PI0025765 (gene) Melon (PI 482460) v1

Overview
NamePI0025765
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPeroxyureidoacrylate/ureidoacrylate amidohydrolase RutB
Locationchr01: 27775429 .. 27777340 (-)
RNA-Seq ExpressionPI0025765
SyntenyPI0025765
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAAATCGTACAATTATCTGATTTTCGAATTACTTCATCTGTCGTCTTACGGAACTTTAGTGCCATCTTCATCCCTCCTCACAAAATTTCATCATCTTTTCTCTTTGAGCTTCCTTTTAGCTATGGCGGATCAATGGAAACGCACTGCTCTTCTTGTTATCGACATGCAGGTTTTTTTCCCTTTTATTTTCTTCCTAATTCAATCCACTTCTCTATTCCCATAATCAATCTCTTTCTTCCAATTCTTCGTTACAGAGAGATTTCATTGACGATCACTCCGTCTTCGCTGTACCAGGAGCCTCCACCATAATCCCAAGCGTCTCCAATGCTGTCGAAATCGCAAGGAATCGCGGGTTGTTCATCATTTGGGTCCGTTAATTTTGAAGAGAAAAAAATCCCTAATCCTCTGTTTGGACGGATTTCAAATCGTGAATTGAATTTCTGTTTGTTTTAACCTAGGTTGTTCGAGAACATGATGGTGAAGGACGAGATGTTGAACTATTTCGCCGCCATTACTATGGGAATGGGAAGTCGAATCCTCTCGTGAAAGGAAGCAAAGGGGCAGAGTTAGTACAAGGGCTTGAAATTAAAGAAGGAGAATATAAATTGGTTAAAACTAGGTTCAGTGCCTTCTTTAATACTAATTTACATTCTCTTCTTCAAGGGGCGGGTATTACTGATTTGGTTGTCTCTGGTAACATTCTTTATATATACATATTTCCTGTTAATTCCATTCCAATATGAATTTGTAGATTGTATGGTTAACTGAATTAATATAATGGTAGGTGTTCAAACCCCAAATTGTATCAGACAGACTGTTTTTGATGCTGTAGCTTTGGATTACCATTCTATCACTCTCCTTTATGATGCTACTGCAGCTTCTACTCCCGAAATTCATCATGGTATCTCTAATTTTCTCCTCTATAGCTTTTGAGTTCAGTAATCAGTAGTCATTTGTAATGTTGTAGTTGTTTACTCCCAAATTGATATTGACATTCACTTGTTAGACCTCTAAGACTGATTAAGTATTGGTACGATGTTAGAGCCTGAGCCTTAGTTAGAGATATTAGAGATGCAAATTAGTAAATAGCTAGTAAGTTGGTTAGGGCTTTTAGTTATGAATAAGAGGGAATAAGGTTGGAAGAAGGGCGTGAAGAATTTTGAGGGATCTTTTGTGGAGAATTTGGATTAGCCCTTTCGAAAGGCTAGGTTATCTTGTTACATTTCATATACTCGAGTGTTCTTTGTTTGTTCTTGTCAGAATATCCTAACATATAAAAATTTAACTAACCTGTCGATTTGAGTTTTTGGATTGATTAGTGATTGATATACCTTTTTGTACGTTGATGATTGATATTGAATCTCAAGATAAATGTTCTTGGTATGTTTCAGGTAATATCACTGACATGGAGAACGTGGGAGTTGTGGTGAAGAGAGTTGATGAATGGGGGGAGTCTGATTCTGATGCTTGAACCAACCCAATTCTGCCATGGGAATGAATGAAGTTCCAGTTCTAATACATCTTCGTGTTGCTGGTGATTTTCATCTACTGCAACGAATCCATGGTTTGATCTTCAACCATGCTTCTTCTGTTTCTTTTTTCCACCGTGTAACCACATTCATATAGAAACAATCTTTATCTCTCCCTCTTGCTTTGAGCTTTCAAGAACTTTGATTCTCCTTCTGTATAATTAAAAAAAGAAAAAACAAGCTTACTTTTTCATCGAAGTGAAATAATCTCTCTCTCTCTCTCTCTGCACTTATAACTCTTTTTTATGAGAATTTGATTTTGTGTAAAATTTTATATATGTAATAAACTCCATATAAACTTAGTTTTTTCATCATAAACTTTTAGATGTAATTGATACTATATATCTATTTTGTTTATCTAAAAAATTTATGTCTTATGTC

mRNA sequence

GTCAAATCGTACAATTATCTGATTTTCGAATTACTTCATCTGTCGTCTTACGGAACTTTAGTGCCATCTTCATCCCTCCTCACAAAATTTCATCATCTTTTCTCTTTGAGCTTCCTTTTAGCTATGGCGGATCAATGGAAACGCACTGCTCTTCTTGTTATCGACATGCAGAGAGATTTCATTGACGATCACTCCGTCTTCGCTGTACCAGGAGCCTCCACCATAATCCCAAGCGTCTCCAATGCTGTCGAAATCGCAAGGAATCGCGGGTTGTTCATCATTTGGGTTGTTCGAGAACATGATGGTGAAGGACGAGATGTTGAACTATTTCGCCGCCATTACTATGGGAATGGGAAGTCGAATCCTCTCGTGAAAGGAAGCAAAGGGGCAGAGTTAGTACAAGGGCTTGAAATTAAAGAAGGAGAATATAAATTGGTTAAAACTAGGTTCAGTGCCTTCTTTAATACTAATTTACATTCTCTTCTTCAAGGGGCGGGTATTACTGATTTGGTTGTCTCTGGTGTTCAAACCCCAAATTGTATCAGACAGACTGTTTTTGATGCTGTAGCTTTGGATTACCATTCTATCACTCTCCTTTATGATGCTACTGCAGCTTCTACTCCCGAAATTCATCATGGTAATATCACTGACATGGAGAACGTGGGAGTTGTGGTGAAGAGAGTTGATGAATGGGGGGAGTCTGATTCTGATGCTTGAACCAACCCAATTCTGCCATGGGAATGAATGAAGTTCCAGTTCTAATACATCTTCGTGTTGCTGGTGATTTTCATCTACTGCAACGAATCCATGGTTTGATCTTCAACCATGCTTCTTCTGTTTCTTTTTTCCACCGTGTAACCACATTCATATAGAAACAATCTTTATCTCTCCCTCTTGCTTTGAGCTTTCAAGAACTTTGATTCTCCTTCTGTATAATTAAAAAAAGAAAAAACAAGCTTACTTTTTCATCGAAGTGAAATAATCTCTCTCTCTCTCTCTCTGCACTTATAACTCTTTTTTATGAGAATTTGATTTTGTGTAAAATTTTATATATGTAATAAACTCCATATAAACTTAGTTTTTTCATCATAAACTTTTAGATGTAATTGATACTATATATCTATTTTGTTTATCTAAAAAATTTATGTCTTATGTC

Coding sequence (CDS)

ATGGCGGATCAATGGAAACGCACTGCTCTTCTTGTTATCGACATGCAGAGAGATTTCATTGACGATCACTCCGTCTTCGCTGTACCAGGAGCCTCCACCATAATCCCAAGCGTCTCCAATGCTGTCGAAATCGCAAGGAATCGCGGGTTGTTCATCATTTGGGTTGTTCGAGAACATGATGGTGAAGGACGAGATGTTGAACTATTTCGCCGCCATTACTATGGGAATGGGAAGTCGAATCCTCTCGTGAAAGGAAGCAAAGGGGCAGAGTTAGTACAAGGGCTTGAAATTAAAGAAGGAGAATATAAATTGGTTAAAACTAGGTTCAGTGCCTTCTTTAATACTAATTTACATTCTCTTCTTCAAGGGGCGGGTATTACTGATTTGGTTGTCTCTGGTGTTCAAACCCCAAATTGTATCAGACAGACTGTTTTTGATGCTGTAGCTTTGGATTACCATTCTATCACTCTCCTTTATGATGCTACTGCAGCTTCTACTCCCGAAATTCATCATGGTAATATCACTGACATGGAGAACGTGGGAGTTGTGGTGAAGAGAGTTGATGAATGGGGGGAGTCTGATTCTGATGCTTGA

Protein sequence

MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHDGEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSLLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENVGVVVKRVDEWGESDSDA
Homology
BLAST of PI0025765 vs. ExPASy Swiss-Prot
Match: Q93Z51 (Probable inactive nicotinamidase At3g16190 OS=Arabidopsis thaliana OX=3702 GN=At3g16190 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 2.4e-67
Identity = 122/193 (63.21%), Postives = 155/193 (80.31%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MA++W+ TALLVIDMQ DFI++ +V  V G  +I+P+V   VE+AR RG+ +IWVVREHD
Sbjct: 1   MAERWRNTALLVIDMQNDFIEEGAVTQVKGGKSIVPNVIRVVELARQRGILVIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEI-KEGEYKLVKTRFSAFFNTNLHS 120
            +GRDVELFRRH Y + K  P++KG+ GAELV GL I +E +YK+VKTRFSAFF+TNLHS
Sbjct: 61  RQGRDVELFRRHNYSSEKVGPVIKGTVGAELVDGLMINEEDDYKIVKTRFSAFFSTNLHS 120

Query: 121 LLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMEN 180
            LQ +G+T LV++GVQTPNCIRQTVFDAVALDY ++T++ DATAA+TPEIH  NI DM+N
Sbjct: 121 FLQTSGVTKLVIAGVQTPNCIRQTVFDAVALDYPNVTVITDATAAATPEIHTANILDMKN 180

Query: 181 VGVVVKRVDEWGE 193
           +GV    + EW E
Sbjct: 181 IGVKTPTLHEWSE 193

BLAST of PI0025765 vs. ExPASy Swiss-Prot
Match: D5VGV1 (Ureidoacrylate amidohydrolase RutB OS=Caulobacter segnis (strain ATCC 21756 / DSM 7131 / JCM 7823 / NBRC 15250 / LMG 17158 / TK0059) OX=509190 GN=rutB PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.8e-15
Identity = 57/180 (31.67%), Postives = 91/180 (50.56%), Query Frame = 0

Query: 6   KRTALLVIDMQRDFID-----DHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 65
           K TA++VIDMQ  +       D + F + GA+ +I  +   +E+AR+ G+ +I+     D
Sbjct: 27  KTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVIHEIKGVLEVARSAGMQVIYFQNGWD 86

Query: 66  ------GEGRDVELFRRHYYGNGKSNP------LVKGSKGAELVQGLEIKEGEYKLVKTR 125
                 G       ++ +     ++ P      L +G    ELV  L+ + G+ +L KTR
Sbjct: 87  DQYVEAGGPGSPNWWKSNALKTMRAKPELQGKLLARGQWDYELVDELKPQPGDIQLHKTR 146

Query: 126 FSAFFNTNLHSLLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPE 169
           +S FFN+ L S+L+  GI  LV  G+ T  C+  T+ D   L+Y   T+L DAT  + PE
Sbjct: 147 YSGFFNSQLDSVLRARGIRHLVFVGIATNVCVESTLRDGFFLEYFG-TVLEDATHQAGPE 205

BLAST of PI0025765 vs. ExPASy Swiss-Prot
Match: Q9A4N5 (Ureidoacrylate amidohydrolase RutB OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=rutB PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 55/180 (30.56%), Postives = 90/180 (50.00%), Query Frame = 0

Query: 6   KRTALLVIDMQRDFID-----DHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 65
           K TA++VIDMQ  +       D + F + GA+ +   +   +E+AR+ G+ +I+     D
Sbjct: 14  KTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVTHEIKGVLEVARSAGMTVIYFQNGWD 73

Query: 66  ------GEGRDVELFRRHYYGNGKSNP------LVKGSKGAELVQGLEIKEGEYKLVKTR 125
                 G       ++ +     ++ P      L +G    ELV  L  + G+ +L KTR
Sbjct: 74  DGYVEAGGPGSPNWWKSNALKTMRARPELQGKLLARGQWDYELVDDLTPQPGDIRLHKTR 133

Query: 126 FSAFFNTNLHSLLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPE 169
           +S FFN+ L S+L+  GI  LV +G+ T  C+  T+ D   L+Y   T+L DAT  + P+
Sbjct: 134 YSGFFNSQLDSVLRARGIRHLVFTGIATNVCVESTLRDGFMLEYFG-TVLEDATHQAGPD 192

BLAST of PI0025765 vs. ExPASy Swiss-Prot
Match: B8H1Q1 (Ureidoacrylate amidohydrolase RutB OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565050 GN=rutB PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 55/180 (30.56%), Postives = 90/180 (50.00%), Query Frame = 0

Query: 6   KRTALLVIDMQRDFID-----DHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 65
           K TA++VIDMQ  +       D + F + GA+ +   +   +E+AR+ G+ +I+     D
Sbjct: 27  KTTAVIVIDMQNAYASPGGYLDLAGFDISGAAKVTHEIKGVLEVARSAGMTVIYFQNGWD 86

Query: 66  ------GEGRDVELFRRHYYGNGKSNP------LVKGSKGAELVQGLEIKEGEYKLVKTR 125
                 G       ++ +     ++ P      L +G    ELV  L  + G+ +L KTR
Sbjct: 87  DGYVEAGGPGSPNWWKSNALKTMRARPELQGKLLARGQWDYELVDDLTPQPGDIRLHKTR 146

Query: 126 FSAFFNTNLHSLLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPE 169
           +S FFN+ L S+L+  GI  LV +G+ T  C+  T+ D   L+Y   T+L DAT  + P+
Sbjct: 147 YSGFFNSQLDSVLRARGIRHLVFTGIATNVCVESTLRDGFMLEYFG-TVLEDATHQAGPD 205

BLAST of PI0025765 vs. ExPASy Swiss-Prot
Match: B7NLB5 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) OX=585057 GN=rutB PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 59/182 (32.42%), Postives = 88/182 (48.35%), Query Frame = 0

Query: 6   KRTALLVIDMQRDFID-----DHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 65
           ++TAL+V+DMQ  +       D + F V     +I ++  AV  AR  G+ IIW     D
Sbjct: 16  QQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWD 75

Query: 66  GE--------------GRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVK 125
            +                 ++  R+     GK   L KGS   +LV  L  + G+  L K
Sbjct: 76  AQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKL--LAKGSWDYQLVDELVPQPGDIVLPK 135

Query: 126 TRFSAFFNTNLHSLLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAAST 169
            R+S+FFNT L S+L+  GI  LV +G+ T  C+  T+ D   L+Y  + +L DAT  + 
Sbjct: 136 PRYSSFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLEDATHQAG 194

BLAST of PI0025765 vs. ExPASy TrEMBL
Match: A0A1S3CP26 (probable inactive nicotinamidase At3g16190 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503139 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 8.5e-100
Identity = 184/197 (93.40%), Postives = 189/197 (95.94%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MADQWKRTALLVIDMQRDFID+HSVFAVPGASTIIPSVS AVEIARNRGLFIIWVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           GEGRDVE FRRHYYGNGK NPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENV 180
           LQGAGITDLVV GVQTPNCIRQTVFDAVALDYHSITLLYDATAA+TP++HH NITDM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGESDSDA 198
           GV VKRVDEWGESDS+A
Sbjct: 181 GVEVKRVDEWGESDSEA 197

BLAST of PI0025765 vs. ExPASy TrEMBL
Match: A0A5A7UTR3 (Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G00370 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.9e-99
Identity = 183/197 (92.89%), Postives = 189/197 (95.94%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MADQWKRTALLVIDMQRDFID+HSVFAVPGASTIIPSVS AVEIARNRGLFIIWVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           GEGRDVE FRRHYYGNGK NPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENV 180
           LQGAGITDLVV GVQTPNCIRQTV+DAVALDYHSITLLYDATAA+TP++HH NITDM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVYDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGESDSDA 198
           GV VKRVDEWGESDS+A
Sbjct: 181 GVEVKRVDEWGESDSEA 197

BLAST of PI0025765 vs. ExPASy TrEMBL
Match: A0A0A0K7E5 (Isochorismatase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447030 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.1e-96
Identity = 176/192 (91.67%), Postives = 182/192 (94.79%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           M DQWKRTALLVIDMQ DF D+HS FAVPGASTIIPSVSNA+EIARNRGLFIIWVVREHD
Sbjct: 1   MTDQWKRTALLVIDMQTDFFDEHSAFAVPGASTIIPSVSNAIEIARNRGLFIIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           GEGRDVE FRRHYYGNGK NP VKGSKGAELV+GLEIKEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPSVKGSKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENV 180
           LQGAGITDLVV GVQTPNCIRQTVFDAVALDYHSITLLYDATAA+TP+IHH N TDMENV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKIHHDNTTDMENV 180

Query: 181 GVVVKRVDEWGE 193
           GVVVKRVD+WGE
Sbjct: 181 GVVVKRVDQWGE 192

BLAST of PI0025765 vs. ExPASy TrEMBL
Match: A0A5D3BXX3 (Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002130 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.8e-95
Identity = 183/224 (81.70%), Postives = 189/224 (84.38%), Query Frame = 0

Query: 1   MADQWKRTALLVIDM---------------------------QRDFIDDHSVFAVPGAST 60
           MADQWKRTALLVIDM                           QRDFID+HSVFAVPGAST
Sbjct: 1   MADQWKRTALLVIDMQVXPFYFLPNSIPFSTPTIILFNFFQLQRDFIDEHSVFAVPGAST 60

Query: 61  IIPSVSNAVEIARNRGLFIIWVVREHDGEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQ 120
           IIPSVS AVEIARNRGLFIIWVVREHDGEGRDVE FRRHYYGNGK NPLVKGSKGAELVQ
Sbjct: 61  IIPSVSTAVEIARNRGLFIIWVVREHDGEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQ 120

Query: 121 GLEIKEGEYKLVKTRFSAFFNTNLHSLLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYH 180
           GLEIKEGEYKLVKTRFSAFFNTNL SLLQGAGITDLVV GVQTPNCIRQTV+DAVALDYH
Sbjct: 121 GLEIKEGEYKLVKTRFSAFFNTNLRSLLQGAGITDLVVCGVQTPNCIRQTVYDAVALDYH 180

Query: 181 SITLLYDATAASTPEIHHGNITDMENVGVVVKRVDEWGESDSDA 198
           SITLLYDATAA+TP++HH NITDM NVGV VKRVDEWGESDS+A
Sbjct: 181 SITLLYDATAAATPKVHHDNITDMVNVGVEVKRVDEWGESDSEA 224

BLAST of PI0025765 vs. ExPASy TrEMBL
Match: A0A1S4E593 (probable inactive nicotinamidase At3g16190 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503139 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.8e-87
Identity = 162/171 (94.74%), Postives = 166/171 (97.08%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MADQWKRTALLVIDMQRDFID+HSVFAVPGASTIIPSVS AVEIARNRGLFIIWVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           GEGRDVE FRRHYYGNGK NPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHH 172
           LQGAGITDLVV GVQTPNCIRQTVFDAVALDYHSITLLYDATAA+TP++HH
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKVHH 171

BLAST of PI0025765 vs. NCBI nr
Match: XP_008465520.1 (PREDICTED: probable inactive nicotinamidase At3g16190 isoform X1 [Cucumis melo])

HSP 1 Score: 372.9 bits (956), Expect = 1.8e-99
Identity = 184/197 (93.40%), Postives = 189/197 (95.94%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MADQWKRTALLVIDMQRDFID+HSVFAVPGASTIIPSVS AVEIARNRGLFIIWVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           GEGRDVE FRRHYYGNGK NPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENV 180
           LQGAGITDLVV GVQTPNCIRQTVFDAVALDYHSITLLYDATAA+TP++HH NITDM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGESDSDA 198
           GV VKRVDEWGESDS+A
Sbjct: 181 GVEVKRVDEWGESDSEA 197

BLAST of PI0025765 vs. NCBI nr
Match: KAA0059313.1 (putative inactive nicotinamidase [Cucumis melo var. makuwa])

HSP 1 Score: 371.7 bits (953), Expect = 3.9e-99
Identity = 183/197 (92.89%), Postives = 189/197 (95.94%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MADQWKRTALLVIDMQRDFID+HSVFAVPGASTIIPSVS AVEIARNRGLFIIWVVREHD
Sbjct: 1   MADQWKRTALLVIDMQRDFIDEHSVFAVPGASTIIPSVSTAVEIARNRGLFIIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           GEGRDVE FRRHYYGNGK NPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNL SL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLRSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENV 180
           LQGAGITDLVV GVQTPNCIRQTV+DAVALDYHSITLLYDATAA+TP++HH NITDM NV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVYDAVALDYHSITLLYDATAAATPKVHHDNITDMVNV 180

Query: 181 GVVVKRVDEWGESDSDA 198
           GV VKRVDEWGESDS+A
Sbjct: 181 GVEVKRVDEWGESDSEA 197

BLAST of PI0025765 vs. NCBI nr
Match: XP_004141764.1 (probable inactive nicotinamidase At3g16190 [Cucumis sativus] >KGN45388.1 hypothetical protein Csa_015610 [Cucumis sativus])

HSP 1 Score: 362.5 bits (929), Expect = 2.4e-96
Identity = 176/192 (91.67%), Postives = 182/192 (94.79%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           M DQWKRTALLVIDMQ DF D+HS FAVPGASTIIPSVSNA+EIARNRGLFIIWVVREHD
Sbjct: 1   MTDQWKRTALLVIDMQTDFFDEHSAFAVPGASTIIPSVSNAIEIARNRGLFIIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
           GEGRDVE FRRHYYGNGK NP VKGSKGAELV+GLEIKEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  GEGRDVERFRRHYYGNGKPNPSVKGSKGAELVEGLEIKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENV 180
           LQGAGITDLVV GVQTPNCIRQTVFDAVALDYHSITLLYDATAA+TP+IHH N TDMENV
Sbjct: 121 LQGAGITDLVVCGVQTPNCIRQTVFDAVALDYHSITLLYDATAAATPKIHHDNTTDMENV 180

Query: 181 GVVVKRVDEWGE 193
           GVVVKRVD+WGE
Sbjct: 181 GVVVKRVDQWGE 192

BLAST of PI0025765 vs. NCBI nr
Match: TYK04015.1 (putative inactive nicotinamidase [Cucumis melo var. makuwa])

HSP 1 Score: 357.1 bits (915), Expect = 1.0e-94
Identity = 183/224 (81.70%), Postives = 189/224 (84.38%), Query Frame = 0

Query: 1   MADQWKRTALLVIDM---------------------------QRDFIDDHSVFAVPGAST 60
           MADQWKRTALLVIDM                           QRDFID+HSVFAVPGAST
Sbjct: 1   MADQWKRTALLVIDMQVXPFYFLPNSIPFSTPTIILFNFFQLQRDFIDEHSVFAVPGAST 60

Query: 61  IIPSVSNAVEIARNRGLFIIWVVREHDGEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQ 120
           IIPSVS AVEIARNRGLFIIWVVREHDGEGRDVE FRRHYYGNGK NPLVKGSKGAELVQ
Sbjct: 61  IIPSVSTAVEIARNRGLFIIWVVREHDGEGRDVERFRRHYYGNGKPNPLVKGSKGAELVQ 120

Query: 121 GLEIKEGEYKLVKTRFSAFFNTNLHSLLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYH 180
           GLEIKEGEYKLVKTRFSAFFNTNL SLLQGAGITDLVV GVQTPNCIRQTV+DAVALDYH
Sbjct: 121 GLEIKEGEYKLVKTRFSAFFNTNLRSLLQGAGITDLVVCGVQTPNCIRQTVYDAVALDYH 180

Query: 181 SITLLYDATAASTPEIHHGNITDMENVGVVVKRVDEWGESDSDA 198
           SITLLYDATAA+TP++HH NITDM NVGV VKRVDEWGESDS+A
Sbjct: 181 SITLLYDATAAATPKVHHDNITDMVNVGVEVKRVDEWGESDSEA 224

BLAST of PI0025765 vs. NCBI nr
Match: XP_038898995.1 (probable inactive nicotinamidase At3g16190 [Benincasa hispida])

HSP 1 Score: 341.3 bits (874), Expect = 5.7e-90
Identity = 166/191 (86.91%), Postives = 179/191 (93.72%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MADQ K TALLVIDMQ+DF+D+ SVFAVPGA+TI+PSV N VEIARNRGLFI+WVVREHD
Sbjct: 1   MADQLKHTALLVIDMQKDFLDEQSVFAVPGATTILPSVINTVEIARNRGLFIVWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEIKEGEYKLVKTRFSAFFNTNLHSL 120
            +GRDVE FRRHYYG+GK NP+ KGSKGAELV+GLE+KEGEYKLVKTRFSAFFNTNLHSL
Sbjct: 61  PQGRDVERFRRHYYGSGKPNPVSKGSKGAELVEGLEMKEGEYKLVKTRFSAFFNTNLHSL 120

Query: 121 LQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMENV 180
           LQG GITDLVVSGVQTPNCIRQTVFDAVALDYHSITLL DATAA+TP+IHHGNITDMENV
Sbjct: 121 LQGMGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLSDATAAATPKIHHGNITDMENV 180

Query: 181 GVVVKRVDEWG 192
           GVVV RVDEWG
Sbjct: 181 GVVVMRVDEWG 191

BLAST of PI0025765 vs. TAIR 10
Match: AT3G16190.1 (Isochorismatase family protein )

HSP 1 Score: 256.5 bits (654), Expect = 1.7e-68
Identity = 122/193 (63.21%), Postives = 155/193 (80.31%), Query Frame = 0

Query: 1   MADQWKRTALLVIDMQRDFIDDHSVFAVPGASTIIPSVSNAVEIARNRGLFIIWVVREHD 60
           MA++W+ TALLVIDMQ DFI++ +V  V G  +I+P+V   VE+AR RG+ +IWVVREHD
Sbjct: 1   MAERWRNTALLVIDMQNDFIEEGAVTQVKGGKSIVPNVIRVVELARQRGILVIWVVREHD 60

Query: 61  GEGRDVELFRRHYYGNGKSNPLVKGSKGAELVQGLEI-KEGEYKLVKTRFSAFFNTNLHS 120
            +GRDVELFRRH Y + K  P++KG+ GAELV GL I +E +YK+VKTRFSAFF+TNLHS
Sbjct: 61  RQGRDVELFRRHNYSSEKVGPVIKGTVGAELVDGLMINEEDDYKIVKTRFSAFFSTNLHS 120

Query: 121 LLQGAGITDLVVSGVQTPNCIRQTVFDAVALDYHSITLLYDATAASTPEIHHGNITDMEN 180
            LQ +G+T LV++GVQTPNCIRQTVFDAVALDY ++T++ DATAA+TPEIH  NI DM+N
Sbjct: 121 FLQTSGVTKLVIAGVQTPNCIRQTVFDAVALDYPNVTVITDATAAATPEIHTANILDMKN 180

Query: 181 VGVVVKRVDEWGE 193
           +GV    + EW E
Sbjct: 181 IGVKTPTLHEWSE 193

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z512.4e-6763.21Probable inactive nicotinamidase At3g16190 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
D5VGV14.8e-1531.67Ureidoacrylate amidohydrolase RutB OS=Caulobacter segnis (strain ATCC 21756 / DS... [more]
Q9A4N51.1e-1430.56Ureidoacrylate amidohydrolase RutB OS=Caulobacter vibrioides (strain ATCC 19089 ... [more]
B8H1Q11.1e-1430.56Ureidoacrylate amidohydrolase RutB OS=Caulobacter vibrioides (strain NA1000 / CB... [more]
B7NLB51.1e-1432.42Ureidoacrylate amidohydrolase RutB OS=Escherichia coli O7:K1 (strain IAI39 / ExP... [more]
Match NameE-valueIdentityDescription
A0A1S3CP268.5e-10093.40probable inactive nicotinamidase At3g16190 isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A5A7UTR31.9e-9992.89Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A0A0K7E51.1e-9691.67Isochorismatase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G44... [more]
A0A5D3BXX34.8e-9581.70Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S4E5934.8e-8794.74probable inactive nicotinamidase At3g16190 isoform X2 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
XP_008465520.11.8e-9993.40PREDICTED: probable inactive nicotinamidase At3g16190 isoform X1 [Cucumis melo][more]
KAA0059313.13.9e-9992.89putative inactive nicotinamidase [Cucumis melo var. makuwa][more]
XP_004141764.12.4e-9691.67probable inactive nicotinamidase At3g16190 [Cucumis sativus] >KGN45388.1 hypothe... [more]
TYK04015.11.0e-9481.70putative inactive nicotinamidase [Cucumis melo var. makuwa][more]
XP_038898995.15.7e-9086.91probable inactive nicotinamidase At3g16190 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G16190.11.7e-6863.21Isochorismatase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036380Isochorismatase-like superfamilyGENE3D3.40.50.850coord: 2..195
e-value: 4.3E-52
score: 178.9
IPR036380Isochorismatase-like superfamilySUPERFAMILY52499Isochorismatase-like hydrolasescoord: 5..190
IPR000868Isochorismatase-likePFAMPF00857Isochorismatasecoord: 8..184
e-value: 3.8E-37
score: 128.1
NoneNo IPR availablePANTHERPTHR47044OS02G0276400 PROTEINcoord: 1..192
NoneNo IPR availableCDDcd00431cysteine_hydrolasescoord: 9..178
e-value: 8.56102E-43
score: 139.326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025765.1PI0025765.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane