GM+LCND+ SG E I+D HVLPAS I+Y DG+ ++SY++S
Sbjct: 436 LRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSS 495

Query: 481 TKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSP 540
           TK+P GY+  P+A +NTKPAP MA+FSSRGPN I+P I+KPD+TAPG NIIAAF+EA  P
Sbjct: 496 TKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGP 555

Query: 541 TGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNK 600
           T    DNR  PF T SGTSMSCPH+SG+VGLL+TLHP WSP+AI+SAIMT++R R+N  K
Sbjct: 556 TDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRK 615

Query: 601 PMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFS 660
           PM+D        A PF+YGSGH++P  A  PGLVYDL+  DYL+FLCA GY+   +Q F+
Sbjct: 616 PMVDE---SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 675

Query: 661 -DGHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVK 720
            D  + C   A++L+FNYPSI V NL GS+TVTRKLKNV  P  Y AR R P GV++ V+
Sbjct: 676 EDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVE 735

Query: 721 PKVLKFERVGEEKSFELTITG-DVPENQVVDGVLIWTDGKHFVRSPIVVSSS 770
           PK L F + GE K F++T+    V  +  V G L WTD  H+VRSPIVV  S
Sbjct: 736 PKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

BLAST of PI0025840 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 2.7e-231
Identity = 414/778 (53.21%), Postives = 547/778 (70.31%), Query Frame = 0

Query: 1   MKISNSILILFSLLLI--ISPAIAAKK---SYIVLLGSHSHGLEVTDESLERVVDSHKKL 60
           MK++++   L  LLL+   S  I A K   SY+V  G+HSH  E+T+++++RV ++H   
Sbjct: 1   MKLTHNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDF 60

Query: 61  LGSIFRSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHS 120
           LGS   S E+A DAIFYSY K+INGFAA +D + A +++KHPEV +V PN+A +LHTT S
Sbjct: 61  LGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRS 120

Query: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
           W+F+ LE N  +P SS WR+A+ G+D IIANLDTGVWPESKSF + G+ GP+PS+WKG C
Sbjct: 121 WDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGIC 180

Query: 181 TDKTPDGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYV 240
            ++      CNRKLIGA+YFNKG+ A +   N   S  +S RD DGHGSHTLSTA G +V
Sbjct: 181 QNQKDATFHCNRKLIGARYFNKGYAAAVGHLN---SSFDSPRDLDGHGSHTLSTAAGDFV 240

Query: 241 SGANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLS 300
            G ++FG G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+S
Sbjct: 241 PGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVS 300

Query: 301 LGGEPAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREF 360
           LGGEP  ++ D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGAST+DREF
Sbjct: 301 LGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREF 360

Query: 361 QAPVELQNGHRYMGSSLSK-GLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVK 420
            + + L NG  Y G SLS   L  +K YP++    AKAKNA+A  A LCK  +LD  K K
Sbjct: 361 ASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTK 420

Query: 421 GKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAV 480
           GKILVCLRG   RV+KG   AL G +GM+L N  ++G + +ADPHVLPA+ +   D  AV
Sbjct: 421 GKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAV 480

Query: 481 YSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAF 540
             YI+ TK P+ ++ P    +  KPAP MA+FSS+GP+ ++P+I+KPD+TAPG ++IAA+
Sbjct: 481 SRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAY 540

Query: 541 SEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARI 600
           + AVSPT E FD R + F  +SGTSMSCPH+SG+ GLL+T +P WSP+AI+SAIMT+A I
Sbjct: 541 TGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATI 600

Query: 601 RDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEK 660
            D+   P+ +  +++   ATPF++G+GH++P  A++PGLVYDL   DYL FLC+ GY+  
Sbjct: 601 MDDIPGPIQNATNMK---ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNAS 660

Query: 661 TIQAFSDGHFKCPA-SASILNFNYPSIGVQNLNGS-VTVTRKLKNVSTPGVYKARIRHPN 720
            I  FS  +F C +   S++N NYPSI V NL  S VTV+R +KNV  P +Y  ++ +P 
Sbjct: 661 QISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQ 720

Query: 721 GVKILVKPKVLKFERVGEEKSFELTIT---GDVPENQVVDGVLIWTDGKHFVRSPIVV 767
           GV + VKP  L F +VGE+K+F++ +    G+V +  V  G L+W+D KH VRSPIVV
Sbjct: 721 GVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

BLAST of PI0025840 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 656.8 bits (1693), Expect = 3.1e-187
Identity = 383/787 (48.67%), Postives = 491/787 (62.39%), Query Frame = 0

Query: 8   LILFSLLL---IISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIFRSDE 67
           LIL S  L   +++    +KK YIV +G+HSHG   T   LE   DSH  LLGSIF S E
Sbjct: 10  LILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSRE 69

Query: 68  KARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMHLEKN 127
           KA++AI YSY ++INGFAA+++EEEA  +AK+P V +V  ++  +LHTT SWEF+ L + 
Sbjct: 70  KAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR 129

Query: 128 GVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC--TDKTPDG 187
           G    +SAW++ + G++ II N+DTGVWPES+SF + G  G VPSKW+GG    +K P  
Sbjct: 130 G---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQINKLPGS 189

Query: 188 V--PCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANV 247
           +   CNRKLIGA+Y+NK F A+         ++++ RD+ GHG+HTLSTAGG++V GA V
Sbjct: 190 MKNTCNRKLIGARYYNKAFEAH---NGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARV 249

Query: 248 FGSGVGTAKGGSPKARVAAYKVCWPLED-GGCFDADIAEAFDHAIHDRVDVLSLSLGGE- 307
           F  G GTAKGGSP+ARVAAYKVCW L D   C+ AD+  A D AI D VDV+++S G   
Sbjct: 250 FAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSY 309

Query: 308 --PAE-YYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQ 367
              AE  + D I+I AFHA+ K I +V SAGN GP   TV+N APW+ T+ ASTLDR+F 
Sbjct: 310 VVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFS 369

Query: 368 APVELQNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK 427
           + + + N     G+SL   L  ++ + LI   +AK  NAT   A LC+  TLD +KV GK
Sbjct: 370 SNLTI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGK 429

Query: 428 ILVCLR-GDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVL-----PASHINYND 487
           I++C R G    V +G +A  AGA GMIL N   +G    A+PHV      P        
Sbjct: 430 IVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRP 489

Query: 488 GQAVYSYINSTKNPM--GYLIPPS---AKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTA 547
                + I    +P+  G  I  S        KPAP MA+FSSRGPN I P I+KPDVTA
Sbjct: 490 HGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTA 549

Query: 548 PGANIIAAFSEAVSPTGEPFDNRT-VPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAI 607
           PG NI+AA+SE  S +    DNR    F  + GTSMSCPH SG+ GLL+T HP WSP+AI
Sbjct: 550 PGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAI 609

Query: 608 KSAIMTSARIRDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLE 667
           KSAIMT+A   DNTN+P+ D     LA A  FAYGSGH+RP  AI+PGLVYDLS  DYL 
Sbjct: 610 KSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLTDYLN 669

Query: 668 FLCASGYDEKTIQAFS-DGHFKCPASASILNFNYPSIGVQNLN-GSVTVTRKLKNVSTPG 727
           FLCASGYD++ I A + +  F C  S S+ + NYPSI + NL    VT+ R + NV  P 
Sbjct: 670 FLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPS 729

Query: 728 VYKARIRHPNGVKILVKPKVLKFERVGEEKSFELTI--TGDVPENQVVDGVLIWTDGKHF 767
            Y    R PNG  I V P  L F ++GE K+F++ +  +      +   G L WTDGKH 
Sbjct: 730 TYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHI 786

BLAST of PI0025840 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 5.7e-165
Identity = 333/727 (45.80%), Postives = 447/727 (61.49%), Query Frame = 0

Query: 50  DSHKKLLGSIFRSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQ 109
           D H     S  RS   + + + Y+Y+  I+GF+  + +EEA  L   P V +VLP    +
Sbjct: 46  DLHSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYE 105

Query: 110 LHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPS 169
           LHTT +  F+ L+++     +  +  A S  DV++  LDTGVWPESKS+ + G  GP+PS
Sbjct: 106 LHTTRTPLFLGLDEH----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPS 165

Query: 170 KWKGGCTDKTP-DGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLS 229
            WKGGC   T      CNRKLIGA++F +G+ + +   +  +    S RD DGHG+HT S
Sbjct: 166 SWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPID-ESKESRSPRDDDGHGTHTSS 225

Query: 230 TAGGSYVSGANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRV 289
           TA GS V GA++ G   GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V
Sbjct: 226 TAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNV 285

Query: 290 DVLSLSLGGEPAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGAS 349
           +VLS+SLGG  ++YY DG+AI AF A+++GI V CSAGN+GP + ++SN APWI TVGA 
Sbjct: 286 NVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAG 345

Query: 350 TLDREFQAPVELQNGHRYMGSSLSKG-LKGSKLYPLITGAEAKAKNATAEVAMLCKPKTL 409
           TLDR+F A   L NG  + G SL KG     KL P I      A NAT     LC   TL
Sbjct: 346 TLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTL 405

Query: 410 DHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINY 469
              KVKGKI++C RG  ARV KG+    AG VGMIL N   +G E +AD H+LPA+ +  
Sbjct: 406 IPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGE 465

Query: 470 NDGQAVYSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGA 529
             G  +  Y+ +  NP   +      V  KP+P +AAFSSRGPN I+P I+KPD+ APG 
Sbjct: 466 KAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGV 525

Query: 530 NIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAI 589
           NI+AA++ A  PTG   D+R V F  +SGTSMSCPHVSGL  LL+++HP+WSP+AI+SA+
Sbjct: 526 NILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSAL 585

Query: 590 MTSARIRDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 649
           MT+A       KP+LD  + +  P+TPF +G+GH+ PT A +PGL+YDL+  DYL FLCA
Sbjct: 586 MTTAYKTYKDGKPLLDIATGK--PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCA 645

Query: 650 SGYDEKTIQAFSDGHFKCPASA--SILNFNYPSIGVQNLN--GSVTVTRKLKNVSTPGVY 709
             Y    I++ S  ++ C  S   S+ + NYPS  V N++  G+   TR + +V   G Y
Sbjct: 646 LNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTY 705

Query: 710 KARI-RHPNGVKILVKPKVLKFERVGEEKSFELTITGD--VPENQVVDGVLIWTDGKHFV 768
             ++     GVKI V+P VL F+   E+KS+ +T T D   P      G + W+DGKH V
Sbjct: 706 SVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVV 755

BLAST of PI0025840 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 6.4e-156
Identity = 325/784 (41.45%), Postives = 458/784 (58.42%), Query Frame = 0

Query: 11  FSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIFRSDEKARDAI 70
           F LL + SP+ +A  S      S ++ + V  E+   +  +H     S   S   +  +I
Sbjct: 8   FFLLTLSSPSSSASSS-----NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSI 67

Query: 71  FYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMHL---EKNGVI 130
            ++Y    +GF+A +  ++A+QL  HP V +V+P + + LHTT S EF+ L   +K G++
Sbjct: 68  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 127

Query: 131 PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC--TDKTPDGVPC 190
             S        G D++I  +DTGVWPE  SF + G+ GPVP KWKG C  +   P+   C
Sbjct: 128 EESD------FGSDLVIGVIDTGVWPERPSFDDRGL-GPVPIKWKGQCIASQDFPESA-C 187

Query: 191 NRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANVFGSGV 250
           NRKL+GA++F  G+ A     N T     S RD DGHG+HT S + G YV  A+  G   
Sbjct: 188 NRKLVGARFFCGGYEATNGKMNETTE-FRSPRDSDGHGTHTASISAGRYVFPASTLGYAH 247

Query: 251 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPAEYYED 310
           G A G +PKAR+AAYKVCW   + GC+D+DI  AFD A+ D VDV+SLS+GG    YY D
Sbjct: 248 GVAAGMAPKARLAAYKVCW---NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLD 307

Query: 311 GIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVELQNGHR 370
            IAI AF A+ +GI V  SAGN GPGA TV+N APW+ TVGA T+DR+F A V+L NG  
Sbjct: 308 AIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKM 367

Query: 371 YMGSSL--SKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGD 430
             G S+    GL   ++YPL+ G      +  +  + LC   +LD + VKGKI++C RG 
Sbjct: 368 ISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS--SSLCLEGSLDPNLVKGKIVLCDRGI 427

Query: 431 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYIN----- 490
            +R  KGE     G +GMI+ N    G   +AD HVLPA+ +  + G  +  YI+     
Sbjct: 428 NSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKS 487

Query: 491 -STKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAV 550
            S+K+P   ++    ++  +PAP +A+FS+RGPNP +PEI+KPDV APG NI+AA+ + +
Sbjct: 488 RSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRI 547

Query: 551 SPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNT 610
            P+G   DNR   F  +SGTSM+CPHVSGL  LL+  HP WSP+AI+SA++T+A   DN+
Sbjct: 548 GPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNS 607

Query: 611 NKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQA 670
            +PM+D  +     ++   YGSGH+ PT A+DPGLVYD++  DY+ FLC S Y    I  
Sbjct: 608 GEPMMDESTGN--TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVT 667

Query: 671 FSDGHFKCPA---SASILNFNYPSIGV--QNLNGSVTVTRKLKNVSTPG----VYKARIR 730
            +     C     +  + N NYPS  V  Q    S   T  ++ V+  G    VY+ +IR
Sbjct: 668 ITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIR 727

Query: 731 HPNGVKILVKPKVLKFERVGEEKSFELTI-TGDVP----ENQVVDGVLIWTDGKHFVRSP 768
            P G  + V+P+ L F RVG++ SF + + T +V        V  G ++W+DGK  V SP
Sbjct: 728 PPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSP 770

BLAST of PI0025840 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 723/771 (93.77%), Postives = 741/771 (96.11%), Query Frame = 0

Query: 1   MKISNSILILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIF 60
           MKI NSILILFSLLL+ISPAIAAKKSYIVLLGSHSHGLEV DE LERV DSH KLLGSI 
Sbjct: 6   MKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSII 65

Query: 61  RSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMH 120
            SDEKARDAIFYSYK+NINGFAAIMDEEEATQLAKHPEVAAVL N+AK+LHTTHSWEFMH
Sbjct: 66  GSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMH 125

Query: 121 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTP 180
           LEKNGVIPPSSAWRRAKSGKDVII NLDTGVW ESKSFGEHGIVG VPSKWKGGCTDKTP
Sbjct: 126 LEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTP 185

Query: 181 DGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANV 240
           DGV CNRKLIGAKYFNKGFLAYL S+NLTASVINSTRDYDGHGSHTLSTAGGSYVSGA+V
Sbjct: 186 DGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGSHTLSTAGGSYVSGASV 245

Query: 241 FGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPA 300
           FG GVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIAEAFDHAIHDRVDVLSLSLGGEPA
Sbjct: 246 FGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGGEPA 305

Query: 301 EYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVEL 360
           +YY+DGIAIAAFHAVKKGIPVVCSAGNSGP AQTVSNTAPWILTVGASTLDREFQAPVEL
Sbjct: 306 DYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAPVEL 365

Query: 361 QNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL 420
           QNGH YMGSSLSKGLKG KLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL
Sbjct: 366 QNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL 425

Query: 421 RGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYINST 480
           RGDTARVDKGEQAALAGAVGMILCND+LSGFETIADPHVLPASHINYNDGQAV+SYI ST
Sbjct: 426 RGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSYIKST 485

Query: 481 KNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSPT 540
           KNPMG LIPPSAKVNTKPAP+MAAFSSRGPN ISPEIIKPDVTAPG NIIAAFSEAVSPT
Sbjct: 486 KNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPT 545

Query: 541 GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNKP 600
           GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIKSAIMTSARIRDNT KP
Sbjct: 546 GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTKKP 605

Query: 601 MLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFSD 660
           MLDGGS +LAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY+EKTIQAFSD
Sbjct: 606 MLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSD 665

Query: 661 GHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVKPK 720
           G FKCPASASILNFNYPSIGVQNL GSVT+TRKLKNVSTPGVYKAR+ HPNGVK+LVKPK
Sbjct: 666 GPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLVKPK 725

Query: 721 VLKFERVGEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           VLKFERVGEEK FEL ITGDVPENQVVDGVLIWTDGKHFVRSPIVVSS LF
Sbjct: 726 VLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of PI0025840 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 711/764 (93.06%), Postives = 733/764 (95.94%), Query Frame = 0

Query: 8   LILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIFRSDEKAR 67
           ++LF L+L         +SYIVLLGSHSHGLEVTDE L+RV DSH KLLGSIF SDEKAR
Sbjct: 1   MVLFCLML-------GFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKAR 60

Query: 68  DAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMHLEKNGVI 127
           +AIFYSYKKNINGFAAIMDEEEA QLAKHPEVAAVLPNRAK+LHTTHSWEFMHLEKNGVI
Sbjct: 61  NAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVI 120

Query: 128 PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTPDGVPCNR 187
           PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT D VPCNR
Sbjct: 121 PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNR 180

Query: 188 KLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANVFGSGVGT 247
           KLIGAKYFNKGFLAYLKSENLTA VINSTRDYDGHGSHTLSTAGGSYVSGA+VFG GVGT
Sbjct: 181 KLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGT 240

Query: 248 AKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPAEYYEDGI 307
           AKGGSPKARVAAYKVCWPLEDGGCFDADIA+AFDHAIHDRVDVLSLSLGGEPA+YY+DGI
Sbjct: 241 AKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGI 300

Query: 308 AIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVELQNGHRYM 367
           AI+AFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGAST+DREFQAPVELQNGHRYM
Sbjct: 301 AISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYM 360

Query: 368 GSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARV 427
           GSSLSKGLKG KLYPLITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGDTARV
Sbjct: 361 GSSLSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARV 420

Query: 428 DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYINSTKNPMGYL 487
           DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAV+SYI +TKNPMGYL
Sbjct: 421 DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYL 480

Query: 488 IPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSPTGEPFDNR 547
           IPP+AKVNTKPAPTMAAFSSRGPN ISPEIIKPDVTAPG NIIAAFSEAVSPTGEPFDNR
Sbjct: 481 IPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNR 540

Query: 548 TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNKPMLDGGSL 607
           TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNT KPMLDGGS 
Sbjct: 541 TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSP 600

Query: 608 ELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFSDGHFKCPA 667
           +LAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY+EKTIQAFSDG FKCPA
Sbjct: 601 DLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPA 660

Query: 668 SASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVKPKVLKFERV 727
           SASILN NYPSIGVQNL GSVTVTRKLKNVSTPGVYK R+RHPNGVK+LVKPKVLKFERV
Sbjct: 661 SASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERV 720

Query: 728 GEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           GEEKSFELTITGDVPE+QVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 721 GEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of PI0025840 vs. ExPASy TrEMBL
Match: A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 617/779 (79.20%), Postives = 693/779 (88.96%), Query Frame = 0

Query: 1   MKISNSILILFSLL----LIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLL 60
           MK+SNSIL +FS L    L+ISPAIAAKKSY+V+LGSHSHGLEV++  L+RVVDSH KLL
Sbjct: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60

Query: 61  GSIFRSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSW 120
           GS   S EKA+DAIFYSYKKNINGFAAI++EEEA +LAKHPEVAAVL N+ K+LHTTHSW
Sbjct: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120

Query: 121 EFMHLEK-NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
            FM LEK NGV+PP+S W RA  G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGC
Sbjct: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180

Query: 181 TDKTPDGVPCNRKLIGAKYFNKGFLAYLKSENLTA---SVINSTRDYDGHGSHTLSTAGG 240
           TD +PD VPCNRKLIGAKYFNKGF+AYLK+ N +A   S+ NSTRDY+GHGSHTLSTAGG
Sbjct: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240

Query: 241 SYVSGANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLS 300
           S+V  A+VFGSG+GTAKGGSPKARVAAYK+CWP +DGGCFDADI E FDHAIHD V+V+S
Sbjct: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300

Query: 301 LSLGGEPAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDR 360
           LS+GG PA Y++D IAIAAFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGASTLDR
Sbjct: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360

Query: 361 EFQAPVELQNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKV 420
           +FQAPVEL+NG R+ GSSLS  L   KLYPLITGA+AKA+NA+A  AMLCKP+TLDHSK 
Sbjct: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420

Query: 421 KGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA 480
           KGKIL CLRGD ARVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQA
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480

Query: 481 VYSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAA 540
           V  YI ST+NPMGYLIPP+AK+NTKPAP MAAFSSRGPN ++PEIIKPDVTAPG N+IAA
Sbjct: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540

Query: 541 FSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSAR 600
           ++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSAR
Sbjct: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600

Query: 601 IRDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDE 660
           IRDNT KPMLDGG+ ELAPATPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GYDE
Sbjct: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660

Query: 661 KTIQAFSDGHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNG 720
           K I+AFSDG +KCP SAS+LNFNYPSIGVQN+ GSVTVTR+LKNV TPGVY+AR+R P G
Sbjct: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720

Query: 721 VKILVKPKVLKFERVGEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           V++ V+P+ LKF++VGEEKSF+LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of PI0025840 vs. ExPASy TrEMBL
Match: A0A0A0LYF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1)

HSP 1 Score: 1251.1 bits (3236), Expect = 0.0e+00
Identity = 619/775 (79.87%), Postives = 684/775 (88.26%), Query Frame = 0

Query: 1   MKISNSILILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIF 60
           MK   S LI FS LL+ISPAIA KKSY+VLLGSHSHGL+ T++  +RVVDSH KLLGS  
Sbjct: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60

Query: 61  RSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMH 120
           RS+EKA+DAIFYSYKKNINGFAA +D+E+AT+LA HPEVAAVLPN+AK L+TTHSWEFMH
Sbjct: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120

Query: 121 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCT-DKT 180
           LEKNGVIPPSS W RAK GKDVIIANLDTGVWPESKSFGEHGIVGP PSKWKGGCT DKT
Sbjct: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180

Query: 181 PDGVPCNRKLIGAKYFNKGFLAYLKSENLT---ASVINSTRDYDGHGSHTLSTAGGSYVS 240
           PDGVPCN+KLIGAKYFNKG+  YLKSEN T   +S+INSTRDY+GHGSHTLSTAGG+YV 
Sbjct: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240

Query: 241 GANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLG 300
           GA+VFGSG+GTAKGGSPKARVAAYKVCWP E GGCFDADI EAFDHAIHD VDVLSLSLG
Sbjct: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300

Query: 301 GEPAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQA 360
            +  +Y ED IAIA+FHAVKKGIPVVC+ GNSGP  +T SNTAPWILTVGASTLDREF A
Sbjct: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360

Query: 361 PVELQNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKI 420
           PV L+NG+++MGSS SKGL+G  LYPLITGA+AKA NAT + AMLCKP+TLDHSKVKGKI
Sbjct: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420

Query: 421 LVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSY 480
           LVCLRG+TAR+DKG+QAALAGAVGMILCND+LSG     D HVLPASHINY+DGQ + SY
Sbjct: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480

Query: 481 INSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEA 540
            NS + PMG LIPP A+VNTKPAPTMA FSSRGPN ISPEIIKPDVTAPG +IIAAFSEA
Sbjct: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540

Query: 541 VSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDN 600
           +SPT +P DNRT PFITMSGTSMSCPHV+GLVGLLR LHP W+PSAIKSAIMTSA++RDN
Sbjct: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600

Query: 601 TNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQ 660
           T  PMLDGGSL L PATPFAYGSGHI PTGA+DPGLVYDLSPNDYLEFLCASGYDE+TI+
Sbjct: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660

Query: 661 AFSDGHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKIL 720
           AFSD  FKCPASAS+LN NYPSIGVQNL  SVT+TRKLKNV TPGVYKA+I HPN V++ 
Sbjct: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720

Query: 721 VKPKVLKFERVGEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           VKP+ LKFERVGEEKSFELT++G VP+N+   G LIW+DG+HFVRSPIVVSS LF
Sbjct: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

BLAST of PI0025840 vs. ExPASy TrEMBL
Match: A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)

HSP 1 Score: 1246.1 bits (3223), Expect = 0.0e+00
Identity = 606/749 (80.91%), Postives = 668/749 (89.19%), Query Frame = 0

Query: 26   SYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIFRSDEKARDAIFYSYKKNINGFAAIM 85
            SY+VLLGSHSHGLEVT++  E VVDSH KLLGS  RSDEKA+D+IFYSYKK+INGFAA +
Sbjct: 755  SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814

Query: 86   DEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIA 145
            D+E+AT+LA HPEVAAVLPN+ K L+TTHSWEFMHLEKNGV+PPSS WR AK GKDVIIA
Sbjct: 815  DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 874

Query: 146  NLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTPDGVPCNRKLIGAKYFNKGFLAYLKS 205
            NLDTGVWPESKSFGEHGI GP PSKWKGGCTDK+PDGVPCN+KLIGAKYFNKG+L YLKS
Sbjct: 875  NLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKS 934

Query: 206  ENLT---ASVINSTRDYDGHGSHTLSTAGGSYVSGANVFGSGVGTAKGGSPKARVAAYKV 265
            EN T   +S+INSTRDYDGHGSHTLSTA G+YV GA+VFGSG+GTAKGGSPKARVAAYKV
Sbjct: 935  ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 994

Query: 266  CWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPAEYYEDGIAIAAFHAVKKGIPVV 325
            CWP E GGCFDADI EAFDHAIHD VDVLSLSLGG+P +Y ED IAIA+FHAVKKGIPVV
Sbjct: 995  CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 1054

Query: 326  CSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKGLKGSKLYP 385
            C+ GNSGP  +T SNTAPWILTVGASTLDREF APV LQNGHR+MGSS SKGL G KLYP
Sbjct: 1055 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 1114

Query: 386  LITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMI 445
            LITGA+AKA NA  + AMLCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAGAVGMI
Sbjct: 1115 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 1174

Query: 446  LCNDELSGFETIADPHVLPASHINYNDGQAVYSYINSTKNPMGYLIPPSAKVNTKPAPTM 505
            LCND+LSG   + D H+LPASHINY DGQ + SYINS +NPMGYLIPP AKVNTKPAPTM
Sbjct: 1175 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 1234

Query: 506  AAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCP 565
            A FSSRGPN ISPEIIKPDVTAPG NIIAAFSEA+SPT +  DNRT PFITMSGTSMSCP
Sbjct: 1235 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 1294

Query: 566  HVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNKPMLDGGSLELAPATPFAYGSGHI 625
            HV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT  PM+DGGSL+LAPATPFAYGSGHI
Sbjct: 1295 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 1354

Query: 626  RPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFSDGHFKCPASASILNFNYPSIGVQ 685
             PTGAIDPGLVYDLSPNDYLEFLCASGYDEKTI+AFSD  FKCP ++S+LN NYPSIGVQ
Sbjct: 1355 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 1414

Query: 686  NLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVKPKVLKFERVGEEKSFELTITGDVP 745
            NL GSV+VTRKLKNV +PGVY+A+I HPNGV + VKP+ LKFERVGEEKSFELT+ G VP
Sbjct: 1415 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 1474

Query: 746  ENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
            +++V  G LIW+DG+H VRSPIVVSS LF
Sbjct: 1475 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503

BLAST of PI0025840 vs. NCBI nr
Match: XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 723/771 (93.77%), Postives = 741/771 (96.11%), Query Frame = 0

Query: 1   MKISNSILILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIF 60
           MKI NSILILFSLLL+ISPAIAAKKSYIVLLGSHSHGLEV DE LERV DSH KLLGSI 
Sbjct: 6   MKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSII 65

Query: 61  RSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMH 120
            SDEKARDAIFYSYK+NINGFAAIMDEEEATQLAKHPEVAAVL N+AK+LHTTHSWEFMH
Sbjct: 66  GSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMH 125

Query: 121 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTP 180
           LEKNGVIPPSSAWRRAKSGKDVII NLDTGVW ESKSFGEHGIVG VPSKWKGGCTDKTP
Sbjct: 126 LEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTP 185

Query: 181 DGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANV 240
           DGV CNRKLIGAKYFNKGFLAYL S+NLTASVINSTRDYDGHGSHTLSTAGGSYVSGA+V
Sbjct: 186 DGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGSHTLSTAGGSYVSGASV 245

Query: 241 FGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPA 300
           FG GVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIAEAFDHAIHDRVDVLSLSLGGEPA
Sbjct: 246 FGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGGEPA 305

Query: 301 EYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVEL 360
           +YY+DGIAIAAFHAVKKGIPVVCSAGNSGP AQTVSNTAPWILTVGASTLDREFQAPVEL
Sbjct: 306 DYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAPVEL 365

Query: 361 QNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL 420
           QNGH YMGSSLSKGLKG KLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL
Sbjct: 366 QNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL 425

Query: 421 RGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYINST 480
           RGDTARVDKGEQAALAGAVGMILCND+LSGFETIADPHVLPASHINYNDGQAV+SYI ST
Sbjct: 426 RGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSYIKST 485

Query: 481 KNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSPT 540
           KNPMG LIPPSAKVNTKPAP+MAAFSSRGPN ISPEIIKPDVTAPG NIIAAFSEAVSPT
Sbjct: 486 KNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPT 545

Query: 541 GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNKP 600
           GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIKSAIMTSARIRDNT KP
Sbjct: 546 GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTKKP 605

Query: 601 MLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFSD 660
           MLDGGS +LAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY+EKTIQAFSD
Sbjct: 606 MLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSD 665

Query: 661 GHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVKPK 720
           G FKCPASASILNFNYPSIGVQNL GSVT+TRKLKNVSTPGVYKAR+ HPNGVK+LVKPK
Sbjct: 666 GPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLVKPK 725

Query: 721 VLKFERVGEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           VLKFERVGEEK FEL ITGDVPENQVVDGVLIWTDGKHFVRSPIVVSS LF
Sbjct: 726 VLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of PI0025840 vs. NCBI nr
Match: KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])

HSP 1 Score: 1433.7 bits (3710), Expect = 0.0e+00
Identity = 711/753 (94.42%), Postives = 730/753 (96.95%), Query Frame = 0

Query: 21  IAAKK--SYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIFRSDEKARDAIFYSYKKNI 80
           +AAKK  SYIVLLGSHSHGLEVTDE L+RV DSH KLLGSIF SDEKAR+AIFYSYKKNI
Sbjct: 1   MAAKKNISYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNI 60

Query: 81  NGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMHLEKNGVIPPSSAWRRAKS 140
           NGFAAIMDEEEA QLAKHPEVAAVLPNRAK+LHTTHSWEFMHLEKNGVIPPSSAWRRAKS
Sbjct: 61  NGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKS 120

Query: 141 GKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTPDGVPCNRKLIGAKYFNKG 200
           GKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT D VPCNRKLIGAKYFNKG
Sbjct: 121 GKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKG 180

Query: 201 FLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANVFGSGVGTAKGGSPKARVA 260
           FLAYLKSENLTA VINSTRDYDGHGSHTLSTAGGSYVSGA+VFG GVGTAKGGSPKARVA
Sbjct: 181 FLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVA 240

Query: 261 AYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPAEYYEDGIAIAAFHAVKKG 320
           AYKVCWPLEDGGCFDADIA+AFDHAIHDRVDVLSLSLGGEPA+YY+DGIAI+AFHAVKKG
Sbjct: 241 AYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKG 300

Query: 321 IPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKGLKGS 380
           IPVVCSAGNSGPGAQTVSNTAPWILTVGAST+DREFQAPVELQNGHRYMGSSLSKGLKG 
Sbjct: 301 IPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGD 360

Query: 381 KLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGA 440
           KLYPLITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGA
Sbjct: 361 KLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGA 420

Query: 441 VGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYINSTKNPMGYLIPPSAKVNTKP 500
           VGMILCNDELSGFETIADPHVLPASHINYNDGQAV+SYI +TKNPMGYLIPP+AKVNTKP
Sbjct: 421 VGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKP 480

Query: 501 APTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSPTGEPFDNRTVPFITMSGTS 560
           APTMAAFSSRGPN ISPEIIKPDVTAPG NIIAAFSEAVSPTGEPFDNRTVPFITMSGTS
Sbjct: 481 APTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTS 540

Query: 561 MSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNKPMLDGGSLELAPATPFAYG 620
           MSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNT KPMLDGGS +LAP+TPFAYG
Sbjct: 541 MSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYG 600

Query: 621 SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFSDGHFKCPASASILNFNYPS 680
           SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY+EKTIQAFSDG FKCPASASILN NYPS
Sbjct: 601 SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPS 660

Query: 681 IGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVKPKVLKFERVGEEKSFELTIT 740
           IGVQNL GSVTVTRKLKNVSTPGVYK R+RHPNGVK+LVKPKVLKFERVGEEKSFELTIT
Sbjct: 661 IGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTIT 720

Query: 741 GDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           GDVPE+QVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 721 GDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753

BLAST of PI0025840 vs. NCBI nr
Match: XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 711/764 (93.06%), Postives = 733/764 (95.94%), Query Frame = 0

Query: 8   LILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIFRSDEKAR 67
           ++LF L+L         +SYIVLLGSHSHGLEVTDE L+RV DSH KLLGSIF SDEKAR
Sbjct: 1   MVLFCLML-------GFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKAR 60

Query: 68  DAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMHLEKNGVI 127
           +AIFYSYKKNINGFAAIMDEEEA QLAKHPEVAAVLPNRAK+LHTTHSWEFMHLEKNGVI
Sbjct: 61  NAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVI 120

Query: 128 PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTPDGVPCNR 187
           PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT D VPCNR
Sbjct: 121 PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNR 180

Query: 188 KLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANVFGSGVGT 247
           KLIGAKYFNKGFLAYLKSENLTA VINSTRDYDGHGSHTLSTAGGSYVSGA+VFG GVGT
Sbjct: 181 KLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGT 240

Query: 248 AKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPAEYYEDGI 307
           AKGGSPKARVAAYKVCWPLEDGGCFDADIA+AFDHAIHDRVDVLSLSLGGEPA+YY+DGI
Sbjct: 241 AKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGI 300

Query: 308 AIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVELQNGHRYM 367
           AI+AFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGAST+DREFQAPVELQNGHRYM
Sbjct: 301 AISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYM 360

Query: 368 GSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARV 427
           GSSLSKGLKG KLYPLITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGDTARV
Sbjct: 361 GSSLSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARV 420

Query: 428 DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYINSTKNPMGYL 487
           DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAV+SYI +TKNPMGYL
Sbjct: 421 DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYL 480

Query: 488 IPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSPTGEPFDNR 547
           IPP+AKVNTKPAPTMAAFSSRGPN ISPEIIKPDVTAPG NIIAAFSEAVSPTGEPFDNR
Sbjct: 481 IPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNR 540

Query: 548 TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNKPMLDGGSL 607
           TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNT KPMLDGGS 
Sbjct: 541 TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSP 600

Query: 608 ELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFSDGHFKCPA 667
           +LAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY+EKTIQAFSDG FKCPA
Sbjct: 601 DLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPA 660

Query: 668 SASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVKPKVLKFERV 727
           SASILN NYPSIGVQNL GSVTVTRKLKNVSTPGVYK R+RHPNGVK+LVKPKVLKFERV
Sbjct: 661 SASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERV 720

Query: 728 GEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           GEEKSFELTITGDVPE+QVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 721 GEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of PI0025840 vs. NCBI nr
Match: XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1380.5 bits (3572), Expect = 0.0e+00
Identity = 687/773 (88.87%), Postives = 725/773 (93.79%), Query Frame = 0

Query: 1   MKISNSILILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIF 60
           MKISNSILILFS LL+IS AIAAKKSY+VLLGSHSHG+E+T+E LERVV SH KLLGS  
Sbjct: 1   MKISNSILILFSFLLLISSAIAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFL 60

Query: 61  RSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMH 120
            S+EKA+DAIFYSYKK+INGFAA +DEEEAT+LA+HPEVAA+L NRAK LHTTHSWEFMH
Sbjct: 61  GSEEKAKDAIFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMH 120

Query: 121 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTP 180
           LEKNGVIP SS WR AKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC D+TP
Sbjct: 121 LEKNGVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETP 180

Query: 181 DGVPCNRKLIGAKYFNKGFLAYLKSENLT--ASVINSTRDYDGHGSHTLSTAGGSYVSGA 240
           D VPCNRKLIGAKYFNKGF+AYLKSEN T  +SVINSTRDY+GHGSHTLSTAGGSYVSGA
Sbjct: 181 DRVPCNRKLIGAKYFNKGFIAYLKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGA 240

Query: 241 NVFGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGE 300
           +VFGSG GTAKGGSPKARVAAYKVCWPLE GGCFD+DI EAFDHAIHD VDVLSLSLG +
Sbjct: 241 SVFGSGAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSD 300

Query: 301 PAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPV 360
           PAEY ED IAIAAFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGASTLDREFQAPV
Sbjct: 301 PAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPV 360

Query: 361 ELQNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILV 420
           ELQNGHRYMGSSLSK L+G+KLYPLITGA+AKAKNA  + AMLCKP+TLDHSKVKGKIL 
Sbjct: 361 ELQNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILA 420

Query: 421 CLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYIN 480
           CLRGD ARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAV SYIN
Sbjct: 421 CLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYIN 480

Query: 481 STKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVS 540
           STKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPN ISPEIIKPDVTAPG N+IAAFSEAVS
Sbjct: 481 STKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVS 540

Query: 541 PTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTN 600
           PTGE FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIKSAIMTSARIRDNT 
Sbjct: 541 PTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTM 600

Query: 601 KPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAF 660
           KPMLDGGS +LAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYD+KTI+AF
Sbjct: 601 KPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAF 660

Query: 661 SDGHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVK 720
           SDG FKCP +ASILNFNYPSIGVQNLNG+VTVTRKLKNVSTPGVY+ARI +P+GVK+ VK
Sbjct: 661 SDGPFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVK 720

Query: 721 PKVLKFERVGEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           PKVLKFERV EEKSFELT+TG+VPE+QVVDGVLIWTDGKHFVRSPIV+SS LF
Sbjct: 721 PKVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 773

BLAST of PI0025840 vs. NCBI nr
Match: XP_022142562.1 (subtilisin-like protease SBT5.4 [Momordica charantia])

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 617/779 (79.20%), Postives = 693/779 (88.96%), Query Frame = 0

Query: 1   MKISNSILILFSLL----LIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLL 60
           MK+SNSIL +FS L    L+ISPAIAAKKSY+V+LGSHSHGLEV++  L+RVVDSH KLL
Sbjct: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60

Query: 61  GSIFRSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSW 120
           GS   S EKA+DAIFYSYKKNINGFAAI++EEEA +LAKHPEVAAVL N+ K+LHTTHSW
Sbjct: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120

Query: 121 EFMHLEK-NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
            FM LEK NGV+PP+S W RA  G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGC
Sbjct: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180

Query: 181 TDKTPDGVPCNRKLIGAKYFNKGFLAYLKSENLTA---SVINSTRDYDGHGSHTLSTAGG 240
           TD +PD VPCNRKLIGAKYFNKGF+AYLK+ N +A   S+ NSTRDY+GHGSHTLSTAGG
Sbjct: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240

Query: 241 SYVSGANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLS 300
           S+V  A+VFGSG+GTAKGGSPKARVAAYK+CWP +DGGCFDADI E FDHAIHD V+V+S
Sbjct: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300

Query: 301 LSLGGEPAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDR 360
           LS+GG PA Y++D IAIAAFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGASTLDR
Sbjct: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360

Query: 361 EFQAPVELQNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKV 420
           +FQAPVEL+NG R+ GSSLS  L   KLYPLITGA+AKA+NA+A  AMLCKP+TLDHSK 
Sbjct: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420

Query: 421 KGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA 480
           KGKIL CLRGD ARVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQA
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480

Query: 481 VYSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAA 540
           V  YI ST+NPMGYLIPP+AK+NTKPAP MAAFSSRGPN ++PEIIKPDVTAPG N+IAA
Sbjct: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540

Query: 541 FSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSAR 600
           ++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSAR
Sbjct: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600

Query: 601 IRDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDE 660
           IRDNT KPMLDGG+ ELAPATPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GYDE
Sbjct: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660

Query: 661 KTIQAFSDGHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNG 720
           K I+AFSDG +KCP SAS+LNFNYPSIGVQN+ GSVTVTR+LKNV TPGVY+AR+R P G
Sbjct: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720

Query: 721 VKILVKPKVLKFERVGEEKSFELTITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSSLF 772
           V++ V+P+ LKF++VGEEKSF+LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of PI0025840 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 901.4 bits (2328), Expect = 5.2e-262
Identity = 459/772 (59.46%), Postives = 563/772 (72.93%), Query Frame = 0

Query: 1   MKISNSILILFSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIF 60
           M + +   +L  + L  SPA A KKSYIV LGSH+H  +++   L+ V  SH+  L S  
Sbjct: 16  MSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFV 75

Query: 61  RSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMH 120
            S E A++AIFYSYK++INGFAAI+DE EA ++AKHP+V +V PN+ ++LHTTHSW FM 
Sbjct: 76  GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFML 135

Query: 121 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTP 180
           L KNGV+  SS W +A  G+D IIANLDTGVWPESKSF + G  G VP++WKG C     
Sbjct: 136 LAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRC----H 195

Query: 181 DGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANV 240
             VPCNRKLIGA+YFNKG+LAY    +  +    + RD+DGHGSHTLSTA G++V GANV
Sbjct: 196 KDVPCNRKLIGARYFNKGYLAYTGLPSNAS--YETCRDHDGHGSHTLSTAAGNFVPGANV 255

Query: 241 FGSGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLSLGGEP 300
           FG G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S+GG+ 
Sbjct: 256 FGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDA 315

Query: 301 AEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVE 360
            +Y  DGIAI +FHAVK G+ VVCSAGNSGP + TVSN APW++TVGAS++DREFQA VE
Sbjct: 316 GDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVE 375

Query: 361 LQNGHRYMGSSLSKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVC 420
           L+NG  + G+SLSK L   K+Y LI+ A+A   N     A+LCK  +LD  KVKGKILVC
Sbjct: 376 LKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVC 435

Query: 421 LRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYINS 480
           LRGD ARVDKG QAA AGA GM+LCND+ SG E I+D HVLPAS I+Y DG+ ++SY++S
Sbjct: 436 LRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSS 495

Query: 481 TKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAVSP 540
           TK+P GY+  P+A +NTKPAP MA+FSSRGPN I+P I+KPD+TAPG NIIAAF+EA  P
Sbjct: 496 TKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGP 555

Query: 541 TGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTNK 600
           T    DNR  PF T SGTSMSCPH+SG+VGLL+TLHP WSP+AI+SAIMT++R R+N  K
Sbjct: 556 TDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRK 615

Query: 601 PMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQAFS 660
           PM+D        A PF+YGSGH++P  A  PGLVYDL+  DYL+FLCA GY+   +Q F+
Sbjct: 616 PMVDE---SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 675

Query: 661 -DGHFKCPASASILNFNYPSIGVQNLNGSVTVTRKLKNVSTPGVYKARIRHPNGVKILVK 720
            D  + C   A++L+FNYPSI V NL GS+TVTRKLKNV  P  Y AR R P GV++ V+
Sbjct: 676 EDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVE 735

Query: 721 PKVLKFERVGEEKSFELTITG-DVPENQVVDGVLIWTDGKHFVRSPIVVSSS 770
           PK L F + GE K F++T+    V  +  V G L WTD  H+VRSPIVV  S
Sbjct: 736 PKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

BLAST of PI0025840 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 803.1 bits (2073), Expect = 1.9e-232
Identity = 414/778 (53.21%), Postives = 547/778 (70.31%), Query Frame = 0

Query: 1   MKISNSILILFSLLLI--ISPAIAAKK---SYIVLLGSHSHGLEVTDESLERVVDSHKKL 60
           MK++++   L  LLL+   S  I A K   SY+V  G+HSH  E+T+++++RV ++H   
Sbjct: 1   MKLTHNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDF 60

Query: 61  LGSIFRSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHS 120
           LGS   S E+A DAIFYSY K+INGFAA +D + A +++KHPEV +V PN+A +LHTT S
Sbjct: 61  LGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRS 120

Query: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
           W+F+ LE N  +P SS WR+A+ G+D IIANLDTGVWPESKSF + G+ GP+PS+WKG C
Sbjct: 121 WDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGIC 180

Query: 181 TDKTPDGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYV 240
            ++      CNRKLIGA+YFNKG+ A +   N   S  +S RD DGHGSHTLSTA G +V
Sbjct: 181 QNQKDATFHCNRKLIGARYFNKGYAAAVGHLN---SSFDSPRDLDGHGSHTLSTAAGDFV 240

Query: 241 SGANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAEAFDHAIHDRVDVLSLS 300
            G ++FG G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+S
Sbjct: 241 PGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVS 300

Query: 301 LGGEPAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREF 360
           LGGEP  ++ D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGAST+DREF
Sbjct: 301 LGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREF 360

Query: 361 QAPVELQNGHRYMGSSLSK-GLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVK 420
            + + L NG  Y G SLS   L  +K YP++    AKAKNA+A  A LCK  +LD  K K
Sbjct: 361 ASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTK 420

Query: 421 GKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAV 480
           GKILVCLRG   RV+KG   AL G +GM+L N  ++G + +ADPHVLPA+ +   D  AV
Sbjct: 421 GKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAV 480

Query: 481 YSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAF 540
             YI+ TK P+ ++ P    +  KPAP MA+FSS+GP+ ++P+I+KPD+TAPG ++IAA+
Sbjct: 481 SRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAY 540

Query: 541 SEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARI 600
           + AVSPT E FD R + F  +SGTSMSCPH+SG+ GLL+T +P WSP+AI+SAIMT+A I
Sbjct: 541 TGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATI 600

Query: 601 RDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEK 660
            D+   P+ +  +++   ATPF++G+GH++P  A++PGLVYDL   DYL FLC+ GY+  
Sbjct: 601 MDDIPGPIQNATNMK---ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNAS 660

Query: 661 TIQAFSDGHFKCPA-SASILNFNYPSIGVQNLNGS-VTVTRKLKNVSTPGVYKARIRHPN 720
            I  FS  +F C +   S++N NYPSI V NL  S VTV+R +KNV  P +Y  ++ +P 
Sbjct: 661 QISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQ 720

Query: 721 GVKILVKPKVLKFERVGEEKSFELTIT---GDVPENQVVDGVLIWTDGKHFVRSPIVV 767
           GV + VKP  L F +VGE+K+F++ +    G+V +  V  G L+W+D KH VRSPIVV
Sbjct: 721 GVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

BLAST of PI0025840 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 582.8 bits (1501), Expect = 4.1e-166
Identity = 333/727 (45.80%), Postives = 447/727 (61.49%), Query Frame = 0

Query: 50  DSHKKLLGSIFRSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQ 109
           D H     S  RS   + + + Y+Y+  I+GF+  + +EEA  L   P V +VLP    +
Sbjct: 46  DLHSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYE 105

Query: 110 LHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPS 169
           LHTT +  F+ L+++     +  +  A S  DV++  LDTGVWPESKS+ + G  GP+PS
Sbjct: 106 LHTTRTPLFLGLDEH----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPS 165

Query: 170 KWKGGCTDKTP-DGVPCNRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLS 229
            WKGGC   T      CNRKLIGA++F +G+ + +   +  +    S RD DGHG+HT S
Sbjct: 166 SWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPID-ESKESRSPRDDDGHGTHTSS 225

Query: 230 TAGGSYVSGANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRV 289
           TA GS V GA++ G   GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V
Sbjct: 226 TAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNV 285

Query: 290 DVLSLSLGGEPAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGAS 349
           +VLS+SLGG  ++YY DG+AI AF A+++GI V CSAGN+GP + ++SN APWI TVGA 
Sbjct: 286 NVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAG 345

Query: 350 TLDREFQAPVELQNGHRYMGSSLSKG-LKGSKLYPLITGAEAKAKNATAEVAMLCKPKTL 409
           TLDR+F A   L NG  + G SL KG     KL P I      A NAT     LC   TL
Sbjct: 346 TLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTL 405

Query: 410 DHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINY 469
              KVKGKI++C RG  ARV KG+    AG VGMIL N   +G E +AD H+LPA+ +  
Sbjct: 406 IPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGE 465

Query: 470 NDGQAVYSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGA 529
             G  +  Y+ +  NP   +      V  KP+P +AAFSSRGPN I+P I+KPD+ APG 
Sbjct: 466 KAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGV 525

Query: 530 NIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAI 589
           NI+AA++ A  PTG   D+R V F  +SGTSMSCPHVSGL  LL+++HP+WSP+AI+SA+
Sbjct: 526 NILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSAL 585

Query: 590 MTSARIRDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 649
           MT+A       KP+LD  + +  P+TPF +G+GH+ PT A +PGL+YDL+  DYL FLCA
Sbjct: 586 MTTAYKTYKDGKPLLDIATGK--PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCA 645

Query: 650 SGYDEKTIQAFSDGHFKCPASA--SILNFNYPSIGVQNLN--GSVTVTRKLKNVSTPGVY 709
             Y    I++ S  ++ C  S   S+ + NYPS  V N++  G+   TR + +V   G Y
Sbjct: 646 LNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTY 705

Query: 710 KARI-RHPNGVKILVKPKVLKFERVGEEKSFELTITGD--VPENQVVDGVLIWTDGKHFV 768
             ++     GVKI V+P VL F+   E+KS+ +T T D   P      G + W+DGKH V
Sbjct: 706 SVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVV 755

BLAST of PI0025840 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 552.7 bits (1423), Expect = 4.5e-157
Identity = 325/784 (41.45%), Postives = 458/784 (58.42%), Query Frame = 0

Query: 11  FSLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSIFRSDEKARDAI 70
           F LL + SP+ +A  S      S ++ + V  E+   +  +H     S   S   +  +I
Sbjct: 8   FFLLTLSSPSSSASSS-----NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSI 67

Query: 71  FYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFMHL---EKNGVI 130
            ++Y    +GF+A +  ++A+QL  HP V +V+P + + LHTT S EF+ L   +K G++
Sbjct: 68  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 127

Query: 131 PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC--TDKTPDGVPC 190
             S        G D++I  +DTGVWPE  SF + G+ GPVP KWKG C  +   P+   C
Sbjct: 128 EESD------FGSDLVIGVIDTGVWPERPSFDDRGL-GPVPIKWKGQCIASQDFPESA-C 187

Query: 191 NRKLIGAKYFNKGFLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSGANVFGSGV 250
           NRKL+GA++F  G+ A     N T     S RD DGHG+HT S + G YV  A+  G   
Sbjct: 188 NRKLVGARFFCGGYEATNGKMNETTE-FRSPRDSDGHGTHTASISAGRYVFPASTLGYAH 247

Query: 251 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGGEPAEYYED 310
           G A G +PKAR+AAYKVCW   + GC+D+DI  AFD A+ D VDV+SLS+GG    YY D
Sbjct: 248 GVAAGMAPKARLAAYKVCW---NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLD 307

Query: 311 GIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREFQAPVELQNGHR 370
            IAI AF A+ +GI V  SAGN GPGA TV+N APW+ TVGA T+DR+F A V+L NG  
Sbjct: 308 AIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKM 367

Query: 371 YMGSSL--SKGLKGSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGD 430
             G S+    GL   ++YPL+ G      +  +  + LC   +LD + VKGKI++C RG 
Sbjct: 368 ISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS--SSLCLEGSLDPNLVKGKIVLCDRGI 427

Query: 431 TARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVYSYIN----- 490
            +R  KGE     G +GMI+ N    G   +AD HVLPA+ +  + G  +  YI+     
Sbjct: 428 NSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKS 487

Query: 491 -STKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAFSEAV 550
            S+K+P   ++    ++  +PAP +A+FS+RGPNP +PEI+KPDV APG NI+AA+ + +
Sbjct: 488 RSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRI 547

Query: 551 SPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNT 610
            P+G   DNR   F  +SGTSM+CPHVSGL  LL+  HP WSP+AI+SA++T+A   DN+
Sbjct: 548 GPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNS 607

Query: 611 NKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIQA 670
            +PM+D  +     ++   YGSGH+ PT A+DPGLVYD++  DY+ FLC S Y    I  
Sbjct: 608 GEPMMDESTGN--TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVT 667

Query: 671 FSDGHFKCPA---SASILNFNYPSIGV--QNLNGSVTVTRKLKNVSTPG----VYKARIR 730
            +     C     +  + N NYPS  V  Q    S   T  ++ V+  G    VY+ +IR
Sbjct: 668 ITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIR 727

Query: 731 HPNGVKILVKPKVLKFERVGEEKSFELTI-TGDVP----ENQVVDGVLIWTDGKHFVRSP 768
            P G  + V+P+ L F RVG++ SF + + T +V        V  G ++W+DGK  V SP
Sbjct: 728 PPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSP 770

BLAST of PI0025840 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 550.4 bits (1417), Expect = 2.2e-156
Identity = 329/788 (41.75%), Postives = 458/788 (58.12%), Query Frame = 0

Query: 1   MKISNSILILF-SLLLIISPAIAAKKSYIVLLGSHSHGLEVTDESLERVVDSHKKLLGSI 60
           M  S  +L+LF S   I   A  A K++I           +   S+  +  +H     + 
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFI---------FRIDGGSMPSIFPTHYHWYSTE 60

Query: 61  FRSDEKARDAIFYSYKKNINGFAAIMDEEEATQLAKHPEVAAVLPNRAKQLHTTHSWEFM 120
           F  + +    I + Y    +GF+A++  +EA  L  HP V AV  +R ++LHTT S +F+
Sbjct: 61  FAEESR----IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFL 120

Query: 121 HLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT 180
            L+          W  +  G DVII   DTG+WPE +SF +  + GP+P +W+G C    
Sbjct: 121 GLQNQ-----KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGA 180

Query: 181 P-DGVPCNRKLIGAKYFNKG-FLAYLKSENLTASVINSTRDYDGHGSHTLSTAGGSYVSG 240
                 CNRK+IGA++F KG   A +   N T   + S RD DGHG+HT STA G +   
Sbjct: 181 RFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFL-SPRDADGHGTHTSSTAAGRHAFK 240

Query: 241 ANVFGSGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAEAFDHAIHDRVDVLSLSLGG 300
           A++ G   G AKG +PKAR+AAYKVCW  +D GC D+DI  AFD A+ D VDV+S+S+GG
Sbjct: 241 ASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGG 300

Query: 301 E---PAEYYEDGIAIAAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTLDREF 360
                + YY D IAI ++ A  KGI V  SAGN GP   +V+N APW+ TVGAST+DR F
Sbjct: 301 GDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 360

Query: 361 QAPVELQNGHRYMGSSLSKGLK-GSKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVK 420
            A   L +GHR  G SL  G+    +++P++   ++   +A+     LC   TLD  +V+
Sbjct: 361 PADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVR 420

Query: 421 GKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAV 480
           GKI++C RG + RV KG     AG VGMIL N   +G   + D H++PA  +  N+G  +
Sbjct: 421 GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRI 480

Query: 481 YSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNPISPEIIKPDVTAPGANIIAAF 540
            +Y +S  NP+  +      V  KPAP +A+FS RGPN +SPEI+KPD+ APG NI+AA+
Sbjct: 481 KAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAW 540

Query: 541 SEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARI 600
           ++AV PTG P D R   F  +SGTSM+CPHVSG   LL++ HP WSP+ I+SA+MT+  +
Sbjct: 541 TDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNL 600

Query: 601 RDNTNKPMLDGGSLELAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDEK 660
            DN+N+ ++D  + +   ATP+ YGSGH+    A++PGLVYD++ +DY+ FLC+ GY  K
Sbjct: 601 VDNSNRSLIDESTGK--SATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPK 660

Query: 661 TIQAFSDGHFKCPAS--ASILNFNYPSIGV---QNLNG--SVTVTRKLKNV-STPGVYKA 720
           TIQ  +    +CP +   S  N NYPSI      N  G  S TV R   NV     VY+A
Sbjct: 661 TIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRA 720

Query: 721 RIRHPNGVKILVKPKVLKFERVGEEKSFELTITGD-----VPENQVVDGVLIWTD-GKHF 768
           RI  P GV + VKP  L F    + +S+ +T+T +     + E   V G + W D GKH 
Sbjct: 721 RIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHV 759